Research Topics
Species | Thomas CremerSummaryAffiliation: University of Munich Country: Germany Publications
| Collaborators
|
Detail Information
Publications
Rise, fall and resurrection of chromosome territories: a historical perspective. Part I. The rise of chromosome territoriesThomas Cremer
LMU Biozentrum, Antropology and Human Genetics, Department of Biology II, Martinsried, Germany
Eur J Histochem 50:161-76. 2006..In part II (next volume) we review the abandonment of the chromosome territory concept during the 1950th to 1980th and the compelling evidence, which led to its resurrection during the 1970th to 1980th...- Chromosome territories, nuclear architecture and gene regulation in mammalian cellsT Cremer
Institute of Anthropology and Human Genetics, Ludwig Maximilians University, Richard Wagner Strasse 10, D 80333 Munich, Germany
Nat Rev Genet 2:292-301. 2001..This view is consistent with a topological model for gene regulation... - Evolutionarily conserved, cell type and species-specific higher order chromatin arrangements in interphase nuclei of primatesMichaela Neusser
Department Biology II, Human Genetics, Ludwig Maximilians University, Grosshaderner Strasse 2, 82152 Planegg Martinsried, Germany
Chromosoma 116:307-20. 2007..Our results indicate that nonrandom breakage and rejoining of preferentially gene-dense chromosomes or chromosome segments may have occurred during evolution... 
Nuclear architecture in developmental biology and cell specialisationThomas Cremer
LMU BioCenter, Grosshadernerstr 2, D 82152 Martinsried, Germany
Reprod Fertil Dev 23:94-106. 2011....
Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettesAndreas Bolzer
Department of Biology II, Anthropology and Human Genetics, Ludwig Maximilians University, Munich, Germany
PLoS Biol 3:e157. 2005..Chromatin domains, which are gene-poor, form a layer beneath the nuclear envelope, while gene-dense chromatin is enriched in the nuclear interior. We discuss the possible functional implications of this finding...- Establishment and mitotic stability of an extra-chromosomal mammalian repliconIsa M Stehle
Institute of Cell Biology, University Witten Herdecke, Witten, Germany
BMC Cell Biol 8:33. 2007..In order to uncouple local and long-range epigenetic features we used an extra-chromosomal replicon to study the requirements for replication and segregation and compared its behavior to that of its integrated counterpart... - Chromosome territories--a functional nuclear landscapeThomas Cremer
Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, D 82152, Martinsried, Germany
Curr Opin Cell Biol 18:307-16. 2006.... - Rise, fall and resurrection of chromosome territories: a historical perspective. Part II. Fall and resurrection of chromosome territories during the 1950s to 1980s. Part III. Chromosome territories and the functional nuclear architecture: experiments and T Cremer
Department of Biology II, 82152 Martinsried, Germany
Eur J Histochem 50:223-72. 2006.... - Higher order chromatin architecture in the cell nucleus: on the way from structure to functionThomas Cremer
Department Biologie II, Ludwig Maximilians University, Richard Wagner Street 10 I, 80333 Munich, Germany
Biol Cell 96:555-67. 2004..In addition we discuss the advantages and drawbacks of experimental approaches currently used to study nuclear architecture and function in fixed and living cells... 
