R Timpl

Summary

Country: Germany

Publications

  1. ncbi request reprint Fibulins: a versatile family of extracellular matrix proteins
    Rupert Timpl
    Laboratory of Protein Chemistry, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 4:479-89. 2003
  2. ncbi request reprint Structure and function of laminin LG modules
    R Timpl
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Matrix Biol 19:309-17. 2000
  3. ncbi request reprint Structural and functional analysis of the recombinant G domain of the laminin alpha4 chain and its proteolytic processing in tissues
    J F Talts
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Biol Chem 275:35192-9. 2000
  4. ncbi request reprint Short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin and proteins
    T Sasaki
    Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
    J Mol Biol 314:751-63. 2001
  5. ncbi request reprint Mapping of binding sites for nidogens, fibulin-2, fibronectin and heparin to different IG modules of perlecan
    M Hopf
    Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
    J Mol Biol 311:529-41. 2001
  6. ncbi request reprint The C-terminal domain V of perlecan promotes beta1 integrin-mediated cell adhesion, binds heparin, nidogen and fibulin-2 and can be modified by glycosaminoglycans
    J C Brown
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 250:39-46. 1997
  7. pmc Perinatal lethality and endothelial cell abnormalities in several vessel compartments of fibulin-1-deficient mice
    G Kostka
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Mol Cell Biol 21:7025-34. 2001
  8. pmc Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins
    J F Talts
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 18:863-70. 1999
  9. pmc Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin
    T Sasaki
    Max Planck Institut f r Biochemie, D 82152 Martinsried, Germany
    EMBO J 17:1606-13. 1998
  10. ncbi request reprint Tropoelastin binding to fibulins, nidogen-2 and other extracellular matrix proteins
    T Sasaki
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    FEBS Lett 460:280-4. 1999

Collaborators

Detail Information

Publications78

  1. ncbi request reprint Fibulins: a versatile family of extracellular matrix proteins
    Rupert Timpl
    Laboratory of Protein Chemistry, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 4:479-89. 2003
    ..New insights into their biological roles are now emerging from studies of transgenic mice and of some inherited human diseases...
  2. ncbi request reprint Structure and function of laminin LG modules
    R Timpl
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Matrix Biol 19:309-17. 2000
    ..Further modifications may be introduced by proteolytic processing of laminin G domains, which is known to occur in the alpha2, alpha3 and alpha4 chains...
  3. ncbi request reprint Structural and functional analysis of the recombinant G domain of the laminin alpha4 chain and its proteolytic processing in tissues
    J F Talts
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Biol Chem 275:35192-9. 2000
    ..Immunogold staining demonstrated an association of the alpha4 chain primarily with microfibrillar regions rather than with basement membranes, while laminin alpha2 chains appear primarily associated with various basement membranes...
  4. ncbi request reprint Short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin and proteins
    T Sasaki
    Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
    J Mol Biol 314:751-63. 2001
    ..Immunological studies showed a similar partial processing in cell culture and tissues and the persistence of the released fragment in tissues. This indicated that both N-terminal regions of the gamma2 chain may have a function in vivo...
  5. ncbi request reprint Mapping of binding sites for nidogens, fibulin-2, fibronectin and heparin to different IG modules of perlecan
    M Hopf
    Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
    J Mol Biol 311:529-41. 2001
    ..This implicates a complex cluster of heterotypic interaction sites apparently important for the supramolecular organization of perlecan in tissues...
  6. ncbi request reprint The C-terminal domain V of perlecan promotes beta1 integrin-mediated cell adhesion, binds heparin, nidogen and fibulin-2 and can be modified by glycosaminoglycans
    J C Brown
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 250:39-46. 1997
    ..05-0.2 microM and about 2 microM). Perlecan domain V therefore seems to participate in the supramolecular assembly and cell connections of basement membranes...
  7. pmc Perinatal lethality and endothelial cell abnormalities in several vessel compartments of fibulin-1-deficient mice
    G Kostka
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Mol Cell Biol 21:7025-34. 2001
    ..The data indicate a strong but restricted abnormality in some endothelial compartments which, together with some kidney and lung defects, may be responsible for early death...
  8. pmc Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins
    J F Talts
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 18:863-70. 1999
    ..This demonstrated similar binding repertoires for the LG modules of three basement membrane proteins involved in cell-matrix interactions and supramolecular assembly...
