W Witte

Summary

Affiliation: Robert Koch-Institut
Country: Germany

Publications

  1. doi request reprint Acquired vancomycin resistance in clinically relevant pathogens
    Guido Werner
    FG 13 Nosocomial Infections, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    Future Microbiol 3:547-62. 2008
  2. pmc Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus
    Birgit Strommenger
    Robert Koch Institute, Wernigerode Branch, D 38855 Wernigerode, Germany
    J Clin Microbiol 41:4089-94. 2003
  3. doi request reprint Community-acquired methicillin-resistant Staphylococcus aureus: what do we need to know?
    W Witte
    National Reference Centre for Staphylococci, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    Clin Microbiol Infect 15:17-25. 2009
  4. pmc Nasal colonization of humans with methicillin-resistant Staphylococcus aureus (MRSA) CC398 with and without exposure to pigs
    Christiane Cuny
    Robert Koch Institute, Wernigerode Branc, Wernigerode, Germany
    PLoS ONE 4:e6800. 2009
  5. pmc Rare occurrence of methicillin-resistant Staphylococcus aureus CC130 with a novel mecA homologue in humans in Germany
    Christiane Cuny
    Wernigerode Branch, Robert Koch Institute, Wernigerode, Germany
    PLoS ONE 6:e24360. 2011
  6. pmc Vancomycin-resistant vanB-type Enterococcus faecium isolates expressing varying levels of vancomycin resistance and being highly prevalent among neonatal patients in a single ICU
    Guido Werner
    Unit FG13 Nosocomial Infections, Robert Koch Institute Wernigerode, Wernigerode, Germany
    Antimicrob Resist Infect Control 1:21. 2012
  7. pmc Therapy-refractory Panton Valentine Leukocidin-positive community-acquired methicillin-sensitive Staphylococcus aureus sepsis with progressive metastatic soft tissue infection: a case report
    Joerg C Schefold
    Department of Nephrology and Medical Intensive Care, Charite University Medicine, Campus Virchow Clinic, Berlin, Germany
    J Med Case Reports 1:165. 2007
  8. pmc Antibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria
    Adebayo O Shittu
    Department of Microbiology, Obafemi Awolowo University, Ile Ife, Nigeria
    BMC Microbiol 11:92. 2011
  9. pmc The current MLVA typing scheme for Enterococcus faecium is less discriminatory than MLST and PFGE for epidemic-virulent, hospital-adapted clonal types
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    BMC Microbiol 7:28. 2007
  10. pmc IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    BMC Infect Dis 11:80. 2011

Collaborators

Detail Information

Publications87

  1. doi request reprint Acquired vancomycin resistance in clinically relevant pathogens
    Guido Werner
    FG 13 Nosocomial Infections, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    Future Microbiol 3:547-62. 2008
    ..Nonsusceptibility to glycopeptides also occurs independently from van genes and is a growing therapeutic challenge, especially in MRSA...
  2. pmc Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus
    Birgit Strommenger
    Robert Koch Institute, Wernigerode Branch, D 38855 Wernigerode, Germany
    J Clin Microbiol 41:4089-94. 2003
    ....
  3. doi request reprint Community-acquired methicillin-resistant Staphylococcus aureus: what do we need to know?
    W Witte
    National Reference Centre for Staphylococci, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    Clin Microbiol Infect 15:17-25. 2009
    ..So far, CA-MRSA infections seem to be much less frequent in Europe than in the USA, where patients with particular predispositions and low social status are at especial risk...
  4. pmc Nasal colonization of humans with methicillin-resistant Staphylococcus aureus (MRSA) CC398 with and without exposure to pigs
    Christiane Cuny
    Robert Koch Institute, Wernigerode Branc, Wernigerode, Germany
    PLoS ONE 4:e6800. 2009
    ..The study presented here addresses the question of further transmission to non exposed humans...
  5. pmc Rare occurrence of methicillin-resistant Staphylococcus aureus CC130 with a novel mecA homologue in humans in Germany
    Christiane Cuny
    Wernigerode Branch, Robert Koch Institute, Wernigerode, Germany
    PLoS ONE 6:e24360. 2011
    ..The isolates were negative for enterotoxin genes and tst, as well as for eta, and etb; agr-type was III...
  6. pmc Vancomycin-resistant vanB-type Enterococcus faecium isolates expressing varying levels of vancomycin resistance and being highly prevalent among neonatal patients in a single ICU
    Guido Werner
    Unit FG13 Nosocomial Infections, Robert Koch Institute Wernigerode, Wernigerode, Germany
    Antimicrob Resist Infect Control 1:21. 2012
    ..abstract:..
