Research Topics
Genomes and Genes | P CramerSummaryAffiliation: University of Munich Country: Germany Publications
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Detail Information
Publications
Functional architecture of RNA polymerase IClaus D Kuhn
Gene Center Munich and Center for Integrated Protein Science CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, 81377 Munich, Germany
Cell 131:1260-72. 2007..In contrast to Pol II, Pol I has a strong intrinsic 3'-RNA cleavage activity, which requires the C-terminal domain of subunit A12.2 and, apparently, enables ribosomal RNA proofreading and 3'-end trimming...
The spt5 C-terminal region recruits yeast 3' RNA cleavage factor IAndreas Mayer
Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich, Ludwig Maximilians Universitat Munchen, Munich, Germany
Mol Cell Biol 32:1321-31. 2012..Consistent with this model, the CTR interacts with CFI in vitro but is not required for pA site recognition and transcription termination in vivo...- The phosphatidylserine receptor from Hydra is a nuclear protein with potential Fe(II) dependent oxygenase activityMihai Cikala
Department Biologie II, Ludwig Maximilians University Munich, Luisenstr, 14 D 80333 Munich, Germany
BMC Cell Biol 5:26. 2004..Surface exposure of phosphatidylserine is recognised by a specific receptor (phosphatidylserine receptor, PSR) and is required for phagocytosis of apoptotic cells by macrophages and fibroblasts... - RNA polymerase II structure: from core to functional complexesPatrick Cramer
Institute of Biochemistry and Gene Center, University of Munich, Feodor Lynen Str 25, 81377 Munich, Germany
Curr Opin Genet Dev 14:218-26. 2004..The new studies also show that domains of transcription factors can enter polymerase openings, to modulate function during transcription... - Recent structural studies of RNA polymerases II and IIIP Cramer
Gene Center Munich, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Biochem Soc Trans 34:1058-61. 2006..Finally, we obtained the first structural information on Pol III, in the form of an 11-subunit model obtained by combining a homology model of the nine-subunit core enzyme with a new X-ray structure of the subcomplex C17/25... 
Structure of eukaryotic RNA polymerasesP Cramer
Gene Center Munich and Center for Integrated Protein Science CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, 81377 Munich, Germany
Annu Rev Biophys 37:337-52. 2008....- Towards molecular systems biology of gene transcription and regulationPatrick Cramer
Department of Biochemistry, Center for Integrated Protein Science CIPSM, Ludwig Maximilians University, Gene Center, Feodor Lynen Strasse 25, D 81377 Munich, Germany
Biol Chem 391:731-5. 2010..In this article, the efforts of our laboratory to move from an integrated structural biology of gene transcription towards molecular systems biology of gene regulation are reviewed... - Molecular biology. Self-correcting messagesPatrick Cramer
Gene Center Munich, Department of Chemistry and Biochemistry, , Feodor-Lynen-Strasse 25, 81377 Munich, Germany
Science 313:447-8. 2006 - Common structural features of nucleic acid polymerasesP Cramer
Institute of Biochemistry, Gene Center, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
Bioessays 24:724-9. 2002..All polymerases may undergo "induced fit" upon nucleic acid binding, but the underlying conformational changes differ... - Multisubunit RNA polymerasesPatrick Cramer
Institute of Biochemistry, Gene Center, University of Munich, Feodor Lynen Strasse 25, 81377, Munich, Germany
Curr Opin Struct Biol 12:89-97. 2002..Together with a further structure of eukaryotic RNAP bound to DNA and RNA, these results elucidate many aspects of the transcription mechanism, including initiation, elongation, nucleotide addition, processivity and proofreading... - Deciphering the RNA polymerase II structure: a personal perspectivePatrick Cramer
Gene Center Munich, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, D 81377 Munich, Germany
Nat Struct Mol Biol 13:1042-4. 2006..This year's Nobel laureate in chemistry is Roger Kornberg. Patrick Cramer gives a personal account of how the Kornberg laboratory determined the structure of the RNA polymerase II core enzyme... - Structure of an archaeal RNA polymeraseAnselm G Kusser
Gene Center Munich and Center for integrated Protein Science CiPS M, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, 81377 Munich, Germany
J Mol Biol 376:303-7. 2008..The structural organization of the archaeal RNA polymerase serves as a reference for future structure-function analysis of the transcription mechanism and allows for evolutionary comparisons... - Structure and in vivo requirement of the yeast Spt6 SH2 domainStefan Dengl
Gene Center and Center for Integrated Protein Science Munich, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Munich, Germany
