Steffen Backert

Summary

Affiliation: Otto-von-Guericke University
Country: Germany

Publications

  1. doi request reprint Emerging roles of Abl family tyrosine kinases in microbial pathogenesis
    Steffen Backert
    Department of Medical Microbiology, Otto von Guericke University, D 39120 Magdeburg, Germany
    Trends Biochem Sci 33:80-90. 2008
  2. doi request reprint VirB2 and VirB5 proteins: specialized adhesins in bacterial type-IV secretion systems?
    Steffen Backert
    Otto von Guericke Universitat Magdeburg, Institut fur Medizinische Mikrobiologie, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Trends Microbiol 16:409-13. 2008
  3. doi request reprint Role of type IV secretion in Helicobacter pylori pathogenesis
    Steffen Backert
    Otto von Guericke Universitat Magdeburg, Institut fur Medizinische Mikrobiologie, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Cell Microbiol 10:1573-81. 2008
  4. doi request reprint Dual infection system identifies a crucial role for PKA-mediated serine phosphorylation of the EPEC-Tir-injected effector protein in regulating Rac1 function
    Sabine Brandt
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Str 44, D 39120 Magdeburg, Germany
    Cell Microbiol 11:1254-71. 2009
  5. ncbi request reprint Gene expression and protein profiling of AGS gastric epithelial cells upon infection with Helicobacter pylori
    Steffen Backert
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    Proteomics 5:3902-18. 2005
  6. ncbi request reprint Helicobacter exploits integrin for type IV secretion and kinase activation
    Terry Kwok
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Nature 449:862-6. 2007
  7. ncbi request reprint Activation of Abl by Helicobacter pylori: a novel kinase for CagA and crucial mediator of host cell scattering
    Ina Tammer
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    Gastroenterology 132:1309-19. 2007
  8. doi request reprint Helicobacter pylori activates protein kinase C delta to control Raf in MAP kinase signalling: role in AGS epithelial cell scattering and elongation
    Sabine Brandt
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    Cell Motil Cytoskeleton 66:874-92. 2009
  9. ncbi request reprint Use of a novel coinfection system reveals a role for Rac1, H-Ras, and CrkII phosphorylation in Helicobacter pylori-induced host cell actin cytoskeletal rearrangements
    Sabine Brandt
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    FEMS Immunol Med Microbiol 50:190-205. 2007
  10. pmc NF-kappaB activation and potentiation of proinflammatory responses by the Helicobacter pylori CagA protein
    Sabine Brandt
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Proc Natl Acad Sci U S A 102:9300-5. 2005

Collaborators

Detail Information

Publications43

  1. doi request reprint Emerging roles of Abl family tyrosine kinases in microbial pathogenesis
    Steffen Backert
    Department of Medical Microbiology, Otto von Guericke University, D 39120 Magdeburg, Germany
    Trends Biochem Sci 33:80-90. 2008
    ..Therapeutic intervention against Abl kinase activity might be an effective and novel strategy to combat serious microbial diseases...
  2. doi request reprint VirB2 and VirB5 proteins: specialized adhesins in bacterial type-IV secretion systems?
    Steffen Backert
    Otto von Guericke Universitat Magdeburg, Institut fur Medizinische Mikrobiologie, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Trends Microbiol 16:409-13. 2008
    ..Here, we discuss this important issue and recent progress in the field...
  3. doi request reprint Role of type IV secretion in Helicobacter pylori pathogenesis
    Steffen Backert
    Otto von Guericke Universitat Magdeburg, Institut fur Medizinische Mikrobiologie, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Cell Microbiol 10:1573-81. 2008
    ..The contribution of these signalling cascades to H. pylori pathogenesis is discussed...
  4. doi request reprint Dual infection system identifies a crucial role for PKA-mediated serine phosphorylation of the EPEC-Tir-injected effector protein in regulating Rac1 function
    Sabine Brandt
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Str 44, D 39120 Magdeburg, Germany
    Cell Microbiol 11:1254-71. 2009
    ..Thus, the co-infection approach is a powerful strategy for defining novel effector function with important implications for characterizing mechanisms and regulatory signalling pathways in bacterial pathogenesis...
