B Nieswandt

Summary

Country: Germany

Publications

  1. ncbi Platelets in atherothrombosis: lessons from mouse models
    B Nieswandt
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 3:1725-36. 2005
  2. ncbi The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction
    David Varga-Szabo
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, 97078 Wurzburg, Germany
    J Exp Med 205:1583-91. 2008
  3. ncbi Combined In Vivo Depletion of Glycoprotein VI and C-Type Lectin-Like Receptor 2 Severely Compromises Hemostasis and Abrogates Arterial Thrombosis in Mice
    Markus Bender
    Chair of Vascular Medicine, Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University Clinic Würzburg, Josef Schneider Str, 2, 97080 Wurzburg, Germany
    Arterioscler Thromb Vasc Biol 33:926-34. 2013
  4. ncbi CLP36 is a negative regulator of glycoprotein VI signaling in platelets
    Shuchi Gupta
    Vascular Medicine, Rudolf Virchow Center, University of Wurzburg, Josef Schneider St 2, D15, 97080 Wurzburg, Germany
    Circ Res 111:1410-20. 2012
  5. ncbi Integrins in platelet activation
    B Nieswandt
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 7:206-9. 2009
  6. ncbi Platelet-collagen interaction: is GPVI the central receptor?
    Bernhard Nieswandt
    Department of Vascular Biology, Rudolf Virchow Center for Experimental Biomedicine Versbacher, Wurzburg, Germany
    Blood 102:449-61. 2003
  7. ncbi Ischaemic stroke: a thrombo-inflammatory disease?
    Bernhard Nieswandt
    University Hospital Wurzburg, Rudolf Virchow Centre, DFG Research Centre for Experimental Biomedicine, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    J Physiol 589:4115-23. 2011
  8. ncbi Costimulation of Gi- and G12/G13-mediated signaling pathways induces integrin alpha IIbbeta 3 activation in platelets
    Bernhard Nieswandt
    Rudolf Virchow Center for Experimental Biomedicine, University of Wurzburg, 97078 Wurzburg, Germany
    J Biol Chem 277:39493-8. 2002
  9. ncbi Platelet adhesion and activation mechanisms in arterial thrombosis and ischaemic stroke
    B Nieswandt
    Vascular Medicine, University Hospital Würzburg and Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 9:92-104. 2011
  10. ncbi Arterial thrombus formation. Novel mechanisms and targets
    I Hagedorn
    University Hospital Wurzburg, Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    Hamostaseologie 30:127-35. 2010

Collaborators

Detail Information

Publications69

  1. ncbi Platelets in atherothrombosis: lessons from mouse models
    B Nieswandt
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 3:1725-36. 2005
    ..This review will summarize key findings made by these approaches and discuss them in the context of human disease...
  2. ncbi The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction
    David Varga-Szabo
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, 97078 Wurzburg, Germany
    J Exp Med 205:1583-91. 2008
    ..These results establish STIM1 as an important mediator in the pathogenesis of ischemic cardio- and cerebrovascular events...
  3. ncbi Combined In Vivo Depletion of Glycoprotein VI and C-Type Lectin-Like Receptor 2 Severely Compromises Hemostasis and Abrogates Arterial Thrombosis in Mice
    Markus Bender
    Chair of Vascular Medicine, Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University Clinic Würzburg, Josef Schneider Str, 2, 97080 Wurzburg, Germany
    Arterioscler Thromb Vasc Biol 33:926-34. 2013
    ..These findings may have important implications of the potential use of anti-GPVI and anti-CLEC-2-based agents in the prevention of thrombotic diseases...
  4. ncbi CLP36 is a negative regulator of glycoprotein VI signaling in platelets
    Shuchi Gupta
    Vascular Medicine, Rudolf Virchow Center, University of Wurzburg, Josef Schneider St 2, D15, 97080 Wurzburg, Germany
    Circ Res 111:1410-20. 2012
    ..GPVI signaling has to be tightly regulated to prevent uncontrolled intravascular platelet activation, but the underlying mechanisms are not fully understood...
  5. ncbi Integrins in platelet activation
    B Nieswandt
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 7:206-9. 2009
    ....
  6. ncbi Platelet-collagen interaction: is GPVI the central receptor?
