Wolfram Saenger

Summary

Country: Germany

Publications

  1. ncbi The assembly of protein subunits and cofactors in photosystem I
    Wolfram Saenger
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195, Berlin, Germany
    Curr Opin Struct Biol 12:244-54. 2002
  2. ncbi Lipids in photosystem II: interactions with protein and cofactors
    Bernhard Loll
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Biochim Biophys Acta 1767:509-19. 2007
  3. doi Recent progress in the crystallographic studies of photosystem II
    Albert Guskov
    Institute of Chemistry and Biochemistry Crystallography, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Chemphyschem 11:1160-71. 2010
  4. doi Cyanobacterial photosystem II at 2.9-A resolution and the role of quinones, lipids, channels and chloride
    Albert Guskov
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Nat Struct Mol Biol 16:334-42. 2009
  5. doi Citrullination-dependent differential presentation of a self-peptide by HLA-B27 subtypes
    Alessandra Beltrami
    Institut für Immungenetik, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Freie Universitat Berlin, Thielallee 73, 14195 Berlin, Germany
    J Biol Chem 283:27189-99. 2008
  6. pmc Preliminary X-ray diffraction analysis of crystals from the recombinantly expressed human major histocompatibility antigen HLA-B*2704 in complex with a viral peptide and with a self-peptide
    Bernhard Loll
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, 14195 Berlin, Germany
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:939-41. 2005
  7. pmc Crystal structures of N-acetylmannosamine kinase provide insights into enzyme activity and inhibition
    Jacobo Martinez
    From the Institut für Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, 14195 Berlin
    J Biol Chem 287:13656-65. 2012
  8. pmc Crystal structure of monomeric photosystem II from Thermosynechococcus elongatus at 3.6-a resolution
    Matthias Broser
    Institute of Chemistry, Max Volmer Laboratory of Biophysical Chemistry, Technische Universitat Berlin, Berlin, Germany
    J Biol Chem 285:26255-62. 2010
  9. ncbi Crystal structures of HIV-1 Tat-derived nonapeptides Tat-(1-9) and Trp2-Tat-(1-9) bound to the active site of dipeptidyl-peptidase IV (CD26)
    Wilhelm Andreas Weihofen
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    J Biol Chem 280:14911-7. 2005
  10. ncbi Function of two beta-carotenes near the D1 and D2 proteins in photosystem II dimers
    Hiroshi Ishikita
    Institute of Chemistry and Biochemistry Crystallography, Free University of Berlin, Berlin, Germany
    Biochim Biophys Acta 1767:79-87. 2007

Collaborators

Detail Information

Publications79

  1. ncbi The assembly of protein subunits and cofactors in photosystem I
    Wolfram Saenger
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195, Berlin, Germany
    Curr Opin Struct Biol 12:244-54. 2002
    ..Understanding the assembly of the 12 protein subunits and 128 cofactors in photosystem I allows us to study the possible functions of the individual players in this protein-cofactor complex...
  2. ncbi Lipids in photosystem II: interactions with protein and cofactors
    Bernhard Loll
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Biochim Biophys Acta 1767:509-19. 2007
    ..A detailed analysis of lipid-protein and lipid-cofactor interactions allows to derive some general principles of lipid binding pockets in PSII and to suggest possible functional properties of the various identified lipid molecules...
  3. doi Recent progress in the crystallographic studies of photosystem II
    Albert Guskov
    Institute of Chemistry and Biochemistry Crystallography, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Chemphyschem 11:1160-71. 2010
    ..In addition, possible transport channels for water, dioxygen and protons are identified...
  4. doi Cyanobacterial photosystem II at 2.9-A resolution and the role of quinones, lipids, channels and chloride
    Albert Guskov
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Nat Struct Mol Biol 16:334-42. 2009
    ..The chloride position suggests a role in proton-transfer reactions because it is bound through a putative water molecule to the Mn(4)Ca cluster at a distance of 6.5 A and is close to two possible proton channels...
  5. doi Citrullination-dependent differential presentation of a self-peptide by HLA-B27 subtypes
    Alessandra Beltrami
    Institut für Immungenetik, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Freie Universitat Berlin, Thielallee 73, 14195 Berlin, Germany
    J Biol Chem 283:27189-99. 2008
    ....
  6. pmc Preliminary X-ray diffraction analysis of crystals from the recombinantly expressed human major histocompatibility antigen HLA-B*2704 in complex with a viral peptide and with a self-peptide
    Bernhard Loll
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, 14195 Berlin, Germany
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:939-41. 2005
    ..With HLA-B*2705 complexed with pVIPR as a search model, unambiguous molecular-replacement solutions were found for the complexes of HLA-B*2704 with both peptides...
