E A Nigg

Summary

Country: Germany

Publications

  1. pmc The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 158:901-14. 2002
  2. ncbi Centrosome aberrations: cause or consequence of cancer progression?
    Erich A Nigg
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopfersitz 18a, D 82152 Martinsried, Germany
    Nat Rev Cancer 2:815-25. 2002
  3. pmc Tousled-like kinase functions with the chromatin assembly pathway regulating nuclear divisions
    Pilar Carrera
    Abteilung Molekulare Entwicklungsbiologie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg, D 37077 Gottingen, Germany
    Genes Dev 17:2578-90. 2003
  4. ncbi Aurora kinases link chromosome segregation and cell division to cancer susceptibility
    Patrick Meraldi
    Massachusetts Institute of Technology, Dept of Biology, 77 Massachusetts Ave, Cambridge, MA 02139, USA
    Curr Opin Genet Dev 14:29-36. 2004
  5. pmc Human TPX2 is required for targeting Aurora-A kinase to the spindle
    Thomas A Kufer
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Cell Biol 158:617-23. 2002
  6. pmc Cep164, a novel centriole appendage protein required for primary cilium formation
    Susanne Graser
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried D 82152, Germany
    J Cell Biol 179:321-30. 2007
  7. pmc Rootletin forms centriole-associated filaments and functions in centrosome cohesion
    Susanne Bahe
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 171:27-33. 2005
  8. pmc Plk1 regulates mitotic Aurora A function through betaTrCP-dependent degradation of hBora
    Eunice H Y Chan
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Chromosoma 117:457-69. 2008
  9. pmc Persistence of DNA threads in human anaphase cells suggests late completion of sister chromatid decatenation
    Lily Hui Ching Wang
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Chromosoma 117:123-35. 2008
  10. ncbi Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001

Collaborators

Detail Information

Publications69

  1. pmc The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 158:901-14. 2002
    ..These findings identify the CDC14 phosphatase as an important regulator of central spindle formation and cytokinesis in a metazoan organism...
  2. ncbi Centrosome aberrations: cause or consequence of cancer progression?
    Erich A Nigg
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopfersitz 18a, D 82152 Martinsried, Germany
    Nat Rev Cancer 2:815-25. 2002
  3. pmc Tousled-like kinase functions with the chromatin assembly pathway regulating nuclear divisions
    Pilar Carrera
    Abteilung Molekulare Entwicklungsbiologie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg, D 37077 Gottingen, Germany
    Genes Dev 17:2578-90. 2003
    ..Overexpression of TLK alters the chromatin structure, suggesting that TLK mediates the activity of chromatin proteins. These results suggest that TLK coordinates cell cycle progression through the regulation of chromatin dynamics...
  4. ncbi Aurora kinases link chromosome segregation and cell division to cancer susceptibility
    Patrick Meraldi
    Massachusetts Institute of Technology, Dept of Biology, 77 Massachusetts Ave, Cambridge, MA 02139, USA
    Curr Opin Genet Dev 14:29-36. 2004
    ..Aurora kinases are frequently overexpressed in cancers and the identification of Aurora A as a cancer-susceptibility gene provides a strong link between mitotic errors and carcinogenesis...
  5. pmc Human TPX2 is required for targeting Aurora-A kinase to the spindle
    Thomas A Kufer
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Cell Biol 158:617-23. 2002
    ..We propose that human TPX2 is required for targeting Aurora-A kinase to the spindle apparatus. In turn, Aurora-A might regulate the function of TPX2 during spindle assembly...
  6. pmc Cep164, a novel centriole appendage protein required for primary cilium formation
    Susanne Graser
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried D 82152, Germany
    J Cell Biol 179:321-30. 2007
    ..Moreover, the localizations of Cep164 and ninein/Cep170 were mutually independent during interphase. These data implicate distal appendages in PC formation and identify Cep164 as an excellent marker for these structures...
  7. pmc Rootletin forms centriole-associated filaments and functions in centrosome cohesion
    Susanne Bahe
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 171:27-33. 2005
    ..The ability of rootletin to form centriole-associated fibers suggests a dynamic model for centrosome cohesion based on entangling filaments rather than continuous polymeric linkers...
  8. pmc Plk1 regulates mitotic Aurora A function through betaTrCP-dependent degradation of hBora
    Eunice H Y Chan
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Chromosoma 117:457-69. 2008
    ..This suggests that Plk1 controls Aurora A localization and function by regulating cellular levels of hBora...
