P Saftig

Summary

Country: Germany

Publications

  1. pmc Impaired osteoclastic bone resorption leads to osteopetrosis in cathepsin-K-deficient mice
    P Saftig
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, Gosslerstrasse 12 d, 37073 Gottingen, Germany
    Proc Natl Acad Sci U S A 95:13453-8. 1998
  2. ncbi request reprint Functions of cathepsin K in bone resorption. Lessons from cathepsin K deficient mice
    P Saftig
    Abteilung Biochemie II, Universitat Gottingen, Germany
    Adv Exp Med Biol 477:293-303. 2000
  3. ncbi request reprint Overlapping functions of lysosomal acid phosphatase (LAP) and tartrate-resistant acid phosphatase (Acp5) revealed by doubly deficient mice
    A Suter
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Biochemie II, Universitat Gottingen, Heinrich Düker Weg 12, 37073 Gottingen, Germany
    Development 128:4899-910. 2001
  4. ncbi request reprint Normal lysosomal morphology and function in LAMP-1-deficient mice
    N Andrejewski
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, 37073 Gottingen, Germany
    J Biol Chem 274:12692-701. 1999
  5. ncbi request reprint Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice
    Y Tanaka
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Biochemie II, Universitat Gottingen, Germany
    Nature 406:902-6. 2000
  6. ncbi request reprint Disease model: LAMP-2 enlightens Danon disease
    P Saftig
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Biochemie II, Universitat Gottingen, Heinrich Düker Weg 12, 37073 Gottingen, Germany
    Trends Mol Med 7:37-9. 2001
  7. ncbi request reprint Mice deficient in lysosomal acid phosphatase develop lysosomal storage in the kidney and central nervous system
    P Saftig
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, Gosslerstrasse 12d, 37073 Gottingen, Federal Republic of Germany
    J Biol Chem 272:18628-35. 1997
  8. pmc Targeted disruption of the arylsulfatase B gene results in mice resembling the phenotype of mucopolysaccharidosis VI
    M Evers
    Abteilung Biochemie II, Universitat Gottingen, Germany
    Proc Natl Acad Sci U S A 93:8214-9. 1996
  9. ncbi request reprint Spermatogenesis proceeds normally in mice without linker histone H1t
    B Drabent
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Molekularbiologie, Universitat Gottingen, Germany
    Histochem Cell Biol 113:433-42. 2000
  10. ncbi request reprint Amyloidogenic processing of human amyloid precursor protein in hippocampal neurons devoid of cathepsin D
    P Saftig
    Zentrum Biochemie und Molekular Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, Gosslerstrasse 12d, 37073 Gottingen
    J Biol Chem 271:27241-4. 1996