  7. pmc Therapy-refractory Panton Valentine Leukocidin-positive community-acquired methicillin-sensitive Staphylococcus aureus sepsis with progressive metastatic soft tissue infection: a case report
    Joerg C Schefold
    Department of Nephrology and Medical Intensive Care, Charite University Medicine, Campus Virchow Clinic, Berlin, Germany
    J Med Case Reports 1:165. 2007
    ..Such a condition, which may easily be mistaken for uncomplicated pneumonia, should be treated early, aggressively and over a long period of time in order to avoid relapsing infection...
  8. pmc Antibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria
    Adebayo O Shittu
    Department of Microbiology, Obafemi Awolowo University, Ile Ife, Nigeria
    BMC Microbiol 11:92. 2011
    ..In this study, 68 S. aureus isolates obtained from clinical and non-clinical sources in Nigeria between January and April 2009 were characterized using phenotypic and molecular methods...
  9. pmc The current MLVA typing scheme for Enterococcus faecium is less discriminatory than MLST and PFGE for epidemic-virulent, hospital-adapted clonal types
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    BMC Microbiol 7:28. 2007
    ..All isolates were vancomycin-resistant (vanA type). Typing results for MLVA are compared with results of macrorestriction analysis in PFGE (pulsed-field gel electrophoresis) and MLST (multi-locus sequence typing)...
  10. pmc IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    BMC Infect Dis 11:80. 2011
    ..We describe here performance and predictive value of a single PCR detecting the insertion element, IS16, to identify hospital E. faecium isolates within a collection of 260 strains of hospital, animal and human commensal origins...
  11. ncbi request reprint [Community MRSA]
    W Witte
    Robert Koch Institut, Fachgebiet Nosokomiale Infektionen, Wernigerode
    Zentralbl Chir 132:124-9. 2007
    ..Prevention of further dissemination of caMRSA requires appropriate diagnosis, therapy and sanitation of the carrier state. Hygienic measures have not only to be taken in ambulant treatment but also in households of affected patients...
  12. ncbi request reprint Detection of low-level oxacillin resistance in mecA-positive Staphylococcus aureus
    W Witte
    Robert Koch Institute, Wernigerode, Germany
    Clin Microbiol Infect 13:408-12. 2007
    ..When disk-diffusion was performed on IsoSensitest agar with a zone diameter breakpoint for resistance of <22 mm (as recommended by the Swedish Reference Group for Antibiotics), the sensitivity was 95%...
  13. ncbi request reprint Resistance monitoring of human pathogenic bacteria in Germany, SWOT analysis and examples
    Wolfgang Witte
    National Reference Center for Staphylococci, Robert Koch Institute, Werningerode, Germany
    Int J Med Microbiol 296:63-7. 2006
    ..Trend analysis of the resistance situation in Germany can therefore only be determined through individual studies. These studies are discussed according to a SWOT analysis (SWOT = Strengths, Weaknesses, Opportunities, Threats)...
  14. ncbi request reprint Antibiotic resistance in gram-positive bacteria: epidemiological aspects
    W Witte
    Robert Koch Institute, Wernigerode Branch, Germany
    J Antimicrob Chemother 44:1-9. 1999
    ..pneumoniae, as well as to the spread of resistance genes as exemplified by those causing glycopeptide resistance in enterococci...
  15. ncbi request reprint [Antibiotic-resistant nosocomial pathogens. Part I: diagnostic and typing methods]
    W Witte
    Bereich Wernigerode, Robert Koch Institut, Wernigerode
    Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 47:352-62. 2004
    ..In future the availability of microarray technology will enable routine laboratories to demonstrate particular virulence-associated traits...
  16. ncbi request reprint International dissemination of antibiotic resistant strains of bacterial pathogens
    Wolfgang Witte
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, D 383855 Wernigerode, Germany
    Infect Genet Evol 4:187-91. 2004
    ..Although in vitro transduction has been demonstrated, this gene cluster has only rarely been reported from isolates exhibiting other phage patterns of the same serovar or from other serovars of S. enterica...
  17. ncbi request reprint [Community-based MRSA. A new challenge for dermatology]
    W Witte
    Bereich Wernigerode, Robert Koch Institut
    Hautarzt 56:731-8. 2005
    ..In order to avoid a continuous spread of MRSA, consequent prevention measures both in dermatological practices and for colonized patients in their general environment are essential...