  7. pmc Crystal structures of N-acetylmannosamine kinase provide insights into enzyme activity and inhibition
    Jacobo Martinez
    From the Institut für Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, 14195 Berlin
    J Biol Chem 287:13656-65. 2012
    ..We synthesized a novel inhibitor, 6-O-acetyl-ManNAc, which is more potent than those previously tested. Specific inhibitors of sialic acid biosynthesis may serve to further study biological functions of sialic acid...
  8. pmc Crystal structure of monomeric photosystem II from Thermosynechococcus elongatus at 3.6-a resolution
    Matthias Broser
    Institute of Chemistry, Max Volmer Laboratory of Biophysical Chemistry, Technische Universitat Berlin, Berlin, Germany
    J Biol Chem 285:26255-62. 2010
    ....
  9. ncbi Crystal structures of HIV-1 Tat-derived nonapeptides Tat-(1-9) and Trp2-Tat-(1-9) bound to the active site of dipeptidyl-peptidase IV (CD26)
    Wilhelm Andreas Weihofen
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    J Biol Chem 280:14911-7. 2005
    ..Because the N-terminal sequence of the thromboxane A2 receptor resembles the Trp(2)-Tat-(1-9) peptide, a possible interaction with DPPIV is postulated...
  10. ncbi Function of two beta-carotenes near the D1 and D2 proteins in photosystem II dimers
    Hiroshi Ishikita
    Institute of Chemistry and Biochemistry Crystallography, Free University of Berlin, Berlin, Germany
    Biochim Biophys Acta 1767:79-87. 2007
    ..The calculated Em(CarD1) and Em(ChlZ(D1)) are, respectively, 83 and 126 mV higher than Em(CarD2) and Em(ChlZ(D2)), which could explain why CarD2+ and ChlZ(D2)+ are observed rather than the corresponding CarD1+ and ChlZ(D1)+...
  11. ncbi Redox potentials of chlorophylls in the photosystem II reaction center
    Hiroshi Ishikita
    Institute of Chemistry, Department of Biology, Chemistry, and Pharmacy, Free University of Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Biochemistry 44:4118-24. 2005
    ..The other subunits are responsible for the remaining 80 mV. The high redox potentials of the two accessory Chla Chl(D1/D2) suggests that they also participate in the charge separation process...
  12. ncbi Thermodynamic and structural equivalence of two HLA-B27 subtypes complexed with a self-peptide
    Martin Hülsmeyer
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, 14195 Berlin, Germany
    J Mol Biol 346:1367-79. 2005
    ....
  13. ncbi Structure of the transcription regulator CcpA from Lactococcus lactis
    Bernhard Loll
    Institute for Chemistry and Biochemistry Crystallography, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Acta Crystallogr D Biol Crystallogr 63:431-6. 2007
    ..177 and 0.211, respectively. The presence of a sulfate molecule in the direct vicinity of a putative effector-binding site in the monomer allowed the derivation of a model for the possible binding of small organic effector molecules...
  14. pmc Loss of recognition by cross-reactive T cells and its relation to a C-terminus-induced conformational reorientation of an HLA-B*2705-bound peptide
    Bernhard Loll
    Institut fur Chemie und Biochemie, Abteilung Strukturbiochemie, Freie Universitat Berlin, Takustrasse 6, Berlin 14195, Germany
    Protein Sci 20:278-90. 2011
    ..Together with the Arg-Ser exchange at peptide position 1, there are thus multiple structural reasons that may explain the observed failure of pVIPR-directed, HLA-B*2705-restricted CTL to cross-react with HLA-B*2705:pCAC complexes...
  15. pmc Structural basis for T cell alloreactivity among three HLA-B14 and HLA-B27 antigens
    Pravin Kumar
    Institut für Immungenetik, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Freie Universitat Berlin, Thielallee 73, 14195 Berlin, Germany
    J Biol Chem 284:29784-97. 2009
    ..In contrast, structural alterations between the three complexes with the pLMP2 peptide indicate how heavy chain polymorphisms can influence peptide display and prevent CTL cross-reactivity between HLA-B14 and HLA-B27 antigens...
  16. ncbi Structure of full-length transcription regulator CcpA in the apo form
    Bernhard Loll
    Institute for Chemistry and Biochemistry Crystallography, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Biochim Biophys Acta 1774:732-6. 2007
    ..The structure of the apo CcpA homodimer in the present form is in contrast to other reported structures for CcpA...