    Bernhard Nieswandt
    Department of Vascular Biology, Rudolf Virchow Center for Experimental Biomedicine Versbacher, Wurzburg, Germany
    Blood 102:449-61. 2003
    ..This review discusses these recent developments and proposes possible mechanisms for how GPVI acts in concert with other receptors and signaling pathways to initiate hemostasis and arterial thrombosis...
  7. ncbi Ischaemic stroke: a thrombo-inflammatory disease?
    Bernhard Nieswandt
    University Hospital Wurzburg, Rudolf Virchow Centre, DFG Research Centre for Experimental Biomedicine, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    J Physiol 589:4115-23. 2011
    ..This review summarizes these recent developments and briefly discusses their potential clinical impact...
  8. ncbi Costimulation of Gi- and G12/G13-mediated signaling pathways induces integrin alpha IIbbeta 3 activation in platelets
    Bernhard Nieswandt
    Rudolf Virchow Center for Experimental Biomedicine, University of Wurzburg, 97078 Wurzburg, Germany
    J Biol Chem 277:39493-8. 2002
    ....
  9. ncbi Platelet adhesion and activation mechanisms in arterial thrombosis and ischaemic stroke
    B Nieswandt
    Vascular Medicine, University Hospital Würzburg and Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 9:92-104. 2011
    ..This review summarises recent developments in understanding the principal platelet adhesion receptor systems with a focus on their involvement in arterial thrombosis and ischaemic stroke models...
  10. ncbi Arterial thrombus formation. Novel mechanisms and targets
    I Hagedorn
    University Hospital Wurzburg, Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    Hamostaseologie 30:127-35. 2010
    ..The suitability of these pathways as novel targets for antithrombotic therapy is discussed...
  11. ncbi Platelet hyperreactivity and a prothrombotic phenotype in mice with a gain-of-function mutation in phospholipase Cgamma2
    M Elvers
    Chair of Vascular Medicine, University Clinic, and Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Germany
    J Thromb Haemost 8:1353-63. 2010
    ..In contrast, PLCgamma2 becomes activated downstream of receptors that interact with immobilized ligands such as the collagen receptor glycoprotein (GP) VI or activated integrins...
  12. ncbi Defective diacylglycerol-induced Ca2+ entry but normal agonist-induced activation responses in TRPC6-deficient mouse platelets
    G Ramanathan
    Chair of Vascular Medicine, DFG Research Center for Experimental Biomedicine, University Hospital and Rudolf Virchow Center, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 10:419-29. 2012
    ....
  13. ncbi The SLAM family member CD84 is regulated by ADAM10 and calpain in platelets
    S Hofmann
    Chair of Vascular Medicine, University of Wurzburg, University Hospital and Rudolf Virchow Center for Experimental Biomedicine, Wurzburg, Germany
    J Thromb Haemost 10:2581-92. 2012
    ..Because of its ability to form homodimers, CD84 has been suggested to mediate contact-dependent signaling and contribute to thrombus stability. However, nothing is known about the cellular regulation of CD84...
  14. ncbi Enhanced cortical reperfusion protects coagulation factor XII-deficient mice from ischemic stroke as revealed by high-field MRI
    M Pham
    Department of Neuroradiology, University of Heidelberg, Germany Department of Neuroradiology, University of Wurzburg, Germany
    Neuroimage 49:2907-14. 2010
    ..99% (FXII(-/-), p=0.17). Deficiency of FXII allows neocortical reperfusion after tMCAO and rescues brain tissue by this mechanism. This study supports the concept of FXII as a promising new target for stroke prevention and therapy...
  15. ncbi The role of glycoprotein Ibalpha and von Willebrand factor interaction in stroke development
    G Stoll
    Department of Neurology, University of Wurzburg, Germany
    Hamostaseologie 30:136-8. 2010
    ..In conclusion, there is compelling evidence that GPIbalpha/VWF interactions and downstream signaling via phospholipase D1 (PLD1) provide new therapeutic targets in ischemic stroke...
  16. ncbi Calcium signaling in platelets
    D Varga-Szabo
    Chair of Vascular Medicine and Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 7:1057-66. 2009
    ..This review summarizes the current knowledge of platelet Ca2+ homeostasis with a focus on the newly identified Ca2+ entry mechanisms...
  17. ncbi Diverging signaling events control the pathway of GPVI down-regulation in vivo
    Tamer Rabie
    University of Wurzburg, Rudolf Virchow Center, Deutsche Forschungsgemeinschaft Research Center for Experimental Biomedicine, Wurzburg, Germany
    Blood 110:529-35. 2007
    ..These results reveal the existence of 2 distinct signaling pathways downstream of the FcRgamma-ITAM and show that it is possible to uncouple GPVI down-regulation from undesired side effects with obvious therapeutic implications...
