QIAGEN GmbH

Summary

Organization: QIAGEN GmbH
Country: Germany

Top Publications

  1. ncbi Automated sample-preparation technologies in genome sequencing projects
    H Hilbert
    QIAGEN GmbH, Hilden, Germany
    DNA Seq 11:193-7. 2000
  2. pmc HPV genotype detection using hybrid capture sample preparation combined with whole genome amplification and multiplex detection with Luminex XMAP
    Brian Lowe
    QIAGEN, Inc, Research and Development, 1201 Clopper Road, Gaithersburg, MD 20878, USA
    J Mol Diagn 12:847-53. 2010
  3. doi Differential expression proteomics of human colorectal cancer based on a syngeneic cellular model for the progression of adenoma to carcinoma
    Udo Roth
    Center for Molecular Medicine Cologne CMMC, University of Cologne, 50931 Koln, Germany
    Proteomics 10:194-202. 2010
  4. pmc Optimized quantification of fragmented, free circulating DNA in human blood plasma using a calibrated duplex real-time PCR
    Martin Horlitz
    QIAGEN GmbH, R and D Department, Hilden, Germany
    PLoS ONE 4:e7207. 2009
  5. ncbi Expression of 25-hydroxyvitamin D-1alpha-hydroxylase (1alphaOHase, CYP27B1) splice variants in HaCaT keratinocytes and other skin cells: modulation by culture conditions and UV-B treatment in vitro
    Markus Seifert
    Universitäts Hautklinik, Universitatsklinikum des Saarlandes, Homburg, Germany
    Anticancer Res 29:3659-67. 2009
  6. doi The next-generation Hybrid Capture High-Risk HPV DNA assay on a fully automated platform
    Paul S Eder
    QIAGEN, Inc, Gaithersburg, MD, USA
    J Clin Virol 45:S85-92. 2009
  7. pmc A highly specific system for efficient enzymatic removal of tags from recombinant proteins
    Frank Schafer
    QIAGEN GmbH, Hilden, Germany
    J Biomol Tech 13:158-71. 2002
  8. pmc Automated high-throughput purification of 6xHis-tagged proteins
    Frank Schafer
    QIAGEN GmbH, Hilden, Germany
    J Biomol Tech 13:131-42. 2002
  9. doi A novel method of HPV genotyping using Hybrid Capture sample preparation method combined with GP5+/6+ PCR and multiplex detection on Luminex XMAP
    I Nazarenko
    QIAGEN Gaithersburg, Inc, 1201 Clopper Road, Gaithersburg, MD 20878, USA
    J Virol Methods 154:76-81. 2008
  10. pmc Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
    Jennifer Owen
    Institute of Evolutionary Biology, University of Edinburgh, King s Buildings, Edinburgh, EH9 3JT, UK
    BMC Genomics 9:266. 2008

