Research Topics
Genomes and GenesSpecies | Peter B BeckerSummaryAffiliation: University of Munich Country: Germany Publications
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Publications
Cumulative contributions of weak DNA determinants to targeting the Drosophila dosage compensation complexGregor D Gilfillan
Adolf Butenandt Institut, Molekularbiologie, Ludwig Maximilian Universität, Schillerstrasse 44, 80336 Munich, Germany
Nucleic Acids Res 35:3561-72. 2007..The results support a model for MSL2 recruitment according to which several different, degenerate sequence motifs of variable affinity cluster and synergise to form a high affinity site...- Nucleosome sliding: facts and fictionPeter B Becker
Adolf Butenandt Institut, Molekularbiologie, Ludwig Maximilians Universitat, D 80336 MUnchen, Germany
EMBO J 21:4749-53. 2002..This review discusses the possible roles for nucleosome sliding in the assembly and maintenance of dynamic chromatin and for the regulation of diverse functions in eukaryotic nuclei... 
ATP-dependent nucleosome remodelingPeter B Becker
Adolf Butenandt Institut, Molekularbiologie, 80336 Munich, Germany
Annu Rev Biochem 71:247-73. 2002....
The activation potential of MOF is constrained for dosage compensationMatthias Prestel
Adolf Butenandt Institute and Centre for Integrated Protein Science, Ludwig Maximilians University, 80336 Munich, Germany
Mol Cell 38:815-26. 2010....- Site-specific acetylation of ISWI by GCN5Roger Ferreira
Adolf Butenandt Institut, Molekularbiologie, 80336 Munchen, Germany
BMC Mol Biol 8:73. 2007..The diversity of nucleosome remodelling factors is reflected by a multitude of ATPase complexes with distinct subunit composition... - Lifting a chromosome: dosage compensation in Drosophila melanogasterGregor D Gilfillan
Adolf Butenandt Institut, Molekularbiologie Schillerstrasse 44, 80336 Munich, Germany
FEBS Lett 567:8-14. 2004..Deciphering the principles of X chromosome recognition and the nature of the chromatin configuration, that allows fine-tuning of transcription, remain the most interesting challenges... - The DNA binding CXC domain of MSL2 is required for faithful targeting the Dosage Compensation Complex to the X chromosomeTorsten Fauth
Adolf Butenandt Institute and Centre for Integrated Protein Science, Ludwig Maximilians University, 80336 Munich, Germany
Nucleic Acids Res 38:3209-21. 2010..Reporter gene assays and localization of GFP-fusion proteins confirm the important contribution of the CXC domain for DCC targeting in vivo... - The MRG domain mediates the functional integration of MSL3 into the dosage compensation complexViolette Morales
Adolf Butenandt Institut, Molekularbiologie, Schillerstr 44, 80336 Munchen, Germany
Mol Cell Biol 25:5947-54. 2005..An N-terminal truncation that lacks the chromo-related domain and all nucleic acid binding activity is able to trigger de novo assembly of the DCC and establishment of an acetylated X-chromosome territory... - Drosophila ISWI regulates the association of histone H1 with interphase chromosomes in vivoGiorgia Siriaco
Adolf Butenandt Institute, Molecular Biology, Munich Center of Integrated Protein Science, Ludwig Maximilians University, 80336 Munich, Germany
Genetics 182:661-9. 2009..Our findings suggest that ISWI regulates higher-order chromatin structure by modulating the interaction of H1 with interphase chromosomes... - Dosage compensation: the beginning and end of generalizationTobias Straub
Adolf Butenandt Institute, Department of Molecular Biology, Ludwig Maximilians University, 80336 Munich, Germany
Nat Rev Genet 8:47-57. 2007.... - Functional integration of the histone acetyltransferase MOF into the dosage compensation complexViolette Morales
Adolf Butenandt Institut, Molekularbiologie, Munich, Germany
EMBO J 23:2258-68. 2004..Activation of MOF's HAT activity upon integration into the DCC may serve to restrict the critical histone modification to the male X chromosome... - Targeting determinants of dosage compensation in DrosophilaIna K Dahlsveen
Adolf Butenandt Institut, Molekularbiologie, Ludwig Maximilians Universitat Munchen, Munich, Germany
PLoS Genet 2:e5. 2006..Our bioinformatics analysis suggests that DCC binding sites may be composed of variable combinations of degenerate motifs... - The ATPase domain of ISWI is an autonomous nucleosome remodeling machineFelix Mueller-Planitz