Collaborators

Detail Information

Publications33

  1. pmc Impaired osteoclastic bone resorption leads to osteopetrosis in cathepsin-K-deficient mice
    P Saftig
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, Gosslerstrasse 12 d, 37073 Gottingen, Germany
    Proc Natl Acad Sci U S A 95:13453-8. 1998
    ..Assaying the resorptive activity of cathepsin-K-deficient osteoclasts in vitro revealed this function to be severely impaired, which supports the contention that cathepsin K is of major importance in bone remodeling...
  2. ncbi request reprint Functions of cathepsin K in bone resorption. Lessons from cathepsin K deficient mice
    P Saftig
    Abteilung Biochemie II, Universitat Gottingen, Germany
    Adv Exp Med Biol 477:293-303. 2000
    ..Taken together the phenotype of cathepsin K knockout mice underlines the importance of this proteinase in bone remodelling...
  3. ncbi request reprint Overlapping functions of lysosomal acid phosphatase (LAP) and tartrate-resistant acid phosphatase (Acp5) revealed by doubly deficient mice
    A Suter
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Biochemie II, Universitat Gottingen, Heinrich Düker Weg 12, 37073 Gottingen, Germany
    Development 128:4899-910. 2001
    ..We conclude that for several substrates LAP and Acp5 can substitute for each other and that these acid phosphatases are essential for processing of non-collagenous proteins, including osteopontin, by osteoclasts...
  4. ncbi request reprint Normal lysosomal morphology and function in LAMP-1-deficient mice
    N Andrejewski
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, 37073 Gottingen, Germany
    J Biol Chem 274:12692-701. 1999
    ..The increase of LAMP-2 was neither correlated with an increase in the level of lamp-2 mRNAs nor with increased half-life time of LAMP-2. This findings suggest a translational regulation of LAMP-2 expression...
  5. ncbi request reprint Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice
    Y Tanaka
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Biochemie II, Universitat Gottingen, Germany
    Nature 406:902-6. 2000
    ..This theory is further substantiated by the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes...
  6. ncbi request reprint Disease model: LAMP-2 enlightens Danon disease
    P Saftig
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Biochemie II, Universitat Gottingen, Heinrich Düker Weg 12, 37073 Gottingen, Germany
    Trends Mol Med 7:37-9. 2001
    ..LAMP-2 deficient mice represent a valuable animal model of Danon disease. They will further be used to study the exact role of LAMP-2 in autophagy and to analyse the consequences of an impaired autophagic pathway in various tissues...
  7. ncbi request reprint Mice deficient in lysosomal acid phosphatase develop lysosomal storage in the kidney and central nervous system
    P Saftig
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, Gosslerstrasse 12d, 37073 Gottingen, Federal Republic of Germany
    J Biol Chem 272:18628-35. 1997
    ..The causal relationship of the enzyme defect to the clinical manifestations remains to be determined...
  8. pmc Targeted disruption of the arylsulfatase B gene results in mice resembling the phenotype of mucopolysaccharidosis VI
    M Evers
    Abteilung Biochemie II, Universitat Gottingen, Germany
    Proc Natl Acad Sci U S A 93:8214-9. 1996
    ..This mouse model will be a valuable tool for studying pathogenesis of MPS VI and may help to evaluate therapeutical approaches for lysosomal storage diseases...
  9. ncbi request reprint Spermatogenesis proceeds normally in mice without linker histone H1t
    B Drabent
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Molekularbiologie, Universitat Gottingen, Germany
    Histochem Cell Biol 113:433-42. 2000
    ..RNase protection analysis demonstrated that H1.1, H1.2 and H1.4 histone gene expression is enhanced during spermatogenesis in H1t-deficient mice...
  10. ncbi request reprint Amyloidogenic processing of human amyloid precursor protein in hippocampal neurons devoid of cathepsin D
    P Saftig
    Zentrum Biochemie und Molekular Zellbiologie, Abteilung Biochemie II, Universitat Gottingen, Gosslerstrasse 12d, 37073 Gottingen
    J Biol Chem 271:27241-4. 1996
    ..The results rule out cathepsin D as a critical component of alpha-, beta-, or gamma-secretase and therefore as a primary target for drugs aimed at decreasing the betaA4-amyloid peptide burden in Alzheimer's disease...
  11. pmc mu1A-adaptin-deficient mice: lethality, loss of AP-1 binding and rerouting of mannose 6-phosphate receptors
    C Meyer
    Zentrum für Biochemie und Molekulare Zellbiologie, Dept Biochemie II, Universitat Gottingen, Heinrich Düker Weg 12, D 37073 Gottingen, Germany