  18. ncbi Dual role of platelet protein kinase C in thrombus formation: stimulation of pro-aggregatory and suppression of procoagulant activity in platelets
    Amrei Strehl
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, 6200 MD Maastricht, The Netherlands
    J Biol Chem 282:7046-55. 2007
    ..This platelet signaling protein is the first one identified to balance the pro-aggregatory and procoagulant functions of thrombi...
  19. ncbi Two-phase antithrombotic protection after anti-glycoprotein VI treatment in mice
    Valerie Schulte
    Rudolf-Virchow-Zentrum, , Germany
    Arterioscler Thromb Vasc Biol 26:1640-7. 2006
    ..CONCLUSIONS: Anti-GPVI treatment induces 2-phase antithrombotic protection in mice consisting of a partial and transient inhibition of thrombin responses in platelets and a prolonged and complete loss of the collagen response...
  20. ncbi Targeting coagulation factor XII provides protection from pathological thrombosis in cerebral ischemia without interfering with hemostasis
    Christoph Kleinschnitz
    Department of Neurology, Institute for Clinical Biochemistry and Pathobiochemistry, University of Wurzburg, 97080 Wurzburg, Germany
    J Exp Med 203:513-8. 2006
    ..As FXII appears to be instrumental in pathologic fibrin formation but dispensable for hemostasis, FXII inhibition may offer a selective and safe strategy for preventing stroke and other thromboembolic diseases...
  21. ncbi Reduced thrombus stability in mice lacking the alpha2A-adrenergic receptor
    Miroslava Pozgajova
    Rudolf Virchow Center, Deutsche Forschungsgemeinschaft DFG Center for Experimental Biomedicine, the Institute of Pharmacology and Toxicology, University of Wurzburg, Germany
    Blood 108:510-4. 2006
    ..These results demonstrate that alpha(2A) plays a significant role in thrombus stabilization...
  22. ncbi Targeting of the collagen-binding site on glycoprotein VI is not essential for in vivo depletion of the receptor
    Valerie Schulte
    Rudolf Virchow Center for Experimental Biomedicine, , , Germany
    Blood 101:3948-52. 2003
    ..This indicates that anti-GPVI agents, irrespective of their binding site may generally induce down-regulation of the receptor in vivo...
  23. ncbi GATA1-mediated megakaryocyte differentiation and growth control can be uncoupled and mapped to different domains in GATA1
    Christiane Kuhl
    Department of Hematology, Weatherall Institute of Molecular Medicine, University of Oxford and John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
    Mol Cell Biol 25:8592-606. 2005
    ..Thus, distinct GATA1 domains regulate terminal megakaryocyte gene expression leading to platelet release and restrain megakaryocyte growth, and these processes can be uncoupled...
  24. ncbi In vivo thrombus formation in murine models
    Ulrich J H Sachs
    Rudolf Virchow Center, Deutsche Forschungsgemeinschaft Research Center for Experimental Biomedicine, University of Wurzburg, Germany
    Circ Res 100:979-91. 2007
    ..This review summarizes in vivo findings that contributed significantly to our understanding of hemostatic and thrombotic processes and which may help to guide future research...
  25. ncbi Targeting platelets in acute experimental stroke: impact of glycoprotein Ib, VI, and IIb/IIIa blockade on infarct size, functional outcome, and intracranial bleeding
    Christoph Kleinschnitz
    Department of Neurology, University of Wurzburg, Wurzburg, Germany
    Circulation 115:2323-30. 2007
    ..In the present study, we addressed the pathogenic role of GPIb, GPVI, and the aggregation receptor GPIIb/IIIa in experimental stroke in mice...
  26. ncbi Diminished thrombus formation and alleviation of myocardial infarction and reperfusion injury through antibody- or small-molecule-mediated inhibition of selectin-dependent platelet functions
    Gertie J Oostingh
    University of Wurzburg, Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, Wurzburg Germany
    Haematologica 92:502-12. 2007
    ..P-selectin ctin has been implicated in important platelet functions. However, neither its role in thrombus formation and cardiovascular disorders nor its suitability as a therapeutic target structure is entirely clear...
