Research Topics
Species | K SotlarSummaryCountry: Germany Publications
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Publications
Acute myeloid leukaemia with t(8;21) associated with "occult" mastocytosis. Report of an unusual case and review of the literatureH-W Bernd
Institute of Pathology, , Ratzeburger Allee 160, , Germany
J Clin Pathol 57:324-8. 2004....
Splenic mastocytosis: report of two cases and detection of the transforming somatic C-KIT mutation D816VFriedrich Wimazal
Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Vienna, Austria
Leuk Lymphoma 45:723-9. 2004..In summary, our data show that the spleen can be a primary site of disease in rare cases of SM. Mastocytosis should therefore be considered as a (rare) differential diagnosis in patients with splenomegaly of unknown etiology...
Breast cancer proteomics by laser capture microdissection, sample pooling, 54-cm IPG IEF, and differential iodine radioisotope detectionHans Neubauer
University Women's Hospital, , Germany
Electrophoresis 27:1840-52. 2006..This study demonstrates the potential of ProteoTope and LCM to enable extremely sensitive and precise differential analyses from well-defined primary clinical specimen...
[The interdisciplinary approach to improve treatment quality of prostate cancer. Optimized nerve sparing in radical prostatectomy]K D Sievert
Universitatsklinik fur Urologie, Universitatsklinikum Tubingen, Hoppe Seyler Strasse 3, 72076 Tubingen
Urologe A 46:1104-12. 2007..After sufficient oncological treatment of prostate cancer the life quality becomes most important. A multi disciplinary research network aims to optimize the diagnostics and the resulting treatment of prostate cancer...
Simultaneous adrenal and extra-adrenal myelolipoma - an uncommon incident: case report and review of the literatureDerek Zieker
Department of General, Visceral and Transplant Surgery, Comprehensive Cancer Center, Tuebingen, Germany
World J Surg Oncol 6:72. 2008..Other soft tissue tumours such as well-differentiated liposarcomas appear morphological almost identical. Preoperative imaging and especially biopsy are important tools to diagnose these lesions...
One-step detection of c-kit point mutations using peptide nucleic acid-mediated polymerase chain reaction clamping and hybridization probesKarl Sotlar
Institute of Pathology, University of Tubingen, Tubingen, Germany
Am J Pathol 162:737-46. 2003....
Detection of c-kit point mutation Asp-816 --> Val in microdissected pooled single mast cells and leukemic cells in a patient with systemic mastocytosis and concomitant chronic myelomonocytic leukemiaKarl Sotlar
Institute of Pathology, University of Tubingen, Liebermeisterstrasse 8, D 72076, Tubingen, Germany
Leuk Res 26:979-84. 2002....
Detection and typing of human papillomavirus by e6 nested multiplex PCRK Sotlar
Institute of Pathology, University of Tubingen, Liebermeisterstrasse 8, 72076 Tubingen, Germany
J Clin Microbiol 42:3176-84. 2004..9% (124 of 259) of the patients. In conclusion, the novel NMPCR method is a sensitive and useful tool for HPV DNA detection, especially when exact HPV genotyping and the identification of multiple HPV infections are required...
Systemic mastocytosis associated with chronic idiopathic myelofibrosis: a distinct subtype of systemic mastocytosis associated with a [corrected] clonal hematological non-mast [corrected] cell lineage disorder carrying the activating point mutations KITD8Karl Sotlar
Institute of Pathology, University of Tubingen, Liebermeisterstrasse 8, D 72076 Tubingen, Germany
J Mol Diagn 10:58-66. 2008..Altogether, these data suggest that KIT(D816V+) SM can co-exist with JAK2(V617F+) CIMF and that, in some of these SM-CIMF cases, the two mutations are present in the neoplastic cells of both disease components...
Detection of high-risk human papillomavirus E6 and E7 oncogene transcripts in cervical scrapes by nested RT-polymerase chain reactionKarl Sotlar
Institute of Pathology, University of Tuebingen, Germany
J Med Virol 74:107-16. 2004..Future applications of the assay described may include the monitoring of women in studies investigating antiviral treatment or vaccination...
