Annette G Beck-Sickinger

Summary

Affiliation: University of Leipzig
Country: Germany

Publications

  1. ncbi request reprint Structure-activity relationship studies: methods and ligand design for G-protein coupled peptide receptors
    Manja Lang
    Institute of Biochemistry, University of Leipzig, Germany
    Curr Protein Pept Sci 7:335-53. 2006
  2. ncbi request reprint Identification of an efficacy switch region in the ghrelin receptor responsible for interchange between agonism and inverse agonism
    Birgitte Holst
    Laboratory for Molecular Pharmacology, The Panum Institute, Blegdamsvej 3, University of Copenhagen, 2200 Copenhagen N, Denmark
    J Biol Chem 282:15799-811. 2007
  3. doi request reprint Novel chemically modified analogues of neuropeptide Y for tumor targeting
    Denise Zwanziger
    Institute of Biochemistry, Leipzig University, Bruderstr 34, 04103 Leipzig, Germany
    Bioconjug Chem 19:1430-8. 2008
  4. doi request reprint Palmitoylated SDF1α shows increased resistance against proteolytic degradation in liver homogenates
    Kathrin Bellmann-Sickert
    Institute of Biochemistry, Leipzig University, Germany
    ChemMedChem 6:193-200. 2011
  5. doi request reprint Genetically encoded photocrosslinkers as molecular probes to study G-protein-coupled receptors (GPCRs)
    Annette G Beck-Sickinger
    AK Biochemie und Biooranische Chemie, Institut fur Biochemie, Universitat Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    Angew Chem Int Ed Engl 51:310-2. 2012
  6. pmc Illuminating the life of GPCRs
    Ilka Böhme
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Brüderstr, 34, 04103 Leipzig, Germany
    Cell Commun Signal 7:16. 2009
  7. doi request reprint Dimerization of adiponectin receptor 1 is inhibited by adiponectin
    David Kosel
    Faculty of Biosciences, Institute of Biochemistry, Leipzig University, Bruderstr 34, 04103 Leipzig, Germany
    J Cell Sci 123:1320-8. 2010
  8. ncbi request reprint Localization of novel adiponectin receptor constructs
    Cornelia M Deckert
    Faculty of Biosciences, Institute of Biochemistry, Pharmacy and Psychology, University of Leipzig, Leipzig, Germany
    J Recept Signal Transduct Res 26:647-57. 2006
  9. doi request reprint The interaction of neuropeptide Y with negatively charged and zwitterionic phospholipid membranes
    Lars Thomas
    Institute of Medical Physics and Biophysics, University of Leipzig, Leipzig, Germany
    Eur Biophys J 38:663-77. 2009
  10. pmc Vaspin inhibits kallikrein 7 by serpin mechanism
    John T Heiker
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Universitat Leipzig, Brüderstraße 34, Leipzig, Germany
    Cell Mol Life Sci 70:2569-83. 2013

Collaborators

Detail Information

Publications95

  1. ncbi request reprint Structure-activity relationship studies: methods and ligand design for G-protein coupled peptide receptors
    Manja Lang
    Institute of Biochemistry, University of Leipzig, Germany
    Curr Protein Pept Sci 7:335-53. 2006
    ..Furthermore we provide an insight into peptide based drug design. Examples are taken from the field of CGRP, orexin and growth hormone secretagogue receptor ligands...
  2. ncbi request reprint Identification of an efficacy switch region in the ghrelin receptor responsible for interchange between agonism and inverse agonism
    Birgitte Holst
    Laboratory for Molecular Pharmacology, The Panum Institute, Blegdamsvej 3, University of Copenhagen, 2200 Copenhagen N, Denmark
    J Biol Chem 282:15799-811. 2007
    ..e. in a more profound binding mode. This relationship between different binding modes and opposite efficacy is in accordance with the Global Toggle Switch model for 7TM receptor activation...
  3. doi request reprint Novel chemically modified analogues of neuropeptide Y for tumor targeting
    Denise Zwanziger
    Institute of Biochemistry, Leipzig University, Bruderstr 34, 04103 Leipzig, Germany
    Bioconjug Chem 19:1430-8. 2008
    ..In vitro and in vivo studies suggest that receptor-selective analogues of NPY have promising characteristics for future applications in nuclear medicine for breast tumor diagnosis and therapy...
  4. doi request reprint Palmitoylated SDF1α shows increased resistance against proteolytic degradation in liver homogenates
    Kathrin Bellmann-Sickert
    Institute of Biochemistry, Leipzig University, Germany
    ChemMedChem 6:193-200. 2011
    ..This study is a proof of principle approach for the lipidation of SDF1α and provides a basis for further engineering of the chemokine in order to increase its therapeutic value...
  5. doi request reprint Genetically encoded photocrosslinkers as molecular probes to study G-protein-coupled receptors (GPCRs)
    Annette G Beck-Sickinger
    AK Biochemie und Biooranische Chemie, Institut fur Biochemie, Universitat Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    Angew Chem Int Ed Engl 51:310-2. 2012
    ..In this way the receptor surface could be mapped by searching for specific ligand interaction sites and the complex dynamics could be studied. This method is also useful for probing the structure of GPCR complexes in living cells...