  27. ncbi Role of murine integrin alpha2beta1 in thrombus stabilization and embolization: contribution of thromboxane A2
    Marijke J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    Thromb Haemost 98:1072-80. 2007
    ..The increased embolization in alpha2-null mice may argue against the use of alpha2beta1 integrin inhibitors for antithrombotic therapy...
  28. ncbi Loss of talin1 in platelets abrogates integrin activation, platelet aggregation, and thrombus formation in vitro and in vivo
    Bernhard Nieswandt
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, 97078 Wurzburg, Germany
    J Exp Med 204:3113-8. 2007
    ..Collectively, these experiments demonstrate that talin is required for inside-out activation of platelet integrins in hemostasis and thrombosis...
  29. ncbi Cell adhesion mechanisms in platelets
    David Varga-Szabo
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, Zinklesweg 10, 97078 Wurzburg, Germany
    Arterioscler Thromb Vasc Biol 28:403-12. 2008
    ..This review summarizes the most important structural and functional properties of these adhesion receptors and briefly discusses their potential as targets for antithrombotic therapy...
  30. ncbi Blocking of platelets or intrinsic coagulation pathway-driven thrombosis does not prevent cerebral infarctions induced by photothrombosis
    Christoph Kleinschnitz
    Department of Neurology, University of Wurzburg, Germany
    Stroke 39:1262-8. 2008
    ..Although the ensuing activation of platelets is well established, their contribution for thrombosis and tissue damage has not formally been proved...
  31. ncbi Molecular mechanisms of thrombus formation in ischemic stroke: novel insights and targets for treatment
    Guido Stoll
    Department of Neurology, University of Wurzburg, Wurzburg, Germany
    Blood 112:3555-62. 2008
    ..This review discusses recent advances related to antithrombotic strategies in experimental stroke research...
  32. ncbi Platelet-derived CD154 enables T-cell priming and protection against Listeria monocytogenes challenge
    Bennett D Elzey
    Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA
    Blood 111:3684-91. 2008
    ....
  33. ncbi Effects of estrogen replacement therapies on mouse platelet function and glycoprotein VI levels
    Xing-Hong Leng
    Medicine/Thrombosis Research, Baylor College of Medicine, Houston, Texas 77030, USA
    Circ Res 97:415-7. 2005
    ..The GPVI collagen receptor likely mediated some of these effects, because the ERT regimens induced changes in platelet surface GPVI expression corresponding to the observed platelet activation...
  34. ncbi Unresponsiveness of platelets lacking both Galpha(q) and Galpha(13). Implications for collagen-induced platelet activation
    Alexandra Moers
    Pharmakologisches Institut, Universitat Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany
    J Biol Chem 279:45354-9. 2004
    ....
  35. ncbi Facilitating roles of murine platelet glycoprotein Ib and alphaIIbbeta3 in phosphatidylserine exposure during vWF-collagen-induced thrombus formation
    Marijke J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    J Physiol 558:403-15. 2004
    ..Hence, these glycoproteins have an assistant procoagulant role in collagen-dependent thrombus formation, which is most prominent at reduced GPVI activity and is independent of the presence of thrombin...
  36. ncbi GPVI down-regulation in murine platelets through metalloproteinase-dependent shedding
    Wolfgang Bergmeier
    The Center for Blood Research, Institute for Biomedical Research, Department of Pathology, Harvard Medical School, Boston, Massachusetts, UDA
    Thromb Haemost 91:951-8. 2004
    ....
  37. ncbi Genetic variation responsible for mouse strain differences in integrin alpha 2 expression is associated with altered platelet responses to collagen
    Tong Tong Li
    Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    Blood 103:3396-402. 2004
    ..These strain differences serve as a useful model for the 2-fold difference in human platelet alpha(2)beta(1) expression and demonstrate that alpha(2)beta(1) participates in signaling during platelet activation...
  38. ncbi G13 is an essential mediator of platelet activation in hemostasis and thrombosis
    Alexandra Moers
    Institute of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany
    Nat Med 9:1418-22. 2003
    ..We conclude that G(13)-mediated signaling processes are required for normal hemostasis and thrombosis and may serve as a new target for antiplatelet drugs...
  39. ncbi Multiple integrin-ligand interactions synergize in shear-resistant platelet adhesion at sites of arterial injury in vivo
    Sabine Grüner
    Rudolf Virchow Center for Experimental Biomedicine, University of Wurzburg, Versbacher Str 9, 97078 Wurzburg, Germany
    Blood 102:4021-7. 2003
    ..We conclude that shear-resistant platelet adhesion on the injured vessel wall in vivo is a highly integrated process involving multiple integrin-ligand interactions, none of which by itself is essential...
