Petra Schwille

Summary

Affiliation: Technical University
Country: Germany

Publications

  1. ncbi Circular scanning fluorescence correlation spectroscopy on membranes
    Zdeněk Petrášek
    Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg, Dresden, Germany
    Opt Express 19:25006-21. 2011
  2. ncbi Photon arrival timing with sub-camera exposure time resolution in wide-field time-resolved photon counting imaging
    Zdeněk Petrášek
    Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg 47 49, 01307 Dresden, Germany
    Opt Express 18:24888-901. 2010
  3. ncbi Precise measurement of diffusion coefficients using scanning fluorescence correlation spectroscopy
    Zdeněk Petrášek
    Biophysics Group, Biotechnologisches Zentrum, Technische Universitat Dresden, Dresden, Germany
    Biophys J 94:1437-48. 2008
  4. ncbi Photobleaching in two-photon scanning fluorescence correlation spectroscopy
    Zdeněk Petrášek
    Biophysics Group, Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Chemphyschem 9:147-58. 2008
  5. ncbi Combined AFM and two-focus SFCS study of raft-exhibiting model membranes
    Salvatore Chiantia
    Biotechnologisches Zentrum, Technical University of Dresden, Tatzberg 47-51, 01307 Dresden, Germany
    Chemphyschem 7:2409-18. 2006
  6. ncbi Accurate determination of membrane dynamics with line-scan FCS
    Jonas Ries
    Technical University of Dresden, Biotechnologisches Zentrum, Dresden, Germany
    Biophys J 96:1999-2008. 2009
  7. ncbi Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules
    Shuizi Rachel Yu
    Developmental Genetics, Biotechnology Center, TUD, Tatzberg 47 49, 01307 Dresden, Germany
    Nature 461:533-6. 2009
  8. ncbi Geometry sensing by self-organized protein patterns
    Jakob Schweizer
    Biotechnology Center BIOTEC, Technische Universitat Dresden, Dresden, Germany
    Proc Natl Acad Sci U S A 109:15283-8. 2012
  9. ncbi How phospholipid-cholesterol interactions modulate lipid lateral diffusion, as revealed by fluorescence correlation spectroscopy
    Nicoletta Kahya
    Institute of Biophysics, Biotechnology Center, Dresden University of Technology, Tatzberg 47 49, D 01307 Dresden, Germany
    J Fluoresc 16:671-8. 2006
  10. ncbi Raft domain reorganization driven by short- and long-chain ceramide: a combined AFM and FCS study
    Salvatore Chiantia
    Biophysics Group, Biotechnologisches Zentrum BIOTEC, Technische Universitat, Tatzberg 47 51, 01307, Dresden, Germany
    Langmuir 23:7659-65. 2007

Collaborators

Detail Information

Publications73

  1. ncbi Circular scanning fluorescence correlation spectroscopy on membranes
    Zdeněk Petrášek
    Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg, Dresden, Germany
    Opt Express 19:25006-21. 2011
    ..Its robustness, short measurement times, and small size of the probed area makes this technique particularly attractive for analyzing the properties of membranes and molecules diffusing and interacting within them...
  2. ncbi Photon arrival timing with sub-camera exposure time resolution in wide-field time-resolved photon counting imaging
    Zdeněk Petrášek
    Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg 47 49, 01307 Dresden, Germany
    Opt Express 18:24888-901. 2010
    ..Possible applications include oxygen and ion concentration imaging using probes with luminescence lifetimes in the range of 100 ns to microseconds...
  3. ncbi Precise measurement of diffusion coefficients using scanning fluorescence correlation spectroscopy
    Zdeněk Petrášek
    Biophysics Group, Biotechnologisches Zentrum, Technische Universitat Dresden, Dresden, Germany
    Biophys J 94:1437-48. 2008
    ..With the help of simulations, we find the optimal value of the scan radius R for the experiment...
  4. ncbi Photobleaching in two-photon scanning fluorescence correlation spectroscopy
    Zdeněk Petrášek
    Biophysics Group, Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Chemphyschem 9:147-58. 2008
    ....
  5. ncbi Combined AFM and two-focus SFCS study of raft-exhibiting model membranes
    Salvatore Chiantia
    Biotechnologisches Zentrum, Technical University of Dresden, Tatzberg 47-51, 01307 Dresden, Germany
    Chemphyschem 7:2409-18. 2006
    ..Moreover we show how the combination of these different approaches is effective in reducing artifacts resulting from the use of a single technique...
