Gereon Schares

Summary

Country: Germany

Publications

  1. doi request reprint Novel tools for the diagnosis and differentiation of acute and chronic bovine besnoitiosis
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Int J Parasitol 43:143-54. 2013
  2. ncbi request reprint Oocysts of Neospora caninum, Hammondia heydorni, Toxoplasma gondii and Hammondia hammondi in faeces collected from dogs in Germany
    G Schares
    Institute of Epidemiology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Seestrasse 55, 16868 Wusterhausen, Germany
    Int J Parasitol 35:1525-37. 2005
  3. doi request reprint Occurrence of Toxoplasma gondii and Hammondia hammondi oocysts in the faeces of cats from Germany and other European countries
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, D 16868 Wusterhausen, Germany
    Vet Parasitol 152:34-45. 2008
  4. doi request reprint Characterization of a repetitive DNA fragment in Hammondia hammondi and its utility for the specific differentiation of H. hammondi from Toxoplasma gondii by PCR
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, Wusterhausen, Germany
    Mol Cell Probes 22:244-51. 2008
  5. ncbi request reprint Neospora caninum in dairy herds in Schleswig-Holstein, Germany
    Gereon Schares
    Friedrich Loeffler Institut Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Berl Munch Tierarztl Wochenschr 122:47-50. 2009
  6. doi request reprint First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, D 16868 Wusterhausen, Germany
    Vet Parasitol 163:315-22. 2009
  7. doi request reprint Comparative evaluation of immunofluorescent antibody and new immunoblot tests for the specific detection of antibodies against Besnoitia besnoiti tachyzoites and bradyzoites in bovine sera
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, D 16868 Wusterhausen, Germany
    Vet Parasitol 171:32-40. 2010
  8. doi request reprint Evaluation of a commercial ELISA for the specific detection of antibodies against Besnoitia besnoiti
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Vet Parasitol 175:52-9. 2011
  9. doi request reprint Quantitative real time polymerase chain reaction assays for the sensitive detection of Besnoitia besnoiti infection in cattle
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Vet Parasitol 178:208-16. 2011
  10. pmc Peptide microarray analysis of in silico-predicted epitopes for serological diagnosis of Toxoplasma gondii infection in humans
    Pavlo Maksimov
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Clin Vaccine Immunol 19:865-74. 2012

Collaborators

Detail Information

Publications52

  1. doi request reprint Novel tools for the diagnosis and differentiation of acute and chronic bovine besnoitiosis
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Int J Parasitol 43:143-54. 2013
    ..The detection of parasite DNA in skin by real-time PCR was clearly superior to serological analysis in detecting infected cattle during acute besnoitiosis...
  2. ncbi request reprint Oocysts of Neospora caninum, Hammondia heydorni, Toxoplasma gondii and Hammondia hammondi in faeces collected from dogs in Germany
    G Schares
    Institute of Epidemiology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Seestrasse 55, 16868 Wusterhausen, Germany
    Int J Parasitol 35:1525-37. 2005
    ..06. It may thus be possible to develop criteria for a preliminary identification of N. caninum in dog faeces based on the oocyst morphology...
  3. doi request reprint Occurrence of Toxoplasma gondii and Hammondia hammondi oocysts in the faeces of cats from Germany and other European countries
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, D 16868 Wusterhausen, Germany
    Vet Parasitol 152:34-45. 2008
    ..A PCR-RFLP analysis for the SAG2, SAG3, GRA6 and BTUB genes revealed T. gondii genotype II in all cases. Morphologically, H. hammondi oocysts exhibited a statistically significantly smaller Length-Width-Ratio than T. gondii oocysts...
  4. doi request reprint Characterization of a repetitive DNA fragment in Hammondia hammondi and its utility for the specific differentiation of H. hammondi from Toxoplasma gondii by PCR
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, Wusterhausen, Germany
    Mol Cell Probes 22:244-51. 2008
    ..hammondi provides a foundation for the design of primers specific to T. gondii, and the future optimisation of conventional and real-time PCR assays for the specific diagnosis of toxoplasmosis in definitive and intermediate hosts...
