Ralf Bartenschlager

Summary

Affiliation: University of Heidelberg
Country: Germany

Publications

  1. ncbi request reprint Replication of the hepatitis C virus in cell culture
    Ralf Bartenschlager
    Department for Molecular Virology, University of Heidelberg, Otto Meyerhof Zentrum, Im Neuenheimer Feld 350, 69120 Heidelberg, Germany Ralf_
    Antiviral Res 60:91-102. 2003
  2. pmc Structural and functional characterization of nonstructural protein 2 for its role in hepatitis C virus assembly
    Vlastimil Jirasko
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Biol Chem 283:28546-62. 2008
  3. pmc Analysis of hepatitis C virus superinfection exclusion by using novel fluorochrome gene-tagged viral genomes
    Torsten Schaller
    Department for Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 81:4591-603. 2007
  4. pmc Role of annexin A2 in the production of infectious hepatitis C virus particles
    Perdita Backes
    Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 84:5775-89. 2010
  5. pmc Efficient trans-encapsidation of hepatitis C virus RNAs into infectious virus-like particles
    Eike Steinmann
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 82:7034-46. 2008
  6. ncbi request reprint From structure to function: new insights into hepatitis C virus RNA replication
    Nicole Appel
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Biol Chem 281:9833-6. 2006
  7. pmc Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras
    Thomas Pietschmann
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 103:7408-13. 2006
  8. pmc Mutational analysis of hepatitis C virus nonstructural protein 5A: potential role of differential phosphorylation in RNA replication and identification of a genetically flexible domain
    Nicole Appel
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 79:3187-94. 2005
  9. pmc Cell culture adaptation of hepatitis C virus and in vivo viability of an adapted variant
    Artur Kaul
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 81:13168-79. 2007
  10. ncbi request reprint The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient virus assembly
    Anna Shavinskaya
    Department of Molecular Virology, University of Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 282:37158-69. 2007

Detail Information

Publications68

  1. ncbi request reprint Replication of the hepatitis C virus in cell culture
    Ralf Bartenschlager
    Department for Molecular Virology, University of Heidelberg, Otto Meyerhof Zentrum, Im Neuenheimer Feld 350, 69120 Heidelberg, Germany Ralf_
    Antiviral Res 60:91-102. 2003
    ..Ultimately, the replicon system may help to decipher the molecular basis of interferon-alpha (IFN-alpha) resistance...
  2. pmc Structural and functional characterization of nonstructural protein 2 for its role in hepatitis C virus assembly
    Vlastimil Jirasko
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Biol Chem 283:28546-62. 2008
    ..Finally, we demonstrate that mutations in NS2 blocking HCV assembly can be rescued by trans-complementation...
  3. pmc Analysis of hepatitis C virus superinfection exclusion by using novel fluorochrome gene-tagged viral genomes
    Torsten Schaller
    Department for Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 81:4591-603. 2007
    ..By using these genomes, we found that HCV induces superinfection exclusion, which is primarily due to interference at a post-entry step...
  4. pmc Role of annexin A2 in the production of infectious hepatitis C virus particles
    Perdita Backes
    Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 84:5775-89. 2010
    ..These data identify ANXA2 as a novel host factor contributing, with NS5A, to the formation of infectious HCV particles...
  5. pmc Efficient trans-encapsidation of hepatitis C virus RNAs into infectious virus-like particles
    Eike Steinmann
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 82:7034-46. 2008
    ..Similar to other vector systems of plus-strand RNA viruses, HCV(TCP) may prove valuable for gene delivery or vaccination approaches...
  6. ncbi request reprint From structure to function: new insights into hepatitis C virus RNA replication
    Nicole Appel
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Biol Chem 281:9833-6. 2006
  7. pmc Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras
    Thomas Pietschmann
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 103:7408-13. 2006
    ..Finally, we describe an E1-specific antiserum capable of neutralizing infectivity of all HCV chimeras...
  8. pmc Mutational analysis of hepatitis C virus nonstructural protein 5A: potential role of differential phosphorylation in RNA replication and identification of a genetically flexible domain
    Nicole Appel
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 79:3187-94. 2005
    ..This region was found to tolerate even large insertions with only a moderate effect on replication. Based on these results, we propose a model of the role of NS5A phosphorylation in the viral life cycle...
