Peter Neubauer

Summary

Country: Germany

Publications

  1. doi request reprint Scale-down simulators for metabolic analysis of large-scale bioprocesses
    Peter Neubauer
    Technische Universitat Berlin, Institute of Biotechnology, Department of Bioprocess Engineering, Ackerstr 71 76, D 13355 Berlin, Germany
    Curr Opin Biotechnol 21:114-21. 2010
  2. pmc Complete genome sequence of the lytic Pseudomonas fluorescens phage ϕIBB-PF7A
    Sanna Sillankorva
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    Virol J 8:142. 2011
  3. pmc Heterologous production of active ribonuclease inhibitor in Escherichia coli by redox state control and chaperonin coexpression
    Juozas Siurkus
    Thermo Fisher Scientific, V Graiciuno 8, LT 02241 Vilnius, Lithuania
    Microb Cell Fact 10:65. 2011
  4. pmc Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures
    Maciej Pilarek
    Department of Biotechnology and Bioprocess Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Poland
    Microb Cell Fact 10:50. 2011
  5. pmc Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli
    Juozas Siurkus
    Thermo Fisher Scientific V A Graiciuno, Vilnius, Lithuania
    Microb Cell Fact 10:31. 2011
  6. pmc Fed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktis
    Boris Wilmes
    Institute of Marine Biotechnology, W Rathenau Str 49, D 17489 Greifswald, Germany
    Microb Cell Fact 9:72. 2010
  7. pmc Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks
    Johanna Panula-Perälä
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, P, O, Box 4300, FI 90014 Oulu, Finland
    Microb Cell Fact 7:31. 2008
  8. doi request reprint Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties
    Markus Alahuhta
    Biocenter Oulu, University of Oulu, Oulu, Finland
    Protein Eng Des Sel 21:257-66. 2008
  9. doi request reprint Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM
    Markus Alahuhta
    Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN 90014 University of Oulu, Finland
    Acta Crystallogr D Biol Crystallogr 64:178-88. 2008
  10. pmc High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer
    Kaisa Ukkonen
    BioSilta Oy, FI 90014 Oulu, Finland
    Microb Cell Fact 10:107. 2011