Chromosome territoriesThomas Cremer
Biozentrum, Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, 82152 Martinsried, Germany
Cold Spring Harb Perspect Biol 2:a003889. 2010..The article concludes with a discussion of open questions and new experimental strategies to answer them...- Replication-timing-correlated spatial chromatin arrangements in cancer and in primate interphase nucleiFlorian Grasser
Department of Biology II, Human Genetics, Ludwig Maximilians University Munich, Planegg Martinsreid, Germany
J Cell Sci 121:1876-86. 2008.... - Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networksHeiner Albiez
Department of Biology II, LMU Biozentrum, Grosshaderner Strasse 2, 82152, Planegg-Martinsried, Germany
Chromosome Res 14:707-33. 2006..These experiments, together with electron microscopic observations, demonstrate the existence of the IC as a dynamic, structurally distinct nuclear compartment, which is functionally linked with the chromatin compartment... - Common themes and cell type specific variations of higher order chromatin arrangements in the mouseRobert Mayer
Department Biologie II, Ludwig Maximilians Universitat Munchen, Grosshaderner Str 2, 82152 Planegg Martinsried, Germany
BMC Cell Biol 6:44. 2005.... - Differences in centromere positioning of cycling and postmitotic human cell typesIrina Solovei
Department of Biology II, Humangenetik, Ludwig Maximillians University LMU, Richard Wagner Str 10, 80333, Munich, Germany
Chromosoma 112:410-23. 2004..This asymmetrical distribution of centromeres is considered to be a consequence of chromosome arrangement in anaphase rosettes... - Multicolor 3D fluorescence in situ hybridization for imaging interphase chromosomesMarion Cremer
Department of Biology II, Ludwig Maximilians University Biozentrum, Planegg Martinsried, Germany
Methods Mol Biol 463:205-39. 2008.... - Spatial preservation of nuclear chromatin architecture during three-dimensional fluorescence in situ hybridization (3D-FISH)Irina Solovei
Department of Biology II, Ludwig Maximilians University of Munich, Germany
Exp Cell Res 276:10-23. 2002..However, important ultrastructural details of the chromatin architecture are destroyed by the heat denaturation step, thus putting a limit to the usefulness of 3D-FISH analyses at nanometer scales... - Remodeling of nuclear architecture by the thiodioxoxpiperazine metabolite chaetocinDoris Illner
Biozentrum, Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, 82152 Martinsried, Germany
Exp Cell Res 316:1662-80. 2010..The mechanisms involved in CICC formation remain to be fully explored... - The architecture of chicken chromosome territories changes during differentiationSonja Stadler
Department Biologie II, Biozentrum der Ludwig Maximilians Universität München, Planegg Martinsried, Germany
BMC Cell Biol 5:44. 2004..Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown... - Histone lysine methylation patterns in human cell types are arranged in distinct three-dimensional nuclear zonesRoman Zinner
Anthropology and Human Genetics, Department of Biology II, Ludwig-Maximilians-University, Grosshadernerstrasse 2, D-82152, Martinsried, Germany
Histochem Cell Biol 125:3-19. 2006..Finally we demonstrated by simultaneous visualization of different histone lysine methylation sites that methylation patterns are organized in distinct nuclear zones with little apparent intermingling... - Maintenance of imprinting and nuclear architecture in cycling cellsKathrin Teller
Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, 82152 Planegg Martinsried, Germany
Proc Natl Acad Sci U S A 104:14970-5. 2007.... - Radial chromatin positioning is shaped by local gene density, not by gene expressionKatrin Küpper
Department of Biology II, Anthropology and Human Genetics, Ludwig Maximilians University, Munich, Germany
Chromosoma 116:285-306. 2007..5 often loops out from the territory surface, gene-dense and highly expressed sequences were not generally found preferentially at the CT surface as previously suggested... - Changes of higher order chromatin arrangements during major genome activation in bovine preimplantation embryosDaniela Koehler
Division of Anthropology and Human Genetics, Biocenter, LMU Munich, Grosshadernerstr 2, D 82152 Martinsried, Germany
Exp Cell Res 315:2053-63. 2009..Since major genome activation in bovine embryos occurs at the 8- to 16-cell stage, our findings demonstrate a temporal correlation between transcriptional activation and a major rearrangement of chromatin topography in blastomere nuclei... - Double-strand break-induced transcriptional silencing is associated with loss of tri-methylation at H3K4Doris M Seiler
