Research Topics
Genomes and Genes | Herbert SchmidtSummaryCountry: Germany Publications
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Publications
Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coliH Schmidt
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
Infect Immun 69:6863-73. 2001..We conclude that the newly described island occurs exclusively in a subgroup of STEC strains that are eae negative and contain the variant stx(2d )gene...
Antibody reactivity of a standardized human serum protein solution against a spectrum of microbial pathogens and toxins: comparison with fresh frozen plasmaHerbert Schmidt
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Würzburg and Biotest Pharma GmbH, Dreieich, Germany
Ther Apher 6:145-53. 2002..coli. By using 3 different HSP batches in parallel, we could demonstrate antibody reactivity against important microbial pathogens and toxins. This antibody profile is essentially more homogeneous than that of 3 batches of FFP...- Shiga-toxin-converting bacteriophagesH Schmidt
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
Res Microbiol 152:687-95. 2001..Stx phages play an important role in the expression of Stx and in lateral gene transfer and are therefore a contribution to the emergence of new Stx-producing E. coli (STEC) variants... - A gene cluster closely related to type II secretion pathway operons of gram-negative bacteria is located on the large plasmid of enterohemorrhagic Escherichia coli O157 strainsH Schmidt
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
FEMS Microbiol Lett 148:265-72. 1997..coli, enteroaggregative E. coli, enterotoxigenic E. coli and enteroinvasive E. coli. The results obtained with the etp probe were confirmed by a PCR approach to specifically detect an internal fragment of the etpD gene... - Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage phi3538 isolated from Escherichia coli O157:H7H Schmidt
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, 97080 Wurzburg, Germany
Appl Environ Microbiol 65:3855-61. 1999..coli and that the lysogens produced infectious phage progeny. Stx-encoding bacteriophages are able to spread stx genes among enteric E. coli strains... - Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonalityH Schmidt
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
J Infect Dis 179:115-23. 1999..Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7... - Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933H Schmidt
Institut für Hygiene und Mikrobiologie, Universität Wüzburg, Germany
Infect Immun 63:1055-61. 1995..The EHEC hemolysin appears to have clinical importance because it occurs in all O157 strains tested and is reactive to sera of patients with hemolytic uremic syndrome... - Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:h7H Schmidt
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
Microbiology 142:907-14. 1996..It was located close to the EHEC hly operon... - KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7W Brunder
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
Microbiology 142:3305-15. 1996..coli) revealed a close association between the occurrence of EHEC-haemolysin and the katP gene in Shiga-like-toxin-producing E. coli O157 strains... - Enterohemolytic phenotypes and genotypes of shiga toxin-producing Escherichia coli O111 strains from patients with diarrhea and hemolytic-uremic syndromeH Schmidt
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
J Clin Microbiol 34:2364-7. 1996.... - Antibacterials that are used as growth promoters in animal husbandry can affect the release of Shiga-toxin-2-converting bacteriophages and Shiga toxin 2 from Escherichia coli strainsB Kohler
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
Microbiology 146:1085-90. 2000..In vivo induction of Stx phages from lysogenic STEC may increase the amount of free phages in the intestine and therefore may contribute to the spread of STEC and development of new STEC pathotypes... - The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elementsW Brunder
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
Microbiology 145:1005-14. 1999..The EHEC-hlyA gene, however, is present in both. In conclusion, this study shows that the large plasmids of STEC are not uniform genetic elements but heterogeneous in both their gene composition and arrangement... - Cattle can be a reservoir of sorbitol-fermenting shiga toxin-producing Escherichia coli O157:H(-) strains and a source of human diseasesM Bielaszewska
Institute for Medical Microbiology, The 2nd Medical Faculty, Charles University, 150 06 Prague, Czech Republic
J Clin Microbiol 38:3470-3. 2000..This is the first evidence that cattle can be a reservoir of SF STEC O157:H(-) and a source of human diseases... - A genomic island, termed high-pathogenicity island, is present in certain non-O157 Shiga toxin-producing Escherichia coli clonal lineagesH Karch