Collaborators

Detail Information

Publications44

  1. doi request reprint Scale-down simulators for metabolic analysis of large-scale bioprocesses
    Peter Neubauer
    Technische Universitat Berlin, Institute of Biotechnology, Department of Bioprocess Engineering, Ackerstr 71 76, D 13355 Berlin, Germany
    Curr Opin Biotechnol 21:114-21. 2010
    ..These simulators will pave the way for screening robust strains and process conditions...
  2. pmc Complete genome sequence of the lytic Pseudomonas fluorescens phage ϕIBB-PF7A
    Sanna Sillankorva
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    Virol J 8:142. 2011
    ..This work describes the complete genome sequence of this phage...
  3. pmc Heterologous production of active ribonuclease inhibitor in Escherichia coli by redox state control and chaperonin coexpression
    Juozas Siurkus
    Thermo Fisher Scientific, V Graiciuno 8, LT 02241 Vilnius, Lithuania
    Microb Cell Fact 10:65. 2011
    ..After optimisation of the production conditions in the shake flask scale the process was scaled up to high cell densities by applying a glucose limited fed-batch procedure...
  4. pmc Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures
    Maciej Pilarek
    Department of Biotechnology and Bioprocess Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Poland
    Microb Cell Fact 10:50. 2011
    ....
  5. pmc Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli
    Juozas Siurkus
    Thermo Fisher Scientific V A Graiciuno, Vilnius, Lithuania
    Microb Cell Fact 10:31. 2011
    ..The members of this group are difficult to produce in E. coli and other recombinant hosts due to their high aggregation tendency...
  6. pmc Fed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktis
    Boris Wilmes
    Institute of Marine Biotechnology, W Rathenau Str 49, D 17489 Greifswald, Germany
    Microb Cell Fact 9:72. 2010
    ..Here we present the first fed-batch cultivation strategy for this auspicious alternative expression host...
  7. pmc Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks
    Johanna Panula-Perälä
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, P, O, Box 4300, FI 90014 Oulu, Finland
    Microb Cell Fact 7:31. 2008
    ..abstract:..
  8. doi request reprint Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties
    Markus Alahuhta
    Biocenter Oulu, University of Oulu, Oulu, Finland
    Protein Eng Des Sel 21:257-66. 2008
    ..The binding of this citrate molecule is stabilized by hydrogen bonding interactions with the new binding groove...
  9. doi request reprint Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM
    Markus Alahuhta
    Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN 90014 University of Oulu, Finland
    Acta Crystallogr D Biol Crystallogr 64:178-88. 2008
    ..In the liganded structures of A178L and ml1A178L the structural differences induced by the mutation are minimal. Structural characterization of the ml1A178L variant highlights its structural plasticity...
  10. pmc High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer
    Kaisa Ukkonen
    BioSilta Oy, FI 90014 Oulu, Finland
    Microb Cell Fact 10:107. 2011
    ..Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved...
  11. ncbi request reprint Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracycline
    Antje Neubauer
    Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P O Box 5000, University of Oulu, FIN 90014 Oulu, Finland
    J Biotechnol 128:308-21. 2007
    ..The optimisation approach resulted in a fermentation yield of 143 mg L(-1) of active C-P4H, corresponding to approximately 7.5% of the total soluble cell protein...
  12. pmc Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system
    Maria Ruottinen
    Bioprocess Engineering Laboratory, Dept, of Process and Environmental Engineering, University of Oulu, P O Box 4300, FIN 90014 University of Oulu, Finland
    BMC Biotechnol 8:33. 2008
    ..The development of feeding protocols and the study of AOX1-controlled recombinant protein production have been largely made in shake flasks, although shake flasks have very limited possibilities for measurement and control...
  13. pmc A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures
    Mirja Krause
    BioSilta Oy, c o Department of Biochemistry, University of Oulu, Finland
    Microb Cell Fact 9:11. 2010
    ..In this article we describe the impact of a novel enzymatically controlled fed-batch cultivation technology on recombinant protein production in Escherichia coli in simple shaken cultures...
  14. doi request reprint Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides
    Xinrui Zhou
    Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Berlin, Germany
    FEBS J 280:1475-90. 2013
    ..Indeed, the prepared PNPs performed well in preliminary transglycosylation reactions resulting in the synthesis of 2'-deoxyfluoro adenine ribo- and arabino- nucleosides in moderate yield (24%)...
  15. doi request reprint Glucose-limited high cell density cultivations from small to pilot plant scale using an enzyme-controlled glucose delivery system
    Julia Glazyrina
    Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstraße 71 76, ACK 24, D 13355 Berlin, Germany
    N Biotechnol 29:235-42. 2012
    ..Because of the linear cell growth under glucose limitation it provides optimal and robust starting conditions for traditional external feed-based processes...
  16. ncbi request reprint Modelling of translation of human protein disulfide isomerase in Escherichia coli-A case study of gene optimisation
    Olli Niemitalo
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, Oulu, Finland
    J Biotechnol 120:11-24. 2005
    ..coli translational ribosome traffic based upon the stochastic Gillespie algorithm. The study indicates the applicability of such models in optimisation of recombinant protein sequences...
