E Neher

Summary

Country: Germany

Publications

  1. pmc What is Rate-Limiting during Sustained Synaptic Activity: Vesicle Supply or the Availability of Release Sites
    Erwin Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry Goettingen, Germany
    Front Synaptic Neurosci 2:144. 2010
  2. pmc Transients in global Ca2+ concentration induced by electrical activity in a giant nerve terminal
    Erwin Neher
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    J Physiol 591:3189-95. 2013
  3. doi request reprint Introduction: regulated exocytosis
    E Neher
    Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany
    Cell Calcium 52:196-8. 2012
  4. ncbi request reprint Complexin: does it deserve its name?
    Erwin Neher
    Max Planck Institute for Biophysical Chemistry, Department of Membrane Biophysics, 37077 Goettingen, Germany
    Neuron 68:803-6. 2010
  5. pmc Details of Ca2+ dynamics matter
    Erwin Neher
    Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Physiol 586:2031. 2008
  6. ncbi request reprint A comparison between exocytic control mechanisms in adrenal chromaffin cells and a glutamatergic synapse
    Erwin Neher
    Max Planck Institute for Biophysical Chemistry, 37077, Goettingen, Germany
    Pflugers Arch 453:261-8. 2006
  7. ncbi request reprint Usefulness and limitations of linear approximations to the understanding of Ca++ signals
    E Neher
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Cell Calcium 24:345-57. 1998
  8. doi request reprint Multiple roles of calcium ions in the regulation of neurotransmitter release
    Erwin Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Neuron 59:861-72. 2008
  9. ncbi request reprint Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates
    Erwin Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Neurosci Methods 130:143-57. 2003
  10. ncbi request reprint Estimating transmitter release rates from postsynaptic current fluctuations
    E Neher
    Max Planck Institute for Biophysical Chemistry, Department of Membrane Biophysics, D 37077 Gottingen, Germany
    J Neurosci 21:9638-54. 2001

Collaborators

Detail Information

Publications63

  1. pmc What is Rate-Limiting during Sustained Synaptic Activity: Vesicle Supply or the Availability of Release Sites
    Erwin Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry Goettingen, Germany
    Front Synaptic Neurosci 2:144. 2010
    ..Here it is argued that not the availability of vesicles is the limiting factor for recruitment, but rather the availability of specific sites to which vesicles can dock...
  2. pmc Transients in global Ca2+ concentration induced by electrical activity in a giant nerve terminal
    Erwin Neher
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    J Physiol 591:3189-95. 2013
    ....
  3. doi request reprint Introduction: regulated exocytosis
    E Neher
    Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany
    Cell Calcium 52:196-8. 2012
    ..The importance of using assays with high time resolution for separating these distinct roles is pointed out here...
  4. ncbi request reprint Complexin: does it deserve its name?
    Erwin Neher
    Max Planck Institute for Biophysical Chemistry, Department of Membrane Biophysics, 37077 Goettingen, Germany
    Neuron 68:803-6. 2010
    ..But it also provokes thoughts about alternative interpretations, which might result in a simpler model of complexin function...
  5. pmc Details of Ca2+ dynamics matter
    Erwin Neher
    Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Physiol 586:2031. 2008
  6. ncbi request reprint A comparison between exocytic control mechanisms in adrenal chromaffin cells and a glutamatergic synapse
    Erwin Neher
    Max Planck Institute for Biophysical Chemistry, 37077, Goettingen, Germany
    Pflugers Arch 453:261-8. 2006
    ..This enables us to clearly distinguish between consequences of changes in electrical signaling, from those regarding the process of vesicle recruitment or the process of exocytosis...
  7. ncbi request reprint Usefulness and limitations of linear approximations to the understanding of Ca++ signals
    E Neher
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Cell Calcium 24:345-57. 1998
    ....