  18. ncbi request reprint Glycopeptide resistant Staphylococcus
    W Witte
    National Reference Center for Staphylococci, Robert Koch Institute, Wernigerode, Germany
    J Vet Med B Infect Dis Vet Public Health 51:370-3. 2004
    ..at the same site of the affected patients could be established. The frequency of VISA (GISA) among MRSA in Central Europe is still low (<0.1%); no VRSA have been detected until now...
  19. pmc Methicillin-resistant Staphylococcus aureus ST398 in humans and animals, Central Europe
    Wolfgang Witte
    Robert Koch Institute, Wernigerode, Germany
    Emerg Infect Dis 13:255-8. 2007
    ..g., dog, pig, horse) in Germany and Austria. Of particular concern is the association of these cases with cases of nosocomial ventilator-associated pneumonia...
  20. ncbi request reprint Methicillin-resistant Staphylococcus aureus containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006
    Wolfgang Witte
    Robert Koch Institute, Wernigerode Branch, 38855 Wernigerode, Germany
    J Antimicrob Chemother 60:1258-63. 2007
    ....
  21. ncbi request reprint Emergence of methicillin-resistant Staphylococcus aureus with Panton-Valentine leukocidin genes in central Europe
    W Witte
    Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, 38855 Wernigerode, Germany
    Eur J Clin Microbiol Infect Dis 24:1-5. 2005
    ..The far-1 gene was shown to be located on the plasmid. One isolate corresponded to community MRSA (cMRSA) of multilocus sequence type 1 from the USA...
  22. doi request reprint Emergence and spread of antibiotic-resistant Gram-positive bacterial pathogens
    Wolfgang Witte
    Robert Koch Institute, Wernigerode Branch, D 38855 Wernigerode, Germany
    Int J Med Microbiol 298:365-77. 2008
    ..Also in Streptococcus pneumoniae, antibiotic resistance is preferentially associated with clonal lineages which have a capacity for spreading. They became, however, more rare after introduction of the 7-valent conjugate vaccine...
  23. ncbi request reprint Ecological impact of antibiotic use in animals on different complex microflora: environment
    W Witte
    Robert Koch Institut, Bereich Wernigerode, Burgstrasse 37, D 38855, Wernigerode, Germany
    Int J Antimicrob Agents 14:321-5. 2000
    ..g. tetR, floR in Salmonella typhimurium DT104). Whether this is due to rare but important transfer events or whether there is a more frequent exchange in aquatic or terrestrial environments needs further elucidation...
  24. ncbi request reprint Selective pressure by antibiotic use in livestock
    W Witte
    Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, D 38855 Wernigerode, Germany
    Int J Antimicrob Agents 16:S19-24. 2000
    ..faecium (GREF) from animals and humans in the community has been recorded. This supports the ban of antibacterial growth promoters that might interfere with human chemotherapy that has been introduced in European Union countries...
  25. ncbi request reprint Characteristics of a new epidemic MRSA in Germany ancestral to United Kingdom EMRSA 15
    W Witte
    Robert Koch Institute, Wernigerode Branch, Germany
    Int J Med Microbiol 290:677-82. 2001
    ..Both share the same Alu I pattern of PCR amplimers of the 3' end region of the coagulase gene. EMRSA 15 and Barnim EMRSA share a common multilocus sequence type indicating a recent, shared evolutionary origin...
  26. ncbi request reprint Subtyping of MRSA isolates belonging to a widely disseminated clonal group by polymorphism of the dru sequences in mec-associated DNA
    W Witte
    Robert Koch Institute, Wernigerode Branch, Germany
    Int J Med Microbiol 291:57-62. 2001
    ..Five different subclones could be discriminated by dru region length polymorphism. Location of deletions and of a few point mutations allow a delineation of these subclones...
  27. doi request reprint Emergence and spread of cfr-mediated multiresistance in staphylococci: an interdisciplinary challenge
    Wolfgang Witte
    Robert Koch Institute, Wernigerode, Germany
    Future Microbiol 6:925-31. 2011
    ..Timely detection and targeted prevention of further dissemination in both human and veterinary medicine is warranted for preserving the activity linezolid as an important antibiotic for treatment of staphylococcal infections...
  28. pmc spa Typing of Staphylococcus aureus as a frontline tool in epidemiological typing
    B Strommenger
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, D 38855 Wernigerode, Germany
    J Clin Microbiol 46:574-81. 2008
    ..They should be selected by dependence on the clonal lineage indicated by spa typing and subsequent BURP analysis as well as on the basis of the particular question to be addressed...
  29. ncbi request reprint Emergence of a new community acquired MRSA strain in Germany
    W Witte
    National Reference Centre for Staphylococci, Robert Koch Institut, Wernigerode, Germany
    Euro Surveill 9:16-8. 2004
    ..The emergence of MRSA with resistance to fusidic acid is a first sign of the emergence of a PVL-positive MRSA clone...