  9. pmc Persistence of DNA threads in human anaphase cells suggests late completion of sister chromatid decatenation
    Lily Hui Ching Wang
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Chromosoma 117:123-35. 2008
    ..Moreover, they show that topoisomerase activity is required during anaphase for the resolution of PICH-positive threads, implying that the complete separation of sister chromatids occurs later than previously assumed...
  10. ncbi Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001
    ..Here, I give an overview of the many mitotic kinases that regulate cell division and the fidelity of chromosome transmission...
  11. ncbi Centrosome duplication: of rules and licenses
    Erich A Nigg
    Max Planck Institute for Biochemistry, Am Klopferspitz 18, Martinsried, Germany
    Trends Cell Biol 17:215-21. 2007
    ..Finally, Plk4 (also called Sak), a member of the Polo kinase family, has been identified as a novel positive regulator of centriole formation...
  12. ncbi Centrosome cohesion is regulated by a balance of kinase and phosphatase activities
    P Meraldi
    Department of Cell Biology, Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany
    J Cell Sci 114:3749-57. 2001
    ..These studies highlight the importance of protein phosphorylation in the control of centrosome cohesion, and they point to Nek2 and PP1 alpha as critical regulators of centrosome structure...
  13. ncbi Cloning and characterization of Plx2 and Plx3, two additional Polo-like kinases from Xenopus laevis
    P I Duncan
    Department of Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18a, Martinsried, D 82152, Germany
    Exp Cell Res 270:78-87. 2001
    ..However, the strikingly restricted pattern of expression of Plx2 and Plx3 observed in embryos strongly suggests that individual Plk family members perform at least partly distinct functions at later stages of development...
  14. ncbi Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases
    H H Silljé
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Curr Biol 11:1068-73. 2001
    ..These data indicate that Tlk family members regulate chromatin assembly during DNA replication, and they suggest a plausible explanation for the pleiotropic developmental defects of plant tousled mutants...
  15. ncbi The centrosome cycle
    P Meraldi
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    FEBS Lett 521:9-13. 2002
    ..Here we summarize recent information on the regulation of the centrosome cycle and its coordination with the chromosomal cell cycle...
  16. ncbi HURP is a Ran-importin beta-regulated protein that stabilizes kinetochore microtubules in the vicinity of chromosomes
    Herman H W Sillje
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Curr Biol 16:731-42. 2006
    ..How RanGTP regulates kinetochore-microtubule (K-fiber) formation is not presently understood...
  17. ncbi M-phase MELK activity is regulated by MPF and MAPK
    Caroline Badouel
    CNRS UMR 6061 Génétique et Développement, Universite de Rennes, Rennes, France
    Cell Cycle 5:883-9. 2006
    ..In addition, phosphorylation by MPF and MAPK enhances MELK activity in vitro. Taken together our results indicate that MELK phosphorylation by MPF and MAPK enhance its activity during M-phase...
  18. pmc Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP
    Ulf R Klein
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Mol Biol Cell 17:2547-58. 2006
    ..Our findings thus establish a functional module within the CPC that assembles on the N terminus of INCENP and controls centromere recruitment...
  19. pmc KIF14 and citron kinase act together to promote efficient cytokinesis
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Biol 172:363-72. 2006
    ..Atzori, E. Turco, R. Triolo, G.P. Dotto, et al. 2000. Neuron. 28:115-127), we find a general requirement for citron kinase in human cell division. Together, these findings identify a novel pathway required for efficient cytokinesis...
  20. ncbi Complex formation of Plk1 and INCENP required for metaphase-anaphase transition
    Hidemasa Goto
    Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
    Nat Cell Biol 8:180-7. 2006
    ..We propose that INCENP phosphorylation by Cdk1 is necessary for the recruitment of Plk1 to the kinetochore, and that the complex formation of Plk1 and Aurora-B on INCENP may play crucial roles in the regulation of chromosomal dynamics...
  21. ncbi The Polo kinase Plk4 functions in centriole duplication
    Robert Habedanck
    Department of Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Cell Biol 7:1140-6. 2005
    ..These findings provide an attractive explanation for the crucial function of Plk4 in cell proliferation and have implications for the role of Polo kinases in tumorigenesis...
  22. ncbi Purification of mitotic spindles from cultured human cells
    Herman H W Sillje
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Methods 38:25-8. 2006
    ..Sauer, R. Korner, A. Hanisch, A. Ries, E.A. Nigg, H.H.W. Sillje, Mol. Cell. Proteomics 4 (2005) 35-43]...