J Mol Biol 389:211-25. 2009..Differential gene expression analysis reveals that the SH2 domain is required for normal expression of a subset of yeast genes, and is consistent with multiple functions of Spt6 in chromatin transcription... - Structure-function studies of the RNA polymerase II elongation complexFlorian Brueckner
Department of Chemistry and Biochemistry, Gene Center Munich and Center for Integrated Protein Science CIPSM, Ludwig Maximilians Universitat Munchen, Munich, Germany
Acta Crystallogr D Biol Crystallogr 65:112-20. 2009.... - Structural basis of transcription inhibition by alpha-amanitin and implications for RNA polymerase II translocationFlorian Brueckner
Gene Center and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Nat Struct Mol Biol 15:811-8. 2008.... - Molecular basis of RNA-dependent RNA polymerase II activityElisabeth Lehmann
Gene Center Munich and Center for Integrated Protein Science CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Nature 450:445-9. 2007..The RdRP activity of Pol II provides a missing link in molecular evolution, because it suggests that Pol II evolved from an ancient replicase that duplicated RNA genomes... - Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factorsAnton Meinhart
Department of Chemistry and Biochemistry, Gene Center, University of Munich, 81377 Munich, Germany
Nature 430:223-6. 2004..The model suggests that, during the mRNA transcription-processing cycle, compact spiral regions in the CTD are unravelled and regenerated in a phosphorylation-dependent manner... - RNA polymerase I contains a TFIIF-related DNA-binding subcomplexSebastian R Geiger
Gene Center and Department of Biochemistry, Center for Integrated Protein Science CIPSM, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Mol Cell 39:583-94. 2010..Thus, Pol I/III subunits that have no counterparts in Pol II are evolutionarily related to Pol II initiation factors and may have evolved to mediate promoter specificity and transcription processivity... - Structure and function of RNA polymerase IIPatrick Cramer
Institute of Biochemistry and Gene Center, University of Munich, 81377 Munich, Germany
Adv Protein Chem 67:1-42. 2004 - CPD damage recognition by transcribing RNA polymerase IIFlorian Brueckner
Munich Center for Integrated Protein Science CiPS, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Science 315:859-62. 2007..This is consistent with nonallosteric recruitment of repair factors and excision of a lesion-containing DNA fragment in the presence of Pol II... - Fluorescence detection of nucleic acids and proteins in multi-component crystalsHubert Kettenberger
Gene Center, University of Munich, Department of Chemistry and Biochemistry, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
Acta Crystallogr D Biol Crystallogr 62:146-50. 2006..Additional experiments suggest the general applicability of these fluorescence assays for the characterization of crystal contents... - Structure and carboxyl-terminal domain (CTD) binding of the Set2 SRI domain that couples histone H3 Lys36 methylation to transcriptionErika Vojnic
Gene Center, Department of Chemistry and Biochemistry, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
J Biol Chem 281:13-5. 2006..Unexpectedly, the SRI domain fold resembles the structure of an RNA polymerase-interacting domain in bacterial sigma factors (domain sigma2 in sigma70)... - Architecture of initiation-competent 12-subunit RNA polymerase IIKarim Jean Armache
Institute of Biochemistry and Gene Center, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
Proc Natl Acad Sci U S A 100:6964-8. 2003..The core-Rpb7 interaction and some functions of Rpb4/7 are apparently conserved in all eukaryotic and archaeal RNA polymerases but not in the bacterial enzyme... - An extended winged helix domain in general transcription factor E/IIE alphaAnton Meinhart
Institute of Biochemistry, Gene Center, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
J Biol Chem 278:48267-74. 2003..Homology modeling shows that the TFE domain is conserved in TFIIE alpha, including the potential functional surfaces... - Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20Laurent Lariviere
Gene Center Munich, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, 81377 Munich, Germany
Nat Struct Mol Biol 13:895-901. 2006.... - Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIISHubert Kettenberger
Gene Center, Department of Chemistry and Biochemistry, Ludwig Maximilians University of Munich, Feodor Lynen Str 25, 81377 Munich, Germany
Mol Cell 16:955-65. 2004..Binding of the elongation factor TFIIS realigns RNA in the active center, possibly converting the elongation complex to an alternative state less prone to stalling... - Mechanism of transcriptional stalling at cisplatin-damaged DNAGerke E Damsma
