W Boos

Summary

Affiliation: University of Konstanz
Country: Germany

Publications

  1. ncbi request reprint Purification and properties of a periplasmic protein related to sn-glycerol-3-phosphate transport in Escherichia coli
    W Boos
    Eur J Biochem 72:571-81. 1977
  2. pmc Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli
    R Hengge-Aronis
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 173:7918-24. 1991
  3. pmc Characteristics of a ugp-encoded and phoB-dependent glycerophosphoryl diester phosphodiesterase which is physically dependent on the ugp transport system of Escherichia coli
    P Brzoska
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Bacteriol 170:4125-35. 1988
  4. ncbi request reprint Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors
    A Scholle
    Mol Gen Genet 208:247-53. 1987
  5. ncbi request reprint Mapping of mglB, the structural gene of the galactose-binding protein of Escherichia coli
    W Boos
    Mol Gen Genet 184:508-18. 1981
  6. pmc Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation
    W Boos
    Department of Biology, University of Konstanz, Germany
    Microbiol Mol Biol Rev 62:204-29. 1998
  7. ncbi request reprint Learning new tricks from an old dog: MalT of the Escherichia coli maltose system is part of a complex regulatory network
    W Boos
    Department of Biology, University of Konstanz, 78457 Konstanz, Germany
    Trends Genet 16:404-9. 2000
  8. pmc Molecular analysis of treB encoding the Escherichia coli enzyme II specific for trehalose
    W Klein
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 177:4043-52. 1995
  9. ncbi request reprint The role of the trehalose system in regulating the maltose regulon of Escherichia coli
    K Decker
    Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    Mol Microbiol 32:777-88. 1999
  10. ncbi request reprint Negative transcriptional regulation of a positive regulator: the expression of malT, encoding the transcriptional activator of the maltose regulon of Escherichia coli, is negatively controlled by Mlc
    K Decker
    Department of Biology, University of Konstanz, Germany
    Mol Microbiol 27:381-90. 1998

Collaborators

Detail Information

Publications73

  1. ncbi request reprint Purification and properties of a periplasmic protein related to sn-glycerol-3-phosphate transport in Escherichia coli
    W Boos
    Eur J Biochem 72:571-81. 1977
    ..coli...
  2. pmc Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli
    R Hengge-Aronis
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 173:7918-24. 1991
    ..Neither sigma S nor trehalose, however, is required for the development of adaptive thermotolerance in growing cells, which might be controlled by sigma E...
  3. pmc Characteristics of a ugp-encoded and phoB-dependent glycerophosphoryl diester phosphodiesterase which is physically dependent on the ugp transport system of Escherichia coli
    P Brzoska
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Bacteriol 170:4125-35. 1988
    ..The product of the ugpQ gene, expressed in minicells, has an apparent molecular weight of 17,500. We present evidence that only one major phoB-dependent promoter controls all ugp genes...
  4. ncbi request reprint Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors
    A Scholle
    Mol Gen Genet 208:247-53. 1987
    ..The amino acid sequence deduced for the beginning of the MglA protein showed homology with a family of polypeptides that contain an ATP-binding site and are components of binding-protein-dependent transport systems...
  5. ncbi request reprint Mapping of mglB, the structural gene of the galactose-binding protein of Escherichia coli
    W Boos
    Mol Gen Genet 184:508-18. 1981
    ..These strains appeared unstable. Upon reisolation from purified stock cultures kept in glycerol at -20 degrees C, colonies could be isolated that carried only mutant or wild-type protein...
  6. pmc Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation
    W Boos
    Department of Biology, University of Konstanz, Germany
    Microbiol Mol Biol Rev 62:204-29. 1998
    ....
  7. ncbi request reprint Learning new tricks from an old dog: MalT of the Escherichia coli maltose system is part of a complex regulatory network
    W Boos
    Department of Biology, University of Konstanz, 78457 Konstanz, Germany
    Trends Genet 16:404-9. 2000
    ..MalT, the central activator of the mal genes, is the key element in this complex regulatory network and integrates the different signals to give an appropriate transcriptional response...
