Michael W Pfaffl

Summary

Country: Germany

Publications

  1. doi Changes in the miRNA profile under the influence of anabolic steroids in bovine liver
    Christiane Becker
    Physiology Weihenstephan, Technische Universitaet Muenchen, Weihenstephaner Berg 3, 85384 Freising, Germany
    Analyst 136:1204-9. 2011
  2. ncbi Development of clenbuterol reference materials: lyophilized bovine eye samples free of clenbuterol (CRM 673) and containing clenbuterol (CRM 674). Part 1. Preparation, homogeneity and stability
    M W Pfaffl
    Technische Universitat Munchen, Institut fur Physiologie, Freising Weihenstephan, Germany
    Fresenius J Anal Chem 371:1086-91. 2001
  3. ncbi Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations
    Michael W Pfaffl
    Physiology, FML Weihenstephan, Centre of Life and Food Science, Technical University of Munich, Germany
    Biotechnol Lett 26:509-15. 2004
  4. ncbi Influence of zinc deficiency on the mRNA expression of zinc transporters in adult rats
    Michael W Pfaffl
    Institute of Physiology, Freising Weihenstephan, Germany
    J Trace Elem Med Biol 17:97-106. 2003
  5. ncbi The gastrointestinal tract as target of steroid hormone action: quantification of steroid receptor mRNA expression (AR, ERalpha, ERbeta and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR
    M W Pfaffl
    Institute of Physiology, Research Centre for Milk and Food Weihenstephan, Weihenstephaner Berg 3, Technische Universitat Munchen, D 85350, Freising, Germany
    J Steroid Biochem Mol Biol 84:159-66. 2003
  6. ncbi Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: monitoring gene expression using cDNA microarrays combined with real-time RT-PCR
    Michael W Pfaffl
    Institute of Physiology, Department of Animal Sciences, Centre of Life and Food Sciences, Technical University of Munich, 85354 Freising, Germany
    J Nutr Biochem 14:691-702. 2003
  7. ncbi Development of clenbuterol reference materials: lyophilized bovine eye samples free of clenbuterol (CRM 673) and containing clenbuterol (CRM 674). Part 2: certification
    M W Pfaffl
    Technische Universitat Munchen, Institut fur Physiologie, Freising Weihenstephan, Germany
    Fresenius J Anal Chem 371:1092-7. 2001
  8. pmc Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    Michael W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 30:e36. 2002
  9. ncbi Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species
    M W Pfaffl
    Institute of Physiology, Center of Life and Food Sciences, Technical University of Munich, 85354 Freising Weihenstephan, Germany
    Domest Anim Endocrinol 22:91-102. 2002
  10. doi Identification of potential gene expression biomarkers for the surveillance of anabolic agents in bovine blood cells
    Irmgard Riedmaier
    Physiology Weihenstephan, Technische Universitaet Muenchen, Weihenstephaner Berg 3, 85354 Freising, Germany
    Anal Chim Acta 638:106-13. 2009

Collaborators

Detail Information

Publications45

  1. doi Changes in the miRNA profile under the influence of anabolic steroids in bovine liver
    Christiane Becker
    Physiology Weihenstephan, Technische Universitaet Muenchen, Weihenstephaner Berg 3, 85384 Freising, Germany
    Analyst 136:1204-9. 2011
    ..So, the combination of different transcribed targets (mRNA plus miRNA) might be a promising approach to find a valid expression pattern to be used for anabolic treatment screening...
  2. ncbi Development of clenbuterol reference materials: lyophilized bovine eye samples free of clenbuterol (CRM 673) and containing clenbuterol (CRM 674). Part 1. Preparation, homogeneity and stability
    M W Pfaffl
    Technische Universitat Munchen, Institut fur Physiologie, Freising Weihenstephan, Germany
    Fresenius J Anal Chem 371:1086-91. 2001
    ..Based on the results described above, it is concluded that both RMs may be suitable as candidates for certification...
  3. ncbi Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations
    Michael W Pfaffl
    Physiology, FML Weihenstephan, Centre of Life and Food Science, Technical University of Munich, Germany
    Biotechnol Lett 26:509-15. 2004
    ..The BestKeeper application and necessary information about data processing and handling can be downloaded on http://www.wzw.tum.de/gene-quantification/bestkeeper.html...
