Christian Drosten

Summary

Affiliation: University of Hamburg
Country: Germany

Publications

  1. pmc Viral load as predictor of Crimean-Congo hemorrhagic fever outcome
    Darja Duh
    Institute of Microbiology and Immunology, Ljubljana, Slovenia
    Emerg Infect Dis 13:1769-72. 2007
  2. pmc Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR
    Christian Drosten
    Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 40:2323-30. 2002
  3. pmc Distant relatives of severe acute respiratory syndrome coronavirus and close relatives of human coronavirus 229E in bats, Ghana
    Susanne Pfefferle
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Emerg Infect Dis 15:1377-84. 2009
  4. pmc Prevalence, types, and RNA concentrations of human parechoviruses, including a sixth parechovirus type, in stool samples from patients with acute enteritis
    Sigrid Baumgarte
    Laboratory of Virology, Department of Microbiological Consumer Protection, Institute of Hygiene and the Environment, Hamburg, Germany
    J Clin Microbiol 46:242-8. 2008
  5. ncbi request reprint Rapid detection and differentiation of human pathogenic orthopox viruses by a fluorescence resonance energy transfer real-time PCR assay
    Marcus Panning
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Clin Chem 50:702-8. 2004
  6. pmc Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full genome
    Luciano Kleber de Souza Luna
    Clinical Virology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Virol J 5:26. 2008
  7. doi request reprint No evidence of chikungunya virus and antibodies shortly before the outbreak on Sri Lanka
    Marcus Panning
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Med Microbiol Immunol 198:103-6. 2009
  8. pmc Evaluation of advanced reverse transcription-PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome-associated coronavirus
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 42:2043-7. 2004
  9. pmc A novel diagnostic target in the hepatitis C virus genome
    Jan Felix Drexler
    Clinical Virology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS Med 6:e31. 2009
  10. pmc Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo
    Susanne Pfefferle
    Clinical Virology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Virol J 6:131. 2009

Detail Information

Publications67

  1. pmc Viral load as predictor of Crimean-Congo hemorrhagic fever outcome
    Darja Duh
    Institute of Microbiology and Immunology, Ljubljana, Slovenia
    Emerg Infect Dis 13:1769-72. 2007
    ..Viral load correlated with clinical disease and antibodies and could be used as a predictor of disease outcome...
  2. pmc Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR
    Christian Drosten
    Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 40:2323-30. 2002
    ..6 to 16 RNA copies per assay). The suitability of the assays was exemplified by detection and quantification of viral RNA in serum samples of VHF patients...
  3. pmc Distant relatives of severe acute respiratory syndrome coronavirus and close relatives of human coronavirus 229E in bats, Ghana
    Susanne Pfefferle
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Emerg Infect Dis 15:1377-84. 2009
    ..The most recent common ancestor of hCoV-229E and GhanaBt-CoVGrp1 existed in approximately 1686-1800 ad. The GhanaBt-CoVGrp2 shared an old ancestor (approximately 2,400 years) with the severe acute respiratory syndrome-like group of CoV...
  4. pmc Prevalence, types, and RNA concentrations of human parechoviruses, including a sixth parechovirus type, in stool samples from patients with acute enteritis
    Sigrid Baumgarte
    Laboratory of Virology, Department of Microbiological Consumer Protection, Institute of Hygiene and the Environment, Hamburg, Germany
    J Clin Microbiol 46:242-8. 2008
    ..Full genome analysis of the two viruses revealed recombination between HPeV1 and -3 in one and HPeV6 and -1 in another. HPeV seems relevant in children <2 years and specific RT-PCR for HPeV should be included in enteritis screening...
  5. ncbi request reprint Rapid detection and differentiation of human pathogenic orthopox viruses by a fluorescence resonance energy transfer real-time PCR assay
    Marcus Panning
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Clin Chem 50:702-8. 2004
    ..PCR is a powerful tool to detect and differentiate orthopox viruses, and real-time PCR has the further advantages of rapid turnaround time, low risk of contamination, capability of strain differentiation, and use of multiplexed probes...