  6. pmc Illuminating the life of GPCRs
    Ilka Böhme
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Brüderstr, 34, 04103 Leipzig, Germany
    Cell Commun Signal 7:16. 2009
    ..Fluorescent ligands, antibodies, auto-fluorescent proteins as well as the evolving technologies for chemical labeling with peptide- and protein-tags are described and their major applications concerning the GPCR life cycle are presented...
  7. doi request reprint Dimerization of adiponectin receptor 1 is inhibited by adiponectin
    David Kosel
    Faculty of Biosciences, Institute of Biochemistry, Leipzig University, Bruderstr 34, 04103 Leipzig, Germany
    J Cell Sci 123:1320-8. 2010
    ..Accordingly, this is the first direct read-out signal of adiponectin at the AdipoR1 receptor, which revealed the involvement of specific amino acids of both the receptor and the ligand to modulate the quaternary structure of the AdipoR1...
  8. ncbi request reprint Localization of novel adiponectin receptor constructs
    Cornelia M Deckert
    Faculty of Biosciences, Institute of Biochemistry, Pharmacy and Psychology, University of Leipzig, Leipzig, Germany
    J Recept Signal Transduct Res 26:647-57. 2006
    ..In parallel, comparative experiments were performed with the NPY Y2-receptor, a classical rhodopsin-like GPCR...
  9. doi request reprint The interaction of neuropeptide Y with negatively charged and zwitterionic phospholipid membranes
    Lars Thomas
    Institute of Medical Physics and Biophysics, University of Leipzig, Leipzig, Germany
    Eur Biophys J 38:663-77. 2009
    ..For membranes composed of phosphatidylcholine and phosphatidylserine, an intermediate position of the alpha-helix is observed...
  10. pmc Vaspin inhibits kallikrein 7 by serpin mechanism
    John T Heiker
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Universitat Leipzig, Brüderstraße 34, Leipzig, Germany
    Cell Mol Life Sci 70:2569-83. 2013
    ....
  11. pmc Selective mode of action of guanidine-containing non-peptides at human NPFF receptors
    Maria Findeisen
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Bruderstrasse 34, D 04103 Leipzig, Germany
    J Med Chem 55:6124-36. 2012
    ..35 in the NPFF(1) but not in the NPFF(2) receptor. These results provide distinct insights into the receptor specific binding pockets, which is necessary for the development of drugs to address the NPFF system...
  12. doi request reprint A reconstitution protocol for the in vitro folded human G protein-coupled Y2 receptor into lipid environment
    Peter Schmidt
    Institute of Medical Physics and Biophysics, University of Leipzig, Hartelstr 16 18, D 04107 Leipzig, Germany
    Biophys Chem 150:29-36. 2010
    ..The efficiency of the reconstitution could also be inferred from the fact that reasonable (13)C NMR spectra at natural abundance could be acquired...
  13. doi request reprint Artificial chemokines: combining chemistry and molecular biology for the elucidation of interleukin-8 functionality
    Ralf David
    Institut fur Biochemie, Fakultat fur Biowissenschaften, Pharmazie and Psychologie, Universitat Leipzig, Bruderstrasse 34, D 04103 Leipzig, Germany
    J Am Chem Soc 130:15311-7. 2008
    ..Concepts like this could be a first step toward the synthesis of proteins consisting of large artificial secondary structure elements...
  14. ncbi request reprint Receptor subtype-specific docking of Asp6.59 with C-terminal arginine residues in Y receptor ligands
    Nicole Merten
    Institute of Biochemistry, Faculty of Bioscience, Pharmacy and Psychology, University of Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    J Biol Chem 282:7543-51. 2007
    ..Furthermore, the proposed model of a subtype-specific binding mode is in agreement with the evolution of YRs...
  15. doi request reprint GPC receptors and not ligands decide the binding mode in neuropeptide Y multireceptor/multiligand system
    Diana Lindner
    Leipzig University, Institute of Biochemistry, Bruderstrasse 34, 04103 Leipzig, Germany
    Biochemistry 47:5905-14. 2008
    ..We can show that most of the residues identified to be critical for ligand binding are located within the now postulated binding pocket...
  16. doi request reprint Residue 75 of interleukin-8 is crucial for its interactions with glycosaminoglycans
    Karoline Nordsieck
    Institute of Biochemistry, Universitat Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    Chembiochem 13:2558-66. 2012
    ..These findings indicate that the N terminus of the E75K variant is more important as a second binding site for GAGs than that of the wild-type IL-8(1-77)...
  17. pmc Ligand-mimicking receptor variant discloses binding and activation mode of prolactin-releasing peptide
    Daniel Rathmann
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy, and Psychology, Universitat Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    J Biol Chem 287:32181-94. 2012
    ..This contributes to the knowledge of the binding of peptide ligands to GPCRs and might facilitate the development of GPCR ligands, but it also provides new targeting of CAMs involved in hereditary diseases...
  18. doi request reprint Generation of carrier peptides for the delivery of nucleic acid drugs in primary cells
    Robert Rennert
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Bruderstr 34, 04103 Leipzig, Germany
    ChemMedChem 3:241-53. 2008
    ....
  19. doi request reprint Neuropeptide Y receptors: ligand binding and trafficking suggest novel approaches in drug development
    Cornelia Walther
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Leipzig, Germany
    J Pept Sci 17:233-46. 2011
    ..We further address recent advances in YR-based drug design, the development of promising future drug candidates and novel approaches in YR-targeted tumor diagnostics and therapy opportunities...
