Markus R Meyer

Summary

Country: Germany

Publications

  1. doi request reprint Studies on the metabolism of the α-pyrrolidinophenone designer drug methylenedioxy-pyrovalerone (MDPV) in rat and human urine and human liver microsomes using GC-MS and LC-high-resolution MS and its detectability in urine by GC-MS
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    J Mass Spectrom 45:1426-42. 2010
  2. ncbi request reprint Toxicokinetics of novel psychoactive substances: characterization of N-acetyltransferase (NAT) isoenzymes involved in the phase II metabolism of 2C designer drugs
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany Electronic address
    Toxicol Lett 227:124-8. 2014
  3. ncbi request reprint Methylenedioxy designer drugs: mass spectrometric characterization of their glutathione conjugates by means of liquid chromatography-high-resolution mass spectrometry/mass spectrometry and studies on their glutathionyl transferase inhibition potency
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Saarland, Germany Electronic address
    Anal Chim Acta 822:37-50. 2014
  4. doi request reprint Dimethocaine, a synthetic cocaine analogue: studies on its in-vivo metabolism and its detectability in urine by means of a rat model and liquid chromatography-linear ion-trap (high-resolution) mass spectrometry
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg, Saar, Germany
    Anal Bioanal Chem 406:1845-54. 2014
  5. ncbi request reprint Dimethocaine, a synthetic cocaine derivative: studies on its in vitro metabolism catalyzed by P450s and NAT2
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Saar, Germany Electronic address
    Toxicol Lett 225:139-46. 2014
  6. doi request reprint A qualitative/quantitative approach for the detection of 37 tryptamine-derived designer drugs, 5 β-carbolines, ibogaine, and yohimbine in human urine and plasma using standard urine screening and multi-analyte approaches
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg, Saar, Germany
    Anal Bioanal Chem 406:225-37. 2014
  7. doi request reprint Ketamine-derived designer drug methoxetamine: metabolism including isoenzyme kinetics and toxicological detectability using GC-MS and LC-(HR-)MSn
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Anal Bioanal Chem 405:6307-21. 2013
  8. doi request reprint Studies on the metabolism and detectability of the designer drug β-naphyrone in rat urine using GC-MS and LC-HR-MS/MS
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical, Pharmacology and Toxicology, Saarland University, D 66421, Homburg, Saar, Germany
    Drug Test Anal 5:259-65. 2013
  9. doi request reprint Analytical toxicology of emerging drugs of abuse--an update
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Germany
    Ther Drug Monit 34:615-21. 2012
  10. doi request reprint Studies on the metabolism and detectability of the emerging drug of abuse diphenyl-2-pyrrolidinemethanol (D2PM) in rat urine using GC-MS and LC-HR-MS/MS
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology Toxicology, Saarland University, D 66421, Homburg Saar, Germany
    J Mass Spectrom 48:243-9. 2013

Collaborators

  • Hans H Maurer
  • Frank T Peters
  • Pierce Kavanagh
  • Frank Schuster
  • Peng Du
  • Simon D Brandt
  • Andrea E Schwaninger
  • Daniela Remane
  • Dirk K Wissenbach
  • Christoph Sauer
  • Jessica Welter
  • Golo M J Meyer
  • Armin A Weber
  • Josef Zapp
  • Marilyn A Huestis
  • Anika A Philipp
  • Andreas G Helfer
  • Julia Dinger
  • Robert S Goodwin
  • David A Gorelick
  • Erin A Kolbrich-Spargo
  • Allan J Barnes
  • Ralph Stuttmann
  • Julian A Michely
  • Deborah Montenarh
  • Carina S D Wink
  • Wolfgang Weinmann
  • Ehud Udi Wolf
  • Andreas H Ewald
  • Peter G M Zweipfenning
  • Siegfried W Zoerntlein
  • Peter Hilbert
  • Claudia Schafer
  • Giselher Fritschi
  • Claudia Haas

Detail Information

Publications57

  1. doi request reprint Studies on the metabolism of the α-pyrrolidinophenone designer drug methylenedioxy-pyrovalerone (MDPV) in rat and human urine and human liver microsomes using GC-MS and LC-high-resolution MS and its detectability in urine by GC-MS
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    J Mass Spectrom 45:1426-42. 2010
    ..Using recombinant human CYPs, CYP 2C19, CYP 2D6 and CYP 1A2 were found to catalyze this initial step. Finally, the STA allowed the detection of MDPV metabolites in the human urine samples...