  40. ncbi Complementary roles of glycoprotein VI and alpha2beta1 integrin in collagen-induced thrombus formation in flowing whole blood ex vivo
    Marijke J E Kuijpers
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    FASEB J 17:685-7. 2003
    ....
  41. ncbi A crucial role of glycoprotein VI for platelet recruitment to the injured arterial wall in vivo
    Steffen Massberg
    Deutsches Herzzentrum und 1 Medizinische Klinik, Technische Universitat Munchen, D 80636 München, Germany
    J Exp Med 197:41-9. 2003
    ....
  42. ncbi A critical role of platelet adhesion in the initiation of atherosclerotic lesion formation
    Steffen Massberg
    Deutsches Herzzentrum und 1 Medizinische Klinik, Technische Universitat Munchen, Germany
    J Exp Med 196:887-96. 2002
    ..These findings establish the platelet as a major player in initiation of the atherogenetic process...
  43. ncbi Differential effects of reduced glycoprotein VI levels on activation of murine platelets by glycoprotein VI ligands
    Daniel C Snell
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, U.K
    Biochem J 368:293-300. 2002
    ..The clinical significance of these results is discussed...
  44. ncbi Absence of GPIbalpha is responsible for aberrant membrane development during megakaryocyte maturation: ultrastructural study using a transgenic model
    Christel Poujol
    UMR 5533 CNRS, Laboratoire d Hematologie, Hopital Cardiologique, Avenue de Magellan, 33604 Pessac, France
    Exp Hematol 30:352-60. 2002
    ..To study how giant platelets are produced in vivo, we used a model of GPIbalpha-deficient mice (GPIbalpha(null)) and mice rescued with the human GPIbalpha transgene (GPIbalpha(null;hTg))...
  45. ncbi Anti-glycoprotein VI treatment severely compromises hemostasis in mice with reduced alpha2beta1 levels or concomitant aspirin therapy
    Sabine Grüner
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Wurzburg, Wurzburg, Germany
    Circulation 110:2946-51. 2004
    ..The activating platelet collagen receptor, glycoprotein VI (GP VI), may serve as a powerful antithrombotic target because its inhibition or absence results in profound protection against arterial thrombosis but no major bleeding in mice...
  46. ncbi Relative antithrombotic effect of soluble GPVI dimer compared with anti-GPVI antibodies in mice
    Sabine Grüner
    Rudolf Virchow Center, Deutsch Forschungsgemeinschaft Research Center for Experimental Biomedicine, University of Wurzburg, 97078 Wurzburg, Germany
    Blood 105:1492-9. 2005
    ..This indicates that direct targeting of GPVI provides significantly stronger protection against arterial thrombosis than soluble GPVI dimer...
  47. ncbi Integrin alpha 2-deficient mice develop normally, are fertile, but display partially defective platelet interaction with collagen
    Olaf Holtkötter
    Department of Dermatology, University of Cologne, Cologne, Germany
    J Biol Chem 277:10789-94. 2002
    ..Thus, alpha(2)beta(1) plays a supportive rather than an essential role in platelet-collagen interactions. These results are in agreement with the observation that alpha(2)beta(1)-deficient animals suffer no bleeding anomalies...
  48. ncbi Human atheromatous plaques stimulate thrombus formation by activating platelet glycoprotein VI
    Sandra Penz
    Institute for Prevention of Cardiovascular Diseases, University of Munich, Munich, Germany
    FASEB J 19:898-909. 2005
    ..This platelet collagen receptor, essential for plaque-induced thrombus formation, presents a promising new anti-thrombotic target for the prevention of ischemic cardiovascular diseases...
  49. ncbi The glycoprotein VI-phospholipase Cgamma2 signaling pathway controls thrombus formation induced by collagen and tissue factor in vitro and in vivo
    Imke C A Munnix
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 25:2673-8. 2005
    ..We investigated the signaling pathway of collagen-induced platelet activation in interaction with tissue factor-triggered coagulation during the thrombus-forming process...
