Josef Altenbuchner

Summary

Affiliation: University of Stuttgart
Country: Germany

Publications

  1. pmc Characterization of a mannose utilization system in Bacillus subtilis
    Tianqi Sun
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    J Bacteriol 192:2128-39. 2010
  2. pmc Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors
    Kambiz Morabbi Heravi
    Institut für Industrielle Genetik, Universitat Stuttgart, Germany
    Microb Cell Fact 10:83. 2011
  3. doi request reprint Construction of a dual-tag system for gene expression, protein affinity purification and fusion protein processing
    Hassan Motejadded
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Biotechnol Lett 31:543-9. 2009
  4. pmc Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation
    Marian Wenzel
    Institut für Industrielle Genetik, Universitat Stuttgart, Stuttgart, Germany
    Appl Environ Microbiol 77:6419-25. 2011
  5. doi request reprint Biosynthesis of zeaxanthin in recombinant Pseudomonas putida
    Holger Beuttler
    Institute of Technical Biochemistry, Universitaet Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Appl Microbiol Biotechnol 89:1137-47. 2011
  6. doi request reprint The Escherichia coli rhamnose promoter rhaP(BAD) is in Pseudomonas putida KT2440 independent of Crp-cAMP activation
    Marcel Jeske
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569, Stuttgart, Germany
    Appl Microbiol Biotechnol 85:1923-33. 2010
  7. doi request reprint Characterization of the tyrosine recombinase MrpA encoded by the Streptomyces coelicolor A3(2) plasmid SCP2*
    Lydia Warth
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Arch Microbiol 193:187-200. 2011
  8. pmc Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
    Angelika Wegerer
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    BMC Biotechnol 8:2. 2008
  9. pmc Development of a method for markerless gene deletion in Pseudomonas putida
    Nadja Graf
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Appl Environ Microbiol 77:5549-52. 2011
  10. pmc Degradation of alkyl methyl ketones by Pseudomonas veronii MEK700
    Christina Onaca
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    J Bacteriol 189:3759-67. 2007

Collaborators

  • Vlada B Urlacher
  • Andreas Stolz
  • Angelika Wegerer
  • Marian Wenzel
  • Marcel Jeske
  • Hassan Motejadded
  • Iris Haug
  • Lydia Warth
  • Nadja Graf
  • Kambiz Morabbi Heravi
  • Holger Beuttler
  • Tianqi Sun
  • Sven Rustler
  • Christina Onaca
  • Tina Stumpp
  • Rolf D Schmid
  • Bernhard Hauer
  • Alexander Muller
  • Martin Siemann-Herzberg
  • Jana Hoffmann
  • Martin Kieninger
  • Karl H Engesser
  • Sarah Himbert
  • Stephen Bentley
  • Tobias Kieser
  • Dirk Brolle
  • Anke Weissenborn

Detail Information

Publications13

  1. pmc Characterization of a mannose utilization system in Bacillus subtilis
    Tianqi Sun
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    J Bacteriol 192:2128-39. 2010
    ..subtilis CCR system. Only in the double mutant with an HPr Ser46Ala mutation and a crh knockout mutation was CCR strongly reduced. In contrast, P(manR) and P(manP) were not inducible in a ccpA deletion mutant...
  2. pmc Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors
    Kambiz Morabbi Heravi
    Institut für Industrielle Genetik, Universitat Stuttgart, Germany
    Microb Cell Fact 10:83. 2011
    ..In this study, we investigated the regulation of the mtl genes in order to identify the elements needed to construct a strong mannitol inducible expression system in B. subtilis...
  3. doi request reprint Construction of a dual-tag system for gene expression, protein affinity purification and fusion protein processing
    Hassan Motejadded
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Biotechnol Lett 31:543-9. 2009
    ..After proteolytic cleavage the products were applied to a Ni-NTA column to remove protease and tags. Pure eGFP protein was obtained in the flow-through of the column in a yield of around 35% of the crude cell extract...
  4. pmc Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation
    Marian Wenzel
    Institut für Industrielle Genetik, Universitat Stuttgart, Stuttgart, Germany
    Appl Environ Microbiol 77:6419-25. 2011
    ..6%. The novel B. subtilis self-induction system thus makes a considerable contribution to improving product yield and reducing the costs associated with its technical application...
  5. doi request reprint Biosynthesis of zeaxanthin in recombinant Pseudomonas putida
    Holger Beuttler
    Institute of Technical Biochemistry, Universitaet Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Appl Microbiol Biotechnol 89:1137-47. 2011
    ..Particularly, the addition of lecithin during cell cultivation increased volumetric productivity of Pseudomonas putida by a factor of 4.7 (51 mg/l vs. 239 mg/l)...
  6. doi request reprint The Escherichia coli rhamnose promoter rhaP(BAD) is in Pseudomonas putida KT2440 independent of Crp-cAMP activation
    Marcel Jeske
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569, Stuttgart, Germany
    Appl Microbiol Biotechnol 85:1923-33. 2010
    ..Other global regulators like Crc, PtsN, and CyoB had no or minor effects on rhamnose-induced eGFP expression. Therefore, this expression system may also be generally useful for Pseudomonas and other gamma-proteobacteria...
  7. doi request reprint Characterization of the tyrosine recombinase MrpA encoded by the Streptomyces coelicolor A3(2) plasmid SCP2*
    Lydia Warth
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Arch Microbiol 193:187-200. 2011
    ..The results define MrpA as a new site-specific tyrosine recombinase that acts with mrpS. In addition, we suggest that Y354 provides the nucleophile for DNA cleavage during recombination...
  8. pmc Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
    Angelika Wegerer
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    BMC Biotechnol 8:2. 2008
    ..A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules...
  9. pmc Development of a method for markerless gene deletion in Pseudomonas putida
    Nadja Graf
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Appl Environ Microbiol 77:5549-52. 2011
    ..The genes were efficiently disrupted and left a markerless chromosomal in-frame deletion...
  10. pmc Degradation of alkyl methyl ketones by Pseudomonas veronii MEK700
    Christina Onaca
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    J Bacteriol 189:3759-67. 2007
    ..coli and purified by immobilized metal affinity chromatography. The protein exhibited high esterase activity towards short chain esters like ethyl acetate and 4-nitrophenyl acetate...
  11. ncbi request reprint Cloning of the netropsin resistance genes from Streptomyces flavopersicus NRRL 2820
    Tina Stumpp
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    J Basic Microbiol 45:355-62. 2005
    ..Deletion analysis showed that both proteins are necessary for netropsin resistance indicating that the proteins form a heterodimeric ABC-transporter exporting netropsin...
  12. doi request reprint Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid
    Sven Rustler
    Institut fur Mikrobiologie, Universitat Stuttgart, Allmandring 31, Stuttgart, Germany
    Appl Microbiol Biotechnol 80:87-97. 2008
    ..The chiral analysis of the products formed revealed a high enantiomeric excess for the (S)-enantiomers...
  13. ncbi request reprint Streptomyces coelicolor A3(2) plasmid SCP2*: deductions from the complete sequence
    Iris Haug
    Institut für Industrielle Genetik, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Microbiology 149:505-13. 2003
    ..IS1648 belongs to the IS3 family of insertion sequences. The second element, Tn5417, shows the highest similarity to the Tn4811 element located in the terminal inverted repeats of the Streptomyces lividans chromosome...