  8. pmc Molecular analysis of treB encoding the Escherichia coli enzyme II specific for trehalose
    W Klein
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 177:4043-52. 1995
    ..Instead, enzyme IITre-mediated phosphorylation of trehalose requires the activity of enzyme IIAGlc, a component of the major glucose transport system...
  9. ncbi request reprint The role of the trehalose system in regulating the maltose regulon of Escherichia coli
    K Decker
    Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    Mol Microbiol 32:777-88. 1999
    ..Extracts containing TreC transformed [14C]-maltose into another 14C-labelled compound (preliminarily identified as maltose 1-phosphate) that is likely to be an alternative inducer of the maltose system...
  10. ncbi request reprint Negative transcriptional regulation of a positive regulator: the expression of malT, encoding the transcriptional activator of the maltose regulon of Escherichia coli, is negatively controlled by Mlc
    K Decker
    Department of Biology, University of Konstanz, Germany
    Mol Microbiol 27:381-90. 1998
    ..As Mlc also regulates another operon (manXYZ, see pages 369-379 of this issue), it may very well constitute a new global regulator of carbohydrate utilization...
  11. pmc Molecular characterization of glucokinase from Escherichia coli K-12
    D Meyer
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 179:1298-306. 1997
    ..This demonstrates that free internal glucose plays an essential role in the formation of the endogenous inducer of the maltose system...
  12. ncbi request reprint Binding protein-dependent ABC transport system for glycerol 3-phosphate of Escherichia coli
    W Boos
    Faculty of Biology, University of Konstanz, Germany
    Methods Enzymol 292:40-51. 1998
  13. pmc MalI, a novel protein involved in regulation of the maltose system of Escherichia coli, is highly homologous to the repressor proteins GalR, CytR, and LacI
    J Reidl
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Bacteriol 171:4888-99. 1989
    ..Upstream to malI we observed a divergent open reading frame that extended to the end of the sequenced DNA...
  14. ncbi request reprint The MalT-dependent and malZ-encoded maltodextrin glucosidase of Escherichia coli can be converted into a dextrinyltransferase by a single mutation
    R Peist
    Department of Biology, University of Konstanz, Germany
    J Biol Chem 271:10681-9. 1996
    ..It was the MalZ292-associated transferase reaction that allowed the utilization of maltose. In addition, we discovered that mutant and wild type enzyme alike were highly active as gamma-cyclodextrinases...
  15. ncbi request reprint The mglB sequence of Salmonella typhimurium LT2; promoter analysis by gene fusions and evidence for a divergently oriented gene coding for the mgl repressor
    D Benner-Luger
    Department of Biology, University of Konstanz, Federal Republic of Germany
    Mol Gen Genet 214:579-87. 1988
    ..We cloned the gene located downstream to PD and found it to strongly repress the expression of the mgl operon. We termed this gene mglD. The presence of D-fucose abolished the repression caused by the plasmid-encoded mglD gene product...
  16. pmc Signal transduction between a membrane-bound transporter, PtsG, and a soluble transcription factor, Mlc, of Escherichia coli
    S J Lee
    Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    EMBO J 19:5353-61. 2000
    ..The control of Mlc activity by sequestration to a transport protein represents a novel form of signal transduction in gene regulation...
  17. pmc Trehalose-6-phosphate hydrolase of Escherichia coli
    M Rimmele
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 176:5654-64. 1994
    ..This report corrects our previous view on the function of the treC gene product as an amylotrehalase, which was based on the analysis of the metabolic products of trehalose metabolism in whole cells...
  18. ncbi request reprint Glycerol-3-phosphate-mediated repression of malT in Escherichia coli does not require metabolism, depends on enzyme IIAGlc and is mediated by cAMP levels
    T Eppler
    Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    Mol Microbiol 33:1221-31. 1999
    ..Other sugars that are not transported by the phosphotransferase system, most notably D-xylose, showed the same effect as glycerol...
  19. pmc Characterization of a cytoplasmic trehalase of Escherichia coli
    R Horlacher
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 178:6250-7. 1996
    ..Mutants producing 17-fold more TreF than does the wild type were isolated...
