Ingo Morano

Summary

Country: Germany

Publications

  1. ncbi Tuning smooth muscle contraction by molecular motors
    Ingo Morano
    Max Delbrück Centrum for Molecular Medicine and Humboldt University, Johannes Müller Institute for Physiology, Berlin, Germany
    J Mol Med (Berl) 81:481-7. 2003
  2. ncbi Tuning the human heart molecular motors by myosin light chains
    I Morano
    Max Delbruck Center for Molecular Medicine, Berlin Buch, Germany
    J Mol Med (Berl) 77:544-55. 1999
  3. ncbi Human essential myosin light chain isoforms revealed distinct myosin binding, sarcomeric sorting, and inotropic activity
    Daria Petzhold
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Cardiovasc Res 90:513-20. 2011
  4. ncbi The carboxyl-terminal ahnak domain induces actin bundling and stabilizes muscle contraction
    Hannelore Haase
    Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
    FASEB J 18:839-41. 2004
  5. ncbi Human atrial myosin light chain 1 expression attenuates heart failure
    Ahmed Ihab Abdelaziz
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125 Berlin, Germany
    Adv Exp Med Biol 565:283-92; discussion 92, 405-15. 2005
  6. ncbi Minigenes encoding N-terminal domains of human cardiac myosin light chain-1 improve heart function of transgenic rats
    Hannelore Haase
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    FASEB J 20:865-73. 2006
  7. ncbi Mutations of ventricular essential myosin light chain disturb myosin binding and sarcomeric sorting
    Janine Lossie
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, Berlin 13125, Germany
    Cardiovasc Res 93:390-6. 2012
  8. ncbi Modulation of muscle contraction by a cell-permeable peptide
    Gisela Tunnemann
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125, Berlin, Germany
    J Mol Med (Berl) 85:1405-12. 2007
  9. ncbi Ahnak1 is a tuneable modulator of cardiac Ca(v)1.2 calcium channel activity
    Ines Pankonien
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125, Berlin, Germany
    J Muscle Res Cell Motil 32:281-90. 2011
  10. ncbi Ahnak1 abnormally localizes in muscular dystrophies and contributes to muscle vesicle release
    Ute Zacharias
    Muscle Research Unit, Experimental and Clinical Research Center, a joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, Berlin, Germany
    J Muscle Res Cell Motil 32:271-80. 2011

Collaborators

Detail Information

Publications42

  1. ncbi Tuning smooth muscle contraction by molecular motors
    Ingo Morano
    Max Delbrück Centrum for Molecular Medicine and Humboldt University, Johannes Müller Institute for Physiology, Berlin, Germany
    J Mol Med (Berl) 81:481-7. 2003
    ....
  2. ncbi Tuning the human heart molecular motors by myosin light chains
    I Morano
    Max Delbruck Center for Molecular Medicine, Berlin Buch, Germany
    J Mol Med (Berl) 77:544-55. 1999
    ..Mutations in all ventricular myosin subunits have been found in patients with hypertrophic cardiomyopathy...
  3. ncbi Human essential myosin light chain isoforms revealed distinct myosin binding, sarcomeric sorting, and inotropic activity
    Daria Petzhold
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Cardiovasc Res 90:513-20. 2011
    ....
  4. ncbi The carboxyl-terminal ahnak domain induces actin bundling and stabilizes muscle contraction
    Hannelore Haase
    Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
    FASEB J 18:839-41. 2004
    ..Our results suggest that the carboxyl-terminal ahnak domain exerts a stabilizing effect on muscle contractility via its interaction with actin of thin filaments...
  5. ncbi Human atrial myosin light chain 1 expression attenuates heart failure
    Ahmed Ihab Abdelaziz
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125 Berlin, Germany
    Adv Exp Med Biol 565:283-92; discussion 92, 405-15. 2005
    ..These results demonstrate that the expression of hALC-1 could have a beneficial effect on the overloaded hypertrophied heart...
