Holger Erfle

Summary

Affiliation: University of Heidelberg
Country: Germany

Publications

  1. doi request reprint Targeting mitosis-regulating genes in cisplatin-sensitive and -resistant melanoma cells: A live-cell RNAi screen displays differential nucleus-derived phenotypes
    Holger Erfle
    BIOQUANT, Heidelberg University, Heidelberg, Germany
    Biotechnol J 10:1467-77. 2015
  2. doi request reprint Cell arrays and high-content screening
    Holger Erfle
    BIOQUANT, University of Heidelberg, Heidelberg, Germany
    Methods Mol Biol 785:277-87. 2011
  3. doi request reprint Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates
    Holger Erfle
    Cell Biology Biophysics Unit, EMBL, Heidelberg, Germany
    J Biomol Screen 13:575-80. 2008
  4. doi request reprint Cell arrays for the measurement of organelle dynamics in living cells
    Holger Erfle
    BIOQUANT, University of Heidelberg, Heidelberg, Germany
    Methods Mol Biol 706:73-81. 2011
  5. pmc Normalizing for individual cell population context in the analysis of high-content cellular screens
    Bettina Knapp
    Heidelberg University, Viroquant Research Group Modeling, Bioquant BQ26, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany
    BMC Bioinformatics 12:485. 2011
  6. pmc miR-17-5p regulates endocytic trafficking through targeting TBC1D2/Armus
    Andrius Serva
    BIOQUANT, University of Heidelberg, Heidelberg, Germany
    PLoS ONE 7:e52555. 2012
  7. pmc Live-cell assays to identify regulators of ER-to-Golgi trafficking
    Tautvydas Lisauskas
    BIOQUANT, University of Heidelberg, 69120, Heidelberg, Germany
    Traffic 13:416-32. 2012
  8. ncbi request reprint Reverse transfection on cell arrays for high content screening microscopy
    Holger Erfle
    MitoCheck Project Group, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Protoc 2:392-9. 2007
  9. ncbi request reprint Measuring secretory membrane traffic: a quantitative fluorescence microscopy approach
    Vytaute Starkuviene
    University of Heidelberg, Heidelberg, Germany
    Methods Mol Biol 457:193-201. 2008
  10. ncbi request reprint Arrays of transfected mammalian cells for high content screening microscopy
    Holger Erfle
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:1-8. 2005

