Thomas Cremer

Summary

Affiliation: University of Munich
Country: Germany

Publications

  1. ncbi request reprint Rise, fall and resurrection of chromosome territories: a historical perspective. Part I. The rise of chromosome territories
    Thomas Cremer
    LMU Biozentrum, Antropology and Human Genetics, Department of Biology II, Martinsried, Germany
    Eur J Histochem 50:161-76. 2006
  2. ncbi request reprint Chromosome territories, nuclear architecture and gene regulation in mammalian cells
    T Cremer
    Institute of Anthropology and Human Genetics, Ludwig Maximilians University, Richard Wagner Strasse 10, D 80333 Munich, Germany
    Nat Rev Genet 2:292-301. 2001
  3. ncbi request reprint Evolutionarily conserved, cell type and species-specific higher order chromatin arrangements in interphase nuclei of primates
    Michaela Neusser
    Department Biology II, Human Genetics, Ludwig Maximilians University, Grosshaderner Strasse 2, 82152 Planegg Martinsried, Germany
    Chromosoma 116:307-20. 2007
  4. doi request reprint Nuclear architecture in developmental biology and cell specialisation
    Thomas Cremer
    LMU BioCenter, Grosshadernerstr 2, D 82152 Martinsried, Germany
    Reprod Fertil Dev 23:94-106. 2011
  5. pmc Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages
    Joachim Walter
    Dept Biologie II, Lehrstuhl für Anthropologie und Humangenetik, LMU, Richard Wagner Strasse 10 I, 80333 München, Germany
    J Cell Biol 160:685-97. 2003
  6. doi request reprint Recollections of a scientific journey published in human genetics: from chromosome territories to interphase cytogenetics and comparative genome hybridization
    Thomas Cremer
    LMU Biozentrum, Grosshadernerstr 2, Martinsried, Germany
    Hum Genet 133:403-16. 2014
  7. pmc Establishment and mitotic stability of an extra-chromosomal mammalian replicon
    Isa M Stehle
    Institute of Cell Biology, University Witten Herdecke, Witten, Germany
    BMC Cell Biol 8:33. 2007
  8. ncbi request reprint Higher order chromatin architecture in the cell nucleus: on the way from structure to function
    Thomas Cremer
    Department Biologie II, Ludwig Maximilians University, Richard Wagner Street 10 I, 80333 Munich, Germany
    Biol Cell 96:555-67. 2004
  9. ncbi request reprint Chromosome territories--a functional nuclear landscape
    Thomas Cremer
    Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, D 82152, Martinsried, Germany
    Curr Opin Cell Biol 18:307-16. 2006
  10. ncbi request reprint Rise, fall and resurrection of chromosome territories: a historical perspective. Part II. Fall and resurrection of chromosome territories during the 1950s to 1980s. Part III. Chromosome territories and the functional nuclear architecture: experiments and
    T Cremer
    Department of Biology II, 82152 Martinsried, Germany
    Eur J Histochem 50:223-72. 2006

Collaborators

Detail Information

Publications47

  1. ncbi request reprint Rise, fall and resurrection of chromosome territories: a historical perspective. Part I. The rise of chromosome territories
    Thomas Cremer
    LMU Biozentrum, Antropology and Human Genetics, Department of Biology II, Martinsried, Germany
    Eur J Histochem 50:161-76. 2006
    ..In part II (next volume) we review the abandonment of the chromosome territory concept during the 1950th to 1980th and the compelling evidence, which led to its resurrection during the 1970th to 1980th...
  2. ncbi request reprint Chromosome territories, nuclear architecture and gene regulation in mammalian cells
    T Cremer
    Institute of Anthropology and Human Genetics, Ludwig Maximilians University, Richard Wagner Strasse 10, D 80333 Munich, Germany
    Nat Rev Genet 2:292-301. 2001
    ..This view is consistent with a topological model for gene regulation...
  3. ncbi request reprint Evolutionarily conserved, cell type and species-specific higher order chromatin arrangements in interphase nuclei of primates
    Michaela Neusser
    Department Biology II, Human Genetics, Ludwig Maximilians University, Grosshaderner Strasse 2, 82152 Planegg Martinsried, Germany
    Chromosoma 116:307-20. 2007
    ..Our results indicate that nonrandom breakage and rejoining of preferentially gene-dense chromosomes or chromosome segments may have occurred during evolution...