  6. ncbi Accurate determination of membrane dynamics with line-scan FCS
    Jonas Ries
    Technical University of Dresden, Biotechnologisches Zentrum, Dresden, Germany
    Biophys J 96:1999-2008. 2009
    ..Furthermore, we probed the temperature dependence of the diffusion coefficient in several model membranes, and in human embryonic kidney cell membranes not affected by temperature-induced optical aberrations...
  7. ncbi Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules
    Shuizi Rachel Yu
    Developmental Genetics, Biotechnology Center, TUD, Tatzberg 47 49, 01307 Dresden, Germany
    Nature 461:533-6. 2009
    ..Our results demonstrate that a freely diffusing morphogen can set up concentration gradients in a complex multicellular tissue by a simple source-sink mechanism...
  8. ncbi Geometry sensing by self-organized protein patterns
    Jakob Schweizer
    Biotechnology Center BIOTEC, Technische Universitat Dresden, Dresden, Germany
    Proc Natl Acad Sci U S A 109:15283-8. 2012
    ..Our results give insight into the interplay between geometrical confinement and biochemical patterns emerging from a nonlinear reaction-diffusion system...
  9. ncbi How phospholipid-cholesterol interactions modulate lipid lateral diffusion, as revealed by fluorescence correlation spectroscopy
    Nicoletta Kahya
    Institute of Biophysics, Biotechnology Center, Dresden University of Technology, Tatzberg 47 49, D 01307 Dresden, Germany
    J Fluoresc 16:671-8. 2006
    ..FCS can be employed as a valuable tool to study lipid-sterol interactions and their effect on lipid dynamics, molecular packing and degree of conformational order...
  10. ncbi Raft domain reorganization driven by short- and long-chain ceramide: a combined AFM and FCS study
    Salvatore Chiantia
    Biophysics Group, Biotechnologisches Zentrum BIOTEC, Technische Universitat, Tatzberg 47 51, 01307, Dresden, Germany
    Langmuir 23:7659-65. 2007
    ..These differences may contribute to the explanation of the different physiological effects that are often observed for the long- and short-chain Cer...
  11. ncbi Plasma membranes are poised for activation of raft phase coalescence at physiological temperature
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Technical University of Dresden, Dresden, Germany
    Proc Natl Acad Sci U S A 105:10005-10. 2008
    ..Under this light, our induction of raft-phase separation suggests that plasma membrane composition is poised for selective and functional raft clustering at physiologically relevant temperature...
  12. ncbi Surface analysis of membrane dynamics
    Ana J García-Sáez
    Biophysics Group, BIOTEC, TU Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Biochim Biophys Acta 1798:766-76. 2010
    ..Then, we explain how they can be used to investigate lipid dynamics, the lateral organization of membrane domains and the dynamic structure of cellular membranes...
  13. ncbi Oligomerization and pore formation by equinatoxin II inhibit endocytosis and lead to plasma membrane reorganization
    Ana J García-Sáez
    BIOTEC der TU Dresden, 01307 Dresden, Germany
    J Biol Chem 286:37768-77. 2011
    ..We propose that plasma membrane reorganization into stabilized raft domains is part of the killing strategy of equinatoxin II...
  14. ncbi Functional convergence of hopanoids and sterols in membrane ordering
    James Peter Sáenz
    Max Plank Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 109:14236-40. 2012
    ..These observations suggest that the evolution of an ordered biochemically active liquid membrane could have evolved before the oxygenation of Earth's surface and the emergence of sterols...
  15. ncbi Min protein patterns emerge from rapid rebinding and membrane interaction of MinE
    Martin Loose
    Biophysics, BIOTEC, Dresden University of Technology, Dresden, Germany
    Nat Struct Mol Biol 18:577-83. 2011
    ....
  16. ncbi Fluorescence correlation spectroscopy for the study of membrane dynamics and organization in giant unilamellar vesicles
    Ana J García-Sáez
    BIOTEC, Technische Universitat Dresden, Germany
    Methods Mol Biol 606:493-508. 2010
    ..Finally, we present simple protocols for the preparation of GUVs and the acquisition and analysis of FCS data on the vesicles, so that diffusion coefficients of fluorescent probes within lipid membranes can be estimated...
