C Wittmann

Summary

Affiliation: Saarland University
Country: Germany

Publications

  1. pmc Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    Appl Environ Microbiol 70:7277-87. 2004
  2. ncbi request reprint Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria
    Christoph Wittmann
    Biochemical Engineering, Saarland University, 66123 Saarbrucken, Germany
    Anal Biochem 327:135-9. 2004
  3. pmc Genealogy profiling through strain improvement by using metabolic network analysis: metabolic flux genealogy of several generations of lysine-producing corynebacteria
    Christoph Wittmann
    Biochemical Engineering Institute, Saarland University, Saarbruecken, Germany
    Appl Environ Microbiol 68:5843-59. 2002
  4. ncbi request reprint Metabolic physiology of aroma-producing Kluyveromyces marxianus
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Saarbruecken, Germany
    Yeast 19:1351-63. 2002
  5. ncbi request reprint Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Im Stadtwald, 66041 Saarbrucken, Germany
    Biotechnol Bioeng 87:1-6. 2004
  6. ncbi request reprint In vivo analysis of intracellular amino acid labelings by GC/MS
    Christoph Wittmann
    Biochemical Engineering Institute, Saarland University, P O Box 151150, 66041 Saarbruecken, Germany
    Anal Biochem 307:379-82. 2002
  7. ncbi request reprint Metabolic flux analysis using mass spectrometry
    C Wittmann
    Biochemical Engineering Institute, Saarland University, Saarbruecken, Germany
    Adv Biochem Eng Biotechnol 74:39-64. 2002
  8. ncbi request reprint Dynamics of intracellular metabolites of glycolysis and TCA cycle during cell-cycle-related oscillation in Saccharomyces cerevisiae
    Christoph Wittmann
    Biochemical Engineering, Saarland University, POB 151150, 66123 Saarbrucken, Germany
    Biotechnol Bioeng 89:839-47. 2005
  9. ncbi request reprint Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    J Biotechnol 132:375-84. 2007
  10. ncbi request reprint Application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum: a novel approach for metabolic flux analysis
    C Wittmann
    Biochemical Engineering Institute, Saarland University, Germany
    Eur J Biochem 268:2441-55. 2001

Collaborators

  • Elmar Heinzle
  • Jean Charles Portais
  • Ursula Rinas
  • Wouter A van Winden
  • P Kiefer
  • Klaus Hollemeyer
  • Priti Talwar
  • J J Heijnen
  • Tobias Fürch
  • Kira J Weissman
  • Ezequiel Franco-Lara
  • Wei Wang
  • Christian Ruckert
  • Alexander Goesmann
  • Jens Olaf Krömer
  • Christoph J Bolten
  • Tae Hoon Yang
  • Hartwig Schröder
  • Gustavo Graciano Fonseca
  • Judith Becker
  • Konstantin Schneider
  • Oskar Zelder
  • Stefanie Kind
  • Rolf Muller
  • Jens O Krömer
  • Cécile Nicolas
  • Susanne Schneiker
  • Fabien Letisse
  • Vidya R Velagapudi
  • Oliver Schilling
  • Corinna Klopprogge
  • Sathish Kumar
  • Gernot T John
  • Michael A Hans
  • M A Hans
  • Weol Kyu Jeong
  • Jeroen Dickschat
  • Andreas Meyerhans
  • Juana Diez
  • Isabel Alves-Rodrigues
  • Andreas K Gombert
  • Silke C Wenzel
  • Yasser A Elnakady
  • Lars Gaigalat
  • Stephane Massou
  • Susanne Wilhelm
  • Sabrina Doss
  • Alfred Pühler
  • Stephan C Schuster
  • Lotte Jelsbak
  • Rosa Martinez-Arias
  • Jose Munoz-Dorado
  • Michelle Merai
  • Armin Ehrenreich
  • Roy D Welch
  • Jorn Kalinowski
  • Edna Bode
  • Maren Kopp
  • Carsten Kegler
  • Andrea Herold
  • Shwan Rachid
  • Maren Scharfe
  • Garret Suen
  • Andreas Karoly Gombert
  • Jomuna V Choudhuri
  • Gregory J Velicer
  • Carolin Groeger
  • Juana Perez
  • Tina Knauber
  • Florenz Sasse
  • Frank Gross
  • Frank Rosenau
  • Christina Herzberg
  • Folker Meyer
  • Olena Perlova
  • Matthias O Altmeyer
  • Olaf Kaiser
  • Sebastian Konietzny
  • Bettina Frank
  • Lars Jelsbak
  • Bukhard Linke
  • Thomas Bekel
  • Jens Krömer
  • Günter Raddatz
  • Daniel Krug
  • Sascha Mormann
  • Philippe Soucaille
  • Helmut Blöcker
  • Silke Pradella
  • Taifo Mahmud