  30. ncbi request reprint Changing pattern of antibiotic resistance in methicillin-resistant Staphylococcus aureus from German hospitals
    W Witte
    Robert Koch Institute, Wernigerode, Germany
    Infect Control Hosp Epidemiol 22:683-6. 2001
    ..The observed changes cannot simply be attributed to overall antibiotic consumption...
  31. ncbi request reprint Obvious lack of association between dynamics of epidemic methicillin-resistant Staphylococcus aureus in central Europe and agr specificity groups
    B Strommenger
    Wernigerode Branch, Robert Koch Institute, Burgstrasse 37, 38855 Wernigerode, Germany
    Eur J Clin Microbiol Infect Dis 23:15-9. 2004
    ..On the basis of the results obtained, it is proposed that the dynamics observed in the population of MRSA in Germany is not due to different agr group specificities in "old" and "new" epidemic clones...
  32. pmc Multiplex PCR for rapid detection of Staphylococcus aureus isolates suspected to represent community-acquired strains
    B Strommenger
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, D 38855 Wernigerode, Germany
    J Clin Microbiol 46:582-7. 2008
    ..The resulting information, in combination with careful interpretation of the epidemiological records, might help to prevent the further spread of those highly virulent caMRSA clones...
  33. doi request reprint [Vancomycin-resistant enterococci (VRE). Recent results and trends in development of antibiotic resistance]
    I Klare
    Nationales Referenzzentrum für Staphylokokken und Enterokokken, Robert Koch Institut, Burgstrasse 37, Wernigerode, Germany
    Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 55:1387-400. 2012
    ..g. reduced EUCAST MIC-breakpoints for glycopeptides; increased use and sensitive performance of chromogenic VRE agars, increased use of molecular diagnostic assays)...
  34. pmc Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing
    B Strommenger
    Wernigerode Branch, Robert Koch Institute, Burgstr 37, D 38855 Wernigerode, and Department of Periodontology, University Hospital Munster, Germany
    J Clin Microbiol 44:2533-40. 2006
    ..aureus epidemiology, especially because of ease of use and the advantages of unambiguous sequence analysis as well as reproducibility and exchange of typing data...
  35. ncbi request reprint Quinupristin/dalfopristin-resistant enterococci of the satA (vatD) and satG (vatE) genotypes from different ecological origins in Germany
    G Werner
    Robert Koch Institute, Wernigerode Branch, Germany
    Microb Drug Resist 6:37-47. 2000
    ..faecium of different ecological origin. A selection of QDRE using the streptogramin virginiamycin S/M as a feed additive and a spread of the resistance via the food chain to humans is probable...
  36. pmc Methicillin-resistant, quinupristin-dalfopristin-resistant Staphylococcus aureus with reduced sensitivity to glycopeptides
    G Werner
    Wernigerode Branch, Robert Koch Institute, D-38855 Wernigerode, Germany
    J Clin Microbiol 39:3586-90. 2001
    ..These groups of isolates were found to be hetero-glycopeptide-intermediate susceptible S. aureus. Information about the failure of glycopeptide chemotherapy has not been available...
  37. ncbi request reprint Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitals
    I Klare
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, 38855, Wernigerode, Germany
    Eur J Clin Microbiol Infect Dis 24:815-25. 2005
    ..In conclusion, it is very important to monitor the occurrence of epidemic-virulent clonal complex-17 strains of E. faecium to prevent their spread in hospitals, especially if they are resistant to glycopeptides and linezolid...
  38. pmc Aminoglycoside-streptothricin resistance gene cluster aadE-sat4-aphA-3 disseminated among multiresistant isolates of Enterococcus faecium
    G Werner
    Wernigerode Branch, Robert Koch Institute, 38855 Wernigerode, Germany
    Antimicrob Agents Chemother 45:3267-9. 2001
    ..Patterns of digested PCR products of aadE-sat4-aphA-3 were identical for all isolates...
  39. doi request reprint A novel IS26 structure surrounds blaCTX-M genes in different plasmids from German clinical Escherichia coli isolates
    A Cullik
    Robert Koch Institute, Burgstrasse 37, 38855 Wernigerode, Germany
    J Med Microbiol 59:580-7. 2010
    ..coli strains. The identical gene arrangement in the bla(CTX-M) neighbourhood in plasmids of different incompatibility groups indicates a main role of IS26 in distribution of mobile resistance elements between different plasmids...
