H Schneckenburger

Summary

Country: Germany

Publications

  1. ncbi request reprint Total internal reflectance fluorescence reader for selective investigations of cell membranes
    Thomas Bruns
    Hochschule Aalen, Institut fur Angewandte Forschung, Germany
    J Biomed Opt 11:34011. 2006
  2. ncbi request reprint Total internal reflection fluorescence microscopy: technical innovations and novel applications
    Herbert Schneckenburger
    Hochschule Aalen, Institut fur Angewandte Forschung, Beethovenstrasse 1, 73430 Aalen, Germany
    Curr Opin Biotechnol 16:13-8. 2005
  3. ncbi request reprint Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells
    Herbert Schneckenburger
    Fachhochschule Aalen, Institut fur Angewandte Forschung, Aalen, Germany
    Photochem Photobiol Sci 3:127-31. 2004
  4. ncbi request reprint Time-gated total internal reflection fluorescence spectroscopy (TG-TIRFS): application to the membrane marker laurdan
    H Schneckenburger
    Institut fur Lasertechnologien in der Medizin und Messtechnik an der Universitat Ulm, Ulm, Germany
    J Microsc 211:30-6. 2003
  5. ncbi request reprint Laser-assisted optoporation of single cells
    Herbert Schneckenburger
    Institut fur Angewandte Forschung, Fachhochschule Aalen, 73428 Aalen, Germany
    J Biomed Opt 7:410-6. 2002
  6. ncbi request reprint Time-resolved in situ measurement of mitochondrial malfunction by energy transfer spectroscopy
    H Schneckenburger
    Institut fur Lasertechnologien in der Medizin und Messtechnik an der Universitat Ulm, Germany
    J Biomed Opt 5:362-6. 2000
  7. ncbi request reprint Cell viability in optical tweezers: high power red laser diode versus Nd:YAG laser
    H Schneckenburger
    Institut fur Lasertechnologien in der Medizin und Messtechnik, Universitat Ulm, Germany
    J Biomed Opt 5:40-4. 2000
  8. ncbi request reprint Energy transfer spectroscopy for measuring mitochondrial metabolism in living cells
    H Schneckenburger
    Institut fur Lasertechnologien in der Medizin und Messtechnik, Universitat Ulm, Germany
    Photochem Photobiol 66:34-41. 1997
  9. ncbi request reprint Optical detection of mitochondrial NADH content in intact human myotubes
    M H Gschwend
    Abteilung für Allgemeine Physiologie, Universitat Ulm, Germany
    Cell Mol Biol (Noisy-le-grand) 47:OL95-104. 2001
  10. ncbi request reprint Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM): realization and application of a compact illumination device
    K Stock
    Institut fur Lasertechnologien in der Medizin und Messtechnik an der Universitat Ulm, Ulm, Germany
    J Microsc 211:19-29. 2003