Detail Information

Publications49

  1. pmc Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    Appl Environ Microbiol 70:7277-87. 2004
    ..The high TCA cycle flux and the significant secretion of dihydroxyacetone and glycerol display interesting targets to be approached by genetic engineers for optimization of the strain investigated...
  2. ncbi request reprint Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria
    Christoph Wittmann
    Biochemical Engineering, Saarland University, 66123 Saarbrucken, Germany
    Anal Biochem 327:135-9. 2004
    ..glutamicum. Sampling by quick filtration without quenching allows complete cell separation and authentic quantification of intracellular metabolite pools exhibiting time constants significantly larger than sampling time...
  3. pmc Genealogy profiling through strain improvement by using metabolic network analysis: metabolic flux genealogy of several generations of lysine-producing corynebacteria
    Christoph Wittmann
    Biochemical Engineering Institute, Saarland University, Saarbruecken, Germany
    Appl Environ Microbiol 68:5843-59. 2002
    ..Statistical analysis by a Monte Carlo approach revealed high precision for the estimated fluxes, underlining the fact that the observed differences were clearly strain specific...
  4. ncbi request reprint Metabolic physiology of aroma-producing Kluyveromyces marxianus
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Saarbruecken, Germany
    Yeast 19:1351-63. 2002
    ..marxianus, the approach presented here provides important information on potential targets for genetic optimization of 2-phenylethanol-producing yeasts...
  5. ncbi request reprint Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Im Stadtwald, 66041 Saarbrucken, Germany
    Biotechnol Bioeng 87:1-6. 2004
    ..glutamicum ATCC 21543 compared with ATCC 13287. The present approach has strong potential for broad quantitative screening of metabolic network activities, especially those involving high-cost tracer substrates...
  6. ncbi request reprint In vivo analysis of intracellular amino acid labelings by GC/MS
    Christoph Wittmann
    Biochemical Engineering Institute, Saarland University, P O Box 151150, 66041 Saarbruecken, Germany
    Anal Biochem 307:379-82. 2002
  7. ncbi request reprint Metabolic flux analysis using mass spectrometry
    C Wittmann
    Biochemical Engineering Institute, Saarland University, Saarbruecken, Germany
    Adv Biochem Eng Biotechnol 74:39-64. 2002
    ..The application of MS for flux analysis is illustrated by examples from the literature for various biological systems, including bacteria, fungi, tissue cultures and in vivo studies in humans...
  8. ncbi request reprint Dynamics of intracellular metabolites of glycolysis and TCA cycle during cell-cycle-related oscillation in Saccharomyces cerevisiae
    Christoph Wittmann
    Biochemical Engineering, Saarland University, POB 151150, 66123 Saarbrucken, Germany
    Biotechnol Bioeng 89:839-47. 2005
    ..Oscillations in intracellular amino acids might therefore be, at least partly, following oscillations of their anabolic precursors...
  9. ncbi request reprint Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    J Biotechnol 132:375-84. 2007
    ....
  10. ncbi request reprint Application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum: a novel approach for metabolic flux analysis
    C Wittmann
    Biochemical Engineering Institute, Saarland University, Germany
    Eur J Biochem 268:2441-55. 2001
    ....
  11. ncbi request reprint Characterization and application of an optical sensor for quantification of dissolved O2 in shake-flasks
    Christoph Wittmann
    Biochemical Engineering, Saarland University Am Stadtwald, 66123 Saarbrucken, Germany
    Biotechnol Lett 25:377-80. 2003
    ..Based on the results of this work a simple and straightforward tool is now available for accurate O2 sensing in shake-flasks, which are widely used in microbial cultivations...
  12. ncbi request reprint Modeling and experimental design for metabolic flux analysis of lysine-producing Corynebacteria by mass spectrometry
    C Wittmann
    Biochemical Engineering Institute, Saarland University, 66123 Saarbruecken, Germany
    Metab Eng 3:173-91. 2001
    ..Intensity ratios of other products such as glutamate, valine, phenylalanine, and riboflavin also sensitively reflect flux parameters, which underlines the great potential of mass spectrometry for flux analysis...
  13. ncbi request reprint Investigation of the central carbon metabolism of Sorangium cellulosum: metabolic network reconstruction and quantification of pathway fluxes
    Christoph J Bolten