  2. ncbi request reprint Toxicokinetics of novel psychoactive substances: characterization of N-acetyltransferase (NAT) isoenzymes involved in the phase II metabolism of 2C designer drugs
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany Electronic address
    Toxicol Lett 227:124-8. 2014
    ..In consequence, a slow acetylator phenotype or inhibition of NAT2 could lead to decreased N-acetylation and might lead to an increased risk of side effects caused by these novel psychoactive substances. ..
  3. ncbi request reprint Methylenedioxy designer drugs: mass spectrometric characterization of their glutathione conjugates by means of liquid chromatography-high-resolution mass spectrometry/mass spectrometry and studies on their glutathionyl transferase inhibition potency
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Saarland, Germany Electronic address
    Anal Chim Acta 822:37-50. 2014
    ..The DAA allowed the differentiation of the 2 and 5/6 isomers by confirmation of the postulated mass spectral fragments. Finally, the tested drugs and phase I metabolites showed no inhibition potency on GST activity. ..
  4. doi request reprint Dimethocaine, a synthetic cocaine analogue: studies on its in-vivo metabolism and its detectability in urine by means of a rat model and liquid chromatography-linear ion-trap (high-resolution) mass spectrometry
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg, Saar, Germany
    Anal Bioanal Chem 406:1845-54. 2014
    ..By use of GC-MS and LC-MS(n) standard urine-screening approaches (SUSAs), DMC and its metabolites could be detected in the urine samples...
  5. ncbi request reprint Dimethocaine, a synthetic cocaine derivative: studies on its in vitro metabolism catalyzed by P450s and NAT2
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Saar, Germany Electronic address
    Toxicol Lett 225:139-46. 2014
    ..However, a slow acetylation phenotype or inhibition of NAT2 could lead to decreased N-acetylation and hence leading to an increased risk of side effects caused by this arylamine...
  6. doi request reprint A qualitative/quantitative approach for the detection of 37 tryptamine-derived designer drugs, 5 β-carbolines, ibogaine, and yohimbine in human urine and plasma using standard urine screening and multi-analyte approaches
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg, Saar, Germany
    Anal Bioanal Chem 406:225-37. 2014
    ..A validated quantification in plasma according to international recommendation could be demonstrated for 33 out of 44 analytes. ..
  7. doi request reprint Ketamine-derived designer drug methoxetamine: metabolism including isoenzyme kinetics and toxicological detectability using GC-MS and LC-(HR-)MSn
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Anal Bioanal Chem 405:6307-21. 2013
    ..The enzyme kinetic studies showed that the initial metabolic step in humans, the N-deethylation, was catalyzed by CYP2B6 and CYP3A4. Both SUSAs using GC-MS or LC-MS(n) allowed monitoring an MXE intake in urine...
  8. doi request reprint Studies on the metabolism and detectability of the designer drug β-naphyrone in rat urine using GC-MS and LC-HR-MS/MS
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical, Pharmacology and Toxicology, Saarland University, D 66421, Homburg, Saar, Germany
    Drug Test Anal 5:259-65. 2013
    ..Assuming similar kinetics, an intake of naphyrone should be detectable in human urine mainly via its metabolites...
  9. doi request reprint Analytical toxicology of emerging drugs of abuse--an update
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Germany
    Ther Drug Monit 34:615-21. 2012
    ....
  10. doi request reprint Studies on the metabolism and detectability of the emerging drug of abuse diphenyl-2-pyrrolidinemethanol (D2PM) in rat urine using GC-MS and LC-HR-MS/MS
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology Toxicology, Saarland University, D 66421, Homburg Saar, Germany
    J Mass Spectrom 48:243-9. 2013
    ..Assuming similar kinetics, an intake of D2PM should be detectable in human urine mainly via its metabolites...
