Peter Lasch

Summary

Affiliation: Robert Koch-Institut
Country: Germany

Publications

  1. ncbi request reprint Molecular changes of preclinical scrapie can be detected by infrared spectroscopy
    Janina Kneipp
    PG3, Robert Koch Institut, D 13353 Berlin, Germany
    J Neurosci 22:2989-97. 2002
  2. ncbi request reprint Imaging of colorectal adenocarcinoma using FT-IR microspectroscopy and cluster analysis
    Peter Lasch
    Department of Chemistry and Biochemistry, City University of New York, Hunter College, 695 Park Avenue, New York, NY 10021, USA
    Biochim Biophys Acta 1688:176-86. 2004
  3. ncbi request reprint Spatial resolution in infrared microspectroscopic imaging of tissues
    Peter Lasch
    P25 Biomedical Spectroscopy, 13353 Berlin, Nordufer 20, Germany
    Biochim Biophys Acta 1758:814-29. 2006
  4. ncbi request reprint Detection of preclinical scrapie from serum by infrared spectroscopy and chemometrics
    Peter Lasch
    P25 Biomedical Spectroscopy, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Anal Bioanal Chem 387:1791-800. 2007
  5. ncbi request reprint Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion research
    Michael Beekes
    P24 Transmissible Spongiform Encephalopathies, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
    Vet Microbiol 123:305-19. 2007
  6. pmc ATR-FTIR spectroscopy reveals genomic loci regulating the tissue response in high fat diet fed BXD recombinant inbred mouse strains
    Ayca Dogan
    Department for Crop and Animal Sciences, Humboldt Universitat zu Berlin, Invalidenstraße 42, 10115, Berlin, Germany
    BMC Genomics 14:386. 2013
  7. doi request reprint MALDI-TOF mass spectrometry compatible inactivation method for highly pathogenic microbial cells and spores
    Peter Lasch
    P25, ZBS2, and ZBS4, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Anal Chem 80:2026-34. 2008
  8. doi request reprint Correction of axial chromatic aberrations in confocal Raman microspectroscopic measurements of a single microbial spore
    Peter Lasch
    P25 Biomedical Spectroscopy, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Analyst 134:1162-70. 2009
  9. pmc Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks
    Peter Lasch
    P25, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Appl Environ Microbiol 75:7229-42. 2009
  10. doi request reprint Characterization of Yersinia using MALDI-TOF mass spectrometry and chemometrics
    Peter Lasch
    Biomedical Spectroscopy P 25, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Anal Chem 82:8464-75. 2010