Adolf Butenandt Institut, Ludwig Maximilians Universitat, Munich, Germany
Nat Struct Mol Biol 20:82-9. 2013..Nucleosome-remodeling enzymes may thus have evolved directly from ancestral helicase-type motors, and peripheral domains have furnished regulatory capabilities that bias the remodeling reaction toward different structural outcomes... - The dosage compensation complex shapes the conformation of the X chromosome in DrosophilaCharlotte Grimaud
Adolf Butenandt Institute and Centre for Integrated Protein Science CiPSM, Ludwig Maximilians University, 80336 Munich, Germany
Genes Dev 23:2490-5. 2009..We propose that nuclear organization of HAS is central to the faithful distribution of the DCC along the X chromosome... - Chromosome-wide gene-specific targeting of the Drosophila dosage compensation complexGregor D Gilfillan
Adolf-Butenandt-Institut, Molekularbiologie, , , Germany
Genes Dev 20:858-70. 2006..Within the targeted genes, the DCC exhibits a strong preference for exons and coding sequences. Our results demonstrate gene-specific binding of the DCC, and identify several sequence elements that may partly direct its targeting... - Dosage compensation in flies: mechanism, models, mysteryTobias Straub
Adolf Butenandt Institut, Molekularbiologie, Schillerstrasse 44, 80336 Munchen, Germany
FEBS Lett 579:3258-63. 2005..We discuss the prevalent models in the context of recent experimental observations... - ACF1 improves the effectiveness of nucleosome mobilization by ISWI through PHD-histone contactsAnton Eberharter
Adolf Butenandt Institut, Molekularbiologie, Munchen, Germany
EMBO J 23:4029-39. 2004..Connecting ACF to histones may be prerequisite for efficient conversion of ATP-dependent conformational changes of ISWI into translocation of DNA relative to the histones during nucleosome mobilization... - The Drosophila MSL complex activates the transcription of target genesTobias Straub
Adolf Butenandt-Institute, Molecular Biology Unit, Ludwig-Maximilians-University, 80336 Munich, Germany
Genes Dev 19:2284-8. 2005..The results unequivocally document an approximate twofold activation of target genes by the MSL complex... - ACF catalyses chromatosome movements in chromatin fibresVerena K Maier
Molekularbiologie, Adolf Butenandt Institut, Ludwig Maximilian Universität, Munich, Germany
EMBO J 27:817-26. 2008..This first demonstration of catalysed chromatosome movements suggests that the bulk of interphase euchromatin may be rendered dynamic by dedicated nucleosome-remodelling factors... - Nucleosome remodeling: one mechanism, many phenomena?Gernot Längst
Adolf Butenandt Institut, Molekularbiologie, Ludwig Maximilians University Munchen, Schillerstrasse 44, D 80336 Munich, Germany
Biochim Biophys Acta 1677:58-63. 2004..This review explores the alternative possibility that the rich phenomenology of nucleosome remodeling may be brought about by variations of one basic remodeling reaction... - A nucleosome sliding assay for chromatin remodeling factorsAnton Eberharter
Adolf-Butenandt-Institut, Molekularbiologie, , Germany
Methods Enzymol 377:344-53. 2004 - The DNA chaperone HMGB1 facilitates ACF/CHRAC-dependent nucleosome slidingTiziana Bonaldi
Adolf Butenandt Institut, Molekularbiologie, Schillerstrasse 44, D 80336 MUnchen, Germany
EMBO J 21:6865-73. 2002..By contrast, an HMGB1 mutant with increased binding affinity was inhibitory. These observations are consistent with a role for HMGB1 as a DNA chaperone facilitating the rate-limiting DNA distortion during nucleosome remodelling... - The dMi-2 chromodomains are DNA binding modules important for ATP-dependent nucleosome mobilizationKarim Bouazoune
Adolf Butenandt Institut, Molekularbiologie, Ludwig Maximilians Universitat, Schillerstrasse 44, D 80336 MUnchen, Germany
EMBO J 21:2430-40. 2002..Furthermore, we identify DNA binding as a novel chromodomain-associated activity... - ATP-dependent nucleosome remodelling: factors and functionsAnton Eberharter
Adolf-Butenandt-Institut, Molekularbiologie, Schillerstr. 44, , Germany
J Cell Sci 117:3707-11. 2004 - MSL2 combines sensor and effector functions in homeostatic control of the Drosophila dosage compensation machineryRaffaella Villa
Molecular Biology Unit, Adolf Butenandt Institute and Center for Integrated Protein Science, Ludwig Maximilians University, 80336 Munich, Germany