  4. ncbi Influence of zinc deficiency on the mRNA expression of zinc transporters in adult rats
    Michael W Pfaffl
    Institute of Physiology, Freising Weihenstephan, Germany
    J Trace Elem Med Biol 17:97-106. 2003
    ....
  5. ncbi The gastrointestinal tract as target of steroid hormone action: quantification of steroid receptor mRNA expression (AR, ERalpha, ERbeta and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR
    M W Pfaffl
    Institute of Physiology, Research Centre for Milk and Food Weihenstephan, Weihenstephaner Berg 3, Technische Universitat Munchen, D 85350, Freising, Germany
    J Steroid Biochem Mol Biol 84:159-66. 2003
    ..AR and PR seem to be not estrogen dependent...
  6. ncbi Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: monitoring gene expression using cDNA microarrays combined with real-time RT-PCR
    Michael W Pfaffl
    Institute of Physiology, Department of Animal Sciences, Centre of Life and Food Sciences, Technical University of Munich, 85354 Freising, Germany
    J Nutr Biochem 14:691-702. 2003
    ..Expression results imply that some genes are expressed constitutively, whereas others are highly regulated in tissues responsible for Zn homeostasis...
  7. ncbi Development of clenbuterol reference materials: lyophilized bovine eye samples free of clenbuterol (CRM 673) and containing clenbuterol (CRM 674). Part 2: certification
    M W Pfaffl
    Technische Universitat Munchen, Institut fur Physiologie, Freising Weihenstephan, Germany
    Fresenius J Anal Chem 371:1092-7. 2001
    ..5 microg kg(-1) and ca 10 microg kg(-1). This study has demonstrated that successful certification of clenbuterol-containing and clenbuterol-free bovine eye materials is possible...
  8. pmc Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    Michael W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 30:e36. 2002
    ..The latest software version of REST and examples for the correct use can be downloaded at http://www.wzw.tum.de/gene-quantification/...
  9. ncbi Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species
    M W Pfaffl
    Institute of Physiology, Center of Life and Food Sciences, Technical University of Munich, 85354 Freising Weihenstephan, Germany
    Domest Anim Endocrinol 22:91-102. 2002
    ....
  10. doi Identification of potential gene expression biomarkers for the surveillance of anabolic agents in bovine blood cells
    Irmgard Riedmaier
    Physiology Weihenstephan, Technische Universitaet Muenchen, Weihenstephaner Berg 3, 85354 Freising, Germany
    Anal Chim Acta 638:106-13. 2009
    ..Advanced statistical analysis by Principal Components Analysis (PCA) indicated that these genes represent potential biomarkers for this hormone combination in whole blood...
  11. doi Transcriptional biomarkers--high throughput screening, quantitative verification, and bioinformatical validation methods
    Irmgard Riedmaier
    Technische University Munich, Weihenstephaner Berg 3, 85354 Freising, Germany
    Methods 59:3-9. 2013
    ....
  12. doi RNA-sequencing as useful screening tool in the combat against the misuse of anabolic agents
    Irmgard Riedmaier
    Physiology Weihenstephan, and ZIEL Research Center for Nutrition and Food Sciences, Technical University Munich, Weihenstephaner Berg 3, 85354 Freising, Germany
    Anal Chem 84:6863-8. 2012
    ....
  13. doi Quality control for quantitative PCR based on amplification compatibility test
    Ales Tichopad
    Technical University Munich, Physiology Weihenstephan, Weihenstephaner Berg 3, 85354 Freising Weihenstephan, Germany
    Methods 50:308-12. 2010
    ..Finally we stress that the performance of the amplification compatibility test as a quality control procedure depends on the quality of the reference set...
  14. doi The physiological way: monitoring RNA expression changes as new approach to combat illegal growth promoter application
    Irmgard Riedmaier
    Physiology Weihenstephan, and ZIEL Research Center for Nutrition and Food Sciences, Technische University Munich, Weihenstephaner Berg 3, 85354, Freising, Germany
    Drug Test Anal 4:70-4. 2012
    ....
  15. ncbi Expression of estrogen and progesterone receptors in the bovine ovary during estrous cycle and pregnancy
    Bajram Berisha
    Institute of Physiology, Technical University Munich Weihenstephan, Freising, Germany
    Endocrine 17:207-14. 2002
    ..The results suggest an autocrine/paracrine role of steroid receptors in the regulation of final follicle growth and corpus luteum formation and function...