  6. ncbi Minigenes encoding N-terminal domains of human cardiac myosin light chain-1 improve heart function of transgenic rats
    Hannelore Haase
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    FASEB J 20:865-73. 2006
    ..The positive inotropic effect of MLC-1 peptides occurred in the absence of a hypertrophic response. Thus, expression of N-terminal domains of MLC-1 represent a valuable tool for the treatment of the failing heart...
  7. ncbi Mutations of ventricular essential myosin light chain disturb myosin binding and sarcomeric sorting
    Janine Lossie
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, Berlin 13125, Germany
    Cardiovasc Res 93:390-6. 2012
    ....
  8. ncbi Modulation of muscle contraction by a cell-permeable peptide
    Gisela Tunnemann
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125, Berlin, Germany
    J Mol Med (Berl) 85:1405-12. 2007
    ....
  9. ncbi Ahnak1 is a tuneable modulator of cardiac Ca(v)1.2 calcium channel activity
    Ines Pankonien
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125, Berlin, Germany
    J Muscle Res Cell Motil 32:281-90. 2011
    ..But, tuning ahnak1/Cavβ(2) interaction provides a tool for modulating the beta-adrenergic response of I (CaL)...
  10. ncbi Ahnak1 abnormally localizes in muscular dystrophies and contributes to muscle vesicle release
    Ute Zacharias
    Muscle Research Unit, Experimental and Clinical Research Center, a joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, Berlin, Germany
    J Muscle Res Cell Motil 32:271-80. 2011
    ..Taken together, our results define ahnak1 in muscle connective tissue as a novel feature of two genetically distinct muscular dystrophies that might contribute to disease pathology...
  11. ncbi Potential relevance of alpha(1)-adrenergic receptor autoantibodies in refractory hypertension
    Katrin Wenzel
    Medical Faculty of the Charite, Franz Volhard Clinic and HELIOS Klinikum Berlin, Experimental and Clinical Research Center, Berlin, Germany
    PLoS ONE 3:e3742. 2008
    ..We implied earlier that alpha(1)-AAB might have a mechanistic role and could represent a therapeutic target...
  12. ncbi Constriction velocities of renal afferent and efferent arterioles of mice are not related to SMB expression
    Andreas Patzak
    University Medicine Charité, Johannes Müller Institute for Physiology, Berlin, Germany
    Kidney Int 68:2726-34. 2005
    ....
  13. ncbi Auto-inhibitory effects of an IQ motif on protein structure and function
    Daria Petzhold
    Max Delbruck Center for Molecular Medicine, Department of Molecular Muscle Physiology, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Biochem Biophys Res Commun 396:939-43. 2010
    ..The collapsed structure of myosin with denuded IQ2 could explain the auto-inhibitory effects of IQ2 on enzymatic activity of myosin...
  14. ncbi Ahnak1 interaction is affected by phosphorylation of Ser-296 on Cavβ₂
    Ines Pankonien
    Max Delbruck Center for Molecular Medicine, Department of Molecular Muscle Physiology, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Biochem Biophys Res Commun 421:184-9. 2012
    ..We propose that this phosphorylation event is one mechanism underlying ahnak1's modulator function on Cav1.2 channel activity...
  15. ncbi A myomesin mutation associated with hypertrophic cardiomyopathy deteriorates dimerisation properties
    Romy Siegert
    Max Delbruck Center for Molecular Medicine, Department of Molecular Muscle Physiology, Robert Rossle Str 10, 13125 Berlin, Germany
    Biochem Biophys Res Commun 405:473-9. 2011
    ..In conclusion, the V1490I mutation associated with HCM lead to myomesin proteins with abnormal functional properties which affect dimerisation properties of myomesin domain 13. These effects may contribute to the pathogenesis of HCM...
