Herbert Schmidt

Summary

Country: Germany

Publications

  1. pmc Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
    Infect Immun 69:6863-73. 2001
  2. ncbi request reprint Shiga-toxin-converting bacteriophages
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Res Microbiol 152:687-95. 2001
  3. pmc Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage phi3538 isolated from Escherichia coli O157:H7
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, 97080 Wurzburg, Germany
    Appl Environ Microbiol 65:3855-61. 1999
  4. ncbi request reprint Antibody reactivity of a standardized human serum protein solution against a spectrum of microbial pathogens and toxins: comparison with fresh frozen plasma
    Herbert Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Würzburg and Biotest Pharma GmbH, Dreieich, Germany
    Ther Apher 6:145-53. 2002
  5. ncbi request reprint A gene cluster closely related to type II secretion pathway operons of gram-negative bacteria is located on the large plasmid of enterohemorrhagic Escherichia coli O157 strains
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    FEMS Microbiol Lett 148:265-72. 1997
  6. ncbi request reprint Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    J Infect Dis 179:115-23. 1999
  7. pmc Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universität Wüzburg, Germany
    Infect Immun 63:1055-61. 1995
  8. ncbi request reprint Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:h7
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    Microbiology 142:907-14. 1996
  9. ncbi request reprint Complete sequence of the large virulence plasmid pSFO157 of the sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- strain 3072/96
    Werner Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Josef Schneider Strasse 2, D 97070 Wurzburg, Germany
    Int J Med Microbiol 296:467-74. 2006
  10. ncbi request reprint KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 142:3305-15. 1996