    Biochemical Engineering, Saarland University, D 66041 Saarbrucken, Germany
    J Microbiol Biotechnol 19:23-36. 2009
    ....
  14. ncbi request reprint Quantification of intracellular amino acids in batch cultures of Saccharomyces cerevisiae
    M A Hans
    Biochemical Engineering Institute, Saarland University, Germany
    Appl Microbiol Biotechnol 56:776-9. 2001
    ..The method presented can be used to investigate the physiology of yeast cultures, including industrially relevant batch and fed-batch processes...
  15. ncbi request reprint Influence of glucose, fructose and sucrose as carbon sources on kinetics and stoichiometry of lysine production by Corynebacterium glutamicum
    P Kiefer
    Biochemical Engineering Institute, Saarland University, Saarbrucken, Germany
    J Ind Microbiol Biotechnol 28:338-43. 2002
    ..On sucrose, specific rates and yields differed significantly from those on fructose and glucose. Transport and metabolism of sucrose, therefore, are not a simple superposition of its building blocks, glucose and fructose...
  16. ncbi request reprint Integrated optical sensing of dissolved oxygen in microtiter plates: a novel tool for microbial cultivation
    Gernot T John
    Biochemical Engineering, Saarland University, P O Box 151150, 66123 Saarbrucken, Germany
    Biotechnol Bioeng 81:829-36. 2003
    ..The resulting maximum oxygen transfer rate was 26 mM h(-1). Simulations showed that the errors caused by the intermittent measurement method were insignificant under the prevailing conditions...
  17. ncbi request reprint Free intracellular amino acid pools during autonomous oscillations in Saccharomyces cerevisiae
    Michael A Hans
    Biochemical Engineering, Saarland University, POB 151150, 66123 Saarbruecken, Germany
    Biotechnol Bioeng 82:143-51. 2003
    ..The fact that there is a close relationship between doubling time and period of oscillation underlines that the described autonomous oscillations are cell-cycle-associated...
  18. pmc In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome
    Jens Olaf Krömer
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    J Bacteriol 186:1769-84. 2004
    ..glutamicum...
  19. pmc Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose
    Patrick Kiefer
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    Appl Environ Microbiol 70:229-39. 2004
    ..Statistical evaluation revealed high precision of the estimates of flux, so the observed differences for metabolic flux are clearly substrate specific...
  20. ncbi request reprint Theoretical aspects of 13C metabolic flux analysis with sole quantification of carbon dioxide labeling
    Tae Hoon Yang
    Biochemical Engineering, Saarland University, Bldg 2, D 66123 Saarbrucken, Germany
    Comput Biol Chem 29:121-33. 2005
    ..The quality of information can be further increased by additional studies with [1,2-13C2] or [1,6-13C2] glucose. Respirometric tracer studies with sole labeling analysis of CO2 are therefore promising for 13C metabolic flux analysis...
  21. ncbi request reprint MALDI-TOF MS for quantification of substrates and products in cultivations of Corynebacterium glutamicum
    C Wittmann
    Biochemical Engineering Institute, Saarland University, POB 151150, 66041 Saarbruecken, Germany
    Biotechnol Bioeng 72:642-7. 2001
    ..glutamicum ATCC 21253, without processing of the sample except a 1:5 dilution. Excellent agreement of the data with conventional techniques like HPLC or enzyme assay was found...
  22. ncbi request reprint Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for metabolic flux analyses using isotope-labeled ethanol
    Klaus Hollemeyer
    Biochemical Engineering Institute, Im Stadtwald, Bldg A 1 5, Saarland University, D 66123 Saarbrucken, Germany
    Rapid Commun Mass Spectrom 21:336-42. 2007
    ..3%...
  23. ncbi request reprint Physiology of the yeast Kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source
    Gustavo Graciano Fonseca
    Universitat des Saarlandes, Biochemical Engineering, Saarbrucken, Germany
    FEMS Yeast Res 7:422-35. 2007
    ..These results provide useful data regarding the future application of K. marxianus in processes aimed at the production of biomass-linked compounds, with high yields and productivities...
  24. doi request reprint Analysis of 13C labeling enrichment in microbial culture applying metabolic tracer experiments using gas chromatography-combustion-isotope ratio mass spectrometry
    Elmar Heinzle
    Biochemical Engineering Institute, Saarland University, 66123 Saarbruecken, Germany
    Anal Biochem 380:202-10. 2008
    ..The current findings indicate a great potential of GC-C-IRMS for labeling quantification in 13C metabolic flux analysis with low labeling degree of tracer substrate directly in larger scale bioreactors...