  20. doi request reprint Assessment of a fully active class A G protein-coupled receptor isolated from in vitro folding
    Mathias Bosse
    Institute of Medical Physics and Biophysics, University of Leipzig, Hartelstr 16 18, D 04107 Leipzig, Germany
    Biochemistry 50:9817-25. 2011
    ..Thus, a very homogeneous sample of the Y2 receptor could be prepared that exhibited a K(D) value of 0.1 ± 0.05 nM for the binding of polypeptide Y, which represents one of the natural ligands of the Y2 receptor...
  21. pmc Characterization of the interaction of interleukin-8 with hyaluronan, chondroitin sulfate, dermatan sulfate and their sulfated derivatives by spectroscopy and molecular modeling
    Annelie Pichert
    Institute of Medical Physics and Biophysics, University of Leipzig, Hartelstr 16 18, D 04107 Leipzig, Germany
    Glycobiology 22:134-45. 2012
    ..Our results highlight the importance of combining experimental and theoretical approaches to obtain a better understanding of the molecular recognition properties of GAG-protein systems...
  22. ncbi request reprint From transcription profile to expression: the signaling repertoire of the SK-N-MC neuroepithelioma cell-line
    Jurgen E Bader
    Faculty of Biosciences, Pharmacy and Psychology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany
    J Recept Signal Transduct Res 24:257-82. 2004
    ..Protein expression was assayed with immunoblots by using antibodies directed against selected Galpha protein subunits. The presence of at least Galphas, Galphai3, and Galphai2 subunits was indicated...
  23. ncbi request reprint Structural properties of orexins for activation of their receptors
    Manja Lang
    Institute of Biochemistry, University of Leipzig, Germany
    J Pept Sci 12:258-66. 2006
    ..Additionally, we showed that no particular secondary structure is required for receptor subtype selectivity...
  24. doi request reprint Synthesis and application of peptides as drug carriers
    Robert Rennert
    Leipzig University, Leipzig, Germany
    Methods Mol Biol 535:389-403. 2009
    ..Both peptides were found to efficiently shuttle covalently linked small molecules and non-covalently complexed DNA and RNA inside human embryonic kidney cells (HEK 293)...
  25. ncbi request reprint Semisynthesis and application of carboxyfluorescein-labelled biologically active human interleukin-8
    Ralf David
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, D 04103 Leipzig, Germany
    Biol Chem 384:1619-30. 2003
    ..Thus, we introduce here a powerful approach that allows the site-specific incorporation of chemical modifications into the sequence of chemokines, which opens new avenues for studying IL-8 function...
  26. doi request reprint Agonist induced receptor internalization of neuropeptide Y receptor subtypes depends on third intracellular loop and C-terminus
    Ilka Böhme
    Institute of Biochemistry, Leipzig University, Brüderstr, 34, D 04103 Leipzig, Germany
    Cell Signal 20:1740-9. 2008
    ..By using novel hY(5)/hY(2) receptor chimera the receptor subtype dependent differences in hY receptor internalization could be identified on a molecular level...
  27. doi request reprint First selective agonist of the neuropeptide Y1-receptor with reduced size
    Denise Zwanziger
    Institute of Biochemistry, Leipzig University, Bruderstr 34, 04103 Leipzig, Germany
    J Pept Sci 15:856-66. 2009
    ..For the first time, here we show the design and characterization of a small Y(1)-receptor selective agonist. This agonist might be a useful novel ligand for NPY-mediated tumour diagnostics and therapeutics...
  28. doi request reprint Biocompatible silicon surfaces through orthogonal click chemistries and a high affinity silicon oxide binding peptide
    Rayk Hassert
    Institut fur Biochemie, Faculty of Biosciences, Pharmacy and Psychology, Universitat Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    Bioconjug Chem 23:2129-37. 2012
    ..The resulting peptide displayed an outstanding binding to silicon oxide and induced a significant increase in cell spreading and cell viability of osteoblasts on the oxidized silicon surface...
  29. ncbi request reprint Identification of the dimerisation interface of human interleukin-8 by IL-8-variants containing the photoactivatable amino acid benzoyl-phenylalanine
    Ralf David
    Institute of Biochemistry, Faculty of Bioscience, Pharmacy and Psychology, University of Leipzig, Leipzig, Germany
    Eur Biophys J 36:385-91. 2007
    ..Bpa(74)]hIL-8 has a high tendency to form covalent dimers whereas no dimer formation was observed for the variant with Bpa at position 65. Accordingly one residue of the dimerisation interface could be identified...
  30. ncbi request reprint Identification of the key residue of calcitonin gene related peptide (CGRP) 27-37 to obtain antagonists with picomolar affinity at the CGRP receptor
    Manja Lang
    Institute of Biochemistry, University of Leipzig, D 04103 Leipzig, Germany
    J Med Chem 49:616-24. 2006
    ..All high-affinity analogues showed antagonistic properties with potency similar to CGRP 8-37...
  31. pmc Ligand-induced internalization and recycling of the human neuropeptide Y2 receptor is regulated by its carboxyl-terminal tail
    Cornelia Walther
    Faculty of Biosciences, Institute of Biochemistry, Leipzig University, Bruderstrasse 34, 04103 Leipzig, Germany
    J Biol Chem 285:41578-90. 2010
    ..Our findings provide novel insights into complex mechanisms of controlled internalization of hY(2)R, which is likely applicable to other GPCRs...
