S Pascolo

Summary

Country: Germany

Publications

  1. pmc Identification of thymus specific and developmentally regulated genes by an improved version of the mRNA differential display technique
    S Pascolo
    Interfakultäres Institut für Zellbiologie, Abt Immunologie Auf der Morgenstelle 15, Tubingen, Germany
    Dev Immunol 7:1-7. 1999
  2. ncbi request reprint The non-classical HLA class I molecule HFE does not influence the NK-like activity contained in fresh human PBMCs and does not interact with NK cells
    Steve Pascolo
    Department of Immunology, Auf der Morgenstelle 15, 72076 Tubingen, Germany
    Int Immunol 17:117-22. 2005
  3. ncbi request reprint HLA class I transgenic mice: development, utilisation and improvement
    Steve Pascolo
    Department of Immunology, University of Tubingen, Auf der Morgenstelle 15, 72076 Tubingen, Germany
    Expert Opin Biol Ther 5:919-38. 2005
  4. ncbi request reprint Plasmid DNA- and messenger RNA-based anti-cancer vaccination
    Benjamin Weide
    Department of Dermatology, University of Tubingen, Liebermeisterstrasse 25, Tubingen, Germany
    Immunol Lett 115:33-42. 2008
  5. ncbi request reprint A MAGE-A1 HLA-A A*0201 epitope identified by mass spectrometry
    S Pascolo
    Institut for Cell Biology, Department Immunology, University of Tubingen, Auf der Morgenstelle 15, 72076 Tubingen, Germany
    Cancer Res 61:4072-7. 2001
  6. doi request reprint Results of the first phase I/II clinical vaccination trial with direct injection of mRNA
    Benjamin Weide
    Department of Dermatology, Institute for Cell Biology, University of Tubingen, Germany
    J Immunother 31:180-8. 2008
  7. ncbi request reprint Spontaneous cellular uptake of exogenous messenger RNA in vivo is nucleic acid-specific, saturable and ion dependent
    J Probst
    Department of Immunology, Institute for Cell Biology, University of Tubingen, Tubingen, Germany
    Gene Ther 14:1175-80. 2007
  8. ncbi request reprint Human peripheral blood mononuclear cells transfected with messenger RNA stimulate antigen-specific cytotoxic T-lymphocytes in vitro
    R Teufel
    CureVac GmbH, Paul Ehrlich Str 15, 72076, Tubingen, Germany
    Cell Mol Life Sci 62:1755-62. 2005
  9. doi request reprint Direct injection of protamine-protected mRNA: results of a phase 1/2 vaccination trial in metastatic melanoma patients
    Benjamin Weide
    Department of Dermatology, Division of Dermatooncology, University of Tubingen, Tubingen, Germany
    J Immunother 32:498-507. 2009
  10. doi request reprint Novel multi-peptide vaccination in Hla-A2+ hormone sensitive patients with biochemical relapse of prostate cancer
    Susan Feyerabend
    Department of Urology, University of Tubingen, Germany
    Prostate 69:917-27. 2009