  40. doi request reprint [Current data and trends on methicillin-resistant Staphylococcus aureus (MRSA)]
    F Layer
    Nationales Referenzzentrum für Staphylokokken und Enterokokken, Robert Koch Institut, Burgstrasse 37, Wernigerode, Germany
    Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 55:1377-86. 2012
    ..Identifying common and newly emerging clones is an on-going challenge in the changing epidemiology of MRSA and prevention of further spread needs molecular surveillance...
  41. ncbi request reprint [Methicillin-resistant Staphylococcus aureus--the pathogen]
    W Witte
    Robert Koch-Institut, Bereich Wernigerode, Burgstrasse 37, 38855 Wernigerode
    Kongressbd Dtsch Ges Chir Kongr 118:728-31. 2001
    ..MRSA are not more but also not less virulent than sensitive S. aureus...
  42. ncbi request reprint PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the neighbouring chromosome-borne orfX
    C Cuny
    Robert Koch Institute, Wernigerode, Germany
    Clin Microbiol Infect 11:834-7. 2005
    ..MRSA strains can be identified specifically using one forward primer, with only one or two mismatches, targeting the SCCmec elements of different types, and one reverse primer targeting the orfX region...
  43. ncbi request reprint Linkage of determinants for streptogramin A, macrolide-lincosamide-streptogramin B, and chloramphenicol resistance on a conjugative plasmid in Enterococcus faecium and dissemination of this cluster among streptogramin-resistant enterococci
    G Werner
    Robert Koch Institute, Wernigerode, Germany
    Int J Med Microbiol 290:543-8. 2000
    ....
  44. ncbi request reprint Conjugative transfer of the virulence gene, esp, among isolates of Enterococcus faecium and Enterococcus faecalis
    Claudia Oancea
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, 38855 Wernigerode, Germany
    J Antimicrob Chemother 54:232-5. 2004
    ..This study demonstrates in vitro conjugative transfer of the esp gene among E. faecium and E. faecalis...
  45. pmc Evaluation of a rapid direct assay for identification of bacteria and the mec A and van genes from positive-testing blood cultures
    U Eigner
    Department of Microbiology and Hygeine, Limbach Laboratory, Robert Koch Institute, Im Breitspiel 15, D 69126 Heidelberg, Germany
    J Clin Microbiol 43:5256-62. 2005
    ..The evaluated GenoType blood culture assay showed fast and reliable results in detecting the most important sepsis pathogens and the mec A and van genes directly from positive blood culture bottles...
  46. pmc Multiplexed genotyping of methicillin-resistant Staphylococcus aureus isolates by use of padlock probes and tag microarrays
    Kevin Kurt
    Robert Koch Institut, Burgstr 37, 38855 Wernigerode, Germany
    J Clin Microbiol 47:577-85. 2009
    ....
  47. ncbi request reprint DNA microarray for detection of antibiotic resistance determinants in Bacillus anthracis and closely related Bacillus cereus
    Markus H Antwerpen
    Robert Koch Institut, Burgstrasse 37, 38855 Wernigerode, Germany
    Mol Cell Probes 21:152-60. 2007
    ..The presented assay allows detection of clinically relevant antibiotic resistance determinants within 4h and can be used as a time-saving tool supporting conventional culture-based diagnostics...
  48. doi request reprint [Emergence of clostridium difficile ribotype 027 in Germany: epidemiological and clinical characteristics]
    Andreas Jansen
    Robert Koch Institut, Abteilung für Infektionsepidemiologie, Berlin
    Z Gastroenterol 48:1120-5. 2010
    ..An outbreak investigation was initiated by the local health authorities and the Robert Koch Institute to describe the epidemiology of the possible outbreak and to identify and control the possible sources...
  49. ncbi request reprint Increasing rates of vancomycin resistance among Enterococcus faecium isolated from German hospitals between 2004 and 2006 are due to wide clonal dissemination of vancomycin-resistant enterococci and horizontal spread of vanA clusters
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, D 38855 Wernigerode, Germany
    Int J Med Microbiol 298:515-27. 2008
    ..In conclusion, the increase in the rates of VREF among German hospital patients within the last 2 years might be rather complex and due to different molecular events and scenarios...
  50. ncbi request reprint Molecular analysis of streptogramin resistance in enterococci
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, D 38855 Wernigerode, Germany
    Int J Med Microbiol 292:81-94. 2002
    ..Hybridization and sequencing experiments revealed a linkage of resistance determinants for streptogramins A and B on definite plasmid fragments...
  51. pmc Frequent emergence and limited geographic dispersal of methicillin-resistant Staphylococcus aureus
    Ulrich Nubel
    Robert Koch Institut, 38855 Wernigerode, Germany
    Proc Natl Acad Sci U S A 105:14130-5. 2008
    ....