  16. doi Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses
    Andrea Hammerle-Fickinger
    Physiology Weihenstephan, Technische Universitaet Muenchen, Weihenstephaner Berg 3, 85354, Freising, Germany
    Biotechnol Lett 32:35-44. 2010
    ..The best microRNA qPCR results were obtained for samples extracted by the leukocyte extraction method...
  17. ncbi Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers
    Martina Reiter
    Physiology Weihenstephan, Technische Universitat Munchen, Weihenstephaner Berg 3, 85354 Freising, Germany
    Anal Chim Acta 586:73-81. 2007
    ..With the investigation of the regulation and possible function of anabolic sex steroids via gene expression, a preparatory work for the development of an expression pattern for drug screening was made...
  18. doi The analysis of the transcriptome as a new approach for biomarker development to trace the abuse of anabolic steroid hormones
    Irmgard Riedmaier
    Physiology Weihenstephan, Technische Universitaet Muenchen, Weihenstephaner Berg 3, 85354 Freising, Germany
    Drug Test Anal 3:676-81. 2011
    ..This review describes our recent efforts made concerning the analysis of gene expression changes in different tissues, different species and under different anabolic treatments...
  19. ncbi RNA integrity and the effect on the real-time qRT-PCR performance
    Simone Fleige
    Physiology Weihenstephan, Center of Life and Food Sciences ZIEL, Technical University of Munich, 85350 Freising, Germany
    Mol Aspects Med 27:126-39. 2006
    ..We can recommend a RIN higher than five as good total RNA quality and higher than eight as perfect total RNA for downstream application...
  20. doi The use of omic technologies for biomarker development to trace functions of anabolic agents
    Irmgard Riedmaier
    Physiology Weihenstephan, Technische Universitaet Muenchen, Weihenstephaner Berg 3, 85354 Freising, Germany
    J Chromatogr A 1216:8192-9. 2009
    ..This review describes the potential of these omic technologies for the development of such new screening methods and presents recent literature in this field...
  21. ncbi Inhibition of real-time RT-PCR quantification due to tissue-specific contaminants
    Ales Tichopad
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Science, Technical University of Munich, Germany
    Mol Cell Probes 18:45-50. 2004
    ..We observed that unknown tissue-specific factors can influence amplification kinetics but this affect can be ameliorated, in part, by appropriate primer selection...
  22. pmc Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs--a comparative study
    Swanhild U Meyer
    Physiology Weihenstephan, ZIEL Research Center for Nutrition and Food Sciences, Technische Universitat Munchen, Freising, Germany
    PLoS ONE 7:e38946. 2012
    ....
  23. doi Influence of testosterone and a novel SARM on gene expression in whole blood of Macaca fascicularis
    Irmgard Riedmaier
    Physiology Weihenstephan, Technische Universitat Munchen, Weihenstephaner Berg 3, Freising, Germany
    J Steroid Biochem Mol Biol 114:167-73. 2009
    ....
  24. ncbi Effects of varied EGCG and (+)-catechin concentrations on proinflammatory cytokines mrna expression in cona-stimulated primary white blood cell cultures
    Julia Sehm
    Lehrstuhl für Physiologie Weihenstephan, Zentralinstitut für Ernährung und Lebensmittelforschung, Technische Universitat Munchen, Freising, Germany
    J Agric Food Chem 53:6907-11. 2005
    ..It is presumed that both flavanoids have the potential to regulate total RNA expression and gene-specific expression in WBC...
  25. doi Expression of immune relevant genes in pigs under the influence of low doses of deoxynivalenol (DON)
    Christiane Becker
    Animal Hygiene, Technische Universitaet Muenchen, Weihenstephaner Berg 3, Freising Weihenstephan, Germany
    Mycotoxin Res 27:287-93. 2011
    ..Down-regulations of immune-related transcription factors and pro-inflammatory immune factors could be demonstrated...
  26. doi Normalization strategies for microRNA profiling experiments: a 'normal' way to a hidden layer of complexity?
    Swanhild U Meyer
    Physiology Weihenstephan, ZIEL Research Center for Nutrition and Food Sciences, Technische Universitat Munchen, Weihenstephaner Berg 3, 85354, Freising, Germany
    Biotechnol Lett 32:1777-88. 2010
    ..Emphasis is laid on the complexity, the importance and the potential for further optimization of normalization techniques for miRNA profiling datasets...