  17. pmc Physiology of resistant Deinococcus geothermalis bacterium aerobically cultivated in low-manganese medium
    Christina Liedert
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, Oulu, Finland
    J Bacteriol 194:1552-61. 2012
    ..geothermalis prefers to combat ROS using manganese-dependent enzymes, but when manganese is not available central carbon metabolism is used to produce ROS neutralizing metabolites at the expense of high utilization of carbon substrate...
  18. doi request reprint A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: impact of oscillations on Bacillus subtilis fed-batch cultivations
    Stefan Junne
    Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Berlin, Germany
    Biotechnol J 6:1009-17. 2011
    ..As a result, the reduced carbon flux entering the tricarboxylic acid cycle is not sufficient to support amino acid synthesis following the oxaloacetic acid branch point...
  19. doi request reprint Two-compartment method for determination of the oxygen transfer rate with electrochemical sensors based on sulfite oxidation
    Julia Glazyrina
    Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Berlin, Germany
    Biotechnol J 6:1003-8. 2011
    ..Under typical shaking conditions, k(L) a values in the standard baffled flasks reached values up to 220 h(-1) , whereas the k(L) a values of the Ultra Yield flasks were significantly higher (up to 422 h(-1) )...
  20. doi request reprint High-temperature cultivation and 5' mRNA optimization are key factors for the efficient overexpression of thermostable Deinococcus geothermalis purine nucleoside phosphorylase in Escherichia coli
    Kathleen Szeker
    Department of Biotechnology, Technische Universitat Berlin, Ackerstrasse 71 76, Berlin, Germany
    J Biotechnol 156:268-74. 2010
    ..We suggest that this method is especially suitable for improving the expression of proteins derived from thermophiles in E. coli...
  21. pmc High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor
    Julia Glazyrina
    Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstrasse 71 76, D 13355 Berlin, Germany
    Microb Cell Fact 9:42. 2010
    ..Here, we investigate the possibilities which both strategies offer in view of high cell density cultivation of E. coli and recombinant protein production...
  22. ncbi request reprint RNA-based sandwich hybridisation method for detection of lactic acid bacteria in brewery samples
    Sanna Huhtamella
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, Biocenter Oulu, University of Oulu, P O Box 4300, FI 90014 Oulu, Finland
    J Microbiol Methods 68:543-53. 2007
    ....
  23. pmc Norvaline is accumulated after a down-shift of oxygen in Escherichia coli W3110
    Jaakko Soini
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, P, O, Box 4300, FI 90014 Oulu, Finland
    Microb Cell Fact 7:30. 2008
    ..abstract:..
  24. doi request reprint Isolation and genotype-dependent, organ-specific expression analysis of a Rhodiola rosea cDNA encoding tyrosine decarboxylase
    Zsuzsanna György
    Department of Process and Environmental Engineering, University of Oulu, 90014 Oulu, Finland
    J Plant Physiol 166:1581-6. 2009
    ..The difference in the expression intensity suggests a decisive role for this enzyme in the salidroside biosynthesis...
  25. pmc Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures
    Kaisa Ukkonen
    Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, University of Oulu, Oulu, Finland
    Microb Cell Fact 12:73. 2013
    ..coli K-12 and E. coli BL21 when grown in different types of media and under different aeration conditions...
  26. pmc Enhanced plasmid production in miniaturized high-cell-density cultures of Escherichia coli supported with perfluorinated oxygen carrier
    Maciej Pilarek
    Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstrasse 71 76, ACK24, 13355, Berlin, Germany
    Bioprocess Biosyst Eng 36:1079-86. 2013
    ..Aside from the advantage of this method for the daily routine, we believe that it could also be applied to automated high-throughput processes. ..
  27. doi request reprint Use of slow glucose feeding as supporting carbon source in lactose autoinduction medium improves the robustness of protein expression at different aeration conditions
    Kaisa Ukkonen
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P O Box 4300, FI 90014 Oulu, Finland BioSilta Oy, P O Box 4300, FI 90014 Oulu, Finland Electronic address
    Protein Expr Purif 91:147-54. 2013
    ....
  28. doi request reprint Polyamine metabolism during exponential growth transition in Scots pine embryogenic cell culture
    Jaana Vuosku
    Department of Biology, University of Oulu, 90014 Oulu, Finland
    Tree Physiol 32:1274-87. 2012
    ..g., the effect of specific stressors on PA metabolism is addressed...
  29. pmc Quality control of inclusion bodies in Escherichia coli
    Britta Jürgen
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, FIN 90014 Oulu, Finland
    Microb Cell Fact 9:41. 2010
    ..IBs mostly contain only minor impurities and are relatively homogenous...
  30. doi request reprint Modification of buffered peptone water for improved recovery of heat-injured Salmonella Typhimurium
    Sanna Taskila
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P O Box 4300, Oulu FI 90014, Finland
    J Food Sci 76:M157-62. 2011
    ..The presented results can be used for the development of more efficient enrichment cultivation media for faster detection of food borne Salmonella...
  31. ncbi request reprint High-level production of human collagen prolyl 4-hydroxylase in Escherichia coli
    Antje Neubauer
    Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P O Box 5000, University of Oulu, FIN 90014 Oulu, Finland
    Matrix Biol 24:59-68. 2005