  5. ncbi request reprint Neospora caninum in dairy herds in Schleswig-Holstein, Germany
    Gereon Schares
    Friedrich Loeffler Institut Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Berl Munch Tierarztl Wochenschr 122:47-50. 2009
    ..Similar to the results obtained in Rhineland-Palatinate, the observation of positive herds in the present study was associated with an elevated population density...
  6. doi request reprint First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, D 16868 Wusterhausen, Germany
    Vet Parasitol 163:315-22. 2009
    ..besnoiti isolates. A potential association between growth properties and differences in virulence remains to be established. This is the first in vitro isolation of B. besnoiti from cattle in Germany...
  7. doi request reprint Comparative evaluation of immunofluorescent antibody and new immunoblot tests for the specific detection of antibodies against Besnoitia besnoiti tachyzoites and bradyzoites in bovine sera
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, D 16868 Wusterhausen, Germany
    Vet Parasitol 171:32-40. 2010
    ..besnoiti antigens which may help to develop new specific and sensitive serological tests based on individual antigens and in the identification of possible vaccine candidates...
  8. doi request reprint Evaluation of a commercial ELISA for the specific detection of antibodies against Besnoitia besnoiti
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Vet Parasitol 175:52-9. 2011
    ..Thus it may be used to support control measures, e.g., for the separation of infected animals from the remaining herd to avoid a further transmission of the infection within the herd...
  9. doi request reprint Quantitative real time polymerase chain reaction assays for the sensitive detection of Besnoitia besnoiti infection in cattle
    G Schares
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Vet Parasitol 178:208-16. 2011
    ..besnoiti real time PCR assay (BbRT2) was specific for B. besnoiti, B. bennetti and B. tarandi, but did not react when 10 ng DNA of other related parasite species from the genus Besnoitia or other genera were subjected to analysis...
  10. pmc Peptide microarray analysis of in silico-predicted epitopes for serological diagnosis of Toxoplasma gondii infection in humans
    Pavlo Maksimov
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Clin Vaccine Immunol 19:865-74. 2012
    ..gondii epitopes as candidate antigens for serological diagnosis...
  11. pmc Toxoplasma gondii sexual cross in a single naturally infected feline host: generation of highly mouse-virulent and avirulent clones, genotypically different from clonal types I, II and III
    Daland C Herrmann
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestr, 55, 16868, Wusterhausen, Germany
    Vet Res 43:39. 2012
    ..gondii occur under natural conditions and result in the emergence of clones with increased virulence in mice...
  12. ncbi request reprint Comparison of different commercial DNA extraction kits to detect Toxoplasma gondii oocysts in cat faeces
    Daland C Herrmann
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Berl Munch Tierarztl Wochenschr 124:497-502. 2011
    ..These two kits revealed a similar, even slightly lower detection limit (50 oocysts per 1 g of sample) when tested with faecal samples spiked with T gondii oocysts...
  13. ncbi request reprint p38-avidity-ELISA: examination of herds experiencing epidemic or endemic Neospora caninum-associated bovine abortion
    G Schares
    Federal Research Centre for Virus Diseases of Animals, Institute for Epidemiological Diagnostics and Institute for Epidemiology, Seestrasse 55, D 16868, Wusterhausen, Germany
    Vet Parasitol 106:293-305. 2002
    ..e. the severity of the abortion epidemic). A possible explanation for this observation is that herds experiencing heavy abortion are sampled earlier after the onset of the epidemic than others that have a less severe abortion storm...
  14. ncbi request reprint Regional distribution of bovine Neospora caninum infection in the German state of Rhineland-Palatinate modelled by Logistic regression
    G Schares
    Institute of Epidemiology, Federal Research Centre for Virus Diseases of Animals, Seestrasse 55, D 16868 Wusterhausen, Germany
    Int J Parasitol 33:1631-40. 2003
    ....
