Michael Beekes

Summary

Affiliation: Robert Koch-Institut
Country: Germany

Publications

  1. ncbi request reprint Cerebral targeting indicates vagal spread of infection in hamsters fed with scrapie
    M Beekes
    Robert Koch Institut, Bundesinstitut für Infektionskrankheiten und nicht übertragbare Krankheiten, Berlin, Germany
    J Gen Virol 79:601-7. 1998
  2. ncbi request reprint The spread of prions through the body in naturally acquired transmissible spongiform encephalopathies
    Michael Beekes
    Robert Koch Institut P24 Transmissible Spongiforme Enzephalopathien, Berlin, Germany
    FEBS J 274:588-605. 2007
  3. ncbi request reprint Prion research: past, present, and future
    Michael Beekes
    P24, Transmissible Spongiform Encephalopathies, Robert Koch Institute Nordufer 20, 13353 Berlin, Germany
    Vet Microbiol 123:285-6. 2007
  4. ncbi request reprint Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion research
    Michael Beekes
    P24 Transmissible Spongiform Encephalopathies, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
    Vet Microbiol 123:305-19. 2007
  5. ncbi request reprint Aspects of risk assessment and risk management of nosocomial transmission of classical and variant Creutzfeldt-Jakob disease with special attention to German regulations
    Michael Beekes
    Robert Koch Institute, Berlin, Germany
    Contrib Microbiol 11:117-35. 2004
  6. doi request reprint [Variant Creutzfeld-Jakob disease (vCJD) : Epidemiology and prevention from human to human secondary transmission]
    M Beekes
    Transmissible Spongiforme Enzephalopathien, Robert Koch Institut, Berlin
    Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 53:597-605. 2010
  7. ncbi request reprint [Clinical aspects of risk assessment and prevention of nosocomial transmission of classical and variant CJD]
    M Beekes
    Robert Koch Institut P35, Nordufer 20, 13353 Berlin
    Internist (Berl) 43:738, 741-8. 2002
  8. doi request reprint Fast, broad-range disinfection of bacteria, fungi, viruses and prions
    Michael Beekes
    P24, Transmissible Spongiform Encephalopathies, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    J Gen Virol 91:580-9. 2010
  9. ncbi request reprint [BSE and Creutzfeldt-Jakob disease. Implication on health politics in Germany and Europe]
    M Beekes
    Robert Koch Institut, Berlin, Germany
    Dtsch Med Wochenschr 127:335-40. 2002
  10. ncbi request reprint Late increase of serum S100 beta protein levels in hamsters after oral or intraperitoneal infection with scrapie
    M Beekes
    Robert Koch Institut, 13353 Berlin, Germany
    J Infect Dis 180:518-20. 1999