Collaborators

Detail Information

Publications36

  1. pmc Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
    Infect Immun 69:6863-73. 2001
    ..We conclude that the newly described island occurs exclusively in a subgroup of STEC strains that are eae negative and contain the variant stx(2d )gene...
  2. ncbi request reprint Shiga-toxin-converting bacteriophages
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Res Microbiol 152:687-95. 2001
    ..Stx phages play an important role in the expression of Stx and in lateral gene transfer and are therefore a contribution to the emergence of new Stx-producing E. coli (STEC) variants...
  3. pmc Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage phi3538 isolated from Escherichia coli O157:H7
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, 97080 Wurzburg, Germany
    Appl Environ Microbiol 65:3855-61. 1999
    ..coli and that the lysogens produced infectious phage progeny. Stx-encoding bacteriophages are able to spread stx genes among enteric E. coli strains...
  4. ncbi request reprint Antibody reactivity of a standardized human serum protein solution against a spectrum of microbial pathogens and toxins: comparison with fresh frozen plasma
    Herbert Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Würzburg and Biotest Pharma GmbH, Dreieich, Germany
    Ther Apher 6:145-53. 2002
    ..coli. By using 3 different HSP batches in parallel, we could demonstrate antibody reactivity against important microbial pathogens and toxins. This antibody profile is essentially more homogeneous than that of 3 batches of FFP...
  5. ncbi request reprint A gene cluster closely related to type II secretion pathway operons of gram-negative bacteria is located on the large plasmid of enterohemorrhagic Escherichia coli O157 strains
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    FEMS Microbiol Lett 148:265-72. 1997
    ..coli, enteroaggregative E. coli, enterotoxigenic E. coli and enteroinvasive E. coli. The results obtained with the etp probe were confirmed by a PCR approach to specifically detect an internal fragment of the etpD gene...
  6. ncbi request reprint Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    J Infect Dis 179:115-23. 1999
    ..Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7...
  7. pmc Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universität Wüzburg, Germany
    Infect Immun 63:1055-61. 1995
    ..The EHEC hemolysin appears to have clinical importance because it occurs in all O157 strains tested and is reactive to sera of patients with hemolytic uremic syndrome...
  8. ncbi request reprint Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:h7
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    Microbiology 142:907-14. 1996
    ..It was located close to the EHEC hly operon...
  9. ncbi request reprint Complete sequence of the large virulence plasmid pSFO157 of the sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- strain 3072/96
    Werner Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Josef Schneider Strasse 2, D 97070 Wurzburg, Germany
    Int J Med Microbiol 296:467-74. 2006
    ..coli O157 virulence plasmids have a complex evolutionary origin...
  10. ncbi request reprint KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 142:3305-15. 1996
    ..coli) revealed a close association between the occurrence of EHEC-haemolysin and the katP gene in Shiga-like-toxin-producing E. coli O157 strains...
  11. ncbi request reprint Multilocus sequence analysis of Porphyromonas gingivalis indicates frequent recombination
    Andreas Koehler
    Institut für Hygiene und Mikrobiologie der Bayerischen Julius Maximilians Universität, 97080 Wurzburg, Germany
    Microbiology 149:2407-15. 2003
    ..gingivalis. A standardized index of association of 0.0898 indicates that the P. gingivalis genes analysed are close to linkage equilibrium...
  12. ncbi request reprint Antibacterials that are used as growth promoters in animal husbandry can affect the release of Shiga-toxin-2-converting bacteriophages and Shiga toxin 2 from Escherichia coli strains
    B Kohler
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 146:1085-90. 2000
    ..In vivo induction of Stx phages from lysogenic STEC may increase the amount of free phages in the intestine and therefore may contribute to the spread of STEC and development of new STEC pathotypes...
  13. pmc Enterohemolytic phenotypes and genotypes of shiga toxin-producing Escherichia coli O111 strains from patients with diarrhea and hemolytic-uremic syndrome
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    J Clin Microbiol 34:2364-7. 1996
    ....
  14. pmc Cattle can be a reservoir of sorbitol-fermenting shiga toxin-producing Escherichia coli O157:H(-) strains and a source of human diseases
    M Bielaszewska
    Institute for Medical Microbiology, The 2nd Medical Faculty, Charles University, 150 06 Prague, Czech Republic
    J Clin Microbiol 38:3470-3. 2000
    ..This is the first evidence that cattle can be a reservoir of SF STEC O157:H(-) and a source of human diseases...