Mol Cell 48:647-54. 2012..Furthermore, systematic mapping of modification sites by mass spectrometry and chromatin interaction studies on the target protein MSL1 suggest that the role of MSL2-mediated ubiquitylation goes beyond proteolysis... - The many colours of chromodomainsAlexander Brehm
Adolf Butenandt Institut, Molekularbiologie, Munchen, Germany
Bioessays 26:133-40. 2004.... - ATP-dependent chromatosome remodelingVerena K Maier
Adolf Butenandt Institut, Abt Molekularbiologie, and Münchner Zentrum für Integrierte Proteinforschung, Ludwig Maximilian Universitat Munchen, Schillerstrasse 44, D 80336 MUnchen, Germany
Biol Chem 389:345-52. 2008..Recent findings suggest that some remodeling machines can reorganize even folded chromatin fibers containing the linker histone H1, extending the potential scope of remodeling reactions to the bulk of euchromatin... - Transcription modulation chromosome-wide: universal features and principles of dosage compensation in worms and fliesTobias Straub
Adolf Butenandt Institut and Center for Integrated Protein Science Munich CIPSM, Ludwig Maximilians Universitat, Schillerstrasse 44, D 80336 Munich, Germany
Curr Opin Genet Dev 21:147-53. 2011.... - Histone acetylation: a switch between repressive and permissive chromatin. Second in review series on chromatin dynamicsAnton Eberharter
Adolf-Butenandt-Institut, Molekularbiologie, , Schillerstrasse 44, , Germany
EMBO Rep 3:224-9. 2002..The targeting of enzymes that modulate the histone acetylation status of chromatin, in synergy with the effects mediated by other chromatin remodeling factors, is central to gene regulation... - Preparation of chromatin assembly extracts from preblastoderm Drosophila embryosEdgar Bonte
Molekularbiologie, Adolf Butenandt Institut, Munchen, Germany
Methods Mol Biol 523:1-10. 2009..The resulting chromatin is an excellent approximation of physiological chromatin in vivo. This chapter describes the preparation of chromatin assembly extracts and the chromatin assembly reaction... - DNA sequence and the organization of chromosomal domainsTobias Straub
Adolf Butenandt Institut and Center for Integrated Protein Science Munich, Ludwig Maximilian Universität, Schillerstrasse 44, D 80336 Munich, Germany
Curr Opin Genet Dev 18:175-80. 2008..Inspiration and motivation for this discourse comes from the unresolved question of how X chromosomes are identified for dosage compensation... - Nucleosome dynamics and epigenetic stabilityPhilipp Korber
Adolf Butenandt Institute, Ludwig Maximilians University, 80336 Munich, Germany
Essays Biochem 48:63-74. 2010..All of them may contribute to the assembly of heritable epigenetic structures... - Dosage compensation and the global re-balancing of aneuploid genomesMatthias Prestel
Adolf Butenandt Institute and Centre for Integrated Protein Science CiPSM, Ludwig Maximilians University, Schillerstrasse 44, 80336 Munich, Germany
Genome Biol 11:216. 2010..The dosage-compensation systems that evolved along with monosomic sex chromosomes exemplify the intricacies of compensating for differences in gene copy number by transcriptional regulation... - Gene regulation by histone H1: new links to DNA methylationRalph A W Rupp
Adolf-Butenandt-Institut, Molekularbiologie, , Germany
Cell 123:1178-9. 2005..Although a 50% reduction of histone H1 levels in embryonic stem cells affects chromatin structure globally, the expression of very few genes is altered. Intriguingly, this study reveals a new link between H1 and DNA methylation... - A critical epitope for substrate recognition by the nucleosome remodeling ATPase ISWICedric R Clapier
Adolf Butenandt Institut, Molekularbiologie, Ludwig Maximilians Universitat Munchen, Schillerstrasse 44, 80336 Munchen, Germany
Nucleic Acids Res 30:649-55. 2002..Consistent with this hypothesis, the ISWI ATPase is stimulated by isolated H4 tail peptides ISWI only in the presence of DNA. Acetylation of the adjacent K12 and K16 residues impairs substrate recognition by ISWI... - Wolfram Hörz 1944-2005Peter B Becker
Adolf-Butenandt-Institut, Molekularbiologie, , Germany
Cell 124:13-4. 2006 - Recruitment of the nucleolar remodeling complex NoRC establishes ribosomal DNA silencing in chromatinRalf Strohner
Adolf-Butenandt-Institut, Molekularbiologie, , 80336 Munich. German
