Research Topics
Species | Reiner PetersSummaryCountry: Germany Publications
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Publications
The nanopore connection to cell membrane unitary permeabilityReiner Peters
Institute of Medical Physics and Biophysics, University of Muenster, Robert Koch Strasse 31, 48149 Munster, Germany, Center of Nanotechnology, Muenster, Germany
Traffic 6:199-204. 2005..Finally, prospects for combining OSTR with 4Pi microscopy, single-molecule fluorescence spectroscopy and fluorescence correlation spectroscopy are discussed...- Line-scanning microphotolysis for diffraction-limited measurements of lateral diffusionP Wedekind
Institut fur Medizinische Physik und Biophysik, Westfalische Wilhelms Universitat, Munster, Germany
Biophys J 71:1621-32. 1996..A first application of the method to the diffusion of a fluorescently labeled tracer inside the cell nucleus showed the potential of the method for the study of complex biological systems... - Optical single transporter recording: transport kinetics in microarrays of membrane patchesReiner Peters
Institut fur Medizinische Physik und Biophysik, Universitat Munster, Munster, Germany
Annu Rev Biophys Biomol Struct 32:47-67. 2003..Applications to nuclear transport are summarized. The further development of OSTR is discussed and its extension to a diversity of transporters, including translocases and ATP-binding cassette (ABC) pumps, projected... - Translocation through the nuclear pore complex: selectivity and speed by reduction-of-dimensionalityReiner Peters
Institute of Medical Physics and Biophysics and Center of Nanotechnology CeNTech, University of Muenster, Robert Koch Strasse 31, 48149 Muenster, Germany
Traffic 6:421-7. 2005..The model features virtual gating, is compatible with but not dependent on FG affinity gradients and tolerates deletions and transpositions of FG motifs. Implications of the model are discussed and tests are suggested... - Binding site distribution of nuclear transport receptors and transport complexes in single nuclear pore complexesMartin Kahms
Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Munster, 48149 Munster, Germany
Traffic 10:1228-42. 2009.... - Nuclear transport kinetics in microarrays of nuclear envelope patchesReiner Peters
Institut fur Medizinische Physik und Biophysik, Universitat Munster, Robert Koch Strasse 31, Munster, Germany
J Struct Biol 140:268-78. 2002..This opens new avenues to the analysis of nuclear transport including the export of nucleic-acid-protein and ribosomal particles... - Nuclear transport of single molecules: dwell times at the nuclear pore complexUlrich Kubitscheck
Institute of Medical Physics and Biophysics, Westfalische Wilhelms Universitat, D 48149 Munster, Germany
J Cell Biol 168:233-43. 2005..Together with the known transport rates, our data suggest that nucleocytoplasmic transport occurs via multiple parallel pathways within single NPCs... - Single-molecule fluorescence analysis of cellular nanomachinery componentsReiner Peters
Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Munster, 48149 Munster, Germany
Annu Rev Biophys Biomol Struct 36:371-94. 2007..We discuss how current limitations in the nanoscopic analysis of living cells and tissues can be overcome to create a new paradigm, nanoscopic biomedicine... - Visualization and tracking of single protein molecules in the cell nucleusT Kues
, , , Germany
Biophys J 80:2954-67. 2001..However, intranuclear protein diffusion is severely restricted, most likely by multiple association-dissociation events and/or impermeable obstacles... - Imaging and tracking of single GFP molecules in solutionU Kubitscheck
Institut fur Medizinische Physik und Biophysik, Westfalische Wilhelms Universitat, Robert Koch Strasse 31, D 48149 Munster, Germany
Biophys J 78:2170-9. 2000..Our results imply that the visualization and tracking of single molecules in living cells is possible... - Checking and fixing the cellular nanomachinery: towards medical nanoscopyReiner Peters
Institute of Medical Physics and Biophysics, and Center of Nanotechnology CeNTech, University of Muenster, 48149 Germany
Trends Mol Med 12:83-9. 2006..Together, progress in functional proteomics and live-cell imaging provide novel possibilities for directly analyzing and modifying nanoscopic protein machines in living cells and tissues... - Lighting up the nuclear pore complexMartin Kahms
Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Muenster, Heisenbergstrasse 11, 48149 Muenster, Germany
Eur J Cell Biol 90:751-8. 2011.... - Nanoscopic medicine: the next frontierReiner Peters
Institute of Medical Physics and Biophysics, University of Munster, Robert Koch Strasse 31, 48149 Munster, Germany
Small 2:452-6. 2006..It aims at the diagnosis and therapy of diseases by directly visualizing, analyzing, and modifying NPMs and their networks in living cells and tissues... - Reconstitution of nuclear protein export in isolated nuclear envelopesJan Peter Siebrasse
