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Genomes and Genes | Herbert TomasoSummaryCountry: Germany Publications
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Publications
Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella sppSascha Al Dahouk
Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
Clin Chem Lab Med 45:1464-70. 2007..We therefore assessed the performance of real-time PCR assays for the identification of members of the genus Brucella to the genus and species level...- Rapid detection of Yersinia pestis with multiplex real-time PCR assays using fluorescent hybridisation probesHerbert Tomaso
Military Hospital Innsbruck, Ministry of Defence, Innsbruck, Austria
FEMS Immunol Med Microbiol 38:117-26. 2003..We developed and evaluated a highly specific real-time PCR strategy for the detection of Y. pestis, obtaining results within 3 h including DNA preparation... 
Preliminary validation of real-time PCR assays for the identification of Yersinia pestisHerbert Tomaso
Bundeswehr Institute of Microbiology, Munich, Germany
Clin Chem Lab Med 46:1239-44. 2008..Therefore, rapid and reliable diagnostic tools are crucial. The objective of this study was to assess the inter-laboratory reproducibility of in-house developed real-time PCR assays for the identification of Y. pestis...- Real-time PCR using hybridization probes for the rapid and specific identification of Francisella tularensis subspecies tularensisHerbert Tomaso
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
Mol Cell Probes 21:12-6. 2007..This is a major diagnostic improvement, as all other methods for the specific identification of F. tularensis subsp. tularensis are more time consuming... - Development of a 5'-nuclease real-time PCR assay targeting fliP for the rapid identification of Burkholderia mallei in clinical samplesHerbert Tomaso
Bundeswehr Institute of Microbiology, Munich, Germany
Clin Chem 52:307-10. 2006..The number of reported outbreaks has increased steadily during the last decade, but diagnosis is hampered by the low bacterial load in infected tissues and excretions... 
Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCRHerbert Tomaso
Friedrich Loeffler Institut, Institute of Bacterial Infections and Zoonoses, Naumburgerstrasse 96a, 07743 Jena, Germany
BMC Infect Dis 10:100. 2010..Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA...- [Mission oriented diagnostic real-time PCR]Herbert Tomaso
Militärspital Innsbruck, Innsbruck, Osterreich
Wien Klin Wochenschr 119:26-32. 2007..In a bioterrorist scenario samples have to be treated according to rules applied in forensic medicine and documentation has to be flawless... - Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probesHerbert Tomaso
Federal Armed Forces Institute of Microbiology, Neuherbergstrasse 11, Munich 80937, Germany
Mol Cell Probes 19:9-20. 2005..In conclusion, we recommend the use of the 16S rDNA and/or fliC real-time PCR assays for the rapid identification of B. mallei and B. pseudomallei in positive blood cultures or from suspicious bacterial colonies... - Brucella inopinata sp. nov., isolated from a breast implant infectionHolger C Scholz
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
Int J Syst Evol Microbiol 60:801-8. 2010..Because of its unexpected isolation, the name Brucella inopinata with the type strain BO1(T) (=BCCN 09-01(T)=CPAM 6436(T)) is proposed... - Brucella microti sp. nov., isolated from the common vole Microtus arvalisHolger C Scholz
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
Int J Syst Evol Microbiol 58:375-82. 2008..nov. with the type strain CCM 4915(T) (=BCCN 07-01(T)=CAPM 6434(T)) is proposed... - Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA geneHolger Christian Scholz
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
J Med Microbiol 57:64-71. 2008..The developed assay closes a diagnostic gap and provides a simple but robust tool for the sensitive detection and correct identification of O. anthropi, O. intermedium and Brucella spp... - Genetic diversity and phylogenetic relationships of bacteria belonging to the Ochrobactrum-Brucella group by recA and 16S rRNA gene-based comparative sequence analysisHolger C Scholz
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
Syst Appl Microbiol 31:1-16. 2008..In summary, Ochrobactrum is a highly diverse genus comprising several novel species. We recommend recA- in addition to rrs gene-analysis for correct species allocation and subtyping of novel Ochrobactrum isolates... - Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower AustriaHolger Christian Scholz