  17. doi Crystal structures of human saposins C andD: implications for lipid recognition and membrane interactions
    Maxim Rossmann
    Institut fur Chemie und Biochemie, Kristallographie, Freie Universitat Berlin, Takustr 6, 14195 Berlin, Germany
    Structure 16:809-17. 2008
    ..The crystal structure of SapD suggests that side chains of Lys10 and Arg17 are involved in initial association with the preferred anionic biological membranes by forming salt bridges with sulfate or phosphate lipid headgroups...
  18. pmc Conformational changes within the HLA-A1:MAGE-A1 complex induced by binding of a recombinant antibody fragment with TCR-like specificity
    Pravin Kumar
    Institut für Immungenetik, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Freie Universitat Berlin, Thielallee 73, Berlin 14195, Germany
    Protein Sci 18:37-49. 2009
    ....
  19. doi Crystal structures of two solute receptors for L-cystine and L-cysteine, respectively, of the human pathogen Neisseria gonorrhoeae
    Haydar Bulut
    Institut fuer Chemie und Biochemie Kristallographie, Freie Universitaet Berlin, Takustrasse 6, D 14195 Berlin, Germany
    J Mol Biol 415:560-72. 2012
    ..Moreover, since L-cystine limitation leads to reduction in virulence of N. gonorrhoeae, Ngo0372 might be suited as target for an antimicrobial vaccine...
  20. ncbi Structures of human N-Acetylglucosamine kinase in two complexes with N-Acetylglucosamine and with ADP/glucose: insights into substrate specificity and regulation
    Wilhelm A Weihofen
    Freie Universitat Berlin, Institut fur Chemie und Biochemie Kristallographie, Takustrasse 6, 14195 Berlin Dahlem, Germany
    J Mol Biol 364:388-99. 2006
    ..This suggests that phosphorylation of Tyr205 may modulate GlcNAc kinase activity and/or specificity...
  21. pmc Structural basis of cyanobacterial photosystem II Inhibition by the herbicide terbutryn
    Matthias Broser
    Institut für Chemie Max Volmer Laboratorium für Biophysikalische Chemie, Technische Universitat Berlin, Berlin, Germany
    J Biol Chem 286:15964-72. 2011
    ..Kern, J., Gabdulkhakov, A., Broser, M., Zouni, A., and Saenger, W. (2009) Nat. Struct. Mol. Biol. 16, 334-342). This discovery is discussed in the context of proton transfer to the lumen...
  22. ncbi A major histocompatibility complex-peptide-restricted antibody and t cell receptor molecules recognize their target by distinct binding modes: crystal structure of human leukocyte antigen (HLA)-A1-MAGE-A1 in complex with FAB-HYB3
    Martin Hülsmeyer
    Institut für Chemie Kristallographie, Freie Universitat Berlin, 14195 Berlin, Germany, Cellectis, 93235 Romainville, France
    J Biol Chem 280:2972-80. 2005
    ..The HLA-A1.MAGE-A1.antibody structure is discussed in comparison with those of natural ligands recognizing HLA.peptide complexes...
  23. doi Crystal structures of receptors involved in small molecule transport across membranes
    Haydar Bulut
    Institut fur Chemie und Biochemie, Abteilung Kristallographie, Freie Universitat Berlin, Takustrasse 6, 14195 Berlin, Germany
    Eur J Cell Biol 91:318-25. 2012
    ..The differences in the rather unspecific receptors GacH, MalE and ArtJ are compared with the highly specific receptors AcbH, Ngo0372 and Ngo2014...
  24. ncbi Energetics of a possible proton exit pathway for water oxidation in photosystem II
    Hiroshi Ishikita
    Institute of Chemistry and Biochemistry, Department of Biology, Chemistry, and Pharmacy, Free University of Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Biochemistry 45:2063-71. 2006
    ..Upon removal of the atomic coordinates of PsbO, the pK(a) of these residues are dramatically affected, indicating a significant role of PsbO in tuning the pK(a) of those residues in the proton exit pathway...
  25. pmc How photosynthetic reaction centers control oxidation power in chlorophyll pairs P680, P700, and P870
    Hiroshi Ishikita
    Institute of Chemistry and Biochemistry, Free University Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 103:9855-60. 2006
    ....
  26. ncbi Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem II
    Bernhard Loll
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Nature 438:1040-4. 2005
    ..The structure provides information about the Mn4Ca cluster, where oxidation of water takes place. Our study uncovers near-atomic details necessary to understand the processes that convert light to chemical energy...