  16. ncbi Distinct contractile systems for electromechanical and pharmacomechanical coupling in smooth muscle
    Valeria Lamounier-Zepter
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Adv Exp Med Biol 538:417-25; discussion 425-6. 2003
    ..ii) Pharmacomechanical coupling recruits part of the NM-MyHC system rather than SM-MyHC...
  17. ncbi Functional characterization of the human atrial essential myosin light chain (hALC-1) in a transgenic rat model
    Ahmed Ihab Abdelaziz
    Max Delbrück Center for Molecular Medicine MDC, Molecular Muscle Physiology, Robert Rossle Strasse 10, 13125 Berlin, Germany
    J Mol Med (Berl) 82:265-74. 2004
    ..2 to 1,364 +/- 137.4 mmHg/s in the relaxation rate in the WKY and the TGR groups respectively. Characterizing the functional effects of hALC-1 at the whole organ level represents a step towards gene therapy of heart failure...
  18. ncbi AHNAK1 and AHNAK2 are costameric proteins: AHNAK1 affects transverse skeletal muscle fiber stiffness
    Andreas Marg
    Max Delbruck Centrum fur Molekulare Medizin, D 13092 Berlin, Germany
    Biochem Biophys Res Commun 401:143-8. 2010
    ..Using atomic force microscopy (AFM), we observed a significantly higher transverse stiffness of AHNAK1⁻/⁻ fibers. These findings suggest novel functions of AHNAK proteins in skeletal muscle...
  19. ncbi Ahnak is critical for cardiac Ca(V)1.2 calcium channel function and its beta-adrenergic regulation
    Hannelore Haase
    Max Delbrück Center for Molecular Medicine MDC, D 13092 Berlin, Germany
    FASEB J 19:1969-77. 2005
    ..This genetic ahnak variant might cause individual differences in I(CaL) regulation upon physiological challenges or therapeutic interventions...
  20. ncbi Phosphorylation of phospholamban correlates with relaxation of coronary artery induced by nitric oxide, adenosine, and prostacyclin in the pig
    P Karczewski
    Max Delbruck Center for Molecular Medicine, Berlin Buch, Germany
    J Cell Biochem 70:49-59. 1998
    ....
  21. ncbi Spinophilin is required for normal morphology, Ca(2+) homeostasis and contraction but dispensable for β-adrenergic stimulation of adult cardiomyocytes
    Daria Petzhold
    Max Delbrück Center for Molecular Medicine MDC, Robert Rossle Str 10, 13092, Berlin, Germany
    J Muscle Res Cell Motil 32:243-8. 2011
    ..Our data suggest that spinophilin is an upstream regulator required for normal growth and excitation-contraction coupling, but is dispensable for β-adrenergic stimulation of adult cardiomyocytes...
  22. ncbi Connective tissue growth factor overexpression in cardiomyocytes promotes cardiac hypertrophy and protection against pressure overload
    Anna N Panek
    Department of Cardiovascular and Metabolic Disease Research, Max Delbruck Center for Molecular Medicine, Berlin, Germany
    PLoS ONE 4:e6743. 2009
    ..Moreover, it is involved in hypertrophy induction and cellular remodeling depending on the cardiac stress stimulus. Our new transgenic animals are valuable models for reconsideration of CTGF's profibrotic function in the heart...
  23. ncbi Backbone rigidity and static presentation of guanidinium groups increases cellular uptake of arginine-rich cell-penetrating peptides
    Gisela Lättig-Tünnemann
    Max Delbrueck Center for Molecular Medicine, Berlin, Germany
    Nat Commun 2:453. 2011
    ..We propose that guanidinium groups are forced into maximally distant positions by cyclization. This orientation increases membrane contacts leading to enhanced cell penetration...
  24. ncbi Androgen receptor independent cardiovascular action of the antiandrogen flutamide
    Radu Iliescu
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13125 Berlin Buch, Germany
    J Mol Med (Berl) 81:420-7. 2003
    ..This response may contribute to the observed antihypertensive actions of flutamide...