  17. ncbi Elucidating membrane structure and protein behavior using giant plasma membrane vesicles
    Erdinç Sezgin
    Biophysics BIOTEC, Technische Universitat Dresden, Dresden, Germany
    Nat Protoc 7:1042-51. 2012
    ..GPMV preparation and isolation, including fluorescent labeling and observation, can be accomplished within 4 h...
  18. ncbi Fluorescence correlation studies of lipid domains in model membranes
    Nicoletta Kahya
    Institute of Biophysics, Biotechnology Center, Dresden University of Technology, Tatzberg, Germany
    Mol Membr Biol 23:29-39. 2006
    ..What we learn from investigations of lipid-lipid and lipid-protein interactions in simple model membranes can be regarded as an essential basic lecture for studies in more complex cellular membranes...
  19. ncbi Raft partitioning and dynamic behavior of human placental alkaline phosphatase in giant unilamellar vesicles
    Nicoletta Kahya
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden University of Technology, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Biochemistry 44:7479-89. 2005
    ..Finally, antibody-mediated cross-linking of PLAP induces a shift of its partition coefficient in favor of the l(o) phase...
  20. ncbi Role of ceramide in membrane protein organization investigated by combined AFM and FCS
    Salvatore Chiantia
    Biophysics Group, Biotechnologisches Zentrum BIOTEC, Technische Universitat Dresden, 01307, Germany
    Biochim Biophys Acta 1778:1356-64. 2008
    ..Analogue modulation of the local concentration and dynamics of membrane proteins/receptors by ceramide can be of crucial importance for the biological functions of cell membranes...
  21. ncbi Effect of temperature on the formation of liquid phase-separating giant unilamellar vesicles (GUV)
    Viktoria Betaneli
    BIOTEC, Biophysics Research Group, Technical University Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Chem Phys Lipids 165:630-7. 2012
    ....
  22. ncbi Single cell analysis of ligand binding and complex formation of interleukin-4 receptor subunits
    Thomas Weidemann
    Biophysics Research Group, Technische Universitat Dresden, Dresden, Germany
    Biophys J 101:2360-9. 2011
    ..This behavior may reflect hitherto undefined differences in the mode of receptor activation between type I (lymphoid) and type II (epithelial) receptor expressing cells...
  23. ncbi PI(4,5)P2 degradation promotes the formation of cytoskeleton-free model membrane systems
    Heiko Keller
    BIOTEC, Dresden University of Technology, Tatzberg 47 51, 01307 Dresden, Germany
    Chemphyschem 10:2805-12. 2009
    ..GPMVs differ from the physiological state of the plasma membrane in the presence of specific lipids, which limits their use as model systems. Furthermore, we propose that PI(4,5)P2 influences the phase-separation behavior...
  24. ncbi Fluorescence correlation spectroscopy for the study of membrane dynamics and protein/lipid interactions
    Ana J García-Sáez
    Department of Biotec, Biophysics Group, Biotechnologisches Zentrum, Tatzberg 47 51, TU Dresden, 1307 Dresden, Germany
    Methods 46:116-22. 2008
    ..Finally, we comment on some strategies recently developed for the specific improvement of FCS measurements on membranes...
  25. ncbi Pore formation by a Bax-derived peptide: effect on the line tension of the membrane probed by AFM
    Ana J García Sáez
    Biotechnologisches Zentrum der TU Dresden, Dresden, Germany
    Biophys J 93:103-12. 2007
    ..Such a decrease in line tension may be a general strategy of pore-forming peptides and proteins, as it affects the energetics of the pore and stabilizes the open state...
  26. ncbi Effects of ceramide on liquid-ordered domains investigated by simultaneous AFM and FCS
    Salvatore Chiantia
    Biotechnologisches Zentrum, Dresden University of Technology, Tatzberg, Dresden, Germany
    Biophys J 90:4500-8. 2006
    ..Formation of similar ceramide-enriched domains and, more general, alterations of lipid-lipid interactions can be of crucial importance for the biological function of cell membranes...
  27. ncbi Supercritical angle fluorescence correlation spectroscopy
    Jonas Ries
    Biotechnologisches Zentrum, Technical University of Dresden, Dresden, Germany
    Biophys J 94:221-9. 2008
    ..We present an extensive theoretical framework that allows for accurate and quantitative evaluation of the SA-FCS correlation curves...