  20. ncbi request reprint The crystal structure of a liganded trehalose/maltose-binding protein from the hyperthermophilic Archaeon Thermococcus litoralis at 1.85 A
    J Diez
    Department of Biology, University of Konstanz, 78457 Konstanz, Germany
    J Mol Biol 305:905-15. 2001
    ..Specifically, we find helices that are longer than their structurally equivalent counterparts, and fewer internal cavities...
  21. ncbi request reprint Characterization of TreR, the major regulator of the Escherichia coli trehalose system
    R Horlacher
    Department of Biology, University of Konstanz, 78434 Konstanz, Germany
    J Biol Chem 272:13026-32. 1997
    ..Nuclease protection experiments revealed a palindromic sequence located directly upstream of the -35 promoter sequence of treB that functions as the operator of the system...
  22. ncbi request reprint The repression of trehalose transport and metabolism in Escherichia coli by high osmolarity is mediated by trehalose-6-phosphate phosphatase
    W Klein
    Department of Biology, University of Konstanz, Germany
    Res Microbiol 142:359-71. 1991
    ..There is another gene locus near 84 min on the chromosome, that we termed otsR, which is involved in the regulation of otsA/B and possibly otsP. The nature of this regulatory gene is unclear at present...
  23. pmc The activities of the Escherichia coli MalK protein in maltose transport, regulation, and inducer exclusion can be separated by mutations
    S Kühnau
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Bacteriol 173:2180-6. 1991
    ....
  24. pmc Crystal structure of the effector-binding domain of the trehalose-repressor of Escherichia coli, a member of the LacI family, in its complexes with inducer trehalose-6-phosphate and noninducer trehalose
    U Hars
    Department of Biology, University of Konstanz, Germany
    Protein Sci 7:2511-21. 1998
    ..The ratio of the concentrations of trehalose-6-phosphate and trehalose thus is used to switch between the two alternative metabolic uses of trehalose as an osmoprotectant and as a carbon source...
  25. pmc Glycerol facilitator of Escherichia coli: cloning of glpF and identification of the glpF product
    G Sweet
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Bacteriol 172:424-30. 1990
    ..The GlpF protein appeared in the membrane fraction of plasmid-bearing strains and had an apparent Mr of 25,000...
  26. ncbi request reprint The malZ gene of Escherichia coli, a member of the maltose regulon, encodes a maltodextrin glucosidase
    S Tapio
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Biol Chem 266:19450-8. 1991
    ..Homology to glucosidases, alpha-amylases, and pullulanases were observed. Conserved regions thought to represent active sites in dextrin hydrolases were found in the MalZ protein...
  27. pmc Glycerol kinase of Escherichia coli is activated by interaction with the glycerol facilitator
    R T Voegele
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 175:1087-94. 1993
    ..We conclude that there is an interaction between the glycerol facilitator protein and glycerol kinase that stimulates kinase activity, analogous to the hexokinase- and glycerol kinase-porin interactions in mitochondria...
  28. ncbi request reprint Trehalase of Escherichia coli. Mapping and cloning of its structural gene and identification of the enzyme as a periplasmic protein induced under high osmolarity growth conditions
    W Boos
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Biol Chem 262:13212-8. 1987
    ..Thus, the plasmid carries the structural gene for the periplasmic trehalase and not just a gene involved in the regulation of the enzyme...
  29. pmc The pho regulon-dependent Ugp uptake system for glycerol-3-phosphate in Escherichia coli is trans inhibited by Pi
    P Brzoska
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 176:15-20. 1994
    ..5 mM. Cells had to be starved for Pi in order to observe this inhibition. We conclude that the activity of the Ugp transport system is controlled by the level of internal phosphate...
  30. pmc Improved method for the preparative synthesis of labeled trehalose of high specific activity by Escherichia coli
    R Horlacher
    Department of Biology, University of Konstanz, Germany
    Appl Environ Microbiol 62:3861-3. 1996
    ..The accumulated trehalose is extracted from the bacteria by 70% hot ethanol and can easily be purified radiochemically by chromatographic techniques...