Center for Integrated Protein Science CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Nat Struct Mol Biol 14:1127-33. 2007..The stalling mechanism differs from that of Pol II stalling at a photolesion, which involves delivery of the lesion to the active site and lesion-templated misincorporation that blocks transcription... - Identification, structure, and functional requirement of the Mediator submodule Med7N/31Tobias Koschubs
Gene Center and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universität LMU München, Munich, Germany
EMBO J 28:69-80. 2009..Comparative phenotyping and transcriptome profiling identify specific and overlapping functions of different Mediator submodules... - RNA polymerase fidelity and transcriptional proofreadingJasmin F Sydow
Gene Center Munich and Center for Integrated Protein Science Munich, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, 81377 Munich, Germany
Curr Opin Struct Biol 19:732-9. 2009..Since cleavage occurs at the same active site that is used for polymerization, the RNAP proofreading mechanism differs from that used by DNAPs, which contain a distinct nuclease specific active site... - Molecular basis of transcriptional mutagenesis at 8-oxoguanineGerke E Damsma
Gene Center and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
J Biol Chem 284:31658-63. 2009..The misincorporated adenine escapes intrinsic proofreading, resulting in transcriptional mutagenesis that is observed directly by mass spectrometric RNA analysis... - Nano positioning system reveals the course of upstream and nontemplate DNA within the RNA polymerase II elongation complexJoanna Andrecka
Department of Chemistry and Biochemistry and Center for Integrated Protein Science München, Ludwig Maximilians Universitat Munchen, Butenandtstr 11, 81377 Munchen, Germany
Nucleic Acids Res 37:5803-9. 2009..Comparison with published data suggests that the architecture of the complete EC is conserved from bacteria to eukaryotes and that upstream DNA is relocated during the initiation-elongation transition... - Crystallization of RNA polymerase I subcomplex A14/A43 by iterative prediction, probing and removal of flexible regionsSebastian R Geiger
Gene Center Munich, Center for Integrated Protein Science CIPSM and Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:413-8. 2008..This strategy should enable the crystallization of other proteins and subcomplexes with multiple flexibilities, as required for hybrid structure solution of large macromolecular assemblies... - Structure-system correlation identifies a gene regulatory Mediator submoduleLaurent Lariviere
Gene Center Munich and Center for Integrated Protein Science CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, 81377 Munich, Germany
Genes Dev 22:872-7. 2008..The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation... - Uniform transitions of the general RNA polymerase II transcription complexAndreas Mayer
Gene Center Munich and Department of Biochemistry, Center for Integrated Protein Science, Ludwig Maximilians Universitat Munchen, Munich, Germany
Nat Struct Mol Biol 17:1272-8. 2010..Transitions are uniform and independent of gene length, type and expression... - Structural basis of transcription: mismatch-specific fidelity mechanisms and paused RNA polymerase II with frayed RNAJasmin F Sydow
Department of Chemistry and Biochemistry, Gene Center Munich and Center for Integrated Protein Science CIPSM, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Mol Cell 34:710-21. 2009.... - Structure and mechanism of RNA polymerase II CTD phosphatasesTomislav Kamenski
Department of Chemistry and Biochemistry, Gene Center, University of Munich, Feodor Lynen Str 25, 81377 Munich, Germany
Mol Cell 15:399-407. 2004..Fcp1 specificity may additionally arise from phosphatase recruitment near the CTD via the Pol II subcomplex Rpb4/7, which is shown to be required for binding of Fcp1 to the polymerase in vitro... - Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAsHubert Kettenberger
Gene Center, University of Munich, Department of Chemistry and Biochemistry, Feodor-Lynen-Str. 25, 81377 Munich, Germany
Nat Struct Mol Biol 13:44-8. 2006..The structure also indicates that A-form RNA could interact with Pol II similarly to a B-form DNA promoter, as suggested for the bacterial transcription inhibitor 6S RNA... - Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavageHubert Kettenberger
Institute of Biochemistry, Gene Center, University of Munich, Feodor Lynen Str 25, 81377 Munich, Germany
Cell 114:347-57. 2003..Our results support the idea that Pol II contains a single tunable active site for RNA polymerization and cleavage, in contrast to DNA polymerases with two separate active sites for DNA polymerization and cleavage... - The dynamic machinery of mRNA elongationKarim-Jean Armache