  28. ncbi Near-critical fluctuations and cytoskeleton-assisted phase separation lead to subdiffusion in cell membranes
    Jens Ehrig
    Biophysics, BIOTEC, Technische Universitat Dresden, Dresden, Germany
    Biophys J 100:80-9. 2011
    ..Thus, we present a minimum realistic model for membrane rafts showing the features of both microscopic phase separation and subdiffusion...
  29. ncbi Biomimetic membrane systems to study cellular organization
    Martin Loose
    Dresden University of Technology, Tatzberg, Germany
    J Struct Biol 168:143-51. 2009
    ..We also summarize advantages and disadvantages of supported lipid bilayers as model membrane...
  30. ncbi Single molecule techniques for the study of membrane proteins
    Ana J García Sáez
    Biophysics Group, Biotechnologisches Zentrum BIOTEC der TU Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Appl Microbiol Biotechnol 76:257-66. 2007
    ..We will discuss the most relevant findings made during the recent years and their contribution to the membrane protein field...
  31. ncbi Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform
    Markus Burkhardt
    Biophysics Institute BioTec, Dresden University of Technology, Tatzberg 47 51, 01307 Dresden, Germany
    Opt Lett 30:2266-8. 2005
    ..Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complexbiomolecular dynamics on a single molecule level...
  32. ncbi Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells
    Thomas Ohrt
    BIOTEC, Biophysics, Dresden University of Technology, Dresden, Germany
    Nucleic Acids Res 36:6439-49. 2008
    ..Together, these results suggest a new RISC shuttling mechanism between nucleus and cytoplasm ensuring concomitant gene regulation by small RNAs in both compartments...
  33. ncbi Model membrane platforms to study protein-membrane interactions
    Erdinç Sezgin
    Biophysics BIOTEC, TU Dresden, Tatzberg, Dresden, Germany
    Mol Membr Biol 29:144-54. 2012
    ....
  34. ncbi Fluorescence correlation spectroscopy relates rafts in model and native membranes
    Kirsten Bacia
    Dresden University of Technology, Department of Biophysics, c/o Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Biophys J 87:1034-43. 2004
    ..Thus, fluorescence correlation spectroscopy promises to be a valuable tool to elucidate lipid raft associations in native cells and to gain deeper insight into the correspondence between model and natural membranes...
  35. ncbi Independence of maximum single molecule fluorescence count rate on the temporal and spectral laser pulse width in two-photon FCS
    Jörg Mütze
    Biophysics Group, Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg 47 51, Dresden, Germany
    J Fluoresc 17:805-10. 2007
    ..As a practical consequence, elaborate optimization of temporal and spectral laser pulse width, e.g. by introducing pulse stretchers in the beam path, is less critical than previously expected...
  36. ncbi Sterol structure determines the separation of phases and the curvature of the liquid-ordered phase in model membranes
    Kirsten Bacia
    Department of Biophysics, Dresden University of Technology, Tatzberg 47-51, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 102:3272-7. 2005
    ..Our findings could have implications for the role of sterols in various cell-biological processes such as budding of secretory vesicles, endocytosis, or formation of multivesicular bodies...
  37. ncbi Characterization of protein dynamics in asymmetric cell division by scanning fluorescence correlation spectroscopy
    Zdeněk Petrášek
    Biophysics Group, Biotechnologisches Zentrum, Technische Universitat Dresden, Dresden, Germany
    Biophys J 95:5476-86. 2008
    ....
  38. ncbi Stability of lipid domains
    Ana J García-Sáez
    Biophysics Group, BIOTEC, TU Dresden, Dresden, Germany
    FEBS Lett 584:1653-8. 2010
    ..In this review, we describe the current knowledge of the chemical and physical basis of lipid domains and its application to biological membranes...
  39. ncbi Analysis of prototype foamy virus particle-host cell interaction with autofluorescent retroviral particles
    Kristin Stirnnagel
    Institut fur Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universitat Dresden, Dresden, Germany
    Retrovirology 7:45. 2010
    ..Second, the unusually broad host range of FV is thought to be due to use of a very common entry receptor present on host cell plasma membranes, because all cell lines tested in vitro so far are permissive...