  15. pmc A genomic island, termed high-pathogenicity island, is present in certain non-O157 Shiga toxin-producing Escherichia coli clonal lineages
    H Karch
    Institut für Hygiene und Mikrobiologie, D 97080 Wurzburg, 97070 Wurzburg, Germany
    Infect Immun 67:5994-6001. 1999
    ..The hypothesis that the HPI in STEC contributes to the fitness of the strains in certain ecological niches rather than to their pathogenic potential is discussed...
  16. ncbi request reprint The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 145:1005-14. 1999
    ..The EHEC-hlyA gene, however, is present in both. In conclusion, this study shows that the large plasmids of STEC are not uniform genetic elements but heterogeneous in both their gene composition and arrangement...
  17. pmc Structure and function of plasmid pColD157 of enterohemorrhagic Escherichia coli O157 and its distribution among strains from patients with diarrhea and hemolytic-uremic syndrome
    C Hofinger
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    J Clin Microbiol 36:24-9. 1998
    ..coli O157 strains isolated between 1987 and 1991 harbored plasmid pColD157, only 1 of 50 strains isolated during 1996 carried this plasmid. In addition, all strains harboring plasmid pColD157 were shown to have colicinogenic activity...
  18. pmc The nucleotide sequence of Shiga toxin (Stx) 2e-encoding phage phiP27 is not related to other Stx phage genomes, but the modular genetic structure is conserved
    Jürgen Recktenwald
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
    Infect Immun 70:1896-908. 2002
    ..Although the overall DNA sequence of phi P27 is not highly related to other known phages, the data obtained demonstrate a typical lambdoid genome structure...
  19. ncbi request reprint Heterogeneity of the prtC gene of Porphyromonas gingivalis
    M Wittstock
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Bau 17, Josef Schneider Str 2, D 97080 Wurzburg, Germany
    Oral Microbiol Immunol 15:33-9. 2000
    ..The observed heterogeneity of prtC genes may be a result of microevolution processes...
  20. pmc Molecular characterization and distribution of genes encoding members of the type III effector nleA family among pathogenic Escherichia coli strains
    Kristina Creuzburg
    Institute of Food Science and Biotechnology, Department of Food Microbiology, University of Hohenheim, 70599 Stuttgart, Germany
    J Clin Microbiol 45:2498-507. 2007
    ..Furthermore, transmission of the type III effector-encoding gene could be shown by transduction of nleA-carrying bacteriophages to a laboratory E. coli strain...
  21. pmc Genetic analysis of enteropathogenic and enterohemorrhagic Escherichia coli serogroup O103 strains by molecular typing of virulence and housekeeping genes and pulsed-field gel electrophoresis
    Lothar Beutin
    Division of Microbial Toxins, Department of Biological Safety, Robert Koch Institute, Berlin, Germany
    J Clin Microbiol 43:1552-63. 2005
    ..Novel virulence markers, such as efa (EHEC factor for adherence), paa (porcine adherence factor), and cif (cell cycle-inhibiting factor), were found widely associated with E. coli O103 EPEC and STEC strains...
  22. pmc HEp-2 cell adherence, actin aggregation, and intimin types of attaching and effacing Escherichia coli strains isolated from healthy infants in Germany and Australia
    Lothar Beutin
    Division of Emerging Bacterial Pathogens, Department of Biological Safety, Robert Koch Institute, 13353 Berlin, Germany
    Infect Immun 71:3995-4002. 2003
    ..Our finding indicates that atypical EPEC strains could play a double role as strains that naturally immunize against intimin in humans and as reservoirs for new emerging human pathogenic EPEC strains...
  23. ncbi request reprint Hemolysin from Shiga toxin-negative Escherichia coli O26 strains injures microvascular endothelium
    Thomas Aldick
    Institute of Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University of Munster, Robert Koch Str 41, 48149 Munster, Germany
    Microbes Infect 9:282-90. 2007
    ..The toxicity of EHEC-Hly to microvascular endothelial cells plausibly contributes to the virulence of the stx-negative E. coli O26 strains and to the pathogenesis of HUS...
  24. ncbi request reprint Molecular characterization of extended-spectrum beta-lactamases in Enterobacteriaceae from patients of two hospitals in Saxony, Germany
    Joachim Schmitt
    Institute of Medical Microbiology and Hygiene, Technical University of Dresden, Fetscherstrasse 72, Dresden, Germany
    J Med Microbiol 56:241-9. 2007
    ..The ESBL phenotypes often coincided with ciprofloxacin or gentamicin resistance...
  25. ncbi request reprint Shiga toxin-producing Escherichia coli and their bacteriophages as a model for the analysis of virulence and stress response of a food-borne pathogen
    Kristina Creuzburg
    Institute of Food Science and Biotechnology, Department of Food Microbiology, University of Hohenheim, Germany
    Berl Munch Tierarztl Wochenschr 120:288-95. 2007
    ..Due to the linkage between the phage life cycle and stx expression, STEC and their bacteriophages are useful as a model for the analysis of stress response and virulence of this food-borne pathogen...