  4. doi request reprint Nuclear architecture in developmental biology and cell specialisation
    Thomas Cremer
    LMU BioCenter, Grosshadernerstr 2, D 82152 Martinsried, Germany
    Reprod Fertil Dev 23:94-106. 2011
    ....
  5. pmc Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages
    Joachim Walter
    Dept Biologie II, Lehrstuhl für Anthropologie und Humangenetik, LMU, Richard Wagner Strasse 10 I, 80333 München, Germany
    J Cell Biol 160:685-97. 2003
    ..The variability of CT neighborhoods during clonal growth was further confirmed by chromosome painting experiments...
  6. doi request reprint Recollections of a scientific journey published in human genetics: from chromosome territories to interphase cytogenetics and comparative genome hybridization
    Thomas Cremer
    LMU Biozentrum, Grosshadernerstr 2, Martinsried, Germany
    Hum Genet 133:403-16. 2014
    ..We conclude with a perspective on current developments and goals of molecular cytogenetics. ..
  7. pmc Establishment and mitotic stability of an extra-chromosomal mammalian replicon
    Isa M Stehle
    Institute of Cell Biology, University Witten Herdecke, Witten, Germany
    BMC Cell Biol 8:33. 2007
    ..In order to uncouple local and long-range epigenetic features we used an extra-chromosomal replicon to study the requirements for replication and segregation and compared its behavior to that of its integrated counterpart...
  8. ncbi request reprint Higher order chromatin architecture in the cell nucleus: on the way from structure to function
    Thomas Cremer
    Department Biologie II, Ludwig Maximilians University, Richard Wagner Street 10 I, 80333 Munich, Germany
    Biol Cell 96:555-67. 2004
    ..In addition we discuss the advantages and drawbacks of experimental approaches currently used to study nuclear architecture and function in fixed and living cells...
  9. ncbi request reprint Chromosome territories--a functional nuclear landscape
    Thomas Cremer
    Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, D 82152, Martinsried, Germany
    Curr Opin Cell Biol 18:307-16. 2006
    ....
  10. ncbi request reprint Rise, fall and resurrection of chromosome territories: a historical perspective. Part II. Fall and resurrection of chromosome territories during the 1950s to 1980s. Part III. Chromosome territories and the functional nuclear architecture: experiments and
    T Cremer
    Department of Biology II, 82152 Martinsried, Germany
    Eur J Histochem 50:223-72. 2006
    ....
  11. pmc Chromosome territories
    Thomas Cremer
    Biozentrum, Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, 82152 Martinsried, Germany
    Cold Spring Harb Perspect Biol 2:a003889. 2010
    ..The article concludes with a discussion of open questions and new experimental strategies to answer them...
  12. pmc Replication-timing-correlated spatial chromatin arrangements in cancer and in primate interphase nuclei
    Florian Grasser
    Department of Biology II, Human Genetics, Ludwig Maximilians University Munich, Planegg Martinsreid, Germany
    J Cell Sci 121:1876-86. 2008
    ....
  13. ncbi request reprint Spatial preservation of nuclear chromatin architecture during three-dimensional fluorescence in situ hybridization (3D-FISH)
    Irina Solovei
    Department of Biology II, Ludwig Maximilians University of Munich, Germany
    Exp Cell Res 276:10-23. 2002
    ..However, important ultrastructural details of the chromatin architecture are destroyed by the heat denaturation step, thus putting a limit to the usefulness of 3D-FISH analyses at nanometer scales...
  14. ncbi request reprint Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks
    Heiner Albiez
    Department of Biology II, LMU Biozentrum, Grosshaderner Strasse 2, 82152, Planegg Martinsried, Germany
    Chromosome Res 14:707-33. 2006
    ..These experiments, together with electron microscopic observations, demonstrate the existence of the IC as a dynamic, structurally distinct nuclear compartment, which is functionally linked with the chromatin compartment...
  15. pmc Common themes and cell type specific variations of higher order chromatin arrangements in the mouse
    Robert Mayer
    Department Biologie II, Ludwig Maximilians Universitat Munchen, Grosshaderner Str 2, 82152 Planegg Martinsried, Germany
    BMC Cell Biol 6:44. 2005
    ....