  27. ncbi Modeling of variant copies of subunit D1 in the structure of photosystem II from Thermosynechococcus elongatus
    Bernhard Loll
    Institute of Chemistry and Biochemistry Crystallography, Free University Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Biol Chem 389:609-17. 2008
    ..0 A resolution crystal structure of PSII revealed that most of them are in the direct vicinity to redox-active cofactors of the electron transfer chain. Possible structural and mechanistic consequences for electron transfer are discussed...
  28. doi Crystal structures of the solute receptor GacH of Streptomyces glaucescens in complex with acarbose and an acarbose homolog: comparison with the acarbose-loaded maltose-binding protein of Salmonella typhimurium
    Ardeschir Vahedi-Faridi
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Berlin, Germany
    J Mol Biol 397:709-23. 2010
    ....
  29. ncbi Tuning electron transfer by ester-group of chlorophylls in bacterial photosynthetic reaction center
    Hiroshi Ishikita
    Institute of Chemistry, Crystallography, Department of Biology, Chemistry, and Pharmacy, Free University of Berlin, Takustrasse 6, D 14195 Berlin, Germany
    FEBS Lett 579:712-6. 2005
    ..This can be traced back to different orientations of the B(A) ester-group. This tuning ability of chlorophyll redox potentials modulates the electron transfer from SP* to B(A)...
  30. pmc Structures of omega repressors bound to direct and inverted DNA repeats explain modulation of transcription
    Wilhelm Andreas Weihofen
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustr 6, 14195 Berlin, Germany
    Nucleic Acids Res 34:1450-8. 2006
    ..6 A longer in (<---->). These variations grade interactions between adjacent omega2 and explain modulations in cooperative binding affinity of omega2 to operators with different heptad orientations...
  31. pmc Molecular basis for association of PIPKI gamma-p90 with clathrin adaptor AP-2
    Nina Kahlfeldt
    Institute of Chemistry and Biochemistry, Department of Membrane Biochemistry, Freie Universitat Berlin, 14195 Berlin, Germany
    J Biol Chem 285:2734-49. 2010
    ..We postulate a model according to which multiple interactions between PIPKI gamma-p90 and AP-2 lead to spatiotemporally controlled PI(4,5)P(2) synthesis during clathrin-mediated SV endocytosis...
  32. doi The early steps of endocytosis: from cargo selection to membrane deformation
    Yijian Rao
    Institute of Chemistry and Biochemistry, Freie Universitat Berlin, Takustraße 6, 14195 Berlin, Germany
    Eur J Cell Biol 91:226-33. 2012
    ..Here we summarize recent insights gathered from a combination of structural, biochemical, and cell biological studies that have revealed a remarkable complexity of the machinery for endocytic sorting and membrane deformation...
  33. pmc Molecular basis for SH3 domain regulation of F-BAR-mediated membrane deformation
    Yijian Rao
    Institute of Chemistry and Biochemistry, Freie Universitat Berlin, 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 107:8213-8. 2010
    ..Our data thus suggest a structure-based model for SH3-mediated regulation of BAR/F-BAR domain function...
  34. doi Probing the accessibility of the Mn(4)Ca cluster in photosystem II: channels calculation, noble gas derivatization, and cocrystallization with DMSO
    Azat Gabdulkhakov
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Berlin, Germany
    Structure 17:1223-34. 2009
    ..Results from Xe derivatization suggest that the lipid clusters within PSII could serve as a drain for oxygen because of their predominant hydrophobic character and mediate dioxygen release from the lumen...
  35. pmc Purification, crystallization and preliminary X-ray diffraction analysis of the human major histocompatibility antigen HLA-B*2703 complexed with a viral peptide and with a self-peptide
    Bernhard Loll
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, 14195 Berlin, Germany
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:372-4. 2005
    ..Data sets were collected to 1.55 A (B*2703-pVIPR) or 2.0 A (B*2703-pLMP2) resolution using synchrotron radiation. With B*2705-pVIPR as a search model, a clear molecular-replacement solution was found for both B*2703 complexes...
  36. doi HLA class I-associated diseases with a suspected autoimmune etiology: HLA-B27 subtypes as a model system
    Barbara Uchanska-Ziegler
    Institut für Immungenetik, Charité Universitätmedizin Berlin, Campus Benjamin Franklin, Freie Universitat Berlin, Thielallee 73, 14195 Berlin, Germany
    Eur J Cell Biol 91:274-86. 2012
    ....