Institut für Hygiene und Mikrobiologie, D 97080 Wurzburg, 97070 Wurzburg, Germany
Infect Immun 67:5994-6001. 1999..The hypothesis that the HPI in STEC contributes to the fitness of the strains in certain ecological niches rather than to their pathogenic potential is discussed... - Structure and function of plasmid pColD157 of enterohemorrhagic Escherichia coli O157 and its distribution among strains from patients with diarrhea and hemolytic-uremic syndromeC Hofinger
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
J Clin Microbiol 36:24-9. 1998..coli O157 strains isolated between 1987 and 1991 harbored plasmid pColD157, only 1 of 50 strains isolated during 1996 carried this plasmid. In addition, all strains harboring plasmid pColD157 were shown to have colicinogenic activity... - The nucleotide sequence of Shiga toxin (Stx) 2e-encoding phage phiP27 is not related to other Stx phage genomes, but the modular genetic structure is conservedJürgen Recktenwald
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
Infect Immun 70:1896-908. 2002..Although the overall DNA sequence of phi P27 is not highly related to other known phages, the data obtained demonstrate a typical lambdoid genome structure... - Heterogeneity of the prtC gene of Porphyromonas gingivalisM Wittstock
Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Bau 17, Josef Schneider Str 2, D 97080 Wurzburg, Germany
Oral Microbiol Immunol 15:33-9. 2000..The observed heterogeneity of prtC genes may be a result of microevolution processes... - Genetic analysis of enteropathogenic and enterohemorrhagic Escherichia coli serogroup O103 strains by molecular typing of virulence and housekeeping genes and pulsed-field gel electrophoresisLothar Beutin
Division of Microbial Toxins, Department of Biological Safety, Robert Koch Institute, Berlin, Germany
J Clin Microbiol 43:1552-63. 2005..Novel virulence markers, such as efa (EHEC factor for adherence), paa (porcine adherence factor), and cif (cell cycle-inhibiting factor), were found widely associated with E. coli O103 EPEC and STEC strains... - Complete sequence of the large virulence plasmid pSFO157 of the sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- strain 3072/96Werner Brunder
, Josef-Schneider-Strasse 2, , Germany
Int J Med Microbiol 296:467-74. 2006..coli O157 virulence plasmids have a complex evolutionary origin... - Hemolysin from Shiga toxin-negative Escherichia coli O26 strains injures microvascular endotheliumThomas Aldick
Institute of Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University of Munster, Robert Koch Str 41, 48149 Munster, Germany
Microbes Infect 9:282-90. 2007..The toxicity of EHEC-Hly to microvascular endothelial cells plausibly contributes to the virulence of the stx-negative E. coli O26 strains and to the pathogenesis of HUS... - Molecular characterization and distribution of genes encoding members of the type III effector nleA family among pathogenic Escherichia coli strainsKristina Creuzburg
Institute of Food Science and Biotechnology, Department of Food Microbiology, University of Hohenheim, 70599 Stuttgart, Germany
J Clin Microbiol 45:2498-507. 2007..Furthermore, transmission of the type III effector-encoding gene could be shown by transduction of nleA-carrying bacteriophages to a laboratory E. coli strain... - HEp-2 cell adherence, actin aggregation, and intimin types of attaching and effacing Escherichia coli strains isolated from healthy infants in Germany and AustraliaLothar Beutin
Division of Emerging Bacterial Pathogens, Department of Biological Safety, Robert Koch Institute, 13353 Berlin, Germany
Infect Immun 71:3995-4002. 2003..Our finding indicates that atypical EPEC strains could play a double role as strains that naturally immunize against intimin in humans and as reservoirs for new emerging human pathogenic EPEC strains... - Shiga toxin-producing Escherichia coli and their bacteriophages as a model for the analysis of virulence and stress response of a food-borne pathogenKristina Creuzburg
Institute of Food Science and Biotechnology, Department of Food Microbiology, University of Hohenheim, Germany
Berl Munch Tierarztl Wochenschr 120:288-95. 2007..Due to the linkage between the phage life cycle and stx expression, STEC and their bacteriophages are useful as a model for the analysis of stress response and virulence of this food-borne pathogen... - Molecular characterization of extended-spectrum beta-lactamases in Enterobacteriaceae from patients of two hospitals in Saxony, GermanyJoachim Schmitt
Institute of Medical Microbiology and Hygiene, Technical University of Dresden, Fetscherstrasse 72, Dresden, Germany