  5. ncbi request reprint Gene expression and protein profiling of AGS gastric epithelial cells upon infection with Helicobacter pylori
    Steffen Backert
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    Proteomics 5:3902-18. 2005
    ..pylori virulence determinants. This study serves as a basis for future work on transcription and proteome analyses of the H. pylori infection model...
  6. ncbi request reprint Helicobacter exploits integrin for type IV secretion and kinase activation
    Terry Kwok
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Nature 449:862-6. 2007
    ..Our findings provide insights into the role of integrins in H.-pylori-induced pathogenesis. CagL may be exploited as a new molecular tool for our further understanding of integrin signalling...
  7. ncbi request reprint Activation of Abl by Helicobacter pylori: a novel kinase for CagA and crucial mediator of host cell scattering
    Ina Tammer
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    Gastroenterology 132:1309-19. 2007
    ..Src then is inactivated rapidly, suggesting the presence of another host tyrosine kinase to ensure constant CagA phosphorylation in sustained Hp infections. We aimed to identify this kinase...
  8. doi request reprint Helicobacter pylori activates protein kinase C delta to control Raf in MAP kinase signalling: role in AGS epithelial cell scattering and elongation
    Sabine Brandt
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    Cell Motil Cytoskeleton 66:874-92. 2009
    ..Finally, we show that PKC-delta specifically targets Raf kinase to stimulate the Erk1/2 kinase pathway, which is also crucial for phenotypical outcome. Thus, PKC-delta is another important mediator of H. pylori-induced pathogenesis...
  9. ncbi request reprint Use of a novel coinfection system reveals a role for Rac1, H-Ras, and CrkII phosphorylation in Helicobacter pylori-induced host cell actin cytoskeletal rearrangements
    Sabine Brandt
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    FEMS Immunol Med Microbiol 50:190-205. 2007
    ..In addition, we show that ADP-ribosylation of CrkII by ExoT blocks phosphorylation of CrkII at Y-221, which is also important for the CagA-induced signalling...
  10. pmc NF-kappaB activation and potentiation of proinflammatory responses by the Helicobacter pylori CagA protein
    Sabine Brandt
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Proc Natl Acad Sci U S A 102:9300-5. 2005
    ..Thus, CagA is a multifunctional protein capable of effecting both actin remodeling and potentiation of chemokine release...
  11. ncbi request reprint Type IV secretion systems and their effectors in bacterial pathogenesis
    Steffen Backert
    Otto von Guericke Universitat Magdeburg, Institut fur Medizinische Mikrobiologie, Leipziger Str 44, D 39120 Magdeburg, Germany
    Curr Opin Microbiol 9:207-17. 2006
    ....
  12. doi request reprint Serine phosphorylation of cortactin controls focal adhesion kinase activity and cell scattering induced by Helicobacter pylori
    Nicole Tegtmeyer
    Department of Microbiology, Otto von Guericke University, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Cell Host Microbe 9:520-31. 2011
    ..Using Hp as a model, this study unravels a previously unrecognized FAK activation pathway. We propose that Hp targets cortactin to protect the gastric epithelium from excessive cell lifting and ensure sustained infection in the stomach...
  13. pmc c-Src and c-Abl kinases control hierarchic phosphorylation and function of the CagA effector protein in Western and East Asian Helicobacter pylori strains
    Doreen Mueller
    University of Magdeburg, Department of Medical Microbiology, Magdeburg, Germany
    J Clin Invest 122:1553-66. 2012
    ..These results provide insight for clinical H. pylori typing and clarify the role of phosphorylated bacterial effector proteins in pathogenesis...
  14. doi request reprint Importance of EGF receptor, HER2/Neu and Erk1/2 kinase signalling for host cell elongation and scattering induced by the Helicobacter pylori CagA protein: antagonistic effects of the vacuolating cytotoxin VacA
    Nicole Tegtmeyer
    Department of Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    Cell Microbiol 11:488-505. 2009
    ..pylori to induce the latter phenotypes. These data demonstrate that VacA can downregulate CagA's effects on epithelial cells, a novel molecular mechanism showing how H. pylori can avoid excessive cellular damage...