  6. pmc Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full genome
    Luciano Kleber de Souza Luna
    Clinical Virology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Virol J 5:26. 2008
    ..In this study we used VIDISCA, a virus identification method which has not yet been widely applied, on such an untyped virus isolate...
  7. doi request reprint No evidence of chikungunya virus and antibodies shortly before the outbreak on Sri Lanka
    Marcus Panning
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Med Microbiol Immunol 198:103-6. 2009
    ..However, no evidence of acute or previous chikungunya infection could be demonstrated in the same cohort. This study gives formal evidence that the absence of human population immunity correlated with a large chikungunya epidemic...
  8. pmc Evaluation of advanced reverse transcription-PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome-associated coronavirus
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 42:2043-7. 2004
    ..Methods for the routine sampling of sputum without infection risk are needed to improve SARS RT-PCR...
  9. pmc A novel diagnostic target in the hepatitis C virus genome
    Jan Felix Drexler
    Clinical Virology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS Med 6:e31. 2009
    ..Non-western HCV genotypes have been under-represented in evaluation studies. An alternative diagnostic target region within the HCV genome could facilitate a new generation of assays...
  10. pmc Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo
    Susanne Pfefferle
    Clinical Virology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Virol J 6:131. 2009
    ..Because attenuation was focused on the early phase of infection in-vivo, ORF 7b might have contributed to the delayed accumulation of virus in patients that was suggested to have limited the spread of the SARS epidemic...
  11. pmc The SARS-coronavirus-host interactome: identification of cyclophilins as target for pan-coronavirus inhibitors
    Susanne Pfefferle
    Bernhard Nocht Institute, Hamburg, Germany
    PLoS Pathog 7:e1002331. 2011
    ..Non-immunosuppressive derivatives of CspA might serve as broad-range CoV inhibitors applicable against emerging CoVs as well as ubiquitous pathogens of humans and livestock...
  12. pmc Coordinated implementation of chikungunya virus reverse transcription-PCR
    Marcus Panning
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Emerg Infect Dis 15:469-71. 2009
    ..An international proficiency study with 31 participants demonstrated that ad hoc implementation of molecular diagnostics was feasible and successful...
  13. ncbi request reprint Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro
    Stephan Gunther
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    Antiviral Res 63:209-15. 2004
    ..The IC(50) values of the most active compounds ranged from 5 to 21 micrograms/ml. The compounds also inhibited replication of SARS coronavirus and Ebola virus in analogous assays, although to a lesser extent than Lassa virus...
  14. pmc Inhibition of different Lassa virus strains by alpha and gamma interferons and comparison with a less pathogenic arenavirus
    Marcel Asper
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, 20359 Hamburg, Germany
    J Virol 78:3162-9. 2004
    ..Both cytokines inhibit replication which is relevant for the design of antiviral strategies against Lassa fever with the aim of enhancing the IFN response...
  15. pmc Chikungunya fever in travelers returning to Europe from the Indian Ocean region, 2006
    Marcus Panning
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Emerg Infect Dis 14:416-22. 2008
    ..Soon after onset of symptoms, patients had IgG and IgM and high viral loads (some >10(9) copies/mL plasma). These data will help healthcare providers select diagnostic tests for returning travelers...
  16. ncbi request reprint Detection of Mycobacterium tuberculosis by real-time PCR using pan-mycobacterial primers and a pair of fluorescence resonance energy transfer probes specific for the M. tuberculosis complex
    Christian Drosten
    Bernhard Nocht Institute of Tropical Medicine, National Reference Centre for Tropical Infections, 20359 Hamburg, Germany
    Clin Chem 49:1659-61. 2003
  17. ncbi request reprint Establishment of conventional and fluorescence resonance energy transfer-based real-time PCR assays for detection of pathogenic New World arenaviruses
    Simon Vieth
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Clin Virol 32:229-35. 2005
    ..Four of the viruses, namely Junin, Guanarito, Machupo, and Sabia, belong to clade B of New World arenaviruses that also comprises the nonpathogenic viruses Tacaribe, Cupixi, and Amapari...