  9. pmc Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin
    T Sasaki
    Max Planck Institut f r Biochemie, D 82152 Martinsried, Germany
    EMBO J 17:1606-13. 1998
    ..Immunological assays demonstrated variable secretion by cultured human cells of M2BP, which was detected in the extracellular matrix of several mouse tissues...
  10. ncbi request reprint Tropoelastin binding to fibulins, nidogen-2 and other extracellular matrix proteins
    T Sasaki
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    FEBS Lett 460:280-4. 1999
    ..The colocalization of these proteins emphasizes the potential for in vivo interactions...
  11. ncbi request reprint Nidogen-2: a new basement membrane protein with diverse binding properties
    E Kohfeldt
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Mol Biol 282:99-109. 1998
    ..Both nidogens were cell-adhesive for a restricted number of cell lines, with nidogen-2 having a higher activity. Together, these data suggest that nidogen-2 can compensate for some but not all functional activities ascribed to nidogen-1...
  12. ncbi request reprint The N-terminal globular domain of the laminin alpha1 chain binds to alpha1beta1 and alpha2beta1 integrins and to the heparan sulfate-containing domains of perlecan
    N Ettner
    Max Planck Institut fur Biochemie, Martinsried, Germany
    FEBS Lett 430:217-21. 1998
    ..This localized the binding activities to the LN module, which contains two basic sequences suitable for heparin interactions...
  13. ncbi request reprint Binding of fibulin-1 to nidogen depends on its C-terminal globular domain and a specific array of calcium-binding epidermal growth factor-like (EG) modules
    S Adam
    Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
    J Mol Biol 272:226-36. 1997
    ..This indicates a complex epitope structure involving domains II and III, which each may provide binding epitopes or stabilize each other...
  14. ncbi request reprint Perlecan domain V of Drosophila melanogaster. Sequence, recombinant analysis and tissue expression
    M V Friedrich
    Max Planck Institut fur Biochemie, Germany
    Eur J Biochem 267:3149-59. 2000
    ..This indicates, as for mammalian species, a distinct role of perlecan during Drosophila development...
  15. ncbi request reprint Structural characterization of two variants of fibulin-1 that differ in nidogen affinity
    T Sasaki
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    J Mol Biol 245:241-50. 1995
    ..Self aggregation into nest-like oligomers was observed at high concentrations of fibulin-1 which was not sensitive to EDTA...
  16. ncbi request reprint Endostatins derived from collagens XV and XVIII differ in structural and binding properties, tissue distribution and anti-angiogenic activity
    T Sasaki
    Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
    J Mol Biol 301:1179-90. 2000
    ..Together, these data indentify endostatin-XV as an angiogenesis inhibitor, which differs from endostatin-XVIII in several important functional details...
  17. ncbi request reprint Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1
    M Hopf
    Abteilung Proteinchemie, Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Struct Biol 8:634-40. 2001
    ..A large surface patch on the beta-barrel is strikingly conserved in all metazoan nidogens. Site-directed mutagenesis demonstrates that the conserved residues are involved in perlecan binding...
  18. ncbi request reprint Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies
    A Ries
    Max Planck Institut fur Biochemie, Am Klopferspitz 18 A, D 82152 Martinsried, Germany
    Eur J Biochem 268:5119-28. 2001
    ..Three of the antibodies showed strongly reduced binding to G2 and G3 after complex formation with either a perlecan domain or laminin-1...
  19. pmc Dimer model for the microfibrillar protein fibulin-2 and identification of the connecting disulfide bridge
    T Sasaki
    Max Planck Institut fur Biochemie, Martinsried, Germany
    EMBO J 16:3035-43. 1997
    ..This was demonstrated by surface plasmon resonance assay which showed a distinct interaction between domains N and II with a Kd of approximately 0.7 microM...
  20. pmc A single EGF-like motif of laminin is responsible for high affinity nidogen binding
    U Mayer
    Max Planck Institut fur Biochemie, Martinsried, Germany
    EMBO J 12:1879-85. 1993
    ..This identifies repeat 4 as the only binding structure. The sequence of repeat 4 is well conserved in the human and in part in the Drosophila laminin B2 chain.(ABSTRACT TRUNCATED AT 250 WORDS)..
