Research Topics
| Silke R KleeSummaryAffiliation: Robert Koch-Institut Country: Germany Publications
| Collaborators
|
Detail Information
Publications
Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetiiSilke R Klee
Robert Koch Institut, Centre for Biological Safety, Nordufer 20, 13353 Berlin, Germany
BMC Microbiol 6:2. 2006..We developed and evaluated TaqMan-based real-time PCR assays that targeted the singular icd (isocitrate dehydrogenase) gene and the transposase of the IS1111a element present in multiple copies in the C. burnetii genome...
Evaluation of different methods to discriminate Bacillus anthracis from other bacteria of the Bacillus cereus groupS R Klee
Robert Koch Institut, Centre for Biological Safety 2, Berlin, Germany
J Appl Microbiol 100:673-81. 2006..To evaluate different methods that are useful for rapid and definitive discrimination of Bacillus anthracis from other bacteria of the Bacillus cereus group in environmental samples like letters claimed to contain anthrax spores...
Characterization of Bacillus anthracis-like bacteria isolated from wild great apes from Cote d'Ivoire and CameroonSilke R Klee
Robert Koch Institut, Centre for Biological Safety 2, Nordufer 20, 13353 Berlin, Germany
J Bacteriol 188:5333-44. 2006..We propose that the newly discovered atypical B. anthracis strains share a common ancestor with classic B. anthracis or that they emerged recently by transfer of the B. anthracis plasmids to a strain of the B. cereus group...
Evaluation of a real-time PCR assay to detect Coxiella burnetiiSilke R Klee
Robert Koch Institut, Centre for Biological Safety, Nordufer 20, 13353 Berlin, Germany
Ann N Y Acad Sci 1078:563-5. 2006..burnetii. The numbers of IS1111 elements in the genomes of 75 C. burnetii isolates were quantified by real-time PCR and proved to be highly variable...
The genome of a Bacillus isolate causing anthrax in chimpanzees combines chromosomal properties of B. cereus with B. anthracis virulence plasmidsSilke R Klee
Centre for Biological Security ZBS, Robert Koch Institut, Berlin, Germany
PLoS ONE 5:e10986. 2010..cereus strain independently from the classic B. anthracis strains and established a B. anthracis lifestyle. Therefore we suggest to designate this isolate as "B. cereus variety (var.) anthracis"...
Development of antibodies against anthrose tetrasaccharide for specific detection of Bacillus anthracis sporesAndrea Kuehn
Robert Koch Institut, Nordufer 20, Berlin D 13353, Germany
Clin Vaccine Immunol 16:1728-37. 2009..Moreover, the antibodies generated proved functional in a new capture assay for B. anthracis spores and could therefore be useful for the detection of spores in complex samples...
Occurrence and genetic diversity of Bacillus anthracis strains isolated in an active wool-cleaning factoryPierre Wattiau
Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, Brussels, Belgium
Appl Environ Microbiol 74:4005-11. 2008..Goat hair originating from areas where anthrax is endemic remains a material with high biological risk for modern woolworkers...