The tryptase positive compact round cell infiltrate of the bone marrow (TROCI-BM): a novel histopathological finding requiring the application of lineage specific markersH P Horny
University of Lubeck, Institute of Pathology, Lubeck, Germany
J Clin Pathol 59:298-302. 2006....
Detection of human papillomavirus type 16 E6/E7 oncogene transcripts in dysplastic and nondysplastic cervical scrapes by nested RT-PCRK Sotlar
Institute for Pathology, University of Tubingen, Tubingen, Germany
Gynecol Oncol 69:114-21. 1998....
Differential diagnoses of systemic mastocytosis in routinely processed bone marrow biopsy specimens: a reviewH P Horny
Institute of Pathology, Ansbach, Germany
Pathobiology 77:169-80. 2010....
An unusual case of systemic mastocytosis associated with chronic lymphocytic leukaemia (SM-CLL)H P Horny
Institute of Pathology, University of Lubeck, Germany
J Clin Pathol 59:264-8. 2006..This report contributes to the differential diagnosis of ISM by demonstrating that such lymphocytic aggregates may be neoplastic...
"Occult" mastocytosis with activating c-kit point mutation evolving into systemic mastocytosis associated with plasma cell myeloma and secondary amyloidosisK Sotlar
Institute of Pathology, , , Germany
J Clin Pathol 59:875-8. 2006..This finding was preliminarily termed "occult" mastocytosis...
Systemic mastocytosis with associated clonal haematological non-mast cell lineage diseases: a histopathological challengeH P Horny
Institute of Pathology, University of Lubeck, D 23538 Lubeck, Germany
J Clin Pathol 57:604-8. 2004....
Human papillomavirus type 16-associated primary squamous cell carcinoma of the rectumK Sotlar
Institute of Pathology, University of Tubingen, Germany
Gastroenterology 120:988-94. 2001..In line with the well-established concept of HPV-associated anogenital carcinogenesis, our results strongly suggest an etiologic role of HPV-16 in the pathogenesis of the metaplasia-dysplasia-SCC sequence in the case described...
Fatal parvovirus B19-associated myocarditis clinically mimicking ischemic heart disease: an endothelial cell-mediated diseaseBurkhard D Bültmann
Institute of Pathology, University of Tubingen, Tubingen, Germany
Hum Pathol 34:92-5. 2003..We speculate that, due to the high viral load in cardiac ECs, PVB19 infection of endothelial cells was sufficient to induce impaired coronary microcirculation with secondary cardiac myocyte necrosis...
Does intensive histopathological workup by serial sectioning increase the detection of lymph node micrometastasis in patients with primary cutaneous melanoma?H J Blaheta
Department of Dermatology, Skin Cancer Program, Eberhard Karls University, Tuebingen, Germany
Melanoma Res 11:57-63. 2001..Patients with melanoma cells detected exclusively by RT-PCR, however, were shown to be at increased risk for tumour recurrence...
Influence of tumor biological factors on tumor cell dissemination in primary breast cancerT Fehm
Department of Obstetrics and Gynecology University of Tuebingen, Calwerstrasse 7, D 72076 Tuebingen, Germany
Anticancer Res 24:4211-6. 2004..The aim of this study was to identify those features of the primary tumor related to the presence of disseminated tumor cells in the BM...
Additional reverse transcription-polymerase chain reaction of peripheral slices is not superior to analysis of the central slice in sentinel lymph nodes from melanoma patientsH J Blaheta
Department of Dermatology, Skin Cancer Program, and Department of Pathology, Eberhard Karls University, Liebermeister Str 25, 72076 Tuebingen, Germany
Br J Dermatol 150:477-83. 2004..Reverse transcription-polymerase chain reaction (RT-PCR) has been used as a sensitive means of detecting tumour cells in SLNs...
[Paraganglioma in the area of the head and neck. A review of molecular genetic research]S Braun
HNO Universitätsklinik, Tubingen
HNO 52:11-7. 2004..Thus, SDHD is the first known tumor suppressor gene encoding a mitochondrial protein. In this article we summarize the current state of knowledge concerning the development of paragangliomas...