  52. pmc Influence of transferable genetic determinants on the outcome of typing methods commonly used for Enterococcus faecium
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, 38855 Wernigerode, Germany
    J Clin Microbiol 41:1499-506. 2003
    ..Plasmid fingerprints can be included to subdifferentiate enterococcal isolates possessing identical macrorestriction and PCR typing patterns...
  53. ncbi request reprint Successful termination of a furunculosis outbreak due to lukS-lukF-positive, methicillin-susceptible Staphylococcus aureus in a German village by stringent decolonization, 2002-2005
    Miriam Wiese-Posselt
    Department for Infectious Disease Epidemiology, Robert Koch Institut, Berlin, Germany
    Clin Infect Dis 44:e88-95. 2007
    ..Generally applicable strategies for the control of community outbreaks of furunculosis have not been defined...
  54. ncbi request reprint Intra-hospital dissemination of quinupristin/dalfopristin- and vancomycin-resistant Enterococcus faecium in a paediatric ward of a German hospital
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, 38855 Wernigerode, Germany
    J Antimicrob Chemother 52:113-5. 2003
    ..To demonstrate nosocomial transmission of Enterococcus faecium resistant to quinupristin/dalfopristin and vancomycin/teicoplanin among paediatric patients in a German hospital ward...
  55. pmc Fluoroquinolone resistance and Clostridium difficile, Germany
    Nils Henning Zaiss
    Robert Koch Institute, Burgstrasse 37, 38855 Wernigerode, Germany
    Emerg Infect Dis 16:675-7. 2010
    ..Fluoroquinolone resistance and associated gyrase mutations were frequent in various ribotypes, but no resistance to metronidazole or vancomycin was noted...
  56. pmc Molecular detection of linezolid resistance in Enterococcus faecium and Enterococcus faecalis by use of 5' nuclease real-time PCR compared to a modified classical approach
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, D 38855 Wernigerode, Germany
    J Clin Microbiol 42:5327-31. 2004
    ..Results were confirmed by a classical approach involving LabChip technology assayed with an Agilent Bioanalyzer 2100...
  57. doi request reprint High-level ciprofloxacin resistance among hospital-adapted Enterococcus faecium (CC17)
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    Int J Antimicrob Agents 35:119-25. 2010
    ..Their allocation to specific MLST and pulsed-field gel electrophoresis (PFGE) types revealed differences in the emergence and spread of corresponding mutations and strains...
  58. pmc Evaluation of new broth media for microdilution antibiotic susceptibility testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria
    Ingo Klare
    Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, D 38855 Wernigerode, Germany
    Appl Environ Microbiol 71:8982-6. 2005
    ....
  59. ncbi request reprint Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and their relationship to human isolates
    Birgit Strommenger
    Robert Koch Institute, Wernigerode Branch, D 38855 Wernigerode, Germany
    J Antimicrob Chemother 57:461-5. 2006
    ..Methicillin-resistant Staphylococcus aureus (MRSA) isolates from pet animals were characterized and compared with human isolates from clonal complexes most prevalent in Central Europe...
  60. pmc Population structure of Francisella tularensis
    Ulrich Nubel
    Robert Koch Institute, Burgstr 37, 38855 Wernigerode, Germany
    J Bacteriol 188:5319-24. 2006
    ..novicida and F. tularensis subsp. mediasiatica were indicated to be phylogenetically incoherent taxa. Incongruent gene trees and mosaic structures of housekeeping genes provided evidence for genetic recombination in F. tularensis...
  61. ncbi request reprint DNA microarray for the detection of therapeutically relevant antibiotic resistance determinants in clinical isolates of Staphylococcus aureus
    Birgit Strommenger
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, D 38855 Wernigerode, Germany
    Mol Cell Probes 21:161-70. 2007
    ..It is easily expandable and thus can be adapted to changing clinical and epidemiological requirements in clinical diagnosis as well as in epidemiological studies...
  62. ncbi request reprint Linkage of erm(B) and aadE-sat4-aphA-3 in multiple-resistant Enterococcus faecium isolates of different ecological origins
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, D 38855 Wernigerode, Germany
    Microb Drug Resist 9:S9-16. 2003
    ..A 17-kb composite cluster was identified in a single hospital isolate linking determinants for glycopeptide (vanA), macrolide-lincosamide-streptogramin B [erm(B)], and aminoglycoside-streptothricin (aadE-sat4-aphA-3) resistances...