  18. ncbi request reprint Ultrasensitive monitoring of HIV-1 viral load by a low-cost real-time reverse transcription-PCR assay with internal control for the 5' long terminal repeat domain
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Clin Chem 52:1258-66. 2006
    ..In some countries, monitoring of antiretroviral therapy is now more expensive than treatment itself. In addition, some commercial assays have shown shortcomings in quantifying rare genotypes...
  19. pmc Orientia tsutsugamushi bacteremia and cytokine levels in Vietnamese scrub typhus patients
    Stefanie Kramme
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 47:586-9. 2009
    ..They may thus be induced by different constituents of O. tsutsugamushi. As a future prospect in a clinical diagnostic laboratory, quantitative real-time PCR may serve as a reliable tool to monitor therapy and to detect treatment failure...
  20. ncbi request reprint Molecular diagnostics of viral hemorrhagic fevers
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    Antiviral Res 57:61-87. 2003
    ..The third part is focussed on important differential diagnoses of VHF...
  21. pmc Diagnosing schistosomiasis by detection of cell-free parasite DNA in human plasma
    Dominic Wichmann
    Sektion Infektiologie und Tropenmedizin, I Medizinische Klinik, Universitätsklinikum Eppendorf, Hamburg, Germany
    PLoS Negl Trop Dis 3:e422. 2009
    ..Cell-free parasite DNA (CFPD) was detected in plasma by PCR...
  22. ncbi request reprint Prevalence of hepatitis B virus DNA in anti-HBc-positive/HBsAg-negative sera correlates with HCV but not HIV serostatus
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Clin Virol 29:59-68. 2004
    ..Hepatitis B virus (HBV) DNA often remains detectable in serum despite clinical recovery and loss of HBsAg...
  23. ncbi request reprint Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases, Hamburg, Germany
    N Engl J Med 348:1967-76. 2003
    ..The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent...
  24. pmc Comparative analysis of rabies virus reverse transcription-PCR and virus isolation using samples from a patient infected with rabies virus
    Marcus Panning
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 48:2960-2. 2010
    ..For the first time, a direct comparison of virus isolation with quantitative real-time reverse transcription (RT)-PCR on human rabies samples was conducted. RT-PCR was found to be more sensitive than virus isolation...
  25. pmc Xenotropic murine leukemia virus-related gammaretrovirus in respiratory tract
    Nicole Fischer
    Institute for Medical Microbiology and Virology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Emerg Infect Dis 16:1000-2. 2010
    ..XMRV-specific sequences were detected in 2%-3% of samples from 168 immunocompetent carriers and approximately 10% of samples from 161 immunocompromised patients...
  26. ncbi request reprint Monitoring of clinical and laboratory data in two cases of imported Lassa fever
    Herbert Schmitz
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    Microbes Infect 4:43-50. 2002
    ..These data suggest that a high virus load and high levels of pro-inflammatory cytokines in the late stage of Lassa fever play an important role in the pathogenesis of hemorrhage, multi-organ failure, and shock in Lassa fever...
  27. doi request reprint Hepatitis E virus infections in HIV-infected patients in Ghana and Cameroon
    Torsten Feldt
    Clinical Research Unit, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    J Clin Virol 58:18-23. 2013
    ..Only few data are available for sub-Saharan Africa, where HIV and HEV are highly co-endemic, and where liver pathology is common in HIV-infected individuals...
  28. pmc SARS molecular detection external quality assurance
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Emerg Infect Dis 10:2200-3. 2004
    ..Of 58 participants, 51 correctly detected virus in all samples > or =9,400 RNA copies per milliliter and none in negative samples. Commercial test kits significantly improved the outcome...
  29. ncbi request reprint Spectrum of viruses and atypical bacteria in intercontinental air travelers with symptoms of acute respiratory infection
    Luciano Kleber de Souza Luna
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Infect Dis 195:675-9. 2007
    ..To our knowledge, these are the first specific baseline data for the mentioned agents in the context of air travel...