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Molecular changes of preclinical scrapie can be detected by infrared spectroscopy
    Janina Kneipp
    PG3, Robert Koch Institut, D 13353 Berlin, Germany
    J Neurosci 22:2989-97. 2002
    ....
  2. ncbi request reprint Imaging of colorectal adenocarcinoma using FT-IR microspectroscopy and cluster analysis
    Peter Lasch
    Department of Chemistry and Biochemistry, City University of New York, Hunter College, 695 Park Avenue, New York, NY 10021, USA
    Biochim Biophys Acta 1688:176-86. 2004
    ..Among the cluster imaging methods, AH clustering (Ward's algorithm) proved to be the best method in terms of tissue structure differentiation...
  3. ncbi request reprint Spatial resolution in infrared microspectroscopic imaging of tissues
    Peter Lasch
    P25 Biomedical Spectroscopy, 13353 Berlin, Nordufer 20, Germany
    Biochim Biophys Acta 1758:814-29. 2006
    ..Based on synchrotron IR microspectroscopic data we have furthermore demonstrated that the technique of 3D-FSD can be successfully applied to increase the spatial resolution in a real IR imaging setup...
  4. ncbi request reprint Detection of preclinical scrapie from serum by infrared spectroscopy and chemometrics
    Peter Lasch
    P25 Biomedical Spectroscopy, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Anal Bioanal Chem 387:1791-800. 2007
    ..Possible spectral markers responsible for the discrimination capacity of the two different ANNs are discussed...
  5. ncbi request reprint Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion research
    Michael Beekes
    P24 Transmissible Spongiform Encephalopathies, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
    Vet Microbiol 123:305-19. 2007
    ..This article sets out to give a review of current examples of the analytical potential of FT-IR spectroscopy in microbiology and prion research...
  6. pmc ATR-FTIR spectroscopy reveals genomic loci regulating the tissue response in high fat diet fed BXD recombinant inbred mouse strains
    Ayca Dogan
    Department for Crop and Animal Sciences, Humboldt Universitat zu Berlin, Invalidenstraße 42, 10115, Berlin, Germany
    BMC Genomics 14:386. 2013
    ..In the present study, the analytical method of ATR-FTIR spectroscopy has been combined with a genetic approach to identify genetic differences responsible for phenotypic alterations in adipose, liver and muscle tissues...
  7. doi request reprint MALDI-TOF mass spectrometry compatible inactivation method for highly pathogenic microbial cells and spores
    Peter Lasch
    P25, ZBS2, and ZBS4, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Anal Chem 80:2026-34. 2008
    ..This MALDI-TOF/ICMS compatible sample preparation protocol is simple and rapid (30 min) and assures reliable inactivation of vegetative cells and spores of highly pathogenic (BSL-3) microorganisms...
  8. doi request reprint Correction of axial chromatic aberrations in confocal Raman microspectroscopic measurements of a single microbial spore
    Peter Lasch
    P25 Biomedical Spectroscopy, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Analyst 134:1162-70. 2009
    ..In this paper we will also present cluster mean spectra which will be discussed in light of the presence of proteins and Ca-DPA, a calcium chelate of dipicolinic acid in the spore...
  9. pmc Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks
    Peter Lasch
    P25, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Appl Environ Microbiol 75:7229-42. 2009
    ..Superior classification accuracy was achieved when supervised ANNs were employed. For the identification of B. anthracis, independent validation of optimized ANN models yielded a diagnostic sensitivity of 100% and a specificity of 100%...
  10. doi request reprint Characterization of Yersinia using MALDI-TOF mass spectrometry and chemometrics
    Peter Lasch
    Biomedical Spectroscopy P 25, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Anal Chem 82:8464-75. 2010
    ..In this way, the Y. pestis-specific biomarker at m/z 3065 could be identified as a fragment of the plasmid-encoded plasminogen activator, one of the major virulence factors in plague infections...
  11. ncbi request reprint Spatially resolved IR microspectroscopy of single cells
    Peter Lasch
    Department of Chemistry, Hunter College and Graduate Center of the City University of New York, 695 Park Avenue, New York, New York 10021, USA
    Biopolymers 67:335-8. 2002
    ..A comparison of the spectra of the two cell lines yielded only insignificant spectral differences, indicating that IR spectroscopy monitors the overall cell activity rather than specific signs of cancer...
  12. ncbi request reprint Analysis of biofluids in aqueous environment based on mid-infrared spectroscopy
    Heinz Fabian
    Robert Koch Institute, P13, Nordufer 20, 13353 Berlin, Germany
    J Biomed Opt 10:031103. 2005
    ..Based on these results, the advantages and limitations of the liquid sample technique and the dried film approach for routine analysis of biofluids are discussed...