  8. doi request reprint Multiple roles of calcium ions in the regulation of neurotransmitter release
    Erwin Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Neuron 59:861-72. 2008
    ..Such coupling is essential for rendering vesicles sensitive to short [Ca(2+)] transients, generated during action potentials...
  9. ncbi request reprint Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates
    Erwin Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Neurosci Methods 130:143-57. 2003
    ..Several ways to handle non-stationarities are described. Examples are provided for the Calyx of Held, a glutamatergic synapse, in which both the pre- and the postsynaptic compartments can be voltage-clamped...
  10. ncbi request reprint Estimating transmitter release rates from postsynaptic current fluctuations
    E Neher
    Max Planck Institute for Biophysical Chemistry, Department of Membrane Biophysics, D 37077 Gottingen, Germany
    J Neurosci 21:9638-54. 2001
    ..MiniatureEPSC (mEPSC) size is estimated to be similar to the value of spontaneously occurring mEPSC under this condition...
  11. ncbi request reprint Quantitative relationship between transmitter release and calcium current at the calyx of held synapse
    T Sakaba
    Max-Planck-Institute for Biophysical Chemistry, Department of Membrane Biophysics, D-37077, , Germany
    J Neurosci 21:462-76. 2001
    ..Heterogeneous release probabilities are expected to support the maintenance of synaptic transmission during high-frequency stimulation...
  12. pmc Kinetics of the secretory response in bovine chromaffin cells following flash photolysis of caged Ca2+
    C Heinemann
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 67:2546-57. 1994
    ..The predicted Ca2+ dissociation constant (Kd) of a single Ca(2+)-binding site is between 7 and 21 microM. At [Ca2+]i > 30 microM clear indications of a fast endocytotic process complicate the analysis of the secretory response...
  13. pmc Two-dimensional determination of the cellular Ca2+ binding in bovine chromaffin cells
    M Naraghi
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Biophys J 75:1635-47. 1998
    ....
  14. ncbi request reprint Released fraction and total size of a pool of immediately available transmitter quanta at a calyx synapse
    R Schneggenburger
    Max Planck Institut fur biophysikalische Chemie, Abteilung Membranbiophysik, Gottingen, Germany
    Neuron 23:399-409. 1999
    ..The results reveal a surprisingly large pool of functionally available vesicles, of which a fraction of about 0.2 is released by a single presynaptic action potential under physiological conditions...
  15. ncbi request reprint The readily releasable pool of vesicles in chromaffin cells is replenished in a temperature-dependent manner and transiently overfills at 37 degrees C
    V Dinkelacker
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    J Neurosci 20:8377-83. 2000
    ..We conclude that vesicle maturation is markedly accelerated at physiological temperature, thus allowing for a rapid adaptation of the pool size to the relatively short-lived Ca(2+) transient...
  16. ncbi request reprint Intracellular calcium dependence of transmitter release rates at a fast central synapse
    R Schneggenburger
    Abteilung Membranbiophysik, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Nature 406:889-93. 2000
    ..This non-saturation, and the high intracellular calcium cooperativity in triggering vesicle fusion, make fast synaptic transmission very sensitive to modulation by changes in local [Ca2+]i...
  17. pmc Preferential potentiation of fast-releasing synaptic vesicles by cAMP at the calyx of Held
    T Sakaba
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg, , Germany
    Proc Natl Acad Sci U S A 98:331-6. 2001
    ..cAMP increases the apparent Ca2+ sensitivity for secretion, but this increase does not reflect an increase in release probability necessarily but rather an increase in the number of highly sensitive vesicles...
  18. ncbi request reprint Combining deconvolution and noise analysis for the estimation of transmitter release rates at the calyx of held
    E Neher
    Max-Planck-Institute for Biophysical Chemistry, Department of Membrane Biophysics, D-37077, , Germany
    J Neurosci 21:444-61. 2001
    ..Moreover, the method presented should provide a convenient way to study the kinetics of transmitter release at other synapses...