Examination of regional lymph nodes by sentinel node biopsy and molecular analysis provides new staging facilities in primary cutaneous melanomaH J Blaheta
Department of Dermatology, Skin Cancer Program, Eberhard Karls University, Tuebingen, Germany
J Invest Dermatol 114:637-42. 2000..These data may have an impact on future tumor classifications of primary cutaneous melanoma...
Lymph node micrometastases of cutaneous melanoma: increased sensitivity of molecular diagnosis in comparison to immunohistochemistryH J Blaheta
Department of Dermatology, Eberhard Karls University, Tuebingen, Germany
Int J Cancer 79:318-23. 1998..0001). Patients with nodal micrometastases identified exclusively by RT-PCR had significantly higher tumor thickness as compared to patients with negative results by RT-PCR (p < 0.01)...
Mastocytosis: state of the artHans Peter Horny
Institute of Pathology, Ansbach, Germany
Pathobiology 74:121-32. 2007....
CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimensKarl Sotlar
Institute of Pathology, , , Germany
Am J Surg Pathol 28:1319-25. 2004..CD25 therefore appears to be a reliable immunohistochemical marker for the discrimination of neoplastic from normal/reactive MCs, with potential as a diagnostic tool in SM...
Delineation of patterns of bone marrow mast cell infiltration in systemic mastocytosis: value of CD25, correlation with subvariants of the disease, and separation from mast cell hyperplasiaManuela Krokowski
Institute of Pathology, , , Germany
Am J Clin Pathol 124:560-8. 2005..Our data show that the infiltration pattern in SM correlates with the disease subtype and should be recognized as an important aspect in the histomorphologic evaluation of the bone marrow...
Quantification of tumor cell burden by analysis of single cell lymph node disaggregates in metastatic prostate cancerDavid Schilling
Department of Urology, Eberhard Karls University, Tuebingen, Germany
Prostate 70:1110-8. 2010..We present a novel technique (1) to detect early tumor cell dissemination and (2) to quantify the true tumor burden...
Metastasis of malignant melanoma in the ureter: possible algorithms for a therapeutic approachGeorgios Gakis
Department of Urology, University Hospital Tuebingen, Eberhard Karls University, Tuebingen, Germany
Int J Urol 16:407-9. 2009..Based on this case as well as on recent published reports, we propose a possible algorithm for the treatment of metastatic melanoma of the upper urinary tract...
Parvovirus B19: a pathogen responsible for more than hematologic disordersBurkhard D Bültmann
Institute of Pathology, University of Tubingen, Liebermeisterstrasse 8, D 72076 Tubingen, Germany
Virchows Arch 442:8-17. 2003..Because treatment of lytic PVB19 infection has been successfully applied, the pathologist should be alerted to include PVB19 into the diagnostic spectrum of viral disease, especially in immunocompromised patients...
Variable presence of KITD816V in clonal haematological non-mast cell lineage diseases associated with systemic mastocytosis (SM-AHNMD)Karl Sotlar
Institute of Pathology, University of Munich, Germany
J Pathol 220:586-95. 2010....
Modified concept for radioisotope-guided sentinel lymph node dissection in prostate cancerDavid Schilling
Department of Urology, Eberhard Karls University Tuebingen, Hoppe Seyler Str 3, 72076, Tuebingen, Germany
World J Urol 28:715-20. 2010..To present a modified concept for sentinel lymph node (SLN)-guided pelvic lymph node dissection in prostate cancer...
Evaluation of mast cell activation syndromes: impact of pathology and immunohistologyH P Horny
Institute of Pathology, Ludwig Maximilians University, Munich, Germany
Int Arch Allergy Immunol 159:1-5. 2012..When the activating point mutant KIT D816V is detectable but criteria for diagnosis of mastocytosis are not completely met, a so-called (mono)clonal MCAS as a subvariant of primary MCAS should be diagnosed...
Detection of melanoma cells in sentinel lymph nodes, bone marrow and peripheral blood by a reverse transcription-polymerase chain reaction assay in patients with primary cutaneous melanoma: association with Breslow's tumour thicknessH J Blaheta
Department of Dermatology, Skin Cancer Programme, Eberhard Karls University, Liebermeister Str 25, 72076 Tubingen, Germany
Br J Dermatol 145:195-202. 2001..Tyrosinase reverse transcription-polymerase chain reaction (RT-PCR) has been shown to be highly sensitive in detecting tumour cells in melanoma patients...