Collaborators

Detail Information

Publications46

  1. ncbi request reprint Cerebral targeting indicates vagal spread of infection in hamsters fed with scrapie
    M Beekes
    Robert Koch Institut, Bundesinstitut für Infektionskrankheiten und nicht übertragbare Krankheiten, Berlin, Germany
    J Gen Virol 79:601-7. 1998
    ..For the first time, these results demonstrate conclusively that, in our animal model, initial infection of the brain after oral ingestion of scrapie agent occurs via the vagus nerve, rather than by spread along the spinal cord...
  2. ncbi request reprint The spread of prions through the body in naturally acquired transmissible spongiform encephalopathies
    Michael Beekes
    Robert Koch Institut P24 Transmissible Spongiforme Enzephalopathien, Berlin, Germany
    FEBS J 274:588-605. 2007
    ..It may also help to identify prophylactic or therapeutic approaches that would impede naturally acquired transmissible spongiform encephalopathy infections...
  3. ncbi request reprint Prion research: past, present, and future
    Michael Beekes
    P24, Transmissible Spongiform Encephalopathies, Robert Koch Institute Nordufer 20, 13353 Berlin, Germany
    Vet Microbiol 123:285-6. 2007
  4. ncbi request reprint Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion research
    Michael Beekes
    P24 Transmissible Spongiform Encephalopathies, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
    Vet Microbiol 123:305-19. 2007
    ..This article sets out to give a review of current examples of the analytical potential of FT-IR spectroscopy in microbiology and prion research...
  5. ncbi request reprint Aspects of risk assessment and risk management of nosocomial transmission of classical and variant Creutzfeldt-Jakob disease with special attention to German regulations
    Michael Beekes
    Robert Koch Institute, Berlin, Germany
    Contrib Microbiol 11:117-35. 2004
  6. doi request reprint [Variant Creutzfeld-Jakob disease (vCJD) : Epidemiology and prevention from human to human secondary transmission]
    M Beekes
    Transmissible Spongiforme Enzephalopathien, Robert Koch Institut, Berlin
    Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 53:597-605. 2010
    ..This article provides an overview of the surveillance and epidemiology of vCJD and outlines public health strategies for the risk assessment and risk management of this novel BSE-associated prion disease in humans...
  7. ncbi request reprint [Clinical aspects of risk assessment and prevention of nosocomial transmission of classical and variant CJD]
    M Beekes
    Robert Koch Institut P35, Nordufer 20, 13353 Berlin
    Internist (Berl) 43:738, 741-8. 2002
  8. doi request reprint Fast, broad-range disinfection of bacteria, fungi, viruses and prions
    Michael Beekes
    P24, Transmissible Spongiform Encephalopathies, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    J Gen Virol 91:580-9. 2010
    ..The formulation was not found to fix proteins more than was observed with water as the cleaning reagent. In conclusion, SDS, NaOH and n-propanol can synergistically achieve fast, broad-range disinfection...
  9. ncbi request reprint [BSE and Creutzfeldt-Jakob disease. Implication on health politics in Germany and Europe]
    M Beekes
    Robert Koch Institut, Berlin, Germany
    Dtsch Med Wochenschr 127:335-40. 2002
  10. ncbi request reprint Late increase of serum S100 beta protein levels in hamsters after oral or intraperitoneal infection with scrapie
    M Beekes
    Robert Koch Institut, 13353 Berlin, Germany
    J Infect Dis 180:518-20. 1999
    ....
  11. ncbi request reprint Early accumulation of pathological PrP in the enteric nervous system and gut-associated lymphoid tissue of hamsters orally infected with scrapie
    M Beekes
    Robert Koch lnstitut P31, Berlin, Germany
    Neurosci Lett 278:181-4. 2000
    ..These results provide evidence for an important role of the ENS in scrapie neuroinvasion and for centripetal vagal spread of infection from the gut to the brain after oral uptake of agent...
  12. ncbi request reprint Decontamination of surgical instruments from prions. II. In vivo findings with a model system for testing the removal of scrapie infectivity from steel surfaces
    Karin Lemmer
    P24, Transmissible Spongiform Encephalopathies, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    J Gen Virol 89:348-58. 2008
    ..Methods identified as highly effective in the 263K steel wire model need to be validated for human TSE agents on different types of instrument surfaces...
  13. pmc Changes in protein structure and distribution observed at pre-clinical stages of scrapie pathogenesis
    Ariane Kretlow
    P25, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Biochim Biophys Acta 1782:559-65. 2008
    ....
  14. ncbi request reprint Pathological prion protein in muscles of hamsters and mice infected with rodent-adapted BSE or vCJD
    Achim Thomzig
    Robert Koch Institut P24 Transmissible Spongiform Encephalopathies, Berlin, Germany
    J Gen Virol 87:251-4. 2006
    ..Our findings emphasize the need for further assessment of possible public-health risks from TSE involvement of skeletal muscle...
  15. ncbi request reprint Discriminating scrapie and bovine spongiform encephalopathy isolates by infrared spectroscopy of pathological prion protein
    Achim Thomzig
    Robert Koch Institut, P26 and P13, Nordufer 20, 13353 Berlin, Germany
    J Biol Chem 279:33847-54. 2004
    ....