  16. ncbi request reprint Differences in centromere positioning of cycling and postmitotic human cell types
    Irina Solovei
    Department of Biology II, Humangenetik, Ludwig Maximillians University LMU, Richard Wagner Str 10, 80333, Munich, Germany
    Chromosoma 112:410-23. 2004
    ..This asymmetrical distribution of centromeres is considered to be a consequence of chromosome arrangement in anaphase rosettes...
  17. doi request reprint Multicolor 3D fluorescence in situ hybridization for imaging interphase chromosomes
    Marion Cremer
    Department of Biology II, Ludwig Maximilians University Biozentrum, Planegg Martinsried, Germany
    Methods Mol Biol 463:205-39. 2008
    ....
  18. ncbi request reprint Histone lysine methylation patterns in human cell types are arranged in distinct three-dimensional nuclear zones
    Roman Zinner
    Anthropology and Human Genetics, Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, D 82152, Martinsried, Germany
    Histochem Cell Biol 125:3-19. 2006
    ..Finally we demonstrated by simultaneous visualization of different histone lysine methylation sites that methylation patterns are organized in distinct nuclear zones with little apparent intermingling...
  19. doi request reprint Changes of higher order chromatin arrangements during major genome activation in bovine preimplantation embryos
    Daniela Koehler
    Division of Anthropology and Human Genetics, Biocenter, LMU Munich, Grosshadernerstr 2, D 82152 Martinsried, Germany
    Exp Cell Res 315:2053-63. 2009
    ..Since major genome activation in bovine embryos occurs at the 8- to 16-cell stage, our findings demonstrate a temporal correlation between transcriptional activation and a major rearrangement of chromatin topography in blastomere nuclei...
  20. pmc Radial chromatin positioning is shaped by local gene density, not by gene expression
    Katrin Küpper
    Department of Biology II, Anthropology and Human Genetics, Ludwig Maximilians University, Munich, Germany
    Chromosoma 116:285-306. 2007
    ..5 often loops out from the territory surface, gene-dense and highly expressed sequences were not generally found preferentially at the CT surface as previously suggested...
  21. doi request reprint Remodeling of nuclear architecture by the thiodioxoxpiperazine metabolite chaetocin
    Doris Illner
    Biozentrum, Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, 82152 Martinsried, Germany
    Exp Cell Res 316:1662-80. 2010
    ..The mechanisms involved in CICC formation remain to be fully explored...
  22. doi request reprint Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution
    Irina Solovei
    Division of Anthropology and Human Genetics, Biocenter, Ludwig Maximilians University Munich, Grosshadernerstrasse 2, 82152 Planegg Martinsried, Germany
    Cell 137:356-68. 2009
    ..Comparison of the two patterns suggests that the conventional architecture prevails in eukaryotic nuclei because it results in more flexible chromosome arrangements, facilitating positional regulation of nuclear functions...
  23. pmc The architecture of chicken chromosome territories changes during differentiation
    Sonja Stadler
    Department Biologie II, Biozentrum der Ludwig Maximilians Universität München, Planegg Martinsried, Germany
    BMC Cell Biol 5:44. 2004
    ..Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown...
  24. pmc Maintenance of imprinting and nuclear architecture in cycling cells
    Kathrin Teller
    Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, 82152 Planegg Martinsried, Germany
    Proc Natl Acad Sci U S A 104:14970-5. 2007
    ....
  25. doi request reprint Double-strand break-induced transcriptional silencing is associated with loss of tri-methylation at H3K4
    Doris M Seiler
    Department of Radiation Oncology, University Hospital of Munich, Schillerstr 42, 80336, Munich, Germany
    Chromosome Res 19:883-99. 2011
    ..Since no accumulation of the repressive mark H3K9me2 was found at damaged sites, we suggest that DSB-induced transcriptional silencing resembles polycomb-mediated silencing rather than heterochromatic silencing...
  26. ncbi request reprint Biochemistry meets nuclear architecture: multicolor immuno-FISH for co-localization analysis of chromosome segments and differentially expressed gene loci with various histone methylations
    Roman Zinner
    Anthropology and Human Genetics, Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, D 82152 Martinsried, Germany
    Adv Enzyme Regul 47:223-41. 2007
  27. ncbi request reprint Replication labeling patterns and chromosome territories typical of mammalian nuclei are conserved in the early metazoan Hydra
    Olga Alexandrova
    Department of Biology II, Cell and Developmental Biology, Ludwig Maximilians University, Munich, Germany
    Chromosoma 112:190-200. 2003
    ..Our results indicate the conservation of fundamental features of higher order chromatin arrangements throughout the evolution of metazoan animals and suggest the existence of conserved mechanism(s) controlling this architecture...