  27. ncbi Tissue-specific expression pattern of bovine prion gene: quantification using real-time RT-PCR
    Ales Tichopad
    Institute of Physiology, FML Weihenstephan, Technical University of Munich, Weihenstephaner Berg 3, 85354 Freising, Germany
    Mol Cell Probes 17:5-10. 2003
    ..LightCycler technology is currently considered the most precise method for nucleic acid quantification and showed to be powerful tool for further studies on prion diseases pathogenesis...
  28. pmc Standardized determination of real-time PCR efficiency from a single reaction set-up
    Ales Tichopad
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Science, Technical University of Munich, Germany
    Nucleic Acids Res 31:e122. 2003
    ..Furthermore, it resists the subjective influence of researchers, and the estimation can therefore be fully instrumentalized...
  29. ncbi Effects of increased cellular zinc levels on gene and protein expression in HT-29 cells
    Birgit Kindermann
    Molecular Nutrition Unit, Technical University of Munich, 85350 Freising Weihenstephan, Germany
    Biometals 18:243-53. 2005
    ..More than half of the targets participate in ATP production or stress response. Therefore, it appears that higher zinc concentrations mediate their effects mainly via impairments in cellular energy metabolism and stress response...
  30. doi Long-term effects of mycophenolic acid on the immunoglobulin and inflammatory marker-gene expression in sheep white blood cells
    Anamarija Dzidic
    Physiology Weihenstephan, Center of Life and Food Science, Technical University of Munich, Weihenstephaner Berg 3, 85354, Freising, Germany
    Mycotoxin Res 26:235-40. 2010
    ..According to our data, oral uptake of silage extremely contaminated with a very high concentration of MPA did not have a significant influence on immunoglobulins and cytokines in WBC...
  31. ncbi Non-invasive screening for treatment of heifers with the anabolic steroid melengestrol acetate (MGA) by feces analysis
    Iris G Lange
    Institut fur Physiologie, TU München Weihenstephan, Weihenstephaner, Freising Weihenstephan, Germany
    J Immunoassay Immunochem 24:265-72. 2003
    ..In contrast to urine analysis, feces analysis appeared to be a suitable means of non-invasive screening before slaughter for surveillance of MGA treatment and verification of its compliance with labeled dosage...
  32. ncbi Model of inhibition of Thermus aquaticus polymerase and Moloney murine leukemia virus reverse transcriptase by tea polyphenols (+)-catechin and (-)-epigallocatechin-3-gallate
    Ales Tichopad
    Physiology Weihenstephan, Zentralinstitut für Ernährung und Lebensmittel forschung, Technische Universitat Munchen, D 85350 Freising, Germany
    J Ethnopharmacol 99:221-7. 2005
    ..001). Similarly, (+)-catechin inhibited the DNA amplification (p < 0.01) but had no effect on the RT reaction. The effects could be observed in physiological flavanol concentrations ranging from 10(-5) to 10(-8)M...
  33. pmc Synergetic downregulation of 67 kDa laminin receptor by the green tea (Camellia sinensis) secondary plant compound epigallocatechin gallate: a new gateway in metastasis prevention?
    Jakob Müller
    Physiology Weihenstephan, Technische Universitat Munchen, Research Center for Nutrition and Food Science, Weihenstephaner Berg 3, 85350 Freising, Germany
    BMC Complement Altern Med 12:258. 2012
    ..This study investigated the role of the major tea secondary plant compound epigallocatechin gallate (EGCG) for its inhibitory effects on the metastasis-associated 67 kDa laminin receptor (67LR)...
  34. ncbi Effects of lactoferrin feeding on growth, feed intake and health of calves
    Maria L Prenner
    Department of Animal Sciences, Technische Universität München Weihenstephan, Germany
    Arch Anim Nutr 61:20-30. 2007
    ..This study indicates that LF is advantageous for health and may therefore be a beneficial supplement in the diets for neonatal calves...
  35. ncbi Effects of mycophenolic acid (MPA) treatment on expression of Fc receptor (FcRn) and polymeric immunoglobulin receptor (pIgR) mRNA in adult sheep tissues
    Anamarija Dzidic
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Science, Technical University of Munich, Weihenstephaner, Freising Weihenstephan, Germany
    Croat Med J 45:130-5. 2004
    ....
  36. ncbi Identification of a potential gene expression biomarker signature in bovine liver to detect the abuse of growth promoters
    Irmgard Riedmaier
    a Physiology Weihenstephan, and ZIEL Research Center for Nutrition and Food Sciences, Technical University Munich, Freising, Germany
    Food Addit Contam Part A Chem Anal Control Expo Risk Assess 31:641-9. 2014
    ....