Collaborators

Detail Information

Publications21

  1. ncbi request reprint Total internal reflectance fluorescence reader for selective investigations of cell membranes
    Thomas Bruns
    Hochschule Aalen, Institut fur Angewandte Forschung, Germany
    J Biomed Opt 11:34011. 2006
    ..In addition, particularly with regard to potential pharmaceutical applications, the kinetics of the intracellular translocation of a fluorescent protein kinase c fusion protein upon stimulation of the cells is determined...
  2. ncbi request reprint Total internal reflection fluorescence microscopy: technical innovations and novel applications
    Herbert Schneckenburger
    Hochschule Aalen, Institut fur Angewandte Forschung, Beethovenstrasse 1, 73430 Aalen, Germany
    Curr Opin Biotechnol 16:13-8. 2005
    ..In addition to conventional fluorescent dyes, genetically engineered fluorescent proteins are increasingly being used to measure molecular conformations or intermolecular distances by fluorescence resonance energy transfer...
  3. ncbi request reprint Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells
    Herbert Schneckenburger
    Fachhochschule Aalen, Institut fur Angewandte Forschung, Aalen, Germany
    Photochem Photobiol Sci 3:127-31. 2004
    ..Therefore, for diagnostic applications the concentration of R123 in the incubation medium should not be above 25 microM...
  4. ncbi request reprint Time-gated total internal reflection fluorescence spectroscopy (TG-TIRFS): application to the membrane marker laurdan
    H Schneckenburger
    Institut fur Lasertechnologien in der Medizin und Messtechnik an der Universitat Ulm, Ulm, Germany
    J Microsc 211:30-6. 2003
    ....
  5. ncbi request reprint Laser-assisted optoporation of single cells
    Herbert Schneckenburger
    Institut fur Angewandte Forschung, Fachhochschule Aalen, 73428 Aalen, Germany
    J Biomed Opt 7:410-6. 2002
    ..High transfection rates, visual control and universality towards various cell lines are possibly the main advantages of laser-assisted optoporation in comparison with presently existing methods of cell transfection...
  6. ncbi request reprint Time-resolved in situ measurement of mitochondrial malfunction by energy transfer spectroscopy
    H Schneckenburger
    Institut fur Lasertechnologien in der Medizin und Messtechnik an der Universitat Ulm, Germany
    J Biomed Opt 5:362-6. 2000
    ..Concomitantly the amount of mitochondrial NADH increased. Excellent linearity between the number of cells incubated with R123 and fluorescence intensity was found in suspension...
  7. ncbi request reprint Cell viability in optical tweezers: high power red laser diode versus Nd:YAG laser
    H Schneckenburger
    Institut fur Lasertechnologien in der Medizin und Messtechnik, Universitat Ulm, Germany
    J Biomed Opt 5:40-4. 2000
    ..Two-photon excitation of NAD(P)H and flavins may also affect cell viability after exposure to 670-680 nm, whereas one-photon excitation of water molecules seems to limit cell viability at 1064 nm...
  8. ncbi request reprint Energy transfer spectroscopy for measuring mitochondrial metabolism in living cells
    H Schneckenburger
    Institut fur Lasertechnologien in der Medizin und Messtechnik, Universitat Ulm, Germany
    Photochem Photobiol 66:34-41. 1997
    ..Therefore, time-gated energy transfer spectroscopy is suggested to be an appropriate tool for probing mitochondrial malfunction...
  9. ncbi request reprint Optical detection of mitochondrial NADH content in intact human myotubes
    M H Gschwend
    Abteilung für Allgemeine Physiologie, Universitat Ulm, Germany
    Cell Mol Biol (Noisy-le-grand) 47:OL95-104. 2001
    ..We conclude that time-gated spectroscopy combined with non-radiative energy transfer is an appropriate tool for probing mitochondrial enzyme complex deficiencies...
  10. ncbi request reprint Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM): realization and application of a compact illumination device
    K Stock
    Institut fur Lasertechnologien in der Medizin und Messtechnik an der Universitat Ulm, Ulm, Germany
    J Microsc 211:19-29. 2003
    ..The compact illumination device is also suitable for combining TIRFM with further innovative techniques, e.g. time-resolved fluorescence spectroscopy, fluorescence lifetime imaging (FLIM) or fluorescence resonance energy transfer (FRET)...
  11. ncbi request reprint Plasma membrane associated location of sulfonated meso-tetraphenylporphyrins of different hydrophilicity probed by total internal reflection fluorescence spectroscopy
    R Sailer
    Institut fur Lasertechnologien in der Medizin und Messtechnik, Universitat Ulm, Germany
    Photochem Photobiol 71:460-5. 2000