  31. pmc Characterization of the aes gene of Escherichia coli encoding an enzyme with esterase activity
    R Peist
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 179:7679-86. 1997
    ..Also, overproduction of MalT counteracted the Aes-dependent repression, indicating a direct interaction between MalT and Aes...
  32. ncbi request reprint Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS
    E Schneider
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Biol Chem 267:5148-54. 1992
    ..Downstream of malS, after a 513-base pair intergenic region, lies the convergently transcribed gene avtA, which codes for the alanine/valine transaminase...
  33. ncbi request reprint Facilitated diffusion of p-nitrophenyl-alpha-D-maltohexaoside through the outer membrane of Escherichia coli. Characterization of LamB as a specific and saturable channel for maltooligosaccharides
    S Freundlieb
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Biol Chem 263:314-20. 1988
    ..Using p-nitrophenyl-alpha-D-maltohexaoside as a substrate, we determined a Km of 3 microM and a Vmax of 0.14 mumol/min/mg of protein...
  34. pmc The malX malY operon of Escherichia coli encodes a novel enzyme II of the phosphotransferase system recognizing glucose and maltose and an enzyme abolishing the endogenous induction of the maltose system
    J Reidl
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Bacteriol 173:4862-76. 1991
    ..This is not the case in a malT(Con) strain that expresses the mal genes constitutively. We conclude that malY encodes an enzyme that degrades the inducer of the maltose system or prevents its synthesis...
  35. pmc MalY of Escherichia coli is an enzyme with the activity of a beta C-S lyase (cystathionase)
    E Zdych
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 177:5035-9. 1995
    ..Also, the overexpression of plasmid-encoded metC did not affect mal gene expression, nor did the deduced amino acid sequence of MetC show homology to that of MalY...
  36. ncbi request reprint Purification and characterization of the heterologously expressed trehalose/maltose ABC transporter complex of the hyperthermophilic archaeon Thermococcus litoralis
    G Greller
    Department of Biology, University of Konstanz, Germany
    Eur J Biochem 268:4011-8. 2001
    ..litoralis or the soluble engineered protein. The purified native TMBP was identified as a glycoprotein...
  37. ncbi request reprint The Tsr chemosensory transducer of Escherichia coli assembles into the cytoplasmic membrane via a SecA-dependent process
    J F Gebert
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Biol Chem 263:16652-60. 1988
    ..Moreover, assembly of the Tsr protein seems to be closely coupled to its synthesis...
  38. ncbi request reprint Maltose transacetylase of Escherichia coli. Mapping and cloning of its structural, gene, mac, and characterization of the enzyme as a dimer of identical polypeptides with a molecular weight of 20,000
    B Brand
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Biol Chem 266:14113-8. 1991
    ..55, 0.20, 0.07, 0.04. Maltotriose and other oligosaccharides were acetylated with 2% of the rate determined for glucose. The Km for glucose and maltose were 62 and 90 mM, and the Vmax was 0.20 and 0.11 mmol/min x mg enzyme, respectively...
  39. pmc Mapping and cloning of gldA, the structural gene of the Escherichia coli glycerol dehydrogenase
    V Truniger
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 176:1796-800. 1994
    ..gldA was cloned, and its position on the physical map of E. coli was determined. The expression of gldA was induced by hydroxyacetone under stationary-phase growth conditions...
  40. ncbi request reprint Glycerol uptake in Escherichia coli is sensitive to membrane lipid composition
    V Truniger
    Department of Biology, University of Konstanz, Germany
    Res Microbiol 144:565-74. 1993
    ..The mutants were more resistant to the hydrophobic antibiotic tetracycline, as well as to the membrane perturbants ethanol and dimethylsulphoxide and to the stress of low-osmolarity medium...
  41. ncbi request reprint The transmembrane topology of the sn-glycerol-3-phosphate permease of Escherichia coli analysed by phoA and lacZ protein fusions
    P Gott
    Department of Biology, University of Konstanz, FRG
    Mol Microbiol 2:655-63. 1988
    ..In contrast, we feel that the enzymatic activity of lacZ fusions is only a limited parameter for studying the topology of a complex membrane protein...