Bundeswehr Institute of Microbiology, Munich, Germany
Vector Borne Zoonotic Dis 9:153-6. 2009..Our findings demonstrate that B. microti is prevalent in a larger geographic area covering the region of South Moravia and parts of Lower Austria. Foxes could have become infected by ingestion of infected common voles... - Detection of the reemerging agent Burkholderia mallei in a recent outbreak of glanders in the United Arab Emirates by a newly developed fliP-based polymerase chain reaction assayHolger C Scholz
Bundeswehr Institute of Microbiology, D 80937 Munich, Germany
Diagn Microbiol Infect Dis 54:241-7. 2006..mallei infection. The developed PCR assay can be used as a simple and rapid tool for the specific and sensitive detection of B. mallei in clinical samples... - Genotyping of Ochrobactrum anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysisHolger C Scholz
Bundeswehr Institute of Microbiology, Munich, Germany
FEMS Microbiol Lett 257:7-16. 2006..In summary, recA-sequence analysis provides a new reliable molecular subtyping tool to study the phylogeny of the Ochrobactrum taxon at both the inter- and intraspecies level... - Cross-border molecular tracing of brucellosis in EuropeMayada Gwida
Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institute, Jena, Germany
Comp Immunol Microbiol Infect Dis 35:181-5. 2012..In summary, cross-border molecular tracing confirmed brucellosis being a zoonosis of concern for European public health... - Identification of brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)Sascha Al Dahouk
Department of Bacteriology, Bundeswehr Institute of Microbiology, D 80937 Munich, Germany
Crit Rev Microbiol 31:191-6. 2005..Consequently, PCR-RFLP assays of specific gene loci can serve as tools for diagnostic, epidemiological, taxonomic, and evolutionary studies. Various PCR-RFLPs used for the identification of Brucella species and biotypes are reviewed... - Evaluation of Brucella MLVA typing for human brucellosisSascha Al Dahouk
Bundeswehr Institute of Microbiology, Department of Bacteriology, Neuherbergstr 11, D 80937 Munich, Germany
J Microbiol Methods 69:137-45. 2007..MLVA can significantly contribute to epidemiological trace-back analysis of Brucella infections and may advance surveillance and control of human brucellosis... - Detection of Yersinia pestis using real-time PCR in patients with suspected bubonic plagueJulia M Riehm
Bundeswehr Institute of Microbiology, Munich, Germany
Mol Cell Probes 25:8-12. 2011..In conclusion, the 5'-nuclease assay targeting pla can be recommended as diagnostic tool for establishing a presumptive diagnosis when bubonic plague is clinically suspected... - Identification and antimicrobial susceptibilities of Ochrobactrum sppBryan Thoma
Bundeswehr Institute of Microbiology, Munich, Germany
Int J Med Microbiol 299:209-20. 2009..Our in vitro data suggest that combinations of antimicrobial agents including ciprofloxacin and/or trimethoprim/sulfamethoxazole may be useful for empirical treatment of Ochrobactrum infections... - The detection of Brucella spp. using PCR-ELISA and real-time PCR assaysSascha Al Dahouk
Institute of Microbiology, Federal Armed Forces, Munich, Germany
Clin Lab 50:387-94. 2004..Advantages and disadvantages of the different methods for real-time detection of Brucella spp. and a newly developed PCR-ELISA system for the high throughput of clinical samples will be discussed... - Development of 5' nuclease real-time PCR assays for the rapid identification of the burkholderia mallei//burkholderia pseudomallei complexHerbert Tomaso
Institute of Microbiology, Federal Armed Forces, Munich, Germany
Diagn Mol Pathol 13:247-53. 2004..mallei/B. pseudomallei complex in positive blood cultures or from suspicious bacterial colonies allowing the early onset of appropriate antibiotic therapy... - Brucella endocarditis in prosthetic valvesSascha Al Dahouk
Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
Can J Cardiol 22:971-4. 2006..The patient was successfully treated by a medical and surgical approach. Therapeutic strategies in Brucella endocarditis are discussed in light of the current literature... - Detection of Chromobacterium violaceum by multiplex PCR targeting the prgI, spaO, invG, and sipB genesHolger Christian Scholz
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
Syst Appl Microbiol 29:45-8. 2006..The detection limit for pure cultures in multiplex PCR analysis was 100CFU. The developed assay significantly improves rapid identification of Chr. violaceum and allows its differentiation from closely related organisms... - Laboratory-based diagnosis of brucellosis--a review of the literature. Part II: serological tests for brucellosisSascha Al Dahouk
