Research Topics
| Michael BeekesSummaryAffiliation: Robert Koch-Institut Country: Germany Publications
| Collaborators
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Detail Information
Publications
Cerebral targeting indicates vagal spread of infection in hamsters fed with scrapieM Beekes
Robert Koch Institut, Bundesinstitut für Infektionskrankheiten und nicht übertragbare Krankheiten, Berlin, Germany
J Gen Virol 79:601-7. 1998..For the first time, these results demonstrate conclusively that, in our animal model, initial infection of the brain after oral ingestion of scrapie agent occurs via the vagus nerve, rather than by spread along the spinal cord...- The spread of prions through the body in naturally acquired transmissible spongiform encephalopathiesMichael Beekes
Robert Koch Institut P24 Transmissible Spongiforme Enzephalopathien, Berlin, Germany
FEBS J 274:588-605. 2007..It may also help to identify prophylactic or therapeutic approaches that would impede naturally acquired transmissible spongiform encephalopathy infections... - Prion research: past, present, and futureMichael Beekes
P24, Transmissible Spongiform Encephalopathies, Robert-Koch-Institute Nordufer 20, 13353 Berlin, Germany
Vet Microbiol 123:285-6. 2007 - Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion researchMichael Beekes
P24 Transmissible Spongiform Encephalopathies, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Vet Microbiol 123:305-19. 2007..This article sets out to give a review of current examples of the analytical potential of FT-IR spectroscopy in microbiology and prion research... - Aspects of risk assessment and risk management of nosocomial transmission of classical and variant Creutzfeldt-Jakob disease with special attention to German regulationsMichael Beekes
Robert Koch Institute, Berlin, Germany
Contrib Microbiol 11:117-35. 2004 
[Variant Creutzfeld-Jakob disease (vCJD) : Epidemiology and prevention from human to human secondary transmission]M Beekes
Transmissible Spongiforme Enzephalopathien, Robert Koch Institut, Berlin
Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 53:597-605. 2010..This article provides an overview of the surveillance and epidemiology of vCJD and outlines public health strategies for the risk assessment and risk management of this novel BSE-associated prion disease in humans...- [Clinical aspects of risk assessment and prevention of nosocomial transmission of classical and variant CJD]M Beekes
Robert Koch-Institut (P35, Nordufer 20, 13353 Berlin
Internist (Berl) 43:738, 741-8. 2002 - Fast, broad-range disinfection of bacteria, fungi, viruses and prionsMichael Beekes
P24, Transmissible Spongiform Encephalopathies, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
J Gen Virol 91:580-9. 2010..The formulation was not found to fix proteins more than was observed with water as the cleaning reagent. In conclusion, SDS, NaOH and n-propanol can synergistically achieve fast, broad-range disinfection... - [BSE and Creutzfeldt-Jakob disease. Implication on health politics in Germany and Europe]M Beekes
Robert Koch-Institut, Berlin, Germany
Dtsch Med Wochenschr 127:335-40. 2002 - Late increase of serum S100 beta protein levels in hamsters after oral or intraperitoneal infection with scrapieM Beekes
Robert Koch Institut, 13353 Berlin, Germany
J Infect Dis 180:518-20. 1999.... - Early accumulation of pathological PrP in the enteric nervous system and gut-associated lymphoid tissue of hamsters orally infected with scrapieM Beekes
Robert Koch lnstitut P31, Berlin, Germany
Neurosci Lett 278:181-4. 2000..These results provide evidence for an important role of the ENS in scrapie neuroinvasion and for centripetal vagal spread of infection from the gut to the brain after oral uptake of agent... - Decontamination of surgical instruments from prions. II. In vivo findings with a model system for testing the removal of scrapie infectivity from steel surfacesKarin Lemmer
P24, Transmissible Spongiform Encephalopathies, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
J Gen Virol 89:348-58. 2008..Methods identified as highly effective in the 263K steel wire model need to be validated for human TSE agents on different types of instrument surfaces... 
