Research Topics
| Vasilis NtziachristosSummaryCountry: Germany Publications
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Detail Information
Publications
Going deeper than microscopy: the optical imaging frontier in biologyVasilis Ntziachristos
Institute for Biological and Medical Imaging, Helmholtz Zentrum München und Technische Universität München, Munich, Germany
Nat Methods 7:603-14. 2010..Key characteristics associated with different imaging implementations are described and the potential of these technologies in biological applications is discussed...- Optoacoustic imaging for clinical applications: devices and methodsAlexander Dima
Institute for Biological and Medical Imaging, Chair for Biological Imaging, Technische Univeristät München and Helmholtz Zentrum München, Munich, Germany
Expert Opin Med Diagn 5:263-72. 2011..These up-and-coming features offer a wider variety of optoacoustic approaches that are likely to be clinically deployed in the near future... - Optical and opto-acoustic imagingVasilis Ntziachristos
Chair for Biological Imaging, Institute for Biological and Medical Imaging Technische Universität München and Helmholtz Zentrum München, Munich, Germany
Recent Results Cancer Res 187:133-50. 2013..These new-found imaging abilities directly relate to preclinical screening applications in animal models and are foreseen to significantly impact clinical decision making as well... 
Opto-acoustic imaging of drug discovery biomarkersBohumil Bednar
Department of Imaging, Merck Research Laboratories, 770 Sumneytown Pike, West Point, PA 19486 0004, USA
Curr Pharm Biotechnol 13:2117-27. 2012....
Multifunctional Nanocarriers for diagnostics, drug delivery and targeted treatment across blood-brain barrier: perspectives on tracking and neuroimagingSonu Bhaskar
Comprehensive Pneumology Centre, Institute of Lung Biology and Disease, Helmholtz Zentrum Munchen, Neuherberg, Germany
Part Fibre Toxicol 7:3. 2010....- Clinical translation of optical and optoacoustic imagingVasilis Ntziachristos
Biological Imaging and Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Munich, Germany
Philos Trans A Math Phys Eng Sci 369:4666-78. 2011..This performance is showcased herein and examples are given to illustrate how it is possible to shift the paradigm of optical clinical translation... - Current concepts and future perspectives on surgical optical imaging in cancerVasilis Ntziachristos
Institute for Biological and Medical Imaging, Munich, Germany
J Biomed Opt 15:066024. 2010..Key future directions that have the potential to shift the paradigm of surgical health care are also discussed... - Optical imaging of molecular signatures in pulmonary inflammationVasilis Ntziachristos
Institute for Biological and Medical Imaging, Helmholtz Zentrum München and Technische Universität München, Munich, Germany
Proc Am Thorac Soc 6:416-8. 2009..Overall, the proposed imaging method can be used with a significantly wider range of possible targets and applications in lung imaging... - Optical imaging of cancer heterogeneity with multispectral optoacoustic tomographyEva Herzog
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Neuherberg, Germany
Radiology 263:461-8. 2012..To investigate whether multispectral optoacoustic tomography (MSOT) can reveal the heterogeneous distributions of exogenous agents of interest and vascular characteristics through tumors of several millimeters in diameter in vivo... - Fast automatic segmentation of anatomical structures in x-ray computed tomography images to improve fluorescence molecular tomography reconstructionMarcus Freyer
Technical University Munich, Biological Imaging, Munich, Germany
J Biomed Opt 15:036006. 2010..The algorithm and its utilization in an inverse FMT scheme that uses priors is demonstrated on mouse images... - Accurate model-based reconstruction algorithm for three-dimensional optoacoustic tomographyX Luís Dean-Ben
Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, Neuherberg, Germany
IEEE Trans Med Imaging 31:1922-8. 2012..Superior performance versus commonly-used backprojection inversion algorithms is showcased by numerical simulations and phantom experiments... - Model-based optoacoustic inversions with incomplete projection dataAndreas Buehler
Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Ingoldstädter Landstrafsse 1, D 85764 Neuherberg, Germany
Med Phys 38:1694-704. 2011..In many tomographic imaging scenarios, however, only limited-view or partial projection data are available, which has been shown to generate image artifacts and overall loss of quantification accuracy... - Acceleration of optoacoustic model-based reconstruction using angular image discretizationX Luís Dean-Ben
Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
IEEE Trans Med Imaging 31:1154-62. 2012..It is further anticipated that the new approach will greatly facilitate development of high performance 3-D optoacoustic reconstruction methods... - Fast multispectral optoacoustic tomography (MSOT) for dynamic imaging of pharmacokinetics and biodistribution in multiple organsAdrian Taruttis
Institute for Biological and Medical Imaging, Helmholtz Zentrum Munich, Technical University Munich, Munich, Germany
PLoS ONE 7:e30491. 2012..Overall, such imaging performance introduces previously undocumented capabilities of fast, high resolution in vivo imaging of the fate of optical agents for drug discovery and basic biological research... - Fast semi-analytical model-based acoustic inversion for quantitative optoacoustic tomographyAmir Rosenthal
Institute for Biological and Medical Imaging, and Chair for Biological Imaging, Technische Universität of München and Helmholtz Zentrum München, 85764 Neuherberg, Germany
IEEE Trans Med Imaging 29:1275-85. 2010.... - Multispectral opto-acoustic tomography (MSOT) of the brain and glioblastoma characterizationNeal C Burton
Institute for Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany
Neuroimage 65:522-8. 2013..We also demonstrate how MSOT offers several compelling features for brain research and allows time-dependent detection and quantification of brain parameters that are not available using other imaging methods without invasive procedures... - Translational optical imagingAdrian Taruttis
Institute for Biological and Medical Imaging, Helmholtz Zentrum Munchen, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany
AJR Am J Roentgenol 199:263-71. 2012.... - Real-time intraoperative fluorescence imaging system using light-absorption correctionGeorge Themelis
Technische Universitat Munchen, Institute for Biological and Medical Imaging, Arcisstrasse 21, 80333 München, Germany
J Biomed Opt 14:064012. 2009.... - Imaging performance of a hybrid x-ray computed tomography-fluorescence molecular tomography system using priorsAngelique Ale
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Ingolstadter Landstrasse 1, D 85764 Neuherberg, Germany
Med Phys 37:1976-86. 2010..The unique data set studied attains accurately registered data of high spatially sampled photon fields propagating through tissue along 360 degrees projections... - Multispectral optoacoustic tomography by means of normalized spectral ratioThomas Jetzfellner
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Ingolstaedter Landstr 1, 85764 Neuherberg, Germany
Opt Lett 36:4176-8. 2011..Herein we introduce a spectral ratio approach that accounts for photon fluence variations. The performance and imaging improvement achieved with the proposed method is showcased both numerically and experimentally in phantoms and mice... - Enhancing surgical vision by using real-time imaging of αvβ3-integrin targeted near-infrared fluorescent agentGeorge Themelis
Institute for Biological and Medical Imaging IBMI, Technische Universität München and Helmholtz Zentrum München, Ingolstaedter Landstrasse 1, Neuherberg, Germany
Ann Surg Oncol 18:3506-13. 2011..Via the use of targeted probes it also becomes highly tumor-specific and can lead to significant improvements in surgical procedures and outcome... - Blind source unmixing in multi-spectral optoacoustic tomographyJürgen Glatz
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Munich, Germany
Opt Express 19:3175-84. 2011..We further demonstrate its capacity with temporal unmixing on real-time MSOT data obtained in-vivo for enhancing the visualization of absorber agent flow in the mouse vascular system... - Polarization-sensitive optoacoustic tomography of optically diffuse tissuesDaniel Razansky
Institute for Biological and Medical Imaging, Helmholtz Zentrum Munchen, Neuherberg, Germany