    EMBO J 19:2193-203. 2000
    ..MPR46 fails to recycle back from the endosome to the TGN, indicating that AP-1 is required for retrograde endosome to TGN transport of the receptor...
  12. pmc Targeted disruption of the M(r) 46,000 mannose 6-phosphate receptor gene in mice results in misrouting of lysosomal proteins
    A Koster
    Universitat Gottingen, Germany
    EMBO J 12:5219-23. 1993
    ..The phenotype of MPR46 -/- mice was normal, indicating mechanisms that compensate the MPR46 deficiency in vivo...
  13. ncbi request reprint Mouse cathepsin D gene: molecular organization, characterization of the promoter, and chromosomal localization
    M Hetman
    Universitat Gottingen, Germany
    DNA Cell Biol 13:419-27. 1994
    ..In contrast to the 5' region of human cathepsin D, the murine gene contains three CCAAT boxes but lacks any of the four AP2 binding sites found in the human gene...
  14. ncbi request reprint The function of presenilin-1 in amyloid beta-peptide generation and brain development
    P Saftig
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, , Germany
    Eur Arch Psychiatry Clin Neurosci 249:271-9. 1999
    ....
  15. ncbi request reprint Early embryonic death of mice deficient in gamma-adaptin
    D Zizioli
    Zentrum für Biochemie und Molekulare Zellbiologie, Dept Biochemie II, Universitat Gottingen, Gosslerstrasse 12d, 37073 Gottingen, Germany
    J Biol Chem 274:5385-90. 1999
    ..Free beta1- or micro1 chains were not detectable, indicating that they are unstable unless they are part of AP-1 complexes. Heterozygous mice weigh less then their wild-type littermates and show impaired T cell development...
  16. ncbi request reprint Spermatogenesis in mice is not affected by histone H1.1 deficiency
    S Rabini
    Abt Molekularbiologie, Zentrum Biochemie und Molekulare Zellbiologie, Universitat Gottingen, Humboldtallee 23, Gottingen, 37073, Germany
    Exp Cell Res 255:114-24. 2000
    ..1 mRNA and H1.1 protein. No anatomic abnormalities could be detected. In addition, mice lacking the H1.1 gene were fertile and they showed normal spermatogenesis and testicular morphology...
  17. pmc Mice deficient for the lysosomal proteinase cathepsin D exhibit progressive atrophy of the intestinal mucosa and profound destruction of lymphoid cells
    P Saftig
    Abt Biochemie II, Universitat Gottingen, Germany
    EMBO J 14:3599-608. 1995
    ....
  18. ncbi request reprint Calvarial osteoclasts express a higher level of tartrate-resistant acid phosphatase than long bone osteoclasts and activation does not depend on cathepsin K or L activity
    S Perez-Amodio
    Experimental Periodontology, Academic Center for Dentistry Amsterdam, Universiteit van Amsterdam and Vrije Universiteit, Louwesweg 1, 1066 EA Amsterdam, The Netherlands
    Calcif Tissue Int 79:245-54. 2006
    ....
  19. ncbi request reprint Gene profiling of cathepsin K deficiency in atherogenesis: profibrotic but lipogenic
    S P M Lutgens
    Departments of Pathology, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, P Debyelaan 25, Maastricht, The Netherlands
    J Pathol 210:334-43. 2006
    ..Besides this profibrotic effect, cathepsin K deficiency has a lipogenic effect owing to increased lipid uptake mediated by CD36 and caveolins...
  20. ncbi request reprint Disruption of the cathepsin K gene reduces atherosclerosis progression and induces plaque fibrosis but accelerates macrophage foam cell formation
    E Lutgens
    Department of Pathology, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    Circulation 113:98-107. 2006
    ....
  21. ncbi request reprint Cathepsin K deficiency in pycnodysostosis results in accumulation of non-digested phagocytosed collagen in fibroblasts
    V Everts
    Department of Cell Biology and Histology, Academic Medical Centre, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
    Calcif Tissue Int 73:380-6. 2003
    ..This may explain why cathepsin K-deficient mice lack the dysostotic features that are prominent in patients with pycnodysostosis...
  22. ncbi request reprint The bone lining cell: its role in cleaning Howship's lacunae and initiating bone formation
    V Everts
    Department of Cell Biology and Histology, Academic Medical Centre, University of Amsterdam, The Netherlands
    J Bone Miner Res 17:77-90. 2002
    ....
  23. pmc Presenilin 2 deficiency causes a mild pulmonary phenotype and no changes in amyloid precursor protein processing but enhances the embryonic lethal phenotype of presenilin 1 deficiency
    A Herreman
    Neuronal Cell Biology Laboratory, K U Leuven and Flanders Institute for Biotechnology, 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 96:11872-7. 1999