  25. ncbi Expression of smooth muscle MyHC B in blood vessels of hypertrophied heart in experimentally hypertensive rats
    Katharina Wetzel
    Max Delbruck Center for Molecular Medicine, 13122 Berlin Buch, Germany
    Am J Physiol Regul Integr Comp Physiol 284:R607-10. 2003
    ..These observations suggest that the downregulation of SMB in spontaneously hypertensive rats is of genetic origin rather than an adaptive response to chronically enhanced blood pressure and cardiac hypertrophy...
  26. ncbi Different actin affinities of human cardiac essential myosin light chain isoforms
    I Morano
    Max Delbruck Center for Molecular Medicine, Berlin Buch, Germany
    FEBS Lett 408:71-4. 1997
    ..Weak binding of ALC-1 to actin could explain the higher cycling kinetics of cross-bridges with ALC-1 compared to those with VLC-1...
  27. ncbi alpha-Tropomyosin mutations Asp(175)Asn and Glu(180)Gly affect cardiac function in transgenic rats in different ways
    Dirk Wernicke
    Max Delbruck Center for Molecular Medicine, Robert Roessle Str 10, Berlin 13092, Germany
    Am J Physiol Regul Integr Comp Physiol 287:R685-95. 2004
    ....
  28. ncbi The carboxyl-terminal region of ahnak provides a link between cardiac L-type Ca2+ channels and the actin-based cytoskeleton
    Annette Hohaus
    Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
    FASEB J 16:1205-16. 2002
    ..These results suggest that ahnak provides a structural basis for the subsarcolemmal cytoarchitecture and confers the regulatory role of the actin-based cytoskeleton to the L-type Ca2+ channel...
  29. ncbi Hypoxia induces dilated cardiomyopathy in the chick embryo: mechanism, intervention, and long-term consequences
    Andrei Tintu
    Laboratory for Angiogenesis and Cardiovascular Pathology, Max Delbrueck Center for Molecular Medicine, Berlin, Germany
    PLoS ONE 4:e5155. 2009
    ..We have previously shown that chronic hypoxia alters cardiovascular development in chick embryos. The aim of this study was to further characterize cardiac disease in hypoxic chick embryos...
  30. ncbi The human beta-myosin heavy chain gene: sequence diversity and functional characteristics of the protein
    B Wendel
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    J Cell Biochem 79:566-75. 2000
    ..Analysis of the kinetic behaviour of beta-MHC in the intact contractile structure of normal individuals and HCM patients revealed apparent rate constants of tension development ranging between 1.58 s(-1) and 1.48 s(-1)...
  31. ncbi Analysis of the energetic state of heart cells after adenovirus-mediated expression of hALC-1
    Udo B Zacharzowsky
    Max Delbrück Center for Molecular Medicine MDC, 13122 Berlin, Germany
    J Cell Biochem 86:422-31. 2002
    ..These data suggest that first: adenoviral vectors could be used as a safe and effective tool for gene transfer to cardiomyocytes, and second: that a positive inotropic effect of hALC-1 is not associated with enhanced oxygen consumption...
  32. ncbi The L-type Ca2+-channel subunits alpha1C and beta2 are not downregulated in atrial myocardium of patients with chronic atrial fibrillation
    Ulrich Schotten
    Department of Physiology, Maastricht University, PO Box 616 6200 MD, The, Maastricht, Netherlands
    J Mol Cell Cardiol 35:437-43. 2003
    ..We investigated whether the expression of L-type Ca2+-channel subunits was reduced in atrial myocardium of AF patients...
  33. ncbi Nonmuscle Myosin motor of smooth muscle
    Mia Löfgren
    Department of Physiological Sciences, Medical Faculty, Lund University, SE 221 84 Lund, Sweden
    J Gen Physiol 121:301-10. 2003
    ..High ADP binding and low phosphate dependence of nonmuscle myosin would influence both velocity of actin translocation and force generation to promote slow motility and economical force maintenance of the cell...