Department of Radiation Oncology, University Hospital of Munich, Schillerstr 42, 80336, Munich, Germany
Chromosome Res 19:883-99. 2011..Since no accumulation of the repressive mark H3K9me2 was found at damaged sites, we suggest that DSB-induced transcriptional silencing resembles polycomb-mediated silencing rather than heterochromatic silencing... - Biochemistry meets nuclear architecture: multicolor immuno-FISH for co-localization analysis of chromosome segments and differentially expressed gene loci with various histone methylationsRoman Zinner
Anthropology and Human Genetics, Department of Biology II, Ludwig-Maximilians-University, Grosshadernerstrasse 2, D-82152 Martinsried, Germany
Adv Enzyme Regul 47:223-41. 2007 - Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolutionIrina Solovei
Division of Anthropology and Human Genetics, Biocenter, Ludwig Maximilians University Munich, Grosshadernerstrasse 2, 82152 Planegg Martinsried, Germany
Cell 137:356-68. 2009..Comparison of the two patterns suggests that the conventional architecture prevails in eukaryotic nuclei because it results in more flexible chromosome arrangements, facilitating positional regulation of nuclear functions... - The potential of 3D-FISH and super-resolution structured illumination microscopy for studies of 3D nuclear architecture: 3D structured illumination microscopy of defined chromosomal structures visualized by 3D (immuno)-FISH opens new perspectives for studYolanda Markaki
Biocenter, Department Biology II, Ludwig Maximilians University LMU, Martinsried, Germany
Bioessays 34:412-26. 2012.... - A top-down analysis of Xa- and Xi-territories reveals differences of higher order structure at ≥ 20 Mb genomic length scalesKathrin Teller
Biocenter, Department Biology II, Ludwig Maximilians University LMU, Martinsried, Germany
Nucleus 2:465-77. 2011..In contrast to a previous report, genes subject to inactivation as well as escaping from inactivation were not excluded from the interior of the Barr body... - Evolutionary conservation of chromosome territory arrangements in cell nuclei from higher primatesHideyuki Tanabe
Department of Biology II-Human Genetics, University of Munich, Richard Wagner Strasse 10, , Germany
Proc Natl Acad Sci U S A 99:4424-9. 2002..The evolutionarily conserved positioning of homologous chromosomes or chromosome segments in related species supports evidence for a functionally relevant higher-order chromatin arrangement that is correlated with gene-density... - Inheritance of gene density-related higher order chromatin arrangements in normal and tumor cell nucleiMarion Cremer
Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany
J Cell Biol 162:809-20. 2003..e., a CT #18 located more internally than a CT #19. This observation emphasizes a partial loss of radial chromatin order in tumor cell nuclei... - Three-dimensional arrangements of centromeres and telomeres in nuclei of human and murine lymphocytesClaudia Weierich
Department of Biology II, Human Genetics, Ludwig Maximillians University (LMU, Richard Wagner Str. 10, 80333 Munich, Germany
Chromosome Res 11:485-502. 2003..While the centromere of the active X often participated in the formation of centromere clusters, such a participation was never observed for the centromere of the inactive X... - Replication labeling patterns and chromosome territories typical of mammalian nuclei are conserved in the early metazoan HydraOlga Alexandrova
Department of Biology II, Cell and Developmental Biology, Ludwig Maximilians University, Munich, Germany
Chromosoma 112:190-200. 2003..Our results indicate the conservation of fundamental features of higher order chromatin arrangements throughout the evolution of metazoan animals and suggest the existence of conserved mechanism(s) controlling this architecture... - Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensionsChristian Lanctôt
Department Biologie II, Ludwig Maximilians Universitat, Grosshadernerstr 2, Planegg Martinsried, Germany
Nat Rev Genet 8:104-15. 2007..Understanding how the dynamic nature of the positioning of genetic material in the nuclear space and the higher-order architecture of the nucleus are integrated is therefore essential to our overall understanding of gene regulation... - Methyl CpG-binding proteins induce large-scale chromatin reorganization during terminal differentiationAlessandro Brero
Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