  16. ncbi request reprint In situ identification of protein structural changes in prion-infected tissue
    Janina Kneipp
    Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Biochim Biophys Acta 1639:152-8. 2003
    ..No evidence of these structural changes was observed in normal neurons...
  17. ncbi request reprint Structural differences between TSEs strains investigated by FT-IR spectroscopy
    Sashko Spassov
    P25, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Biochim Biophys Acta 1760:1138-49. 2006
    ....
  18. pmc Preclinical deposition of pathological prion protein PrPSc in muscles of hamsters orally exposed to scrapie
    Achim Thomzig
    Robert Koch Institut, P26 Pathogenese und Diagnostik Transmissibler Spongiformer Enzephalopathien, Berlin, Germany
    J Clin Invest 113:1465-72. 2004
    ....
  19. pmc Quantitative detection and biological propagation of scrapie seeding activity in vitro facilitate use of prions as model pathogens for disinfection
    Sandra Pritzkow
    Transmissible Spongiform Encephalopathies, Project Group Immune Defense and Pathogenesis, Robert Koch Institut, Berlin, Germany
    PLoS ONE 6:e20384. 2011
    ....
  20. ncbi request reprint FTIR-microspectroscopy of prion-infected nervous tissue
    Ariane Kretlow
    P25, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Biochim Biophys Acta 1758:948-59. 2006
    ....
  21. pmc Accumulation of pathological prion protein PrPSc in the skin of animals with experimental and natural scrapie
    Achim Thomzig
    P24 Transmissible Spongiform Encephalopathies, Robert Koch Institut, Berlin, Germany
    PLoS Pathog 3:e66. 2007
    ..Our findings point to the skin as a potential reservoir of prions, which should be further investigated in relation to disease transmission...
  22. ncbi request reprint Prion propagation in a nerve conduit model containing segments devoid of axons
    Christine Kratzel
    Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
    J Gen Virol 88:3479-85. 2007
    ....
  23. ncbi request reprint Molecular changes of preclinical scrapie can be detected by infrared spectroscopy
    Janina Kneipp
    PG3, Robert Koch Institut, D 13353 Berlin, Germany
    J Neurosci 22:2989-97. 2002
    ....
  24. pmc Widespread PrPSc accumulation in muscles of hamsters orally infected with scrapie
    Achim Thomzig
    Robert Koch Institut, Berlin, Germany
    EMBO Rep 4:530-3. 2003
    ..Therefore, the findings described here highlight further the necessity to investigate thoroughly whether muscles of TSE-infected sheep, cattle, elk and deer contain infectious agents...
  25. pmc Harnessing prions as test agents for the development of broad-range disinfectants
    Katja Wagenführ
    P24 Transmissible Spongiform Encephalopathies, Robert Koch Institut, Berlin, Germany
    Prion 6:1-6. 2012
    ..Our approach will substantially facilitate the identification of disinfectants with efficacy against prions as promising candidates for a further microbiological validation of broad-range activity...
  26. pmc Relevance of the regional lymph node in scrapie pathogenesis after peripheral infection of hamsters
    Christine Kratzel
    Robert Koch Institut, P24 Transmissible Spongiforme Enzephalopathien Nordufer 20, D Berlin 13353, Germany
    BMC Vet Res 3:22. 2007
    ..Against this background, the influence of the regional lymph node (Ln. popliteus) on the pathogenesis of scrapie was monitored in a hamster model of prion infection via the footpad...
  27. pmc Faecal shedding, alimentary clearance and intestinal spread of prions in hamsters fed with scrapie
    Dominique Krüger
    Robert Koch Institute, P24 Transmissible Spongiform Encephalopathies, Nordufer 20, D 13353 Berlin, Germany
    Vet Res 40:4. 2009
    ..Although excrement may thus provide a vehicle for the release of endogenously formed PrP(TSE), intestinal clearance mechanisms seem to partially counteract such a mode of prion dissemination...
  28. ncbi request reprint Detection of preclinical scrapie from serum by infrared spectroscopy and chemometrics
    Peter Lasch
    P25 Biomedical Spectroscopy, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Anal Bioanal Chem 387:1791-800. 2007
    ..Possible spectral markers responsible for the discrimination capacity of the two different ANNs are discussed...
  29. ncbi request reprint Propagation of scrapie in peripheral nerves after footpad infection in normal and neurotoxin exposed hamsters
    Christine Kratzel
    Robert Koch Institut, P24 Transmissible Spongiforme Enzephalopathien, Nordufer 20, 13353 Berlin, Germany
    Vet Res 38:127-39. 2007
    ..Additionally, the model indicates that the spread of PrP(Sc) is not mediated by the slow component ;a' of the axonal transport mechanism...
  30. ncbi request reprint Antemortem identification of bovine spongiform encephalopathy from serum using infrared spectroscopy
    Peter Lasch
    P13, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Anal Chem 75:6673-8. 2003
    ..The combination of classification algorithms increased the sensitivity and specificity of BSE detection to 96 and 92%, respectively...
  31. doi request reprint Towards further reduction and replacement of animal bioassays in prion research by cell and protein misfolding cyclic amplification assays
    Susann Boerner
    Work Group Unconventional Pathogens and Their Inactivation, Division of Applied Infection Control and Hospital Hygiene, Department of Infectious Diseases, Robert Koch Institut, 13353 Berlin, Germany
    Lab Anim 47:106-15. 2013
