T W Stief

Summary

Affiliation: Philipps University
Country: Germany

Publications

  1. ncbi request reprint Determination of the anti-F10a or anti-F2a generation action of rivaroxaban or dabigatran
    Thomas W Stief
    Institute of Laboratory Medicine and Pathobiochemistry, University Hospital, Marburg, Germany
    Blood Coagul Fibrinolysis 23:619-21. 2012
  2. doi request reprint The ultrasound frequency determines the degree of intrinsic coagulation activation
    Thomas W Stief
    Institute of Laboratory Medicine and Pathobiochemistry, University Hospital Giessen and Marburg, Marburg, Germany
    Blood Coagul Fibrinolysis 23:440-4. 2012
  3. doi request reprint Contact activation of coagulation depends on the maximal lipophilic trigger concentration
    Thomas W Stief
    Institute of Laboratory Medicine and Pathobiochemistry, University Hospital Marburg, Germany
    Blood Coagul Fibrinolysis 23:296-8. 2012
  4. ncbi request reprint Coagulation activation by lipopolysaccharides
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 15:209-19. 2009
  5. ncbi request reprint Inhibition of thrombin in plasma by heparin or arginine
    Thomas W Stief
    Department of Clinical Chemistry Central Laboratory, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 13:146-53. 2007
  6. ncbi request reprint The efficiency of anti-activated factor X and anti-activated factor II anticoagulants
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Giessen and Marburg, Marburg, Germany
    Blood Coagul Fibrinolysis 18:265-9. 2007
  7. ncbi request reprint The anticoagulant capacity of plasmatic unfractionated heparin decreases at 23 degrees C
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Marburg, Germany
    Blood Coagul Fibrinolysis 18:209-12. 2007
  8. ncbi request reprint Analysis of hemostasis alterations in sepsis
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Giessen and Marburg, Marburg, Germany
    Blood Coagul Fibrinolysis 18:179-86. 2007
  9. ncbi request reprint Influence of coagulation factors on extrinsic thrombin generation
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Marburg, Germany
    Blood Coagul Fibrinolysis 18:105-12. 2007
  10. ncbi request reprint Influence of coagulation factors on intrinsic thrombin generation
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Marburg, Germany
    Blood Coagul Fibrinolysis 18:67-71. 2007

Detail Information

Publications49

  1. ncbi request reprint Determination of the anti-F10a or anti-F2a generation action of rivaroxaban or dabigatran
    Thomas W Stief
    Institute of Laboratory Medicine and Pathobiochemistry, University Hospital, Marburg, Germany
    Blood Coagul Fibrinolysis 23:619-21. 2012
    ..It is suggested to perform EXCA tests for each individual patient to determine the real drug dosage he needs to reach 10-20% of normal EXCA for therapy or 20-40% of normal EXCA for prophylaxis...
  2. doi request reprint The ultrasound frequency determines the degree of intrinsic coagulation activation
    Thomas W Stief
    Institute of Laboratory Medicine and Pathobiochemistry, University Hospital Giessen and Marburg, Marburg, Germany
    Blood Coagul Fibrinolysis 23:440-4. 2012
    ..Ultrasound frequencies of about 7 MHz might be used for therapeutic induction of coagulation activation, such as in patients with severe cerebral or hepatic hemorrhages...
  3. doi request reprint Contact activation of coagulation depends on the maximal lipophilic trigger concentration
    Thomas W Stief
    Institute of Laboratory Medicine and Pathobiochemistry, University Hospital Marburg, Germany
    Blood Coagul Fibrinolysis 23:296-8. 2012
    ..To study the action of drugs on the intrinsic system of plasma, plasma should be supplemented with the respective drug at maximal concentrations that are in the range of the maximal blood concentrations obtained in clinical medicine...
  4. ncbi request reprint Coagulation activation by lipopolysaccharides
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 15:209-19. 2009
    ..The complex interaction between cell fragments and hemostasis causes pathologic disseminated intravascular coagulation in sepsis...