  15. ncbi request reprint Use of purified tachyzoite surface antigen p38 in an ELISA to diagnose bovine neosporosis
    G Schares
    Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Seestrasse 55, D 16868, Wusterhausen, Germany
    Int J Parasitol 30:1123-30. 2000
    ..It may thus be appropriate to apply different cut-offs in serological tests depending on the abortion pattern to be analysed...
  16. doi request reprint Development of a multiplex real time PCR to differentiate Sarcocystis spp. affecting cattle
    Gastón Moré
    Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, 16868 Wusterhausen, Germany
    Vet Parasitol 197:85-94. 2013
    ..5% and 41.7%, 36.3% and 95.9% respectively. Improved DNA extraction methods may allow to further increase the specific and sensitive detection of Sarcocystis spp. in meat samples...
  17. doi request reprint Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany
    Gastón Moré
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Südufer 10, 17493 Greifswald Insel Riems, Germany
    Parasitology 141:646-51. 2014
    ..Our research shows that information on the infection of snakes with Sarcocystis spp. may be used to assess compliance with regulations on the trade with wildlife species...
  18. ncbi request reprint Bovine neosporosis: comparison of serological methods using outbreak sera from a dairy herd in New Zealand
    G Schares
    Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Wusterhausen, Germany
    Int J Parasitol 29:1659-67. 1999
    ..Discrepancies in positive-negative classification between the two commercial ELISAs and one of the in-house ELISAs were due mainly to differences in the cut-off selection...
  19. ncbi request reprint Potential risk factors for bovine Neospora caninum infection in Germany are not under the control of the farmers
    G Schares
    Institute of Epidemiology, Federal Research Centre for Virus Diseases of Animals, Seestrasse 55, 16868 Wusterhausen, Germany
    Parasitology 129:301-9. 2004
    ..In addition, it suggests that the risk a herd has to acquire N. caninum infections is also associated with factors related to the farm location, i.e. factors that are largely out of the control of farmers...
  20. ncbi request reprint Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines
    D von Blumröder
    Federal Research Centre for Virus Diseases of Animals, Institute of Epidemiology, Seestrasse 55, D 16868 Wusterhausen, Germany
    Vet Parasitol 120:11-22. 2004
    ..This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies...
  21. ncbi request reprint Cyclic transmission of Neospora caninum: serological findings in dogs shedding oocysts
    G Schares
    Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Wusterhausen, Germany
    Parasitol Res 87:873-7. 2001
    ..Therefore, our study provides initial information on a 152 kDa tachyzoite antigen, which might be a suitable candidate to identify dogs with a history of shedding N. caninum oocysts...
  22. ncbi request reprint In contrast to dogs, red foxes (Vulpes vulpes) did not shed neospora caninum upon feeding of intermediate host tissues
    Gereon Schares
    Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Wusterhausen, Germany
    Parasitol Res 88:44-52. 2002
    ..like oocysts collected from fox faeces. These results suggest that the red fox is either an inappropriate final host for N. caninum or not at all a final host for this parasite...
  23. doi request reprint Atypical Toxoplasma gondii genotypes identified in oocysts shed by cats in Germany
    D C Herrmann
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, 16868 Wusterhausen, Germany
    Int J Parasitol 40:285-92. 2010
    ..To our knowledge this is the first description of the presence of T. gondii genotypes different from the clonal Types I, II and III in the faeces of naturally infected cats...
  24. doi request reprint Molecular comparison of Neospora caninum oocyst isolates from naturally infected dogs with cell culture-derived tachyzoites of the same isolates using nested polymerase chain reaction to amplify microsatellite markers
    W Basso
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, 16868 Wusterhausen, Germany
    Vet Parasitol 160:43-50. 2009
    ..Our technique may allow the typing of clinical samples and different strains of N. caninum at the molecular level. This method may prove useful for the identification of infection sources in molecular epidemiological studies...
  25. ncbi request reprint Hammondia heydorni-like oocysts shed by a naturally infected dog and Neospora caninum NC-1 cannot be distinguished
    G Schares
    Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Wusterhausen, Germany
    Parasitol Res 87:808-16. 2001
    ..caninum or other protozoan parasites that use dogs as final hosts and have an oocyst morphology resembling that of I. bigemina/I. heydorni/H. heydorni...