  40. ncbi Scanning FCS for the characterization of protein dynamics in live cells
    Zdeněk Petrášek
    BIOTEC, TU Dresden, Dresden, Germany
    Methods Enzymol 472:317-43. 2010
    ....
  41. ncbi Differential lipid packing abilities and dynamics in giant unilamellar vesicles composed of short-chain saturated glycerol-phospholipids, sphingomyelin and cholesterol
    Nicoletta Kahya
    Dresden University of Technology, c/o Max Planck Institute of Molecular Cell Biology and Genetics, Pftotenhauerstrasse 108, 01307 Dresden, Germany
    Chem Phys Lipids 135:169-80. 2005
    ..Finally, lipid dynamics in distinct phases is very low and astonishingly similar, thereby suggesting the poor ability of cholesterol in sorting short-chain saturated glycero-phospholipids and sphingomyelin...
  42. ncbi Efficient inhibition of the Alzheimer's disease beta-secretase by membrane targeting
    Lawrence Rajendran
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr 108, 01307 Dresden, Germany
    Science 320:520-3. 2008
    ..In addition to effectively targeting beta-secretase, this strategy could also be used in designing potent drugs against other membrane protein targets...
  43. ncbi Total internal reflection fluorescence correlation spectroscopy: effects of lateral diffusion and surface-generated fluorescence
    Jonas Ries
    Biotechnologisches Zentrum, Technical University of Dresden, Dresden, Germany
    Biophys J 95:390-9. 2008
    ..In addition, we consider the effects of surface-generated emission in objective-based TIR-FCS. The expressions for correlation functions presented here will facilitate quantitative and accurate measurements with TIR-FCS...
  44. ncbi Quantifying lipid diffusion by fluorescence correlation spectroscopy: a critical treatise
    Fabian Heinemann
    Biophysics Institute, BIOTEC Technische Universität Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Langmuir 28:13395-404. 2012
    ..Moreover, the comparability of the methods also in the case of slow diffusion was verified by measuring diffusion coefficients of DiD in GUVs consisting of DOPC and cholesterol...
  45. ncbi Efficient electroformation of supergiant unilamellar vesicles containing cationic lipids on ITO-coated electrodes
    Christoph Herold
    Biophysics, BIOTEC, Technische Universitat Dresden, Dresden, Germany
    Langmuir 28:5518-21. 2012
    ..We find that mild annealing of the ITO-coated surface in air reverts the effects of aging and ensures efficient reproducible electroformation of supergiant (diameter > 100 μm) unilamellar vesicles containing cationic lipids...
  46. ncbi The role of lipids in VDAC oligomerization
    Viktoria Betaneli
    Biophysics, BIOTEC, Technische Universitat Dresden, Dresden, Germany
    Biophys J 102:523-31. 2012
    ..We suggest that the specific lipid composition of the outer mitochondrial membrane might be of crucial relevance and, thus, a potential cue for regulating the oligomeric state of VDAC...
  47. ncbi Long-range transport of giant vesicles along microtubule networks
    Christoph Herold
    Biophysics, BIOTEC, Technische Universitat Dresden, Tatzberg 47 49, 01307 Dresden, Germany
    Chemphyschem 13:1001-6. 2012
    ..It may also find applications in bionanotechnology, where the controlled long-range transport of artificial cargo is a promising means to advance current lab-on-a-chip systems...
  48. ncbi Practical guidelines for dual-color fluorescence cross-correlation spectroscopy
    Kirsten Bacia
    BIOTEC, Department of Biophysics, Dresden University of Technology, Tatzberg 47 51, D 01307 Dresden, Germany
    Nat Protoc 2:2842-56. 2007
    ..It is a technique that is expected to see new applications in various fields of biochemical and cell biological research...
  49. ncbi Studying slow membrane dynamics with continuous wave scanning fluorescence correlation spectroscopy
    Jonas Ries
    Technical University of Dresden, Dresden, Germany
    Biophys J 91:1915-24. 2006
    ....
  50. ncbi Dehydration damage of domain-exhibiting supported bilayers: an AFM study on the protective effects of disaccharides and other stabilizing substances
    Salvatore Chiantia
    Biotechnologisches Zentrum, Dresden University of Technology, Tatzberg 47-51, D-01307, Dresden, Germany
    Langmuir 21:6317-23. 2005
    ..Finally, in addition to confirming previous findings, our results provide further insight into damage and alteration of microdomains in membranes as a consequence of stressful drying conditions...