Department of Chemistry and Biochemistry, Gene Center, Ludwig-Maximilians-University of Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
Curr Opin Struct Biol 15:197-203. 2005.... - A structural perspective of CTD functionAnton Meinhart
Department of Chemistry and Biochemistry, Gene Center, University of Munich (LMU, 81377 Munich, Germany
Genes Dev 19:1401-15. 2005..The catalytic mechanisms of CTD kinases and phosphatases are known, but the basis for CTD specificity of these enzymes remains to be understood... - DNA photodamage recognition by RNA polymerase IIFlorian Brueckner
Gene Center Munich, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, D 81377 Munich, Germany
FEBS Lett 581:2757-60. 2007..The study provided the molecular basis for recognition of a damaged DNA by Pol II, which is the first step in transcription-coupled DNA repair (TCR). The results have general implications for damage recognition and the TCR mechanism... - A nano-positioning system for macromolecular structural analysisAdam Muschielok
Department of Chemistry and Biochemistry and Center for Integrated Protein Science München, Ludwig Maximilians Universitat Munchen, Butenandtstrasse 11, 81377 Munchen, Germany
Nat Methods 5:965-71. 2008.... - Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme modelAnna J Jasiak
Department of Chemistry and Biochemistry, Gene Center, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
Mol Cell 23:71-81. 2006..During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro... - Torpedo nuclease Rat1 is insufficient to terminate RNA polymerase II in vitroStefan Dengl
Gene Center and Center for Integrated Protein Science Munich, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
J Biol Chem 284:21270-9. 2009..Thus, pol II termination apparently requires a factor or several factors in addition to Rat1, Rai1, and Rtt103, post-translational modifications of these factors, or unusual reaction conditions... - Structure of a bifunctional DNA primase-polymeraseGeorg Lipps
Institute of Biochemistry, University of Bayreuth, Universitatsstrasse 30, D 95447 Bayreuth, Germany
Nat Struct Mol Biol 11:157-62. 2004..We propose that archaeal and eukaryotic primases and the prim-pol domain have a common evolutionary ancestor, a bifunctional replicase for small DNA genomes... - Single-molecule tracking of mRNA exiting from RNA polymerase IIJoanna Andrecka
Munich Center for Integrated Protein Science CiPS and Center for Nanoscience CeNS, Ludwig Maximilians Universitat Munchen, 81377 Munich, Germany
Proc Natl Acad Sci U S A 105:135-40. 2008..RNA further extends toward the linker connecting to the polymerase C-terminal repeat domain (CTD), which binds the 5'-capping enzyme and other RNA processing factors... - Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplexAnna J Jasiak
Gene Center and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 25, D 81377 Munich, Germany
J Biol Chem 283:26423-7. 2008..Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle... - A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Srb7) heterodimerSonja Baumli
Gene Center, University of Munich Ludwig Maximilians Universität, Department of Chemistry and Biochemistry, Feodor Lynen Strasse 25, 81377 Munich, Germany
J Biol Chem 280:18171-8. 2005..MED21 hinge could account for changes in overall Mediator structure upon binding to Pol II or activators. Our results support the idea that transcription regulation involves conformational changes within the general machinery... - Structures of complete RNA polymerase II and its subcomplex, Rpb4/7Karim Jean Armache
Gene Center, University of Munich LMU, Department of Chemistry and Biochemistry, Feodor Lynen Strasse 25, 81377 Munich, Germany
J Biol Chem 280:7131-4. 2005.... - Molecular basis of RNA polymerase III transcription repression by Maf1Alessandro Vannini
Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich CIPSM, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Cell 143:59-70. 2010..These results explain how Maf1 specifically represses transcription initiation from Pol III promoters and indicate that Maf1 also prevents reinitiation by binding Pol III during transcription elongation... - RNA polymerase II-TFIIB structure and mechanism of transcription initiationDirk Kostrewa
Gene Center Munich and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Nature 462:323-30. 2009..Synthesis of the RNA chain and rewinding of upstream DNA displace the B-reader and B-linker, respectively, to trigger B release and elongation complex formation... - A movie of the RNA polymerase nucleotide addition cycleFlorian Brueckner
Gene Center and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
Curr Opin Struct Biol 19:294-9. 2009..The movie facilitates teaching and a mechanistic analysis of transcription and can be downloaded from http://www.lmb.uni-muenchen.de/cramer/pr-materials... - Structure of the mediator subunit cyclin C and its implications for CDK8 functionSabine Hoeppner
