Affiliation: University of Greifswald
Schmölders J, Manske C, Otto A, Hoffmann C, Steiner B, Welin A, et al
. Comparative Proteomics of Purified Pathogen Vacuoles Correlates Intracellular Replication of Legionella pneumophila with the Small GTPase Ras-related protein 1 (Rap1). Mol Cell Proteomics. 2017;16:622-641 pubmed publisher
..pneumophila Thus, comparative proteomics identified Rap1 as a novel LCV host component implicated in intracellular replication of L. pneumophila. ..
Maaß S, Moog G, Becher D. Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry. Methods Mol Biol. 2019;1921:445-464 pubmed publisher
..pneumophila to different environmental and nutritional situations during infection or during different life cycle stages including planktonic or biofilm phase. ..
Maaß S, Otto A, Albrecht D, Riedel K, Trautwein Schult A, Becher D. Proteomic Signatures of Clostridium difficile Stressed with Metronidazole, Vancomycin, or Fidaxomicin. Cells. 2018;7: pubmed publisher
..Notably, the data provided by this study hint also at so far unknown antibiotic detoxification mechanisms. ..
Handtke S, Volland S, Methling K, Albrecht D, Becher D, Nehls J, et al
. Cell physiology of the biotechnological relevant bacterium Bacillus pumilus-an omics-based approach. J Biotechnol. 2014;192 Pt A:204-14 pubmed publisher
..In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed. ..
Bonn F, Pane Farre J, Schlüter R, Schaffer M, Fuchs S, Bernhardt J, et al
. Global analysis of the impact of linezolid onto virulence factor production in S. aureus USA300. Int J Med Microbiol. 2016;306:131-40 pubmed publisher
..A new hypothesis is discussed, which suggests that the reduced amount of membrane and extracellular proteins and observed defects in cell division are due to the disintegration of transertion complexes by linezolid. ..
Bonn F, Maass S, Becher D. Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research. Methods Mol Biol. 2017;1520:281-289 pubmed publisher
..In addition, a focus has been set to the specific challenges in antibiotic stress research. ..
Hoyer J, Bartel J, Gómez Mejia A, Rohde M, Hirschfeld C, Heß N, et al
. Proteomic response of Streptococcus pneumoniae to iron limitation. Int J Med Microbiol. 2018;308:713-721 pubmed publisher
..Based on the results, it is shown that the pneumococcal proteome response to iron limitation is strongly dependent on the initial iron concentration in the medium or the environment. ..
Abendroth U, Adlung N, Otto A, GrÃ¼neisen B, Becher D, Bonas U. Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas euvesicatoria. BMC Genomics. 2017;18:625 pubmed publisher
..Additionally, our proteogenomic analyses validates "hypothetical" proteins and will improve annotation of Xanthomonadaceae genomes, providing a solid basis for further studies. ..
Otto A, Maas S, Bonn F, Büttner K, Becher D. An Easy and Fast Protocol for Affinity Bead-Based Protein Enrichment and Storage of Proteome Samples. Methods Enzymol. 2017;585:1-13 pubmed publisher
..Hence, we evaluated the reduction of time for sample preparation by use of preprimed affinity beads and shorter incubation durations as well as the influence of high concentration of salts or urea in the sample buffer. ..