P Schwille

Summary

Country: Germany

Publications

  1. ncbi Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution
    P Schwille
    Department of Biochemical Kinetics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 72:1878-86. 1997
  2. ncbi Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation
    P Schwille
    School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853 USA
    Biophys J 77:2251-65. 1999
  3. ncbi Analyzing single protein molecules using optical methods
    P Schwille
    Experimental Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Curr Opin Biotechnol 12:382-6. 2001
  4. ncbi Characterization of the photoconversion on reaction of the fluorescent protein Kaede on the single-molecule level
    P S Dittrich
    Max Planck Institute for Biophysical Chemistry, Experimental Biophysics Group, , Germany
    Biophys J 89:3446-55. 2005
  5. ncbi Light-induced flickering of DsRed provides evidence for distinct and interconvertible fluorescent states
    F Malvezzi-Campeggi
    Experimental Biophysics Group, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Biophys J 81:1776-85. 2001
  6. ncbi Accessing molecular dynamics in cells by fluorescence correlation spectroscopy
    P Dittrich
    Experimental Biophysics Group, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Biol Chem 382:491-4. 2001
  7. ncbi Fluorescence correlation spectroscopy and its potential for intracellular applications
    P Schwille
    Experimental Biophysics Group, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Cell Biochem Biophys 34:383-408. 2001
  8. ncbi Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes
    P Schwille
    Cornell University, School of Applied and Engineering Physics, Ithaca, New York 14853, USA
    Cytometry 36:176-82. 1999
  9. ncbi Enzyme assays for confocal single molecule spectroscopy
    M Jahnz
    Max Planck Institute for Biophysical Chemistry, Experimental Biophysics Group, Dep 082, Am Fassberg 11, 37077 Göttingen Germany
    Curr Pharm Biotechnol 5:221-9. 2004
  10. ncbi Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy
    U Kettling
    Department of Biochemical Kinetics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 95:1416-20. 1998

Collaborators

Detail Information

Publications14

  1. ncbi Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution
    P Schwille
    Department of Biochemical Kinetics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 72:1878-86. 1997
    ..The concentration of the reaction product can be directly determined from the cross-correlation amplitude...
  2. ncbi Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation
    P Schwille
    School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853 USA
    Biophys J 77:2251-65. 1999
    ..At comparable signal levels, 2PE minimizes photobleaching in spatially restrictive cellular compartments, thereby preserving long-term signal acquisition...
  3. ncbi Analyzing single protein molecules using optical methods
    P Schwille
    Experimental Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Curr Opin Biotechnol 12:382-6. 2001
    ..Another fascinating perspective is the identification and selection of single favorable variants from complex libraries of diverse biomolecules...
  4. ncbi Characterization of the photoconversion on reaction of the fluorescent protein Kaede on the single-molecule level
    P S Dittrich
    Max Planck Institute for Biophysical Chemistry, Experimental Biophysics Group, , Germany
    Biophys J 89:3446-55. 2005
    ..6 s(-1) for the complete photoconversion, which is much slower than the internal dynamics of the chromophores. No fluorescent intermediate states could be revealed...
  5. ncbi Light-induced flickering of DsRed provides evidence for distinct and interconvertible fluorescent states
    F Malvezzi-Campeggi
    Experimental Biophysics Group, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Biophys J 81:1776-85. 2001
    ..In close resemblance to GFP, this light-induced dynamic behavior implies that the chromophore is subject to conformational rearrangements upon population of the excited state...
  6. ncbi Accessing molecular dynamics in cells by fluorescence correlation spectroscopy
    P Dittrich
    Experimental Biophysics Group, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Biol Chem 382:491-4. 2001
    ..To study free diffusion, genetically encoded fluorescent labels such as green fluorescent protein (GFP) or DsRed are preferable since they are less likely to nonspecifically interact with cellular substructures...
  7. ncbi Fluorescence correlation spectroscopy and its potential for intracellular applications
    P Schwille
    Experimental Biophysics Group, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Cell Biochem Biophys 34:383-408. 2001
    ..As an interesting alternative to the confocal instrumentation, two-photon excitation will be introduced, offering a number of important advantages especially in cellular systems with high-noise and low-signal levels...
  8. ncbi Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes
    P Schwille
    Cornell University, School of Applied and Engineering Physics, Ithaca, New York 14853, USA
    Cytometry 36:176-82. 1999
    ....
  9. ncbi Enzyme assays for confocal single molecule spectroscopy
    M Jahnz
    Max Planck Institute for Biophysical Chemistry, Experimental Biophysics Group, Dep 082, Am Fassberg 11, 37077 Göttingen Germany
    Curr Pharm Biotechnol 5:221-9. 2004
    ..Finally, one part of this review addresses the aspects of bioconjugation and the basic requirements for proper labeling dyes in order to be compatible with single molecule fluorescent spectroscopy...
  10. ncbi Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy
    U Kettling
    Department of Biochemical Kinetics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 95:1416-20. 1998
    ....
  11. ncbi Fluorescence lifetime images and correlation spectra obtained by multidimensional time-correlated single photon counting
    W Becker
    Becker and Hickl GmbH, 12277 Berlin, Germany
    Microsc Res Tech 69:186-95. 2006
    ..We show that the same technique and the same hardware can be used for precision fluorescence decay analysis and fluorescence correlation spectroscopy (FCS) in selected spots of a sample...
  12. ncbi Single molecule fluorescence imaging of the photoinduced conversion and bleaching behavior of the fluorescent protein Kaede
    S P Schäfer
    Institute of Biophysics BIOTEC, TU Dresden, Tatzberg 47 51, D 01307 Dresden, Germany
    Microsc Res Tech 69:210-9. 2006
    ..Contrary to Ando et al. (Proc Natl Acad Sci 2002;99:12651-12656), we found a significant increase in green fluorescence emission upon illumination with 405-nm light, which is typical for GFP and related proteins...
  13. ncbi Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy
    G Meacci
    Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
    Phys Biol 3:255-63. 2006
    ..The implications of the measured values for the oscillation mechanism are discussed...
  14. ncbi Diffusion and segmental dynamics of double-stranded DNA
    E P Petrov
    Institute of Biophysics/BIOTEC, Dresden University of Technology, Tatzberg 47-51, 01307 Dresden, Germany
    Phys Rev Lett 97:258101. 2006
    ....