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Genomes and Genes | Roland LillSummaryAffiliation: Philipps University Country: Germany Publications
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The Hsp70 chaperone Ssq1p is dispensable for iron-sulfur cluster formation on the scaffold protein Isu1pRafal Dutkiewicz
Department of Molecular and Cellular Biology, Faculty of Biotechnology, University of Gdansk, 24 Kladki, 80 822 Gdansk, Poland
J Biol Chem 281:7801-8. 2006..Hence, consistent with previous in vivo data, these chaperones likely function in steps subsequent to the de novo synthesis of the Fe/S cluster on Isu1p...
The role of mitochondria in cellular iron-sulfur protein biogenesis and iron metabolismRoland Lill
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 6, 35033 Marburg, Germany
Biochim Biophys Acta 1823:1491-508. 2012..Thus, Fe/S protein biogenesis and cellular iron metabolism are tightly linked to coordinate iron supply and utilization. This article is part of a Special Issue entitled: Cell Biology of Metals...- Iron-sulfur protein biogenesis in eukaryotes: components and mechanismsRoland Lill
Institut fur Zytobiologie, Philipps Universitat Marburg, 35037 Marburg, Germany
Annu Rev Cell Dev Biol 22:457-86. 2006..This process requires the participation of mitochondria that export a still unknown component via the ISC export machinery, including an ABC transporter... - Mechanisms of iron-sulfur protein maturation in mitochondria, cytosol and nucleus of eukaryotesRoland Lill
Institut fur Zytobiologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35037 Marburg, Germany
Biochim Biophys Acta 1763:652-67. 2006.... - Maturation of iron-sulfur proteins in eukaryotes: mechanisms, connected processes, and diseasesRoland Lill
Institut fur Zytobiologie, Philipps Universitat Marburg, Marburg, Germany
Annu Rev Biochem 77:669-700. 2008..Numerous diseases including several neurodegenerative and hematological disorders have been associated with defects in Fe/S protein biogenesis, underlining the central importance of this process for life... - Is there an answer? Why are mitochondria essential for life?Roland Lill
Institut für Zytobiologie und Zytopathologie der Philipps Universität Marburg, Robert Koch Str 6, 35033 Marburg, Germany
IUBMB Life 57:701-3. 2005 - Function and biogenesis of iron-sulphur proteinsRoland Lill
Institut fur Zytobiologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
Nature 460:831-8. 2009..The importance of Fe-S proteins for life is documented by an increasing number of diseases linked to these components and their biogenesis... - Functional characterization of the eukaryotic cysteine desulfurase Nfs1p from Saccharomyces cerevisiaeUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35033 Marburg, Germany
J Biol Chem 279:36906-15. 2004..The implication of these findings for the structural stability of Nfs1p and for its targeting mechanism to mitochondria and cytosol/nucleus will be discussed... 