  30. ncbi request reprint Detection and quantification of Mycobacterium leprae in tissue samples by real-time PCR
    Stefanie Kramme
    National Reference Center for Tropical Infections, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    Med Microbiol Immunol 193:189-93. 2004
    ..On the other hand, as still no culture system exists for M. leprae, the assay appears to be a robust tool for detection of the bacterium in selected clinical situations, as well as for quantitation in experimental settings...
  31. pmc Detection, differentiation, and quantitation of pathogenic leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay
    Alexandra Schulz
    National Reference Center for Tropical Infections Leishmaniasis Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 41:1529-35. 2003
    ..89%). Therefore, despite its superior analytical performance, care must be taken when real-time PCR is utilized for therapy monitoring...
  32. pmc Henipavirus RNA in African bats
    Jan Felix Drexler
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS ONE 4:e6367. 2009
    ..These bats do not occur in continental Africa, but a whole range of other fruit bats is encountered. One of the most abundant is Eidolon helvum, the African Straw-coloured fruit bat...
  33. pmc Virus detection and monitoring of viral load in Crimean-Congo hemorrhagic fever virus patients
    Roman Wölfel
    Bundeswehr Institute of Microbiology, Munich, Germany
    Emerg Infect Dis 13:1097-100. 2007
    ..Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection...
  34. pmc Viral load and Crimean-Congo hemorrhagic fever
    Anna Papa
    Emerg Infect Dis 13:805-6. 2007
  35. ncbi request reprint Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories
    Marcus Panning
    Bernhard Nocht Institute for Tropical Medicine, Hamburg 20359, Germany
    J Infect Dis 196:S199-204. 2007
    ..The assay facilitates reliable detection or exclusion screening of filovirus infections...
  36. ncbi request reprint RT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene
    Simon Vieth
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    Trans R Soc Trop Med Hyg 101:1253-64. 2007
    ....
  37. ncbi request reprint Infection of SCID mice with Montana Myotis leukoencephalitis virus as a model for flavivirus encephalitis
    Nathalie Charlier
    Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    J Gen Virol 83:1887-96. 2002
    ..This validates the MMLV model for use in antiviral drug studies. The MMLV SCID model may, therefore, be attractive for the study of chemoprophylactic or chemotherapeutic strategies against flavivirus infections causing encephalitis...
  38. ncbi request reprint Imported Lassa fever in Germany: surveillance and management of contact persons
    Walter H Haas
    Robert Koch Institute, Berlin, Germany
    Clin Infect Dis 36:1254-8. 2003
    ..The study indicates a low risk of transmission during the initial phase of symptomatic Lassa fever, even with high-risk exposures. The risk may increase with progression of disease and increasing virus load...
  39. pmc Lassa fever, Nigeria, 2003 and 2004
    Sunday Aremu Omilabu
    Emerg Infect Dis 11:1642-4. 2005
  40. pmc Interferons, interferon inducers, and interferon-ribavirin in treatment of flavivirus-induced encephalitis in mice
    Pieter Leyssen
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Antimicrob Agents Chemother 47:777-82. 2003
    ..However, ribavirin did not enhance the inhibitory effect of interferon therapy in this animal model for flavivirus infections...
  41. pmc Rates of and reasons for failure of commercial human immunodeficiency virus type 1 viral load assays in Brazil
    Jan Felix Drexler
    Bernhard Nocht Institute for Tropical Medicine, Clinical Virology Section, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    J Clin Microbiol 45:2061-3. 2007
    ..Failure rates for Bayer Versant bDNA 3.0, Roche Amplicor Monitor v1.5, and bioMerieux NucliSens QT were 0.68, 0.47, and 4.33%, respectively. NucliSens may be inadequate for use in Brazil...
  42. pmc Exchanging the yellow fever virus envelope proteins with Modoc virus prM and E proteins results in a chimeric virus that is neuroinvasive in SCID mice
    Nathalie Charlier
    Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    J Virol 78:7418-26. 2004
    ..The observations provide compelling evidence that the determinants of neuroinvasiveness of flaviviruses are entirely located in the envelope proteins prM and E...