  5. ncbi request reprint Inhibition of thrombin in plasma by heparin or arginine
    Thomas W Stief
    Department of Clinical Chemistry Central Laboratory, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 13:146-53. 2007
    ..Such pathologically elevated thrombin activity in blood or blood products must be prevented or inhibited. This study demonstrates the efficiency of two physiologic thrombin inhibitors: heparin and arginine...
  6. ncbi request reprint The efficiency of anti-activated factor X and anti-activated factor II anticoagulants
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Giessen and Marburg, Marburg, Germany
    Blood Coagul Fibrinolysis 18:265-9. 2007
    ..e. if about 0.1 IU/ml thrombin are inactivated, then thrombin cannot self-amplify its generation 10-fold). The efficiency of any clinically used plasmatic anticoagulant can be monitored in the EXCA...
  7. ncbi request reprint The anticoagulant capacity of plasmatic unfractionated heparin decreases at 23 degrees C
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Marburg, Germany
    Blood Coagul Fibrinolysis 18:209-12. 2007
    ..In contrast, the anticoagulant capacity of LMWH does not change significantly with temperature...
  8. ncbi request reprint Analysis of hemostasis alterations in sepsis
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Giessen and Marburg, Marburg, Germany
    Blood Coagul Fibrinolysis 18:179-86. 2007
    ..Whether the observed alterations are of clinical significance has to be determined in well defined patient groups...
  9. ncbi request reprint Influence of coagulation factors on extrinsic thrombin generation
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Marburg, Germany
    Blood Coagul Fibrinolysis 18:105-12. 2007
    ..Fibrinogen seems to act as antithrombin I; thrombin might be entrapped in the nascent fibrin. The EXCA is suitable to diagnose the level of extrinsic factors in patient plasma...
  10. ncbi request reprint Influence of coagulation factors on intrinsic thrombin generation
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Marburg, Germany
    Blood Coagul Fibrinolysis 18:67-71. 2007
    ..The INCA allows one to diagnose defects in the intrinsic coagulation system and might be a useful test to support development and characterization of new drugs targeted at the intrinsic generation of thrombin...
  11. ncbi request reprint Thrombin generation by hemolysis
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Giessen and Marburg, Germany
    Blood Coagul Fibrinolysis 18:61-6. 2007
    ..The recalcified coagulation activity assay allows one to quantify thrombin generation in critical hemolytic samples. It is suggested to find the appropriate pharmacologic dose of low-molecular-weight heparin...
  12. ncbi request reprint Arginine inhibits serpins
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Giessen Marburg, Germany
    Clin Appl Thromb Hemost 13:213-8. 2007
    ..In addition, these high concentrations of arginine might be a new gentle principle to inhibit pathogens that need serpins for their pathophysiology...
  13. ncbi request reprint Determination of the global fibrinolytic state
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Marburg, Germany
    Blood Coagul Fibrinolysis 18:479-87. 2007
    ..Chloramine (1.5 mmol/l) oxidation of the microclots favours their fibrinolytic breakdown, especially if lysis-resistant microclots are oxidized. The FIPA and CLA-PMN are new economical tests for the fibrinolytic state in patient blood...
  14. ncbi request reprint Singlet oxygen enhances intrinsic thrombolysis: the intrinsic oxidative clot lysis assay (INOXCLA)
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Giesson and Morburg, Germany
    Clin Appl Thromb Hemost 13:369-83. 2007
    ..Chloramines are important physiologic generators of nonradical excited singlet oxygen and enhance u-PA-mediated lysis of plasma clots. Based on the u-PA/chloramines coaction, a new global fibrinolysis assay has been derived...
  15. ncbi request reprint Inhibition of thrombin generation in recalcified plasma
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Giessen, Germany
    Blood Coagul Fibrinolysis 18:751-60. 2007
    ..It is suggested to analyze the individual patient's sensibility to certain plasmatic anticoagulants...