Changes in protein structure and distribution observed at pre-clinical stages of scrapie pathogenesisAriane Kretlow
P25, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
Biochim Biophys Acta 1782:559-65. 2008....- Pathological prion protein in muscles of hamsters and mice infected with rodent-adapted BSE or vCJDAchim Thomzig
Robert Koch-Institut (P24 - Transmissible Spongiform Encephalopathies, Berlin, Germany
J Gen Virol 87:251-4. 2006..Our findings emphasize the need for further assessment of possible public-health risks from TSE involvement of skeletal muscle... - Discriminating scrapie and bovine spongiform encephalopathy isolates by infrared spectroscopy of pathological prion proteinAchim Thomzig
Robert Koch Institut, P26 and P13, Nordufer 20, 13353 Berlin, Germany
J Biol Chem 279:33847-54. 2004.... - In situ identification of protein structural changes in prion-infected tissueJanina Kneipp
Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
Biochim Biophys Acta 1639:152-8. 2003..No evidence of these structural changes was observed in normal neurons... - Structural differences between TSEs strains investigated by FT-IR spectroscopySashko Spassov
P25, Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany
Biochim Biophys Acta 1760:1138-49. 2006.... - Preclinical deposition of pathological prion protein PrPSc in muscles of hamsters orally exposed to scrapieAchim Thomzig
Robert Koch-Institut, P26--Pathogenese und Diagnostik Transmissibler Spongiformer Enzephalopathien, Berlin, Germany
J Clin Invest 113:1465-72. 2004.... - Quantitative detection and biological propagation of scrapie seeding activity in vitro facilitate use of prions as model pathogens for disinfectionSandra Pritzkow
Transmissible Spongiform Encephalopathies, Project Group Immune Defense and Pathogenesis, Robert Koch Institut, Berlin, Germany
PLoS ONE 6:e20384. 2011.... - FTIR-microspectroscopy of prion-infected nervous tissueAriane Kretlow
P25, Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany
Biochim Biophys Acta 1758:948-59. 2006.... - Accumulation of pathological prion protein PrPSc in the skin of animals with experimental and natural scrapieAchim Thomzig
P24 Transmissible Spongiform Encephalopathies, Robert Koch Institut, Berlin, Germany
PLoS Pathog 3:e66. 2007..Our findings point to the skin as a potential reservoir of prions, which should be further investigated in relation to disease transmission... - Prion propagation in a nerve conduit model containing segments devoid of axonsChristine Kratzel
Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
J Gen Virol 88:3479-85. 2007.... - Molecular changes of preclinical scrapie can be detected by infrared spectroscopyJanina Kneipp
PG3, Robert Koch Institut, D 13353 Berlin, Germany
J Neurosci 22:2989-97. 2002.... - Widespread PrPSc accumulation in muscles of hamsters orally infected with scrapieAchim Thomzig
Robert Koch-Institut, Berlin, Germany
EMBO Rep 4:530-3. 2003..Therefore, the findings described here highlight further the necessity to investigate thoroughly whether muscles of TSE-infected sheep, cattle, elk and deer contain infectious agents... - Harnessing prions as test agents for the development of broad-range disinfectantsKatja Wagenführ
P24 Transmissible Spongiform Encephalopathies, Robert Koch Institut, Berlin, Germany
Prion 6:1-6. 2012..Our approach will substantially facilitate the identification of disinfectants with efficacy against prions as promising candidates for a further microbiological validation of broad-range activity... - Relevance of the regional lymph node in scrapie pathogenesis after peripheral infection of hamstersChristine Kratzel
Robert Koch Institut, P24 Transmissible Spongiforme Enzephalopathien Nordufer 20, D Berlin 13353, Germany
BMC Vet Res 3:22. 2007..Against this background, the influence of the regional lymph node (Ln. popliteus) on the pathogenesis of scrapie was monitored in a hamster model of prion infection via the footpad... - Faecal shedding, alimentary clearance and intestinal spread of prions in hamsters fed with scrapieDominique Krüger
Robert Koch Institute, P24 Transmissible Spongiform Encephalopathies, Nordufer 20, D 13353 Berlin, Germany
Vet Res 40:4. 2009..Although excrement may thus provide a vehicle for the release of endogenously formed PrP(TSE), intestinal clearance mechanisms seem to partially counteract such a mode of prion dissemination... - Detection of preclinical scrapie from serum by infrared spectroscopy and chemometricsPeter Lasch
P25 Biomedical Spectroscopy, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
Anal Bioanal Chem 387:1791-800. 2007..Possible spectral markers responsible for the discrimination capacity of the two different ANNs are discussed... - Antemortem identification of bovine spongiform encephalopathy from serum using infrared spectroscopyPeter Lasch
P13, Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany
Anal Chem 75:6673-8. 2003..The combination of classification algorithms increased the sensitivity and specificity of BSE detection to 96 and 92%, respectively... - Towards further reduction and replacement of animal bioassays in prion research by cell and protein misfolding cyclic amplification assaysSusann Boerner