Institut fur Medizinische Physik und Biophysik, Universitat Munster, 48149 Munster, Germany
J Cell Biol 158:849-54. 2002..This paper shows that nuclear transport, previously studied in intact and permeabilized cells only, can be quantitatively analyzed in perforated nuclei and isolated nuclear envelopes... - High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle trackingT Kues
, , Robert-Koch-Strasse 31, , Germany
Proc Natl Acad Sci U S A 98:12021-6. 2001..The results substantiate the view that nuclear speckles are not rigid structures but highly dynamic domains characterized by a rapid turnover of U1 snRNPs and other splicing factors... - 4Pi microscopy of the nuclear pore complexJana Hüve
Institute of Medical Physics and Biophysics, and Center for Nanotechnology CeNTech, University of Munster, Munster, Germany
Biophys J 95:877-85. 2008..We conclude that super-resolution fluorescence microscopy is a powerful method for analyzing single protein complexes and the cellular nanomachinery in general... - Kinetics of protein import into isolated Xenopus oocyte nucleiT Radtke
, , Robert-Koch-Strasse 31, , Germany
Proc Natl Acad Sci U S A 98:2407-12. 2001.... - The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1bT Weide
Department of Experimental Tumorbiology, University of Muenster, Badestrasse 9, D 48149 Muenster, Germany
EMBO Rep 2:336-41. 2001..We mapped the rab1b binding site of GM130 and provide evidence that it is different to the previously described p115 and Grasp65 binding sites of the GM130 protein... - Mapping of nucleoporins to the center of the nuclear pore complex by post-embedding immunogold electron microscopyM Grote
Institut fur Medizinische Physik und Biophysik, Westfalische Wilhelms Universitat, Munster, Germany
J Cell Sci 108:2963-72. 1995..Our experimental approach was verified by mapping gp210, another nuclear pore protein, at or very close to the NPC in the perinuclear cisterna thus establishing a distribution pattern completely different from that of the nucleoporins... - Use of Xenopus laevis oocyte nuclei and nuclear envelopes in nucleocytoplasmic transport studiesReiner Peters
Institute of Medical Physics and Biophysics and Center for Nanotechnology, , Germany
Methods Mol Biol 322:259-72. 2006..Transport through membrane patches spanning these cavities is recorded by confocal microscopy. By these means, the transport through single nuclear pore complexes or populations of pore complexes can be quantitatively measured... - Introduction to nucleocytoplasmic transport: molecules and mechanismsReiner Peters
Institute of Medical Physics and Biophysics and Center for Nanotechnology, , Germany
Methods Mol Biol 322:235-58. 2006..Finally, the still essentially unresolved mechanisms by which nuclear transport receptors and transport complexes are translocated through the nuclear pore complex are considered, and a novel translocation model is suggested... - Rapid translocation of NTF2 through the nuclear pore of isolated nuclei and nuclear envelopesJan Peter Siebrasse
, , Germany
EMBO Rep 3:887-92. 2002.... - Continuous fluorescence microphotolysis and correlation spectroscopy using 4Pi microscopyAnton Arkhipov
Department of Physics and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
Biophys J 93:4006-17. 2007..In all cases experimental data could be well fitted by computed curves for expected diffusion coefficients, even when the signal/noise ratio was small due to the small number of fluorophores involved... - Arx1 functions as an unorthodox nuclear export receptor for the 60S preribosomal subunitBettina Bradatsch
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Mol Cell 27:767-79. 2007..In contrast, the predicted central cavity of Arx1 is involved in the interaction with FG repeat nucleoporins and 60S subunit export. Thus, an ancient enzyme fold has been adopted by Arx1 to function as a nuclear export receptor... - Nanopore unitary permeability measured by electrochemical and optical single transporter recordingRoland Hemmler
, , Germany
Biophys J 88:4000-7. 2005.... - The transformation suppressor protein Pdcd4 shuttles between nucleus and cytoplasm and binds RNAMaret Böhm
Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Münster1, Germany
Oncogene 22:4905-10. 2003..Taken together, our data raise the possibility that Pdcd4 is involved in some aspect of nuclear RNA metabolism in addition to its suspected role in protein translation... - Optical microwell assay of membrane transport kineticsNikolai I Kiskin
, , , Germany
Biophys J 85:2311-22. 2003..Unexpectedly, NXT1, a homolog of NTF2 reportedly unable to bind to NPC proteins directly, was translocated as fast as NTF2. Thus, microstructured TC arrays were shown to provide optical single transporter recording with a new basis... - From fluorescence nanoscopy to nanoscopic medicineReiner Peters
Nanomed 3:1-4. 2008