  50. ncbi Evidence for a role of ADAM17 (TACE) in the regulation of platelet glycoprotein V
    Tamer Rabie
    Vascular Biology, Rudolf Virchow Center, Deutsche Forschungsgemeinschaft Research Center for Experimental Biomedicine, University of Wurzburg, 97078 Wurzburg, Germany
    J Biol Chem 280:14462-8. 2005
    ..These results demonstrate that GPV is cleaved upon agonist-induced platelet activation and show that ADAM17 is the major enzyme mediating this process...
  51. ncbi Diminished lymphocyte adhesion and alleviation of allergic responses by small-molecule- or antibody-mediated inhibition of L-selectin functions
    Gertie J Oostingh
    Rudolf Virchow Center, and Department of Dermatology, Julius Maximilians University, Wurzburg, Germany
    J Invest Dermatol 127:90-7. 2007
    ..0002 and P=0.0001, respectively). Our data demonstrate that it is possible, in principle, to diminish T-cell-mediated allergic reactions through interference with L-selectin functions during the early sensitization phase...
  52. ncbi Platelets promote coagulation factor XII-mediated proteolytic cascade systems in plasma
    Julia Johne
    1. Institute of Clinical Biochemistry and Pathobiochemistry, Josef-Schneider-Strasse 2, , , Germany
    Biol Chem 387:173-8. 2006
    ..In summary, our data suggest that FXII-initiated protease cascades may proceed on platelet surfaces, with implications for inflammation and clotting...
  53. ncbi The intrinsic pathway of coagulation is essential for thrombus stability in mice
    Thomas Renne
    Institute for Clinical Biochemistry and Pathobiochemistry, Julius Maximilians University Würzburg, Josef Schneider Strasse 2, D 97080 Wurzburg, Germany
    Blood Cells Mol Dis 36:148-51. 2006
    ..These findings demonstrate that FXII-mediated fibrin formation is crucial for pathological arterial thrombosis but not for hemostasis and suggest that FXII could be an ideal target for safe anticoagulation...
  54. ncbi Understanding platelets. Lessons from proteomics, genomics and promises from network analysis
    Marcus Dittrich
    Department of Bioinformatics, Biocenter, , , Germany
    Thromb Haemost 94:916-25. 2005
    ..Understanding the surprisingly complex platelet regarding compartmentalization, key cascades, and pathways including clinical implications will remain an exciting and hopefully fruitful challenge for the future...
  55. ncbi BclxL overexpression in megakaryocytes leads to impaired platelet fragmentation
    Yulia Kaluzhny
    Department of Biochemistry and Whittaker Cardiovascular Institute, Boston University School of Medicine, MA 02118, USA
    Blood 100:1670-8. 2002
    ..Our study indicates that regulated expression of BclxL in megakaryocytes is important for the development of cells with a high potential to fragment into platelets...
  56. ncbi Defective thrombus formation in mice lacking coagulation factor XII
    Thomas Renne
    Institute of Clinical Biochemistry and Pathobiochemistry, Deutsche Forschungsgemeinschaft Research Center for Experimental Biomedicine, University of Wurzburg, 97078 Wurzburg, Germany
    J Exp Med 202:271-81. 2005
    ..The results establish FXII as essential for thrombus formation, and identify FXII as a novel target for antithrombotic therapy...
  57. ncbi Flow cytometric detection of activated mouse integrin alphaIIbbeta3 with a novel monoclonal antibody
    Wolfgang Bergmeier
    Department of Molecular Oncology, General Surgery, Witten/Herdecke University, Wuppertal, Germany
    Cytometry 48:80-6. 2002
    ..Furthermore, JON/A(FITC) and JON/A(PE) provide the first examples of fluorescent antibody derivatives with identical antigenic specificity that allow the discrimination between the resting and the activated state of an integrin...
  58. ncbi Aspirin induces platelet receptor shedding via ADAM17 (TACE)
    Barsom Aktas
    Rudolf Virchow Center for Experimental Biomedicine, Versbacherstrasse 9, 97078 Wurzburg, Germany
    J Biol Chem 280:39716-22. 2005
    ..These data demonstrate that aspirin at high concentrations induces shedding of GPIbalpha and GPV by an ADAM17-dependent mechanism and that this process can occur in vivo...
  59. ncbi Cooperation between platelet-derived CD154 and CD4+ T cells for enhanced germinal center formation
    Bennett D Elzey
    Department of Urology, Iowa City, IA 52242, USA
    J Leukoc Biol 78:80-4. 2005
    ..We propose that in the physiologic setting where antigen-specific B and T cells are rare, platelets function to enhance signals required for robust adaptive humoral immunity...