  21. ncbi request reprint Different susceptibilities of fibulin-1 and fibulin-2 to cleavage by matrix metalloproteinases and other tissue proteases
    T Sasaki
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 240:427-34. 1996
    ..Since fibulin-2 is an abundant component of small and large blood vessels it could be a major target for proteolysis during vascular injuries...
  22. ncbi request reprint Mapping of the binding of platelet-derived growth factor to distinct domains of the basement membrane proteins BM-40 and perlecan and distinction from the BM-40 collagen-binding epitope
    W Göhring
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 255:60-6. 1998
    ..Neither nidogen, BM-40 or perlecan domain III-2 interfered with the binding of PDGF to its alpha and beta receptors, however, suggesting that these interactions may be mainly used for storage of PDGF in the extracellular matrix...
  23. ncbi request reprint Recombinant expression and structural and binding properties of alpha 1(VI) and alpha 2(VI) chains of human collagen type VI
    E Tillet
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 221:177-85. 1994
    ..Purified globular domains did not bind these ligands indicating the localization of binding sites within the triple-helical domain. Both chains showed a distinct affinity for heparin but failed to bind to various collagen types...
  24. ncbi request reprint Domain IVa of laminin alpha5 chain is cell-adhesive and binds beta1 and alphaVbeta3 integrins through Arg-Gly-Asp
    T Sasaki
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    FEBS Lett 509:181-5. 2001
    ..There it could play a role in angiogenesis steps requiring RGD-dependent integrins...
  25. ncbi request reprint Sequence, recombinant expression and tissue localization of two novel extracellular matrix proteins, fibulin-3 and fibulin-4
    R Giltay
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Matrix Biol 18:469-80. 1999
    ..A particularly prominent feature was the staining of variable sets of large and small blood vessels...
  26. ncbi request reprint Protein binding and cell adhesion properties of two laminin isoforms (AmB1eB2e, AmB1sB2e) from human placenta
    J C Brown
    Max Planck Institut fur Biochemie, Martinsried, Germany
    J Cell Sci 107:329-38. 1994
    ..No inhibition was observed with an Arg-Gly-Asp-containing peptide...
  27. ncbi request reprint Binding of nidogen and the laminin-nidogen complex to basement membrane collagen type IV
    M Aumailley
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    Eur J Biochem 184:241-8. 1989
    ..A more diverse collagen-IV-binding pattern was observed for the laminin nidogen complex, whereby interactions may involve both nidogen and short-arm structures of laminin...
  28. ncbi request reprint Modification of the laminin alpha 4 chain by chondroitin sulfate attachment to its N-terminal domain
    T Sasaki
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    FEBS Lett 505:173-8. 2001
    ..Antibodies to the fragment were useful to demonstrate a relatively high content of alpha 4 in several tissues and for the immunolocalization in various blood vessels, some basement membranes and interstitial regions...
  29. ncbi request reprint Binding of mouse and human fibulin-2 to extracellular matrix ligands
    T Sasaki
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    J Mol Biol 254:892-9. 1995
    ..This indicated multifunctional binding properties for fibulin-2 and several alternative routes for its integration into basement membranes and other extracellular structures...
  30. ncbi request reprint Structural characterization of recombinant domain II of the basement membrane proteoglycan perlecan
    M Costell
    Max Planck Institut fur Biochemie, Martinsried, Germany
    FEBS Lett 396:127-31. 1996
    ..The domain also showed N-glycosylation of a single acceptor site and 7-8 O-linked oligosaccharides. The latter were located mainly in the link region within proline-rich sequences...
  31. pmc Crystal structure and mapping by site-directed mutagenesis of the collagen-binding epitope of an activated form of BM-40/SPARC/osteonectin
    T Sasaki
    Max Planck Institut fur Biochemie, D 82152, Martinsried, Germany
    EMBO J 17:1625-34. 1998
    ..Selected mutations in the non-activated mutant DeltaI also reduced collagen binding, consistent with the same location of the epitope but in a more cryptic form in intact BM-40...
  32. ncbi request reprint Sequence and expression of a novel member (LTBP-4) of the family of latent transforming growth factor-beta binding proteins
    R Giltay
    Max Planck Institut fur Biochemie, Martinsried, Germany
    FEBS Lett 411:164-8. 1997
    ..The modular structure predicts that LTBP-4 should be a microfibrillar protein which probably also binds TGF-beta...