  11. doi request reprint Michaelis-Menten kinetic analysis of drugs of abuse to estimate their affinity to human P-glycoprotein
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Toxicol Lett 217:137-42. 2013
    ..As a consequence interactions with other drugs being P-gp substrates might be considered to be very likely and further studies should be encouraged...
  12. doi request reprint New cathinone-derived designer drugs 3-bromomethcathinone and 3-fluoromethcathinone: studies on their metabolism in rat urine and human liver microsomes using GC-MS and LC-high-resolution MS and their detectability in urine
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    J Mass Spectrom 47:253-62. 2012
    ..Following CYP enzyme kinetic studies, CYP2B6 was the most relevant enzyme for both the N-demethylation of 3-BMC and 3-FMC after in vitro-in vivo extrapolation...
  13. doi request reprint Current applications of high-resolution mass spectrometry in drug metabolism studies
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Anal Bioanal Chem 403:1221-31. 2012
    ..Finally, the advantages and limitations of these methods are discussed...
  14. pmc The breast feeding mother and xenon anaesthesia: four case reports. Breast feeding and xenon anaesthesia
    Ralph Stuttmann
    Department of Anaesthesiology Intensive and Emergency Medicine Pain Therapy, BG Kliniken Bergmannstrost, Merseburger Strasse 165, Halle Saale, 06112, Germany
    BMC Anesthesiol 10:1. 2010
    ..Four nursing mothers consented to anaesthesia for urgent surgery only on condition that their ability to breast feed would not be impaired...
  15. doi request reprint A validated GC-MS procedure for fast, simple, and cost-effective quantification of glycols and GHB in human plasma and their identification in urine and plasma developed for emergency toxicology
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421 Homburg Saar, Germany
    Anal Bioanal Chem 400:411-4. 2011
    ..Average analysis time from starting sample preparation until quantitative plasma results of approximately 35 min was achieved. This turnaround time is considered most appropriate for emergency cases...
  16. doi request reprint Development, validation, and application of a fast and simple GC-MS method for determination of some therapeutic drugs relevant in emergency toxicology
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Saarland, Homburg Saar, Germany
    Ther Drug Monit 33:649-53. 2011
    ..As they are no longer available, a fast, simple, and cost-effective quantitative gas chromatography-mass spectrometry (GC-MS) method was developed and fully validated for these drugs...
  17. doi request reprint Beta-keto amphetamines: studies on the metabolism of the designer drug mephedrone and toxicological detection of mephedrone, butylone, and methylone in urine using gas chromatography-mass spectrometry
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421 Homburg Saar, Germany
    Anal Bioanal Chem 397:1225-33. 2010
    ..Assuming similar kinetics in humans, the described STA procedure should be suitable for proof of an intake of the bk-designer drugs in human urine...
  18. doi request reprint Qualitative studies on the metabolism and the toxicological detection of the fentanyl-derived designer drugs 3-methylfentanyl and isofentanyl in rats using liquid chromatography-linear ion trap-mass spectrometry (LC-MS(n))
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421 Homburg, Saar, Germany
    Anal Bioanal Chem 402:1249-55. 2012
    ..However, in urine of rats after the administration of suspected recreational doses, the parent drugs could not be detected, but their common nor metabolite, which should therefore be the target for urine screening...
  19. doi request reprint The role of human hepatic cytochrome P450 isozymes in the metabolism of racemic 3,4-methylenedioxyethylamphetamine and its single enantiomers
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Drug Metab Dispos 37:1152-6. 2009
    ..In conclusion, different pharmacokinetic properties of MDEA enantiomers observed in vivo are therefore partially caused by P450-dependent enantioselective metabolism...
  20. doi request reprint Stereoselective differences in the cytochrome P450-dependent dealkylation and demethylenation of N-methyl-benzodioxolyl-butanamine (MBDB, Eden) enantiomers
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Biochem Pharmacol 77:1725-34. 2009
    ..In conclusion, the main enzyme responsible for MBDB metabolism after in vitro-in vivo correlation is CYP2D6, whereas CYP2C19 is the most enantioselective...