Progression-specific genes identified by expression profiling of matched ductal carcinomas in situ and invasive breast tumors, combining laser capture microdissection and oligonucleotide microarray analysisChristina S Schuetz
Department of Obstetrics and Gynecology, Microarray Facility, Department of Medical Genetics, University of Tuebingen, Tuebingen, Germany
Cancer Res 66:5278-86. 2006....
ERG protein expression and genomic rearrangement status in primary and metastatic prostate cancer--a comparative study of two monoclonal antibodiesM Braun
Institute of Pathology, University Hospital of Bonn, Bonn, Germany
Prostate Cancer Prostatic Dis 15:165-9. 2012..Furthermore, we assessed whether the ERG protein expression is conserved in lymph node and distant PCa metastases...
Simplified detection of microsatellite instability in colorectal cancer without the need for corresponding germline DNA analysisM Ebinger
Department of Haematology and Oncology, Children s Hospital, University of Tubingen, Tubingen, Germany
J Clin Pathol 59:1114-5. 2006..Thus, the use of the five mononucleotide repeats in combination with the modified assessment technique simplifies the assessment of MSI, while retaining the sensitivity of the Bethesda panel for the detection of high-frequency MSI...
Detection of melanoma cells displaying multiple genomic changes in histopathologically negative sentinel lymph nodesAnja Ulmer
, , , Germany
Clin Cancer Res 11:5425-32. 2005..It remains to be determined whether the detection and genomic characterization of isolated melanoma cells in sentinel lymph nodes will provide relevant prognostic information...
Morphologic, functional, and metabolic magnetic resonance imaging-guided prostate biopsy in a patient with prior negative transrectal ultrasound-guided biopsies and persistently elevated prostate-specific antigen levelsMatthias P Lichy
Department of Urology, Comprehensive Cancer Center Tübingen, Eberhard Karls Universitat Tubingen, Tubingen, Germany
Urology 69:1208.e5-8. 2007..Magnetic resonance imaging-guided prostate biopsy was successfully performed. All specimens taken from the lesion showed a Gleason 5 tubular adenocarcinoma with low proliferative activity...
Increase of bone marrow lymphocytes in systemic mastocytosis: reactive lymphocytosis or malignant lymphoma? Immunohistochemical and molecular findings on routinely processed bone marrow biopsy specimensH P Horny
Institute of Pathology, University of Lubeck, D 23538 Lubeck, Germany
J Clin Pathol 56:575-8. 2003....
Increased translocator protein (TSPO) mRNA levels in colon but not in rectum carcinomaI Königsrainer
Department of General, Visceral and Transplant Surgery, Tubingen University Hospital, Tubingen, Germany
Eur Surg Res 39:359-63. 2007..TSPO was shown to be overexpressed in malignant tumors and cancer cell lines, correlating with enhanced malignant behavior. The present study analyzed the role of TSPO in patients with colorectal carcinomas...
Expression of KIT (CD117) in renal cell carcinoma and renal oncocytomaStefan Kruger
Institute of Pathology, University of Schleswig Holstein, Campus Lubeck, Lubeck, Germany
Oncology 68:269-75. 2005..Our aim was to analyze KIT expression immunohistochemically in renal cell carcinomas (RCCs) and in oncocytomas...
Breast cancer proteomics reveals correlation between estrogen receptor status and differential phosphorylation of PGRMC1Hans Neubauer
Department of Obstetrics and Gynecology, University of Tuebingen, Calwerstrasse, Germany
Breast Cancer Res 10:R85. 2008..Breast tumors lacking the estrogen receptor-alpha (ER-alpha) have increased incidence of resistance to therapy and poorer clinical prognosis...
[Aleukemic mast cell leukemia (formerly: "malignant mastocytosis"): an extremely rare form of leukemia. A case report and simultaneously a contribution to revised classification of mastocytosis]Hans Peter Horny
Institut fur Pathologie, Medizinische Universität zu Lübeck MUL, Lubeck
Wien Klin Wochenschr 114:222-8. 2002....
