Research Topics
Genomes and Genes | Ralf J BraunSummaryAffiliation: University of Bayreuth Location: , Germany Publications
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Detail Information
Publications
Improved proteome analysis of Saccharomyces cerevisiae mitochondria by free-flow electrophoresisHans Zischka
GSF Research Centre for Environment and Health, Institute of Human Genetics, Oberschleissheim, Germany
Proteomics 3:906-16. 2003..Therefore these results represent a feasible approach for an in-depth proteome analysis of mitochondria and possibly other organelles...
Mitochondrial dynamics in yeast cell death and agingRalf J Braun
Institut fur Zellbiologie, Universitat Bayreuth, 95440 Bayreuth, Germany
Biochem Soc Trans 39:1520-6. 2011..We highlight the roles of the components of the mitochondrial fusion and fission machinery that affect and regulate cell death and aging...
A new Canterbury tale: the eighth International Meeting on Yeast Apoptosis in Canterbury, UK, 2-6 May 2011D Carmona-Gutierrez
1 Institute of Molecular Biosciences, University of Graz, Graz, Austria 2 Institute of Biochemistry, Technical University of Graz, Graz, Austria
Cell Death Differ 18:1948-9. 2011- Neurotoxic 43-kDa TAR DNA-binding protein (TDP-43) triggers mitochondrion-dependent programmed cell death in yeastRalf J Braun
Institute of Molecular Biosciences, University of Graz, Graz, Austria
J Biol Chem 286:19958-72. 2011..These data demonstrate that mitochondria and oxidative stress are important to TDP-43-triggered cell death in yeast and may suggest a similar role in human TDP-43 pathologies... - Nervous yeast: modeling neurotoxic cell deathRalf J Braun
Institute of Molecular Biosciences, Department of Microbiology, Karl Franzens University of Graz, Graz, Austria
Trends Biochem Sci 35:135-44. 2010.... - 16-BAC/SDS-PAGE analysis of membrane proteins of yeast mitochondria purified by free flow electrophoresisRalf J Braun
GSF National Research Centre for Environment and Health, Institute of Human Genetics, Munich, Germany
Methods Mol Biol 528:83-107. 2009..Proteins were identified by Maldi-Tof mass spectrometry and peptide mass fingerprinting... - Purification of Saccharomyces cerevisiae mitochondria by zone electrophoresis in a free flow deviceHans Zischka
GSF National Research Center for Environment and Health, Institute of Toxicology, Munich Neuherberg, Germany
Methods Mol Biol 432:51-64. 2008..The purified mitochondria can be collected by centrifugation and used for further experiments and analysis such as sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting, 2-DE or electron microscopy... - Mechanisms of Cdc48/VCP-mediated cell death: from yeast apoptosis to human diseaseRalf J Braun
Institute of Human Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Munich Neuherberg, Germany
Biochim Biophys Acta 1783:1418-35. 2008..This complex role in cell death pathways could correspond with the various pathophysiological conditions Cdc48/VCP is involved in... - Two-dimensional electrophoresis of membrane proteinsRalf J Braun
GSF National Research Center for Environment and Health, Institute of Human Genetics, Ingolstaedter Landstrasse 1, 85764, Munich Neuherberg, Germany
Anal Bioanal Chem 389:1033-45. 2007..Finally, the separation of native membrane protein complexes through the application of blue and clear native gel electrophoresis (BN/CN-PAGE) is reviewed, as well as the free-flow electrophoresis (FFE) of membranes... - Differential analysis of Saccharomyces cerevisiae mitochondria by free flow electrophoresisHans Zischka
Institutes of Human Genetics and Toxicology, GSF National Research Center for Environment and Health, 85764 Munich Neuherberg, Germany
Mol Cell Proteomics 5:2185-200. 2006..This result demonstrates that ZE-FFE may give important information on the specific properties of subpopulations of a mitochondrial preparation allowing a further detailed functional analysis... - Crucial mitochondrial impairment upon CDC48 mutation in apoptotic yeastRalf J Braun
GSF National Research Center for Environment and Health, Institute of Human Genetics, Ingolstaedter Landstrasse 1, Munich Neuherberg D 85764, Germany
J Biol Chem 281:25757-67. 2006..Concomitantly, emergence of caspase-like enzymatic activity occurs suggesting a role for caspases in the cell death process. These data strongly point for the first time to a mitochondrial involvement in Cdc48p/VCP-dependent apoptosis... - MMI1 (YKL056c, TMA19), the yeast orthologue of the translationally controlled tumor protein (TCTP) has apoptotic functions and interacts with both microtubules and mitochondriaMark Rinnerthaler
Department of Cell Biology, Division of Genetics, University of Salzburg, Austria
Biochim Biophys Acta 1757:631-8. 2006..Our results so far indicate that Mmi1p is one of the few proteins establishing a functional link between microtubules and mitochondria which may be needed for correct localization of mitochondria during cell division... - The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activityChristian Johannes Gloeckner
GSF National Research Center for Environment and Health, Institute of Human Genetics, Munich Neuherberg, Germany
Hum Mol Genet 15:223-32. 2006..This suggests that the pathology of PD caused by the I2020T mutation is associated with an increase rather than a loss in LRRK2 kinase activity... - Ceramide triggers metacaspase-independent mitochondrial cell death in yeastDidac Carmona-Gutierrez
Institute of Molecular Biosciences, University of Graz, Graz, Austria
Cell Cycle 10:3973-8. 2011..Altogether, our results point to a pivotal role of mitochondria in ceramide-induced yeast cell death...