  37. doi Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition
    Lisa von Kleist
    Institute of Chemistry and Biochemistry and Neurocure Cluster of Excellence, Freie Universitat Berlin, 14195 Berlin, Germany
    Cell 146:471-84. 2011
    ..Pitstops provide new tools to address clathrin function in cell physiology with potential applications as inhibitors of virus and pathogen entry and as modulators of cell signaling...
  38. pmc Structure and function of primase RepB' encoded by broad-host-range plasmid RSF1010 that replicates exclusively in leading-strand mode
    Sebastian Geibel
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 106:7810-5. 2009
    ..Primases that are highly homologous to RepB' are encoded by broad-host-range IncQ and IncQ-like plasmids that share primase initiation sites ssiA and ssiB and high sequence identity with RSF1010...
  39. ncbi Functional role of C(alpha)-H...O hydrogen bonds between transmembrane alpha-helices in photosystem I
    Bernhard Loll
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    J Mol Biol 328:737-47. 2003
    ..The large number of 75 C(alpha)-H...O hydrogen bonds contrasts the 49 conventional hydrogen bonds, indicating that the former and van der Waals contacts determine association and orientation of TMHs in PSI...
  40. ncbi The X-ray crystal structure of human beta-hexosaminidase B provides new insights into Sandhoff disease
    Timm Maier
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, 14195 Berlin, Germany
    J Mol Biol 328:669-81. 2003
    ..The structure reported here is a valid template also for the dimeric structures of beta-hexosaminidase A and S...
  41. ncbi Reinforced HNA backbone hydration in the crystal structure of a decameric HNA/RNA hybrid
    Timm Maier
    Institut für Chemie der Freien Universität Berlin, Takustrasse 6, 14195 Berlin, Germany
    J Am Chem Soc 127:2937-43. 2005
    ..The reinforced backbone hydration is suggested to contribute significantly to the exceptional stability of HNA-containing duplexes and might be one of the causes for the evolutionary preference for ribose-derived nucleic acids...
  42. ncbi Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product
    Maksim Rossocha
    Freie Universitat Berlin, Institut fur Kristallographie, Takustrasse 6, 14195 Berlin, Germany
    Biochemistry 44:5739-48. 2005
    ..The structure analysis of C. perfringens CBAH identifies critical residues in catalysis, substrate recognition, and tetramer formation which may serve in further biochemical characterization of bile acid hydrolases...
  43. ncbi Crystal structure of CD26/dipeptidyl-peptidase IV in complex with adenosine deaminase reveals a highly amphiphilic interface
    Wilhelm A Weihofen
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    J Biol Chem 279:43330-5. 2004
    ..In the crystal structure of porcine DPPIV, the observed tetramer formation was suggested to mediate epithelial and lymphocyte cell-cell adhesion. ADA binding to DPPIV could regulate this adhesion, as it would abolish tetramerization...
  44. ncbi Crystal structure of Homo sapiens protein hp14.5
    Babu A Manjasetty
    Protein Structure Factory, c o BESSY GmbH, Berlin, Germany
    Proteins 54:797-800. 2004
  45. doi Crystal structure of the antitoxin-toxin protein complex RelB-RelE from Methanococcus jannaschii
    Djordje Francuski
    Kristallographie, Institut fur Chemie und Biochemie, Freie Universitat Berlin, Takustrasse 6, Berlin 14195, Germany
    J Mol Biol 393:898-908. 2009
    ..Comparative studies suggest that Asp43 and His79 are also involved in the activity of the toxin...
  46. ncbi Thermostability and Ca2+ binding properties of wild type and heterologously expressed PsbO protein from cyanobacterial photosystem II
    Bernhard Loll
    Department of Chemistry Crystallography, Free University Berlin, 14195 Berlin, Germany
    Biochemistry 44:4691-8. 2005
    ..Our results are discussed in the light of the recent 3D-structural analysis of the oxygen-evolving PSII and structural/thermodynamic differences between the two homologous proteins from thermophilic cyanobacteria and plants...
  47. ncbi Open and closed conformation of the E. coli purine nucleoside phosphorylase active center and implications for the catalytic mechanism
    Gertraud Koellner
    Freie Universitat Berlin, Institut für Chemie Kristallographie, Takustrasse 6, D 14195 Berlin, Germany
    J Mol Biol 315:351-71. 2002
    ..The observation of two kinds of binding sites is fully in line with solution experiments which independently observe strong and weak binding sites for phosphate as well as for the nucleoside inhibitor...