  15. doi request reprint Role of Abl and Src family kinases in actin-cytoskeletal rearrangements induced by the Helicobacter pylori CagA protein
    Nicole Tegtmeyer
    University of Magdeburg, Department of Medical Microbiology, Leipziger Str 44, D 39120 Magdeburg, Germany
    Eur J Cell Biol 90:880-90. 2011
    ..Detailed investigation of these pathways will help to yield novel insights and to elucidate the mechanisms of H. pylori-induced pathogenesis...
  16. ncbi request reprint Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli
    Malgorzata Krause-Gruszczynska
    Department of Medical Microbiology, Otto von Guericke University Magdeburg, Magdeburg, Germany
    FEMS Microbiol Lett 274:9-16. 2007
    ..These results demonstrate that CadF is an important pathogenicity factor. The difference between CadF of C. jejuni and C. coli may potentially be exploited to discriminate these species in food and clinical specimens...
  17. pmc Induction of TLR-2 and TLR-5 expression by Helicobacter pylori switches cagPAI-dependent signalling leading to the secretion of IL-8 and TNF-α
    Suneesh Kumar Pachathundikandi
    Institute for Medical Microbiology, Otto von Guericke University, Magdeburg, Germany
    PLoS ONE 6:e19614. 2011
    ..pylori-induced expression of TLR-2 and TLR-5 can qualitatively shift cagPAI-dependent to cagPAI-independent pro-inflammatory signalling pathways with possible impact on the outcome of H. pylori-associated diseases...
  18. ncbi request reprint Conjugative plasmid DNA transfer in Helicobacter pylori mediated by chromosomally encoded relaxase and TraG-like proteins
    Steffen Backert
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Str 44, D 39120 Magdeburg, Germany
    Microbiology 151:3493-503. 2005
    ..pylori strains...
  19. ncbi request reprint Role of the small Rho GTPases Rac1 and Cdc42 in host cell invasion of Campylobacter jejuni
    Malgorzata Krause-Gruszczynska
    Department of Medical Microbiology, Otto von Guericke University Magdeburg, Leipziger Str 44, D 39120 Magdeburg, Germany
    Cell Microbiol 9:2431-44. 2007
    ..Collectively, our results suggest that C. jejuni invade host target cells by a unique mechanism and the activation of the Rho GTPase members Rac1 and Cdc42 plays a crucial role in this entry process...
  20. ncbi request reprint Subproteomes of soluble and structure-bound Helicobacter pylori proteins analyzed by two-dimensional gel electrophoresis and mass spectrometry
    Steffen Backert
    Otto von Guericke University, Dept of Medical Microbiology, Magdeburg, Germany
    Proteomics 5:1331-45. 2005
    ..pylori proteome. Such subproteomes in the public domain will be effective instruments for identifying new virulence factors and antigens of potential diagnostic and/or curative value against infections with this important pathogen...
  21. ncbi request reprint Tyrosine-phosphorylated bacterial effector proteins: the enemies within
    Steffen Backert
    Otto von Guericke Universitat Magdeburg, Institut fur Medizinische Mikrobiologie, Leipziger Str 44, D 39120 Magdeburg, Germany
    Trends Microbiol 13:476-84. 2005
    ..The discovery that different bacterial pathogens use this common strategy to subvert host-cell function suggests that more examples will soon emerge...
  22. ncbi request reprint Strain-specific expression profiles of virulence genes in Helicobacter pylori during infection of gastric epithelial cells and granulocytes
    Steffi Gieseler
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Street 44, 39120 Magdeburg, Germany
    Microbes Infect 7:437-47. 2005
    ..The use of Taqman-PCR to analyse the expression of mRNA of a bacterial pathogen in response to a changing host environment enabled us to identify variable and strain-specific transcription profiles in a sensitive and reproducible manner...
  23. doi request reprint Morphologic, genetic, and biochemical characterization of Helicobacter magdeburgensis, a novel species isolated from the intestine of laboratory mice
    Francisco Rivas Traverso
    Department of Medical Microbiology, Otto von Guericke University Magdeburg, D 39120 Magdeburg, Germany
    Helicobacter 15:403-15. 2010
    ..The aim of this study was to isolate and characterize a putative novel EHS that has previously been detected by PCR screening of specific-pathogen-free mice...
