Research Topics
| Kenichi AtakaSummaryAffiliation: University of Bielefeld Country: Germany Publications
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Detail Information
Publications
Biochemical applications of surface-enhanced infrared absorption spectroscopyKenichi Ataka
Department of Chemistry, Biophysical Chemistry PC III, Bielefeld University, 33615, Bielefeld, Germany
Anal Bioanal Chem 388:47-54. 2007..Thus, surface-modification techniques are addressed that enable control of the proper orientation of proteins on the metal surface...
Molecular impact of the membrane potential on the regulatory mechanism of proton transfer in sensory rhodopsin IIXiue Jiang
Department of Chemistry, Biophysical Chemistry PC III, Bielefeld University, 33615 Bielefeld, Germany
J Am Chem Soc 132:10808-15. 2010....
Photosynthetic hydrogen production by a hybrid complex of photosystem I and [NiFe]-hydrogenaseHenning Krassen
Department of Chemistry, Bielefeld University, D 33615 Bielefeld, Germany
ACS Nano 3:4055-61. 2009..The successful demonstration of efficient solar-to-hydrogen conversion may serve as a blueprint for the establishment of this system in a living organism with the paramount advantage of self-replication...
Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: design of a catalytic surface for the production of molecular hydrogenHenning Krassen
Bielefeld University, Department of Chemistry, D 33615 Bielefeld, Germany
J Biotechnol 142:3-9. 2009..In conclusion, a viable hydrogen-evolving surface was developed that may be employed in combination with immobilized photosystems to provide a platform for hydrogen production from water and solar energy with enzymes as catalysts...
In situ monitoring of the orientated assembly of strep-tagged membrane proteins on the gold surface by surface enhanced infrared absorption spectroscopyXiue Jiang
Bielefeld University, Department of Chemistry, Biophysical Chemistry, 33615 Bielefeld, Germany
Phys Chem Chem Phys 10:6381-7. 2008..The specificity of strep-tagged ecgltP to the streptavidin layer is with 60% only modest. Nevertheless, the streptavidin/biotin interlayer reveals a higher regeneration propensity than the His-tag/Ni-NTA interlayer...
Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer levelKenichi Ataka
Biophysical Chemistry, Department of Chemistry, Bielefeld University, 33615 Bielefeld, Germany
Biochem Soc Trans 36:986-91. 2008..Functionality of such biomimetic systems is assessed in situ by spectro-electrochemical methods...
Use of surface enhanced infrared absorption spectroscopy (SEIRA) to probe the functionality of a protein monolayerKenichi Ataka
, IBI-2: Structural Biology, , Germany
Biopolymers 82:415-9. 2006..The presented methodology opens new avenues to study the mechanism of the biomedically relevant class of electron and voltage-gated proteins on the atomic level...
Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrodeKenichi Ataka
, IBI-2: Structural Biology, , Germany
J Phys Chem B 110:9339-47. 2006..The approach is considered to be of general applicability as the introduction of affinity tags is routine in current biochemistry...
Scanning probe microscopic studies of the oriented attachment and membrane reconstitution of cytochrome C oxidase to a gold electrodeDirk Mayer
Research Center Juelich, ISG-2: Institute for Bio and Chemosensors, IBI-2: Structural Biology, D-52425 Juelich, Germany
Langmuir 21:8580-3. 2005..Finally, the reconstitution of the proteins within the supported membrane was verified. The amount of oxidase immobilized within the solid supported membrane was estimated...
Oriented attachment and membrane reconstitution of His-tagged cytochrome c oxidase to a gold electrode: in situ monitoring by surface-enhanced infrared absorption spectroscopyKenichi Ataka
, IBI-2: Structural Biology, , Germany
J Am Chem Soc 126:16199-206. 2004..Finally, full functionality of the surface-tethered cytochrome c oxidase was demonstrated by cyclic voltammetry after binding of the natural electron donor cytochrome c...
Functional vibrational spectroscopy of a cytochrome c monolayer: SEIDAS probes the interaction with different surface-modified electrodesKenichi Ataka
, IBI-2: Structural Biology, , Germany
J Am Chem Soc 126:9445-57. 2004..Finally, we critically discuss our results within the framework of the different models for cytochrome c adsorption on CMEs...
The molecular mechanism of membrane proteins probed by evanescent infrared wavesRebecca M Nyquist
, IBI-2: Structural Biology, , Germany
Chembiochem 5:431-6. 2004..Functional changes as minute as the protonation state of individual amino acid side chains can be observed and linked to interactions with a ligand, agonist, effector, or redox partner...
Electrochemically induced surface-enhanced infrared difference absorption (SEIDA) spectroscopy of a protein monolayerKenichi Ataka
, IBI-2: Structural Biology, , Germany
J Am Chem Soc 125:4986-7. 2003..The observed differences in the relative band intensities as compared to the difference spectrum of cytochrome c in solution are attributed to orientational effects of the adsorbed protein...