    ..In conclusion, these results provide for the first time direct evidence for a plasma membrane-associated fraction of the hydrophilic compounds TPPS3 and TPPS4 in living cells...
  12. doi request reprint Light exposure and cell viability in fluorescence microscopy
    H Schneckenburger
    Hochschule Aalen, Institut fur Angewandte Forschung, Beethovenstr 1, 73430 Aalen, Germany
    J Microsc 245:311-8. 2012
    ..Tolerable light doses were also maintained in fluorescence microscopy of larger 3D samples, e.g. cell spheroids exposed to structured illumination, but may be exceeded in super-resolution microscopy based on single molecule detection...
  13. ncbi request reprint Laser-assisted fluorescence microscopy for measuring cell membrane dynamics
    Herbert Schneckenburger
    Fachhochschule Aalen, Institut fur Angewandte Forschung, Beethovenstr 1, 73430 Aalen, Germany
    Photochem Photobiol Sci 3:817-22. 2004
    ..Non-radiative energy transfer was used to detect laurdan selectively in close proximity to a molecular acceptor (DiI) and may present a possibility for measuring membrane dynamics in specific microenvironments...
  14. ncbi request reprint Autofluorescence lifetime imaging of cultivated cells using a UV picosecond laser diode
    Herbert Schneckenburger
    Fachhochschule Aalen, Institut fur Angewandte Forschung, Beethovenstr 1, 73430 Aalen, Germany
    J Fluoresc 14:649-54. 2004
    ..Although present applications are still limited by their low pulse energy (< or = 5 pJ), uv picosecond laser diodes have a large potential in high resolution fluorescence microscopy and fluorescence lifetime endoscopy...
  15. doi request reprint Forster resonance energy transfer-based total internal reflection fluorescence reader for apoptosis
    Thomas Bruns
    Hochschule Aalen, Institut fur Angewandte Forschung, Beethovenstrasse 1, 73430 Aalen, Germany
    J Biomed Opt 14:021003. 2009
    ..Therefore, this test system appears to be a sensitive device for the detection of apoptosis and more generally for drug screening or in vitro diagnosis on a nanometer scale...
  16. ncbi request reprint Relation between intracellular location and photodynamic efficacy of 5-aminolevulinic acid-induced protoporphyrin IX in vitro. Comparison between human glioblastoma cells and other cancer cell lines
    Reinhard Sailer
    Institut fur Lasertechnologien in der Medizin und Messtechnik an der Universitat Ulm, Helmholtzstr 12, 89081, Ulm, Germany
    Photochem Photobiol Sci 6:145-51. 2007
    ....
  17. ncbi request reprint Microfluorometry of cell membrane dynamics
    Petra Weber
    Hochschule Aalen, Institut fur Angewandte Forschung, 73430 Aalen, Germany
    Cytometry A 69:185-8. 2006
    ..Spectral properties are evaluated and displayed as microscopic images...
  18. doi request reprint Total internal reflection fluorescence lifetime and anisotropy screening of cell membrane dynamics
    Thomas Bruns
    Hochschule Aalen, Institut fur Angewandte Forschung, Beethovenstr 1, 73430 Aalen, Germany
    J Biomed Opt 13:041317. 2008
    ..For all measurements in this study, either human glioblastoma cells incubated with the fluorescent membrane marker NBD-cholesterol or human breast cancer cells expressing a membrane-associating fluorescent protein were used...
  19. ncbi request reprint Cell-substrate topology upon ALA-PDT using variable-angle total internal reflection fluorescence microscopy (VA-TIRFM)
    Henri Pierre Lassalle
    Hochschule Aalen, Institut fur Angewandte Forschung, Anton Huber Str 21, 73430 Aalen, Germany
    J Environ Pathol Toxicol Oncol 26:83-8. 2007
    ..Moreover, focal contacts visualized with a fusion protein of yellow fluorescent protein and focal adhesion kinase were maintained on light exposure, i.e., light-induced detachment of cells from their substrate was not likely to occur...
  20. doi request reprint Fluorescence imaging of Foscan and Foslip in the plasma membrane and in whole cells
    Henri Pierre Lassalle
    Hochschule Aalen, Anton Huber Strasse 21, 73430 Aalen, Germany
    J Photochem Photobiol B 92:47-53. 2008
    ..A combination of TIR and fluorescence lifetime imaging microscopy (FLIM), described for the first time, appears to be promising for understanding some key mechanisms of photodynamic therapy (PDT)...
  21. doi request reprint Impact of cholesterol level upon APP and BACE proximity and APP cleavage
    Christine A F von Arnim
    Deparment of Neurology, Ulm University, Albert Einstein Allee 11, 89081 Ulm, Germany
    Biochem Biophys Res Commun 370:207-12. 2008
    ..To analyze the impact of alterations in cholesterol level upon BACE-cleavage, we measured sAPP secretion. Alteration of APP processing and BACE proximity by cholesterol might be explained by alterations in cell membrane fluidity...