  28. doi request reprint The radial nuclear positioning of genes correlates with features of megabase-sized chromatin domains
    Alexandra C Kölbl
    Department Biologie II, Ludwig Maximilians Universitat Munchen, Planegg, Martinsried, Germany
    Chromosome Res 20:735-52. 2012
    ..Our results strongly support the hypothesis that knowledge of the local chromatin environment is essential to predict the radial nuclear position of a gene...
  29. pmc Inheritance of gene density-related higher order chromatin arrangements in normal and tumor cell nuclei
    Marion Cremer
    Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany
    J Cell Biol 162:809-20. 2003
    ..e., a CT #18 located more internally than a CT #19. This observation emphasizes a partial loss of radial chromatin order in tumor cell nuclei...
  30. pmc Evolutionary conservation of chromosome territory arrangements in cell nuclei from higher primates
    Hideyuki Tanabe
    Department of Biology II Human Genetics, University of Munich, Richard Wagner Strasse 10, 80333 München, Germany
    Proc Natl Acad Sci U S A 99:4424-9. 2002
    ..The evolutionarily conserved positioning of homologous chromosomes or chromosome segments in related species supports evidence for a functionally relevant higher-order chromatin arrangement that is correlated with gene-density...
  31. doi request reprint The potential of 3D-FISH and super-resolution structured illumination microscopy for studies of 3D nuclear architecture: 3D structured illumination microscopy of defined chromosomal structures visualized by 3D (immuno)-FISH opens new perspectives for stud
    Yolanda Markaki
    Biocenter, Department Biology II, Ludwig Maximilians University LMU, Martinsried, Germany
    Bioessays 34:412-26. 2012
    ....
  32. pmc Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes
    Andreas Bolzer
    Department of Biology II, Anthropology and Human Genetics, Ludwig Maximilians University, Munich, Germany
    PLoS Biol 3:e157. 2005
    ..Chromatin domains, which are gene-poor, form a layer beneath the nuclear envelope, while gene-dense chromatin is enriched in the nuclear interior. We discuss the possible functional implications of this finding...
  33. doi request reprint A top-down analysis of Xa- and Xi-territories reveals differences of higher order structure at ≥ 20 Mb genomic length scales
    Kathrin Teller
    Biocenter, Department Biology II, Ludwig Maximilians University LMU, Martinsried, Germany
    Nucleus 2:465-77. 2011
    ..In contrast to a previous report, genes subject to inactivation as well as escaping from inactivation were not excluded from the interior of the Barr body...
  34. ncbi request reprint Positioning of the mouse Hox gene clusters in the nuclei of developing embryos and differentiating embryoid bodies
    Christian Lanctôt
    Department Biology II, Ludwig Maximilians University Munich, Grosshadernerstr 2, Planegg Martinsried, 82152, Germany
    Exp Cell Res 313:1449-59. 2007
    ....
  35. ncbi request reprint Three-dimensional arrangements of centromeres and telomeres in nuclei of human and murine lymphocytes
    Claudia Weierich
    Department of Biology II, Human Genetics, Ludwig Maximillians University LMU, Richard Wagner Str 10, 80333 Munich, Germany
    Chromosome Res 11:485-502. 2003
    ..While the centromere of the active X often participated in the formation of centromere clusters, such a participation was never observed for the centromere of the inactive X...
  36. ncbi request reprint Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensions
    Christian Lanctôt
    Department Biologie II, Ludwig Maximilians Universitat, Grosshadernerstr 2, Planegg Martinsried, Germany
    Nat Rev Genet 8:104-15. 2007
    ..Understanding how the dynamic nature of the positioning of genetic material in the nuclear space and the higher-order architecture of the nucleus are integrated is therefore essential to our overall understanding of gene regulation...
  37. pmc Methyl CpG-binding proteins induce large-scale chromatin reorganization during terminal differentiation
    Alessandro Brero
    Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    J Cell Biol 169:733-43. 2005
    ..This MeCP2- and MBD2-mediated chromatin reorganization may thus represent a molecular link between nuclear genome topology and the epigenetic maintenance of cellular differentiation...