Collaborators

Detail Information

Publications33

  1. doi request reprint Targeting mitosis-regulating genes in cisplatin-sensitive and -resistant melanoma cells: A live-cell RNAi screen displays differential nucleus-derived phenotypes
    Holger Erfle
    BIOQUANT, Heidelberg University, Heidelberg, Germany
    Biotechnol J 10:1467-77. 2015
    ..We hereby show that siRNA screening may help to identify tumor-specific genes leading to phenotype abnormalities. These genes may serve as potential therapeutic targets in the treatment of melanoma. ..
  2. doi request reprint Cell arrays and high-content screening
    Holger Erfle
    BIOQUANT, University of Heidelberg, Heidelberg, Germany
    Methods Mol Biol 785:277-87. 2011
    ..The approach can be modified to quantify endocytosis of other cargo, and can be used under the conditions of knock-down and knock-in as well as for chemical screening...
  3. doi request reprint Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates
    Holger Erfle
    Cell Biology Biophysics Unit, EMBL, Heidelberg, Germany
    J Biomol Screen 13:575-80. 2008
    ..We conclude that solid-phase reverse transfection in multiwell plates is a cost-efficient and flexible tool for multiplexing cellular assays...
  4. doi request reprint Cell arrays for the measurement of organelle dynamics in living cells
    Holger Erfle
    BIOQUANT, University of Heidelberg, Heidelberg, Germany
    Methods Mol Biol 706:73-81. 2011
    ..The approach can be applied for large-scale RNA interference studies as well as for chemical screening...
  5. pmc Normalizing for individual cell population context in the analysis of high-content cellular screens
    Bettina Knapp
    Heidelberg University, Viroquant Research Group Modeling, Bioquant BQ26, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany
    BMC Bioinformatics 12:485. 2011
    ..However, standard analysis methods for cellular screens do not currently take individual cell data into account unless this is important for the phenotype of interest, i.e. when studying cell morphology...
  6. pmc miR-17-5p regulates endocytic trafficking through targeting TBC1D2/Armus
    Andrius Serva
    BIOQUANT, University of Heidelberg, Heidelberg, Germany
    PLoS ONE 7:e52555. 2012
    ..Our study reveals regulation of endocytic trafficking as a novel function of miR-17, which might act cooperatively with other functions of miR-17 and related miRNAs in health and disease...
  7. pmc Live-cell assays to identify regulators of ER-to-Golgi trafficking
    Tautvydas Lisauskas
    BIOQUANT, University of Heidelberg, 69120, Heidelberg, Germany
    Traffic 13:416-32. 2012
    ..Moreover, we show that our assays can be extended to RNAi and chemical screens...
  8. ncbi request reprint Reverse transfection on cell arrays for high content screening microscopy
    Holger Erfle
    MitoCheck Project Group, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Protoc 2:392-9. 2007
    ..Different human cell lines that have been successfully transfected using the protocol are presented here. The present protocol has been applied to two genome-wide siRNA screens addressing mitosis and constitutive protein secretion...
  9. ncbi request reprint Measuring secretory membrane traffic: a quantitative fluorescence microscopy approach
    Vytaute Starkuviene
    University of Heidelberg, Heidelberg, Germany
    Methods Mol Biol 457:193-201. 2008
    ..For each assay the authors discuss protocols for sample preparation, parameters for automated image acquisition, strategies of image analysis, and data quantification...
  10. ncbi request reprint Arrays of transfected mammalian cells for high content screening microscopy
    Holger Erfle
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:1-8. 2005
    ..The method is suitable for high content screening microscopy at a high spatial and temporal resolution allowing even time-lapse analysis of hundreds of samples in parallel...
  11. doi request reprint Next-generation 9216-microwell cell arrays for high-content screening microscopy
    Jürgen Reymann
    ViroQuant CellNetworks RNAi Screening Facility, BIOQUANT, University of Heidelberg, Germany
    Biotechniques 47:877-8. 2009
    ..Here, we present novel 9216-microwell cell arrays which combine the advantages of multiwell plates (physically separated samples) and cell microarrays (high sample density and long-term storage)...
  12. ncbi request reprint Transfected cell microarrays: an efficient tool for high-throughput functional analysis
    Vytaute Starkuviene
    European Molecular Biology Laboratory, Cell Biology and Cell Biophysics Unit, Heidelberg, Germany
    Expert Rev Proteomics 4:479-89. 2007
    ..These advances, combined with an increasing number and diversity of gene perturbing systems, such as RNAi and ectopic gene expression, provide tools for expanding our understanding of biology at the systems level...
  13. ncbi request reprint siRNA cell arrays for high-content screening microscopy
    Holger Erfle
    European Molecular Biology Laboratory, Heidelberg, Germany
    Biotechniques 37:454-8, 460, 462. 2004
    ..We demonstrate such an siRNA cell array in a microscope-based functional assay in living cells to determine the effect of various siRNA oligonucleotides against endogenous targets on cellular secretion...
  14. doi request reprint Identification of cholesterol-regulating genes by targeted RNAi screening
    Fabian Bartz
    Institute of Human Genetics, University of Heidelberg, Seminarstrasse 2, Heidelberg, Germany
    Cell Metab 10:63-75. 2009
    ..Taken together, TMEM97 and other factors described here are promising to yield further insights into how cells control cholesterol levels...
  15. doi request reprint The SUMOylation Pathway Restricts Gene Transduction by Adeno-Associated Viruses
    Christina Hölscher
    German Cancer Research Center, Heidelberg, Germany
    PLoS Pathog 11:e1005281. 2015
    ..We suggest that transiently targeting SUMOylation will enhance application of AAV in vitro and in vivo. ..
  16. pmc Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines
    Kathleen Börner
    Department of Infectious Diseases, Virology, Heidelberg University Hospital, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany, Cluster of Excellence CellNetworks, Heidelberg University, Im Neuenheimer Feld 267, D 69120 Heidelberg, Germany, Department of Medicine III, University Hospital Aachen, Pauwelstrasse 30, D 52074 Aachen, Germany, Department of Infectious Diseases, Molecular Virology, Heidelberg University, Im Neuenheimer Feld 345, D 69120 Heidelberg, Germany, Division Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany, BIOQUANT, Heidelberg University, Im Neuenheimer Feld 267, D 69120 Heidelberg, Germany and Department of Pediatric Oncology, Hematology, Immunology and Pulmonology, Heidelberg University Hospital, Im Neuenheimer Feld 350, D 69120 Heidelberg, Germany