Scientific Experts

Detail Information

Publications15

  1. ncbi Automated sample-preparation technologies in genome sequencing projects
    H Hilbert
    QIAGEN GmbH, Hilden, Germany
    DNA Seq 11:193-7. 2000
    ....
  2. pmc HPV genotype detection using hybrid capture sample preparation combined with whole genome amplification and multiplex detection with Luminex XMAP
    Brian Lowe
    QIAGEN, Inc, Research and Development, 1201 Clopper Road, Gaithersburg, MD 20878, USA
    J Mol Diagn 12:847-53. 2010
    ..72) with both a PCR-based HPV genotyping assay (n = 131) and the HC2 assay (n = 502) using representative cervical specimens. This assay may be easy to automate and could be applied for the detection of other targets in future studies...
  3. doi Differential expression proteomics of human colorectal cancer based on a syngeneic cellular model for the progression of adenoma to carcinoma
    Udo Roth
    Center for Molecular Medicine Cologne CMMC, University of Cologne, 50931 Koln, Germany
    Proteomics 10:194-202. 2010
    ....
  4. pmc Optimized quantification of fragmented, free circulating DNA in human blood plasma using a calibrated duplex real-time PCR
    Martin Horlitz
    QIAGEN GmbH, R and D Department, Hilden, Germany
    PLoS ONE 4:e7207. 2009
    ..This leads to inaccuracies when counting PCR target copy numbers as whole genome equivalents...
  5. ncbi Expression of 25-hydroxyvitamin D-1alpha-hydroxylase (1alphaOHase, CYP27B1) splice variants in HaCaT keratinocytes and other skin cells: modulation by culture conditions and UV-B treatment in vitro
    Markus Seifert
    Universitäts Hautklinik, Universitatsklinikum des Saarlandes, Homburg, Germany
    Anticancer Res 29:3659-67. 2009
    ..Further study of the impact of CYP27B1 splice variants on the vitamin D pathway in keratinocytes and other cell types is warranted...
  6. doi The next-generation Hybrid Capture High-Risk HPV DNA assay on a fully automated platform
    Paul S Eder
    QIAGEN, Inc, Gaithersburg, MD, USA
    J Clin Virol 45:S85-92. 2009
    ....
  7. pmc A highly specific system for efficient enzymatic removal of tags from recombinant proteins
    Frank Schafer
    QIAGEN GmbH, Hilden, Germany
    J Biomol Tech 13:158-71. 2002
    ..For cleavage of both 6xHis-tagged GFP and IL-1beta, DAPase enzyme gave an average cleavage rate of 1.5 +/- 0.5 min per amino acid...
  8. pmc Automated high-throughput purification of 6xHis-tagged proteins
    Frank Schafer
    QIAGEN GmbH, Hilden, Germany
    J Biomol Tech 13:131-42. 2002
    ..coli), in less than 3 h. Examples of purification and assay applications using the protocols mentioned above, and data on reproducibility and cross-contamination-free processing are shown...
  9. doi A novel method of HPV genotyping using Hybrid Capture sample preparation method combined with GP5+/6+ PCR and multiplex detection on Luminex XMAP
    I Nazarenko
    QIAGEN Gaithersburg, Inc, 1201 Clopper Road, Gaithersburg, MD 20878, USA
    J Virol Methods 154:76-81. 2008
    ..The clinical utility of this method was demonstrated on cervical samples positive for HR HPV by the Hybrid Capture 2 (hc2) screening assay...
  10. pmc Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
    Jennifer Owen
    Institute of Evolutionary Biology, University of Edinburgh, King s Buildings, Edinburgh, EH9 3JT, UK
    BMC Genomics 9:266. 2008
    ..The clitellate annelid Lumbricus rubellus is a model organism for soil health testing, but genetic data have been lacking...
  11. pmc Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail
    Salim Essakali
    Department of Pathology, Peter MacCallum Cancer Centre, St Andrews Place, Melbourne, Victoria 3002, Australia
    BMC Biotechnol 8:6. 2008
    ..High purity of tumour samples is a necessity for accurate genetic and expression analysis and is usually achieved by positive selection in chronic lymphocytic leukaemia (CLL)...
  12. ncbi Insights into effective RNAi gained from large-scale siRNA validation screening
    Ute Krueger
    QIAGEN GmbH, Hilden, Germany
    Oligonucleotides 17:237-50. 2007
    ....
  13. ncbi Using aptamers as capture reagents in bead-based assay systems for diagnostics and hit identification
    Peter Porschewski
    QIAGEN GmbH, Hilden, Germany
    J Biomol Screen 11:773-81. 2006
    ..These methods should be transferable to a large number of applications because specific aptamers can be rapidly generated for almost any protein target...
  14. ncbi Gene transfer into eukaryotic cells using activated polyamidoamine dendrimers
    Jörg Dennig
    QIAGEN GmbH, Hilden, Germany
    J Biotechnol 90:339-47. 2002
    ..This technology could also be useful for in vivo gene transfer in gene therapy applications...
  15. ncbi Gene transfer in eukaryotic cells using activated dendrimers
    Jörg Dennig
    QIAGEN GmbH, Hilden, Max Volmer Strasse 4, 40724, Germany
    Top Curr Chem 228:227-36. 2003
    ..A structural model of the activated dendrimer-DNA complex and a potential mechanism for its uptake into cells will be discussed...