Gene Center, University of Munich LMU, Department of Chemistry and Biochemistry, Feodor Lynen Str 25, 81377 Munich, Germany
J Mol Biol 350:833-42. 2005..In CDK8, this loop is longer and the threonine is absent, suggesting an alternative mechanism of activation that we discuss based on a CDK8-cyclin C model... - The mRNA transcription/processing factor Ssu72 is a potential tyrosine phosphataseAnton Meinhart
Institute of Biochemistry, Gene Center, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
J Biol Chem 278:15917-21. 2003..The novel Ssu72 activity may regulate coupling events during mRNA biogenesis... - The archaeo-eukaryotic primase of plasmid pRN1 requires a helix bundle domain for faithful primer synthesisKirsten Beck
Institute of Biochemistry, University of Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Germany
Nucleic Acids Res 38:6707-18. 2010..Furthermore, we identified mutants of the C-terminal domain, which are defective in template binding, dinucleotide formation and conformation change prior to DNA extension... - Preparation and topology of the Mediator middle moduleTobias Koschubs
Department of Biochemistry, Gene Center Munich and Center for Integrated Protein Science Munich, Ludwig Maximilians Universitat Munchen, Feodor Lynen St 25, 81377 Munich, Germany
Nucleic Acids Res 38:3186-95. 2010..The subunits, Med1 and Med10, which bridge to the Mediator tail module, bind to both Med7 and Med4... - Gene transcription: extending the messagePatrick Cramer
Nature 448:142-3. 2007 - Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active siteEkaterina Kashkina
Department of Cell Biology, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Stratford, NJ 08084, USA
J Biol Chem 282:21578-82. 2007..These data argue against a model in which multiple NTPs are lined up downstream of the active site... - Finding the right spot to start transcriptionPatrick Cramer
Nat Struct Mol Biol 14:686-7. 2007 - Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolutionDavid A Bushnell
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305-5126, USA
Proc Natl Acad Sci U S A 99:1218-22. 2002..The structure of the complex indicates the likely basis of inhibition and gives unexpected insight into the transcription mechanism... - Structure-function analysis of the RNA polymerase cleft loops elucidates initial transcription, DNA unwinding and RNA displacementSouad Naji
Lehrstuhl fur Mikrobiologie und Archaeenzentrum, Universitat Regensburg, Universitatsstrasse 31, D 93053 Regensburg, Germany
Nucleic Acids Res 36:676-87. 2008..During elongation, downstream DNA strand separation required template strand binding to an invariant arginine in switch 2, and apparently interaction of an invariant arginine in fork 2 with the non-template strand... - Template misalignment in multisubunit RNA polymerases and transcription fidelityEkaterina Kashkina
Department of Cell Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, 42 East Laurel Road, Stratford, New Jersey 08084, USA
Mol Cell 24:257-66. 2006..Analysis of the effects of different template topologies on misincorporation indicates that the duplex DNA immediately downstream of the active site plays an important role in transcription fidelity... - Structural biology of RNA polymerase III: mass spectrometry elucidates subcomplex architectureKristina Lorenzen
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Chemistry, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
Structure 15:1237-45. 2007..These data suggest that Pol III consists of a 10 subunit Pol II-like core; the peripheral heterodimers C17/25, C53/37, and C82/34; and subunit C31, which bridges between C82/34, C17/25, and the core... - Archaeal transcription: function of an alternative transcription factor B from Pyrococcus furiosusMichael Micorescu
Department of Biology, Portland State University, P O Box 751, Portland, OR 97207, USA
J Bacteriol 190:157-67. 2008..The results indicate that the TFB N-terminal region, containing conserved Zn ribbon and B-finger motifs, is important in promoter opening and that TFE can compensate for defects in the N terminus through enhancement of promoter opening... - Multiplexed proteomics mapping of yeast RNA polymerase II and III allows near-complete sequence coverage and reveals several novel phosphorylation sitesShabaz Mohammed
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Chemistry, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
Anal Chem 80:3584-92. 2008..The described multiplexed proteomics approach is generic and reveals that it is possible to map a protein complex to near completion while applying less than 5 mug (approximately 10 pmol) of total starting material...