  24. pmc Functional analysis of the cag pathogenicity island in Helicobacter pylori isolates from patients with gastritis, peptic ulcer, and gastric cancer
    Steffen Backert
    Department of Medical Microbiology, Otto von Guericke University, D 39120 Magdeburg, Germany
    Infect Immun 72:1043-56. 2004
    ..pylori-induced responses with any particular patient group. This may indicate that the determination of disease development is highly complex and involves multiple bacterial and/or host factors...
  25. ncbi request reprint Interplay of bacterial toxins with host defence: molecular mechanisms of immunomodulatory signalling
    Steffen Backert
    Institute of Medical Microbiology, Otto von Guericke University Magdeburg, Leipziger Str 44, D 39120 Magdeburg, Germany
    Int J Med Microbiol 295:519-30. 2005
    ..Here, we review important aspects on the molecular mechanisms of immunomodulatory interference by bacterial toxins, with emphasis on the signalling induced in the intestinal epithelium...
  26. ncbi request reprint Helicobacter pylori HopH (OipA) and bacterial pathogenicity: genetic and functional genomic analysis of hopH gene polymorphisms
    Anar Dossumbekova
    Second Department of Internal Medicine and Gastroenterology, Technical University of Munich, Munich, Germany
    J Infect Dis 194:1346-55. 2006
    ..Expression of the Helicobacter pylori outer membrane protein HopH is regulated by phase variation within a CT dinucleotide repeat motif of the hopH gene...
  27. ncbi request reprint The Helicobacter pylori CagA protein induces tyrosine dephosphorylation of ezrin
    Matthias Selbach
    Max Planck Institut für Infektionsbiologie, Abt Molekulare Biologie, Berlin, Germany
    Proteomics 4:2961-8. 2004
    ..pylori pathogenesis and might also help to explain the development of gastric cancer...
  28. doi request reprint Pathogenesis of Helicobacter pylori infection
    Javier Torres
    Unidad de Investigacion en Enfermedades Infecciosas, UMAE Pediatria, IMSS, Mexico
    Helicobacter 13:13-7. 2008
    ..An animal model using the Mongolian gerbil is a useful system to study the gastric pathology of H. pylori infection...
  29. ncbi request reprint Helicobacter pylori type IV secretion, host cell signalling and vaccine development
    Steffen Backert
    Max Planck Institut für Infektionsbiologie, Abt Molekulare Biologie, Berlin, Germany
    Keio J Med 51:6-14. 2002
    ....
  30. pmc cag+ Helicobacter pylori induces homotypic aggregation of macrophage-like cells by up-regulation and recruitment of intracellular adhesion molecule 1 to the cell surface
    Stefan Moese
    Abteilung Molekulare Biologie, Max Planck Institut für Infektionsbiologie, D 10117 Berlin, Germany
    Infect Immun 70:4687-91. 2002
    ..This signaling may regulate cell-cell interactions and inflammatory responses...
  31. pmc The Helicobacter pylori CagA protein induces cortactin dephosphorylation and actin rearrangement by c-Src inactivation
    Matthias Selbach
    Max Planck Institut für Infektionsbiologie, Abt Molekulare Biologie, Schumannstrasse 20 21, D 10117 Berlin, Germany
    EMBO J 22:515-28. 2003
    ..This is the first report of a bacterial virulence factor that inhibits signalling of a eukaryotic tyrosine kinase and on a role of c-Src inactivation in host cell cytoskeletal rearrangements...
  32. pmc Functional analysis of the Helicobacter pylori cag pathogenicity island reveals both VirD4-CagA-dependent and VirD4-CagA-independent mechanisms
    Matthias Selbach
    Abteilung Molekulare Biologie, Max Planck Institut für Infektionsbiologie, D 10117 Berlin, Germany
    Infect Immun 70:665-71. 2002
    ..The function of VirD4 as a possible adapter protein which guides CagA into the type IV secretion channel is presented in a model...
  33. pmc Helicobacter pylori induces AGS cell motility and elongation via independent signaling pathways
    Stefan Moese
    Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany
    Infect Immun 72:3646-9. 2004
    ..The cag pathogenicity island appears to be nonessential for induction of motility, whereas the elongation phenotype depends on translocation and phosphorylation of CagA...