  43. ncbi request reprint Analysis of two imported cases of yellow fever infection from Ivory Coast and The Gambia to Germany and Belgium
    Hi Gung Bae
    Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
    J Clin Virol 33:274-80. 2005
    ..Non-vaccinated travelers take a high risk without the effective protection of YFV 17D vaccination...
  44. pmc Cinanserin is an inhibitor of the 3C-like proteinase of severe acute respiratory syndrome coronavirus and strongly reduces virus replication in vitro
    Lili Chen
    Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
    J Virol 79:7095-103. 2005
    ..These findings demonstrate that the old drug cinanserin is an inhibitor of SARS-CoV replication, acting most likely via inhibition of the 3CL proteinase...
  45. pmc HIV-1 viral load assays for resource-limited settings: clades matter
    Wolfgang Preiser
    PLoS Med 3:e538; author reply e550. 2006
  46. ncbi request reprint Reverse ELISA for IgG and IgM antibodies to detect Lassa virus infections in Africa
    Petra Emmerich
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, D 20356 Hamburg, Germany
    J Clin Virol 37:277-81. 2006
    ..Anti-Lassa antibodies are detected by indirect immunofluorescence assay (IFA) or by enzyme-immunoassay (ELISA). Both methods have problems to detect low amounts of specific antibodies...
  47. pmc False-negative results of PCR assay with plasma of patients with severe viral hemorrhagic fever
    Christian Drosten
    J Clin Microbiol 40:4394-5. 2002
  48. ncbi request reprint External quality control assessment in PCR diagnostics of dengue virus infections
    Karin Lemmer
    Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    J Clin Virol 30:291-6. 2004
    ..Nucleic acid amplification techniques (NAT) are now frequently used for rapid diagnosis of imported viral diseases. Different PCR systems are available...
  49. pmc Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray
    Luciano Kleber de Souza Luna
    Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    J Clin Microbiol 45:1049-52. 2007
    ..In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs...
  50. pmc Topographic and quantitative display of integrated human immunodeficiency virus-1 provirus DNA in human lymph nodes by real-time polymerase chain reaction
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Virology Molecular Diagnostics, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    J Mol Diagn 7:219-25. 2005
    ..1% of all follicular cells). The results of this method suggest that the high density of provirus-containing cells in follicles may be important for the persistence of proviral DNA in infected persons...
  51. pmc Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein
    Tsanan Giroglou
    Georg Speyer Haus, Institute for Biomedical Research, Frankfurt University Medical School, Frankfurt, Germany
    J Virol 78:9007-15. 2004
    ....
  52. ncbi request reprint NAT for HBV and anti-HBc testing increase blood safety
    W Kurt Roth
    Institute of Transfusion Medicine and Immunohematology German Red Cross, Hesse, Frankfurt
    Transfusion 42:869-75. 2002
    ..Testing was successively extended to customer transfusion services with a total of 1,300,000 samples tested per year...
  53. ncbi request reprint Yield of HCV and HIV-1 NAT after screening of 3.6 million blood donations in central Europe
    W Kurt Roth
    Institute of Transfusion Medicine and Immunohematology German Red Cross, Hesse, Frankfurt
    Transfusion 42:862-8. 2002
    ..The yield of NAT after testing more than 3.6 million donations in central Europe is reported...
  54. pmc Crimean-Congo hemorrhagic fever in Kosovo
    Christian Drosten
    J Clin Microbiol 40:1122-3. 2002
  55. pmc Real-time PCR system for detection of orthopoxviruses and simultaneous identification of smallpox virus
    Victoria A Olson
    Poxvirus Section, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 42:1940-6. 2004
    ..The assay proved to be a reliable technique for the detection of orthopoxviruses, with the advantage that it can simultaneously identify variola virus...
  56. pmc First international quality assurance study on the rapid detection of viral agents of bioterrorism
    Matthias Niedrig
    Robert Koch Institute, Berlin, Germany
    J Clin Microbiol 42:1753-5. 2004
    ..Sensitivity levels were not significantly different between viruses. False-negative results were attributable to a lack of sensitivity...