The essential WD40 protein Cia1 is involved in a late step of cytosolic and nuclear iron-sulfur protein assemblyJanneke Balk
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Germany
Mol Cell Biol 25:10833-41. 2005..Taken together, our results indicate that Cia1 is a new member of the cytosolic Fe/S protein assembly (CIA) machinery participating in a step after Nbp35 and Nar1...- Humans possess two mitochondrial ferredoxins, Fdx1 and Fdx2, with distinct roles in steroidogenesis, heme, and Fe/S cluster biosynthesisAlex D Sheftel
Institut fur Zytobiologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35033 Marburg, Germany
Proc Natl Acad Sci U S A 107:11775-80. 2010..We conclude that mammals depend on two distinct mitochondrial ferredoxins for the specific production of either steroid hormones or heme A and Fe/S proteins... - Cellular and mitochondrial remodeling upon defects in iron-sulfur protein biogenesisAnja Hausmann
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, Marburg, Germany
J Biol Chem 283:8318-30. 2008..Together, our data suggest a model in which mitochondria perform a global regulatory role in numerous cellular processes linked to iron homeostasis... - Human ind1, an iron-sulfur cluster assembly factor for respiratory complex IAlex D Sheftel
Institut fur Zytobiologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35033 Marburg, Germany
Mol Cell Biol 29:6059-73. 2009..Together, these data identify huInd1 as a new assembly factor for human respiratory complex I with a possible role in the delivery of one or more Fe/S clusters to complex I subunits... - Iron regulation through the back door: iron-dependent metabolite levels contribute to transcriptional adaptation to iron deprivation in Saccharomyces cerevisiaeJessica Ihrig
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35032 Marburg, Germany
Eukaryot Cell 9:460-71. 2010..Only the combination of the two processes provides a quantitative description of the response to iron deprivation in yeast... - MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrityOliver Stehling
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 6, 35033 Marburg, Germany
Science 337:195-9. 2012..The function of MMS19 in the maturation of crucial components of DNA metabolism may explain the sensitivity of MMS19 mutants to DNA damage and the presence of extended telomeres... - Components involved in assembly and dislocation of iron-sulfur clusters on the scaffold protein Isu1pUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
EMBO J 22:4815-25. 2003..We propose a model that dissects Fe/S cluster biogenesis into two major steps and assigns its central components to one of these two steps... - A bridging [4Fe-4S] cluster and nucleotide binding are essential for function of the Cfd1-Nbp35 complex as a scaffold in iron-sulfur protein maturationDaili J A Netz
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35032 Marburg, Germany
J Biol Chem 287:12365-78. 2012.... - Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulationAnnette Biederbick
Institut fur Zytobiologie, Philipps Universitat Marburg, Robert Koch Str 6, 35037 Marburg, Germany
Mol Cell Biol 26:5675-87. 2006..The results have implications for the regulation of iron homeostasis by cytosolic iron regulatory protein 1... - The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosolDaili J A Netz
Institut fur Zytobiologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
Nat Chem Biol 3:278-86. 2007..Our data suggest that the Cfd1-Nbp35 complex functions as a novel scaffold for [Fe-S] cluster assembly in the eukaryotic cytosol... - The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturationAlex D Sheftel
Institut fur Zytobiologie, Philipps Universitat Marburg, Marburg, Germany
Mol Biol Cell 23:1157-66. 2012..Taken together, our data suggest that ISCA1, ISCA2, and IBA57 are specifically involved in the maturation of mitochondrial [4Fe-4S] proteins functioning late in the ISC assembly pathway... - Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasisOliver Stehling
Institut für Zytobiologie und Zytopathologie, Philipps Universitat, Robert Koch Str 6, 35033 Marburg, Germany
Mol Cell Biol 28:5517-28. 2008.... - Crucial role of conserved cysteine residues in the assembly of two iron-sulfur clusters on the CIA protein Nar1Eugen Urzica
Institut fur Zytobiologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
Biochemistry 48:4946-58. 2009..The vicinity of the two Fe/S centers suggests a close functional cooperation during cytosolic Fe/S protein maturation... - Mitochondrial Iba57p is required for Fe/S cluster formation on aconitase and activation of radical SAM enzymesCristy Gelling