  26. ncbi request reprint The efficiency of vertical transmission of Neospora caninum in dairy cattle analysed by serological techniques
    G Schares
    Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Wusterhausen, Germany
    Vet Parasitol 80:87-98. 1998
    ..The results of this study suggest that the N. caninum-infection can be maintained over several generations at a nearly constant prevalence level, apparently without a need for dispersion by an definitive host...
  27. ncbi request reprint Adaptation of a surface antigen-based ELISA for the detection of antibodies against Neospora caninum in bovine milk
    G Schares
    Institute of Epidemiology, Friedrich Loeffler Institut Federal Research Institute for Animal Health, Seestrasse 55, D 16868 Wusterhausen, Germany
    J Vet Med B Infect Dis Vet Public Health 52:45-8. 2005
    ..The optimized linear regression model had an adjusted R(2) of 0.8501...
  28. ncbi request reprint Adaptation of a commercial ELISA for the detection of antibodies against Neospora caninum in bovine milk
    G Schares
    Institute of Epidemiology, Federal Research Center for Virus, Diseases of Animals, Seestrasse 55, D 16868 Wusterhausen, Germany
    Vet Parasitol 120:55-63. 2004
    ..The milk-based commercial ELISA classified more aborting dams as positive than the serum-based ELISA with this cut-off. The milk ELISA may be a valuable tool to assess the herd status with regard to abortion caused by N. caninum...
  29. pmc Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany
    Pavlo Maksimov
    Institute of Epidemiology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    PLoS ONE 8:e80213. 2013
    ..The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of T. gondii and to determine by serotyping the T. gondii clonal types prevailing in cats as intermediate hosts in Germany...
  30. ncbi request reprint The "Friedrich-Loeffler-Institut": past, present and future of research in infectious diseases of animals
    Franz J Conraths
    Friedrich Loeffler Institut, Institut fur Epidemiologie, Bundesforschungsinstitut für Tiergesundheit, Wusterhausen, Germany
    Berl Munch Tierarztl Wochenschr 118:354-64. 2005
    ....
  31. ncbi request reprint Neospora caninum and Waddlia chondrophila strain 2032/99 in a septic stillborn calf
    K Henning
    Federal Research Centre of Virus Diseases of Animals, Seestrasse 55, D 16868, Wusterhausen, Germany
    Vet Microbiol 85:285-92. 2002
    ..chondrophila or a closely related organism...
  32. doi request reprint First isolation of Neospora caninum from the faeces of a dog from Portugal
    W Basso
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, 16868 Wusterhausen, Germany
    Vet Parasitol 159:162-6. 2009
    ..The DNA-microsatellite pattern observed in the NC-P1 isolate was not previously reported for any N. caninum isolate. To our knowledge, this is the first isolation of N. caninum from the faeces of a naturally infected dog from Portugal...
  33. ncbi request reprint Validation of molecular-diagnostic techniques in the parasitological laboratory
    Franz J Conraths
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestr 55, D 16868 Wusterhausen, Germany
    Vet Parasitol 136:91-8. 2006
    ..After an initial validation, test performance needs to be continuously monitored, e.g. by using combined Shewhart-CUSUM control routines, and test results compared to those obtained by other laboratories (proficiency testing)...
  34. ncbi request reprint Serological evidence for naturally occurring transmission of Neospora caninum among foxes (Vulpes vulpes)
    G Schares
    Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Seestrasse 55, D 16868 Wusterhausen, Germany
    Int J Parasitol 31:418-23. 2001
    ..This result provides the first evidence for the postnatal or vertical transmission of N. caninum among naturally seropositive foxes...
  35. pmc Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray
    Pavlo Maksimov
    Friedrich Loeffler Institut, Wusterhausen, Germany
    PLoS ONE 7:e34212. 2012
    ..Serotyping is a novel technique which may allow to determine the clonal type of T. gondii humans are infected with and to extend typing studies to larger populations which include infected but non-diseased individuals...