Organization of human papillomavirus productive cycle during neoplastic progression provides a basis for selection of diagnostic markersKate Middleton
National Institute for Medical Research, Mill Hill, London
J Virol 77:10186-201. 2003..It appears from our work that the presence of such proteins in cells at the epithelial surface allows the severity of the underlying disease to be predicted and that markers of viral gene expression may improve cervical screening...
Life cycle heterogeneity in animal models of human papillomavirus-associated diseaseWoei Ling Peh
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
J Virol 76:10401-16. 2002..The persistence of abortive infections caused by CRPV offers advantages for the development of therapeutic vaccines...
Aleukemic mast cell leukemia with abnormal immunophenotype and c-kit mutation D816VFrank Noack
Department of Pathology, University of Luebeck, Luebeck, Germany
Leuk Lymphoma 45:2295-302. 2004..However, additional (but yet unknown) molecular (genetic) defects have to be considered to explain the extremely heterogenous clinical course in these patients...
Ph-Chromosome-positive chronic myeloid leukemia with associated bone marrow mastocytosisHermine Agis
Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Austria
Leuk Res 29:1227-32. 2005..The exact knowledge of the pathology and target-profile of the associated leukemias in SM have important therapeutic implications...
Inter-laboratory validation of PCR-based HPV detection in pathology specimensIver Petersen
Charite Campus Mitte, Institut fur Pathologie, Universitatsmedizin Berlin, Chariteplatz 1, 10117, Berlin, Germany
Virchows Arch 451:701-16. 2007..The initiative is intended to foster the quality assurance of molecular HPV analysis in pathology and its correlation with morphological changes...
KIT mutation in mast cells and other bone marrow hematopoietic cell lineages in systemic mast cell disorders: a prospective study of the Spanish Network on Mastocytosis (REMA) in a series of 113 patientsAndres C Garcia-Montero
Centro de Investigacion del Cancer, Campus Miguel de Unamuno, 37007 Salamanca, Spain
Blood 108:2366-72. 2006..These results would support the notion that KIT mutation is a hallmark of adult SM where it targets a pluripotent hematopoietic stem cell, and may contribute to explaining previously observed discrepancies in the literature...
[Molecular pathological analysis of neoplastic mast cells with regard to the actual WHO classification of mast cell neoplasias]Karl Sotlar
,
Verh Dtsch Ges Pathol 90:227-35. 2006..By establishing methods for the detection of c-kit codon 816 mutations in paraffin-embedded tissues, the pathologist holds a central position in the diagnosis of systemic mastocytoses...
Parvovirus B19Burkhard D Bültmann
N Engl J Med 350:2006-7; author reply 2006-7. 2004
The European Competence Network on Mastocytosis (ECNM)Peter Valent
Division of Hematology and Hemostaseology, Department of Internal Medicine I, Medical University of Vienna, Austria
Wien Klin Wochenschr 116:647-51. 2004..In addition, the ECNM is dedicated to provide the best available information about the disease to patients and physicians...
Effects of the CD33-targeted drug gemtuzumab ozogamicin (Mylotarg) on growth and mediator secretion in human mast cells and blood basophilsMaria Theresa Krauth
Department of Internal Medicine I, Division of Hematology and Hemostaseology, Vienna, Austria
Exp Hematol 35:108-16. 2007..We have recently shown that mast cells (MCs) and basophils (BAs) express CD33. In the present study, we investigated the effects of GO on growth and mediator secretion in MCs and BAs...
Reorganization of nuclear domain 10 induced by papillomavirus capsid protein l2Luise Florin
Institute for Medical Microbiology and Hygiene, University of Mainz, Mainz, 55101, Germany
Virology 295:97-107. 2002..The differential change in protein composition observed provides further evidence to suggest that the ND10-associated proteins are an important interface of viral life cycle and host cell...
Mastocytosis: pathology, genetics, and current options for therapyPeter Valent
Department of Internal Medicine I, Medical Univeristy of Vienna, Austria
Leuk Lymphoma 46:35-48. 2005..Examples include the use of STI571 in patients with SM plus hypereosinophilic syndrome (SM-HES) and the FIPL1/PDGFRA fusion gene target, or chemotherapy for eradication of AML in patients with SM-AML...