  9. pmc Cell culture adaptation of hepatitis C virus and in vivo viability of an adapted variant
    Artur Kaul
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 81:13168-79. 2007
    ..These results argue for some level of impaired fitness of this mutant in vivo...
  10. ncbi request reprint The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient virus assembly
    Anna Shavinskaya
    Department of Molecular Virology, University of Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 282:37158-69. 2007
    ..Taken together, our results demonstrate that the binding strength of the D2 domain of core for LDs is crucial for determining the efficiency of virus assembly...
  11. pmc Efficient hepatitis C virus cell culture system: what a difference the host cell makes
    Ralf Bartenschlager
    Dept of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 102:9739-40. 2005
  12. pmc Identification of determinants involved in initiation of hepatitis C virus RNA synthesis by using intergenotypic replicase chimeras
    Marco Binder
    University of Heidelberg, Department of Molecular Virology, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 81:5270-83. 2007
    ....
  13. ncbi request reprint Novel insights into hepatitis C virus replication and persistence
    Ralf Bartenschlager
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    Adv Virus Res 63:71-180. 2004
    ..This chapter attempts to summarize the current state of knowledge in these most actively worked on fields of HCV research...
  14. ncbi request reprint The non-structural protein 4A of dengue virus is an integral membrane protein inducing membrane alterations in a 2K-regulated manner
    Sven Miller
    Department of Molecular Virology, University of Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 282:8873-82. 2007
    ..These results shed new light on the role of NS4A in the DV replication cycle and provide a model of how this protein induces membrane rearrangements and how this property may be regulated...
  15. pmc Efficient rescue of hepatitis C virus RNA replication by trans-complementation with nonstructural protein 5A
    Nicole Appel
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 79:896-909. 2005
    ..In summary, our results provide the first insight into the functional organization of the HCV replication complex...
  16. pmc Role of RNA structures in genome terminal sequences of the hepatitis C virus for replication and assembly
    Peter Friebe
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 83:11989-95. 2009
    ..In addition, we show that the 5' nontranslated region of the genome most likely does not contain cis-acting RNA structures required for RNA packaging into infectious virions...
  17. pmc Viral and cellular determinants of hepatitis C virus RNA replication in cell culture
    Volker Lohmann
    Department of Molecular Virology, Otto Meyerhof Zentrum, Im Neuenheimer Feld 350, 69120 Heidelberg, Germany
    J Virol 77:3007-19. 2003
    ..In summary, these data show that the efficiency of HCV replication in cell culture is determined both by adaptation of the viral sequence and by the host cell itself...
  18. pmc Biochemical and morphological properties of hepatitis C virus particles and determination of their lipidome
    Andreas Merz
    Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Biol Chem 286:3018-32. 2011
    ..Taken together, we describe a rapid and efficient system for the production of large quantities of affinity-purified HCV allowing a comprehensive analysis of the infectious virion, including the determination of its lipid composition...
  19. ncbi request reprint The hepatitis C virus replicon system: from basic research to clinical application
    Ralf Bartenschlager
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Hepatol 43:210-6. 2005
  20. doi request reprint Molecular aspects of Dengue virus replication
    Ralf Bartenschlager
    Department of Molecular Virology, Im Neuenheimer Feld 345, University of Heidelberg, D 69120 Heidelberg, Germany
    Future Microbiol 3:155-65. 2008
    ..In this review we will highlight recent insights into the molecular mechanisms of the viral replication cycle...
  21. pmc A comprehensive structure-function comparison of hepatitis C virus strain JFH1 and J6 polymerases reveals a key residue stimulating replication in cell culture across genotypes
    Melanie Schmitt
    Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, D 69120 Heidelberg, Germany
    J Virol 85:2565-81. 2011
    ..Finally, we were able to enhance the replication efficiency of a genotype 1b isolate with the I405 mutation, indicating that this mechanism of action is conserved across genotypes...