  16. ncbi request reprint The recalcified coagulation activity
    Thomas W Stief
    Department of Laboratory Medicine, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 14:447-53. 2008
    ..5- or 2-fold, respectively. Only thrombin activities in the ascending part of the thrombin generation curve (RECA-t(2)/RECAt( 1)>1) are valid. So a prothrombotic state in blood or plasma can be diagnosed...
  17. ncbi request reprint Kallikrein activates prothrombin
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Marburg, Germany
    Clin Appl Thromb Hemost 14:97-8. 2008
    ..Kallikrein can directly activate prothrombin; there is a shortcut in the intrinsic hemostasis system that generates catalytic amounts of thrombin without following the known intrinsic clotting pathway...
  18. ncbi request reprint Monitoring of functional plasminogen in the blood of patients on fibrinolytics
    Thomas W Stief
    Department of Laboratory Medicine, Hospital of Philipps University, Marburg, Germany
    Clin Appl Thromb Hemost 15:297-308. 2009
    ..The present findings support the profibrinolytic action of chloramines...
  19. doi request reprint Influence of the surface on thrombin generation
    T W Stief
    Department of Clinical Chemistry, University Hospital Giessen Marburg, Germany
    Int J Lab Hematol 30:269-77. 2008
    ..The reaction wall, the preincubation temperature, and hemolysis influences thrombin generation. The RECA allows to diagnose the prothrombotic capacity of any material...
  20. doi request reprint Intrinsic hemostasis activation by freezing and thawing of plasma
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Giessen and Marburg, Germany
    Clin Appl Thromb Hemost 15:59-64. 2009
    ..The intrinsic coagulation activity assay allows the measurement of low-grade contact activation of frozen/thawed plasma...
  21. ncbi request reprint The fibrinogen antigenic turbidimetric assay (FIATA): the X2x test--the corrected chi-square comparison against the control-mean
    Thomas W Stief
    Department of Clinical Chemistry, Central Laboratory, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 13:73-100. 2007
    ..Thus, the presented FIATA might contribute to extra hospital testing of patients for assessing their risk for myocardial or cerebral ischemia/infarction...
  22. ncbi request reprint Inhibition of extrinsic hemostasis activation by low-molecular-weight heparin
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital Giessen and Marburg, Germany
    Blood Coagul Fibrinolysis 17:633-7. 2006
    ..It is suggested to adjust the dosage of LMWH according to the EXCA value; about 30% of normal extrinsic thrombin generation might be the correct dose for prophylactic anticoagulation...
  23. ncbi request reprint The fibrinogen functional turbidimetric assay
    Thomas W Stief
    Department of Clinical Chemistry, Central Laboratory, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 14:84-96. 2008
    ..This assay has a diagnostic value in pathology-disseminated intravascular coagulation and in assessing risk for atherothrombosis...
  24. ncbi request reprint Regulation of hemostasis by singlet-oxygen (1DeltaO2*)
    Thomas W Stief
    Department of Clinical Chemistry, Philipps University Hospital, D 35033 Marburg, Germany
    Curr Vasc Pharmacol 2:357-62. 2004
    ..The biogenesis and reaction pattern of (1)DeltaO(2)(*) is of importance to understand the PMN-physiology in hemostasis, giving rise to new therapy forms of thromboatherothrombosis in man...
  25. ncbi request reprint Point of care: diagnostics in hemostasis--the wrong direction?
    Thomas W Stief
    Department of Clinical Chemistry, Philipps University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 9:191-5. 2003
    ..The rapid diagnosis of the acute hemostasis state of a patient should be performed in the 24-hour central laboratory that is connected to all hospital wards via a good pneumatic delivery...
  26. ncbi request reprint In vitro simulation of therapeutic plasmatic fibrinolysis
    T W Stief
    Department of Clinical Chemistry, Hospital of Philipps University Marburg, Germany
    Clin Appl Thromb Hemost 9:211-20. 2003
    ..Clinical thrombolysis might be improved by usage of more physiologic activators of thrombolysis, such as activators of polymorphonuclear neutrophils...