  51. ncbi Protein self-organization: lessons from the min system
    Martin Loose
    Biophysics, BIOTEC, Dresden University of Technology, Dresden, Germany
    Annu Rev Biophys 40:315-36. 2011
    ..We hypothesize that cooperative membrane binding and unbinding, e.g., as an energy-dependent switch, may act as an important regulatory mechanism for protein oscillations and pattern formation in the cell...
  52. ncbi Fluorescence correlation spectroscopy in living cells
    Sally A Kim
    Institute of Biophysics, Dresden University of Technology, Tatzberg 47 51, D 01307 Dresden, Germany
    Nat Methods 4:963-73. 2007
    ..A discussion of advantages and disadvantages of one-photon versus two-photon excitation for FCS is available in Supplementary Methods online...
  53. ncbi Equinatoxin II permeabilizing activity depends on the presence of sphingomyelin and lipid phase coexistence
    Peter Schön
    Biophysics Group, BIOTEC, TU Dresden, Dresden, Germany
    Biophys J 95:691-8. 2008
    ....
  54. ncbi New concepts for fluorescence correlation spectroscopy on membranes
    Jonas Ries
    Biotechnologisches Zentrum, TU Dresden, Tatzberg 47 51, Dresden, D 01307, Germany
    Phys Chem Chem Phys 10:3487-97. 2008
    ..We also show how surface confined FCS enables the study of membrane dynamics even in presence of a strong cytosolic background and how FCS with a variable detection area can reveal submicroscopic heterogeneities in cell membranes...
  55. ncbi Yeast lipids can phase-separate into micrometer-scale membrane domains
    Christian Klose
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    J Biol Chem 285:30224-32. 2010
    ..Taken together, these results provide a mechanistic explanation for lipid raft-dependent lipid and protein sorting in yeast...
  56. ncbi Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms
    Jonas Ries
    Biotechnology Center, Technical University of Dresden, Dresden, Germany
    Nat Methods 6:643-5. 2009
    ..We quantified the mobility of fibroblast growth factor receptors Fgfr1 and Fgfr4 in cell membranes of living zebrafish embryos and determined their in vivo binding affinities to their ligand Fgf8...
  57. ncbi Membrane promotes tBID interaction with BCL(XL)
    Ana J García-Sáez
    Biophysics Group, BIOTEC, TU Dresden, Dresden, Germany
    Nat Struct Mol Biol 16:1178-85. 2009
    ..Our findings imply an active role of the membrane in modulating the interactions between BCL2 proteins that has so far been underestimated...
  58. ncbi Membrane domain-disrupting effects of 4-substitued cholesterol derivatives
    Dolores C Carrer
    Biophysics Group, BIOTEC, TU Dresden, Tatzberg 47 51, Dresden, Germany
    Langmuir 24:8807-12. 2008
    ..In this case, the resulting membranes display a fluidity intermediate between those of liquid-disordered and liquid-ordered phases...
  59. ncbi Protein-membrane interactions: the virtue of minimal systems in systems biology
    Senthil Arumugam
    BIOTEC, Dresden University of Technology, Dresden, Germany
    Wiley Interdiscip Rev Syst Biol Med 3:269-80. 2011
    ..These studies contribute to a deeper understanding of protein interactomes involving membranes and complement other approaches of systems biology...
  60. ncbi Fluorescence correlation spectroscopy in membrane structure elucidation
    Salvatore Chiantia
    Biotechnologisches Zentrum BIOTEC, TU Dresden, Germany
    Biochim Biophys Acta 1788:225-33. 2009
    ..Finally, we report several examples of studies which apply FCS on both model and biological membranes to obtain interesting insight in the topic of lateral membrane organization...
  61. ncbi Fluorescence correlation spectroscopy: novel variations of an established technique
    Elke Haustein
    BIOTEC TU Dresden, Institute for Biophysics, D 01307 Dresden, Germany
    Annu Rev Biophys Biomol Struct 36:151-69. 2007
    ..Rather, it has proven to be a highly versatile technique that can easily be adapted to solve specific biological questions, and it continues to find exciting applications in biology and medicine...