  13. ncbi request reprint FTIR-microspectroscopy of prion-infected nervous tissue
    Ariane Kretlow
    P25, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Biochim Biophys Acta 1758:948-59. 2006
    ....
  14. ncbi request reprint In situ identification of protein structural changes in prion-infected tissue
    Janina Kneipp
    Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Biochim Biophys Acta 1639:152-8. 2003
    ..No evidence of these structural changes was observed in normal neurons...
  15. ncbi request reprint IR spectra and IR spectral maps of individual normal and cancerous cells
    Max Diem
    Department of Chemistry, City University of New York, Hunter College, 695 Park Avenue, New York, New York 10021, USA
    Biopolymers 67:349-53. 2002
    ..These spectral variations are independent of the state of health of a cell...
  16. pmc Infrared microspectroscopy detects protein misfolding cyclic amplification (PMCA)-induced conformational alterations in hamster scrapie progeny seeds
    Martin L Daus
    From FG 14 AG 5 Unconventional Pathogens and Their Inactivation, Applied Infection Control and Hospital Hygiene, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany and
    J Biol Chem 288:35068-80. 2013
    ..Thus, strain modifications of 263K prions induced by PMCA seem to have been partially reversed when PMCA products were reinoculated into the original host species. ..
  17. ncbi request reprint Diagnosing benign and malignant lesions in breast tissue sections by using IR-microspectroscopy
    Heinz Fabian
    Robert Koch Institute, P25, Nordufer 20, 13353 Berlin, Germany
    Biochim Biophys Acta 1758:874-82. 2006
    ..Based on these results, the potential of the IR-microspectroscopic approach for diagnosing breast tissue lesions is discussed...
  18. ncbi request reprint Antemortem identification of bovine spongiform encephalopathy from serum using infrared spectroscopy
    Peter Lasch
    P13, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Anal Chem 75:6673-8. 2003
    ..The combination of classification algorithms increased the sensitivity and specificity of BSE detection to 96 and 92%, respectively...
  19. ncbi request reprint Infrared spectroscopy of human cells and tissue: detection of disease
    Peter Lasch
    Department of Chemistry and Biochemistry, City University of New York, Hunter College, 695 Park Avenue, New York, NY 10021, USA
    Technol Cancer Res Treat 1:1-7. 2002
    ..The false color maps reveal the same anatomical features of the tissue that can be confirmed using a variety of common histopathological procedures, and may be used to differentiate between normal and diseased areas of the tissue...
  20. doi request reprint Phenotypic heterogeneity within microbial populations at the single-cell level investigated by confocal Raman microspectroscopy
    Antje Hermelink
    P25 Biomedical Spectroscopy, Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
    Analyst 134:1149-53. 2009
    ..By the use of three different test organisms as model systems, we show pronounced cellular heterogeneity even in colonies cultivated under laboratory conditions...
  21. doi request reprint Multiplex detection of microbial and plant toxins by immunoaffinity enrichment and matrix-assisted laser desorption/ionization mass spectrometry
    Skadi Kull
    Biomedical Spectroscopy P25, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Anal Chem 82:2916-24. 2010
    ..Furthermore, the MALDI-based multiplex assay allowed for the discrimination of closely related BoNT sero- and subtypes, including a real case of food-borne botulism in Germany...
  22. ncbi request reprint Identification of scrapie infection from blood serum by Fourier transform infrared spectroscopy
    Jürgen Schmitt
    FB VI, Spectroscopy Group, University of Trier, Universitätsring, Germany
    Anal Chem 74:3865-8. 2002
    ..The predictive value of a positive (negative) test was 100% (98%). The proposed serum test circumvents substantial drawbacks of conventional TSE diagnostics and can be fully automated...
  23. pmc Electric field-induced changes in lipids investigated by modulated excitation FTIR spectroscopy
    Michael Schwarzott
    Institute of Physical Chemistry, University of Vienna, A 1090 Vienna, Austria
    Biophys J 86:285-95. 2004
    ..Young's elasticity modulus of DMPC could be calculated to be E( perpendicular ) = 2.2 x 10(6) Pa +/- 1.8 x 10(6) Pa, which is in good agreement with published data obtained by electric field-dependent capacitance measurements...
  24. pmc Early alterations in myocardia and vessels of the diabetic rat heart: an FTIR microspectroscopic study
    Neslihan Toyran
    Department of Biological Sciences, Middle East Technical University, 06531 Ankara, Turkey
    Biochem J 397:427-36. 2006
    ..The present study for the first time demonstrated the diabetes-induced alterations at molecular level in both ventricle myocardia and the veins in relatively short-term diabetes...