    ..The enzyme expressed in E. coli differed from those present in vivo and those produced in other hosts in that it lacked the N glycosylation of its alpha subunits, which may be advantageous in crystallization experiments...
  32. doi request reprint Two-dimensional proteome reference map for the radiation-resistant bacterium Deinococcus geothermalis
    Christina Liedert
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Linnanmaa, Oulu, Finland
    Proteomics 10:555-63. 2010
    ..A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis...
  33. ncbi request reprint Murine Wnt-1 with an internal c-myc tag recombinantly produced in Escherichia coli can induce intracellular signaling of the canonical Wnt pathway in eukaryotic cells
    Beatrix Fahnert
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P O Box 4300, FIN 90014 Oulu, Finland
    J Biol Chem 279:47520-7. 2004
    ..Thus, this approach could make recombinant murine Wnt-1 available as a good starting point for other Wnts needed, for example, for maintaining and differentiating stem cells, organ restoration therapy, and tissue engineering...
  34. pmc High cell density media for Escherichia coli are generally designed for aerobic cultivations - consequences for large-scale bioprocesses and shake flask cultures
    Jaakko Soini
    Bioprocess Engineering Laboratory, Dept, of Process and Environmental Engineering, University of Oulu, P, O, Box 4300, FIN 90014 Oulu, Finland
    Microb Cell Fact 7:26. 2008
    ..abstract:..
  35. ncbi request reprint Biotechnology - 12th European Congress
    Peter Neubauer
    University of Oulu, Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, Finland
    IDrugs 8:809-12. 2005
  36. ncbi request reprint 16S rRNA targeted sandwich hybridization method for direct quantification of mycobacteria in soils
    Timo Nieminen
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Finland
    J Microbiol Methods 67:44-55. 2006
    ..The 16S rRNA sandwich hybridization method described in this paper offers a culture independent solution for tracking environmental reservoirs of viable and potentially infectious mycobacteria...
  37. doi request reprint Octaketide-producing type III polyketide synthase from Hypericum perforatum is expressed in dark glands accumulating hypericins
    Katja Karppinen
    Department of Biology, University of Oulu, Finland
    FEBS J 275:4329-42. 2008
    ..Based on our results, HpPKS2 may have a role in the biosynthesis of hypericins in H. perforatum but some additional factors are possibly required for the production of emodin anthrone in vivo...
  38. pmc Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures
    Juozas Siurkus
    Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstr 71 76, D 13355 Berlin, Germany
    Microb Cell Fact 9:35. 2010
    ..Performing the screening already under fed-batch conditions would limit the amount of work and guarantee that the selected favoured conditions also work in the production scale...
  39. pmc Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase
    Ekaterina Osmekhina
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, P O Box 4300, FIN 90014 University of Oulu, Finland
    Microb Cell Fact 9:48. 2010
    ..Then the following holoenzyme detection is accomplished by a goat anti-rabbit IgG labeled with alkaline phosphatase which AP catalyzes the reaction of a substrate transformation with fluorescent signal generation...
  40. ncbi request reprint Inclusion bodies: formation and utilisation
    Beatrix Fahnert
    Biocenter Oulu, Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, University of Oulu, PO Box 4300, 90014 Oulu, Finland
    Adv Biochem Eng Biotechnol 89:93-142. 2004
    ..In addition, simple strategies have been developed to optimise the purification and renaturation of disulfide bond containing proteins making a fast transfer of such processes into the industrial production scale realistic...
  41. ncbi request reprint A role for bacteriophage T4 rI gene function in the control of phage development during pseudolysogeny and in slowly growing host cells
    Marcin Los
    Biocenter Oulu and Department of Process and Environmental Engineering, University of Oulu, Oulu, Finland
    Res Microbiol 154:547-52. 2003
    ..The rI gene, previously known to be indispensable for lysis inhibition, seems to play an important role in optimization of phage development in slowly growing cells as well as during establishment and maintenance of pseudolysogeny...
  42. ncbi request reprint Efficient lactic acid production from high salt containing dairy by-products by Lactobacillus salivarius ssp. salicinius with pre-treatment by proteolytic microorganisms
    Antti Vasala
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, FIN 90014 Oulu, Finland
    J Biotechnol 117:421-31. 2005
    ..The results show that fast and complete conversion of lactose to lactic acid can be obtained in dairy by-products without expensive additives...
  43. ncbi request reprint Functional role of the conserved active site proline of triosephosphate isomerase
    Marco G Casteleijn
    Department of Process and Environmental Engineering and Bioprocess Engineering Laboratory, University of Oulu, P O Box 3000, FIN 90014 Oulu, Finland
    Biochemistry 45:15483-94. 2006
    ..Apparently, the rotation of 90 degrees of the Gly211-Gly212 peptide plane of loop 7 plays a key role in this concerted movement...
  44. ncbi request reprint Sensitive genus-specific detection of Legionella by a 16S rRNA based sandwich hybridization assay
    Tarja Leskelä
    Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, PO Box 4300, FI 90014 University of Oulu, Finland
    J Microbiol Methods 62:167-79. 2005
    ..The results show that the developed SHA can be applied for estimation of the approximate number of Legionella cells based on the number of 16S rRNA molecules in a water sample...