  62. ncbi Triple-color coincidence analysis: one step further in following higher order molecular complex formation
    Katrin G Heinze
    Biophysics Institute/BioTec, Dresden University of Technology, Dresden, Germany
    Biophys J 86:506-16. 2004
    ..The theoretical concept of triple-color coincidence analysis is outlined in detail, along with an experimental demonstration of its principles utilizing a simple nucleic acid reaction system...
  63. ncbi A novel homogenous assay for topoisomerase II action and inhibition
    Michael Jahnz
    TU Dresden/BioTec, Institute of Biophysics, Tatzberg 47-51, 01307 Dresden, Germany
    Chembiochem 6:920-6. 2005
    ..Further development of the DC-FCCS-based assay and subsequent application in high-throughput drug screening of new anticancer drugs is proposed and discussed...
  64. ncbi Lipids as modulators of proteolytic activity of BACE: involvement of cholesterol, glycosphingolipids, and anionic phospholipids in vitro
    Lucie Kalvodova
    Max-Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, Germany
    J Biol Chem 280:36815-23. 2005
    ..We have identified 3 groups of lipids that stimulate proteolytic activity of BACE: 1) neutral glycosphingolipids (cerebrosides), 2) anionic glycerophospholipids, and 3) sterols (cholesterol)...
  65. ncbi Focus on composition and interaction potential of single-pass transmembrane domains
    Remigiusz Worch
    BIOTEC, Biophysics Research Group, Technical University Dresden, Dresden, Germany
    Proteomics 10:4196-208. 2010
    ..Computational analysis of the free energy gain resulting from TMD dimer formation strongly corroborates the experimental findings, potentially allowing in silico pre-screening of interacting pairs...
  66. ncbi FRET and FCS--friends or foes?
    Harekrushna Sahoo
    Department of Biophysics, Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg 47 49, Dresden 01307, Germany
    Chemphyschem 12:532-41. 2011
    ..In this minireview, we discuss the potential and the caveats of FCS combined with FRET and give a short record on successful and promising applications...
  67. ncbi Synthetic biology of minimal systems
    Petra Schwille
    Biophysics BIOTEC, TU Dresden, Dresden, Germany
    Crit Rev Biochem Mol Biol 44:223-42. 2009
    ..We will show that with recent advances in understanding of cytoskeletal and membrane elements, the time might have come to experimentally challenge the concept of a minimal cell...
  68. ncbi In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5
    Thomas Ohrt
    Institute for Biophysics, BIOTEC, Dresden University of Technology, Tatzberg 47-51, 01307 Dresden, Germany
    Nucleic Acids Res 34:1369-80. 2006
    ..Exportin 5 therefore represents a key point of intersection between the siRNA and miRNA pathways, and, as such, is of fundamental importance for the design and interpretation of RNA interference experimentation...
  69. ncbi Spatial regulators for bacterial cell division self-organize into surface waves in vitro
    Martin Loose
    Biotechnologisches Zentrum der Technischen Universität Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Science 320:789-92. 2008
    ..We present a reaction-diffusion model of the MinD and MinE dynamics that accounts for our experimental observations and also captures the in vivo oscillations...
  70. ncbi An ultrasensitive site-specific DNA recombination assay based on dual-color fluorescence cross-correlation spectroscopy
    Michael Jahnz
    TU Dresden/BioTec, Institute of Biophysics Tatzberg 47-51, 01307 Dresden, Germany
    Nucleic Acids Res 33:e60. 2005
    ....
  71. ncbi TIR-FCS: staying on the surface can sometimes be better
    Petra Schwille
    Institute for Biophysics/Biotec, Dresden University of Technology, Dresden, Germany
    Biophys J 85:2783-4. 2003
  72. ncbi Spontaneous stretching of DNA in a two-dimensional nanoslit
    Madhavi Krishnan
    Biotechnologisches Zentrum, Technische Universitat Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Nano Lett 7:1270-5. 2007
    ..The observations not only shed light on electrostatic interactions of charged soft matter with like-charged confining walls but also offer a new method to stretch DNA in solution...
  73. ncbi SNAREs prefer liquid-disordered over "raft" (liquid-ordered) domains when reconstituted into giant unilamellar vesicles
    Kirsten Bacia
    Dresden University of Technology, Institute of Biophysics, Tatzberg 47-51, 01307 Dresden, Germany
    J Biol Chem 279:37951-5. 2004
    ....