  32. doi request reprint Towards understanding the free and receptor bound conformation of neuropeptide Y by fluorescence resonance energy transfer studies
    Michael Haack
    Institute of Biochemistry, Faculty of Bioscience, Pharmacy and Psychology, Leipzig University, Germany
    Chem Biol Drug Des 73:573-83. 2009
    ..However, the studies do not support a pancreatic polypeptide-like folding of neuropeptide Y in the presence of membranes that express the human Y(5) receptor subtype...
  33. ncbi request reprint Specifically immobilised aldo/keto reductase AKR1A1 shows a dramatic increase in activity relative to the randomly immobilised enzyme
    Kai Holland-Nell
    Institute of Biochemistry, University of Leipzig, Bruderstrasse 34, 04103 Leipzig
    Chembiochem 8:1071-6. 2007
    ..Furthermore, the enzyme was surprisingly stable. No loss of activity was observed for over a week, and even after 50 days more than 35% of activity was maintained...
  34. doi request reprint In vitro modification of substituted cysteines as tool to study receptor functionality and structure-activity relationships
    Daniel Rathmann
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, D 04103 Leipzig, Germany
    Anal Biochem 439:173-83. 2013
    ..This method pinpoints to residues being accessible but also presents potential molecular positions to investigate the global conformation. ..
  35. ncbi request reprint Proliferative effect of calcitonin gene-related peptide is induced by at least five proteins as identified by proteome profiling
    Franka Pluder
    Institute of Biochemistry, Faculty of Bioscience, Pharmacy and Psychology, University of Leipzig, Bruderstrasse 34, D 04103 Leipzig, Germany
    Chem Biol Drug Des 69:14-22. 2007
    ....
  36. doi request reprint An aromatic region to induce a switch between agonism and inverse agonism at the ghrelin receptor
    Sylvia Els
    Institute of Biochemistry, Universitat Leipzig, Brüderstraße 34, 04103 Leipzig, Germany
    J Med Chem 55:7437-49. 2012
    ..Hence, only minor changes in the peptide sequence can decide between agonism and inverse agonism and have a major impact on the biological activity...
  37. ncbi request reprint Fluorescence resonance energy transfer to study receptor dimerization in living cells
    Jurgen E Bader
    Institute of Biochemistry, University of Leipzig, Germany
    Methods Mol Biol 259:335-52. 2004
    ..A detailed description of a spectroscopic method for FRET studies in the field of GPCR interaction is presented to facilitate and propagate studies to increase our understanding of protein-protein interactions involving GPCRs...
  38. doi request reprint Adiponectin receptor 1 interacts with both subunits of protein kinase CK2
    Cathleen Juhl
    Institute of Biochemistry, Pharmacy and Psychology, Leipzig University, Leipzig, Germany
    Mol Cell Biochem 356:185-9. 2011
    ..Consequently, our data suggest an interaction of the adiponectin receptor 1 with the tetrameric complex and identified protein kinase CK2 as a key player in adiponectin signaling...
  39. doi request reprint Pyridone dipeptide backbone scan to elucidate structural properties of a flexible peptide segment
    Michael Haack
    Institute of Biochemistry, Universitat Leipzig, Bruderstr 34, D 04103 Leipzig, Germany
    J Am Chem Soc 130:8326-36. 2008
    ..The amphiphilic C-terminal helix of 3-fold-substituted NPY thus becomes visible by selective line broadening in the (1)H NMR. Accordingly, we could structurally characterize protein segments that are too flexible for other methods...
  40. doi request reprint Molecular recognition of the NPY hormone family by their receptors
    Diana Lindner
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy, and Psychology, Leipzig University, Leipzig, Germany
    Nutrition 24:907-17. 2008
    ..The data on the variation of the ligands and an overview of the currently known mutagenesis data are summarized and specific models for the binding mode of the three ligands in all four receptors are provided...
  41. doi request reprint Prokaryotic expression, in vitro folding, and molecular pharmacological characterization of the neuropeptide Y receptor type 2
    Peter Schmidt
    Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstrasse 16 18, D 04107 Leipzig, Germany
    Biotechnol Prog 25:1732-9. 2009
    ..In competition experiments, an IC(50) value in low nanomolar range could be determined. Further, a K(D) value of 1.9 nM was determined from a saturation assay, where NPY was titrated to the recombinant Y(2) receptors...
  42. ncbi request reprint Structural investigations of a human calcitonin-derived carrier peptide in a membrane environment by solid-state NMR
    Kerstin Wagner
    Institute of Biochemistry, University of Leipzig, Germany
    Biochemistry 43:12459-68. 2004
    ..These structures are maintained over a wide range of peptide:lipid mixing ratios. No direct indications for fibril formation of hCT(9-32) were found. Dipolar coupling measurements indicate rather high amplitudes of motion of the peptide...
  43. doi request reprint Investigation of lysine side chain interactions of interleukin-8 with heparin and other glycosaminoglycans studied by a methylation-NMR approach
    Kristin Möbius
    Department of Medicine, Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstraße 16 18, D 04107 Leipzig, Germany
    Glycobiology 23:1260-9. 2013
    ..We identified K25, K69 and K72 to be the most relevant binding anchors of IL-8(1-77) for the analyzed GAGs. ..