  33. ncbi request reprint Binding properties and protease stability of recombinant human nidogen
    U Mayer
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 227:681-6. 1995
    ..Since such degradation could be important for basement membrane remodelling, this difference between human and mouse may be biologically significant...
  34. ncbi request reprint Integrin and Arg-Gly-Asp dependence of cell adhesion to the native and unfolded triple helix of collagen type VI
    M Pfaff
    Max Planck Institut fur Biochemie, Frankfurt Hoechst, Germany
    Exp Cell Res 206:167-76. 1993
    ....
  35. ncbi request reprint Crystal structure of three consecutive laminin-type epidermal growth factor-like (LE) modules of laminin gamma1 chain harboring the nidogen binding site
    J Stetefeld
    Abteilungen für Strukturforschung and Proteinchemie, Max Planck Institut fur Biochemie, Martinsried, Germany
    J Mol Biol 257:644-57. 1996
    ..They also indicate that the crucial residues in the binding loops provide direct contacts with nidogen and explain the synergism between loops a and c which is essential for binding...
  36. ncbi request reprint Recombinant analysis of human alpha 1 (XVI) collagen. Evidence for processing of the N-terminal globular domain
    E Tillet
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 228:160-8. 1995
    ..Whether this process also occurs in situ remains to be shown...
  37. ncbi request reprint Drosophila laminin binds to mammalian nidogen and to heparan sulfate proteoglycan
    U Mayer
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 245:745-50. 1997
    ..The data suggest the existence of a nidogen analog and heparan sulfate proteoglycans in Drosophila, which remain to be identified...
  38. ncbi request reprint Structural properties of recombinant domain III-3 of perlecan containing a globular domain inserted into an epidermal-growth-factor-like motif
    B Schulze
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 231:551-6. 1995
    ..Together the data demonstrated a proper folding of recombinant domain III-3 similar to its structure in the native protein and provided the first structural evidence for a novel globular protein motif L4 based on an EGF-like scaffold...
  39. ncbi request reprint Structure and multiple conformations of the kunitz-type domain from human type VI collagen alpha3(VI) chain in solution
    M Zweckstetter
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Structure 4:195-209. 1996
    ..We have undertaken the determination of the three-dimensional (3D) structure of domain C5 in solution, by nuclear magnetic resonance (NMR), in order to establish the structural basis for the properties of this protein...
  40. ncbi request reprint Comparison of disintegrins with limited variation in the RGD loop in their binding to purified integrins alpha IIb beta 3, alpha V beta 3 and alpha 5 beta 1 and in cell adhesion inhibition
    M Pfaff
    , Martinsried, Germany
    Cell Adhes Commun 2:491-501. 1994
    ..More remote regions of the disintegrins and alignment of disulfide bridges are therefore likely to contribute to their affinity and selectivity...
  41. ncbi request reprint Differential regulation of fibulin, tenascin-C, and nidogen expression during wound healing of normal and glucocorticoid-treated mice
    R Fassler
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    Exp Cell Res 222:111-6. 1996
    ..They also indicate that repair involves complex regulatory processes which are obviously different for each of the four proteins studied...
  42. ncbi request reprint Macromolecular organization of basement membranes
    R Timpl
    Department of Protein Chemistry, Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
    Curr Opin Cell Biol 8:618-24. 1996
    ..Novel insights into functions of basement membrane components have been obtained from gene-targeting experiments and studies of mutated genes identified in inherited disorders...
  43. ncbi request reprint Properties of the extracellular calcium binding module of the proteoglycan testican
    E Kohfeldt
    Max Planck Institut fur Biochemie, Martinsried, Germany
    FEBS Lett 414:557-61. 1997
    ..No clear evidence was obtained for collagen binding, indicating that EC modules found in different proteins may not share similar functions...
  44. pmc Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells
    K Mann
    Max Planck Institut fur Biochemie, Martinsried, FRG
    EMBO J 8:65-72. 1989
    ..The latter was shown to be functional in cell attachment to nidogen. Binding sites for laminin and collagen IV are present on the C-terminal globule but not yet precisely localized...
  45. ncbi request reprint Supramolecular assembly of basement membranes
    R Timpl
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Bioessays 18:123-32. 1996
    ..Thus many molecular interactions, of variable affinities, determine the final shape of basement membranes and their preferred subanatomical localization...
