Reiner Peters

Summary

Country: Germany

Publications

  1. ncbi request reprint The nanopore connection to cell membrane unitary permeability
    Reiner Peters
    Institute of Medical Physics and Biophysics, University of Muenster, Robert Koch Strasse 31, 48149 Munster, Germany, Center of Nanotechnology, Muenster, Germany
    Traffic 6:199-204. 2005
  2. pmc Line-scanning microphotolysis for diffraction-limited measurements of lateral diffusion
    P Wedekind
    Institut fur Medizinische Physik und Biophysik, Westfalische Wilhelms Universitat, Munster, Germany
    Biophys J 71:1621-32. 1996
  3. ncbi request reprint Translocation through the nuclear pore complex: selectivity and speed by reduction-of-dimensionality
    Reiner Peters
    Institute of Medical Physics and Biophysics and Center of Nanotechnology CeNTech, University of Muenster, Robert Koch Strasse 31, 48149 Muenster, Germany
    Traffic 6:421-7. 2005
  4. ncbi request reprint Optical single transporter recording: transport kinetics in microarrays of membrane patches
    Reiner Peters
    Institut fur Medizinische Physik und Biophysik, Universitat Munster, Munster, Germany
    Annu Rev Biophys Biomol Struct 32:47-67. 2003
  5. doi request reprint Binding site distribution of nuclear transport receptors and transport complexes in single nuclear pore complexes
    Martin Kahms
    Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Munster, 48149 Munster, Germany
    Traffic 10:1228-42. 2009
  6. pmc Nuclear transport of single molecules: dwell times at the nuclear pore complex
    Ulrich Kubitscheck
    Institute of Medical Physics and Biophysics, Westfalische Wilhelms Universitat, D 48149 Munster, Germany
    J Cell Biol 168:233-43. 2005
  7. ncbi request reprint Nuclear transport kinetics in microarrays of nuclear envelope patches
    Reiner Peters
    Institut fur Medizinische Physik und Biophysik, Universitat Munster, Robert Koch Strasse 31, Munster, Germany
    J Struct Biol 140:268-78. 2002
  8. ncbi request reprint Single-molecule fluorescence analysis of cellular nanomachinery components
    Reiner Peters
    Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Munster, 48149 Munster, Germany
    Annu Rev Biophys Biomol Struct 36:371-94. 2007
  9. doi request reprint Lighting up the nuclear pore complex
    Martin Kahms
    Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Muenster, Heisenbergstrasse 11, 48149 Muenster, Germany
    Eur J Cell Biol 90:751-8. 2011
  10. pmc Visualization and tracking of single protein molecules in the cell nucleus
    T Kues
    , , , Germany
    Biophys J 80:2954-67. 2001

Collaborators

  • KLAUS J SCHULTEN
  • Toshihiro Sekimoto
  • DAVID GRUNWALD
  • U Kubitscheck
  • Y Yoneda
  • Klemens Wild
  • Martin Kahms
  • Jana Hüve
  • Jan Peter Siebrasse
  • T Kues
  • Ramona Wesselmann
  • Viola Baumgärtel
  • Bettina Bradatsch
  • Anton Arkhipov
  • Roland Hemmler
  • Nikolai I Kiskin
  • Maret Böhm
  • T Weide
  • T Radtke
  • Julia C Farr
  • Nils Sanetra
  • Philipp Lehrich
  • Jochen Bassler
  • Ed Hurt
  • Eva Kowalinski
  • Wei Yao
  • Jun Katahira
  • Guido Boese
  • Irmi Sinning
  • Gert Bange
  • Richard Wagner
  • Guido Böse
  • Karl Heinz Klempnauer
  • Jan P Siebrasse
  • Kirsty Sawicka
  • Anne Brehmer-Fastnacht
  • P Wedekind
  • Elias Coutavas
  • A Dickmanns
  • E Coutavas
  • D Schmalz
  • T M Soliman
  • A Barnekow
  • M Koster
  • M Bayer
  • J P Siebrasse
  • M Grote
  • O Heinrich
  • R Reichelt