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 6, 35032 Marburg, Germany
Mol Cell Biol 28:1851-61. 2008..In keeping with this idea, the human IBA57 homolog C1orf69 complements the iba57Delta growth defects, demonstrating its conserved function throughout the eukaryotic kingdom... - A specific role of the yeast mitochondrial carriers MRS3/4p in mitochondrial iron acquisition under iron-limiting conditionsUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 6, 35033 Marburg, Germany
J Biol Chem 278:40612-20. 2003..Mrs3/4p function in mitochondrial iron transport becomes evident under iron-limiting conditions only, indicating that the two carriers do not represent the sole system for mitochondrial iron acquisition... - Tah18 transfers electrons to Dre2 in cytosolic iron-sulfur protein biogenesisDaili J A Netz
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Marburg, Germany
Nat Chem Biol 6:758-65. 2010..The pathway is conserved in eukaryotes, as human Ndor1-Ciapin1 proteins can functionally replace yeast Tah18-Dre2... - The yeast frataxin homolog Yfh1p plays a specific role in the maturation of cellular Fe/S proteinsUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 5, 35033 Marburg, Germany
Hum Mol Genet 11:2025-36. 2002..Friedreich's ataxia may therefore represent a disorder caused by defects in Fe/S protein maturation... - Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteinsUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35032 Marburg, Germany
J Biol Chem 286:41205-16. 2011..Taken together, these findings define Isa1, Isa2, and Iba57 as a specialized, late-acting ISC assembly subsystem that is specifically dedicated to the maturation of mitochondrial [4Fe-4S] proteins... - Investigation of iron-sulfur protein maturation in eukaryotesOliver Stehling
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Germany
Methods Mol Biol 372:325-42. 2007..These approaches are crucial to elucidate the mechanisms underlying the maturation of Fe-S proteins and may aid in the identification of new members of this evolutionary ancient process... - The eukaryotic P loop NTPase Nbp35: an essential component of the cytosolic and nuclear iron-sulfur protein assembly machineryAnja Hausmann
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
Proc Natl Acad Sci U S A 102:3266-71. 2005..The findings provide strong evidence for the existence of a highly conserved and essential machinery dedicated to assembling cytosolic and nuclear Fe/S proteins... - The yeast scaffold proteins Isu1p and Isu2p are required inside mitochondria for maturation of cytosolic Fe/S proteinsJana Gerber
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
Mol Cell Biol 24:4848-57. 2004..Together, our data suggest that the Isu proteins need to be localized in mitochondria to fulfill their functional requirement in Fe/S protein maturation in the cytosol... - Stimulation of the ATPase activity of the yeast mitochondrial ABC transporter Atm1p by thiol compoundsGrit Kuhnke
Institut für Zytobiologie und Zytopathologie der Philipps Universität Marburg, Marburg, Germany
Mol Membr Biol 23:173-84. 2006..We speculate that the physiological substrate of Atm1p may contain multiple sulfhydryl groups in a peptidic environment... - Iron-sulfur proteins in health and diseaseAlex Sheftel
Institut fur Zytobiologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
Trends Endocrinol Metab 21:302-14. 2010.... - Analysis of iron-sulfur protein maturation in eukaryotesAntonio J Pierik
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Marburg, Germany
Nat Protoc 4:753-66. 2009..Comprehensive analysis of one biogenesis component or target Fe/S protein takes about 10 d... - Methods for studying iron metabolism in yeast mitochondriaSabine Molik
, , 35033 Marburg, Germany
Methods Cell Biol 80:261-80. 2007 - Cytosolic monothiol glutaredoxins function in intracellular iron sensing and trafficking via their bound iron-sulfur clusterUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, 35032 Marburg, Germany
Cell Metab 12:373-85. 2010..Collectively, our study uncovers an important role of monothiol glutaredoxins in cellular iron metabolism, with a surprising connection to cellular redox and sulfur metabolisms... - The multidomain thioredoxin-monothiol glutaredoxins represent a distinct functional groupBastian Hoffmann
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Germany
Antioxid Redox Signal 15:19-30. 2011..This interaction is essential for the regulation of Aft1. Collectively, our analysis demonstrates that the multidomain monothiol Grxs form a unique protein family distinct from that of the single domain Grxs... - Eukaryotic DNA polymerases require an iron-sulfur cluster for the formation of active complexesDaili J A Netz