Opt Lett 33:2308-10. 2008..The technique is expected to enable highly accurate imaging of polarization contrast in tissues, far beyond the current capabilities of pure optical polarization-imaging approaches... - Near-field radiofrequency thermoacoustic tomography with impulse excitationDaniel Razansky
Institute for Biological and Medical Imaging IBMI, Technische Universität München and Helmholtz Zentrum München, Ingolstaedter Landstr 1, D 85764 Neuherberg, Germany
Med Phys 37:4602-7. 2010..However, existing implementations are based on modulated-carrier-frequency amplification solutions, which are generally costly and cannot achieve ultrahigh-peak-power requirements essential for optimal thermoacoustic signal generation... - Efficient framework for model-based tomographic image reconstruction using wavelet packetsAmir Rosenthal
Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, Neuherberg, Germany
IEEE Trans Med Imaging 31:1346-57. 2012..The performance of the new methodology is demonstrated for the case of 2-D optoacoustic tomography for both numerically generated and experimental data... - Near-field thermoacoustic imaging with transmission line pulsersMurad Omar
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Ingolstädter Landstr 1, Neuherberg, Germany
Med Phys 39:4460-6. 2012..This work aimed at investigating transmission lines as a method to generate excitation pulses to improve the NRT resolution over previous implementations without compromising the energy coupled into tissue... - Motion clustering for deblurring multispectral optoacoustic tomography images of the mouse heartAdrian Taruttis
Helmholtz Zentrum München and Technische Universtität München, Institute for Biological and Medical Imaging, 85764 Neuherberg, Germany
J Biomed Opt 17:016009. 2012..The correction method presented could be generally applied to other MSOT imaging applications prone to motion artifacts, for example, by respiration and heartbeat... - Multispectral optoacoustic tomography of matrix metalloproteinase activity in vulnerable human carotid plaquesDaniel Razansky
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Munich, Germany
Mol Imaging Biol 14:277-85. 2012..This study is aimed at demonstrating a new method with high potential for noninvasive clinical cardiovascular diagnostics of vulnerable plaques using high-resolution deep-tissue multispectral optoacoustic tomography (MSOT) technology... - Model-based optoacoustic inversion with arbitrary-shape detectorsAmir Rosenthal
Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, 85764 Neuherberg, Germany
Med Phys 38:4285-95. 2011..This effect may lead to attenuation or distortion in the recorded signal and, consequently, in the reconstructed image... - Multispectral optoacoustic tomography (MSOT) scanner for whole-body small animal imagingRui Ma
Institute for Biological and Medical Imaging, Technical University of Munich, Neuherberg, Germany
Opt Express 17:21414-26. 2009..The system performance is determined in phantom experiments involving molecular probes and validated by imaging of small animals of various scales... - Volumetric real-time multispectral optoacoustic tomography of biomarkersDaniel Razansky
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Neuherberg, Germany
Nat Protoc 6:1121-9. 2011..Overall, the entire protocol can be completed within 15-30 min for acquisition of a whole-body multispectral data set from a living mouse... - Reconstruction of fluorescence distribution hidden in biological tissue using mesoscopic epifluorescence tomographySaskia Björn
Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
J Biomed Opt 16:046005. 2011..Furthermore, we present ex vivo data that show high spatial resolution and quantitative accuracy in heterogeneous tissues using GFP-like fluorescence in B6-albino mice up to a depth of 1100 μm... - Statistical approach for optoacoustic image reconstruction in the presence of strong acoustic heterogeneitiesX Luís Dean-Ben
Institute for Biological and Medical Imaging, Technical University of Munich, Neuherberg, Germany
IEEE Trans Med Imaging 30:401-8. 2011..This performance is directly related to in vivo small animal imaging applications involving imaging in the presence of bones, lungs, and other highly mismatched organs... - The effects of acoustic attenuation in optoacoustic signalsX Luís Dean-Ben
Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany
Phys Med Biol 56:6129-48. 2011..The validity and benefits of the suggested modelling and correction approaches are experimentally validated in phantom measurements... - Artefact reduction in optoacoustic tomographic imaging by estimating the distribution of acoustic scatterersX Luís Dean-Ben
Technical University of Munich and Helmholtz Center Munich, Institute for Biological and Medical Imaging, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
J Biomed Opt 17:110504. 2012..Experimental results in a tissue-mimicking phantom clearly demonstrate improved performance as compared to the case in which no information on the distribution of acoustic scatterers is available... - FMT-XCT: in vivo animal studies with hybrid fluorescence molecular tomography-X-ray computed tomographyAngelique Ale
Technische Universität München and Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Neuherberg, Germany
Nat Methods 9:615-20. 2012..These findings indicate that addition of FMT optics into the XCT gantry may be a potent upgrade for small-animal XCT systems... - Deep tissue optical and optoacoustic molecular imaging technologies for pre-clinical research and drug discoveryDaniel Razansky
Institute for Biological and Medical Imaging IBMI, Technische Universität München and Helmholtz Zentrum München, Ingolstädter Landstraβe 1, 85764 Neuherberg, Germany
Curr Pharm Biotechnol 13:504-22. 2012.... - Revisiting the normalized Born approximation: effects of scatteringThomas Pyka
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Neuherberg, Germany
Opt Lett 36:4329-31. 2011.... - In vivo imaging of CT26 mouse tumours by using cmHsp70.1 monoclonal antibodyStefan Stangl
Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universitat Munchen, and Clinical Cooperation Group CCG Innate Immunity in Tumor Biology, Helmholtz Zentrum Munchen, German Research Center for Environmental Health HMGU, Munich, Germany
J Cell Mol Med 15:874-87. 2011..The anti-tumoral activity of the cmHsp70.1 mAb is further supported by its capacity to mediate antibody-dependent cytotoxicity... - Near-field thermoacoustic tomography of small animalsStephan Kellnberger
Institute for Biological and Medical Imaging IBMI, Technische Universität München and Helmholtz Zentrum München, Ingolstaedter Landstrasse 1, D 85764 Neuherberg, Germany
Phys Med Biol 56:3433-44. 2011..The best spatial resolution achieved for those experiments was 150 µm... - Video rate optoacoustic tomography of mouse kidney perfusionAndreas Buehler
Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Ingoldstädter Landstrasse 1, 85764 Neuherberg, Germany
Opt Lett 35:2475-7. 2010..The previously undocumented capacity of whole-body optoacoustic tomography at a video rate has been demonstrated by visualizing mouse kidney perfusion using Indocyanine Green in vivo... - In vivo frequency domain optoacoustic tomographyStephan Kellnberger
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Neuherberg, Germany
Opt Lett 37:3423-5. 2012..The method developed demonstrated the ability to produce tomographic images of optical absorbing phantoms and in vivo images, by enabling visualization of the mouse tail following ICG injection... - Imaging the bio-distribution of fluorescent probes using multispectral epi-illumination cryoslicing imagingAthanasios Sarantopoulos
Chair for Biological Imaging, Technische Universitat Munchen, Munich, Germany
Mol Imaging Biol 13:874-85. 2011..However, the validation of probe bio-distribution during their development or the study of different disease models, in support of in vivo imaging studies, is not straightforward... - Non-invasive whole-body imaging of adult zebrafish with optoacoustic tomographyRui Ma
Technical University of Munich, Ingolstädter Landstraße 1, Neuherberg, Germany
Phys Med Biol 57:7227-37. 2012.... - Quantitative optoacoustic signal extraction using sparse signal representationAmir Rosenthal
Institute for Biological and Medical Imaging, Helmholtz Center Munich and Technical University of Munich, Neuherberg, D 85764, Germany