  44. ncbi request reprint Identification of neuropeptide Y cleavage products in human blood to improve metabolic stability
    Irfan Ullah Khan
    Institute of Biochemistry, University of Leipzig, Bruederstr 34, 04103 Leipzig, Germany
    Biopolymers 88:182-9. 2007
    ..Furthermore, the N-terminally CF-labeled Y1 and Y2 receptor-selective peptides were found to be enzymatically more resistant than their counterparts containing the CF label at Lys4 side chain...
  45. doi request reprint Functional role of the extracellular N-terminal domain of neuropeptide Y subfamily receptors in membrane integration and agonist-stimulated internalization
    Diana Lindner
    Institute of Biochemistry, Leipzig University, Bruderstrasse 34, 04103 Leipzig, Germany
    Cell Signal 21:61-8. 2009
    ....
  46. doi request reprint The anterograde transport of the human neuropeptide Y2 receptor is regulated by a subtype specific mechanism mediated by the C-terminus
    Cornelia Walther
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Universitat Leipzig, Germany
    Neuropeptides 46:335-43. 2012
    ..Whereas the 8th helix is involved before or at the level of Sar1 dependent export pathways in the ER for the hY(2)R, in hY(4)R helix eight is involved at later stages of anterograde transport...
  47. doi request reprint On-resin synthesis of an acylated and fluorescence-labeled cyclic integrin ligand for modification of poly(lactic-co-glycolic acid)
    Rayk Hassert
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Universitat Leipzig, Bruderstrasse 34, D 04103 Leipzig, Germany
    Chem Biodivers 9:2648-58. 2012
    ..This will provide the opportunity to introduce a variety of effector molecules site-specifically to the c[RGDfK] lipopeptide, which will enable the introduction of multifunctionality into c[RGDfK]-coated PLGA devices or nanoparticles...
  48. doi request reprint Identification of a potential modification site in human stromal cell-derived factor-1
    Lars Baumann
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Bruderstrasse 34, D 04103 Leipzig, Germany
    Biopolymers 94:771-8. 2010
    ..The study shows that lysine 56 is a potential site to introduce labels site-specifically in SDF-1...
  49. doi request reprint Selective labelling of stromal cell-derived factor 1α with carboxyfluorescein to study receptor internalisation
    Kathrin Bellmann-Sickert
    Institute of Biochemistry, Leipzig University, Leipzig, Germany
    J Pept Sci 16:568-74. 2010
    ..These results show that site-specific modification of SDF1α yields in a biologically functional molecule that allows the characterisation of CXCR4 production of cells on a molecular level...
  50. doi request reprint Access to gram scale amounts of functional globular adiponectin from E. coli inclusion bodies by alkaline-shock solubilization
    John T Heiker
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Bruderstrasse 34, 04103 Leipzig, Germany
    Biochem Biophys Res Commun 398:32-7. 2010
    ....
  51. ncbi request reprint Calcitonin-derived peptide carriers: mechanisms and application
    Robert Rennert
    Institute of Biochemistry, Faculty of Bioscience, Pharmacy and Psychology, Leipzig University, Bruderstr 34, D 04103 Leipzig, Germany
    Adv Drug Deliv Rev 60:485-98. 2008
    ..The current state of the art is reviewed concerning the structural requirements, mechanistic assumptions and metabolic features of these peptides as well as experiments proving their excellent carrier potential...
  52. ncbi request reprint Isotope-labeled cross-linkers and Fourier transform ion cyclotron resonance mass spectrometry for structural analysis of a protein/peptide complex
    Christian Ihling
    Biotechnological Biomedical Center, Faculty of Chemistry and Mineralogy, University of Leipzig, Leipzig, Germany
    J Am Soc Mass Spectrom 17:1100-13. 2006
    ....
  53. ncbi request reprint Expressed protein ligation. Method and applications
    Ralf David
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Germany
    Eur J Biochem 271:663-77. 2004
    ..Recently, alternative ligation approaches have been developed that do not require Cys residues. Accordingly, it is theoretically possible to obtain each modified protein using ligation strategies...
  54. ncbi request reprint Facile and selective nanoscale labeling of peptides in solution by using photolabile protecting groups
    Norman Koglin
    Institute of Biochemistry, University of Leipzig, Bruderstrasse 34, D 04103 Leipzig, Germany
    J Med Chem 46:4369-72. 2003
    ..Therefore selective labeling may become feasible for the first time even in nanomol amounts...
  55. ncbi request reprint Membrane interaction of neuropeptide Y detected by EPR and NMR spectroscopy
    Lars Thomas
    University of Leipzig, Institute of Medical Physics and Biophysics, Hartelstr 16 18, D 04107 Leipzig, Germany
    Biochim Biophys Acta 1714:103-13. 2005
    ..An estimation of the transfer energy of the peptide from aqueous solution to the membrane interface DeltaG supports the preferential interaction of NPY with negatively charged membranes...
  56. ncbi request reprint Analogues of neuropeptide Y containing beta-aminocyclopropane carboxylic acids are the shortest linear peptides that are selective for the Y1 receptor
    Norman Koglin
    Institut fur Biochemie, Universitat Leipzig, Talstrasse 33, 04103 Leipzig, Germany
    Angew Chem Int Ed Engl 42:202-5. 2003
  57. doi request reprint Proteolytic activation of prochemerin by kallikrein 7 breaks an ionic linkage and results in C-terminal rearrangement
    Stephan Schultz
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Universitat Leipzig, Bruderstrasse 34, D 04103 Leipzig, Germany
    Biochem J 452:271-80. 2013
    ..Therefore this interaction represents an interesting target for psoriasis therapy and treatment of other obesity-related diseases...