Detail Information

Publications28

  1. ncbi request reprint The nanopore connection to cell membrane unitary permeability
    Reiner Peters
    Institute of Medical Physics and Biophysics, University of Muenster, Robert Koch Strasse 31, 48149 Munster, Germany, Center of Nanotechnology, Muenster, Germany
    Traffic 6:199-204. 2005
    ..Finally, prospects for combining OSTR with 4Pi microscopy, single-molecule fluorescence spectroscopy and fluorescence correlation spectroscopy are discussed...
  2. pmc Line-scanning microphotolysis for diffraction-limited measurements of lateral diffusion
    P Wedekind
    Institut fur Medizinische Physik und Biophysik, Westfalische Wilhelms Universitat, Munster, Germany
    Biophys J 71:1621-32. 1996
    ..A first application of the method to the diffusion of a fluorescently labeled tracer inside the cell nucleus showed the potential of the method for the study of complex biological systems...
  3. ncbi request reprint Translocation through the nuclear pore complex: selectivity and speed by reduction-of-dimensionality
    Reiner Peters
    Institute of Medical Physics and Biophysics and Center of Nanotechnology CeNTech, University of Muenster, Robert Koch Strasse 31, 48149 Muenster, Germany
    Traffic 6:421-7. 2005
    ..The model features virtual gating, is compatible with but not dependent on FG affinity gradients and tolerates deletions and transpositions of FG motifs. Implications of the model are discussed and tests are suggested...
  4. ncbi request reprint Optical single transporter recording: transport kinetics in microarrays of membrane patches
    Reiner Peters
    Institut fur Medizinische Physik und Biophysik, Universitat Munster, Munster, Germany
    Annu Rev Biophys Biomol Struct 32:47-67. 2003
    ..Applications to nuclear transport are summarized. The further development of OSTR is discussed and its extension to a diversity of transporters, including translocases and ATP-binding cassette (ABC) pumps, projected...
  5. doi request reprint Binding site distribution of nuclear transport receptors and transport complexes in single nuclear pore complexes
    Martin Kahms
    Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Munster, 48149 Munster, Germany
    Traffic 10:1228-42. 2009
    ....
  6. pmc Nuclear transport of single molecules: dwell times at the nuclear pore complex
    Ulrich Kubitscheck
    Institute of Medical Physics and Biophysics, Westfalische Wilhelms Universitat, D 48149 Munster, Germany
    J Cell Biol 168:233-43. 2005
    ..Together with the known transport rates, our data suggest that nucleocytoplasmic transport occurs via multiple parallel pathways within single NPCs...
  7. ncbi request reprint Nuclear transport kinetics in microarrays of nuclear envelope patches
    Reiner Peters
    Institut fur Medizinische Physik und Biophysik, Universitat Munster, Robert Koch Strasse 31, Munster, Germany
    J Struct Biol 140:268-78. 2002
    ..This opens new avenues to the analysis of nuclear transport including the export of nucleic-acid-protein and ribosomal particles...
  8. ncbi request reprint Single-molecule fluorescence analysis of cellular nanomachinery components
    Reiner Peters
    Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Munster, 48149 Munster, Germany
    Annu Rev Biophys Biomol Struct 36:371-94. 2007
    ..We discuss how current limitations in the nanoscopic analysis of living cells and tissues can be overcome to create a new paradigm, nanoscopic biomedicine...
  9. doi request reprint Lighting up the nuclear pore complex
    Martin Kahms
    Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Muenster, Heisenbergstrasse 11, 48149 Muenster, Germany
    Eur J Cell Biol 90:751-8. 2011
    ....
  10. pmc Visualization and tracking of single protein molecules in the cell nucleus
    T Kues
    , , , Germany
    Biophys J 80:2954-67. 2001
    ..However, intranuclear protein diffusion is severely restricted, most likely by multiple association-dissociation events and/or impermeable obstacles...
  11. pmc Imaging and tracking of single GFP molecules in solution
    U Kubitscheck
    Institut fur Medizinische Physik und Biophysik, Westfalische Wilhelms Universitat, Robert Koch Strasse 31, D 48149 Munster, Germany
    Biophys J 78:2170-9. 2000
    ..Our results imply that the visualization and tracking of single molecules in living cells is possible...
  12. pmc Reconstitution of nuclear protein export in isolated nuclear envelopes
    Jan Peter Siebrasse
    Institut fur Medizinische Physik und Biophysik, Universitat Munster, 48149 Munster, Germany
    J Cell Biol 158:849-54. 2002
    ..This paper shows that nuclear transport, previously studied in intact and permeabilized cells only, can be quantitatively analyzed in perforated nuclei and isolated nuclear envelopes...
  13. ncbi request reprint Nanoscopic medicine: the next frontier
    Reiner Peters
    Institute of Medical Physics and Biophysics, University of Munster, Robert Koch Strasse 31, 48149 Munster, Germany
    Small 2:452-6. 2006
    ..It aims at the diagnosis and therapy of diseases by directly visualizing, analyzing, and modifying NPMs and their networks in living cells and tissues...
  14. ncbi request reprint Checking and fixing the cellular nanomachinery: towards medical nanoscopy
    Reiner Peters
    Institute of Medical Physics and Biophysics, and Center of Nanotechnology CeNTech, University of Muenster, 48149 Germany