  25. pmc Metabolite profiling studies in Saccharomyces cerevisiae: an assisting tool to prioritize host targets for antiviral drug screening
    Konstantin Schneider
    Biochemical Engineering Institute, Saarland University, 66123 Saarbrucken, Germany
    Microb Cell Fact 8:12. 2009
    ..abstract:..
  26. ncbi request reprint Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum--over expression and modification of G6P dehydrogenase
    Judith Becker
    Biochemical Engineering, Saarland University, Im Stadtwald, 66123 Saarbrucken, Germany
    J Biotechnol 132:99-109. 2007
    ..Overall a strong apparent NADPH excess resulted. Redox balancing indicated that this excess is completely oxidized by malic enzyme...
  27. pmc Identification and elimination of the competing N-acetyldiaminopentane pathway for improved production of diaminopentane by Corynebacterium glutamicum
    Stefanie Kind
    Biochemical Engineering Institute, Technische Universitat Braunschweig, Gaussstrasse 17, 38106 Braunschweig, Germany
    Appl Environ Microbiol 76:5175-80. 2010
    ..The identification and elimination of the responsible acetyltransferase gene, as presented here, display key contributions of a superior C. glutamicum strain producing diaminopentane as a future building block for bio-based polyamides...
  28. ncbi request reprint Respirometric 13C flux analysis, Part I: design, construction and validation of a novel multiple reactor system using on-line membrane inlet mass spectrometry
    Tae Hoon Yang
    Biochemical Engineering Institute, Saarland University, Bldg A 1 5, Postbox 151150, D 66041 Saarbrucken, Germany
    Metab Eng 8:417-31. 2006
    ..The obtained data were applied for the respirometric (13)C metabolic flux analysis in Part II...
  29. ncbi request reprint Respirometric 13C flux analysis--Part II: in vivo flux estimation of lysine-producing Corynebacterium glutamicum
    Tae Hoon Yang
    Biochemical Engineering Institute, Saarland University, Bldg A 1 5, Postbox 151150, D 66041 Saarbrucken, Germany
    Metab Eng 8:432-46. 2006
    ..Therefore, it is complementary to conventional methods for metabolic flux analysis...
  30. pmc Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources
    Judith Becker
    Biochemical Engineering, Saarland University, Im Stadtwald, 66041 Saarbrucken, Germany
    Appl Environ Microbiol 71:8587-96. 2005
    ..glutamicum. The redirection of flux toward the PPP should also be interesting for the production of other NADPH-demanding compounds as well as for products directly stemming from the PPP...
  31. ncbi request reprint In vivo quantification of intracellular amino acids and intermediates of the methionine pathway in Corynebacterium glutamicum
    Jens Olaf Krömer
    Biochemical Engineering Institute, Saarland University, P O B 151150, 66123 Saarbrucken, Germany
    Anal Biochem 340:171-3. 2005
  32. ncbi request reprint Software tool for automated processing of 13C labeling data from mass spectrometric spectra
    Priti Talwar
    Max Planck Institut für Informatik, Saarbrucken, Germany
    Biotechniques 35:1214-5. 2003
  33. ncbi request reprint Sampling for metabolome analysis of microorganisms
    Christoph J Bolten
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    Anal Chem 79:3843-9. 2007
    ..In such cases a differential approach correcting for medium-contained metabolites is required...
  34. ncbi request reprint Metabolic pathway analysis for rational design of L-methionine production by Escherichia coli and Corynebacterium glutamicum
    Jens Olaf Krömer
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    Metab Eng 8:353-69. 2006
    ....
  35. ncbi request reprint Dynamic calibration and dissolved gas analysis using membrane inlet mass spectrometry for the quantification of cell respiration
    Tae Hoon Yang
    Technische Biochemie, Saarland University, Im Stadtwald, Bldg 2, D 66123 Saarbruecken, Germany
    Rapid Commun Mass Spectrom 17:2721-31. 2003
    ..Hence, dynamic changes in respiration activities could be accurately analyzed using membrane inlet mass spectrometry with the novel calibration method...
  36. ncbi request reprint Quantification of S-adenosyl methionine in microbial cell extracts
    Jens Olaf Krömer
    Biochemical Engineering, Saarland University, Saarbruecken, Germany
    Biotechnol Lett 28:69-71. 2006
    ..Additional quantification of the total homoserine pool, representing both SAM and homoserine, via HPLC allows separate determination of both metabolites...
  37. doi request reprint Physiological response of Corynebacterium glutamicum to oxidative stress induced by deletion of the transcriptional repressor McbR