  34. ncbi request reprint The Helicobacter pylori CagA protein disrupts matrix adhesion of gastric epithelial cells by dephosphorylation of vinculin
    Stefan Moese
    Department of Molecular Biology, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany
    Cell Microbiol 9:1148-61. 2007
    ..We hypothesize that CagA-mediated inactivation of vinculin is a key step in the mechanism by which H. pylori induces damage to the gastric epithelium and represents an important step in disease development...
  35. ncbi request reprint Src is the kinase of the Helicobacter pylori CagA protein in vitro and in vivo
    Matthias Selbach
    Max Planck Institut für Infektionsbiologie, Abt Molekulare Biologie, Schumannstrasse 20 21, D 10117 Berlin, Germany
    J Biol Chem 277:6775-8. 2002
    ....
  36. ncbi request reprint Cortactin: an Achilles' heel of the actin cytoskeleton targeted by pathogens
    Matthias Selbach
    University of Southern Denmark, Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, Denmark
    Trends Microbiol 13:181-9. 2005
    ..This has an important impact on how pathogens abuse cortactin to modulate the architecture of the host actin cytoskeleton...
  37. pmc Helicobacter pylori induces transendothelial migration of activated memory T cells
    Karin Enarsson
    Department of Medical Microbiology and Immunology, Goteborg University, Box 435, 405 30 Goteborg, Sweden
    Infect Immun 73:761-9. 2005
    ..In conclusion, our data indicate that live H. pylori and its secreted products contribute to T-cell recruitment to the gastric mucosa and that the responding T cells have an activated memory Th1 phenotype...
  38. pmc Specific entry of Helicobacter pylori into cultured gastric epithelial cells via a zipper-like mechanism
    Terry Kwok
    Abteilung Infektionsbiologie, Max Planck Institut fur Biologie, D 72076 Tubingen, Germany
    Infect Immun 70:2108-20. 2002
    ..pylori into AGS cells. Collectively, these results demonstrate that the entry of H. pylori into AGS cells occurs via a zipper-like mechanism which involves various host signal transduction events...
  39. pmc R-loop-dependent rolling-circle replication and a new model for DNA concatemer resolution by mitochondrial plasmid mp1
    Steffen Backert
    Department of Botany and Microbiology, Auburn University, Auburn, AL 36849, USA
    EMBO J 21:3128-36. 2002
    ..mp1 is a unique eukaryotic plasmid that expresses features of phages like T4 and could serve as a model system for replication and maintenance of DNA concatemers...
  40. ncbi request reprint DNA binding specificity of the replication initiator protein, DnaA from Helicobacter pylori
    Anna Zawilak
    Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland
    J Mol Biol 334:933-47. 2003
    ..pylori DnaA specificity. Of these, the most interesting is probably the triad PTL; all three residues show significant divergence from the consensus, and Thr398 is the most divergent residue of all...
  41. ncbi request reprint Helicobacter pylori induce neutrophil transendothelial migration: role of the bacterial HP-NAP
    Mikael Brisslert
    Department of Medical Microbiology and Immunology, Goteborg University, Sweden
    FEMS Microbiol Lett 249:95-103. 2005
    ..pylori induced neutrophil transendothelial migration. We propose that HP-NAP contributes to the continuous recruitment of neutrophils to the gastric mucosa of H. pylori infected individuals...
  42. pmc Conjugative transfer of chromosomally encoded antibiotic resistance from Helicobacter pylori to Campylobacter jejuni
    Omar A Oyarzabal
    Department of Poultry Science, Auburn University, Auburn, Alabama 36849, USA
    J Clin Microbiol 45:402-8. 2007
    ..The significance of this DNA uptake by C. jejuni in the context of acquiring antibiotic resistance is discussed...
  43. doi request reprint Molecular mechanisms of epithelial-barrier disruption by Helicobacter pylori
    Silja Wessler
    Junior Research Group, Paul Ehrlich Institute, D 63225 Langen, Germany
    Trends Microbiol 16:397-405. 2008
    ..We propose a stepwise model for how H. pylori interacts with components of focal adhesions and intercellular tight and adherens junctions to disrupt the epithelial layer, providing novel insights into the pathogenesis of H. pylori...