Eric Metzen

Summary

Country: Germany

Publications

  1. pmc Nitric oxide impairs normoxic degradation of HIF-1alpha by inhibition of prolyl hydroxylases
    Eric Metzen
    Institute of Physiology, University of Luebeck, Germany
    Mol Biol Cell 14:3470-81. 2003
  2. ncbi Inhibition of mitochondrial respiration elevates oxygen concentration but leaves regulation of hypoxia-inducible factor (HIF) intact
    Kathrin Doege
    Institute of Physiology, University of Lubeck, Lubeck, Germany
    Blood 106:2311-7. 2005
  3. ncbi Intracellular localisation of human HIF-1 alpha hydroxylases: implications for oxygen sensing
    Eric Metzen
    Institute of Physiology, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    J Cell Sci 116:1319-26. 2003
  4. ncbi HIF hydroxylation and cellular oxygen sensing
    Eric Metzen
    Institute of Physiology, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    Biol Chem 385:223-30. 2004
  5. pmc Regulation of the prolyl hydroxylase domain protein 2 (phd2/egln-1) gene: identification of a functional hypoxia-responsive element
    Eric Metzen
    Institute of Physiology, University of Luebeck, Ratzeburger Allee 160, D23538 Luebeck, Germany
    Biochem J 387:711-7. 2005
  6. pmc Hypoxia-inducible factor-1 (HIF-1) promotes its degradation by induction of HIF-alpha-prolyl-4-hydroxylases
    Jan H Marxsen
    Department of Internal Medicine I, University of Luebeck, Ratzeburger Allee 160, D 23538 Luebeck, Germany
    Biochem J 381:761-7. 2004
  7. pmc The function of hypoxia-inducible factor (HIF) is independent of the endoplasmic reticulum protein OS-9
    Ulf Brockmeier
    Institute of Physiology, University of Duisburg Essen, Essen, Germany
    PLoS ONE 6:e19151. 2011
  8. ncbi Review: hypoxia-inducible factor-1 (HIF-1): a novel transcription factor in immune reactions
    Thomas Hellwig-Burgel
    Institute of Physiology, University of Luebeck, D 23538 Luebeck, Germany
    J Interferon Cytokine Res 25:297-310. 2005
  9. doi Nuclear translocation of hypoxia-inducible factors (HIFs): involvement of the classical importin alpha/beta pathway
    Reinhard Depping
    Department of Physiology, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    Biochim Biophys Acta 1783:394-404. 2008
  10. doi Stabilisation and knockdown of HIF--two distinct ways comparably important in radiotherapy
    Mareike Ströfer
    Department of Physiology, Center for Structural and Cell Biology in Medicine, University of Lubeck, Lubeck, Germany
    Cell Physiol Biochem 28:805-12. 2011