  58. doi request reprint A novel, biased-like SDF-1 derivative acts synergistically with starPEG-based heparin hydrogels and improves eEPC migration in vitro
    Lars Baumann
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Universitat Leipzig, Germany
    J Control Release 162:68-75. 2012
    ..We found higher migration rates compared to conventional SDF-1α. In summary, we provide a conceptual work on cooperative effects of enzymatically activatable SDF-1α and starPEG-heparin hydrogels...
  59. doi request reprint Incorporation of ortho-carbaboranyl-Nε-modified L-lysine into neuropeptide Y receptor Y1- and Y2-selective analogues
    Verena M Ahrens
    Universität Leipzig, Institute of Biochemistry, Brüderstraße 34, 04103 Leipzig
    J Med Chem 54:2368-77. 2011
    ..Internalization studies revealed excellent and receptor subtype specific uptake of the conjugates into respective cells...
  60. ncbi request reprint Comparative analysis of the signaling capabilities of the insulin receptor-related receptor
    Jürgen Klammt
    Hospital for Children and Adolescents, University of Leipzig, 04317 Leipzig, Oststrasse 21 25, Germany
    Biochem Biophys Res Commun 327:557-64. 2005
    ..The reestablishment of autophosphorylation was paralleled by restoration of interaction with a specific range of signaling molecules...
  61. doi request reprint Peptides and peptide conjugates: therapeutics on the upward path
    Verena M Ahrens
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Universitat Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    Future Med Chem 4:1567-86. 2012
    ..Stabilization strategies and intelligent linkers are discussed with respect to their use in peptide drug therapy...
  62. doi request reprint Ghrelin receptor: high constitutive activity and methods for developing inverse agonists
    Sylvia Els
    Institute of Biochemistry, Leipzig University, Brüderstrasse, Leipzig, Germany
    Methods Enzymol 485:103-21. 2010
    ..Peptide synthesis was performed on solid phase using a Fmoc/tBu-strategy. Peptide potency was measured with a signal transduction assay, the inositol trisphosphate turnover assay, adapted to a receptor expressing constitutive activity...
  63. ncbi request reprint Structure-activity studies of orexin a and orexin B at the human orexin 1 and orexin 2 receptors led to orexin 2 receptor selective and orexin 1 receptor preferring ligands
    Manja Lang
    Institute of Biochemistry, University of Leipzig, Germany
    J Med Chem 47:1153-60. 2004
    ..In addition, we identified orexin A 2-33 as the first analogue with orexin 1 receptor preference and orexin B 10-28, [A27]orexin B 6-28, and [P11]orexin B 6-28 as being highly potent orexin 2 receptor selective (>1000-fold) peptides...
  64. ncbi request reprint Electron paramagnetic resonance backbone dynamics studies on spin-labelled neuropeptide Y analogues
    Andrea Bettio
    Institute of Biochemistry, University of Leipzig, Talstrasse 33, D 04103 Leipzig, Germany
    J Pept Sci 8:671-82. 2002
    ..Furthermore, we demonstrated that TOAC-labelllng is a valuable tool to investigate changes in the backbone conformation and dynamics. This may be of major importance for peptides and small proteins when they bind to cell membranes...
  65. doi request reprint Peptide drugs to target G protein-coupled receptors
    Kathrin Bellmann-Sickert
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Bruderstrasse 34, D 04103 Germany
    Trends Pharmacol Sci 31:434-41. 2010
    ..This review focuses on peptides targeting G protein-coupled receptors that are promising drug candidates or that have recently entered the pharmaceutical market...
  66. ncbi request reprint Homodimerization of neuropeptide y receptors investigated by fluorescence resonance energy transfer in living cells
    Michaela C Dinger
    Institute of Biochemistry, University of Leipzig, Germany
    J Biol Chem 278:10562-71. 2003
    ..However, homodimerization is influenced neither by NPY nor by Galpha protein binding...
  67. ncbi request reprint Expressed enzymatic ligation for the semisynthesis of chemically modified proteins
    Zuzana Machova
    Institut für Biochemie der Universität Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    Angew Chem Int Ed Engl 42:4916-8. 2003
  68. ncbi request reprint Semisynthesis and characterization of the first analogues of pro-neuropeptide y
    Regula von Eggelkraut-Gottanka
    Institute of Biochemistry University of Leipzig Talstrasse 33, 04103 Leipzig, Germany
    Chembiochem 4:425-33. 2003
    ..Western blot analysis revealed that all five proNPY analogues are recognized by monoclonal antibodies directed against NPY as well as against the Cflanking peptide of NPY (CPON)...
  69. ncbi request reprint Tracking of human Y receptors in living cells--a fluorescence approach
    Ilka Böhme
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Bruderstr 34, D 04103 Leipzig, Germany
    Peptides 28:226-34. 2007
    ..Here we compare the application of the different fluorescent tags EYFP, Lumiotrade mark and SNAPtrade mark to track hY(1) and hY(5) receptors in living cells...