  37. ncbi mRNA and microRNA purity and integrity: the key to success in expression profiling
    Benedikt Kirchner
    Physiology Weihenstephan, ZIEL Research Center for Nutrition and Food Sciences Technische Universität München, Weihenstephaner Berg 3, D 85354, Freising, Germany
    Methods Mol Biol 1160:43-53. 2014
    ..Herein we describe the current state-of-the-art RNA quality assessment by combining UV/Vis spectrophotometry and microfluidic capillary electrophoresis. ..
  38. ncbi Posttranscriptional regulatory networks: from expression profiling to integrative analysis of mRNA and microRNA data
    Swanhild U Meyer
    Physiology Weihenstephan, ZIEL Research Center for Nutrition and Food Sciences, Technische Universitat Munchen, Weihenstephaner Berg 3, D 85354, Freising, Germany
    Methods Mol Biol 1160:165-88. 2014
    ....
  39. doi Electric cell-substrate impedance sensing (ECIS) based real-time measurement of titer dependent cytotoxicity induced by adenoviral vectors in an IPI-2I cell culture model
    Jakob Müller
    Physiology Unit, Technische Universitat Munchen, Research Center for Nutrition and Food Science, Weihenstephaner Berg 3, 85350 Freising Weihenstephan, Germany
    Biosens Bioelectron 26:2000-5. 2011
    ..In addition this work suggests that the ECIS-device bears the feasibility to transfer this assay to multiple other cytotoxicological questions...
  40. ncbi A method to assess 59Fe in residual tissue blood content in mice and its use to correct 59Fe-distribution kinetics accordingly
    Klaus Schümann
    Wissenschaftszentrum Weihenstephan, Technische Universitat Munchen, Germany
    Toxicology 241:19-32. 2007
    ..To investigate the molecular mechanisms of iron homeostasis, corresponding essential genes are manipulated by many working groups. This asks for a simple method to pursue the resulting impact on body iron-distribution in mice...
  41. ncbi Quantitative mRNA analysis of eight bovine 5-HT receptor subtypes in brain, abomasum, and intestine by real-time RT-PCR
    Martin Reist
    Department of Clinical Veterinary Medicine, Clinic for Ruminants, Faculty of Veterinary Medicine, University of Berne, Berne, Switzerland
    J Recept Signal Transduct Res 23:271-87. 2003
    ..In conclusion, accurate real-time RT-PCR assays for quantitative analysis of bovine 5-HTR subtype gene expression were developed and validated...
  42. ncbi Elevated caspase-3 and Fas mRNA expression in jejunum of adult rats during subclinical zinc deficiency
    Andrea Didier
    Institute of Hygiene and Technology of Milk, Faculty of Veterinary Medicine, Ludwig Maximilian University, Schönleutnerstrasse 8, 85674 Oberschleissheim, Germany
    J Trace Elem Med Biol 18:41-5. 2004
    ..This study provides the first evidence that even moderate zinc deficiency in an adult, non-growing rat model is able to elevate mRNA expression levels of factors involved in early stages of apoptosis...
  43. ncbi Distribution-insensitive cluster analysis in SAS on real-time PCR gene expression data of steadily expressed genes
    Ales Tichopad
    IMFORM GmBH, International Clinical Research, Darmstadt, Germany
    Comput Methods Programs Biomed 82:44-50. 2006
    ..Simulated data in parallel with the data from a biological experiment were taken to validate the SAS macro. For both cases, good intuitive results were obtained...
  44. ncbi Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR
    Simone Fleige
    Physiology Weihenstephan, Center of Life and Food Sciences ZIEL, Technical University Munich, Germany
    Biotechnol Lett 28:1601-13. 2006
    ..Results demonstrate that innovative new quantification methods and normalization models can improve future mRNA quantification...
  45. ncbi Differential expression of molecular motors in the motor cortex of sporadic ALS
    Maria Pantelidou
    Department of Biological Sciences, University of Cyprus and Cyprus Institute of Neurology and Genetics, P O Box 20537, 1678 Nicosia, Cyprus
    Neurobiol Dis 26:577-89. 2007
    ..No mutations causing splicing defects or altering protein-coding sequences were identified in the genes of the three proteins. The present study implicates two motor proteins as possible candidates in SALS pathology...