Collaborators

Detail Information

Publications23

  1. pmc Identification of thymus specific and developmentally regulated genes by an improved version of the mRNA differential display technique
    S Pascolo
    Interfakultäres Institut für Zellbiologie, Abt Immunologie Auf der Morgenstelle 15, Tubingen, Germany
    Dev Immunol 7:1-7. 1999
    ..Thus, we report an optimized, reproducible, and fast mRNA differential display method that overcomes the usual problems met with the originally described technique or its reported modifications...
  2. ncbi request reprint The non-classical HLA class I molecule HFE does not influence the NK-like activity contained in fresh human PBMCs and does not interact with NK cells
    Steve Pascolo
    Department of Immunology, Auf der Morgenstelle 15, 72076 Tubingen, Germany
    Int Immunol 17:117-22. 2005
    ..Moreover, using fluorescent HFE tetramers, we could confirm that blood NK cells as well as blood gammadelta T cells do not bind HFE. Altogether, our data indicate that HFE does not affect the NK activity contained in the PBMCs...
  3. ncbi request reprint HLA class I transgenic mice: development, utilisation and improvement
    Steve Pascolo
    Department of Immunology, University of Tubingen, Auf der Morgenstelle 15, 72076 Tubingen, Germany
    Expert Opin Biol Ther 5:919-38. 2005
    ..This review presents the similarities and differences between mouse and human MHC I antigen processing machinery, and describes the development and utilisation of improving mouse models of human cytotoxic T cell immunity...
  4. ncbi request reprint Plasmid DNA- and messenger RNA-based anti-cancer vaccination
    Benjamin Weide
    Department of Dermatology, University of Tubingen, Liebermeisterstrasse 25, Tubingen, Germany
    Immunol Lett 115:33-42. 2008
    ..Combining optimized MNAV formulations and parallel adjuvant treatments could allow to turn MNAV-based vaccines into efficient anti-tumor immunotherapies in humans...
  5. ncbi request reprint A MAGE-A1 HLA-A A*0201 epitope identified by mass spectrometry
    S Pascolo
    Institut for Cell Biology, Department Immunology, University of Tubingen, Auf der Morgenstelle 15, 72076 Tubingen, Germany
    Cancer Res 61:4072-7. 2001
    ....
  6. doi request reprint Results of the first phase I/II clinical vaccination trial with direct injection of mRNA
    Benjamin Weide
    Department of Dermatology, Institute for Cell Biology, University of Tubingen, Germany
    J Immunother 31:180-8. 2008
    ..However, a demonstration of clinical effectiveness of direct injection of copy mRNA for antitumor immunotherapy was not shown in this study and must be evaluated in subsequent trials...
  7. ncbi request reprint Spontaneous cellular uptake of exogenous messenger RNA in vivo is nucleic acid-specific, saturable and ion dependent
    J Probst
    Department of Immunology, Institute for Cell Biology, University of Tubingen, Tubingen, Germany
    Gene Ther 14:1175-80. 2007
    ..After injection into the human dermis, we could document the translation of the exogenous mRNA. Our results pave the way toward the use of mRNA as a vehicle for transient gene delivery in humans...
  8. ncbi request reprint Human peripheral blood mononuclear cells transfected with messenger RNA stimulate antigen-specific cytotoxic T-lymphocytes in vitro
    R Teufel
    CureVac GmbH, Paul Ehrlich Str 15, 72076, Tubingen, Germany
    Cell Mol Life Sci 62:1755-62. 2005
    ..Thus, mRNA-transfected PBMCs are a convenient replacement of mRNA-transfected Mo-DCs for the in vitro monitoring of natural or vaccine-induced immune responses...
  9. doi request reprint Direct injection of protamine-protected mRNA: results of a phase 1/2 vaccination trial in metastatic melanoma patients
    Benjamin Weide
    Department of Dermatology, Division of Dermatooncology, University of Tubingen, Tubingen, Germany
    J Immunother 32:498-507. 2009
    ....
  10. doi request reprint Novel multi-peptide vaccination in Hla-A2+ hormone sensitive patients with biochemical relapse of prostate cancer
    Susan Feyerabend
    Department of Urology, University of Tubingen, Germany
    Prostate 69:917-27. 2009
    ..A phase I/II trial was conducted to assess feasibility and tolerability of tumor associated antigen peptide vaccination in hormone sensitive prostate carcinoma (PC) patients with biochemical recurrence after primary surgical treatment...
  11. doi request reprint Mass spectrometric identification of an HLA-A*0201 epitope from Plasmodium falciparum MSP-1
    Jean Philippe Carralot
    Department of Immunology, Institute for Cell Biology, University of Tubingen, Auf der Morgenstelle 15, D 72076 Tubingen, Germany