  21. doi request reprint New designer drug alpha-pyrrolidinovalerophenone (PVP): studies on its metabolism and toxicological detection in rat urine using gas chromatographic/mass spectrometric techniques
    Christoph Sauer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    J Mass Spectrom 44:952-64. 2009
    ..In addition, the involvement of nine different human cytochrome P450 (CYP) isoenzymes in the side chain hydroxylation of PVP was investigated and CYP 2B6, 2C19, 2D6, and 3A4 were found to catalyze this reaction...
  22. ncbi request reprint Identification of cytochrome P450 enzymes involved in the metabolism of the new designer drug 4'-methyl-alpha-pyrrolidinobutyrophenone
    Frank T Peters
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Building 46, D 66421 Homburg Saar, Germany
    Drug Metab Dispos 36:163-8. 2008
    ..From these data, it can be concluded that polymorphically expressed CYP2D6 is mainly responsible for MPBP hydroxylation...
  23. doi request reprint Investigations on the human hepatic cytochrome P450 isozymes involved in the metabolism of 3,4-methylenedioxy-amphetamine (MDA) and benzodioxolyl-butanamine (BDB) enantiomers
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Kirrberger Strasse 1, D 66421 Homburg Saar, Germany
    Toxicol Lett 190:54-60. 2009
    ..A preferred transformation of the S-enantiomer could be observed for the CYP2D6- and CYP3A4-catalyzed reactions...
  24. doi request reprint Investigations on the cytochrome P450 (CYP) isoenzymes involved in the metabolism of the designer drugs N-(1-phenyl cyclohexyl)-2-ethoxyethanamine and N-(1-phenylcyclohexyl)-2-methoxyethanamine
    Christoph Sauer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Kirrberger Strasse 1, D 66421 Homburg Saar, Germany
    Biochem Pharmacol 77:444-50. 2009
    ..At 1 microM PCMEA, CBP reduced metabolite formation in pooled HLM by 70% and at 10 microM PCMEA by 78%, respectively. In conclusion, the main metabolic step of both studied drugs was catalyzed by different CYPs...
  25. doi request reprint Identification of cytochrome P450 enzymes involved in the metabolism of the designer drugs N-(1-phenylcyclohexyl)-3-ethoxypropanamine and N-(1-phenylcyclohexyl)-3-methoxypropanamine
    Christoph Sauer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg, Saar, Germany
    Chem Res Toxicol 21:1949-55. 2008
    ..In conclusion, the main metabolic step was catalyzed by different P450s...
  26. doi request reprint Investigations on the stereoselectivity of the phase II metabolism of the 3,4-methylenedioxyethylamphetamine (MDEA) metabolites 3,4-dihydroxyethylamphetamine (DHEA) and 4-hydroxy-3-methoxyethylamphetamine (HMEA)
    Andrea E Schwaninger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Toxicol Lett 212:38-47. 2012
    ..In conclusion, the phase II metabolism might also contribute to the observed different pharmacokinetic properties of MDEA...
  27. doi request reprint Investigation on the enantioselectivity of the sulfation of the methylenedioxymethamphetamine metabolites 3,4-dihydroxymethamphetamine and 4-hydroxy-3-methoxymethamphetamine using the substrate-depletion approach
    Andrea E Schwaninger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Building 46, D 66421 Homburg Saar, Germany
    Drug Metab Dispos 39:1998-2002. 2011
    ..In conclusion, preferences for S-enantiomers were observed for DHMA sulfation, but not for HMMA sulfation...
  28. pmc Urinary excretion kinetics of 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) and its phase I and phase II metabolites in humans following controlled MDMA administration
    Andrea E Schwaninger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Clin Chem 57:1748-56. 2011
    ..Previous urinary excretion studies after controlled oral MDMA administration have been performed only after conjugate cleavage. Therefore, we investigated intact MDMA glucuronide and sulfate metabolite excretion...