  27. ncbi request reprint Functional determination of plasmin in arginine-stabilized plasma
    T W Stief
    Department of Clinical Chemistry, Hospital of Philipps University Marburg, Germany
    Clin Appl Thromb Hemost 11:303-9. 2005
    ....
  28. ncbi request reprint Functional determination of plasminogen activator in arginine-stabilized plasma
    T W Stief
    Department of Clinical Chemistry, Hospital of Philipps University, Marburg, Germany
    Clin Appl Thromb Hemost 11:311-23. 2005
    ....
  29. ncbi request reprint Specific determination of plasmatic thrombin activity
    T W Stief
    Department of Clinical Chemistry, University Hospital, Giessen and Marburg, Germany
    Clin Appl Thromb Hemost 12:324-9. 2006
    ..This technique allows to standardize the thrombin activity generated in any biologic system in international thrombin units...
  30. ncbi request reprint The intrinsic coagulation activity assay
    Thomas W Stief
    Department of Clinical Chemistry Central Laboratory, University Hospital Giessen and Marburg, Germany
    Blood Coagul Fibrinolysis 17:369-78. 2006
    ....
  31. ncbi request reprint Monitoring of plasmin and plasminogen activator activity in blood of patients under fibrinolytic treatment by reteplase
    T W Stief
    Department of Laboratory Medicine, Hospital of Philipps University Marburg, Germany
    Clin Appl Thromb Hemost 12:213-8. 2006
    ..e., polymorphonuclear phagocytes (PMN) that can be activated by singlet oxygen ((1)O(2)). PMN might be superior to PA in selective lysis of pathologic thrombi...
  32. ncbi request reprint Thrombin generation by exposure of blood to endotoxin: a simple model to study disseminated intravascular coagulation
    T W Stief
    Department of Clinical Chemistry, University Hospital Giessen Marburg, Germany
    Clin Appl Thromb Hemost 12:137-61. 2006
    ..Arginine at plasma concentrations of 10 to 50 mM might be a new therapeutic for sepsis-PDIC...
  33. ncbi request reprint In vitro simulation of therapeutic thrombolysis with microtiter plate clot-lysis assay
    Thomas W Stief
    Department of Clinical Chemistry, Hospital of Philipps University, Marburg, Germany
    Clin Appl Thromb Hemost 12:21-32. 2006
    ..It is suggested that different thrombolytic agents or concentrations thereof would have a different clinical outcome in different individuals...
  34. ncbi request reprint Singlet oxygen inactivates fibrinogen, factor V, factor VIII, factor X, and platelet aggregation of human blood
    T W Stief
    Institute of Clinical Chemistry, Philipps University, D 35033, Marburg, Germany
    Thromb Res 97:473-80. 2000
    ..Via 1O(2) generation, polymorphonuclear leukocytes can generate a local pericellular zone of anticoagulation. The data suggest that the cell signal 1O(2) in physiological amounts is an antithrombotic agent...
  35. ncbi request reprint Singlet oxygen inhibits agonist-induced P-selectin expression and formation of platelet aggregates
    T W Stief
    Department of Clinical Chemistry and Molecular Diagnostics, Hospital of Philipps University, Marburg, Germany
    Clin Appl Thromb Hemost 7:219-24. 2001
    ..The physiologic signal action and the direct anticoagulant action of 1O2 (released by chloramines such as vancomycin) might be a new principle for pharmacologic intervention in atherothrombosis...
  36. ncbi request reprint Thrombin converts singlet oxygen (1O2)-oxidized fibrinogen into a soluble t-PA cofactor. A new method for preparing a stimulator for functional t-PA assays
    T W Stief
    Institute of Clinical Chemistry and Molecular Diagnostics, Philipps University, Marburg, Germany
    Ann Hematol 80:189-94. 2001
    ..Thus, since oxidized fibrin monomer is a t-PA cofactor, thrombin-degraded oxidized fibrinogen can be used as a stimulator in functional t-PA assays...
