Dagmar Fischer

Summary

Affiliation: Philipps University
Country: Germany

Publications

  1. ncbi Poly(diallyldimethylammonium chlorides) and their N-methyl-N-vinylacetamide copolymer-based DNA-polyplexes: role of molecular weight and charge density in complex formation, stability, and in vitro activity
    Dagmar Fischer
    Department of Pharmaceutics and Biopharmacy, Philipps University of Marburg, Ketzerbach 63, 35032 Marburg Lahn, Germany
    Int J Pharm 280:253-69. 2004
  2. ncbi In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis
    Dagmar Fischer
    Department of Pharmaceutics and Biopharmacy, University of Marburg, Ketzerbach 63, 35032, Marburg, Germany
    Biomaterials 24:1121-31. 2003
  3. ncbi Copolymers of ethylene imine and N-(2-hydroxyethyl)-ethylene imine as tools to study effects of polymer structure on physicochemical and biological properties of DNA complexes
    Dagmar Fischer
    Department of Pharmaceutics and Biopharmacy, University of Marburg, Ketzerbach 63, 35032 Marburg, Germany
    Bioconjug Chem 13:1124-33. 2002
  4. ncbi Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine
    Klaus Kunath
    Department of Pharmaceutics and Biopharmacy, Philipps University of Marburg, Ketzerbach 63, D-35032, Marburg/Lahn, Germany
    J Control Release 89:113-25. 2003
  5. ncbi Galactose-PEI-DNA complexes for targeted gene delivery: degree of substitution affects complex size and transfection efficiency
    Klaus Kunath
    Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Ketzerbach 63, 35032, Marburg, Germany
    J Control Release 88:159-72. 2003
  6. ncbi Polyethylenimine-graft-poly(ethylene glycol) copolymers: influence of copolymer block structure on DNA complexation and biological activities as gene delivery system
    Holger Petersen
    Department of Pharmaceutics and Biopharmacy, Philipps University, Ketzerbach 63, D-35032 Marburg, Germany
    Bioconjug Chem 13:845-54. 2002
  7. ncbi Uptake and transport of PEG-graft-trimethyl-chitosan copolymer-insulin nanocomplexes by epithelial cells
    Shirui Mao
    Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Ketzerbach 63, D-35032, Marburg, Germany
    Pharm Res 22:2058-68. 2005
  8. ncbi Poly(ethylenimine-co-L-lactamide-co-succinamide): a biodegradable polyethylenimine derivative with an advantageous pH-dependent hydrolytic degradation for gene delivery
    Holger Petersen
    Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Ketzerbach 63, D-35032 Marburg, Germany
    Bioconjug Chem 13:812-21. 2002
  9. ncbi The structure of PEG-modified poly(ethylene imines) influences biodistribution and pharmacokinetics of their complexes with NF-kappaB decoy in mice
    Klaus Kunath
    Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Germany
    Pharm Res 19:810-7. 2002
  10. ncbi Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives
    Michael Neu
    Department of Pharmaceutics and Biopharmacy, Philipps University, Ketzerbach 63, 35037 Marburg, Germany
    J Gene Med 7:992-1009. 2005