Work Group Unconventional Pathogens and Their Inactivation, Division of Applied Infection Control and Hospital Hygiene, Department of Infectious Diseases, Robert Koch Institut, 13353 Berlin, Germany
Lab Anim 47:106-15. 2013..Our rationale and methodology can be systematically extended to other types of prions and used to further reduce or replace prion bioassays in rodents... - Decontamination of surgical instruments from prion proteins: in vitro studies on the detachment, destabilization and degradation of PrPSc bound to steel surfacesKarin Lemmer
P24 Transmissible Spongiforme Enzephalopathien, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
J Gen Virol 85:3805-16. 2004..9) or 5 % SDS (pH 7.1) exert potent decontaminating activities on PrP(Sc)/PrP27-30 attached to steel surfaces. For in vivo validation, wires reprocessed in these reagents have been implanted into reporter animals in ongoing experiments... - Propagation of scrapie in peripheral nerves after footpad infection in normal and neurotoxin exposed hamstersChristine Kratzel
Robert Koch Institut, P24 Transmissible Spongiforme Enzephalopathien, Nordufer 20, 13353 Berlin, Germany
Vet Res 38:127-39. 2007..Additionally, the model indicates that the spread of PrP(Sc) is not mediated by the slow component ;a' of the axonal transport mechanism... - [Inactivation and removal of prions in producing medical products. A contribution to evaluation and declaration of possible methods]J Bertram
Robert Koch-Institut, Berlin
Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 47:36-40. 2004 - Detection of pathological molecular alterations in scrapie-infected hamster brain by Fourier transform infrared (FT-IR) spectroscopyJ Kneipp
PG3, Robert Koch Institut, Nordufer 20, D 13353, Berlin, Germany
Biochim Biophys Acta 1501:189-99. 2000..This method provides a useful tool not only for the exploration of the disease process but also for the development of rapid diagnostic and screening techniques of transmissible spongiform encephalopathies... - Prions and prion diseasesMichael Beekes
Robert Koch-Institut (P24 - Transmissible Spongiforme Enzephalopathien, Berlin, Germany
FEBS J 274:575. 2007 - Scrapie-infected cells, isolated prions, and recombinant prion protein: a comparative studyJ Kneipp
Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Biopolymers 74:163-7. 2004.... - Chronic wasting disease: fingerprinting the culprit in risk assessmentsMartin L Daus
P24 Transmissible Spongiform Encephalopathies, Robert Koch Institut, Berlin, Germany
Prion 6:17-22. 2012..CWD typing by Fourier transform-infrared spectroscopy of pathological prion protein may provide a method of choice for this purpose... - Presence and seeding activity of pathological prion protein (PrP(TSE)) in skeletal muscles of white-tailed deer infected with chronic wasting diseaseMartin L Daus
P24 Transmissible Spongiform Encephalopathies, Robert Koch Institut, Berlin, Germany
PLoS ONE 6:e18345. 2011.... - Detection of prion particles in samples of BSE and scrapie by fluorescence correlation spectroscopy without proteinase K digestionEva Birkmann
, , , , Germany
Biol Chem 387:95-102. 2006..Thus, a diagnostic tool for BSE detection with complete avoidance of PK treatment is presented, which should have particular advantages for testing animals in the preclinical stage... - Autocatalytic self-propagation of misfolded prion proteinJan Bieschke
Center for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 23, 81377 Munich, Germany
Proc Natl Acad Sci U S A 101:12207-11. 2004..PrPres formation was not paralleled by replication of biological infectivity, which appears to require factors additional to PrP-misfolding autocatalysis... - Photo-induced crosslinking of prion protein oligomers and prionsNiklas Piening
, , Feodor-Lynen-Strasse 23, , Germany
Amyloid 13:67-77. 2006.... - Cell-free formation of misfolded prion protein with authentic prion infectivityPetra Weber
Center for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor-Lynen-Strasse 23, 81377 Munich, Germany
Proc Natl Acad Sci U S A 103:15818-23. 2006.... - Identification of scrapie infection from blood serum by Fourier transform infrared spectroscopyJürgen Schmitt
FB VI, Spectroscopy Group, University of Trier, Universitätsring, Germany
Anal Chem 74:3865-8. 2002..The predictive value of a positive (negative) test was 100% (98%). The proposed serum test circumvents substantial drawbacks of conventional TSE diagnostics and can be fully automated... - Generation of genuine prion infectivity by serial PMCAPetra Weber
Centre for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 23, 81377 Munich, Germany
Vet Microbiol 123:346-57. 2007.... - The polysaccharide scaffold of PrP 27-30 is a common compound of natural prions and consists of alpha-linked polyglucoseChristian Dumpitak
, , , Germany
Biol Chem 386:1149-55. 2005..The origin of the polysaccharide, its interaction with PrP and its potential relation to glycogen and corpora amylacea are discussed... - Acid inactivation of prions: efficient at elevated temperature or high acid concentrationThomas R Appel
IMB, , Germany
J Gen Virol 87:1385-94. 2006..Most importantly, infectivity was always inactivated faster or to a higher extent than PrP was hydrolysed at several incubation times, HCl concentrations and temperatures...