Institute of Microbiology, Federal Armed Forces, Munich, Germany
Clin Lab 49:577-89. 2003..This review will summarize advantages and disadvantages of the serological techniques used in clinical laboratories for indirect verification of human brucellosis... - Development of a diagnostic multiplex polymerase chain reaction microarray assay to detect and differentiate Brucella sppGernot Schmoock
Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, Jena, Germany
Diagn Microbiol Infect Dis 71:341-53. 2011..suis bv 3/4/B. canis and B. neotomae/B. microti. In summary, the newly developed Brucella ArrayTube® assay is an easy-to-handle molecular test for high-throughput and parallel analysis... - Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infectionsSascha Al Dahouk
Department of Bacteriology, Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
J Immunol Methods 309:34-47. 2006..abortus clearly from cross-reacting bacteria. The combination of SELDI-MS analysis with the specificity of antibody binding will improve the identification of Brucella specific immunogenic proteins... - Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysisHolger Christian Scholz
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
FEMS Microbiol Lett 244:347-52. 2005..In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence-based approach to specifically identify and classify C. violaceum on genospecies level... - Failure of a short-term antibiotic therapy for human brucellosis using ciprofloxacin. A study on in vitro susceptibility of Brucella strainsSascha Al Dahouk
Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
Chemotherapy 51:352-6. 2005..Human brucellosis is characterized byfocal complications, chronic courses, and therapeutic failures... - Comparison of diagnostic tests for the detection of Brucella spp. in camel seraMayada M Gwida
Friedrich Loeffler Institut, Institute for Bacterial Infections and Zoonoses, Jena, Germany
BMC Res Notes 4:525. 2011..abstract:.. - Laboratory-based diagnosis of brucellosis--a review of the literature. Part I: Techniques for direct detection and identification of Brucella sppSascha Al Dahouk
Institute of Microbiology, Federal Armed Forces, Munich, Germany
Clin Lab 49:487-505. 2003..This review will summarize advantages and disadvantages of the techniques used in clinical laboratories for direct detection and identification of Brucella spp... - Growth characteristics of Bacillus anthracis compared to other Bacillus spp. on the selective nutrient media Anthrax Blood Agar and Cereus Ident AgarHerbert Tomaso
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
Syst Appl Microbiol 29:24-8. 2006..Species which might be misidentified as B. anthracis were B. cereus, B. mycoides, and B. thuringiensis. Particularly, 30% of B. weihenstephanensis strains were misidentified as B. anthracis... - Antimicrobial susceptibilities of Austrian Francisella tularensis holarctica biovar II strainsHerbert Tomaso
Bundeswehr Institute of Microbiology, Munich, Germany
Int J Antimicrob Agents 26:279-84. 2005..Our in vitro susceptibility data can be applied for the detection and comparison of resistance development and to provide in vitro data for the guidance of therapy... - Clinical and virological characterization of imported cases of Chikungunya feverMartin Pfeffer
Bundeswehr Institute of Microbiology, Munich, Germany
Wien Klin Wochenschr 120:95-100. 2008..The spread of an African CHIKV to Asia further demonstrates how fast viruses can emerge and establish in places where competent vectors are prevalent... - Phenotypical Characterization of Mongolian Yersinia pestis StrainsDaniel Kiefer
Bundeswehr Institute of Microbiology, Munich, Germany
Vector Borne Zoonotic Dis 12:183-8. 2012..This strain was isolated from a wildlife rodent with no anthropogenic influence and thus suggests naturally acquired resistance... - Optimized application of surface-enhanced laser desorption/ionization time-of-flight MS to differentiate Francisella tularensis at the level of subspecies and individual strainsEric Seibold
Bundeswehr Institute of Microbiology, Neuherbergstr, Munchen, Germany
FEMS Immunol Med Microbiol 49:364-73. 2007..Furthermore, single strains within F. tularensis subspecies could be discriminated... - Brucellosis - regionally emerging zoonotic disease?Mayada Gwida
Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute for Bacterial Infections and Zoonoses, Naumburger Str 96a, 07743 Jena, Germany