  63. ncbi request reprint Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use
    Ingo Klare
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    J Antimicrob Chemother 59:900-12. 2007
    ..To propose tentative epidemiological cut-off (ECOFF) values for recognizing intrinsic and acquired antimicrobial resistances in numerically dominant species...
  64. ncbi request reprint Occurrence and spread of antibiotic resistances in Enterococcus faecium
    Ingo Klare
    Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, D 38855 Wernigerode, Germany
    Int J Food Microbiol 88:269-90. 2003
    ..In order to prevent the dissemination of multiple antibiotic-resistant enterococci or their transferable resistance genes, a prudent use of antibiotics is necessary in human and veterinary medicine, and in animal husbandry...
  65. ncbi request reprint Molecular ecological studies on spread of antibiotic resistance genes
    Wolfgang Witte
    Robert Koch Institute, Wernigerode Branch, Germany
    Anim Biotechnol 13:57-70. 2002
  66. ncbi request reprint Occurrence of antibiotic-resistant enterobacteria in agricultural foodstuffs
    Sybille Boehme
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    Mol Nutr Food Res 48:522-31. 2004
    ..In conclusion, a contribution of sprouts contaminated with multiresistant, Gram-negative enterobacteria to a common gene pool among human commensal and pathogenic bacteria cannot be excluded...
  67. ncbi request reprint A case of Clostridium difficile-associated disease due to the highly virulent clone of Clostridium difficile PCR ribotype 027, March 2007 in Germany
    N H Zaiss
    Robert Koch Institute, Wernigerode, Germany
    Euro Surveill 12:E071115.1. 2007
  68. pmc A timescale for evolution, population expansion, and spatial spread of an emerging clone of methicillin-resistant Staphylococcus aureus
    Ulrich Nubel
    Robert Koch Institut, Wernigerode, Germany
    PLoS Pathog 6:e1000855. 2010
    ..In addition, comparative genomics indicated complex bacteriophage dynamics...
  69. doi request reprint Clusters of infections in horses with MRSA ST1, ST254, and ST398 in a veterinary hospital
    Christiane Cuny
    Wernigerode Branch, Robert Koch Institute, Wernigerode, Germany
    Microb Drug Resist 14:307-10. 2008
    ..The infections were caused by different MRSA clones (ST1, ST254, and ST398). In the same time, nasal colonization of veterinarians, veterinary personnel, and students was observed indicating transmission to humans...
  70. doi request reprint Emergence of methicillin-resistant Staphylococcus aureus (MRSA) in different animal species
    Christiane Cuny
    Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, 38855 Wernigerode, Germany
    Int J Med Microbiol 300:109-17. 2010
    ..Mechanisms of host adaptation and their genomic background are poorly understood so far...
  71. doi request reprint Resistance to cephalosporins and carbapenems in Gram-negative bacterial pathogens
    Yvonne Pfeifer
    Robert Koch Institute, Nosocomial Infections, Wernigerode Branch, Burgstr 37, 38855 Wernigerode, Germany
    Int J Med Microbiol 300:371-9. 2010
    ..This mini-review summarizes the historical emergence of beta-lactam resistance and beta-lactamases as major resistance mechanism in enteric bacteria, and also highlights recent developments such as multidrug- and carbapenem resistance...
  72. pmc Molecular epidemiology of hospital-acquired vancomycin-resistant enterococci
    Marianne Abele-Horn
    Institute for Hygiene and Microbiology, University of Wurzburg, Germany
    J Clin Microbiol 44:4009-13. 2006
    ..The combination of MLST and MLVA may be an alternative to PFGE in hospital epidemiology, providing the benefits of high accuracy, reproducibility, and portability...
  73. pmc Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management
    Dag Harmsen
    Institute for Hygiene, University of Munster, Munster, Germany
    J Clin Microbiol 41:5442-8. 2003
    ..spa typing was valuable for tracking of epidemic isolates. The data show that disproval of epidemiologically suggested transmissions of MRSA is one of the main objectives of spa typing in departments with a high incidence of MRSA...
  74. pmc Clonal analysis of Staphylococcus epidermidis isolates carrying or lacking biofilm-mediating genes by multilocus sequence typing
    Svetlana Kozitskaya
    Institut für Molekulare Infektionsbiologie, Wurzburg, Germany
    J Clin Microbiol 43:4751-7. 2005
    ..It is hypothesized that the successful establishment of ST27 within nosocomial environments has been facilitated by the presence of genes encoding biofilm and resistance traits...
