D Rath

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..Such a test is needed, especially for "custom sorting" programs. Currently, test sorts are the only means of obtaining information about the sorting efficiency of semen from a particular bull...

..Recent developments in ovine AI and ET will make it very likely that commercial sheep industry will adopt the sexing technology in their breeding concepts...

..With this combination of different approaches, acceptable fertility with cryopreserved boar semen can be achieved, facilitating the use of cryopreserved boar semen in routine AI programs...

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..5%). Additionally, it was shown for the first time that artificial insemination (AI) with liquid sexed bull spermatozoa stored for 72 h after sorting can result in pregnancy rates similar to AI with non-sorted semen...

..05). In conclusion, based on the present results, addition of AOs to cooling and freezing extenders and/or replacement of GLY with DMF or DMA could not improve quality of frozen-thawed boar sperm...

..3 +/- 10.0%). This study demonstrates the highly protective effects of antioxidants on the quality of flow cytometrically sorted frozen-thawed bull spermatozoa...

..In combination with the increasing knowledge in gene sequences this will allow in the foreseeable future widespread use in the pig industry either for agricultural or biomedical purposes...

..5%. Results show that this semen preservation method is robust and efficient and associated with high fertility. It may also be useful for other animal species...

..The results of the study suggest that viable spermatozoa are already subject to selective processes within the uterus before further selection is initiated at the utero-tubal junction and in the oviductal isthmus...

..1% for male and 38.9% for female sex. It is concluded that non-surgically inseminations with flowcytometrically sexed spermatozoa can be conducted successfully...

..It is recommended that future research focuses more on the exact mechanism behind the observed effects, because such information would facilitate the production of nanoparticles with increased biocompatibility...

..This is the first characterisation of spermatozoa from a pig cohort carrying a targeted cassette exchange. This large animal model may help to elucidate the function of paternally transmitted components to fertilized oocytes...

..Major advances have been registered, which have improved cryo-survival and the capacity to process boar semen for commercial AI...

..Altogether, the improvements made in the recent years will allow a broader use of sex-sorted spermatozoa even in those species that require more cells than cows and sheep...

..Further studies involving gametes and embryos of large animal models are ongoing to intensify our understanding of silver nanoparticle reprotoxicity...

..In conclusion, the biocompatibility of gold nanoparticles depends on a range of particle specific aspects as well as the choice of target tissue. Further clarification of such matters are subject to ongoing research...

..Sleeping Beauty-mediated transposition is thus a promising approach for genetic modification of the pig genome...

..Hatched or micro-manipulated embryos may increase the risk of transmission of CSFV by embryo transfer, which has to be confirmed by in vivo tests under isolation conditions...

..With respect to the fertilizing capacities of their ejaculates, boars have to be selected more strictly for sperm quality parameters as most of the compensatory effects of sperm cells disappear in maximally extended semen samples...

..A functional nuclear transfer system will be crucial for xenotransplantation as it is anticipated that the expression of several transgenes will be required...

..Investigations for further confirmation of the suggested binding mechanism between porcine sperm and the uterine epithelial layer are ongoing...