  26. pmc Distribution, functional expression, and genetic organization of Cif, a phage-encoded type III-secreted effector from enteropathogenic and enterohemorrhagic Escherichia coli
    Estelle Loukiadis
    INRA, UMR 1225, Ecole Nationale Veterinaire de Toulouse, Toulouse 31076, France
    J Bacteriol 190:275-85. 2008
    ..This diversity and the presence of nonfunctional effectors should be taken into account to assess EPEC and EHEC pathogenicity and tropism...
  27. ncbi request reprint Serotypes and intimin types of intestinal and faecal strains of eae+ Escherichia coli from weaned pigs
    Anna Malik
    Veterinary Medical Research Institute of the Hungarian Academy of Sciences, H 1143 Budapest, Hungaria krt 21, Hungary
    Vet Microbiol 114:82-93. 2006
    ..Furthermore there was an indication that the PK15 cell line could be used as a model to study in vitro adherence of eae(+)E. coli of some human and porcine origin...
  28. pmc The Shiga toxin 1-converting bacteriophage BP-4795 encodes an NleA-like type III effector protein
    Kristina Creuzburg
    Institute of Medical Microbiology and Hygiene, Technical University of Dresden, 01307 Dresden, Germany
    J Bacteriol 187:8494-8. 2005
    ..One of these morons encodes a type III effector that is translocated by the locus of enterocyte effacement-encoded type III secretion system into HeLa cells, where it localizes with the Golgi apparatus...
  29. ncbi request reprint Characterization of two major groups of diarrheagenic Escherichia coli O26 strains which are globally spread in human patients and domestic animals of different species
    Luciana Leomil
    Division of Microbial Toxins, Department of Biological Safety, Robert Koch Institute, D 13353 Berlin, Germany
    FEMS Microbiol Lett 249:335-42. 2005
    ..Multiple species of domestic animals were shown to serve as a reservoir for human pathogenic O26 EPEC and EHEC strains...
  30. ncbi request reprint Genetic structure and chromosomal integration site of the cryptic prophage CP-1639 encoding Shiga toxin 1
    Kristina Creuzburg
    Institut für Medizinische Mikrobiologie und Hygiene der TU Dresden, Germany
    Microbiology 151:941-50. 2005
    ..The results of this study have shown that the cryptic prophage CP-1639 is associated with an integrative element at a particular site in the E. coli chromosome that possesses high genetic variability...
  31. pmc Global expression of prophage genes in Escherichia coli O157:H7 strain EDL933 in response to norfloxacin
    Sylvia Herold
    Institut für Medizinishe Mikrobioloie und Hygiene, Technische Universitat Dresden, Dresden, Germany
    Antimicrob Agents Chemother 49:931-44. 2005
    ..The results of the study have shown that a low concentration of norfloxacin has profound effects on the transcriptome of E. coli O157:H7...
  32. ncbi request reprint Shiga toxin-encoding bacteriophages--genomes in motion
    Sylvia Herold
    Institut fur Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät Carl Gustav Carus, Technische Universitat Dresden, Fetscherstrasse 74, D 01037 Dresden, Germany
    Int J Med Microbiol 294:115-21. 2004
    ..Stx-phages may be considered to represent highly mobile genetic elements that play an important role in the expression of Stx, in horizontal gene transfer, and hence in genome diversification...
  33. ncbi request reprint Endemic occurrence of infections by multidrug-resistant Escherichia coli of four unique serotypes in the elderly population of Israel
    Moshe Wolk
    Ministry of Health Central Laboratories, Yakov Eliav st 9, P O Box 34410, 91342 Jerusalem, Israel
    FEMS Microbiol Lett 239:249-54. 2004
    ..Further investigation of these strains and analysis of their virulence factors may help to confine their spread...
  34. pmc Transduction of porcine enteropathogenic Escherichia coli with a derivative of a shiga toxin 2-encoding bacteriophage in a porcine ligated ileal loop system
    Istvan Toth
    Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary
    Appl Environ Microbiol 69:7242-7. 2003
    ..These results indicate that in vivo conditions are more effective for transduction of Stx2-encoding phages than in vitro conditions...
  35. pmc Enteroaggregative Escherichia coli serotype O126:H27, Israel
    Gila Shazberg
    Bikur Cholim Hospital, Jerusalem, Israel
    Emerg Infect Dis 9:1170-3. 2003
    ..We also evaluated the clinical manifestations of infection in 17 hospitalized children by our most common EAEC serotype, O126:H27...
  36. pmc Repression of the locus of the enterocyte effacement-encoded regulator of gene transcription of Escherichia coli O157:H7 by Lactobacillus reuteri culture supernatants is LuxS and strain dependent
    Ivan Jelcić
    Institute of Food Science and Biotechnology, Section of Food Microbiology, University of Hohenheim, Stuttgart, Germany
    Appl Environ Microbiol 74:3310-4. 2008
    ..reuteri 100-23C wild-type strain and its luxS mutant elicited a comparable effect. Furthermore, the epinephrine-mediated induction of ler expression was repressed by secreted substance(s) of L. reuteri ATCC 55730...