  34. ncbi Regulation of the human atrial myosin light chain 1 promoter by Ca2+-calmodulin-dependent signaling pathways
    Christiane Woischwill
    , Berlin-Buch, Germany
    FASEB J 19:503-11. 2005
    ..Thus, our results demonstrate a dominant role of Ca2+-calmodulin-dependent signaling pathways in the regulation of human ALC-1 expression...
  35. ncbi Smooth, slow and smart muscle motors
    Anders Arner
    Department of Physiological Sciences, Medical Faculty, Lund University, BMC F11, Tornavagen 10, SE 221 84 Lund, Sweden
    J Muscle Res Cell Motil 24:165-73. 2003
    ..Non-muscle myosin has been shown to have a contractile function in some smooth muscle tissues and recent data on the kinetic properties of non-muscle myosin filaments in smooth muscle tissue are described...
  36. ncbi Calcium current in rat cardiomyocytes is modulated by the carboxyl-terminal ahnak domain
    Julio Alvarez
    Physiopathologie Cardiovasculaire, INSERM U 390, CHU Arnaud de Villeneuve, F 34295 Montpellier cedex 5, France
    J Biol Chem 279:12456-61. 2004
    ....
  37. ncbi Human adipocytes attenuate cardiomyocyte contraction: characterization of an adipocyte-derived negative inotropic activity
    Valeria Lamounier-Zepter
    Medical Clinic III, University of Dresden, Fetscherstr 74, Dresden 01307, Germany
    FASEB J 20:1653-9. 2006
    ..In summary, adipose tissue exerts highly potent activity with an acute depressant effect directly on cardiomyocytes, which may well contribute to increased heart failure risk in overweight patients...
  38. ncbi Regional expression of L-type calcium channel subunits during cardiac development
    Lourdes Acosta
    Department of Experimental Biology, University of Jaen, Jaen, Spain
    Dev Dyn 230:131-6. 2004
    ..These findings suggest that beta subunits might play a major role in conferring calcium handling heterogeneity within the developing embryonic myocardium, while alpha1C subunits might contribute just transiently...
  39. ncbi Intracellular calcium in hypertrophic smooth muscle from rat urinary bladder
    Anders Arner
    Department of Experimental Medical Science, Lund University, Lund, Sweden
    Scand J Urol Nephrol 41:270-7. 2007
    ..To explore whether infravesical outlet obstruction is associated with alterations in calcium activation of detrusor smooth muscle...
  40. ncbi Molecular modeling of the myosin-S1(A1) isoform
    Ewald M Aydt
    Revotar Biopharmaceuticals AG, Hennigsdorf, Germany
    J Struct Biol 159:158-63. 2007
    ..We show a rod-like 91 A structure being long enough to bridge the gap between the ELC core of myosin-S1 and the appropriate binding site of the ELC on the actin filament...
  41. ncbi Nitric oxide synthase upregulation and the predelivery blood pressure decrease in spontaneously hypertensive rats
    Heinrich Gompf
    HELIOS Klinikum-Berlin, , Medical Faculty of the Charit, Humboldt University of Berlin, Germany
    J Hypertens 20:255-61. 2002
    ..CONCLUSION : Increased NOS may explain the blood pressure decrease during late pregnancy in genetically hypertensive rats...
  42. ncbi Cardiac troponin I sense-antisense RNA duplexes in the myocardium
    Svenia Podlowski
    Medical Clinic I, Department of Cardiology, Humboldt-University (Charit, Ziegelstr. 5-9, D10117 Berlin, Germany
    J Cell Biochem 85:198-207. 2002
    ..Testing several oligonucleotides based on the natural antisense sequences, the optimal region for inhibition of translation was identified as being close to the translational start codon...