  23. pmc Different Plk1 functions show distinct dependencies on Polo-Box domain-mediated targeting
    Anja Hanisch
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Mol Biol Cell 17:448-59. 2006
    ..This opens the possibility that PBD-directed drugs might be developed to selectively interfere with a subset of Plk1 functions...
  24. ncbi Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome
    Martina Casenghi
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 118:5101-8. 2005
    ..These findings uncover a mechanism through which Plk1 helps to coordinate changes in MT organisation with cell cycle progression, by controlling the dynein-dynactin-dependent transport of centrosomal proteins...
  25. pmc Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis
    Tomoya Yamaguchi
    Division of Biochemistry, Aichi Cancer Center Research Institute, Aichi 464 8681, Japan
    J Cell Biol 171:431-6. 2005
    ..Taken together, these results indicated a novel mechanism that Cdk1 regulated mitotic vimentin phosphorylation via not only a direct enzyme reaction but also Plk1 recruitment to vimentin...
  26. ncbi Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis
    Megan Fabbro
    Queensland Institute of Medical Research, P O Royal Brisbane Hospital, Brisbane, Queensland 4029, Australia
    Dev Cell 9:477-88. 2005
    ..These results highlight the centrosome as a site to organize phosphorylation of Cep55, enabling it to relocate to the midbody to function in mitotic exit and cytokinesis...
  27. ncbi Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission
    Ravindra B Chalamalasetty
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 119:3008-19. 2006
    ..This failure could be correlated with the persistence of these fragments at structures surrounding the midbody, suggesting that abscission requires the displacement of Ect2 from the contractile ring and its re-import into the nucleus...
  28. ncbi Structure of the N-terminal domain of the FOP (FGFR1OP) protein and implications for its dimerization and centrosomal localization
    Aleksandra Mikolajka
    Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Mol Biol 359:863-75. 2006
    ..The central part of the dimer contains the LisH domain. We further determined that the FOP LisH domain is part of a longer N-terminal segment that is required, albeit not sufficient, for dimerization and centrosomal localization of FOP...
  29. doi The spindle protein CHICA mediates localization of the chromokinesin Kid to the mitotic spindle
    Anna Santamaria
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Curr Biol 18:723-9. 2008
    ..We conclude that CHICA represents a novel interaction partner of the chromokinesin Kid that is required for the generation of polar ejection forces and chromosome congression...
  30. ncbi Comparative conservation analysis of the human mitotic phosphoproteome
    Rainer Malik
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried D 82152, Germany
    Bioinformatics 24:1426-32. 2008
    ....
  31. ncbi Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together
    A Arockia Jeyaprakash
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 131:271-85. 2007
    ..Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization...
  32. pmc Tension-sensitive Plk1 phosphorylation on BubR1 regulates the stability of kinetochore microtubule interactions
    Sabine Elowe
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Genes Dev 21:2205-19. 2007
    ....
  33. pmc Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis
    Anna Santamaria
    Department of Cell Biology and Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
    Mol Biol Cell 18:4024-36. 2007
    ..Finally, we show that Plk1 activity is essential for cleavage furrow formation and ingression, leading to successful cytokinesis...
  34. pmc Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity
    Kerstin H Thein
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    J Cell Biol 178:345-54. 2007
    ..We suggest that astrin contributes to the regulatory network that controls separase activity...
  35. ncbi Choice of Plk1 docking partners during mitosis and cytokinesis is controlled by the activation state of Cdk1
    Rüdiger Neef
    Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
    Nat Cell Biol 9:436-44. 2007
    ..The activation state of Cdk1, therefore, controls the switch of Plk1 localization from centrosomes and kinetochores during metaphase, to the central spindle during anaphase...
  36. ncbi Centrosome-associated NDR kinase regulates centrosome duplication
    Alexander Hergovich
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Mol Cell 25:625-34. 2007
    ..Overall, considering that centrosome overduplication is linked to cellular transformation, our observations may also provide a molecular link between mammalian NDR kinases and cancer...
  37. ncbi PICH, a centromere-associated SNF2 family ATPase, is regulated by Plk1 and required for the spindle checkpoint
    Christoph Baumann
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 128:101-14. 2007
    ..These data identify PICH as a novel essential component of checkpoint signaling. We propose that PICH binds to catenated centromere-related DNA to monitor tension developing between sister kinetochores...
  38. pmc Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2
    Anja Hanisch
    Department of Cell Biology, Max Planck Institute for Biochemistry, Martinsried, Germany
    EMBO J 25:5504-15. 2006
    ..These data suggest that the Ska1/2 complex plays a critical role in the maintenance of the metaphase plate and/or spindle checkpoint silencing...