  70. ncbi request reprint Developing novel hCT derived cell-penetrating peptides with improved metabolic stability
    Robert Rennert
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Bruderstr 34, D 04103 Leipzig, Germany
    Biochim Biophys Acta 1758:347-54. 2006
    ..Since our metabolically more stable compounds are still able to pass the cell membrane they provide powerful tools as drug delivery vectors...
  71. pmc The neuropeptide Y monomer in solution is not folded in the pancreatic-polypeptide fold
    Andrea Bettio
    Institute of Biochemistry, University of Leipzig, Talstrasse 33, D 04103 Leipzig, Germany
    Protein Sci 11:1834-44. 2002
    ..Our results complete the structural characterization of NPY in solution at concentration ranges in which NMR experiments are not feasible. Furthermore, these results open the way to study the conformation of the receptor-bound ligand...
  72. ncbi request reprint Toward the application of proteomics to human thyroid tissue
    Kerstin Krause
    III Medical Department, University of Leipzig, Leipzig, Germany
    Thyroid 16:1131-43. 2006
    ..In this paper we describe for the first time a systematic approach to proteome analysis of human thyroid tissue...
  73. doi request reprint Structure-activity studies of RFamide peptides reveal subtype-selective activation of neuropeptide FF1 and FF2 receptors
    Maria Findeisen
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Brüderstraße 34, 04103 Leipzig, Germany
    ChemMedChem 6:1081-93. 2011
    ..These data provide new insight into the subtype-specific agonistic activation of the NPFF₁ and NPFF(2) receptors that are necessary for the development of selective agonists...
  74. ncbi request reprint Proteome analysis to study signal transduction of G protein-coupled receptors
    Franka Pluder
    Institute of Biochemistry, University of Leipzig, Bruderstrasse 34, D 04103, Leipzig, Germany
    Pharmacol Ther 112:1-11. 2006
    ..Here, we will summarize the current state of the art of proteome analysis applied to GPCR...
  75. ncbi request reprint Novel daunorubicin-carrier peptide conjugates derived from human calcitonin segments
    Ulrike Krauss
    Institute of Biochemistry, University of Leipzig, Bruderstr 34, 04103 Leipzig, Germany
    J Mol Recognit 16:280-7. 2003
    ....
  76. ncbi request reprint Expressed protein ligation for protein semisynthesis and engineering
    Zuzana Machova
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy, and Psychology, University of Leipzig, Germany
    Methods Mol Biol 298:105-30. 2005
    ....
  77. doi request reprint Protein kinase CK2 interacts with adiponectin receptor 1 and participates in adiponectin signaling
    John T Heiker
    Institute of Biochemistry, Faculty of Biociences, Pharmacy and Psychology, Leipzig University, Bruderstrasse 34, Leipzig, Germany
    Cell Signal 21:936-42. 2009
    ..g. leptin and insulin, our findings suggest a possible key function in crosstalk between adiponectin and insulin signaling pathways and could provide further insight into the anti-diabetic effects of adiponectin...
  78. ncbi request reprint Targeted tumor diagnosis and therapy with peptide hormones as radiopharmaceuticals
    Irfan Ullah Khan
    Institute of Biochemistry, Leipzig University, Bruderstr 34, 04103 Leipzig, Germany
    Anticancer Agents Med Chem 8:186-99. 2008
    ..Here, we summarize the variety of experiences gained in the development of various peptide analogs, chelator/radiolabeling techniques for applications in tumor imaging and therapy...
  79. ncbi request reprint Expressed protein ligation to obtain selectively modified aldo/keto reductases
    Michael P O Richter
    Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Bruderstrasse 34, D 04103 Leipzig, Germany
    Protein Pept Lett 12:777-81. 2005
    ..Applying this technique to enzymes of the aldo/keto reductase superfamily provides a new approach to generate native or modified redox enzymes for direct and indirect immobilization...
  80. ncbi request reprint Cluster properties of peptides on (100) semiconductor surfaces
    Karsten Goede
    Universitat Leipzig, Institut für Experimentelle Physik II, Linnestrasse 5, 04103 Leipzig, Germany
    Langmuir 22:8104-8. 2006
    ..Average cluster diameters vary from 40 nm on GaAs (100) to 300 nm on Si (100). Direct adhesion of the peptides to the surface competes with forming molecular aggregates that offer an overall reduced surface contact...
  81. ncbi request reprint In vitro gene delivery by a novel human calcitonin (hCT)-derived carrier peptide
    Ulrike Krauss
    Institute of Biochemistry, University of Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
    Bioorg Med Chem Lett 14:51-4. 2004
    ..Because of the peptide's physiological origin, cytotoxic effects are not expected...
  82. ncbi request reprint Ala31-Aib32: identification of the key motif for high affinity and selectivity of neuropeptide Y at the Y5-receptor
    Chiara Cabrele
    Institute of Biochemistry, University of Leipzig, Talstrasse 33, 04103 Leipzig, Germany
    Biochemistry 41:8043-9. 2002
    ..These results suggest that the selectivity of the Ala(31)-Aib(32) motif for the Y(5)-receptor derives from a specific conformation that must be correlated with the bioactive conformation of NPY at this subtype...
  83. ncbi request reprint Novel cell line selectively expressing neuropeptide Y-Y2 receptors
    Martin M Höfliger
    Institute of Biochemistry, University of Leipzig, Leipzig, Germany
    J Recept Signal Transduct Res 23:351-60. 2003
    ..In both cell lines binding of NPY induced signal transduction, which was monitored as reduction of forskolin-induced cAMP production in an ELISA...