  75. ncbi request reprint In vitro activity of daptomycin versus linezolid and vancomycin against gram-positive uropathogens and ampicillin against enterococci, causing complicated urinary tract infections
    Florian M E Wagenlehner
    Department of Urology, St Elisabeth Hospital, Straubing, Germany
    Chemotherapy 51:64-9. 2005
    ..The existing therapeutic options for complicated urinary tract infections (UTI) caused by gram-positive uropathogens are not always optimal. Therefore, newer antimicrobials have to be assessed...
  76. pmc Methicillin-resistant Staphylococcus aureus in Europe, 1999-2002
    Edine W Tiemersma
    National Institute for Public Health and the Environment, Bilthoven, The Netherlands
    Emerg Infect Dis 10:1627-34. 2004
    ..The observed trends should stimulate initiatives to control MRSA at national, regional, and hospital levels. The large differences between hospitals indicate that efforts may be most effective at regional and hospital levels...
  77. pmc Evaluation of molecular typing methods in characterizing a European collection of epidemic methicillin-resistant Staphylococcus aureus strains: the HARMONY collection
    Barry D Cookson
    Laboratory of Hospital Infection, Centre for Infections, Health Protection Agency, London, United Kingdom
    J Clin Microbiol 45:1830-7. 2007
    ....
  78. doi request reprint Staphylococcus aureus in dermatology outpatients with special emphasis on community-associated methicillin-resistant strains
    Uta Jappe
    Department of Dermatology, University of Heidelberg, Heidelberg, Germany
    J Invest Dermatol 128:2655-64. 2008
    ..The significant risk factor for the acquisition of CA-MRSA was visits to foreign countries and/or professional or private contacts with foreigners...
  79. pmc Detection of mutations conferring resistance to linezolid in Enterococcus spp. by fluorescence in situ hybridization
    Guido Werner
    Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert Kochstr 8, D 89081 Ulm, Germany
    J Clin Microbiol 45:3421-3. 2007
    ..The assay was evaluated with 106 Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify a single mutated allele in phenotypically linezolid-susceptible isolates...
  80. ncbi request reprint Impact of antibiotics on conjugational resistance gene transfer in Staphylococcus aureus in sewage
    Knut Ohlsen
    Institute for Molecular Biology of Infectious Diseases, The University of Würzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    Environ Microbiol 5:711-6. 2003
    ..1x10(-5)-5.0x10(-8). However, low-level concentrations of antibiotics measured in sewage are below concentrations that can increase plasmid transfer frequencies of gentamicin resistance plasmids of staphylococci...
  81. ncbi request reprint Intercenter reproducibility of binary typing for Staphylococcus aureus
    Willem B van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 51:19-28. 2002
    ..The current BT protocol thus represents a simple method generating robust, reproducible genotype data for S. aureus strains...
  82. ncbi request reprint Are there regional variations in the diagnosis, surveillance, and control of methicillin-resistant Staphylococcus aureus?
    Hervé M Richet
    Investigation and Prevention Branch, Hospital Infections Program, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Infect Control Hosp Epidemiol 24:334-41. 2003
    ..To assess the way healthcare facilities (HCFs) diagnose, survey, and control methicillin-resistant Staphylococcus aureus (MRSA)...
  83. pmc Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spre
    Stephen Murchan
    Laboratory of Hospital Infection, Central Public Health Laboratory, London NW9 5HT, United Kingdom
    J Clin Microbiol 41:1574-85. 2003
    ..This highlights the need for closer international collaboration to monitor the spread of current epidemic strains as well as the emergence of new ones...
  84. doi request reprint Tigecycline-resistant Enterococcus faecalis strain isolated from a German intensive care unit patient
    Guido Werner
    J Antimicrob Chemother 61:1182-3. 2008
  85. pmc Genotypic diversity, antimicrobial resistance, and virulence factors of human isolates and probiotic cultures constituting two intraspecific groups of Enterococcus faecium isolates
    Vanessa Vankerckhoven
    Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    Appl Environ Microbiol 74:4247-55. 2008
    ..In combination with antimicrobial resistance and virulence testing, this grouping might represent an additional criterion in assessing the safety of new potential probiotic E. faecium isolates...
  86. ncbi request reprint [Importance of the spread of Methicillin-resistant Staphylococcus aureus (MRSA) in fattened pigs for humans?]
    Christiane Cuny
    Robert Koch Institut, Bereich Wernigerode
    MMW Fortschr Med 150:65-7. 2008
  87. ncbi request reprint How many infections are caused by patient-to-patient transmission in intensive care units?
    Hajo Grundmann
    European Antimicrobial Resistance Surveillance System, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
    Crit Care Med 33:946-51. 2005
    ..Such information would be useful for the implementation of appropriate infection control measures...