  39. ncbi Origins and consequences of centrosome aberrations in human cancers
    Erich A Nigg
    Department of Cell Biology, Max Planck Institute for Biochemistry, Martinsried, Germany
    Int J Cancer 119:2717-23. 2006
    ..This review addresses the origins of centrosome aberrations in human tumors as well as the expected impact of centrosome aberrations on cell fate and tumor development...
  40. pmc Depletion of licensing inhibitor geminin causes centrosome overduplication and mitotic defects
    Kiku e K Tachibana
    Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK
    EMBO Rep 6:1052-7. 2005
    ..These results show that the consequences of geminin loss exceed its immediate role in DNA replication and extend to promoting chromosome mis-segregation in mitosis...
  41. pmc Coordinate regulation of the mother centriole component nlp by nek2 and plk1 protein kinases
    Joseph Rapley
    Department of Biochemistry, University of Leicester, University Rd, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 25:1309-24. 2005
    ..We also propose that phosphorylation by Nek2 may prime Nlp for phosphorylation by Plk1...
  42. pmc The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles
    Giulia Guarguaglini
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152, Martinsried, Germany
    Mol Biol Cell 16:1095-107. 2005
    ..We show that Cep170 labeling can be used to discriminate bona fide centriole overduplication from centriole amplification that results from aborted cell division...
  43. pmc Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis
    Reiko Honda
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Mol Biol Cell 14:3325-41. 2003
    ..A nonphosphorylatable mutant (TSS893-895AAA) was a poor activator of Aurora B, demonstrating that INCENP phosphorylation is important for kinase activation...
  44. ncbi Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation
    Martina Casenghi
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Dev Cell 5:113-25. 2003
    ....
  45. ncbi Regulation of cell division: stop the SIN!
    Ulrike Gruneberg
    Max Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Trends Cell Biol 13:159-62. 2003
    ..A novel mechanism, centered on the Polo-like kinase Plo1p and Dma1p - a protein with a RING finger and an FHA-domain - prevents cytokinesis as long as the spindle checkpoint is active...
  46. pmc Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint
    Anja Groth
    Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    EMBO J 22:1676-87. 2003
    ....
  47. ncbi Signal transduction. Capturing polo kinase
    Herman H W Sillje
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Science 299:1190-1. 2003
  48. ncbi Active cyclin B1-Cdk1 first appears on centrosomes in prophase
    Mark Jackman
    Wellcome Trust Cancer Research UK Institute and Department of Zoology, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Cell Biol 5:143-8. 2003
    ..We conclude that cyclin B1-Cdk1 is first activated in the cytoplasm and that centrosomes may function as sites of integration for the proteins that trigger mitosis...
  49. ncbi Identification of phosphorylation sites in the polo-like kinases Plx1 and Plk1 by a novel strategy based on element and electrospray high resolution mass spectrometry
    Mathias Wind
    Central Spectroscopy, German Cancer Research Center, Heidelberg, Germany
    Proteomics 2:1516-23. 2002
    ..Element mass spectrometry with phosphorus detection provides a quantitative phosphorylation profile of all phosphorylation sites accessible by LC...
  50. ncbi Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2
    Silvia Martin-Lluesma
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Science 297:2267-70. 2002
    ..Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint-deficient cells...
  51. ncbi The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles
    Thibault Mayor
    Department of Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Cell Sci 115:3275-84. 2002
    ..We conclude that the dissociation of C-Nap1 from mitotic centrosomes is regulated by localized phosphorylation rather than generalized proteolysis...
  52. ncbi The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase
    Izabela Sumara
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Mol Cell 9:515-25. 2002
    ..Cohesin phosphorylation depends on Polo-like kinase and reduces the ability of cohesin to bind to chromatin. These results suggest that Polo-like kinase regulates the dissociation of cohesin from chromosomes early in mitosis...
  53. pmc Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication
    Volker M Stucke
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 21:1723-32. 2002
    ..In contrast, centrosome (re-)duplication as well as cell division occur in the absence of hMps1. We conclude that hMps1 is required for the spindle assembly checkpoint but not for centrosome duplication...
  54. pmc Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway
    Jill A Mello
    Institut Curie, Research Section, UMR 218 du Centre National de la Recherche Scientifique CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    EMBO Rep 3:329-34. 2002
    ..A model is proposed in which the synergism between hAsf1 and CAF-1 for nucleosome formation during DNA repair is achieved through a transient physical interaction allowing histone delivery from Asf1 to CAF-1...
  55. ncbi Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensation
    Hidemasa Goto
    Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi464 8681, Japan
    Genes Cells 7:11-7. 2002
    ..Aurora B was recently demonstrated to be responsible for Ser10 phosphorylation in S. cerevisiae, C. elegans, Drosophila and Xenopus egg extract...