    Jens O Krömer
    Biochemical Engineering Institute, Saarland University, Saarbrucken, Germany
    Microbiology 154:3917-30. 2008
    ..The storage of these energy-rich polyphosphates is probably the result of a large excess of formation of ATP, as revealed by estimation of the underlying fluxes linked to energy metabolism...
  38. ncbi request reprint Minibioreactors
    Sathish Kumar
    Biochemical Engineering Institute, Saarland University, 66123 Saarbruecken, Germany
    Biotechnol Lett 26:1-10. 2004
    ..g. membrane inlet mass spectrometry and NMR...
  39. pmc Accumulation of homolanthionine and activation of a novel pathway for isoleucine biosynthesis in Corynebacterium glutamicum McbR deletion strains
    Jens Olaf Krömer
    Biochemical Engineering, Saarland University, P O Box 151150, 66123 Saarbrucken, Germany
    J Bacteriol 188:609-18. 2006
    ..Deletion of metB completely prevents homolanthionine accumulation, which is regarded as an important step in the development of C. glutamicum strains for biotechnological methionine production...
  40. ncbi request reprint Towards methionine overproduction in Corynebacterium glutamicum--methanethiol and dimethyldisulfide as reduced sulfur sources
    Christoph J Bolten
    Biochemical Engineering Institute, Technische Universitat Braunschweig, Braunschweig, Germany
    J Microbiol Biotechnol 20:1196-203. 2010
    ..This positive effect was limited by depletion of the metY substrate O-acetylhomoserine, requesting for further metabolic engineering targets towards competitive production strains...
  41. ncbi request reprint Correcting mass isotopomer distributions for naturally occurring isotopes
    Wouter A van Winden
    Department of Bioprocess Technology, Faculty of Applied Sciences, Delft University of Technology, The Netherlands
    Biotechnol Bioeng 80:477-9. 2002
    ..Furthermore, it is shown that the source of information with respect to isotopic compositions of the elements needs to be chosen with care...
  42. ncbi request reprint Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium
    Tobias Fürch
    Technical University Braunschweig HZI Helmholtz Zentrum für Infektionsforschung, Institute of Biochemical Engineering, Inhoffenstrasse 7, D 38124 Braunschweig, Germany
    J Biotechnol 132:385-94. 2007
    ..Thus, pyruvate-dependent chemostat cultivation was identified as a favourable condition for production and secretion of recombinant TFH using B. megaterium as production host...
  43. pmc Transcriptional and metabolic responses of Bacillus subtilis to the availability of organic acids: transcription regulation is important but not sufficient to account for metabolic adaptation
    Oliver Schilling
    Abteilung Allgemeine Mikrobiologie, Institut fur Mikrobiologie und Genetik, Georg August Universitat Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
    Appl Environ Microbiol 73:499-507. 2007
    ..Our results suggest that future work has to concentrate on the posttranslational mechanisms of metabolic regulation...
  44. ncbi request reprint Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates
    Tobias Fürch
    Institute of Biochemical Engineering TU BCE, Technical University Braunschweig HZI Helmholtz Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig, Germany
    Bioprocess Biosyst Eng 30:47-59. 2007
    ....
  45. ncbi request reprint Complete genome sequence of the myxobacterium Sorangium cellulosum
    Susanne Schneiker
    Department of Genetics, Bielefeld University, PO Box 100131, D 33501 Bielefeld, Germany
    Nat Biotechnol 25:1281-9. 2007
    ..Seventeen secondary metabolite loci are encoded in the genome, as well as many enzymes with potential utility in industry...
  46. ncbi request reprint Metabolic flux screening of Saccharomyces cerevisiae single knockout strains on glucose and galactose supports elucidation of gene function
    Vidya R Velagapudi
    VTT Technical Research Centre of Finland, Espoo, Finland
    J Biotechnol 132:395-404. 2007
    ..This allows the conclusion that MAE1 is important for the supply of NADPH during aerobic growth on galactose...
  47. ncbi request reprint Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose-6-phosphate dehydrogenase
    Cécile Nicolas
    UMR5504, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, INRA, INSA, 31400 Toulouse, France
    FEBS Lett 581:3771-6. 2007
    ....
  48. doi request reprint The yeast Kluyveromyces marxianus and its biotechnological potential
    Gustavo Graciano Fonseca
    Department of Chemical Engineering, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil
    Appl Microbiol Biotechnol 79:339-54. 2008
    ....