G A Sprenger

Summary

Country: Germany

Publications

  1. pmc Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
    G A Sprenger
    Institut für Biotechnologie 1 des Forschungszentrums Jülich, Germany
    Proc Natl Acad Sci U S A 94:12857-62. 1997
  2. pmc Transaldolase B of Escherichia coli K-12: cloning of its gene, talB, and characterization of the enzyme from recombinant strains
    G A Sprenger
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Julich, Germany
    J Bacteriol 177:5930-6. 1995
  3. ncbi request reprint Zymomonas mobilis squalene-hopene cyclase gene (shc): cloning, DNA sequence analysis, and expression in Escherichia coli
    I G Reipen
    Institut fur Biotechnologie, Forschungszentrums Jülich GmbH, Germany
    Microbiology 141:155-61. 1995
  4. pmc Specificity of signal peptide recognition in tat-dependent bacterial protein translocation
    N Blaudeck
    , , , Germany
    J Bacteriol 183:604-10. 2001
  5. ncbi request reprint Identification of catalytically important residues in the active site of Escherichia coli transaldolase
    U Schörken
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
    Eur J Biochem 268:2408-15. 2001
  6. ncbi request reprint Crystallization and preliminary X-ray crystallographic studies of recombinant thermoresistant gluconate kinase GntK from Escherichia coli
    L Kraft
    Molecular Structural Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Acta Crystallogr D Biol Crystallogr 57:1159-61. 2001
  7. ncbi request reprint Characterization of a new feedback-resistant 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroF of Escherichia coli
    R Jossek
    Institut für Biotechnologie 1, Forschungszentrum Jülich GambH, Germany
    FEMS Microbiol Lett 202:145-8. 2001
  8. ncbi request reprint Crystal structures of the precursor form of glucose-fructose oxidoreductase from Zymomonas mobilis and its complexes with bound ligands
    D Nurizzo
    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    Biochemistry 40:13857-67. 2001
  9. ncbi request reprint Fully integrated L-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli
    N Rüffer
    Institute of Biotechnology, Forschungszentrum Julich GmbH, 52425 Juelich, Germany
    Bioprocess Biosyst Eng 26:239-48. 2004
  10. ncbi request reprint Fructose-6-phosphate aldolase is a novel class I aldolase from Escherichia coli and is related to a novel group of bacterial transaldolases
    M Schurmann
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, D 52425 Julich, Germany
    J Biol Chem 276:11055-61. 2001

Collaborators

  • T P Begley
  • R Takors
  • J Jia
  • U Schörken
  • M Schurmann
  • M Oldiges
  • N Rüffer
  • G Schneider
  • Y Lindqvist
  • D Nurizzo
  • L Kraft
  • R Jossek
  • N Blaudeck
  • R Freudl
  • I Kretzers
  • D Degenring
  • M Kunze
  • U Heidersdorf
  • L Raeven
  • M Klein
  • T Wiegert
  • S Thorell
  • E N Baker
  • H Sahm
  • J Bongaerts
  • D Halbig
  • I G Reipen
  • W Huang
  • K Poralla