  57. ncbi request reprint Clinical and laboratory findings on the first imported case of Murray Valley encephalitis in Europe
    August Stich
    Medical Mission Hospital, Department of Tropical Medicine, D 97074 Wurzburg, Germany
    Clin Infect Dis 37:e19-21. 2003
    ..This emphasizes the need for continuous surveillance in areas of endemicity and appropriate protection when traveling through regions in which the MVE virus is endemic...
  58. ncbi request reprint Chikungunya fever in travelers: clinical presentation and course
    Winfried Taubitz
    Department of Infectious Diseases and Tropical Medicine, University Munich, Germany
    Clin Infect Dis 45:e1-4. 2007
    ..5 million patients, including travelers who have visited these areas...
  59. pmc Coronaviruses in children, Greece
    Anna Papa
    Emerg Infect Dis 13:947-9. 2007
  60. ncbi request reprint First international proficiency study on West Nile virus molecular detection
    Matthias Niedrig
    Robert Koch Institute, Berlin, Germany
    Clin Chem 52:1851-4. 2006
    ..West Nile virus (WNV) molecular detection is being conducted by a growing number of laboratories, but the degree of proficiency may vary between them. External quality control is needed...
  61. ncbi request reprint NAT screening of blood donors for severe acute respiratory syndrome coronavirus can potentially prevent transfusion associated transmissions
    Michael Schmidt
    Institute of Transfusion Medicine and Immunohematology, German Red Cross, Johan Wolfgang Goethe University, Frankfurt, Germany
    Transfusion 44:470-5. 2004
    ..The severe acute respiratory syndrome (SARS) was first described in February 2003. Close contact with symptomatic patients appears to be the main route of transmission, whereas blood transfusion transmission could not be ruled out...
  62. pmc MassTag polymerase chain reaction for differential diagnosis of viral hemorrhagic fever
    Gustavo Palacios
    Columbia University, New York, New York 10032, USA
    Emerg Infect Dis 12:692-5. 2006
    ..We describe a diagnostic system for rapid, multiplex polymerase chain reaction identification of 10 different causes of viral hemorrhagic fevers...
  63. ncbi request reprint Impact of direct virus-induced neuronal dysfunction and immunological damage on the progression of flavivirus (Modoc) encephalitis in a murine model
    Pieter Leyssen
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium
    J Neurovirol 9:69-78. 2003
    ....
  64. pmc Coronavirus antibodies in African bat species
    Marcel A Muller
    Robert Koch Institut, Center for Biological Safety, Berlin, Germany
    Emerg Infect Dis 13:1367-70. 2007
    ..We detected antibody reactive with SARS-CoV antigen in 47 (6.7%) of 705 bat serum specimens comprising 26 species collected in Africa; thus, African bats may harbor agents related to putative group 4 CoV...
  65. ncbi request reprint Detection of Ebola virus in oral fluid specimens during outbreaks of Ebola virus hemorrhagic fever in the Republic of Congo
    Pierre Formenty
    World Health Organization, Department of Communicable Diseases Surveillance and Response, Geneva, Switzerland
    Clin Infect Dis 42:1521-6. 2006
    ..During the 2 EVHF outbreaks in the Republic of Congo in 2003, we assessed the use of oral fluid specimens versus serum samples for laboratory confirmation of cases of EVHF...
  66. doi request reprint Evaluation of the first commercial chikungunya virus indirect immunofluorescence test
    Nadine Litzba
    Centre for Biological Safety ZBS 1, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    J Virol Methods 149:175-9. 2008
    ..3% and a sensitivity of 96.9%. The specificity and sensitivity for the IgG IIFT are 100.0% and 95.4%, respectively. This commercial IIFT is a valuable tool for the diagnosis of CHIKV infections and antibody seroprevalence studies...
  67. ncbi request reprint Characterization of a novel coronavirus associated with severe acute respiratory syndrome
    Paul A Rota
    National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Science 300:1394-9. 2003
    ..Phylogenetic analyses and sequence comparisons showed that SARS-CoV is not closely related to any of the previously characterized coronaviruses...