  19. ncbi request reprint Protein kinase C-dependent phosphorylation of synaptosome-associated protein of 25 kDa at Ser187 potentiates vesicle recruitment
    Gabor Nagy
    Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Neurosci 22:9278-86. 2002
    ..Thus the C terminus of SNAP-25 plays a role for both fast exocytosis triggering and vesicle recruitment, and the latter process is regulated by PKC-dependent phosphorylation...
  20. ncbi request reprint Multiple calcium-dependent processes related to secretion in bovine chromaffin cells
    E Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Neuron 10:21-30. 1993
    ..Both secretion and movement of vesicles between pools appear to be [Ca2+]i sensitive. Later [Ca2+]i steps sometimes evoked a rapid capacitance decrease, indicating a fast, [Ca2+]i-dependent phase of endocytosis...
  21. ncbi request reprint Mechanisms underlying phasic and sustained secretion in chromaffin cells from mouse adrenal slices
    T Voets
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Federal Republic of Germany
    Neuron 23:607-15. 1999
    ..An intermediate pool in dynamic equilibrium with the RRP ensures rapid recruitment of release-ready vesicles after RRP depletion. Our results are discussed in terms of a refined model for secretion in chromaffin cells...
  22. ncbi request reprint Swelling-induced catecholamine secretion recorded from single chromaffin cells
    T Moser
    Abteilung Membranbiophysik, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Pflugers Arch 431:196-203. 1995
    ..Therefore, cell volume changes may modulate the secretory activity...
  23. ncbi request reprint Mechanisms determining the time course of secretion in neuroendocrine cells
    R H Chow
    Max Planck Institute for Biophysical Chemistry, Department of Membrane Biophysics, Goettingen, Germany
    Neuron 16:369-76. 1996
    ..This suggests that most of the delay is due to diffusion of Ca2+ between channels and release sites, implying that Ca2+ channels and secretory vesicles are not colocalized in chromaffin cells, in contrast to presynaptic active zones...
  24. ncbi request reprint R-Type Ca2+ channels are coupled to the rapid component of secretion in mouse adrenal slice chromaffin cells
    A Albillos
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany
    J Neurosci 20:8323-30. 2000
    ..Our results indicate that R-type Ca(2+) channels are in close proximity with the exocytotic machinery to rapidly regulate the secretory process...
  25. ncbi request reprint The coupling between synaptic vesicles and Ca2+ channels determines fast neurotransmitter release
    Kristian Wadel
    Research Group Biophysics of Synaptic Transmission, Max Planck Institute for Biophysical Chemistry, Gottingen, 37077, Germany
    Neuron 53:563-75. 2007
    ..We conclude that recruitment of synaptic vesicles to sites where Ca2+ channels cluster, rather than fusion competence, is a limiting step for rapid neurotransmitter release in response to presynaptic action potentials...
  26. ncbi request reprint Calcium dependence of exocytosis and endocytosis at the cochlear inner hair cell afferent synapse
    D Beutner
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37077, Gottingen, Germany
    Neuron 29:681-90. 2001
    ..These properties provide for rapid and sustained transmitter release from this large presynaptic terminal...
  27. pmc Release kinetics, quantal parameters and their modulation during short-term depression at a developing synapse in the rat CNS
    Holger Taschenberger
    Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Physiol 568:513-37. 2005
    ..Therefore, a multitude of mechanisms seems to converge on the generation of fast, temporally precise and reliable high-frequency transmission at the mature calyx of Held synapse...
  28. ncbi request reprint Quantitative analysis of calcium-dependent vesicle recruitment and its functional role at the calyx of Held synapse
    Nobutake Hosoi
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Neurosci 27:14286-98. 2007
    ..We could also demonstrate an approximate sixfold increase in release probability (facilitation) during the initial 5-15 AP-like stimuli of such trains in our experimental condition, regardless of EPSC depression...