  29. doi request reprint Studies on the in vivo contribution of human cytochrome P450s to the hepatic metabolism of glaucine, a new drug of abuse
    Golo M J Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Biochem Pharmacol 86:1497-506. 2013
    ..As glaucine was metabolized via three initial steps and different P450 isoforms were involved in the hepatic clearance of glaucine, a clinically relevant interaction with single inhibitors should not be expected. ..
  30. doi request reprint Enantioselectivity in the methylation of the catecholic phase I metabolites of methylenedioxy designer drugs and their capability to inhibit catechol-O-methyltransferase-catalyzed dopamine 3-methylation
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Chem Res Toxicol 22:1205-11. 2009
    ..Furthermore, the catecholic metabolites were identified to be uncompetitive inhibitors of the sCOMT localized in HLC...
  31. ncbi request reprint Biotechnological synthesis of drug metabolites using human cytochrome P450 2D6 heterologously expressed in fission yeast exemplified for the designer drug metabolite 4'-hydroxymethyl-alpha-pyrrolidinobutyrophenone
    Frank T Peters
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Biochem Pharmacol 74:511-20. 2007
    ..996). The rate of metabolite formation was slower in the earlier stages of incubation but then increased. For HO-MPBP, it became constant in the time interval of 2.5-34 h (R(2)>998)...
  32. ncbi request reprint The role of human UDP-glucuronyltransferases on the formation of the methylenedioxymethamphetamine (ecstasy) phase II metabolites R- and S-3-methoxymethamphetamine 4-O-glucuronides
    Andrea E Schwaninger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Drug Metab Dispos 37:2212-20. 2009
    ..In conclusion, the O-glucuronidation of HMMA in vivo should not be expected to be enantioselective, and the different pharmacokinetic properties may not be caused directly by glucuronidation...
  33. doi request reprint The role of human hepatic cytochrome P450 isozymes in the metabolism of racemic 3,4-methylenedioxy-methamphetamine and its enantiomers
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Building 46, D 66421 Homburg Saar, Germany
    Drug Metab Dispos 36:2345-54. 2008
    ..None of the other isozymes showed major preferences for certain enantiomers. In conclusion, therefore, the different pharmacokinetic properties of the MDMA enantiomers may be caused by enantioselective metabolism by CYP2C19 and CYP2D6...
  34. doi request reprint Drugs of abuse screening in urine as part of a metabolite-based LC-MSn screening concept
    Dirk K Wissenbach
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Anal Bioanal Chem 400:3481-9. 2011
    ..The presented LC-MS(n) method complements the well-established gas chromatography-mass spectroscopy procedure in the authors' laboratory...
  35. doi request reprint Sulfation of the 3,4-methylenedioxymethamphetamine (MDMA) metabolites 3,4-dihydroxymethamphetamine (DHMA) and 4-hydroxy-3-methoxymethamphetamine (HMMA) and their capability to inhibit human sulfotransferases
    Andrea E Schwaninger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Kirrberger Strasse 1, D 66421 Homburg Saar, Germany
    Toxicol Lett 202:120-8. 2011
    ..In conclusion, the presented data indicated that sulfation of HMMA should be the major conjugation reaction observed in humans. Furthermore, both, DHMA and HMMA, were identified as inhibitors of dopamine sulfation...
  36. doi request reprint Towards a universal LC-MS screening procedure - can an LIT LC-MS(n) screening approach and reference library be used on a quadrupole-LIT hybrid instrument?
    Dirk K Wissenbach
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421, Homburg, Saar, Germany
    J Mass Spectrom 47:66-71. 2012
    ..These should be overcome by further optimizations regarding DDA settings for better sensitivity and further library modifications to reduce spectra mismatches...
  37. doi request reprint Automated mass spectral deconvolution and identification system for GC-MS screening for drugs, poisons, and metabolites in urine
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Clin Chem 56:575-84. 2010
    ....