  56. pmc Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis
    Rüdiger Neef
    Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Biol 162:863-75. 2003
    ..We propose that phosphorylation of MKlp2 by Plk1 is necessary for the spatial restriction of Plk1 to the central spindle during anaphase and telophase, and the complex of these two proteins is required for cytokinesis...
  57. ncbi Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1
    Darren R Krause
    Signal Transduction Lab, Cancer and Cell Biology Division, Queensland Institute of Medical Research, 300 Herston Rd, QLD 4029, Australia
    Oncogene 22:5927-37. 2003
    ..Overall, we propose that ATM activation is not linked solely to DSBs and that ATM participates in initiating signaling pathways in response to replication block and UV-induced DNA damage...
  58. ncbi Proteome analysis of the human mitotic spindle
    Guido Sauer
    Max Planck Institute of Biochemistry, Department of Cell Biology, D 82152 Martinsried, Germany
    Mol Cell Proteomics 4:35-43. 2005
    ..This study illustrates the strength of a proteomic approach for the analysis of isolated human spindles and identifies several novel spindle components for future functional studies...
  59. ncbi Aurora-C kinase is a novel chromosomal passenger protein that can complement Aurora-B kinase function in mitotic cells
    Kaori Sasai
    Department of Molecular Pathology, Division of Pathology and Laboratory Medicine, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cell Motil Cytoskeleton 59:249-63. 2004
    ..Finally, Aurora-C could rescue the Aurora-B silenced multinucleation phenotype, demonstrating that Aurora-C kinase function overlaps with and complements Aurora-B kinase function in mitosis...
  60. ncbi Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase
    Alwin Krämer
    Danish Cancer Society, Institute of Cancer Biology, Department of Cell Cycle and Cancer, Strandboulevarden 49, DK 2100 Copenhagen Ø, Denmark
    Nat Cell Biol 6:884-91. 2004
    ....
  61. ncbi Cell cycle regulation of central spindle assembly
    Masanori Mishima
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Nature 430:908-13. 2004
    ..Thus, phosphoregulation of the motor domain of MKLP1 kinesin ensures that central spindle assembly occurs at the appropriate time in the cell cycle and maintains genomic stability...
  62. pmc Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    J Cell Biol 166:167-72. 2004
    ..We propose that MKlp2 is involved in the localization of Plk1, Aurora B, and Cdc14A to the central spindle during anaphase, and that the integration of signaling by these proteins is necessary for proper cytokinesis...
  63. pmc Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle
    Reto Gassmann
    Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Kings Buildings, Mayfield Rd, Edinburgh EH9 3JR, Scotland, UK
    J Cell Biol 166:179-91. 2004
    ..These results implicate the chromosomal passenger holocomplex in the maintenance of spindle integrity and suggest that histone H3 serine10 phosphorylation is performed by an Aurora B-INCENP subcomplex...
  64. ncbi Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1
    Volker M Stucke
    Department of Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Chromosoma 113:1-15. 2004
    ..Our data indicate that the catalytic domain of hMps1 displays affinity for microtubules and that microtubule binding could contribute to the regulation of kinase activity...
  65. ncbi Polo-like kinases and the orchestration of cell division
    Francis A Barr
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 5:429-40. 2004
  66. pmc Sgt1 is required for human kinetochore assembly
    Peter Steensgaard
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    EMBO Rep 5:626-31. 2004
    ..Our studies implicate Sgt1 as an essential protein and a critical assembly factor for the mammalian kinetochore, and lend credit to the hypothesis of a kinetochore assembly pathway that is conserved from yeast to man...
  67. ncbi Regulation of Aurora-A kinase on the mitotic spindle
    Thomas A Kufer
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany
    Chromosoma 112:159-63. 2003
    ..Here, we review these findings with particular emphasis on the role of TPX2, a prominent spindle component implicated in a Ran-GTP-mediated spindle assembly pathway...
  68. pmc The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex
    Kin Yip Cheng
    Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO J 22:5757-68. 2003
    ..Most of these residues had been shown to be important for biological activity through mutational studies. The results provide an explanation for phospho-peptide recognition and create the basis for new functional studies...
  69. pmc Aurora-A overexpression reveals tetraploidization as a major route to centrosome amplification in p53-/- cells
    Patrick Meraldi
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    EMBO J 21:483-92. 2002
    ..We propose that errors during cell division, combined with the inability to detect the resulting hyperploidy, constitute a major cause for numerical centrosome aberrations in tumors...