  48. ncbi Cationic state of accessory chlorophyll and electron transfer through pheophytin to plastoquinone in photosystem II
    Hiroshi Ishikita
    Institut fur Chemie und Biochemie, Freie Universitat Berlin, Germany
    Angew Chem Int Ed Engl 45:1964-5. 2006
  49. pmc Dual, HLA-B27 subtype-dependent conformation of a self-peptide
    Martin Hülsmeyer
    Institut fur Kristallographie, Freie Universitat Berlin, 14195 Berlin, Germany
    J Exp Med 199:271-81. 2004
    ....
  50. ncbi Structure of DNA helicase RepA in complex with sulfate at 1.95 A resolution implicates structural changes to an "open" form
    Hai Xu
    Institut fur Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Acta Crystallogr D Biol Crystallogr 59:815-22. 2003
    ....
  51. doi E. coli-based cell-free expression, purification and characterization of the membrane-bound ligand-binding CHASE-TM domain of the cytokinin receptor CRE1/AHK4 of Arabidopsis thaliana
    Klaas Wulfetange
    Institute of Biology Applied Genetics, Dahlem Centre of Plant Sciences DCPS, Freie Universitat Berlin, Albrecht Thaer Weg 6, D 14195, Berlin, Germany
    Mol Biotechnol 47:211-9. 2011
    ..This opens the possibility of further biochemical and pharmacological analysis, as well as structural studies on this type of receptor protein...
  52. pmc Theory of optical spectra of photosystem II reaction centers: location of the triplet state and the identity of the primary electron donor
    Grzegorz Raszewski
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Berlin, Germany
    Biophys J 88:986-98. 2005
    ..The calculations are used to interpret transient optical data. Evidence is provided for the accessory chlorophyll of the D1-branch as being the primary electron donor and the location of the triplet state at low temperatures...
  53. pmc Driving forces of protein association: the dimer-octamer equilibrium in arylsulfatase A
    Peter Vagedes
    Institut fur Chemie, Fachbereich Biologie, Pharmazie, Chemie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Biophys J 83:3066-78. 2002
    ..Application of the proton linkage model to calculate the association energy from the simulated data yielded results identical to the one obtained from the corresponding direct method...
  54. pmc Crystallization and preliminary characterization of three different crystal forms of human saposin C heterologously expressed in Pichia pastoris
    Robert Schultz-Heienbrok
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Germany
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:117-20. 2006
    ..Crystal structures of human saposin C therefore might be the key to mapping out the conformational trajectory of saposin-like proteins...
  55. ncbi Inclusion complexes of V-amylose with undecanoic acid and dodecanol at atomic resolution: X-ray structures with cycloamylose containing 26 D-glucoses (cyclohexaicosaose) as host
    Olaf Nimz
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Carbohydr Res 339:1427-37. 2004
    ..Complexes of polymeric V-amylose with fatty acids and alcohols studied by X-ray fiber diffraction could not provide the here described high resolution...
  56. doi Structural investigation of PsbO from plant and cyanobacterial photosystem II
    Daria Slowik
    Max Volmer Laboratory of Biophysical Chemistry, Technische Universitat Berlin, Strasse des 17, Juni 135, 10623 Berlin, Germany
    J Mol Biol 407:125-37. 2011
    ..The reconstituted PSII was shown to have similar oxygen evolution rates as obtained with wild-type spinach PsbO...
  57. ncbi Modification of biologically active peptides: production of a novel lipohexapeptide after engineering of Bacillus subtilis surfactin synthetase
    Hanka Symmank
    Institut fur Kristallographie, Takustrasse 6, Freie Universitat Berlin, D 14195 Berlin, Germany
    Protein Eng 15:913-21. 2002
    ..The expected lipohexapeptide was produced in vivo and first evidence of a reduced toxicity against erythrocytes and an enhanced lysis of Bacillus licheniformis cells was shown...
  58. pmc Crystal structure of the plasmid maintenance system epsilon/zeta: functional mechanism of toxin zeta and inactivation by epsilon 2 zeta 2 complex formation
    Anton Meinhart
    Institut fur Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 100:1661-6. 2003
    ..To our knowledge, this is the first prokaryotic postsegregational killing system that has been entirely structurally characterized...
  59. ncbi Conformational dimorphism of self-peptides and molecular mimicry in a disease-associated HLA-B27 subtype
    Christine Rückert
    Institut für Immungenetik, Charite Universitatsmedizin Berlin, Campus Virchow Klinikum, Humboldt Universitat zu Berlin, Spandauer Damm 130, 14050 Berlin, Germany
    J Biol Chem 281:2306-16. 2006
    ..These results lend support to the concept that conformational dimorphisms of MHC class I-bound peptides might be connected with the occurrence of self-reactive CTL...