Collaborators

Detail Information

Publications14

  1. ncbi Poly(diallyldimethylammonium chlorides) and their N-methyl-N-vinylacetamide copolymer-based DNA-polyplexes: role of molecular weight and charge density in complex formation, stability, and in vitro activity
    Dagmar Fischer
    Department of Pharmaceutics and Biopharmacy, Philipps University of Marburg, Ketzerbach 63, 35032 Marburg Lahn, Germany
    Int J Pharm 280:253-69. 2004
    ..In conclusion, the DADMACs are an interesting tool to study structure-function-relationships due to the specific adjustment of molecular weight as well as number and density of charges...
  2. ncbi In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis
    Dagmar Fischer
    Department of Pharmaceutics and Biopharmacy, University of Marburg, Ketzerbach 63, 35032, Marburg, Germany
    Biomaterials 24:1121-31. 2003
    ..fmk did not inhibit poly(ethylenimine)-induced cell damage. Insights into the structure-toxicity relationship are necessary to optimize the cytotoxicity and biocompatibility of non-viral gene delivery systems...
  3. ncbi Copolymers of ethylene imine and N-(2-hydroxyethyl)-ethylene imine as tools to study effects of polymer structure on physicochemical and biological properties of DNA complexes
    Dagmar Fischer
    Department of Pharmaceutics and Biopharmacy, University of Marburg, Ketzerbach 63, 35032 Marburg, Germany
    Bioconjug Chem 13:1124-33. 2002
    ....
  4. ncbi Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine
    Klaus Kunath
    Department of Pharmaceutics and Biopharmacy, Philipps University of Marburg, Ketzerbach 63, D-35032, Marburg/Lahn, Germany
    J Control Release 89:113-25. 2003
    ....
  5. ncbi Galactose-PEI-DNA complexes for targeted gene delivery: degree of substitution affects complex size and transfection efficiency
    Klaus Kunath
    Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Ketzerbach 63, 35032, Marburg, Germany
    J Control Release 88:159-72. 2003
    ..In NIH-3T3 mouse fibroblasts, increasing the DS led to a decreased transfection efficiency for all N/P ratios. Our study highlights the necessity of careful optimization of polyplex composition for active gene targeting...
  6. ncbi Polyethylenimine-graft-poly(ethylene glycol) copolymers: influence of copolymer block structure on DNA complexation and biological activities as gene delivery system
    Holger Petersen
    Department of Pharmaceutics and Biopharmacy, Philipps University, Ketzerbach 63, D-35032 Marburg, Germany
    Bioconjug Chem 13:845-54. 2002
    ..These results provide a basis for the rational design of block copolymer gene delivery systems...
  7. ncbi Uptake and transport of PEG-graft-trimethyl-chitosan copolymer-insulin nanocomplexes by epithelial cells
    Shirui Mao
    Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Ketzerbach 63, D-35032, Marburg, Germany
    Pharm Res 22:2058-68. 2005
    ..This implies that PEGylated trimethyl chitosan complexes with insulin need further optimization and the Caco-2 cell line is a predictable in vitro cell culture model for drug absorption...
  8. ncbi Poly(ethylenimine-co-L-lactamide-co-succinamide): a biodegradable polyethylenimine derivative with an advantageous pH-dependent hydrolytic degradation for gene delivery
    Holger Petersen
    Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Ketzerbach 63, D-35032 Marburg, Germany
    Bioconjug Chem 13:812-21. 2002
    ..This makes P(EI-co-LSA) a promising candidate for long-term gene therapy where biocompatibility and biodegradability become increasingly important...
  9. ncbi The structure of PEG-modified poly(ethylene imines) influences biodistribution and pharmacokinetics of their complexes with NF-kappaB decoy in mice
    Klaus Kunath
    Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Germany
    Pharm Res 19:810-7. 2002
    ..CONCLUSIONS: A sufficiently high density of PEG molecules is necessary to effectively prevent opsonization and thereby rapid clearance from blood stream...
  10. ncbi Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives
    Michael Neu
    Department of Pharmaceutics and Biopharmacy, Philipps University, Ketzerbach 63, 35037 Marburg, Germany
    J Gene Med 7:992-1009. 2005
    ..Rational design of optimized polycations is an objective for further research and may provide the basis for a successful cationic polymer-based gene delivery system in the future...
  11. ncbi Effect of poly(ethylene imine) molecular weight and pegylation on organ distribution and pharmacokinetics of polyplexes with oligodeoxynucleotides in mice
    Dagmar Fischer
    Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, USA
    Drug Metab Dispos 32:983-92. 2004
    ....
  12. ncbi Inhibition of monocyte adhesion on brain-derived endothelial cells by NF-kappaB decoy/polyethylenimine complexes
    Dagmar Fischer
    Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, 1300 Coulter Dr, Amarillo, TX 79106, USA
    J Gene Med 7:1063-76. 2005
    ..We investigated the inhibition of monocyte adhesion by NF-kappaB transcription factor decoys complexed with polyethylenimines (PEIs) of different molecular weights and structures (800, 25, and 2.7 kDa PEI)...
  13. ncbi Intracellular processing of poly(ethylene imine)/ribozyme complexes can be observed in living cells by using confocal laser scanning microscopy and inhibitor experiments
    Thomas Merdan
    Department of Pharmaceutics and Biopharmacy, Philipps University, Marburg, Germany
    Pharm Res 19:140-6. 2002
    ..CLSM, in conjunction with living cell microscopy, is a promising tool for studying the subcellular fate of polyplexes in nucleic acid/gene delivery...
  14. ncbi Targeted delivery of complexes of biotin-PEG-polyethylenimine and NF-kappaB decoys to brain-derived endothelial cells in vitro
    Raktima Bhattacharya
    Department of Pharmaceutical Sciences, Texas Tech University Health Sciences Center School of Pharmacy, 1300 Coulter Drive, Amarillo, Texas 79106, USA
    Pharm Res 25:605-15. 2008
    ..To evaluate the effect of re-directing the uptake mechanism of polyplexes containing oligodeoxynucleotide (ODN) decoys to nuclear factor kappa B (NF-kappaB) from absorptive-mediated to receptor-mediated endocytosis...