Mol Cell Biol 24:1791-8. 2004..These results reveal an important role for TTF-I in recruiting NoRC to rDNA and an active role for NoRC in the establishment of rDNA silencing... - Analysis of activator-dependent transcription reinitiation in vitroRaphael Sandaltzopoulos
Department of Molecular Biology, Democritus University of Thrace, University Hospital at Dragana, G-68100 Alexandroupolis, Greece
Methods Enzymol 370:487-501. 2003 - Gene regulation: a finger on the markPeter B Becker
Nature 442:31-2. 2006 - Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWITim Grüne
European Molecular Biology Laboratory, Grenoble Outstation, B P 181, F 38042 Grenoble 9, France
Mol Cell 12:449-60. 2003..An integrated structural and functional analysis of these domains provides insight into how ISWI interacts with the nucleosomal substrate... - Sequence-specific targeting of Drosophila roX genes by the MSL dosage compensation complexYongkyu Park
Howard Hughes Medical Institute, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Mol Cell 11:977-86. 2003..Our results are consistent with a model in which nascent roX RNA assembly with MSL proteins is an early step in the initiation of dosage compensation... - Ran binds to chromatin by two distinct mechanismsDaniel Bilbao-Cortes
European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Curr Biol 12:1151-6. 2002..We propose that the Ran binding capacity of chromatin contributes to localized spindle and NE assembly... - The histone fold subunits of Drosophila CHRAC facilitate nucleosome sliding through dynamic DNA interactionsKlaus F Hartlepp
European Molecular Biology Laboratory, Grenoble Outstation, France
Mol Cell Biol 25:9886-96. 2005.... - Nucleosome binding by the bromodomain and PHD finger of the transcriptional cofactor p300Anja Ragvin
Department of Molecular Biology, University of Bergen, HIB, , N-5020 Bergen, Norway
J Mol Biol 337:773-88. 2004..Our data are consistent with a model where the two domains cooperate in nucleosome binding. In this model, both the bromodomain and the PHD finger contact the nucleosome while simultaneously interacting with each other... - Modulation of ISWI function by site-specific histone acetylationDavide F V Corona
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA
EMBO Rep 3:242-7. 2002..These findings suggest that H4K16 acetylation directly counteracts chromatin compaction mediated by the ISWI ATPase... - Stable chromosomal association of MSL2 defines a dosage-compensated nuclear compartmentTobias Straub
Department of Molecular Biology, Adolf Butenandt Institute, Schillerstr 44, 80336, Munich, Germany
Chromosoma 114:352-64. 2005..Our findings have profound implications for the mechanism underlying dosage compensation and furthermore provide a new, conceptual reference of stability in an otherwise highly dynamic nuclear environment... - Structure-function analysis of the RNA helicase malelessAnnalisa Izzo
Adolf Butenandt Institut and Center of integrated Protein Science, Munchen, Germany
Nucleic Acids Res 36:950-62. 2008..The C-terminal domain containing a glycine-rich heptad repeat adds potential dimerization and RNA-binding surfaces which are not required for helicase activity... - Combined use of RNAi and quantitative proteomics to study gene function in DrosophilaTiziana Bonaldi
Max Planck Institute of Biochemistry, Department of Proteomics and Signal Transduction, Am Klopferspitz 18, D 82152 Martinsried, Germany
Mol Cell 31:762-72. 2008..We found little overall correlation between changes in the transcriptome and proteome with many protein changes unaccompanied by message changes. However, correlation was high for those mRNAs that changed significantly by microarray... - Acetylation increases access of remodelling complexes to their nucleosome targets to enhance initiation of V(D)J recombinationKarl P Nightingale
Institute of Biomedical Research, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
Nucleic Acids Res 35:6311-21. 2007.... - Regulation of higher-order chromatin structures by nucleosome-remodelling factorsPatrick D Varga-Weisz
Babraham Institute, Cambridge, CB2 4AT, UK
Curr Opin Genet Dev 16:151-6. 2006..Recent observations support roles for nucleosome-remodelling factors at the supra-nucleosomal level... - Nucleosome remodelers on trackPeter B Becker
Nat Struct Mol Biol 12:732-3. 2005