IEEE Trans Med Imaging 28:1997-2006. 2009.... - High resolution tumor targeting in living mice by means of multispectral optoacoustic tomographyAndreas Buehler
Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Ingoldstädter Landstraße 1, Neuherberg 85764, Germany
EJNMMI Res 2:14. 2012..abstract:.. - Near-infrared fluorescence catheter system for two-dimensional intravascular imaging in vivoR Nika Razansky
Biological Imaging, Technische Universität München and Institute for Biological and Medical Imaging, IBMI, Helmholtz Zentrum Munchen, Munich, Germany
Opt Express 18:11372-81. 2010..This imaging approach is geared toward in vivo molecular imaging of atherosclerotic biomarkers and is engineered to allow seamless integration into the cardiac catheterization laboratory... - Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomographyAdrian Taruttis
Institute for Biological and Medical Imaging, Technical University of Munich, Neuherberg, Germany
Opt Express 18:19592-602. 2010..We discuss the potential of this imaging ability in cardiovascular disease (CVD) research and clinical applications... - Ex-vivo assessment and non-invasive in vivo imaging of internal hemorrhages in Aga2/+ mutant miceVladimir Ermolayev
Institute for Biological and Medical Imaging, Helmholtz Zentrum Munchen, Building 56, Ingolstädter Landstraße 1, D 85764 Neuherberg, Germany
Biochem Biophys Res Commun 432:389-93. 2013..The disease phenotype was characterized on Aga2/+ vs. wild type mice between 6 and 9 days postnatal. Anatomical and functional findings obtained in-vivo were contrasted to the ex-vivo appearance of the same tissues under cryo-slicing... - Improving limited-projection-angle fluorescence molecular tomography using a co-registered x-ray computed tomography scanKarin Radrich
Institute for Biological and Medical Imaging, Helmholtz Zentrum Munchen, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany
J Biomed Opt 17:126011. 2012..This work relates to currently disseminated FMT systems, using limited projection scans, and can be employed to enhance their performance... - Sensitivity of molecular target detection by multispectral optoacoustic tomography (MSOT)Daniel Razansky
Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, Ingolstadter Landstrasse 1, D 85764 Neuherberg, Germany
Med Phys 36:939-45. 2009..The theoretical predictions are further validated in phantom experiments involving Cy5.5, a common near-infrared fluorescent molecular agent... - Hybrid system for simultaneous fluorescence and x-ray computed tomographyRalf B Schulz
Chair for Biological Imaging, Technical University Munich, 80333 Munich, Germany
IEEE Trans Med Imaging 29:465-73. 2010..The functional system characteristics are showcased, and images from a brain imaging study are shown, which are reconstructed using XCT-derived priors into the optical forward problem... - Optical and opto-acoustic interventional imagingAthanasios Sarantopoulos
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Munich, Germany
Ann Biomed Eng 40:346-66. 2012..Herein, we outline developments in emerging fluorescence and opto-acoustic sensing and imaging techniques that can lead to practical implementations toward improving interventional vision... - Fast scanning coaxial optoacoustic microscopyRui Ma
Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
Biomed Opt Express 3:1724-31. 2012..System performance is showcased in phantoms and in vivo imaging of microvasculature, achieving real time operation in two dimensions and penetration of 6 mm into optically dense human tissues... - Non-invasive carotid imaging using optoacoustic tomographyAlexander Dima
Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Ingoldstädter Landstrasse 1, 85764 Neuherberg, Germany
Opt Express 20:25044-57. 2012..Instead, feasibility to detect human carotids and carotid-sized vessels in clinically-relevant depths is better demonstrated with curved arrays and tomographic approaches... - Imaging of mesoscopic-scale organisms using selective-plane optoacoustic tomographyDaniel Razansky
Institute for Biological and Medical Imaging, Helmholtz Zentrum Munchen, Ingolstadter Landstrasse 1, D 85764 Neuherberg, Germany
Phys Med Biol 54:2769-77. 2009..The utility of the method is demonstrated on several whole-body models and small-animal extremities...