  38. doi request reprint Fast and simple procedure for liquid-liquid extraction of 136 analytes from different drug classes for development of a liquid chromatographic-tandem mass spectrometric quantification method in human blood plasma
    Daniela Remane
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421 Homburg Saar, Germany
    Anal Bioanal Chem 397:2303-14. 2010
    ..Matrix effects with more than 25% were observed for 18 analytes. The results were acceptable for 119 analytes at high concentrations...
  39. ncbi request reprint Studies on the metabolism and toxicological detection of the new designer drug 4'-methyl-alpha-pyrrolidinobutyrophenone (MPBP) in rat urine using gas chromatography-mass spectrometry
    Frank T Peters
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Saarland, D 66421 Homburg Saar, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 824:81-91. 2005
    ..Assuming similar metabolism and dosages in humans, an intake of MPBP should be detectable via its metabolites in urine...
  40. pmc Development and validation of LC-HRMS and GC-NICI-MS methods for stereoselective determination of MDMA and its phase I and II metabolites in human urine
    Andrea E Schwaninger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    J Mass Spectrom 46:603-14. 2011
    ....
  41. ncbi request reprint Metabolism of designer drugs of abuse: an updated review
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland, University, Homburg Saar, Germany
    Curr Drug Metab 11:468-82. 2010
    ..Papers have been considered and reviewed on the identification of in vivo or in vitro human or animal metabolites and the cytochrome P450 or monoamineoxidase isoenzyme-dependent metabolism...
  42. doi request reprint Systematic investigation of ion suppression and enhancement effects of fourteen stable-isotope-labeled internal standards by their native analogues using atmospheric-pressure chemical ionization and electrospray ionization and the relevance for multi-anal
    Daniela Remane
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Rapid Commun Mass Spectrom 24:859-67. 2010
    ..In conclusion, only SIL-ISs should be selected for which no suppression and enhancement effects can be observed. If not enough ISs are free of ionization interferences, a different ionization technique should be considered...
  43. doi request reprint Development of the first metabolite-based LC-MS(n) urine drug screening procedure-exemplified for antidepressants
    Dirk K Wissenbach
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg, Saar, Germany
    Anal Bioanal Chem 400:79-88. 2011
    ..Furthermore, the detection of metabolites confirms the body passage. The presented LC-MS(n) method complements established GC-MS or LC-MS procedures in the authors' lab...
  44. doi request reprint Ion suppression and enhancement effects of co-eluting analytes in multi-analyte approaches: systematic investigation using ultra-high-performance liquid chromatography/mass spectrometry with atmospheric-pressure chemical ionization or electrospray ionizat
    Daniela Remane
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Rapid Commun Mass Spectrom 24:3103-8. 2010
    ..In conclusion, ion suppression and enhancement tests are essential during method development and validation in LC/MS/MS multi-analyte procedures, with special regards to co-eluting analytes...
  45. doi request reprint Absorption, distribution, metabolism and excretion pharmacogenomics of drugs of abuse
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg Saar, Germany
    Pharmacogenomics 12:215-33. 2011
    ....
  46. doi request reprint Full validation and application of an ultra high performance liquid chromatographic-tandem mass spectrometric procedure for target screening and quantification of 34 antidepressants in human blood plasma as part of a comprehensive multi-analyte approach
    Daniela Remane
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg Saar, Germany
    Anal Bioanal Chem 400:2093-107. 2011
    ..Furthermore, it could be shown that time- and cost-saving one-point calibration was applicable for 21 drugs for daily routine and especially in emergency cases...
  47. pmc Stereoselective urinary MDMA (ecstasy) and metabolites excretion kinetics following controlled MDMA administration to humans
    Andrea E Schwaninger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Saar, Germany
    Biochem Pharmacol 83:131-8. 2012
    ..R/S ratios could help to roughly estimate time of MDMA ingestion and therefore, improve interpretation of MDMA and metabolite urinary concentrations in clinical and forensic toxicology...
  48. ncbi request reprint Gas chromatography-mass spectrometry detection of a norfluoxetine artifact in hydrolyzed urine samples may falsely indicate tranylcypromine ingestion
    Andrea E Schwaninger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Building 46, D 66421 Homburg Saar, Germany
    J Anal Toxicol 34:45-8. 2010
    ..Although this spectrum shows nearly the same fragmentation patterns as that of acetylated tranylcypromine, both compounds could finally be differentiated by their retention indices and by using the positive-ion chemical ionization mode...