  22. ncbi request reprint Hepatitis C virus molecular clones: from cDNA to infectious virus particles in cell culture
    Ralf Bartenschlager
    Department of Molecular Virology, Hygiene Institut, University Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    Curr Opin Microbiol 9:416-22. 2006
    ..Without doubt, this system provides new possibilities for molecular studies of the HCV life cycle and the development of novel antiviral concepts...
  23. ncbi request reprint Hepatitis C virus RNA replication is resistant to tumour necrosis factor-alpha
    Michael Frese
    Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Freiburg, Hermann Herder Str 11, D 79104 Freiburg, Germany
    J Gen Virol 84:1253-9. 2003
    ..It is, therefore, unlikely that the increased production of TNF-alpha, which is seen in many hepatitis C patients, contributes to HCV clearance by inducing antiviral defence mechanisms in infected hepatocytes...
  24. ncbi request reprint Hepatitis C virus molecular clones and their replication capacity in vivo and in cell culture
    Ralf Bartenschlager
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    Virus Res 127:195-207. 2007
    ..In this review, we will summarize the development and current state of molecular HCV clones and discuss the prospects and implications of the most recent achievements...
  25. doi request reprint Reconstructing signaling pathways from RNAi data using probabilistic Boolean threshold networks
    Lars Kaderali
    Viroquant Research Group Modeling, University of Heidelberg, Bioquant BQ26, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany
    Bioinformatics 25:2229-35. 2009
    ..Difficulties are aggravated if not all pathway components can be observed after each knockdown, but readouts are only available for a small subset. We are then facing the problem of reconstructing a network from incomplete data...
  26. ncbi request reprint Hepatitis C virus replicons: potential role for drug development
    Ralf Bartenschlager
    Department of Molecular Virology, Institute of Hygiene, University of Heidelberg, Otto Meyerhof Zentrum, Im Neuenheimer Feld 350, 69120 Heidelberg, Germany
    Nat Rev Drug Discov 1:911-6. 2002
    ..However, the recent development of a replicon system opens up an encouraging possibility for drug discovery...
  27. pmc Characterization of the early steps of hepatitis C virus infection by using luciferase reporter viruses
    George Koutsoudakis
    Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, Heidelberg 69120, Germany
    J Virol 80:5308-20. 2006
    ..The reporter viruses described here should be instrumental for investigating the viral life cycle and for the development of HCV inhibitors...
  28. pmc Ribavirin antagonizes the in vitro anti-hepatitis C virus activity of 2'-C-methylcytidine, the active component of valopicitabine
    Lotte Coelmont
    Rega Institute for Medical Research, KULeuven, Leuven, Belgium
    Antimicrob Agents Chemother 50:3444-6. 2006
    ..These findings may have implications when planning clinical studies with valopicitabine...
  29. pmc The level of CD81 cell surface expression is a key determinant for productive entry of hepatitis C virus into host cells
    George Koutsoudakis
    Department Molecular Virology, University Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 81:588-98. 2007
    ..Together these data indicate that a high density of cell surface-exposed CD81 is a key determinant for productive HCV entry into host cells...
  30. pmc The hepatitis C virus RNA 3'-untranslated region strongly enhances translation directed by the internal ribosome entry site
    Yutong Song
    Institute of Biochemistry, Faculty of Medicine, Justus Liebig University Giessen, Friedrichstrasse 24, 35392 Giessen, Germany
    J Virol 80:11579-88. 2006
    ....
  31. pmc euHCVdb: the European hepatitis C virus database
    Christophe Combet
    Institut de Biologie et Chimie des Protéines UMR5086, CNRS, University of Lyon 1, IFR 128 BioSciences, Lyon Gerland, France
    Nucleic Acids Res 35:D363-6. 2007
    ..As part of an international collaborative effort with the US and Japanese databases, the European HCV Database (euHCVdb) is mainly dedicated to HCV protein sequences, 3D structures and functional analyses...
  32. ncbi request reprint Antiviral effects of amantadine and iminosugar derivatives against hepatitis C virus
    Eike Steinmann
    Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
    Hepatology 46:330-8. 2007
    ..Furthermore, HCV was rapidly eliminated from cell culture upon passage in the presence of a long alkyl chain deoxynojirimycin (DNJ). CONCLUSION: Iminosugar derivatives are potential drugs for treatment of HCV infections...