  60. ncbi Targeting of platelet integrin alphaIIbbeta3 determines systemic reaction and bleeding in murine thrombocytopenia regulated by activating and inhibitory FcgammaR
    Bernhard Nieswandt
    Department of Vascular Biology, Rudolf-Virchow-Zentrum, , , Germany
    Int Immunol 15:341-9. 2003
    ....
  61. ncbi The C-terminal peptide of thrombospondin-1 stimulates distinct signaling pathways but induces an activation-independent agglutination of platelets and other cells
    Simone Voit
    Institut fur Pharmakologie und Klinische Pharmakologie, Universitatsklinikum Dusseldorf, Moorenstr 5, Germany
    FEBS Lett 544:240-5. 2003
    ..Interestingly, 4N1-1 also stimulated activation-independent agglutination of different megakaryocytic and non-megakaryocytic cells. 4N1-1-induced cell agglutination but not platelet signaling was inhibited by anti-CD47 antibodies...
  62. ncbi Platelet NAD(P)H-oxidase-generated ROS production regulates alphaIIbbeta3-integrin activation independent of the NO/cGMP pathway
    Antonija Jurak Begonja
    Institut für Klinische Biochemie and Pathobiochemie, Josef Schneider Str 2, 97080 Wurzburg, Germany
    Blood 106:2757-60. 2005
    ..These inhibitors also reduced platelet aggregation and thrombus formation on collagen under high shear and achieved their effects independent of the nitric oxide/cyclic guanosine monophosphate (NO/cGMP) pathway...
  63. ncbi Metalloproteinase inhibitors improve the recovery and hemostatic function of in vitro-aged or -injured mouse platelets
    Wolfgang Bergmeier
    The CBR Institute for Biomedical Research, Inc, 800 Huntington Ave, Boston, MA 02115, USA
    Blood 102:4229-35. 2003
    ..Surface expression of GPIbalpha might be a powerful marker to determine the quality of platelet concentrates, because it reflects metalloproteinase activity in vitro...
  64. ncbi Enhanced in vivo platelet adhesion in vasodilator-stimulated phosphoprotein (VASP)-deficient mice
    Steffen Massberg
    Deutsches Herzzentrum und 1, Medizinische Klinik, Klinikum rechts der Isar, Technische Universitat Munchen, Munchen, Germany
    Blood 103:136-42. 2004
    ..These data show for the first time in vivo that VASP is involved in down-regulation of platelet adhesion to the vascular wall under both physiologic and pathophysiologic conditions...
  65. ncbi Kindlin-3 is essential for integrin activation and platelet aggregation
    Markus Moser
    Department of Molecular Medicine, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Med 14:325-30. 2008
    ..We have therefore identified Kindlin-3 as a novel and essential element for platelet integrin activation in hemostasis and thrombosis...
  66. ncbi Flow-cytometric analysis of mouse platelet function
    Bernhard Nieswandt
    Department of Vascular Biology, Rudolf Virchow Centre for Experimental Biomedicine, University of Wuerzburg, Germany
    Methods Mol Biol 272:255-68. 2004
  67. ncbi An EF hand mutation in Stim1 causes premature platelet activation and bleeding in mice
    Johannes Grosse
    Ingenium Pharmaceuticals AG, Munich, Germany
    J Clin Invest 117:3540-50. 2007
    ..These findings identify Stim1 as a central regulator of platelet function and suggest a cell type-specific activation or composition of the CRAC complex...
  68. ncbi Relative efficacy of intravenous immunoglobulin G in ameliorating thrombocytopenia induced by antiplatelet GPIIbIIIa versus GPIbalpha antibodies
    Michelle Lee Webster
    Canadian Blood Services, Ottawa, ON, Canada
    Blood 108:943-6. 2006
    ..These results were repeated in C57BL/6 mice, and with different IVIG preparations. These data in mice suggest that patients with ITP mediated by anti-GPIbalpha antibodies may be less responsive to IVIG treatment...
  69. ncbi Platelet-mediated modulation of adaptive immunity. A communication link between innate and adaptive immune compartments
    Bennett D Elzey
    The University of Iowa, Department of Urology, Iowa City, IA 52242, USA
    Immunity 19:9-19. 2003
    ..Additionally, depletion of platelets in normal mice results in decreased antigen-specific antibody production...