Olaf Stemmann

Summary

Country: Germany

Publications

  1. ncbi request reprint Rephrasing anaphase: separase FEARs shugoshin
    Olaf Stemmann
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany
    Chromosoma 113:409-17. 2005
  2. ncbi request reprint Mutual inhibition of separase and Cdk1 by two-step complex formation
    Ingo H Gorr
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 19:135-41. 2005
  3. pmc Securin is not required for chromosomal stability in human cells
    Katrin Pfleghaar
    Institute of Human Genetics, Technical University Munich, Munich, Germany
    PLoS Biol 3:e416. 2005
  4. ncbi request reprint Anaphase topsy-turvy: Cdk1 a securin, separase a CKI
    Olaf Stemmann
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Cell Cycle 5:11-3. 2006
  5. pmc Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes
    Ingo H Gorr
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Cell Biol 8:1035-7. 2006
  6. ncbi request reprint Phosphorylation-dependent binding of cyclin B1 to a Cdc6-like domain of human separase
    Dominik Boos
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Biol Chem 283:816-23. 2008
  7. doi request reprint Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle
    Henrik Daub
    Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 31:438-48. 2008
  8. doi request reprint Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division
    Lily Hui Ching Wang
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Sci 123:806-13. 2010
  9. ncbi request reprint The AAA-ATPase p97-Ufd1-Npl4 is required for ERAD but not for spindle disassembly in Xenopus egg extracts
    Simone Heubes
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 120:1325-9. 2007
  10. ncbi request reprint Protein phosphatase 2A and separase form a complex regulated by separase autocleavage
    Andrew J Holland
    Faculty of Life Sciences, Michael Smith Building, Oxford Road, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 282:24623-32. 2007

Collaborators

Detail Information

Publications13

  1. ncbi request reprint Rephrasing anaphase: separase FEARs shugoshin
    Olaf Stemmann
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany
    Chromosoma 113:409-17. 2005
    ....
  2. ncbi request reprint Mutual inhibition of separase and Cdk1 by two-step complex formation
    Ingo H Gorr
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 19:135-41. 2005
    ..This unanticipated function of separase is negatively regulated by securin but independent of separase's proteolytic activity...
  3. pmc Securin is not required for chromosomal stability in human cells
    Katrin Pfleghaar
    Institute of Human Genetics, Technical University Munich, Munich, Germany
    PLoS Biol 3:e416. 2005
    ..Our data demonstrate that securin is dispensable for chromosomal stability in human cells. We propose that human cells possess efficient mechanisms to compensate for the loss of genes involved in chromosome segregation...
  4. ncbi request reprint Anaphase topsy-turvy: Cdk1 a securin, separase a CKI
    Olaf Stemmann
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Cell Cycle 5:11-3. 2006
    ..Furthermore, an unanticipated ability of separase to inhibit Cdk1 suggests additional, nonproteolytic functions of separase...
  5. pmc Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes
    Ingo H Gorr
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Cell Biol 8:1035-7. 2006
    ..Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity...
  6. ncbi request reprint Phosphorylation-dependent binding of cyclin B1 to a Cdc6-like domain of human separase
    Dominik Boos
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Biol Chem 283:816-23. 2008
    ..This suggests that despite its in vivo relevance, it promotes complex formation indirectly, possibly by inducing a conformational change in full-length separase...
  7. doi request reprint Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle
    Henrik Daub
    Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 31:438-48. 2008
    ..These results provide a vastly extended knowledge base for functional studies on kinases and their regulation through site-specific phosphorylation...
  8. doi request reprint Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division
    Lily Hui Ching Wang
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Sci 123:806-13. 2010
    ..Our data suggest that centromere decatenation can occur only after separase activation and cohesin removal, providing a plausible explanation for the persistence of centromere threads after anaphase onset...
  9. ncbi request reprint The AAA-ATPase p97-Ufd1-Npl4 is required for ERAD but not for spindle disassembly in Xenopus egg extracts
    Simone Heubes
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 120:1325-9. 2007
    ..The role of p97 in spindle disassembly during meiotic exit should therefore be reconsidered...
  10. ncbi request reprint Protein phosphatase 2A and separase form a complex regulated by separase autocleavage
    Andrew J Holland
    Faculty of Life Sciences, Michael Smith Building, Oxford Road, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 282:24623-32. 2007
    ..Together these observations provide a new mechanistic insight into a physiological function for separase self-cleavage...
  11. doi request reprint Securin and not CDK1/cyclin B1 regulates sister chromatid disjunction during meiosis II in mouse eggs
    Ibtissem Nabti
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
    Dev Biol 321:379-86. 2008
    ..In contrast, securin morpholino knockdown specifically induced loss of sister attachment, that could be restored by securin cRNA rescue. During metII arrest separase appears primarily regulated by securin binding, not CDK1/cyclin B1...
  12. pmc Hsp90 enables Ctf13p/Skp1p to nucleate the budding yeast kinetochore
    Olaf Stemmann
    Biochemie Zentrum, Ruprecht Karls Universitat, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 99:8585-90. 2002
    ..skp1 mutants exhibit growth defects on media containing geldanamycin. A skp1 mutation together with Hsp90 mutations exhibits synthetic lethality. An Hsp90 mutant contains decreased levels of active Ctf13p/Skp1p...
  13. ncbi request reprint Domain structure of separase and its binding to securin as determined by EM
    Hector Viadiu
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 12:552-3. 2005
    ..Based on labeling data and sequence analysis, we propose a model for the structure of separase, consisting of 26 ARM repeats, an unstructured region of 280 residues and two caspase-like domains, with securin binding to the ARM repeats...