J Cell Biol 169:733-43. 2005..This MeCP2- and MBD2-mediated chromatin reorganization may thus represent a molecular link between nuclear genome topology and the epigenetic maintenance of cellular differentiation... - Positioning of the mouse Hox gene clusters in the nuclei of developing embryos and differentiating embryoid bodiesChristian Lanctôt
Department Biology II, Ludwig Maximilians University Munich, Grosshadernerstr 2, Planegg Martinsried, 82152, Germany
Exp Cell Res 313:1449-59. 2007.... - 4D chromatin dynamics in cycling cells: Theodor Boveri's hypotheses revisitedHilmar Strickfaden
Department Biology II Anthropology and Human Genetics, LMU Biozentrum, Martinsried, Germany
Nucleus 1:284-97. 2010..A new model of chromatin dynamics is proposed. It suggests that long-range DNA-DNA interactions in cell nuclei may depend on a combination of rotational CT movements and locally constrained chromatin movements... - Functional nuclear architecture studied by microscopy: present and futureJacques Rouquette
Biocenter, Ludwig Maximilians University LMU, Martinsried, Germany
Int Rev Cell Mol Biol 282:1-90. 2010..This will make possible future analyses of cell type- and species-specific differences of nuclear architecture in more detail and to put different models to critical tests... - Revealing the high-resolution three-dimensional network of chromatin and interchromatin space: a novel electron-microscopic approach to reconstructing nuclear architectureJacques Rouquette
Biocenter, Ludwig Maximilians University LMU, Grosshaderner Str 2, D 82152 Martinsried, Germany
Chromosome Res 17:801-10. 2009..Our results show the importance of the contribution of such reconstruction techniques to our understanding of the nuclear architecture... - The radial nuclear positioning of genes correlates with features of megabase-sized chromatin domainsAlexandra C Kölbl
Department Biologie II, Ludwig Maximilians Universitat Munchen, 82152, Planegg, Martinsried, Germany
Chromosome Res 20:735-52. 2012..Our results strongly support the hypothesis that knowledge of the local chromatin environment is essential to predict the radial nuclear position of a gene... - Non-random radial arrangements of interphase chromosome territories: evolutionary considerations and functional implicationsHideyuki Tanabe
Cell Bank Laboratory, Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo, Japan
Mutat Res 504:37-45. 2002.... - Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stagesJoachim Walter
Dept. Biologie II, , LMU, Richard-Wagner-Strasse 10/I, , Germany
J Cell Biol 160:685-97. 2003..The variability of CT neighborhoods during clonal growth was further confirmed by chromosome painting experiments... - Epigenomic differentiation in mouse preimplantation nuclei of biparental, parthenote and cloned embryosValeria Merico
Dipartimento di Biologia Animale, Laboratorio di Biologia dello Sviluppo, Piazza Botta 9, Universita degli Studi di Pavia, 27100, Pavia, Italy
Chromosome Res 15:341-60. 2007..This finding suggests that the epigenome of the three types of embryos partially acts as a constraint of the nuclear organization of the three nuclear subcompartments analysed... - Nuclear architecture: Is it important for genome function and can we prove it?Julio Mateos-Langerak
Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
J Cell Biochem 102:1067-75. 2007..Numerous observations indicate a tight correlation between genome activity and nuclear and large-scale chromatin structure. However, causal relationships are rare. Here we explore how these might be uncovered... - Exploiting nuclear duality of ciliates to analyse topological requirements for DNA replication and transcriptionJan Postberg
Institute of Cell Biology, University of Witten Herdecke, Stockumer Str 10, 58453 Witten, Germany
J Cell Sci 118:3973-83. 2005..Moreover nuclear duality inherent to ciliates with their germline micronucleus and their somatic macronucleus may eventually provide further insight into epigenetic regulation of transcription, replication and nuclear differentiation... - Light optical precision measurements of the active and inactive Prader-Willi syndrome imprinted regions in human cell nucleiJoachim Rauch
Kirchhoff Institute of Physics, University of Heidelberg, Im Neuenheimer Feld 227, D 69120 Heidelberg, Germany
Differentiation 76:66-82. 2008..The possibilities to achieve this goal are discussed... - Non-rigid registration of 3D multi-channel microscopy images of cell nucleiSiwei Yang
Biomedical Computer Vision Group, Dept Theoretical Bioinformatics, DKFZ Heidelberg, Germany
Med Image Comput Comput Assist Interv 9:907-14. 2006..We also describe an extension of our approach which is applied for the registration of 3D+t (4D) image series of moving cell nuclei...