  38. pmc 4D chromatin dynamics in cycling cells: Theodor Boveri's hypotheses revisited
    Hilmar Strickfaden
    Department Biology II Anthropology and Human Genetics, LMU Biozentrum, Martinsried, Germany
    Nucleus 1:284-97. 2010
    ..A new model of chromatin dynamics is proposed. It suggests that long-range DNA-DNA interactions in cell nuclei may depend on a combination of rotational CT movements and locally constrained chromatin movements...
  39. doi request reprint Functional nuclear architecture studied by microscopy: present and future
    Jacques Rouquette
    Biocenter, Ludwig Maximilians University LMU, Martinsried, Germany
    Int Rev Cell Mol Biol 282:1-90. 2010
    ..This will make possible future analyses of cell type- and species-specific differences of nuclear architecture in more detail and to put different models to critical tests...
  40. doi request reprint Correlative microscopy of individual cells: sequential application of microscopic systems with increasing resolution to study the nuclear landscape
    Barbara Hübner
    Department Biology II, Anthropology and Human Genetics, Biocenter, Ludwig Maximilians University LMU, Martinsried, Germany
    Methods Mol Biol 1042:299-336. 2013
    ..We discuss problems involved in the comparison of images of a given cell nucleus recorded with different microscopic approaches, which requires not only a compensation for different resolutions but also for various distortions...
  41. doi request reprint Revealing the high-resolution three-dimensional network of chromatin and interchromatin space: a novel electron-microscopic approach to reconstructing nuclear architecture
    Jacques Rouquette
    Biocenter, Ludwig Maximilians University LMU, Grosshaderner Str 2, D 82152 Martinsried, Germany
    Chromosome Res 17:801-10. 2009
    ..Our results show the importance of the contribution of such reconstruction techniques to our understanding of the nuclear architecture...
  42. ncbi request reprint Light optical precision measurements of the active and inactive Prader-Willi syndrome imprinted regions in human cell nuclei
    Joachim Rauch
    Kirchhoff Institute of Physics, University of Heidelberg, Im Neuenheimer Feld 227, D 69120 Heidelberg, Germany
    Differentiation 76:66-82. 2008
    ..The possibilities to achieve this goal are discussed...
  43. ncbi request reprint Exploiting nuclear duality of ciliates to analyse topological requirements for DNA replication and transcription
    Jan Postberg
    Institute of Cell Biology, University of Witten Herdecke, Stockumer Str 10, 58453 Witten, Germany
    J Cell Sci 118:3973-83. 2005
    ..Moreover nuclear duality inherent to ciliates with their germline micronucleus and their somatic macronucleus may eventually provide further insight into epigenetic regulation of transcription, replication and nuclear differentiation...
  44. ncbi request reprint Epigenomic differentiation in mouse preimplantation nuclei of biparental, parthenote and cloned embryos
    Valeria Merico
    Dipartimento di Biologia Animale, Laboratorio di Biologia dello Sviluppo, Piazza Botta 9, Universita degli Studi di Pavia, 27100, Pavia, Italy
    Chromosome Res 15:341-60. 2007
    ..This finding suggests that the epigenome of the three types of embryos partially acts as a constraint of the nuclear organization of the three nuclear subcompartments analysed...
  45. ncbi request reprint Non-random radial arrangements of interphase chromosome territories: evolutionary considerations and functional implications
    Hideyuki Tanabe
    Cell Bank Laboratory, Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo, Japan
    Mutat Res 504:37-45. 2002
    ....
  46. ncbi request reprint Nuclear architecture: Is it important for genome function and can we prove it?
    Julio Mateos-Langerak
    Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
    J Cell Biochem 102:1067-75. 2007
    ..Numerous observations indicate a tight correlation between genome activity and nuclear and large-scale chromatin structure. However, causal relationships are rare. Here we explore how these might be uncovered...
  47. ncbi request reprint Non-rigid registration of 3D multi-channel microscopy images of cell nuclei
    Siwei Yang
    Biomedical Computer Vision Group, Dept Theoretical Bioinformatics, DKFZ Heidelberg, Germany
    Med Image Comput Comput Assist Interv 9:907-14. 2006
    ..We also describe an extension of our approach which is applied for the registration of 3D+t (4D) image series of moving cell nuclei...