  46. pmc Lack of beta 1 integrin gene in embryonic stem cells affects morphology, adhesion, and migration but not integration into the inner cell mass of blastocysts
    R Fassler
    Max Planck Institute for Biochemistry, Martinsried, Germany
    J Cell Biol 128:979-88. 1995
    ..This will allow studies of the consequences of beta 1 integrin deficiency in several in vivo situations...
  47. ncbi request reprint Characterization of a novel calcium-binding 90-kDa glycoprotein (BM-90) shared by basement membranes and serum
    M Kluge
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    Eur J Biochem 193:651-9. 1990
    ..Thus, BM-90 appears to be a novel basement membrane protein whose functions remain to be studied...
  48. ncbi request reprint Biglycan organizes collagen VI into hexagonal-like networks resembling tissue structures
    Charlotte Wiberg
    Department of Cell and Molecular Biology, University of Lund, BMC, S 221 84 Lund, Sweden
    J Biol Chem 277:49120-6. 2002
    ..This was demonstrated by electron microscopy after negative staining of gold-labeled biglycan in aggregation experiments with collagen VI...
  49. ncbi request reprint Influence of endostatin on embryonic vasculo- and angiogenesis
    Annette Schmidt
    Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, Cologne, Germany
    Dev Dyn 230:468-80. 2004
    ..Endostatin modulates the embryonic vascular development by enhancing proliferation, migration, and apoptosis as well as by regulating morphogenesis...
  50. pmc Genetic heterogeneity of cutis laxa: a heterozygous tandem duplication within the fibulin-5 (FBLN5) gene
    Dessislava Markova
    Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Am J Hum Genet 72:998-1004. 2003
    ..The results demonstrate that a heterozygous mutation in fibulin-5 can cause cutis laxa and also suggest that fibulin-5 and elastin gene mutations are not the exclusive cause of the disease...
  51. ncbi request reprint Distinct requirements for heparin and alpha-dystroglycan binding revealed by structure-based mutagenesis of the laminin alpha2 LG4-LG5 domain pair
    Harald Wizemann
    Max Planck Institut fur Biochemie, D 82152, Martinsried, Germany
    J Mol Biol 332:635-42. 2003
    ..The results should prove useful for the molecular dissection of laminin-receptor interactions in vivo...
  52. ncbi request reprint Alternative splicing in the aggrecan G3 domain influences binding interactions with tenascin-C and other extracellular matrix proteins
    Joanna M Day
    The Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 279:12511-8. 2004
    ..Our findings suggest that alternative splicing in the aggrecan G3 domain may be a mechanism for modulating interactions and extracellular matrix assembly...
  53. ncbi request reprint Laminin gamma3 chain binds to nidogen and is located in murine basement membranes
    Nikolaus Gersdorff
    Department of Prosthodontics, University of Goettingen, D 37075 Goettingen, Germany
    J Biol Chem 280:22146-53. 2005
    ..Ultrastructural immunogold staining localized the gamma3 chain to basement membranes of these tissues...
  54. pmc Structural basis for Gas6-Axl signalling
    Takako Sasaki
    Max Planck Institut fur Biochemie, Martinsried, Germany
    EMBO J 25:80-7. 2006
    ..Specificity at the major contact is suggested to result from the segregation of charged and apolar residues to opposite faces of the newly formed beta-sheet...
  55. ncbi request reprint WARP is a novel multimeric component of the chondrocyte pericellular matrix that interacts with perlecan
    Justin M Allen
    Cell and Matrix Biology Research Unit, Murdoch Childrens Research Institute and Department of Paediatrics, University of Melbourne, Royal Children s Hospital, Flemington Road, Parkville, Victoria 3052, Australia
    J Biol Chem 281:7341-9. 2006
    ..We conclude that WARP forms macromolecular structures that interact with perlecan to contribute to the assembly and/or maintenance of "permanent" cartilage structures during development and in mature cartilages...
  56. ncbi request reprint A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization
    Naoyuki Kobayashi
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Biol Chem 282:11805-16. 2007
    ..Immunoelectron microscopy localized fibulin-4 and -5 to fibrillin microfibrils at distinct locations. Our studies suggest there are unique and redundant functions shared by these structurally related proteins...
  57. ncbi request reprint Binding of mouse nidogen-2 to basement membrane components and cells and its expression in embryonic and adult tissues suggest complementary functions of the two nidogens
    Katriina Salmivirta
    Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden
    Exp Cell Res 279:188-201. 2002
    ..These findings revealed extensive coregulation of nidogen-1 and -2 expression and much more complementary functions of the two nidogens than previously recognized...