Collaborators

Detail Information

Publications18

  1. pmc Nitric oxide impairs normoxic degradation of HIF-1alpha by inhibition of prolyl hydroxylases
    Eric Metzen
    Institute of Physiology, University of Luebeck, Germany
    Mol Biol Cell 14:3470-81. 2003
    ..We conclude that GSNO-attenuated prolyl hydroxylase activity accounts for HIF-1alpha accumulation under conditions of NO formation during normoxia and that PHD activity is subject to regulation by NO...
  2. ncbi Inhibition of mitochondrial respiration elevates oxygen concentration but leaves regulation of hypoxia-inducible factor (HIF) intact
    Kathrin Doege
    Institute of Physiology, University of Lubeck, Lubeck, Germany
    Blood 106:2311-7. 2005
    ..Our data demonstrate that reduction of oxygen consumption reduces the O2 gradient in conventional cell cultures, causing elevation of the cellular O2 concentration, which leads to degradation of HIF-alpha...
  3. ncbi Intracellular localisation of human HIF-1 alpha hydroxylases: implications for oxygen sensing
    Eric Metzen
    Institute of Physiology, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    J Cell Sci 116:1319-26. 2003
    ..Endogenous PHD2mRNA and PHD3mRNA were hypoxia-inducible, whereas expression of PHD1mRNA and FIH-1mRNA was oxygen independent. We propose that PHDs and FIH-1 form an oxygen sensor cascade of distinct subcellular localisation...
  4. ncbi HIF hydroxylation and cellular oxygen sensing
    Eric Metzen
    Institute of Physiology, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    Biol Chem 385:223-30. 2004
    ..Regulation of the amount of hydroxylase protein, and the supply of other co-substrates and co-factors, particularly the cellular availability of iron, also contribute to tuning the physiological response to hypoxia...
  5. pmc Regulation of the prolyl hydroxylase domain protein 2 (phd2/egln-1) gene: identification of a functional hypoxia-responsive element
    Eric Metzen
    Institute of Physiology, University of Luebeck, Ratzeburger Allee 160, D23538 Luebeck, Germany
    Biochem J 387:711-7. 2005
    ..We conclude that the phd2 gene is transcribed exclusively from the downstream promoter that contains a functional hypoxia-responsive, cis-regulatory element. Our results establish that PHD2 is a direct HIF target gene...
  6. pmc Hypoxia-inducible factor-1 (HIF-1) promotes its degradation by induction of HIF-alpha-prolyl-4-hydroxylases
    Jan H Marxsen
    Department of Internal Medicine I, University of Luebeck, Ratzeburger Allee 160, D 23538 Luebeck, Germany
    Biochem J 381:761-7. 2004
    ..We propose a direct, negative regulatory mechanism, which limits accumulation of HIF-1alpha in hypoxia and leads to accelerated degradation on reoxygenation after long-term hypoxia...
  7. pmc The function of hypoxia-inducible factor (HIF) is independent of the endoplasmic reticulum protein OS-9
    Ulf Brockmeier
    Institute of Physiology, University of Duisburg Essen, Essen, Germany
    PLoS ONE 6:e19151. 2011
    ..We conclude that OS-9 plays no direct functional role in HIF degradation since physical interaction of OS-9 with oxygen sensing HIF prolyl hydroxylases cannot occur in vivo due to their different subcellular localization...
  8. ncbi Review: hypoxia-inducible factor-1 (HIF-1): a novel transcription factor in immune reactions
    Thomas Hellwig-Burgel
    Institute of Physiology, University of Luebeck, D 23538 Luebeck, Germany
    J Interferon Cytokine Res 25:297-310. 2005
    ..The pharmacologic activation of the HIF-1 complex can be desirable in ischemic and inflammatory disorders. In contrast, HIF-1 blockade may be beneficial to prevent tumor angiogenesis and tumor growth...
  9. doi Nuclear translocation of hypoxia-inducible factors (HIFs): involvement of the classical importin alpha/beta pathway
    Reinhard Depping
    Department of Physiology, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    Biochim Biophys Acta 1783:394-404. 2008
    ..In contrast, the supposed N-terminal NLS is not effective. Our findings provide new insight into the mechanism of the regulation of nuclear transport of hypoxia-inducible factors...
  10. doi Stabilisation and knockdown of HIF--two distinct ways comparably important in radiotherapy
    Mareike Ströfer
    Department of Physiology, Center for Structural and Cell Biology in Medicine, University of Lubeck, Lubeck, Germany
    Cell Physiol Biochem 28:805-12. 2011
    ..The aim of the study was to examine cell type-specific effects of HIF-1α and -2α knockdown or oxygen-independent HIF-stabilisation on radiosensitivity...
  11. ncbi Lack of functional erythropoietin receptors of cancer cell lines
    Magdalena Laugsch
    Institute of Physiology, University of Luebeck, D 23538 Luebeck, Germany
    Int J Cancer 122:1005-11. 2008
    ..Apart from UT7/Epo, none of the tumor cell lines responded to Epo treatment with phosphorylation of signaling molecules or with cell proliferation...
  12. pmc Enzyme substrate recognition in oxygen sensing: how the HIF trap snaps
    Eric Metzen
    Institute of Physiology, University of Lubeck, Ratzeburger Allee 160, D23538 Lübeck, Federal Republic of Germany
    Biochem J 408:e5-6. 2007
    ..This elegant study improves our understanding of the interaction of the oxygen-sensing PHDs/EGLNs with their substrates, which include, but are not limited to, the HIFalpha proteins...
  13. doi Factor inhibiting HIF-1 (FIH-1) modulates protein interactions of apoptosis-stimulating p53 binding protein 2 (ASPP2)
    Kirsten Janke
    Institute of Physiology, Faculty of Medicine, University Duisburg Essen, 45147 Essen, Germany
    J Cell Sci 126:2629-40. 2013
    ..The large cellular pool of ARD-containing proteins suggests that FIH-1 can affect a broad range of cellular functions and signalling pathways under certain conditions, for example, in response to severe hypoxia. ..
  14. doi New insight into the SDF-1/CXCR4 axis in a breast carcinoma model: hypoxia-induced endothelial SDF-1 and tumor cell CXCR4 are required for tumor cell intravasation
    Fengyan Jin
    The First Hospital of Jilin University, Changchun, China
    Mol Cancer Res 10:1021-31. 2012
    ..Because both proteins were readily identifiable in a significant fraction of human breast cancer samples by immunohistochemistry, CXCR4 may constitute a molecular target for therapy when both, SDF-1, and CXCR4 are expressed...
  15. pmc Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation
    Krista Rantanen
    Turku Centre for Biotechnology, Turku University and Abo Akademi University, FIN 29521 Turku, Finland
    Mol Biol Cell 19:2231-40. 2008
    ..The data demonstrates the cellular oxygen sensor PHD3 as a regulator of protein aggregation in response to varying oxygen availability...
  16. ncbi Nitric oxide reverses desferrioxamine- and hypoxia-evoked HIF-1alpha accumulation--implications for prolyl hydroxylase activity and iron
    Melvin Callapina
    Faculty of Medicine, Institute of Biochemistry I, Johann Wolfgang Goethe University, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    Exp Cell Res 306:274-84. 2005
    ..Variables that allow efficient PHD activation such as oxygen availability, iron content, or cofactor accessibility at that end allow NO to modulate HIF-1alpha accumulation...
  17. ncbi Chelation of cellular calcium modulates hypoxia-inducible gene expression through activation of hypoxia-inducible factor-1alpha
    Utta Berchner-Pfannschmidt
    Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
    J Biol Chem 279:44976-86. 2004
    ..Instead, effective chelation of cellular calcium caused the accumulation of HIF-1alpha protein through inhibition of HIF-prolyl hydroxylases and activated HIF-1-dependent gene expression under normoxic conditions...
  18. ncbi Optical analysis of the HIF-1 complex in living cells by FRET and FRAP
    Christoph Wotzlaw
    Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
    FASEB J 21:700-7. 2007
    ..2 nm, but even the N and C terminus of the HIF-1 complex were not further apart than 7.4 nm. Our data suggest a more compact 3-dimensional organization of the HIF complex than described so far by 2-dimensional models...