  36. ncbi request reprint Neospora caninum: identification of 19-, 38-, and 40-kDa surface antigens and a 33-kDa dense granule antigen using monoclonal antibodies
    G Schares
    Institut für epidemiologische Diagnostik, Bundesforschungsanstalt fur Viruskrankheiten der Tiere, Seestrasse 55, Wusterhausen, D 16868, Germany
    Exp Parasitol 92:109-19. 1999
    ..Immunohistochemical analysis of infected tissue sections indicated that the 33-kDa dense granule antigen is present in both tachyzoites and bradyzoites, while the 38-kDa surface antigen from tachyzoites seems to be absent in bradyzoites...
  37. ncbi request reprint A Hammondia-like parasite from the European fox (Vulpes vulpes) forms biologically viable tissue cysts in cell culture
    G Schares
    Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Seestrasse 55, D 16868, Wusterhausen, Germany
    Int J Parasitol 33:229-34. 2003
    ..Since in vitro cystogenesis of dog-derived Hammondia heydorni has not been observed yet, in vitro cyst formation might be one trait to separate fox-derived Hammondia sp. from H. heydorni on a species level...
  38. ncbi request reprint Detection of specific antibodies to Neospora caninum and Toxoplasma gondii in naturally infected alpacas (Lama pacos), llamas (Lama glama) and vicunas (Lama vicugna) from Peru and Germany
    D Wolf
    Institute of Parasitology, Justus Liebig University Giessen, Rudolf Buchheim Strasse 2, D 35392 Giessen, Germany
    Vet Parasitol 130:81-7. 2005
    ..However, our results confirm natural infections with N. caninum and T. gondii in SAC. Whether these infections are linked to any disease, e.g. reproductive losses, has to be clarified in further studies...
  39. ncbi request reprint Hammondia isolated from dogs and foxes are genetically distinct
    J Abel
    Department of Cell and Molecular Biology, University of Technology, Sydney, Westbourne Street, Gore Hill, NSW 2065, Australia
    Parasitology 132:187-92. 2006
    ..This study therefore provides evidence that H. heydorni oocysts from dogs and foxes represent 2 distinct genetic lineages that can be differentiated using a PCR, which targets the alpha tubulin locus...
  40. ncbi request reprint Supranational comparison of Neospora caninum seroprevalences in cattle in Germany, The Netherlands, Spain and Sweden
    C J M Bartels
    Animal Health Service Ltd, P O Box 9, 7400 AA Deventer, The Netherlands
    Vet Parasitol 137:17-27. 2006
    ..caninum infection varied greatly within in Europe. Estimates of prevalence can be used to calculate the economic impact of N. caninum infection as well as to evaluate the effect of prevention and control strategies over time...
  41. ncbi request reprint Incidence of Neospora caninum and other intestinal protozoan parasites in populations of Swiss dogs
    H Sager
    Institute of Parasitology, Vetsuisse Faculty, University of Bern, CH 3001 Bern, Switzerland
    Vet Parasitol 139:84-92. 2006
    ..All gerbils were seronegative for N. caninum at 5 weeks p.i. A N. caninum-seroprevalence of 7.8% was determined by ELISA upon 1132 serum samples collected from dogs randomly selected by veterinarians among their clinical patients...
  42. ncbi request reprint Diagnosis of bovine neosporosis
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agricultural, Beltsville, MD 20705, USA
    Vet Parasitol 140:1-34. 2006
    ..Although not a routine method of diagnosis, methods to isolate viable N. caninum from bovine tissues are also reviewed...
  43. ncbi request reprint Bovine neosporosis: immunoblot improves foetal serology
    P Söndgen
    Staatliches Veterinäruntersuchungsamt, Zur Taubeneiche 10 12, D 59821, Arnsberg, Germany
    Vet Parasitol 102:279-90. 2001
    ..However, also the specificity of the IB was superior to that of the IFAT in relation to histology/immunohistochemistry, maternal serology and PCR...