  42. pmc Trehalose transport and metabolism in Escherichia coli
    W Boos
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Bacteriol 172:3450-61. 1990
    ..The phenotype of this mutant indicated that trehalose-6-phosphate is the effective in vivo inducer of the system...
  43. pmc Molecular analysis of the glpFKX regions of Escherichia coli and Shigella flexneri
    V Truniger
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 174:6981-91. 1992
    ..Bacteriol. 170:2136-2142, 1988) originated from the 88-min region of the Escherichia coli chromosome and not, as reported, from the 7-min region and that the gene product identified as MvrA is in fact encoded by a gene distal to glpX...
  44. pmc Alignment of genes from the 9-minute region (araJ to tsx) of the Escherichia coli K-12 linkage map to the physical map
    J M Lucht
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 174:1709-10. 1992
  45. ncbi request reprint Crystallization and preliminary X-ray analysis of the trehalose/maltose ABC transporter MalFGK2 from Thermococcus litoralis
    A Schiefner
    Fachbereich Biologie, Universitat Konstanz, M656, D 78457 Konstanz, Germany
    Acta Crystallogr D Biol Crystallogr 58:2147-9. 2002
    ..The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 106.5, b = 150.5, c = 170.1 A, beta = 107.8 degrees. A native data set has been obtained at a resolution of 5 A...
  46. ncbi request reprint The ABC maltose transporter
    M Ehrmann
    Fakultat fur Biologie, Universitat Konstanz, Germany
    Mol Microbiol 29:685-94. 1998
    ..In addition, recent experimental evidence suggests that regulation of gene expression and transport activity is far more complex than expected...
  47. ncbi request reprint sn-Glycerol-3-phosphate transport in Escherichia coli and Salmonella typhimurium
    T Larson
    Tokai J Exp Clin Med 7:149-55. 1982
    ..The synthesis of the remaining polypeptide of 30,000 prevented the proper assembly of other transport related binding proteins such as the ribose- and galactose-binding protein...
  48. pmc X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression
    T Clausen
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, D 82152 Martinsried
    EMBO J 19:831-42. 2000
    ..Therefore, we propose that a direct protein-protein interaction with MalT, the central transcriptional activator of the maltose system, underlies MalY-dependent repression of the maltose system...
  49. ncbi request reprint The ATP-binding cassette subunit of the maltose transporter MalK antagonizes MalT, the activator of the Escherichia coli mal regulon
    C H Panagiotidis
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA
    Mol Microbiol 30:535-46. 1998
    ..This model suggests that bacteria may be able to couple information about extracellular substrate availability to the transcriptional apparatus via the levels of ATP hydrolysis associated with transport...
  50. ncbi request reprint Dependence of maltose transport and chemotaxis on the amount of maltose-binding protein
    M D Manson
    J Biol Chem 260:9727-33. 1985
    ..A half-maximal chemotactic response occurred at 25% of the malE+ MBP level, suggesting that in vivo the KD for binding of maltose-loaded MBP to Tar is quite high (approximately 250 microM)...
  51. ncbi request reprint A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors
    V Schreiber
    Unité de Génétique Moléculaire, URA CNRS 1773, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Mol Microbiol 35:765-76. 2000
    ..Together, these results establish that MalY acts directly upon MalT without the help of any factor, and that MalY is a negative effector of MalT competing with the inducer for MalT binding...
  52. ncbi request reprint Alpha-amylase of Escherichia coli, mapping and cloning of the structural gene, malS, and identification of its product as a periplasmic protein
    S Freundlieb
    J Biol Chem 261:2946-53. 1986
    ..These properties differentiate this periplasmic enzyme from the cytoplasmic amylomaltase and define it as an alpha-amylase...