  33. ncbi request reprint Hepatitis C virus escape from the interferon regulatory factor 3 pathway by a passive and active evasion strategy
    Marco Binder
    Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
    Hepatology 46:1365-74. 2007
    ..Conclusion: Based on these findings, we propose a refined model of innate immune escape by HCV involving limited initial induction and stringent subsequent control of the IRF-3 response...
  34. ncbi request reprint Enhancement of genotype 1 hepatitis C virus replication by bile acids through FXR
    Caroline Scholtes
    INSERM U851, IFR 128 Biosciences Lyon Gerland, Lyon F 69007, France
    J Hepatol 48:192-9. 2008
    ..Besides, BAs are essential factors for replication of the porcine enteric calicivirus by inhibiting interferon signaling. The role of BAs on HCV RNA replication was thus assessed...
  35. pmc Essential role of domain III of nonstructural protein 5A for hepatitis C virus infectious particle assembly
    Nicole Appel
    Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
    PLoS Pathog 4:e1000035. 2008
    ..Since domain III of NS5A is one of the most variable regions in the HCV genome, the results suggest that viral isolates may differ in their level of virion production and thus in their level of fitness and pathogenesis...
  36. ncbi request reprint Interferon-gamma inhibits replication of subgenomic and genomic hepatitis C virus RNAs
    Michael Frese
    Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Freiburg, Hermann Herder Strasse 11, D 79104 Freiburg, Germany
    Hepatology 35:694-703. 2002
    ....
  37. pmc Comparative in vitro anti-hepatitis C virus activities of a selected series of polymerase, protease, and helicase inhibitors
    Jan Paeshuyse
    Rega Institute for Medical Research, KU Leuven, Leuven, Belgium
    Antimicrob Agents Chemother 52:3433-7. 2008
    ....
  38. pmc Hepatitis C virus p7 protein is crucial for assembly and release of infectious virions
    Eike Steinmann
    Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
    PLoS Pathog 3:e103. 2007
    ....
  39. ncbi request reprint Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b
    Kyoko Murakami
    Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Virology 351:381-92. 2006
    ..We conclude that the replicon-based 3D culture system allows the production of infectious HCV particles. This system is a valuable tool in studies of HCV morphogenesis in a natural host cell environment...
  40. ncbi request reprint High density lipoprotein inhibits hepatitis C virus-neutralizing antibodies by stimulating cell entry via activation of the scavenger receptor BI
    Marlene Dreux
    INSERM, U758, 69007 Lyon, France
    J Biol Chem 281:18285-95. 2006
    ..Consistently, we show that antibodies targeted to HCV-E1 efficiently neutralize HCVpp and HCVcc in the presence of human serum...
  41. ncbi request reprint Interferon type I gene expression in chronic hepatitis C
    Sabine Mihm
    Division of Gastroenterology and Endocrinology, Department of Internal Medicine, Georg August Universitat, Gottingen, Germany
    Lab Invest 84:1148-59. 2004
    ..In conclusion, our data provide evidence for the absence of an induction of type I IFN genes by HCV in the human liver and argue for a further development of type I IFN-based therapies...
  42. ncbi request reprint Application of the trak-C HCV core assay for monitoring antiviral activity in HCV replication systems
    Laurence Cagnon
    Johnson and Johnson Pharmaceutical Research and Development, L L C, 3210 Merryfield Row, San Diego, CA 92121, USA
    J Virol Methods 118:23-31. 2004
    ..Given its ease of use and the lack of requirement for RNA purification, the trak-C assay has several advantages over RNA-based methods of viral monitoring...
  43. pmc Alternative approaches for efficient inhibition of hepatitis C virus RNA replication by small interfering RNAs
    Jan Krönke
    Department of Molecular Virology, Hygiene Institute, University of Heidelberg, D 69120 Heidelberg, Germany
    J Virol 78:3436-46. 2004
    ..These results demonstrate that the retroviral transduction of HCV-specific shRNAs provides a new possibility for antiviral intervention...