    ..These results demonstrate in vivo that PS1 and PS2 have partially overlapping functions and that PS1 is essential and PS2 is redundant for normal Notch signaling during mammalian embryological development...
  24. pmc Reduced sperm count and normal fertility in male mice with targeted disruption of the ADP-ribosylation factor-like 4 (Arl4) gene
    A Schurmann
    Institute of Pharmacology, Medical Faculty, Technical University of Aachen, D 52057 Aachen, Germany
    Mol Cell Biol 22:2761-8. 2002
    ..It is suggested that the disruption of Arl4 produces a moderate retardation of germ cell development, possibly at the stage of meiosis...
  25. pmc Phenotype of arylsulfatase A-deficient mice: relationship to human metachromatic leukodystrophy
    B Hess
    Institut für Biochemie II, Georg August Universitat Gottingen, Federal Republic of Germany
    Proc Natl Acad Sci U S A 93:14821-6. 1996
    ..Neurologic examination reveals significant impairment of neuromotor coordination...
  26. ncbi request reprint Involvement of nitric oxide released from microglia-macrophages in pathological changes of cathepsin D-deficient mice
    H Nakanishi
    Laboratory of Oral Aging Science, Division of Oral Biological Sciences, Faculty of Dental Sciences, Kyushu University, Fukuoka 812 8582, Japan
    J Neurosci 21:7526-33. 2001
    ..These results suggest that NO production via iNOS activity in microglia and peripheral macrophages contributes to secondary tissue damages such as neuronal apoptosis and intestinal necrosis, respectively...
  27. pmc Cathepsins B and D are dispensable for major histocompatibility complex class II-mediated antigen presentation
    J Deussing
    Abteilung Innere Medizin I, Albert Ludwigs Universitat Freiburg, Freiburg, 79106 Germany
    Proc Natl Acad Sci U S A 95:4516-21. 1998
    ..Degradation of Ii proceeded normally in Cat B-/- splenocytes, as it did in Cat D-/- cells. We conclude that neither Cat B nor Cat D are essential for MHC class II-mediated antigen presentation...
  28. ncbi request reprint Cathepsin L deficiency as molecular defect of furless: hyperproliferation of keratinocytes and pertubation of hair follicle cycling
    W Roth
    Institut für Molekulare Medizin und Zellforschung, Albert Ludwigs Universitat Freiburg, 79106 Freiburg, Germany
    FASEB J 14:2075-86. 2000
    ..CTSL is the first lysosomal proteinase shown to be essential for epidermal homeostasis and regular hair follicle morphogenesis and cycling...
  29. ncbi request reprint Cathepsin D deficiency induces lysosomal storage with ceroid lipofuscin in mouse CNS neurons
    M Koike
    Department of Cell Biology and Neurosciences, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    J Neurosci 20:6898-906. 2000
    ..These results suggest that the CNS neurons in CD-/- mice show a new form of lysosomal accumulation disease with a phenotype resembling neuronal ceroid lipofuscinosis...
  30. ncbi request reprint Targeted disruption of the lysosomal alpha-mannosidase gene results in mice resembling a mild form of human alpha-mannosidosis
    S Stinchi
    Dipartimento di Biologia Cellulare e Molecolare, Sezione di Biochimica e Biologia Molecolare, Universita degli Studi di Perugia, Via del Giochetto, 06126 Perugia, Italy
    Hum Mol Genet 8:1365-72. 1999
    ..This mouse model will be a valuable tool for studying the pathogenesis of inherited alpha-mannosidosis and may help to evaluate therapeutic approaches for lysosomal storage diseases...
  31. ncbi request reprint Presenilin-1 deficiency leads to loss of Cajal-Retzius neurons and cortical dysplasia similar to human type 2 lissencephaly
    D Hartmann
    Anatomisches Institut der CAU Kiel, Otto Hahn Platz 8, D 24 118, Kiel, Germany
    Curr Biol 9:719-27. 1999
    ..Physiologically, the presenilins are involved in the Notch and Wnt-beta-catenin signaling pathways...
  32. ncbi request reprint Ceramide as an activator lipid of cathepsin D
    M Heinrich
    Institute of Immunology, University of Kiel, Germany
    Adv Exp Med Biol 477:305-15. 2000
    ..Ceramide accumulation in cells derived from A-ceramidase defective Farber patients correlates with enhanced CTSD activity. These findings suggest that A-SMase-derived ceramide targets endolysosomal CTSD...
  33. ncbi request reprint Cathepsin protease activity modulates amyloid load in extracerebral amyloidosis
    C Rocken
    Institute of Pathology, Charite University Hospital, Berlin, Germany
    J Pathol 210:478-87. 2006
    ..CathL was identified as an amyloid-promoting and CathK as an amyloid-retarding cysteine protease. CathB may only modulate the primary structure of the amyloid peptide without affecting amyloid load...