  29. pmc Kinetics of both synchronous and asynchronous quantal release during trains of action potential-evoked EPSCs at the rat calyx of Held
    V Scheuss
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    J Physiol 585:361-81. 2007
    ..The short duration of asynchronous release at the calyx of Held synapse may aid in generating brief postsynaptic depolarizations, avoiding temporal summation and preserving action potential timing during high frequency bursts...
  30. ncbi request reprint Calmodulin mediates rapid recruitment of fast-releasing synaptic vesicles at a calyx-type synapse
    T Sakaba
    Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, D-37077, Goettingen, Germany
    Neuron 32:1119-31. 2001
    ..We propose that calmodulin-dependent refilling supports recovery from synaptic depression during high-frequency trains in concert with rapid recovery of the slowly releasing vesicles...
  31. ncbi request reprint Direct modulation of synaptic vesicle priming by GABA(B) receptor activation at a glutamatergic synapse
    Takeshi Sakaba
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany
    Nature 424:775-8. 2003
    ....
  32. ncbi request reprint Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25
    Gabor Nagy
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Neuron 41:417-29. 2004
    ..Overexpression of the SNAP-25b isoform increased the size of both primed vesicle pools by a factor of two, and mutations in the conserved Thr-138 site had similar effects as in the a isoform...
  33. pmc The timing of phasic transmitter release is Ca2+-dependent and lacks a direct influence of presynaptic membrane potential
    Felix Felmy
    AG Synaptische Dynamik und Modulation and Abteilung Membranbiophysik, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 100:15200-5. 2003
    ....
  34. pmc Intracellular calcium dependence of large dense-core vesicle exocytosis in the absence of synaptotagmin I
    T Voets
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 98:11680-5. 2001
    ..Alternatively, synaptotagmin I could function as calcium sensor for the readily releasable pool, leading to the destabilization of the pool in its absence...
  35. ncbi request reprint A two-step model of secretion control in neuroendocrine cells
    C Heinemann
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Pflugers Arch 424:105-12. 1993
    ..Given these functional dependences a variety of results from the literature can be simulated. In particular, the model predicts the occurrence of secretory depression and augmentation under appropriate conditions...
  36. ncbi request reprint Involvement of actin polymerization in vesicle recruitment at the calyx of Held synapse
    Takeshi Sakaba
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, D 37077, Germany
    J Neurosci 23:837-46. 2003
    ..The results suggest that intact actin polymerization is involved in vesicle recruitment...
  37. ncbi request reprint Protein kinase C enhances exocytosis from chromaffin cells by increasing the size of the readily releasable pool of secretory granules
    K D Gillis
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Federal Republic of Germany
    Neuron 16:1209-20. 1996
    ..Thus, PKC appears to affect a late step in secretion but not the Ca2+ sensitivity of the final step...
  38. pmc Highly nonlinear photodamage in two-photon fluorescence microscopy
    A Hopt
    , Department of Membrane Biophysics, D-37077 Goettingen, Germany
    Biophys J 80:2029-36. 2001
    ..5) than that of the two-photon signal (mu approximately 2)...
  39. ncbi request reprint Two dye two wavelength excitation calcium imaging: results from bovine adrenal chromaffin cells
    M Oheim
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Cell Calcium 24:71-84. 1998
    ..Comparing fluorescence emission from in vitro dye solutions with in vivo values, we could estimate the accessible volume fraction and amount of Ca(2+)-insensitive dye...
  40. ncbi request reprint Probing the intracellular calcium sensitivity of transmitter release during synaptic facilitation
    Felix Felmy
    Abteilung Membranbiophysik and AG Synaptische Dynamik und Modulation, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Neuron 37:801-11. 2003
    ..However, a small degree of supralinearity in the summation of intracellular Ca(2+) signals, which might be caused by saturation of cytosolic Ca(2+) buffer(s), is sufficient to explain facilitation at this CNS synapse...