  53. pmc Crystal structure of MalK, the ATPase subunit of the trehalose/maltose ABC transporter of the archaeon Thermococcus litoralis
    K Diederichs
    Fachbereich Biologie, Universitat Konstanz, M656, D 78457 Konstanz, Germany
    EMBO J 19:5951-61. 2000
    ..The structure and its relationship to function are discussed in the light of known mutations from the homologous Escherichia coli and Salmonella typhimurium proteins...
  54. pmc Identification of endogenous inducers of the mal regulon in Escherichia coli
    M Ehrmann
    J Bacteriol 169:3539-45. 1987
    ..coli linkage map. A Tn10 insertion in malI also resulted in the loss of constitutivity at low osmolarity and delayed the induction of the maltose regulon by exogenous inducers...
  55. ncbi request reprint Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system
    P Overduin
    Department of Molecular Cell Biology, State University of Utrecht, The Netherlands
    Mol Microbiol 2:767-75. 1988
    ..The sequences of the proteins contain the characteristics of several other binding protein-dependent transport systems, but they seem to be particularly closely related to the maltose system...
  56. pmc Osmoregulation of the maltose regulon in Escherichia coli
    B Bukau
    J Bacteriol 166:884-91. 1986
    ..The repression at high osmolarity no longer occurred in malQ mutants, however, provided that glycogen was present...
  57. ncbi request reprint Analysis and DNA sequence of the osmoregulated treA gene encoding the periplasmic trehalase of Escherichia coli K12
    C Gutierrez
    Centre de Recherche de Biochimie et de Genetique Cellulaire du C N R S, Toulouse, France
    Mol Gen Genet 217:347-54. 1989
    ....
  58. ncbi request reprint Comparison of sequences from the malB regions of Salmonella typhimurium and Enterobacter aerogenes with Escherichia coli K12: a potential new regulatory site in the interoperonic region
    M K Dahl
    Fachbereich für Biologie, Universitat Konstanz, Federal Republic of Germany
    Mol Gen Genet 218:199-207. 1989
    ..coli. The predicted amino acid sequence of the encoded proteins showed 90% or more identity in every pairwise comparison of species...
  59. pmc Identification of the glpT-encoded sn-glycerol-3-phosphate permease of Escherichia coli, an oligomeric integral membrane protein
    T J Larson
    J Bacteriol 152:1008-21. 1982
    ..The present report demonstrates that it is not the gene product of glpT and is not required for active transport of sn-glycerol-3-phosphate...
  60. ncbi request reprint Characterization of the structural requirements for assembly and nucleotide binding of an ATP-binding cassette transporter. The maltose transport system of Escherichia coli
    C H Panagiotidis
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    J Biol Chem 268:23685-96. 1993
    ..Excess MalK competed with the MalK-LacZ hybrid protein for sites in the membrane and resulted in the hybrid fractionating as a soluble protein.(ABSTRACT TRUNCATED AT 400 WORDS)..
  61. ncbi request reprint Only one gene is required for the glpT-dependent transport of sn-glycerol-3-phosphate in Escherichia coli
    D Ludtke
    Mol Gen Genet 186:540-7. 1982
    ..coli linkage map. Complementation analysis using these mutants revealed only one complementation group. Thus, one gene is necessary and sufficient for the proton motive force-dependent sn-glycerol-3-phosphate transport system...
  62. pmc Evolution of chemotactic-signal transducers in enteric bacteria
    M K Dahl
    Universitaet Konstanz, Department of Biology, Federal Republic of Germany
    J Bacteriol 171:2361-71. 1989
    ..These conserved regions include residues implicated in amino-acid binding...
  63. pmc Kinetic analysis by in vivo 31P nuclear magnetic resonance of internal Pi during the uptake of sn-glycerol-3-phosphate by the pho regulon-dependent Ugp system and the glp regulon-dependent GlpT system
    K B Xavier
    Instituto de Tecnologia Quimica e Biologica, UNL, Oeiras, Portugal
    J Bacteriol 177:699-704. 1995
    ..Both systems were inversely controlled by internal Pi. Whereas the Ugp system was inhibited, the GlpT system was stimulated by elevated internal Pi...