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Marburg, Germany
Nat Chem Biol 8:125-32. 2012..Together, our findings show that the function of eukaryotic replicative DNA polymerases crucially depends on different metallocenters for accessory subunit recruitment and replisome stability... - The hydrogenase-like Nar1p is essential for maturation of cytosolic and nuclear iron-sulphur proteinsJanneke Balk
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse, Marburg, Germany
EMBO J 23:2105-15. 2004..In conclusion, Nar1p represents a crucial, novel component of the emerging cytosolic Fe/S protein assembly machinery that catalyses an essential and ancient process in eukaryotes... - The ISC [corrected] proteins Isa1 and Isa2 are required for the function but not for the de novo synthesis of the Fe/S clusters of biotin synthase in Saccharomyces cerevisiaeUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35033 Marburg, Germany
Eukaryot Cell 6:495-504. 2007..Thus, the Isa proteins are crucial for the in vivo function of biotin synthase but not for the de novo synthesis of its Fe/S clusters. Our data demonstrate that the Isa proteins are essential for the catalytic activity of Bio2 in vivo... - An interaction between frataxin and Isu1/Nfs1 that is crucial for Fe/S cluster synthesis on Isu1Jana Gerber
Institute for Cytobiology and Cytopathology, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
EMBO Rep 4:906-11. 2003..The iron-dependent binding of Yfh1 to Isu1/Nfs1 suggests a role of frataxin/Yfh1 in iron loading of the Isu scaffold proteins... - The heme synthesis defect of mutants impaired in mitochondrial iron-sulfur protein biogenesis is caused by reversible inhibition of ferrochelataseHeike Lange
, Robert-Koch-Strasse 6, 35033 Marburg, Germany
J Biol Chem 279:29101-8. 2004..This inhibitory mechanism may be used for regulation of iron distribution between the two iron-consuming processes... - Iron-sulfur protein maturation in human cells: evidence for a function of frataxinOliver Stehling
Institut für Zytobiologie und Zytopathologie, Philipps Universitat, Marburg, Germany
Hum Mol Genet 13:3007-15. 2004..These results demonstrate (i) that frataxin is a component of the human Fe/S cluster assembly machinery and (ii) that it plays a role in the maturation of both mitochondrial and cytosolic Fe/S proteins... - Iron-sulfur-protein biogenesis in eukaryotesRoland Lill
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
Trends Biochem Sci 30:133-41. 2005..The essential character of Fe-S-protein biogenesis in eukaryotes and its importance for human disease identifies this evolutionary ancient process as one of the most important biosynthetic pathways of life... - The power plant of the cell is also a smithy: the emerging role of mitochondria in cellular iron homeostasisAlex D Sheftel
Institut fur Zytobiologie, Philipps Universitat Marburg, Germany
Ann Med 41:82-99. 2009..The goal of this article is to provide an overview of how mitochondria acquire and use iron and examine the ramifications of disturbances in these processes on overall cellular iron homeostasis... - Chapter 12 Controlled expression of iron-sulfur cluster assembly components for respiratory chain complexes in mammalian cellsOliver Stehling
Institut für Zytobiologie and Zytopathologie, Philipps Universitat, Marburg, Germany
Methods Enzymol 456:209-31. 2009..Disorders in Fe/S cluster assembly are candidate causes for defects in respiratory complex assembly of unknown etiology... - The cell's cookbook for iron--sulfur clusters: recipes for fool's gold?Janneke Balk
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35033 Marburg, Germany
Chembiochem 5:1044-9. 2004 - Characterization of iron-sulfur protein assembly in isolated mitochondria. A requirement for ATP, NADH, and reduced ironUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie der Philipps Universität Marburg, Robert Koch Strasse 5, 35033 Marburg, Germany
J Biol Chem 277:29810-6. 2002..Our results represent the first in vitro reconstitution of the entire pathway of Fe/S protein maturation... - Lights on iron-sulfur clustersChristopher Horst Lillig
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, 35032 Marburg, Germany
Chem Biol 16:1213-4. 2009..In this issue of Chemistry and Biology, Hoff et al. introduce a non-invasive fluorescence-based method to trace the formation of iron-sulfur clusters at high sensitivity... - Application of the DNA-specific dye EvaGreen for the routine quantification of DNA in microplatesJessica Ihrig