  41. pmc Volume-sensitive chloride conductance in bovine chromaffin cell membrane
    P Doroshenko
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    J Physiol 449:197-218. 1992
    ..abstract truncated at 400 words)..
  42. ncbi request reprint Pertussis-toxin-sensitive inhibition by (-) baclofen of Ca signals in bovine chromaffin cells
    P Doroshenko
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Federal Republic of Germany
    Pflugers Arch 419:444-9. 1991
    ..It is concluded that bovine chromaffin cells have functional GABAb receptors the activation of which, mediated by a PTX-sensitive GTP-binding protein, inhibits the evoked increase in cytosolic free Ca2+.(ABSTRACT TRUNCATED AT 250 WORDS)..
  43. doi request reprint Synaptotagmin has an essential function in synaptic vesicle positioning for synchronous release in addition to its role as a calcium sensor
    Samuel M Young
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Goettingen, Germany
    Neuron 63:482-96. 2009
    ....
  44. doi request reprint Application of an Epac activator enhances neurotransmitter release at excitatory central synapses
    Isabella Gekel
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Neurosci 28:7991-8002. 2008
    ..Because the PDBu response was abolished after downregulation of intracellular PKC activity, we conclude that ESCA(1)-induced Epac activation leads to presynaptic changes involving Epac-to-PKC signaling...
  45. ncbi request reprint Distinct kinetic changes in neurotransmitter release after SNARE protein cleavage
    Takeshi Sakaba
    Department of Neurobiology and Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany
    Science 309:491-4. 2005
    ..When stimulating release by calcium influx through channels, a difference between BoNT/C1 and TeNT emerged, which suggests that cleavage of synaptobrevin modifies the coupling between channels and release-competent vesicles...
  46. ncbi request reprint Presynaptic calcium and control of vesicle fusion
    Ralf Schneggenburger
    AG Synaptische Dynamik and Modulation and Abt Membranbiophysik, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Curr Opin Neurobiol 15:266-74. 2005
    ....
  47. pmc The SNARE protein SNAP-25 is linked to fast calcium triggering of exocytosis
    Jakob B Sørensen
    Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 99:1627-32. 2002
    ..We conclude that the SNARE complex is linked directly to calcium triggering of exocytosis, most likely in a complex with auxiliary proteins...
  48. ncbi request reprint Vesicle pools and short-term synaptic depression: lessons from a large synapse
    Ralf Schneggenburger
    Max Planck Institut fur biophysikalische Chemie, Abteilung Membranbiophysik, Am Fassberg, D 37077 Gottingen, Germany
    Trends Neurosci 25:206-12. 2002
    ..By influencing the synaptic output during repetitive activity, vesicle pool dynamics are expected to modulate information processing in neuronal networks of the CNS...
  49. ncbi request reprint Estimation of quantal parameters at the calyx of Held synapse
    Takeshi Sakaba
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, D37077 Gottingen, Germany
    Neurosci Res 44:343-56. 2002
    ..These factors should always be taken into account for fully understanding the mechanisms of synaptic transmission and plasticity...
  50. ncbi request reprint Differential control of the releasable vesicle pools by SNAP-25 splice variants and SNAP-23
    Jakob B Sørensen
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Cell 114:75-86. 2003
    ..We conclude that three alternative SNARE components support exocytosis, but they differ in their ability to stabilize vesicles in the primed state...
  51. pmc Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2+
    Jakob B Sørensen
    Max Planck Institut fur biophysikalische Chemie, Abteilung Membranbiophysik, Am Fassberg 11, D 37077 Gottingen, Germany
    J Gen Physiol 122:265-76. 2003
    ..Single amperometric fusion events were unchanged. We conclude that Ca2+-dependent phospholipid binding to synaptotagmin 1 mirrors the intracellular Ca2+ dependence of exocytosis...
  52. ncbi request reprint Munc18-1 promotes large dense-core vesicle docking
    T Voets
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Gottingen, Germany
    Neuron 31:581-91. 2001
    ..We conclude that Munc18-1 functions upstream of SNARE complex formation and promotes LDCV docking...