    Trends Mol Med 12:83-9. 2006
    ..Together, progress in functional proteomics and live-cell imaging provide novel possibilities for directly analyzing and modifying nanoscopic protein machines in living cells and tissues...
  15. pmc High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking
    T Kues
    , , Robert-Koch-Strasse 31, , Germany
    Proc Natl Acad Sci U S A 98:12021-6. 2001
    ..The results substantiate the view that nuclear speckles are not rigid structures but highly dynamic domains characterized by a rapid turnover of U1 snRNPs and other splicing factors...
  16. pmc 4Pi microscopy of the nuclear pore complex
    Jana Hüve
    Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Munster, Munster, Germany
    Biophys J 95:877-85. 2008
    ..We conclude that super-resolution fluorescence microscopy is a powerful method for analyzing single protein complexes and the cellular nanomachinery in general...
  17. pmc Kinetics of protein import into isolated Xenopus oocyte nuclei
    T Radtke
    , , Robert-Koch-Strasse 31, , Germany
    Proc Natl Acad Sci U S A 98:2407-12. 2001
    ....
  18. ncbi request reprint Mapping of nucleoporins to the center of the nuclear pore complex by post-embedding immunogold electron microscopy
    M Grote
    Institut fur Medizinische Physik und Biophysik, Westfalische Wilhelms Universitat, Munster, Germany
    J Cell Sci 108:2963-72. 1995
    ..Our experimental approach was verified by mapping gp210, another nuclear pore protein, at or very close to the NPC in the perinuclear cisterna thus establishing a distribution pattern completely different from that of the nucleoporins...
  19. pmc The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b
    T Weide
    Department of Experimental Tumorbiology, University of Muenster, Badestrasse 9, D 48149 Muenster, Germany
    EMBO Rep 2:336-41. 2001
    ..We mapped the rab1b binding site of GM130 and provide evidence that it is different to the previously described p115 and Grasp65 binding sites of the GM130 protein...
  20. pmc Rapid translocation of NTF2 through the nuclear pore of isolated nuclei and nuclear envelopes
    Jan Peter Siebrasse
    Institut fur Medizinische Physik und Biophysik, Universitat Munster, Germany
    EMBO Rep 3:887-92. 2002
    ....
  21. ncbi request reprint The transformation suppressor protein Pdcd4 shuttles between nucleus and cytoplasm and binds RNA
    Maret Böhm
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Münster1, Germany
    Oncogene 22:4905-10. 2003
    ..Taken together, our data raise the possibility that Pdcd4 is involved in some aspect of nuclear RNA metabolism in addition to its suspected role in protein translation...
  22. ncbi request reprint Arx1 functions as an unorthodox nuclear export receptor for the 60S preribosomal subunit
    Bettina Bradatsch
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Mol Cell 27:767-79. 2007
    ..In contrast, the predicted central cavity of Arx1 is involved in the interaction with FG repeat nucleoporins and 60S subunit export. Thus, an ancient enzyme fold has been adopted by Arx1 to function as a nuclear export receptor...
  23. pmc Continuous fluorescence microphotolysis and correlation spectroscopy using 4Pi microscopy
    Anton Arkhipov
    Department of Physics and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    Biophys J 93:4006-17. 2007
    ..In all cases experimental data could be well fitted by computed curves for expected diffusion coefficients, even when the signal/noise ratio was small due to the small number of fluorophores involved...
  24. pmc Optical microwell assay of membrane transport kinetics
    Nikolai I Kiskin
    Institut fur Medizinische Physik und Biophysik, Westfalische Wilhelms Universitat, D 48149 Munster, Germany
    Biophys J 85:2311-22. 2003
    ..Unexpectedly, NXT1, a homolog of NTF2 reportedly unable to bind to NPC proteins directly, was translocated as fast as NTF2. Thus, microstructured TC arrays were shown to provide optical single transporter recording with a new basis...
  25. pmc Nanopore unitary permeability measured by electrochemical and optical single transporter recording
    Roland Hemmler
    Institut fur Medizinische Physik und Biophysik, Universitat Munster, Germany
    Biophys J 88:4000-7. 2005
    ....
  26. ncbi request reprint Use of Xenopus laevis oocyte nuclei and nuclear envelopes in nucleocytoplasmic transport studies
    Reiner Peters
    Institute of Medical Physics and Biophysics and Center for Nanotechnology, University of Munster, Germany
    Methods Mol Biol 322:259-72. 2006
    ..Transport through membrane patches spanning these cavities is recorded by confocal microscopy. By these means, the transport through single nuclear pore complexes or populations of pore complexes can be quantitatively measured...
  27. ncbi request reprint Introduction to nucleocytoplasmic transport: molecules and mechanisms
    Reiner Peters
    Institute of Medical Physics and Biophysics and Center for Nanotechnology, University of Munster, Germany
    Methods Mol Biol 322:235-58. 2006
    ..Finally, the still essentially unresolved mechanisms by which nuclear transport receptors and transport complexes are translocated through the nuclear pore complex are considered, and a novel translocation model is suggested...
  28. doi request reprint From fluorescence nanoscopy to nanoscopic medicine
    Reiner Peters
    Nanomedicine (Lond) 3:1-4. 2008