  44. ncbi request reprint Neospora caninum IgG avidity tests: an interlaboratory comparison
    C Bjorkman
    Department of Clinical Sciences, Swedish University of Agricultural Sciences, SE 750 07 Uppsala, Sweden
    Vet Parasitol 140:273-80. 2006
    ..Our experience suggests that avidity tests are sometimes less robust than conventional ELISAs. Therefore, it is essential that they are carefully standardised and their performance continuously evaluated...
  45. ncbi request reprint Evaluation of four serological techniques to determine the seroprevalence of Neospora caninum in foxes (Vulpes vulpes) and coyotes (Canis latrans) on Prince Edward Island, Canada
    W Wapenaar
    Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, C1A 4P3, PEI, Canada
    Vet Parasitol 145:51-8. 2007
    ..Although the seroprevalence observed was low, N. caninum antibodies are present in foxes and coyotes on Prince Edward Island (PEI) and their role in the N. caninum epidemiology needs further study...
  46. ncbi request reprint An interlaboratory comparison of immunohistochemistry and PCR methods for detection of Neospora caninum in bovine foetal tissues
    C van Maanen
    Animal Health Service, P O Box 9, NL 7400 AA Deventer, The Netherlands
    Vet Parasitol 126:351-64. 2004
    ..e. single or nested) and diagnostic sensitivity. Consequently, an improvement of diagnostic performance of PCR might possibly be achieved by optimizing DNA extraction methods...
  47. pmc Epidemiology and control of neosporosis and Neospora caninum
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U S Department of Agriculture, Beltsville, MD 20705, USA
    Clin Microbiol Rev 20:323-67. 2007
    ..caninum in animals and humans are tabulated. The role of wildlife in the life cycle of N. caninum and strategies for the control of neosporosis in cattle are discussed...
  48. ncbi request reprint Neospora caninum infection in Greek dairy cattle herds detected by two antibody assays in individual milk samples
    S Sotiraki
    Veterinary Research Institute, National Agricultural Research Foundation, Ionia, 57008 Thessaloniki, Greece
    Vet Parasitol 152:79-84. 2008
    ..In conclusion, N. caninum infection is present among dairy cattle in Greece, deserving more studies to determine its contribution to abortion problems in Greek dairy herds...
  49. ncbi request reprint HPLC purification of recombinant NcGRA6 antigen improves enzyme-linked immunosorbent assay for serodiagnosis of bovine neosporosis
    M C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Vet Parasitol 131:227-34. 2005
    ..coli proteins improves the performance of recombinant NcGRA6 ELISA in diagnosing bovine neosporosis, and may have applicability to the use of recombinant proteins in diagnosing other infectious agents...
  50. ncbi request reprint Cross-sectional survey in pig breeding farms in Hesse, Germany: seroprevalence and risk factors of infections with Toxoplasma gondii, Sarcocystis spp. and Neospora caninum in sows
    I M Damriyasa
    Institute of Parasitology, Justus Liebig University Giessen, Rudolf Buchheim Strasse 2, 35392 Giessen, Germany
    Vet Parasitol 126:271-86. 2004
    ..Antibodies to N. caninum were detected in one sow using both the screening ELISA and the confirmatory immunoblotting technique. This may indicate the first natural N. caninum infection in pigs...
  51. ncbi request reprint [Zoonoses in working- and wild animals and their significance in Germany. An overview]
    F J Conraths
    Bundesforschungsanstalt fur Viruskrankheiten der Tiere, Wusterhausen
    Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 47:633-46. 2004
    ..Against this background, the current knowledge on important zoonoses transmitted from livestock and some wildlife animals to humans as well as the epidemiological situation in Germany with regard to these diseases is summarized...
  52. pmc Neospora caninum microneme protein NcMIC3: secretion, subcellular localization, and functional involvement in host cell interaction
    A Naguleswaran
    Institute of Parasitology, University of Berne, CH-3012 Bern, Switzerland
    Infect Immun 69:6483-94. 2001
    ..Our data are indicative of a functional role of NcMIC3 in host cell infection...