Detail Information

Publications18

  1. pmc Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
    G A Sprenger
    Institut für Biotechnologie 1 des Forschungszentrums Jülich, Germany
    Proc Natl Acad Sci U S A 94:12857-62. 1997
    ....
  2. pmc Transaldolase B of Escherichia coli K-12: cloning of its gene, talB, and characterization of the enzyme from recombinant strains
    G A Sprenger
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Julich, Germany
    J Bacteriol 177:5930-6. 1995
    ..Fructose acted as a C(3) donor at a high apparent K(m) (>/=M) and with a V(rel) of 12%. The enzyme was inhibited by Tris-HCl, phosphate, or sugars with the L configuration at C(2) (L-glyceraldehyde, D-arabinose-5-phosphate)...
  3. ncbi request reprint Zymomonas mobilis squalene-hopene cyclase gene (shc): cloning, DNA sequence analysis, and expression in Escherichia coli
    I G Reipen
    Institut fur Biotechnologie, Forschungszentrums Jülich GmbH, Germany
    Microbiology 141:155-61. 1995
    ..These regions contained the core motif Gln-X-X-X-Gly-X-Trp...
  4. pmc Specificity of signal peptide recognition in tat-dependent bacterial protein translocation
    N Blaudeck
    , , , Germany
    J Bacteriol 183:604-10. 2001
    ..Such a situation is in marked contrast to the situation that is known to exist for Sec-dependent protein translocation...
  5. ncbi request reprint Identification of catalytically important residues in the active site of Escherichia coli transaldolase
    U Schörken
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
    Eur J Biochem 268:2408-15. 2001
    ....
  6. ncbi request reprint Crystallization and preliminary X-ray crystallographic studies of recombinant thermoresistant gluconate kinase GntK from Escherichia coli
    L Kraft
    Molecular Structural Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Acta Crystallogr D Biol Crystallogr 57:1159-61. 2001
    ..1, c = 78.9 A (P2(1)2(1)2). In all three crystal forms, there are two molecules in the asymmetric unit; the different forms occur in the same crystallization drop. The crystals diffract to at least 2.0 A using synchrotron radiation...
  7. ncbi request reprint Characterization of a new feedback-resistant 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroF of Escherichia coli
    R Jossek
    Institut für Biotechnologie 1, Forschungszentrum Jülich GambH, Germany
    FEMS Microbiol Lett 202:145-8. 2001
    ..These results strongly suggest that the N-terminus of AroF is involved in the molecular interactions occurring in the feedback-inhibition mechanism...
  8. ncbi request reprint Crystal structures of the precursor form of glucose-fructose oxidoreductase from Zymomonas mobilis and its complexes with bound ligands
    D Nurizzo
    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    Biochemistry 40:13857-67. 2001
    ..In this conformational change, the Lys181 side chain moves closer to the nicotinamide ring, stabilized by its increased negative charge...
  9. ncbi request reprint Fully integrated L-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli
    N Rüffer
    Institute of Biotechnology, Forschungszentrum Julich GmbH, 52425 Juelich, Germany
    Bioprocess Biosyst Eng 26:239-48. 2004
    ..Thus, the ISPR approach is superior to the reference non-ISPR process and even offers extraction rates approximately three times higher than the published membrane-based process...
  10. ncbi request reprint Fructose-6-phosphate aldolase is a novel class I aldolase from Escherichia coli and is related to a novel group of bacterial transaldolases
    M Schurmann
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, D 52425 Julich, Germany
    J Biol Chem 276:11055-61. 2001
    ..Another fsa-related gene, talC of Escherichia coli, was shown to also encode fructose-6-phosphate aldolase activity and not a transaldolase as proposed earlier...
  11. pmc Location of the transketolase (tkt) gene on the Escherichia coli physical map
    G A Sprenger
    Institut für Biotechnologie 1 des Forschungszentrums Jülich GmbH, Germany
    J Bacteriol 174:1707-8. 1992
  12. pmc Crystal structure of the reduced Schiff-base intermediate complex of transaldolase B from Escherichia coli: mechanistic implications for class I aldolases
    J Jia
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Protein Sci 6:119-24. 1997
    ..The C3 hydroxyl group interacts with the side chain of Asp 17 and Asn 35. Based on the crystal structure of this complex a reaction mechanism for transaldolase is proposed...
  13. ncbi request reprint Cloning, nucleotide sequence, and functional expression of the Escherichia coli enolase (eno) gene in a temperature-sensitive eno mutant strain
    M Klein
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
    DNA Seq 6:351-5. 1996
    ..Expression of the eno gene under the control of the lac promoter/operator resulted in an IPTG-inducible production of enzymatically active enolase in wild-type and enolase mutant strains...
  14. ncbi request reprint Crystal structure of transaldolase B from Escherichia coli suggests a circular permutation of the alpha/beta barrel within the class I aldolase family
    J Jia
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Structure 4:715-24. 1996
    ....
  15. ncbi request reprint Genetics of pentose-phosphate pathway enzymes of Escherichia coli K-12
    G A Sprenger
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH
    Arch Microbiol 164:324-30. 1995
    ..In recent years, genes for all enzymes of the pathway have been cloned and sequenced. Isoenzymes have been found for transketolase (genes tktA and tktB), ribose-5-phosphate isomerase (rpiA and rpiB) and transaldolase (talA and talB)...
  16. ncbi request reprint Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various gram-positive bacteria
    G A Sprenger
    Forschungszentrum Julich GmbH, Institut für Biotechnologie 1, Germany
    Biochim Biophys Acta 1158:103-6. 1993
    ..High similarity to the Enzymes IIMtl of Escherichia coli K-12 (gene mtlA) and of Staphylococcus carnosus was detected, but the two genes did not complement mannitol-negative E. coli mutants without the use of a heterologous promoter...
  17. ncbi request reprint Nucleotide sequence of the Escherichia coli K-12 transketolase (tkt) gene
    G A Sprenger
    Forschungszentrum Julich GmbH, Institut für Biotechnologie 1, Julich, Germany
    Biochim Biophys Acta 1216:307-10. 1993
    ..The DNA-derived protein sequence showed 49.5% identical amino acid residues with the transketolase of Rhodobacter sphaeroides, 46.8% identity with the enzyme from Saccharomyces cerevisiae, and 28.9% identity with the human transketolase...
  18. ncbi request reprint Stimulation, monitoring, and analysis of pathway dynamics by metabolic profiling in the aromatic amino acid pathway
    M Oldiges
    Institute of Biotechnology, Forschungszentrum Julich GmbH, 52425 Julich, Germany
    Biotechnol Prog 20:1623-33. 2004
    ..The results underline the necessity to extend the focus of glucose pulse experiments by studying not only the central metabolism but also anabolic pathways...