  49. doi request reprint Studies on the metabolism and the detectability of 4-methyl-amphetamine and its isomers 2-methyl-amphetamine and 3-methyl-amphetamine in rat urine using GC-MS and LC-(high-resolution)-MSn
    Jessica Welter
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421, Homburg Saar, Germany
    Anal Bioanal Chem 406:1957-74. 2014
    ..Differentiation of the isomers to confirm the intake of a specific isomer was possible with an additional workup in rat urine...
  50. doi request reprint 2-methiopropamine, a thiophene analogue of methamphetamine: studies on its metabolism and detectability in the rat and human using GC-MS and LC-(HR)-MS techniques
    Jessica Welter
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg Saar, Germany
    Anal Bioanal Chem 405:3125-35. 2013
    ..Ingestion of 2-MPA could also be detected by both protocols in an authentic human urine sample...
  51. doi request reprint Studies on the metabolism and toxicological detection of glaucine, an isoquinoline alkaloid from Glaucium flavum (Papaveraceae), in rat urine using GC-MS, LC-MS(n) and LC-high-resolution MS(n)
    Golo M J Meyer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421, Homburg, Saar, Germany
    J Mass Spectrom 48:24-41. 2013
    ..Both allowed confirming an intake of glaucine in rat urine after a dose of 2 mg/kg body mass corresponding to a common abuser's dose...
  52. doi request reprint Current status of hyphenated mass spectrometry in studies of the metabolism of drugs of abuse, including doping agents
    Markus R Meyer
    Department of Experimental and Clinical Toxicology, Saarland University, Homburg Saar, Germany
    Anal Bioanal Chem 402:195-208. 2012
    ..In conclusion, the reviewed papers showed that both GC-MS and LC-MS still have important roles to play in research into the metabolism of drugs of abuse, including doping agents...
  53. doi request reprint Monitoring of kratom or Krypton intake in urine using GC-MS in clinical and forensic toxicology
    Anika A Philipp
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421 Homburg, Saar, Germany
    Anal Bioanal Chem 400:127-35. 2011
    ..As the metabolism in humans was similar, this procedure should be suitable to prove an intake of kratom or Krypton...
  54. ncbi request reprint Ultra high performance liquid chromatographic-tandem mass spectrometric multi-analyte procedure for target screening and quantification in human blood plasma: validation and application for 31 neuroleptics, 28 benzodiazepines, and Z-drugs
    Daniela Remane
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Saar, Germany
    Anal Bioanal Chem 401:1341-52. 2011
    ..Cost- and time-saving one-point calibration was applicable only for half of the analytes. The applicability was successfully shown for most of the drugs by analyzing authentic plasma samples and external quality control samples...
  55. ncbi request reprint Development and validation of a liquid-chromatography high-resolution tandem mass spectrometry approach for quantification of nine cytochrome P450 (CYP) model substrate metabolites in an in vitro CYP inhibition cocktail
    Julia Dinger
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg, Saar, Germany
    Anal Bioanal Chem 406:4453-64. 2014
    ..In conclusion, the presented assay was suitable for the quantification of the model substrate metabolites and could be used for the development of a CYP inhibition assay for testing most CYPs and a wide range of drugs of abuse. ..
  56. doi request reprint Application of a UHPLC MS/MS-based multianalyte approach for screening and validated quantification of drugs in human blood plasma often requested in the context of brain death diagnosis
    Daniela Remane
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Saarland, Homburg, Saarland, Germany
    Ther Drug Monit 36:257-60. 2014
    ....
  57. doi request reprint Direct analysis of the mushroom poisons α- and β-amanitin in human urine using a novel on-line turbulent flow chromatography mode coupled to liquid chromatography-high resolution-mass spectrometry/mass spectrometry
    Andreas G Helfer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D 66421 Homburg, Saar, Germany
    J Chromatogr A 1325:92-8. 2014
    ....