  53. doi request reprint Fast vesicle fusion in living cells requires at least three SNARE complexes
    Ralf Mohrmann
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Science 330:502-5. 2010
    ..Heterogeneity in SNARE-complex number may explain heterogeneity in vesicular release probability...
  54. ncbi request reprint Plasmalemmal phosphatidylinositol-4,5-bisphosphate level regulates the releasable vesicle pool size in chromaffin cells
    Ira Milosevic
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    J Neurosci 25:2557-65. 2005
    ....
  55. pmc Analysis of FRET signals in the presence of free donors and acceptors
    Jakub Wlodarczyk
    Deutsche Forschungsgemeinschaft Research Center for the Molecular Physiology of the Brain, Gottingen, Germany
    Biophys J 94:986-1000. 2008
    ..We call the method lux-FRET, since it relies on linear unmixing of spectral components...
  56. ncbi request reprint The most important recent advances in synapse research from my point of view--and what remains to be done
    Michael Frotscher
    Cell Tissue Res 326:203-4. 2006
  57. ncbi request reprint Emerging roles of presynaptic proteins in Ca++-triggered exocytosis
    Jens Rettig
    Department of Physiology, Saarland University, Homburg, 66421 Germany
    Science 298:781-5. 2002
    ..We discuss the molecular players that have been assigned a specific role in a particular step of this process and give a brief outlook on what these insights might tell us about mechanisms of short-term plasticity at classical synapses...
  58. ncbi request reprint Separation of presynaptic and postsynaptic contributions to depression by covariance analysis of successive EPSCs at the calyx of held synapse
    Volker Scheuss
    Max Planck Institute für biophysikalische Chemie, Abteilung Membranbiophysik 140, D 37077 Gottingen, Germany
    J Neurosci 22:728-39. 2002
    ..The number of release sites estimated by covariance analysis was approximately 2000 and significantly larger than estimates from variance-mean parabolas...
  59. pmc Heterogeneous presynaptic release probabilities: functional relevance for short-term plasticity
    Julia Trommershäuser
    Institute for Theoretical Physics, Georg August University of Gottingen, Germany
    Biophys J 84:1563-79. 2003
    ..We conclude that synaptic transmission cannot be accurately described unless heterogeneity of synaptic release probability is taken into account...
  60. ncbi request reprint Short-term plasticity turns plastic. Focus on "synaptic transmission at the calyx of held under in vivo-like activity levels"
    Erwin Neher
    J Neurophysiol 98:577-8. 2007
  61. pmc Specificity emerges in the dissection of diacylglycerol- and protein kinase C-mediated signalling pathways
    Nils Brose
    Max Planck Institut fur experimentelle Medizin, Hermann Rein Strasse 3, D 37075 Gottingen, Germany
    Proc Natl Acad Sci U S A 99:16522-3. 2002
  62. doi request reprint Stimulation- and palmitoylation-dependent changes in oligomeric conformation of serotonin 5-HT1A receptors
    Fritz Kobe
    DFG Research Center for the Molecular Physiology of the Brain CMPB, University of Gottingen, Germany
    Biochim Biophys Acta 1783:1503-16. 2008
    ..In contrast, the agonist-mediated decrease of FRET signal was completely abolished in oligomers composed by non-palmitoylated receptor mutants, demonstrating the importance of palmitoylation in modulation of the structure of oligomers...
  63. ncbi request reprint Applying spectral fingerprinting to the analysis of FRET images
    Richard A Neher
    LMU Munchen, Department für Physik, 80333 München, Germany
    Microsc Res Tech 64:185-95. 2004
    ..We compare the expected resolution of traditional FRET measures with that of optimized spectral fingerprinting and analyze the resolution of a method for FRET measurements that combines spectral with fluorescence lifetime information...