  58. ncbi request reprint Crystal structure of a C-terminal fragment of growth arrest-specific protein Gas6. Receptor tyrosine kinase activation by laminin G-like domains
    Takako Sasaki
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Biol Chem 277:44164-70. 2002
    ..Mutagenesis of some residues in this patch reduces Gas6-LG binding to the extracellular domain of Axl as well as Axl activation in glioblastoma cells, identifying a component of the receptor-binding site of Gas6...
  59. pmc Gene structure and functional analysis of the mouse nidogen-2 gene: nidogen-2 is not essential for basement membrane formation in mice
    Jurgen Schymeinsky
    Department of Protein Chemistry, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Mol Cell Biol 22:6820-30. 2002
    ..Nidogen-2 deficiency does not lead to hemorrhages in mice as one may have expected. Our results show that nidogen-2 is not essential for basement membrane formation or maintenance...
  60. pmc Complete sequence, recombinant analysis and binding to laminins and sulphated ligands of the N-terminal domains of laminin alpha3B and alpha5 chains
    Jörg H O Garbe
    Max Planck Institut fur Biochemie, Klopferspitz 18a, D 82152 Munich, Germany
    Biochem J 362:213-21. 2002
    ..These antibodies also allowed the identification of a new laminin assembly form 5B consisting of alpha3B, beta3 and gamma2 chains...
  61. pmc The 1.9-A crystal structure of the noncollagenous (NC1) domain of human placenta collagen IV shows stabilization via a novel type of covalent Met-Lys cross-link
    Manuel E Than
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 99:6607-12. 2002
    ..This structure provides insights into NC1-related diseases such as Goodpasture and Alport syndromes...
  62. ncbi request reprint The type XIII collagen ectodomain is a 150-nm rod and capable of binding to fibronectin, nidogen-2, perlecan, and heparin
    Hongmin Tu
    Collagen Research Unit, Biocenter and Department of Medical Biochemistry and Molecular Biology, University of Oulu, Aapistie 7, FIN 90220 Oulu, Finland
    J Biol Chem 277:23092-9. 2002
    ....
  63. pmc Laminin alpha1 globular domains 4-5 induce fetal development but are not vital for embryonic basement membrane assembly
    Susanne Schéele
    Department of Cell and Molecular Biology, Lund University, 22184 Lund, Sweden
    Proc Natl Acad Sci U S A 102:1502-6. 2005
    ..Yet, stem cells could not develop into polarized epiblast cells. Thus, alpha1LG4-5 provides vital signals for the conversion of stem cells to polarized epithelium...
  64. ncbi request reprint Domain IV of mouse laminin beta1 and beta2 chains
    Takako Sasaki
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 269:431-42. 2002
    ..Immunogold staining localized both beta chains primarily to basement membranes of kidney, muscle and various other tissues...
  65. ncbi request reprint Disruption of perlecan binding and matrix assembly by post-translational or genetic disruption of dystroglycan function
    Motoi Kanagawa
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Roy J and Lucille A Carver College of Medicine, The University of Iowa, 400 Eckstein Medical Building, Iowa City, IA 52242, USA
    FEBS Lett 579:4792-6. 2005
    ..These data suggest functional disruption of the trimolecular complex in glycosylation-deficient muscular dystrophy...
  66. ncbi request reprint The leucine-rich repeat protein PRELP binds perlecan and collagens and may function as a basement membrane anchor
    Eva Bengtsson
    Department of Cell and Molecular Biology, Section for Connective Tissue Biology, Lund University, SE 221 84 Lund, Sweden
    J Biol Chem 277:15061-8. 2002
    ..Based on the location of PRELP and its interaction with perlecan heparan sulfate chains and collagen, we propose a function of PRELP as a molecule anchoring basement membranes to the underlying connective tissue...
  67. ncbi request reprint Chondroitin sulfate perlecan enhances collagen fibril formation. Implications for perlecan chondrodysplasias
    Alexander J Kvist
    Department of Experimental Medical Sciences, Lund University, SE 22184 Lund, Sweden
    J Biol Chem 281:33127-39. 2006
    ..These findings demonstrate a role for perlecan chondroitin sulfate side chains in cartilage extracellular matrix assembly and provide an explanation for the perlecan-null chondrodysplasia...