  37. doi request reprint The extrinsic coagulation activity assay
    Thomas W Stief
    Department of Clinical Chemistry, Central Laboratory, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 14:303-18. 2008
    ....
  38. ncbi request reprint Singlet oxygen ((1)O2) inactivates plasmatic free and complexed alpha2-macroglobulin
    T W Stief
    Institute of Clinical Chemistry and Molecular Diagnostics, Philipps University, Marburg, Germany
    Thromb Res 98:541-7. 2000
    ..In local areas of inflammation or thrombolysis, activated phagocytes could create microenvironments of uncontrolled protease activity by generation of (1)O2...
  39. ncbi request reprint Arginine inhibits hemostasis activation
    T W Stief
    Department of Clinical Chemistry and Molecular Diagnostics, Hospital of Philipps University, D 35033 Marburg, Germany
    Thromb Res 104:265-74. 2001
    ..Therefore, there is need for a simple physiologic inhibitor of hemostasis activation both in diagnosis and therapy of hemostasis activation...
  40. ncbi request reprint A simple screening assay for certain fibrinolysis parameters (FIPA)
    T W Stief
    Institute of Clinical Chemistry, University Hospital of Philipps University, Marburg, Germany
    Thromb Res 97:231-7. 2000
    ..684) with the plasminogen activator inhibitor-1 activity of patient samples. The plasmatic fibrinolysis parameters assay is a simple, rapid, and economic functional test for several clinical relevant fibrinolysis parameters...
  41. ncbi request reprint Singlet oxygen potentiates thrombolysis
    Thomas W Stief
    Department of Clinical Chemistry, University of Giessen and Marburg, Germany
    Clin Appl Thromb Hemost 13:259-78. 2007
    ..The present global assay technique imitates the u-PA/(1)O(2) aspects of physiologic thrombolysis by PMN...
  42. ncbi request reprint Singlet oxygen (1O(2)) disrupts platelet aggregates
    T W Stief
    Department of Clinical Chemistry and Molecular Diagnostics, Philipps University, D 35033, Marburg, Germany
    Thromb Res 104:361-70. 2001
    ..Since 1O(2) inhibits platelet aggregation, we became interested in a possible oxidant mediated reversibility of platelet aggregation...
  43. ncbi request reprint Singlet oxygen (1O2)-oxidazable lipids in the HIV membrane, new targets for AIDS therapy?
    Thomas W Stief
    Institute of Clinical Chemistry, University Hospital, Marburg, Germany
    Med Hypotheses 60:575-7. 2003
    ..Therefore, it is proposed to consider generators of 1O(2) as a new form of AIDS therapy...
  44. ncbi request reprint The physiology and pharmacology of singlet oxygen
    Thomas W Stief
    Department of Clinical Chemistry and Molecular Diagnostics, University Hospital, Marburg, Germany
    Med Hypotheses 60:567-72. 2003
    ....
  45. ncbi request reprint In vitro simulation of extremely activated thrombolysis
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 14:238-40. 2008
    ..The risk for severe intracranial hemorrhage in massive thrombolysis might be much lower than the lethality of a massive pulmonary embolism. Extremely activated plasmatic thrombolysis could be clinically indicated...
  46. ncbi request reprint In vitro simulation of thrombolysis inhibition
    Thomas W Stief
    Department of Clinical Chemistry, University Hospital, Marburg, Germany
    Clin Appl Thromb Hemost 14:234-7. 2008
    ..Lysine might be a new antifibrinolytic drug with a clinically interesting rapid pharmacokinetic. This data help correct dosing of antifibrinolytics to patients with hyperfibrinolysis...
  47. ncbi request reprint Specific determination of plasmatic plasmin activity
    Thomas W Stief
    Blood Coagul Fibrinolysis 18:213. 2007
  48. ncbi request reprint NADPH-oxidase might also act as an antithrombotic
    Thomas W Stief
    Circulation 107:e8; author reply e8. 2003
  49. ncbi request reprint Thrombin and plasmin activity in semen
    Thomas W Stief
    Blood Coagul Fibrinolysis 18:386-7. 2007