    ..Our rationale and methodology can be systematically extended to other types of prions and used to further reduce or replace prion bioassays in rodents...
  32. ncbi request reprint Decontamination of surgical instruments from prion proteins: in vitro studies on the detachment, destabilization and degradation of PrPSc bound to steel surfaces
    Karin Lemmer
    P24 Transmissible Spongiforme Enzephalopathien, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    J Gen Virol 85:3805-16. 2004
    ..9) or 5 % SDS (pH 7.1) exert potent decontaminating activities on PrP(Sc)/PrP27-30 attached to steel surfaces. For in vivo validation, wires reprocessed in these reagents have been implanted into reporter animals in ongoing experiments...
  33. ncbi request reprint [Inactivation and removal of prions in producing medical products. A contribution to evaluation and declaration of possible methods]
    J Bertram
    Robert Koch Institut, Berlin
    Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 47:36-40. 2004
  34. ncbi request reprint Detection of pathological molecular alterations in scrapie-infected hamster brain by Fourier transform infrared (FT-IR) spectroscopy
    J Kneipp
    PG3, Robert Koch Institut, Nordufer 20, D 13353, Berlin, Germany
    Biochim Biophys Acta 1501:189-99. 2000
    ..This method provides a useful tool not only for the exploration of the disease process but also for the development of rapid diagnostic and screening techniques of transmissible spongiform encephalopathies...
  35. ncbi request reprint Prions and prion diseases
    Michael Beekes
    Robert Koch Institut P24 Transmissible Spongiforme Enzephalopathien, Berlin, Germany
    FEBS J 274:575. 2007
  36. ncbi request reprint Scrapie-infected cells, isolated prions, and recombinant prion protein: a comparative study
    J Kneipp
    Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
    Biopolymers 74:163-7. 2004
    ....
  37. pmc Chronic wasting disease: fingerprinting the culprit in risk assessments
    Martin L Daus
    P24 Transmissible Spongiform Encephalopathies, Robert Koch Institut, Berlin, Germany
    Prion 6:17-22. 2012
    ..CWD typing by Fourier transform-infrared spectroscopy of pathological prion protein may provide a method of choice for this purpose...
  38. pmc Presence and seeding activity of pathological prion protein (PrP(TSE)) in skeletal muscles of white-tailed deer infected with chronic wasting disease
    Martin L Daus
    P24 Transmissible Spongiform Encephalopathies, Robert Koch Institut, Berlin, Germany
    PLoS ONE 6:e18345. 2011
    ....
  39. ncbi request reprint Generation of genuine prion infectivity by serial PMCA
    Petra Weber
    Centre for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 23, 81377 Munich, Germany
    Vet Microbiol 123:346-57. 2007
    ....
  40. ncbi request reprint Detection of prion particles in samples of BSE and scrapie by fluorescence correlation spectroscopy without proteinase K digestion
    Eva Birkmann
    Heinrich Heine Universitat Dusseldorf, Institut für Physikalische Biologie, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Biol Chem 387:95-102. 2006
    ..Thus, a diagnostic tool for BSE detection with complete avoidance of PK treatment is presented, which should have particular advantages for testing animals in the preclinical stage...
  41. ncbi request reprint Photo-induced crosslinking of prion protein oligomers and prions
    Niklas Piening
    Zentrum für Neuropathologie und Prionforschung, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 23, 81377 Munchen, Germany
    Amyloid 13:67-77. 2006
    ....
  42. pmc Cell-free formation of misfolded prion protein with authentic prion infectivity
    Petra Weber
    Center for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 23, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 103:15818-23. 2006
    ....
  43. ncbi request reprint Identification of scrapie infection from blood serum by Fourier transform infrared spectroscopy
    Jürgen Schmitt
    FB VI, Spectroscopy Group, University of Trier, Universitätsring, Germany
    Anal Chem 74:3865-8. 2002
    ..The predictive value of a positive (negative) test was 100% (98%). The proposed serum test circumvents substantial drawbacks of conventional TSE diagnostics and can be fully automated...
  44. pmc Autocatalytic self-propagation of misfolded prion protein
    Jan Bieschke
    Center for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 23, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 101:12207-11. 2004
    ..PrPres formation was not paralleled by replication of biological infectivity, which appears to require factors additional to PrP-misfolding autocatalysis...
  45. ncbi request reprint The polysaccharide scaffold of PrP 27-30 is a common compound of natural prions and consists of alpha-linked polyglucose
    Christian Dumpitak
    , , , Germany
    Biol Chem 386:1149-55. 2005
    ..The origin of the polysaccharide, its interaction with PrP and its potential relation to glycogen and corpora amylacea are discussed...
  46. ncbi request reprint Acid inactivation of prions: efficient at elevated temperature or high acid concentration
    Thomas R Appel
    IMB, Leibniz Institut für Molekulare Biotechnologie Jena eV, Germany
    J Gen Virol 87:1385-94. 2006
    ..Most importantly, infectivity was always inactivated faster or to a higher extent than PrP was hydrolysed at several incubation times, HCl concentrations and temperatures...