  44. ncbi request reprint A replicon-based bioassay for the measurement of interferons in patients with chronic hepatitis C
    Jan M Vrolijk
    Department of Hepatology and Gastroenterology, Erasmus University Medical Center, Rotterdam, The Netherlands
    J Virol Methods 110:201-9. 2003
    ..8 to 4.1 ng/ml in patients treated with PEG IFN-alpha. In conclusion, the HCV-replicon based bioassay allows determining the levels of biologically active IFN-alpha in serum and heparinized plasma of patients under treatment...
  45. pmc Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons
    Rainer Gosert
    Department of Medicine II, University of Freiburg, Germany
    J Virol 77:5487-92. 2003
    ..Metabolic labeling with 5-bromouridine 5'-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV...
  46. ncbi request reprint Identification and characterization of amphiphysin II as a novel cellular interaction partner of the hepatitis C virus NS5A protein
    Birgit Zech
    Axxima Pharmaceuticals AG, Am Klopferspitz 19, 82152 Martinsried, Germany
    J Gen Virol 84:555-60. 2003
    ....
  47. pmc The hepatitis C virus RNA-dependent RNA polymerase membrane insertion sequence is a transmembrane segment
    Natalia Ivashkina
    Department of Medicine II, University Hospital, University of Freiburg, Hugstetter Strasse 55, D 79106 Freiburg, Germany
    J Virol 76:13088-93. 2002
    ..These findings have important implications for the functional architecture of the HCV replication complex...
  48. pmc Mutations that permit efficient replication of hepatitis C virus RNA in Huh-7 cells prevent productive replication in chimpanzees
    Jens Bukh
    Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:14416-21. 2002
    ..Our study demonstrates that mutations that are adaptive for replication of HCV in cell culture may be highly attenuating in vivo...
  49. ncbi request reprint Serum versus intrahepatic HCV RNA and liver histology
    Volker Dries
    Hepatology 35:1552-3. 2002
  50. pmc Genetic analysis of sequences in the 3' nontranslated region of hepatitis C virus that are important for RNA replication
    Peter Friebe
    Institute for Virology, Johannes Gutenberg University Mainz, 55131 Mainz, Germany
    J Virol 76:5326-38. 2002
    ..These data represent the first detailed mapping of sequences in the 3' NTR assumed to act as a promoter for initiation of minus-strand RNA synthesis...
  51. pmc Persistent and transient replication of full-length hepatitis C virus genomes in cell culture
    Thomas Pietschmann
    Institute for Virology, Johannes Gutenberg University Mainz, 55131 Mainz, Germany
    J Virol 76:4008-21. 2002
    ..These results suggest that Huh-7 cells lack host cell factors that are important for virus particle assembly and/or release...
  52. pmc Membrane association of the RNA-dependent RNA polymerase is essential for hepatitis C virus RNA replication
    Darius Moradpour
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 44, CH 1011 Lausanne, Switzerland
    J Virol 78:13278-84. 2004
    ..These results have implications for the functional architecture of the HCV replication complex and provide new insights into the expanding spectrum of tail-anchored proteins...
  53. pmc Kissing-loop interaction in the 3' end of the hepatitis C virus genome essential for RNA replication
    Peter Friebe
    Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
    J Virol 79:380-92. 2005
    ..These data provide strong evidence for a pseudoknot structure at the 3' end of the HCV genome that is essential for replication...
  54. ncbi request reprint The non-immunosuppressive cyclosporin DEBIO-025 is a potent inhibitor of hepatitis C virus replication in vitro
    Jan Paeshuyse
    Rega Institute for Medical Research, Leuven, Belgium
    Hepatology 43:761-70. 2006
    ....
  55. ncbi request reprint Subcellular localization and membrane topology of the Dengue virus type 2 Non-structural protein 4B
    Sven Miller
    Department of Molecular Virology, The University of Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 281:8854-63. 2006
    ..This topology model provides a good starting point for a detailed investigation of the function of NS4B in the DV life cycle...
  56. ncbi request reprint The face of future hepatitis C antiviral drug development: recent biological and virologic advances and their translation to drug development and clinical practice
    John G McHutchison
    Division of Gastroenterology, Duke Clinical Research Institute, Duke University Medical Centre, 2400 Pratt Street, Room 0311, Terrace Level, Durham, NC 27707, USA
    J Hepatol 44:411-21. 2006
  57. ncbi request reprint E2 of hepatitis C virus inhibits apoptosis
    Song Hee Lee
    Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Kyungbuk, Korea
    J Immunol 175:8226-35. 2005
    ..Based on these results, we propose that E2 augments persistent HCV infection by blocking host-induced apoptosis of infected cells...