J Med Microbiol 56:241-9. 2007..The ESBL phenotypes often coincided with ciprofloxacin or gentamicin resistance... - Distribution, functional expression, and genetic organization of Cif, a phage-encoded type III-secreted effector from enteropathogenic and enterohemorrhagic Escherichia coliEstelle Loukiadis
INRA, UMR 1225, Ecole Nationale Veterinaire de Toulouse, Toulouse 31076, France
J Bacteriol 190:275-85. 2008..This diversity and the presence of nonfunctional effectors should be taken into account to assess EPEC and EHEC pathogenicity and tropism... - Serotypes and intimin types of intestinal and faecal strains of eae+ Escherichia coli from weaned pigsAnna Malik
Veterinary Medical Research Institute of the Hungarian Academy of Sciences, H-1143 Budapest, Hungaria krt. 21, Hungary
Vet Microbiol 114:82-93. 2006..Furthermore there was an indication that the PK15 cell line could be used as a model to study in vitro adherence of eae(+)E. coli of some human and porcine origin... - The Shiga toxin 1-converting bacteriophage BP-4795 encodes an NleA-like type III effector proteinKristina Creuzburg
Institute of Medical Microbiology and Hygiene, Technical University of Dresden, 01307 Dresden, Germany
J Bacteriol 187:8494-8. 2005..One of these morons encodes a type III effector that is translocated by the locus of enterocyte effacement-encoded type III secretion system into HeLa cells, where it localizes with the Golgi apparatus... - Characterization of two major groups of diarrheagenic Escherichia coli O26 strains which are globally spread in human patients and domestic animals of different speciesLuciana Leomil
Division of Microbial Toxins, Department of Biological Safety, Robert Koch Institute, D-13353 Berlin, Germany
FEMS Microbiol Lett 249:335-42. 2005..Multiple species of domestic animals were shown to serve as a reservoir for human pathogenic O26 EPEC and EHEC strains... - Genetic structure and chromosomal integration site of the cryptic prophage CP-1639 encoding Shiga toxin 1Kristina Creuzburg
, Germany
Microbiology 151:941-50. 2005..The results of this study have shown that the cryptic prophage CP-1639 is associated with an integrative element at a particular site in the E. coli chromosome that possesses high genetic variability... - Global expression of prophage genes in Escherichia coli O157:H7 strain EDL933 in response to norfloxacinSylvia Herold
, , Dresden, Germany
Antimicrob Agents Chemother 49:931-44. 2005..The results of the study have shown that a low concentration of norfloxacin has profound effects on the transcriptome of E. coli O157:H7... - Shiga toxin-encoding bacteriophages--genomes in motionSylvia Herold
, , , Fetscherstrasse 74, D-01037 Dresden, Germany
Int J Med Microbiol 294:115-21. 2004..Stx-phages may be considered to represent highly mobile genetic elements that play an important role in the expression of Stx, in horizontal gene transfer, and hence in genome diversification... - Endemic occurrence of infections by multidrug-resistant Escherichia coli of four unique serotypes in the elderly population of IsraelMoshe Wolk
Ministry of Health Central Laboratories, Yakov Eliav st 9, P O Box 34410, 91342 Jerusalem, Israel
FEMS Microbiol Lett 239:249-54. 2004..Further investigation of these strains and analysis of their virulence factors may help to confine their spread... - Transduction of porcine enteropathogenic Escherichia coli with a derivative of a shiga toxin 2-encoding bacteriophage in a porcine ligated ileal loop systemIstvan Toth
Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary
Appl Environ Microbiol 69:7242-7. 2003..These results indicate that in vivo conditions are more effective for transduction of Stx2-encoding phages than in vitro conditions... - Enteroaggregative Escherichia coli serotype O126:H27, IsraelGila Shazberg
Bikur Cholim Hospital, Jerusalem, Israel
Emerg Infect Dis 9:1170-3. 2003..We also evaluated the clinical manifestations of infection in 17 hospitalized children by our most common EAEC serotype, O126:H27... - Multilocus sequence analysis of Porphyromonas gingivalis indicates frequent recombinationAndreas Koehler
, , Germany
Microbiology 149:2407-15. 2003..gingivalis. A standardized index of association of 0.0898 indicates that the P. gingivalis genes analysed are close to linkage equilibrium... - Repression of the locus of the enterocyte effacement-encoded regulator of gene transcription of Escherichia coli O157:H7 by Lactobacillus reuteri culture supernatants is LuxS and strain dependentIvan Jelcić
Institute of Food Science and Biotechnology, Section of Food Microbiology, University of Hohenheim, Stuttgart, Germany
Appl Environ Microbiol 74:3310-4. 2008..reuteri 100-23C wild-type strain and its luxS mutant elicited a comparable effect. Furthermore, the epinephrine-mediated induction of ler expression was repressed by secreted substance(s) of L. reuteri ATCC 55730...