  60. ncbi Crystal structures and mutational analysis of the arginine-, lysine-, histidine-binding protein ArtJ from Geobacillus stearothermophilus. Implications for interactions of ArtJ with its cognate ATP-binding cassette transporter, Art(MP)2
    Ardeschir Vahedi-Faridi
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitaet Berlin, Takustr 6, 14195 Berlin, Germany
    J Mol Biol 375:448-59. 2008
    ..The results are discussed in comparison with known structures of homologous proteins from mesophilic bacteria...
  61. pmc Recognition of DNA by omega protein from the broad-host range Streptococcus pyogenes plasmid pSM19035: analysis of binding to operator DNA with one to four heptad repeats
    Ana B de la Hoz
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, 28049 Madrid, Spain
    Nucleic Acids Res 32:3136-47. 2004
    ....
  62. pmc Functional implications on the mechanism of the function of photosystem II including water oxidation based on the structure of photosystem II
    Petra Fromme
    Max Volmer Laboratorium für Biophysikalische Chemie und Biochemie, Technische Universitat Berlin, Strasse den 17 Juni 135, 10623 Berlin, Germany
    Philos Trans R Soc Lond B Biol Sci 357:1337-44; discussion 1344-5, 1367. 2002
    ..5 A resolution shows that photosystem II lacks the central domain of the photosystem I antenna, which is discussed in respect of the repair cycle of photosystem II due to photoinhibition...
  63. ncbi HLA-B27 subtypes differentially associated with disease exhibit subtle structural alterations
    Martin Hülsmeyer
    Institut für Immungenetik, Universitatsklinikum Charite, Humboldt Universitat zu Berlin, Spandauer Damm 130, 14050 Berlin, Germany
    J Biol Chem 277:47844-53. 2002
    ..These changes induce subtle peptide conformational alterations that may be responsible for the immunobiological differences between these HLA-B27 subtypes...
  64. ncbi The enzyme-binding region of human GM2-activator protein
    Michaela Wendeler
    Kekulé Institut für Organische Chemie und Biochemie der Universität Bonn, Germany
    FEBS J 273:982-91. 2006
    ....
  65. ncbi Comparison of two different lysozyme types under native and crystallization conditions using two-dimensional NMR and dynamic light scattering
    Jaroslaw Poznanski
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Biophys Chem 104:605-16. 2003
    ..According to the published structure of tetragonal lysozyme crystal the largest deviations were found for the residues involved in the intermolecular interactions in crystal structure...
  66. pmc Hexameric RSF1010 helicase RepA: the structural and functional importance of single amino acid residues
    Gunter Ziegelin
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, Dahlem, D 14195 Berlin, Germany
    Nucleic Acids Res 31:5917-29. 2003
    ..Residues K42, E76, D77, D139 and H178, proposed to play key roles in catalyzing the hydrolysis of NTPs, are essential for RepA activity. Residue H178 of motif H3 couples nucleotide consumption to DNA strand separation...
  67. ncbi Thermodynamic and structural analysis of peptide- and allele-dependent properties of two HLA-B27 subtypes exhibiting differential disease association
    Roman C Hillig
    Institut für Immungenetik, Charite, Humboldt Universitat zu Berlin, Spandauer Damm 130, 14050 Berlin, Germany
    J Biol Chem 279:652-63. 2004
    ..The data show that differences in thermodynamic properties between peptide-complexed HLA-B27 subtypes are correlated with a variety of structural properties...
  68. pmc Streptococcus pyogenes pSM19035 requires dynamic assembly of ATP-bound ParA and ParB on parS DNA during plasmid segregation
    Florencia Pratto
    Department of Microbial Biotechnology, National Centre of Biotechnology, CSIC, 28049 Madrid, Spain
    Nucleic Acids Res 36:3676-89. 2008
    ....
  69. ncbi The antenna system of photosystem II from Thermosynechococcus elongatus at 3.2 A resolution
    Bernhard Loll
    Institute of Chemistry Crystallography, Free University Berlin, Takustr 6, 14195 Berlin, Germany
    Photosynth Res 86:175-84. 2005
    ..The chlorophyll a in the lumenal layer are less well conserved between Photosystems I and II and even between CP43 and CP47 with 4 chlorophyll a in the former and 7 in the latter...
  70. ncbi Size determination of cyanobacterial and higher plant photosystem II by gel permeation chromatography, light scattering, and ultracentrifugation
    Athina Zouni
    Max Volmer Laboratorium, Technische Universitat Berlin, Strasse des 17 Juni 135, D 10623 Berlin, Germany
    Biochemistry 44:4572-81. 2005
    ..et al. (2001) Nature 409, 739-743]. Using synchrotron radiation the crystals diffract to a resolution of 3.8 A, which has been improved recently to 3.2 A [Biesiadka, J., et al. (2004) Phys. Chem. Chem. Phys. 6, 4733-4736]...