  58. pmc Dissecting the interferon-induced inhibition of hepatitis C virus replication by using a novel host cell line
    Marc P Windisch
    Department of Molecular Virology, Hygiene Institute, University of Heidelberg, Im Neuenheimer Feld 345, D 69120 Heidelberg, Germany
    J Virol 79:13778-93. 2005
    ..Taken together, the results establish HuH6 replicon cells as a valuable tool for IFN studies and for the evaluation of antiviral compounds...
  59. pmc Quantitative analysis of the hepatitis C virus replication complex
    Doris Quinkert
    Department Molecular Virology, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    J Virol 79:13594-605. 2005
    ....
  60. ncbi request reprint Hepatitis C virus-replicating hepatocytes induce fibrogenic activation of hepatic stellate cells
    Anja Schulze-Krebs
    Department of Medicine I, University of Erlangen Nuremberg, Germany
    Gastroenterology 129:246-58. 2005
    ....
  61. ncbi request reprint Hepatitis C virus NS2/3 processing is required for NS3 stability and viral RNA replication
    Sarah Welbourn
    McGill Cancer Center and Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    J Biol Chem 280:29604-11. 2005
    ..The regulation of NS2/3 cleavage could constitute a novel mechanism of switching between viral RNA replication and other processes of the hepatitis C virus life cycle...
  62. pmc Production of infectious hepatitis C virus in tissue culture from a cloned viral genome
    Takaji Wakita
    Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo 183 8526, Japan
    Nat Med 11:791-6. 2005
    ..The cell culture-generated HCV is infectious for chimpanzee. This system provides a powerful tool for studying the viral life cycle and developing antiviral strategies...
  63. ncbi request reprint In vitro models for hepatitis C
    Ralf Bartenschlager
    University of Mainz, Johannes Gutenberg University of Mainz, Institute for Virology, Germany
    Virus Res 82:25-32. 2002
  64. ncbi request reprint Tissue culture and animal models for hepatitis C virus
    Thomas Pietschmann
    Department of Molecular Virology, University of Heidelberg, 350 Otto Meyerhof Zentrum Im Neuenheimer Feld, Heidelberg 69120, Germany
    Clin Liver Dis 7:23-43. 2003
    ..These developments would at last allow us to model the complete viral life cycle, something researchers have been struggling with ever since the first identification of HCV...
  65. ncbi request reprint Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nature 437:1167-72. 2005
    ..Cardif thus functions as an adaptor, linking the cytoplasmic dsRNA receptor RIG-I to the initiation of antiviral programmes...
  66. pmc Sustained delivery of siRNAs targeting viral infection by cell-degradable multilayered polyelectrolyte films
    Maria Dimitrova
    Institut National de la Sante et de la Recherche Medicale, U748, 3 Rue Koeberle, F 67000 Strasbourg, France
    Proc Natl Acad Sci U S A 105:16320-5. 2008
    ..Moreover, this MPF-based delivery system may represent a promising previously undescribed perspective for the use of RNAi as a therapeutic strategy for human diseases...
  67. ncbi request reprint Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81
    Mirjam B Zeisel
    Institut National de la Sante et de la Recherche Medicale INSERM, U748, Strasbourg, France
    Hepatology 46:1722-31. 2007
    ..Conclusion: Our data suggest that SR-BI (i) represents a key host factor for HCV entry, (ii) is implicated in the same HCV entry pathway as CD81, and (iii) targets an entry step closely linked to HCV-CD81 interaction...
  68. ncbi request reprint HCV proteins increase expression of heme oxygenase-1 (HO-1) and decrease expression of Bach1 in human hepatoma cells
    Tahereh Ghaziani
    Department of Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 1111, USA
    J Hepatol 45:5-12. 2006
    ..Other recent work has implicated Bach1, a heme binding protein that represses gene expression, in the regulation of HO-1 gene expression...