    Int Immunol 20:1451-6. 2008
    ..falciparum. This epitope can be used as a tool for the immunomonitoring of natural or vaccine-induced CTL immune responses against malaria and could eventually be proposed as a component of an anti-malaria peptide-based vaccine...
  12. ncbi request reprint Expression of iron transport proteins divalent metal transporter-1, Ferroportin-1, HFE and transferrin receptor-1 in human monocyte-derived dendritic cells
    Martin Brinkmann
    Department of Immunology, Institute for Cell Biology, Tubingen, Germany
    Cell Biochem Funct 25:287-96. 2007
    ....
  13. ncbi request reprint Messenger RNA-based vaccines
    Steve Pascolo
    CureVac GmbH, Paul Ehrlich Strasse 15, 72076 Tubingen, Germany
    Expert Opin Biol Ther 4:1285-94. 2004
    ..The paper will present the official, industrial and financial aspects of mRNA-based vaccination that are paving the way for therapeutic and prophylactic drugs with mRNA as the active component...
  14. ncbi request reprint Toll-like receptor-dependent activation of several human blood cell types by protamine-condensed mRNA
    Birgit Scheel
    CureVac GmbH, Tubingen, Germany
    Eur J Immunol 35:1557-66. 2005
    ..The studies presented here explain the capacity of protamine-RNA to act as a vaccine, and pave the way towards the development of safe and efficient mRNA-based immunotherapies...
  15. ncbi request reprint Therapeutic anti-tumor immunity triggered by injections of immunostimulating single-stranded RNA
    Birgit Scheel
    Institute for Cell Biology, Department of Immunology, University of Tubingen, Tubingen, Germany
    Eur J Immunol 36:2807-16. 2006
    ..In conclusion, RNA molecules are potent and safe danger signals that are relevant for active immunotherapy strategies aimed at the eradication of solid tumors...
  16. ncbi request reprint CD8+ T cells specific for a potential HLA-A*0201 epitope from Chlamydophila pneumoniae are present in the PBMCs from infected patients
    Jean Philippe Carralot
    Department of Immunology, Institute for Cell Biology, University of Tubingen, Auf der Morgenstelle 15, 72076 Tubingen, Germany
    Int Immunol 17:591-7. 2005
    ..Our studies pave the way to the immunomonitoring of the anti-Cpn CTL immune response present in patients suffering from different diseases potentially linked to Cpn or anti-Cpn immunity...
  17. ncbi request reprint Immunostimulating capacities of stabilized RNA molecules
    Birgit Scheel
    CureVac GmbH, Tubingen, Germany
    Eur J Immunol 34:537-47. 2004
    ..We postulate that phosphorothioate RNA, which mimics trans-stabilized RNA, is a new PAMP...
  18. ncbi request reprint Co-transfection of messenger RNA and siRNA as a method to study the efficiency of siRNA
    Thorsten Mutzke
    CureVac GmbH, Tubingen, Germany
    Nucleosides Nucleotides Nucleic Acids 24:147-52. 2005
    ..This technique can be used as a fast method to test siRNA even when they target genes that cannot be stably expressed in the cells of interest...
  19. pmc Direct recognition by alphabeta cytolytic T cells of Hfe, a MHC class Ib molecule without antigen-presenting function
    Pierre S Rohrlich
    Unité d Immunité Cellulaire Antivirale, Departement d Immunologie, Institut Pasteur, 75724 Paris, France
    Proc Natl Acad Sci U S A 102:12855-60. 2005
    ....
  20. ncbi request reprint Rab27a regulates phagosomal pH and NADPH oxidase recruitment to dendritic cell phagosomes
    Carolina Jancic
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Nat Cell Biol 9:367-78. 2007
    ....
  21. pmc Identification of Chlamydia pneumoniae-derived mouse CD8 epitopes
    Anne Sarén
    Department of Vaccines, National Public Health Institute, Helsinki, Finland
    Infect Immun 70:3336-43. 2002
    ..Identification of these natural CD8 epitopes provides tools for characterization of CD8(+) T-cell function in vivo and generation of epitope-specific prevention strategies...
  22. ncbi request reprint Vaccination with messenger RNA
    Steve Pascolo
    CureVac GmbH, Tubingen, Germany
    Methods Mol Med 127:23-40. 2006
    ..This chapter describes the production of mRNA and the preparation of the two types of mRNA-based vaccines tested in humans...
  23. ncbi request reprint A conserved sequence in the mouse variable T cell receptor alpha recombination signal sequence 23-bp spacer can affect recombination
    Jochen Probst
    Institute for Cell Biology, Department of Immunology, Auf der Morgenstelle 15, D 72076 Tubingen, Germany
    Eur J Immunol 34:2179-90. 2004
    ..We hypothesize that these proteins can affect recombination at the TCR alpha delta locus...