  68. pmc Chondrogenic activity of the heparan sulfate proteoglycan perlecan maps to the N-terminal domain I
    Margaret M French
    Graduate School of Biomedical Sciences, University of Texas, Houston, USA
    J Bone Miner Res 17:48-55. 2002
    ..Thus, this matrix proteoglycan (PG) found at sites of cartilage formation in vivo is likely to enhance early stage differentiation induced by soluble chondrogenic factors...
  69. ncbi request reprint The minimal active domain of endostatin is a heparin-binding motif that mediates inhibition of tumor vascularization
    Anna Karin Olsson
    Department of Genetics and Pathology, Uppsala University, Rudbeck Laboratory, Uppsala, Sweden
    Cancer Res 64:9012-7. 2004
    ..Substitution of arginine residues 193/194 for alanine attenuates the antiangiogenic effects of the peptide. These data show an essential role for heparin binding in the antiangiogenic action of endostatin...
  70. ncbi request reprint Evidence of nidogen-2 compensation for nidogen-1 deficiency in transgenic mice
    Nicolai Miosge
    Department of Histology, Georg August Universitat Gottingen, D 37075, Gottingen, Germany
    Matrix Biol 21:611-21. 2002
    ..The data support the nidogen-2 compensation hypothesis to explain the limited phenotype observed following elimination of the nidogen-1 gene...
  71. ncbi request reprint Functional studies on recombinant domains of Mac-2-binding protein
    Simon Hellstern
    Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    J Biol Chem 277:15690-6. 2002
    ..Both fragments showed no cell adhesive activity in contrast to native M2BP, suggesting that a concerted binding action and/or multivalent interactions of rings are necessary for cell attachment...
  72. ncbi request reprint Targeted inactivation of murine laminin gamma2-chain gene recapitulates human junctional epidermolysis bullosa
    Xianmin Meng
    Department of Dermatology and Cutaneous Biology, Jefferson Medical College, and Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Invest Dermatol 121:720-31. 2003
    ..In summary, Lamc2-/- mouse recapitulates human JEB and provides novel insight into the role of laminin 5 in keratinocyte biology...
  73. ncbi request reprint Mutations in COCH that result in non-syndromic autosomal dominant deafness (DFNA9) affect matrix deposition of cochlin
    Robert Grabski
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Hum Genet 113:406-16. 2003
    ....
  74. pmc Opposing roles of integrin alpha6Abeta1 and dystroglycan in laminin-mediated extracellular signal-regulated kinase activation
    Maria Ferletta
    Department of Cell and Molecular Biology, Lund University, Sweden
    Mol Biol Cell 14:2088-103. 2003
    ..We conclude that laminin-1 and -10/11 share the ability to induce ERK activation, that this is regulated by integrin alpha6Abeta1, and suggest a novel role for dystroglycan-binding laminin domains as suppressors of this activation...
  75. ncbi request reprint Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development
    Michael Willem
    Max Planck Institute for Biochemistry, Department of Protein Chemistry, 82152 Martinsried, Germany
    Development 129:2711-22. 2002
    ..These data demonstrate that an interaction between two basement membrane proteins is required for early kidney morphogenesis in vivo...
  76. ncbi request reprint Homoassociation of VE-cadherin follows a mechanism common to "classical" cadherins
    Thomas Ahrens
    Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    J Mol Biol 325:733-42. 2003
    ..was found. Instead the data suggest that homoassociation of VE-cadherin follows the conserved mechanism of type I cadherins...
  77. ncbi request reprint Structure and function of collagen-derived endostatin inhibitors of angiogenesis
    Takako Sasaki
    Max Planck Institut fur Biochemie, Martinsried, Germany
    IUBMB Life 53:77-84. 2002
    ..Much remains to be learned, however, about details of the signal transduction cascades and how they interfere with pro-angiogenic factors under physiological conditions and during therapeutic treatment...
  78. pmc Mapping of SPARC/BM-40/osteonectin-binding sites on fibrillar collagens
    Camilla Giudici
    Max Planck Institut fur Biochemie, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Biol Chem 283:19551-60. 2008
    ..This sequence is conserved among alpha chains of collagens I, II, III, and V. In vitro collagen fibrillogenesis was delayed in the presence of SPARC, suggesting that SPARC might modulate collagen fibril assembly in vivo...