, , 35033 Marburg, Germany
Anal Biochem 359:265-7. 2006 - Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteinsNils Wiedemann
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Freiburg, Germany
EMBO J 25:184-95. 2006..We conclude that Isd11 is an indispensable eukaryotic component of the mitochondrial machinery for biogenesis of Fe/S proteins... - Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesisJulian C Rutherford
University of Utah Health Sciences Center, Department of Medicine, Salt Lake City, Utah 84132, USA
J Biol Chem 280:10135-40. 2005..Although Aft1 and Aft2 sense a signal emanating from the Fe-S cluster biogenesis pathway, there is no indication that the proteins are inhibited by direct binding of an Fe-S cluster... - The effects of mitochondrial iron homeostasis on cofactor specificity of superoxide dismutase 2Mei Yang
Division of Toxicological Sciences, Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
EMBO J 25:1775-83. 2006..The ability to control this reactive pool of iron is critical to maintaining SOD2 activity and has important potential implications for oxidative stress in disorders of iron overload... - Localization and functionality of microsporidian iron-sulphur cluster assembly proteinsAlina V Goldberg
Institute for Cell and Molecular Biosciences, The Catherine Cookson Building, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
Nature 452:624-8. 2008..Together, our studies identify the essential biosynthetic process of Fe-S protein assembly as a key function of microsporidian mitosomes... - Thio modification of yeast cytosolic tRNA is an iron-sulfur protein-dependent pathwayYumi Nakai
Department of Biochemistry, Osaka Medical College, 2 7 Daigakumachi, Takatsuki 569 8686, Japan
Mol Cell Biol 27:2841-7. 2007.... - The iron-sulphur protein Ind1 is required for effective complex I assemblyKatrine Bych
Department of Plant Sciences, University of Cambridge, Cambridge, UK
EMBO J 27:1736-46. 2008..Our data suggest that Ind1 facilitates the assembly of Fe-S cofactors and subunits of complex I... - Bacterial ApbC can bind and effectively transfer iron-sulfur clustersJeffrey M Boyd
Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
Biochemistry 47:8195-202. 2008.... - EPR and Mössbauer spectroscopy of intact mitochondria isolated from Yah1p-depleted Saccharomyces cerevisiaeRen Miao
Department of Chemistry, Texas A and M University, College Station, Texas 77843 3255, USA
Biochemistry 47:9888-99. 2008..The efficacy of reducing accumulated Fe along with chelating it should be considered as a strategy for its removal in diseases involving such accumulations... - Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondriaGyula Kispal
Institute of Biochemistry, University Medical School of Pecs, Hungary
EMBO J 24:589-98. 2005..Thus, ribosome biogenesis and function are intimately linked to the crucial role of mitochondria in the maturation of the essential Fe/S protein Rli1p... - Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesisVasundara Srinivasan
Department of Molecular Membrane Biology, Max Planck Institute for Biophysics, Max von Laue Str 3, D 60438 Frankfurt M, Germany
Structure 15:1246-57. 2007..We show that Ciao1 can functionally replace Cia1 and support cytosolic Fe/S protein biogenesis. Hence, our structural and biochemical studies indicate the conservation of Cia1 function in eukaryotes... - Role of glutaredoxin-3 and glutaredoxin-4 in the iron regulation of the Aft1 transcriptional activator in Saccharomyces cerevisiaeLuis Ojeda
Departments of Medicine and Biochemistry, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
J Biol Chem 281:17661-9. 2006..Thus, Grx3 and Grx4 are novel components required for Aft1 iron regulation that most likely occurs in the nucleus... - Maturation of cytosolic iron-sulfur proteins requires glutathioneKatalin Sipos
Institute of Biochemistry, Medical Faculty, University of Pecs, Szigeti ut 12, 7624 Pecs, Hungary
J Biol Chem 277:26944-9. 2002..Together, our data demonstrate that glutathione, in addition to its protective role against oxidative damage, performs a novel and specific function in the maturation of cytosolic Fe/S proteins... - Iron-dependent degradation of apo-IRP1 by the ubiquitin-proteasome pathwayJian Wang
Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, 3755 Cote Ste Catherine Road, Montreal, Quebec H3T 1E2, Canada
Mol Cell Biol 27:2423-30. 2007..Collectively, these data uncover a mechanism for the regulation of IRP1 abundance as a means to control its RNA-binding activity, when the ISC assembly pathway is impaired...