  84. doi request reprint Visfatin/PBEF/Nampt: structure, regulation and potential function of a novel adipokine
    Grit Sommer
    Department of Internal Medicine III, University of Leipzig, 04103 Leipzig, Germany
    Clin Sci (Lond) 115:13-23. 2008
    ..12.). Moreover, reports on the structure and hormonal regulation of visfatin/PBEF/Nampt have given further insights into its potential physiological role. The present review summarizes studies on visfatin/PBEF/Nampt as a novel adipokine...
  85. ncbi request reprint Strongly altered receptor binding properties in PP and NPY chimeras are accompanied by changes in structure and membrane binding
    Mirjam Lerch
    Institute of Pharmaceutical Sciences, ETH Zurich, Switzerland
    Biochemistry 44:9255-64. 2005
    ..Differences in receptor binding properties between the chimeras and their parental peptides are therefore most likely due to changes in the conformation of the micelle-bound peptides...
  86. ncbi request reprint Determination of affinity and activity of ligands at the human neuropeptide Y Y4 receptor by flow cytometry and aequorin luminescence
    Ralf Ziemek
    Institut fur Pharmazie, Universitat Regensburg, Regensburg, Germany
    J Recept Signal Transduct Res 27:217-33. 2007
    ..17), a lead to be optimized in terms of potency and selectivity...
  87. ncbi request reprint The SK-N-MC cell line expresses an orexin binding site different from recombinant orexin 1-type receptor
    Heike A Wieland
    Division of Preclinical Research, Boehringer Ingelheim Pharma KG, Biberach, Germany
    Eur J Biochem 269:1128-35. 2002
    ..Amino acid prediction of this A -->G change results in an isoleucine --> valine substitution at the protein level, which may provide evidence for an editing process...
  88. doi request reprint Promiscuous modification of the nuclear poly(A)-binding protein by multiple protein-arginine methyltransferases does not affect the aggregation behavior
    Katharina Fronz
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    J Biol Chem 283:20408-20. 2008
    ..Thus, arginine methylation might be relevant for oculopharyngeal muscular dystrophy. However, in two different types of assays we have been unable to detect any effect of arginine methylation on the aggregation of bovine PABPN1...
  89. ncbi request reprint Multiple NPY receptors Inhibit GABA(A) synaptic responses of rat medial parvocellular effector neurons in the hypothalamic paraventricular nucleus
    Nina Pronchuk
    Department of Pharmacology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Endocrinology 143:535-43. 2002
    ..Controversy regarding the receptor or receptor subtypes involved in NPY-mediated feeding may arise from the multiple NPY receptors present...
  90. ncbi request reprint Fluorescence- and luminescence-based methods for the determination of affinity and activity of neuropeptide Y2 receptor ligands
    Ralf Ziemek
    University of Regensburg, Institute of Pharmacy, Universitätsstr 31, D 93040 Regensburg, Germany
    Eur J Pharmacol 551:10-8. 2006
    ..Functional data of Y(2) receptor agonists and antagonists determined in the fluorescence- and luminescence-based assays were in good agreement...
  91. ncbi request reprint GPR39 signaling is stimulated by zinc ions but not by obestatin
    Birgitte Holst
    Laboratory for Molecular Pharmacology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK 2200 Copenhagen, Denmark
    Endocrinology 148:13-20. 2007
    ..In contrast, the potency and efficacy of Zn(2+) in respect of activating signaling indicates that this metal ion could be a physiologically relevant agonist or modulator of GPR39...
  92. ncbi request reprint Decoding the entry of two novel cell-penetrating peptides in HeLa cells: lipid raft-mediated endocytosis and endosomal escape
    Christina Foerg
    Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology Zurich ETH Zurich, Switzerland
    Biochemistry 44:72-81. 2005
    ..The results bear potential for CPP as tools for intracellular drug delivery...
  93. pmc Metabolic cleavage of cell-penetrating peptides in contact with epithelial models: human calcitonin (hCT)-derived peptides, Tat(47-57) and penetratin(43-58)
    Rachel Tréhin
    Drug Formulation and Delivery Group, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology Zurich ETH Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Biochem J 382:945-56. 2004
    ..The results, particularly on hCT(9-32), may be used as a template to suggest structural modifications towards improved CPP performance...
  94. ncbi request reprint Bilayer interaction and localization of cell penetrating peptides with model membranes: a comparative study of a human calcitonin (hCT)-derived peptide with pVEC and pAntp(43-58)
    Michael E Herbig
    Drug Formulation and Delivery Group, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology Zurich ETH Zurich, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
    Biochim Biophys Acta 1712:197-211. 2005
    ..Generally, we found strong peptide-phospholipid interactions, especially when negatively charged lipids were present...
  95. pmc Membrane surface-associated helices promote lipid interactions and cellular uptake of human calcitonin-derived cell penetrating peptides
    Michael E Herbig
    Drug Formulation and Delivery Group, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology Zurich ETH Zurich, CH 8093 Zurich, Switzerland
    Biophys J 89:4056-66. 2005
    ..Liposome leakage studies excluded pore formation, and the punctuated fluorescence pattern of internalized peptide indicated vesicular localization and, in conclusion, strongly suggested an endocytic pathway of translocation...