Croat Med J 51:289-95. 2010..To gain deeper insight into the seroprevalence of brucellosis, which remains a zoonotic disease of worldwide public health concern, by reviewing studies from countries including North Africa, the Middle East, and India... - Rapid identification of Burkholderia mallei and Burkholderia pseudomallei by intact cell Matrix-assisted Laser Desorption/Ionisation mass spectrometric typingAxel Karger
Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Molecular Biology, Südufer 10, Greifswald Insel Riems D 17493, Germany
BMC Microbiol 12:229. 2012..The aim of this study was to evaluate the use of MALDI-TOF MS for the rapid and reliable identification and differentiation of B. pseudomallei and B. mallei and to build up a reliable reference database for both organisms... - Comparison of four commercially available assays for the detection of IgM phase II antibodies to Coxiella burnetii in the diagnosis of acute Q feverDimitrios Frangoulidis
Bundeswehr Institute of Microbiology, Neuherbergstr 11, 80937 Munich, Germany
Ann N Y Acad Sci 1078:561-2. 2006.... - Effects of hypoxia and reoxygenation on the expression levels of the urokinase-type plasminogen activator, its inhibitor plasminogen activator inhibitor type-1 and the urokinase-type plasminogen activator receptor in human head and neck tumour cellsLisa D Sprague
Institut für Molekulare Pathogenese, Friedrich Loeffler Institut, Bundesforschungsinstitut für Tiergesundheit, D 07743 Jena, Germany
Oncol Rep 17:1259-68. 2007.... - Yersinia pestis in Small Rodents, MongoliaJulia M Riehm
Bundeswehr Institute of Microbiology, Munich, Germany
Emerg Infect Dis 17:1320-2. 2011..However, human plague was first reported in 1897 (3), such infections have been documented since the 1940s, and Yersinia pestis can be found in many provinces of Mongolia (Figure; T. Damindorj, pers. comm.) (3,4)... - Determination of antimicrobial sensitivities of Campylobacter jejuni isolated from commercial turkey farms in GermanyHosny El-Adawy
Friedrich Loeffler Institut Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str 96a, 07743 Jena, Germany
Avian Dis 56:685-92. 2012..The phenotypic and genotypic resistance profiles were compatible in the case of ciprofloxacin and tetracycline but were not completely congruent with respect to erythromycin... - Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattleChristiane Schnee
Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Naumburger Strasse 96a, 07743 Jena, Germany
BMC Vet Res 7:47. 2011.... - Coexistence of pathogens in host-seeking and feeding ticks within a single natural habitat in Central GermanyJan Franke
Department of Food and Environmental Hygiene, Institute of Nutrition, Friedrich Schiller University Jena, Dornburger Str 29, 07743 Jena, Germany
Appl Environ Microbiol 76:6829-36. 2010..Our results suggest that birds not only are important reservoirs for several pathogens but also act as vehicles for infected ticks and might therefore play a key role in the dispersal of tick-borne diseases... - Prevalence of Coxiella burnetii in clinically healthy German sheep flocksAngela Hilbert
Institut fur Epidemiologie, Friedrich Loeffler Institut, National Reference Laboratory for Q fever, Wusterhausen, Germany
BMC Res Notes 5:152. 2012..burnetii (10% and 25%, respectively) was assessed at flock level in 39/252 randomly selected clinically healthy sheep flocks with more than 100 ewes and unknown abortion rate... - Isolation and characterization of Ochrobactrum anthropi and Ochrobactrum pecoris from caecal content of commercial turkeysHosny Eladawy
Friedrich Loeffler Institut Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str 96a, D 07743 Jena, Germany
Vet Microbiol 155:349-54. 2012..This is the first time that Ochrobactrum species were isolated from an avian host, i.e. turkey... - Genetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracisJaran S Olsen
Forsvarets forskningsinstitutt FFI, Norwegian Defence Research Establishment, P O Box 25, N 2027 Kjeller, Norway
J Microbiol Methods 71:265-74. 2007..anthracis strains analysed. This is one of the most thoroughly validated chromosomal B. anthracis markers for real-time PCR identification, in which the screened collection contained several very closely related B. anthracis strains... - Population structure of Francisella tularensisUlrich Nubel
Robert Koch Institute, Burgstr 37, 38855 Wernigerode, Germany
J Bacteriol 188:5319-24. 2006..novicida and F. tularensis subsp. mediasiatica were indicated to be phylogenetically incoherent taxa. Incongruent gene trees and mosaic structures of housekeeping genes provided evidence for genetic recombination in F. tularensis...