  71. ncbi RNase T1 variant RV cleaves single-stranded RNA after purines due to specific recognition by the Asn46 side chain amide
    Rico Czaja
    Department of Chemistry, Division of Biochemistry and Molecular Biology, Hamburg University, Martin Luther King Platz 6, 20146 Hamburg, Germany
    Biochemistry 43:2854-62. 2004
    ..In addition to the kinetic studies, these data indicate the participation of Asn46 in the specific recognition of the base and therefore a specific binding of adenosine...
  72. ncbi Where water is oxidized to dioxygen: structure of the photosynthetic Mn4Ca cluster
    Junko Yano
    Melvin Calvin Laboratory, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Science 314:821-5. 2006
    ..The structure of the cluster from the present study is unlike either the 3.0 or 3.5 angstrom-resolution x-ray structures or other previously proposed models...
  73. ncbi Cyanobacterial photosystem II at 3.2 A resolution - the plastoquinone binding pockets
    Jan Kern
    Max Volmer Laboratories for Biophysical Chemistry, Technical University Berlin, Strasse des 17 Juni 135, 10623 Berlin, Germany
    Photosynth Res 84:153-9. 2005
    ..Both binding pockets and problems related to the QB site are discussed here and compared to the situation in the purple bacterial reaction centre...
  74. pmc Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit
    Josef T Kittler
    Department of Physiology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Proc Natl Acad Sci U S A 105:3616-21. 2008
    ..These data define a mechanism for tyrosine kinase regulation of GABA(A)R surface levels and synaptic inhibition...
  75. ncbi In vitro and in vivo stability of the epsilon2zeta2 protein complex of the broad host-range Streptococcus pyogenes pSM19035 addiction system
    Ana G Camacho
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, C S I C, E 28049 Madrid, Spain
    Biol Chem 383:1701-13. 2002
    ..When transcription-translation of a plasmid containing the epsilon and zeta genes was inhibited, cell death was observed after a short lag phase that correlates with the disappearance of the epsilon protein from the background...
  76. pmc X-ray diffraction analysis of crystals from the human major histocompatibility antigen HLA-B*2706 in complex with a viral peptide and with a self-peptide
    Anna Zawacka
    Institut für Immungenetik, Charite Universitatsmedizin Berlin, Campus Virchow Klinikum, Humboldt Universitat zu Berlin, Spandauer Damm 130, 14050 Berlin, Germany
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:1097-9. 2005
    ..83 A (self-peptide pVIPR, RRKWRRWHL; space group P2(1)). Using HLA-B*2705 complexed with the pGR peptide (RRRWHRWRL) as a search model, unambiguous molecular-replacement solutions were found for both HLA-B*2706 complexes...
  77. ncbi Allele-dependent similarity between viral and self-peptide presentation by HLA-B27 subtypes
    Maria Teresa Fiorillo
    Dipartimento di Biologia Cellulare e dello Sviluppo, Universita di Roma La Sapienza, Via dei Sardi 70, 00185 Roma, Italy
    J Biol Chem 280:2962-71. 2005
    ..Furthermore, the results demonstrate that molecular mimicry between pLMP2 and pVIPR in the HLA-B27 context is an allele-dependent property...
  78. ncbi Structure of the Mn4-Ca cluster as derived from X-ray diffraction
    Jan Kern
    Institut fur Chemie, Max Volmer Laboratorium für Biophysikalische Chemie, Sekr PC 14, Technische Universitat Berlin, Strasse des 17 Juni 135, 10623 Berlin, Germany
    Photosynth Res 92:389-405. 2007
    ..Limitations of the presently available structural data are pointed out and possible perspectives for the future are outlined, including the combination of X-ray diffraction and X-ray spectroscopy on single crystals...
  79. pmc Expression, purification and preliminary X-ray crystallographic analysis of the human major histocompatibility antigen HLA-B*1402 in complex with a viral peptide and with a self-peptide
    Pravin Kumar
    Institut für Immungenetik, Charite Universitatsmedizin Berlin, Humboldt Universitat zu Berlin, Thielallee 73, 14195 Berlin, Germany
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:631-4. 2007
    ..55 and 1.86 A resolution, respectively. Unambiguous solutions for both data sets were obtained by molecular replacement using a peptide-complexed HLA-B*2705 molecule (PDB code 1jge) as a search model...