  64. ncbi request reprint cir, a gene conferring resistance to colicin I maps between mgl and fpk on the Escherichia coli chromosome
    W Boos
    Mol Gen Genet 191:401-6. 1983
    ..It is not regulated by D-fucose, the inducer of the mgl system and mutants defective in cir are unimpaired in the uptake of substrates of the mgl-dependent transport system...
  65. pmc Divergent transcription of the sn-glycerol-3-phosphate active transport (glpT) and anaerobic sn-glycerol-3-phosphate dehydrogenase (glpA glpC glpB) genes of Escherichia coli K-12
    M Ehrmann
    J Bacteriol 169:526-32. 1987
    ..These three genes were present in an operon transcribed in the order glpA glpC glpB in the clockwise direction on the linkage map of E. coli...
  66. ncbi request reprint Mapping of markers in the gyrA-his region of Escherichia coli
    A Middendorf
    Mol Gen Genet 197:175-81. 1984
    ..We conclude that our previously published sequence of markers was incorrect and is in fact inverted. The present data indicate the following sequence of markers in clockwise order: his gat cdd mgl cir fpk gyrA...
  67. ncbi request reprint Characterization of two genes, glpQ and ugpQ, encoding glycerophosphoryl diester phosphodiesterases of Escherichia coli
    J Tommassen
    Department of Molecular Cell Biology, University of Utrecht, The Netherlands
    Mol Gen Genet 226:321-7. 1991
    ..e., 46 of the 146 amino acids are Gln or Asn. This polypeptide and the UgpQ protein were identified in an in vitro transcription/translation system as proteins with apparent molecular weights of 19.5 and 27 kDa, respectively...
  68. ncbi request reprint Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phosphate transport protein with components of the hexose-6-phosphate transport system
    K Eiglmeier
    Fakultat fur Biologie, Universitat Konstanz, FRG
    Mol Microbiol 1:251-8. 1987
    ..These findings indicate how catabolite repression may be mediated but do not explain its physiological significance in glycerol metabolism...
  69. ncbi request reprint Characterization of the ugp region containing the genes for the phoB dependent sn-glycerol-3-phosphate transport system of Escherichia coli
    H Schweizer
    Mol Gen Genet 197:161-8. 1984
    ..No gene products could be assigned to the ugpA and ugpD genes. Hybridization experiments allowed the physical characterization of 20 kb of DNA adjacent to the ugp genes on the E. coli chromosome including the liv genes...
  70. ncbi request reprint Cloning of the ugp region containing the structural genes for the pho regulon-dependent sn-glycerol-3-phosphate transport system of Escherichia coli
    H Schweizer
    Mol Gen Genet 192:177-86. 1983
    ..Using ugp-lacZ fusions, it could be shown that the ugp region consists of at least two different operons that are transcribed in the same direction (counterclockwise) on the E. coli chromosome...
  71. pmc Repressor for the sn-glycerol-3-phosphate regulon of Escherichia coli K-12: cloning of the glpR gene and identification of its product
    H Schweizer
    J Bacteriol 161:563-6. 1985
    ..Analysis of proteins labeled in minicells harboring either glpR+ recombinant plasmids or a glpR::Tn5 derivative showed that the glpR gene product is a protein with an apparent molecular weight of 33,000...
  72. ncbi request reprint Periplasmic glycerophosphodiester phosphodiesterase of Escherichia coli, a new enzyme of the glp regulon
    T J Larson
    J Biol Chem 258:5428-32. 1983
    ..The substrates of the phosphodiesterase had no affinity for the glpT-encoded active transport system. Thus, the glpQ gene product expands the catabolic capability of the glp regulon to include a variety of glycerophosphodiesters...
  73. pmc Mapping of two ugp genes coding for the pho regulon-dependent sn-glycerol-3-phosphate transport system of Escherichia coli
    H Schweizer
    J Bacteriol 150:1164-71. 1982
    ..It is either the structural gene for the binding protein or located proximal to it. The analysis of the crosses allowed the ordering of the markers in the clockwise direction as follows: aroB, malA, asd, ugpA, ugpB, livH, pit...