    Nucleic Acids Res 41:e199. 2013
    ..Our customizable reagents and avenues should broadly improve future in vitro and in vivo RNAi experiments in mammalian systems. ..
  17. doi request reprint Correlative light microscopy for high-content screening
    Benjamin Flottmann
    BioQuant Centre, Heidelberg University, Heidelberg, Germany
    Biotechniques 55:243-52. 2013
    ..As a case study, we visualized and quantified cis- and trans-Golgi markers at increasing resolution...
  18. doi request reprint An RNAi screen identifies KIF15 as a novel regulator of the endocytic trafficking of integrin
    Anastasia Eskova
    BIOQUANT, University of Heidelberg, 69120 Heidelberg, Germany
    J Cell Sci 127:2433-47. 2014
    ..Our data suggest a novel role for KIF15 in mediating plasma membrane localization of the alternative clathrin adaptor Dab2, thus impinging on pathways that regulate α2 integrin internalization. ..
  19. ncbi request reprint Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells
    Holger Erfle
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Mol Biol 360:155-61. 2007
    ..This method should facilitate drug target validation by small-interfering RNAs...
  20. doi request reprint Fluorescence microscopy-based RNA interference screening
    Manuel Gunkel
    BIOQUANT, ViroQuant CellNetworks RNAi Screening Facility, Ruprecht Karls Universitat Heidelberg, Im Neuenheimer Feld 267, Heidelberg, 69120, Germany
    Methods Mol Biol 1251:59-66. 2015
    ..Additionally, different microscope types for screening approaches are compared and considerations about the information workflow are made...
  21. doi request reprint Integrated and correlative high-throughput and super-resolution microscopy
    Manuel Gunkel
    BioQuant Centre, Heidelberg University, INF 267, 69120, Heidelberg, Germany
    Histochem Cell Biol 141:597-603. 2014
    ..This procedure is suitable to combine any kind of fluorescence microscopy technique, in order to gain further insights on the observed specimen at multiple temporal or special scales. ..
  22. doi request reprint A three-dimensional colocalization RNA interference screening platform to elucidate the alternative lengthening of telomeres pathway
    Sarah Osterwald
    Research Group Genome Organization and Function, DKFZ and BioQuant, Heidelberg, Germany
    Biotechnol J 7:103-16. 2012
    ..It extends the available repertoire of high-content screening to studies of cellular colocalizations and allows the identification of candidate genes for the ALT mechanism that represent possible targets for cancer therapy...
  23. doi request reprint Single-cell-based image analysis of high-throughput cell array screens for quantification of viral infection
    Petr Matula
    University of Heidelberg, Department of Bioinformatics and Functional Genomics, BIOQUANT, IPMB, Heidelberg, Germany
    Cytometry A 75:309-18. 2009
    ..Our image analysis approach allows for the automatic and accurate determination of changes in viral infection based on high-throughput single-cell-based siRNA cell array imaging experiments...
  24. pmc Identification of HNRNPK as regulator of hepatitis C virus particle production
    Marion Poenisch
    Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany
    PLoS Pathog 11:e1004573. 2015
    ..This study adds a new function to HNRNPK that acts as central hub in the replication cycle of multiple other viruses. ..
  25. pmc The microtubule affinity regulating kinase MARK4 promotes axoneme extension during early ciliogenesis
    Stefanie Kuhns
    Molecular Biology of Centrosomes and Cilia group, Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    J Cell Biol 200:505-22. 2013
    ..We propose that MARK4 is a critical positive regulator of early steps in ciliogenesis...
  26. pmc Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment
    Simon Reiss
    The Department of Infectious Diseases, Molecular Virology, Heidelberg University, 69120 Heidelberg, Germany
    Cell Host Microbe 9:32-45. 2011
    ..Our analysis suggests that the direct activation of a lipid kinase by HCV NS5A contributes critically to the integrity of the membranous viral replication complex...
  27. doi request reprint From experimental setup to bioinformatics: an RNAi screening platform to identify host factors involved in HIV-1 replication
    Kathleen Börner
    Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany
    Biotechnol J 5:39-49. 2010
    ..The ViroQuant-CellNetworks RNAi Screening core facility is an integral part of the recently founded BioQuant centre for systems biology at the University of Heidelberg and will provide service to external users in the near future...
  28. ncbi request reprint An RNAi screening platform to identify secretion machinery in mammalian cells
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biotechnol 129:352-65. 2007
    ..Correlating these findings with other screens and bioinformatic information makes these candidates highly likely to be novel membrane traffic machinery components...
  29. ncbi request reprint High-throughput RNAi screening by time-lapse imaging of live human cells
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Methods 3:385-90. 2006
    ..This modular platform is scalable and makes the power of time-lapse microscopy available for genome-wide RNAi screens...
  30. pmc Automatic identification of subcellular phenotypes on human cell arrays
    Christian Conrad
    Intelligent Bioinformatics Systems, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    Genome Res 14:1130-6. 2004
    ..Our classification method can be adapted to virtually any microscopic assay based on cell morphology, opening a wide range of applications including large-scale RNAi screening in human cells...
  31. ncbi request reprint A microscope-based screening platform for large-scale functional protein analysis in intact cells
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 554:394-8. 2003
    ..Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects...
  32. ncbi request reprint High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol
    Michael Sturzl
    Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen Nuremberg, Schwabachanlage 10, D 91054 Erlangen, Germany
    Comb Chem High Throughput Screen 11:159-72. 2008
    ..Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol...
  33. pmc High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic
    Vytaute Starkuviene
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Genome Res 14:1948-56. 2004
    ..It will also serve as an example for similar microscope-based screens addressing different biological questions...