Thomas C Mettenleiter

Summary

Country: Germany

Publications

  1. ncbi request reprint Five unique open reading frames of infectious laryngotracheitis virus are expressed during infection but are dispensable for virus replication in cell culture
    Jutta Veits
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17493 Greifswald Insel Riems, Germany
    J Gen Virol 84:1415-25. 2003
  2. ncbi request reprint Intriguing interplay between viral proteins during herpesvirus assembly or: the herpesvirus assembly puzzle
    Thomas C Mettenleiter
    Institute of Molecular Biology, Friedrich Loeffler Institut, Insel Riems, Germany
    Vet Microbiol 113:163-9. 2006
  3. doi request reprint The way out: what we know and do not know about herpesvirus nuclear egress
    Thomas C Mettenleiter
    Institute of Molecular Biology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    Cell Microbiol 15:170-8. 2013
  4. pmc Correlative VIS-fluorescence and soft X-ray cryo-microscopy/tomography of adherent cells
    Christoph Hagen
    Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK
    J Struct Biol 177:193-201. 2012
  5. pmc Effects of polymorphisms in ovine and caprine prion protein alleles on cell-free conversion
    Martin Eiden
    Institute for Novel and Emerging Infectious Diseases at the Friedrich Loeffler Institut, Federal Research Institute for Animal Health, D 17493 Greifswald Insel Riems, Germany
    Vet Res 42:30. 2011
  6. pmc Chemosensory properties of murine nasal and cutaneous trigeminal neurons identified by viral tracing
    Nils Damann
    Lehrstuhl fur Zellphysiologie, Ruhr Universitat, Bochum, Germany
    BMC Neurosci 7:46. 2006
  7. ncbi request reprint Herpesvirus assembly: a tale of two membranes
    Thomas C Mettenleiter
    Friedrich Loeffler Institut, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    Curr Opin Microbiol 9:423-9. 2006
  8. doi request reprint Herpesvirus assembly: an update
    Thomas C Mettenleiter
    Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    Virus Res 143:222-34. 2009
  9. pmc Relevance of the interaction between alphaherpesvirus UL3.5 and UL48 proteins for virion maturation and neuroinvasion
    Walter Fuchs
    Friedrich Loeffler Institut, Institute of Molecular Biology, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 81:9307-18. 2007
  10. pmc Intracellular localization of the pseudorabies virus large tegument protein pUL36
    Britta S Möhl
    Institutes of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    J Virol 83:9641-51. 2009

Detail Information

Publications109 found, 100 shown here

  1. ncbi request reprint Five unique open reading frames of infectious laryngotracheitis virus are expressed during infection but are dispensable for virus replication in cell culture
    Jutta Veits
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17493 Greifswald Insel Riems, Germany
    J Gen Virol 84:1415-25. 2003
    ..These findings indicate that the ILTV-specific ORF A to ORF E genes might be important for virus replication in the natural host organism...
  2. ncbi request reprint Intriguing interplay between viral proteins during herpesvirus assembly or: the herpesvirus assembly puzzle
    Thomas C Mettenleiter
    Institute of Molecular Biology, Friedrich Loeffler Institut, Insel Riems, Germany
    Vet Microbiol 113:163-9. 2006
    ..This review summarizes recent advances in our understanding of the complex protein-protein interactions involved in herpesvirus assembly and egress...
  3. doi request reprint The way out: what we know and do not know about herpesvirus nuclear egress
    Thomas C Mettenleiter
    Institute of Molecular Biology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    Cell Microbiol 15:170-8. 2013
    ..Thus, herpesviruses appear to subvert a hitherto cryptic cellular pathway for translocation of capsids from the nucleus to the cytosol...
  4. pmc Correlative VIS-fluorescence and soft X-ray cryo-microscopy/tomography of adherent cells
    Christoph Hagen
    Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK
    J Struct Biol 177:193-201. 2012
    ..Taken together, our results demonstrate new possibilities to study the role of specific proteins in substructures of adherent cells, especially of the nucleus in toto, accessible to electron microscopy in thinned samples only...
  5. pmc Effects of polymorphisms in ovine and caprine prion protein alleles on cell-free conversion
    Martin Eiden
    Institute for Novel and Emerging Infectious Diseases at the Friedrich Loeffler Institut, Federal Research Institute for Animal Health, D 17493 Greifswald Insel Riems, Germany
    Vet Res 42:30. 2011
    ..Moreover, we could demonstrate a dominant-negative inhibition of prion polymorphisms associated with high susceptibility by alleles linked to low susceptibility in vitro...
  6. pmc Chemosensory properties of murine nasal and cutaneous trigeminal neurons identified by viral tracing
    Nils Damann
    Lehrstuhl fur Zellphysiologie, Ruhr Universitat, Bochum, Germany
    BMC Neurosci 7:46. 2006
    ..Thus, functional properties of primary trigeminal afferents depending on the area of innervation remain largely unclear...
  7. ncbi request reprint Herpesvirus assembly: a tale of two membranes
    Thomas C Mettenleiter
    Friedrich Loeffler Institut, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    Curr Opin Microbiol 9:423-9. 2006
    ..Thus, herpesvirus morphogenesis proceeds in two different cellular compartments, involving different viral and cellular proteins...
  8. doi request reprint Herpesvirus assembly: an update
    Thomas C Mettenleiter
    Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    Virus Res 143:222-34. 2009
    ..C., 2004. Budding events in herpesvirus morphogenesis. Virus Res. 106, 167-180]...
  9. pmc Relevance of the interaction between alphaherpesvirus UL3.5 and UL48 proteins for virion maturation and neuroinvasion
    Walter Fuchs
    Friedrich Loeffler Institut, Institute of Molecular Biology, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 81:9307-18. 2007
    ..5 homologs, yield conclusive evidence for the incorporation of a proteolytically processed pUL3.5 into PrV virions, and demonstrate the importance of both proteins for neuroinvasion and neurovirulence of PrV...
  10. pmc Intracellular localization of the pseudorabies virus large tegument protein pUL36
    Britta S Möhl
    Institutes of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    J Virol 83:9641-51. 2009
    ....
  11. ncbi request reprint The UL47 gene of avian infectious laryngotracheitis virus is not essential for in vitro replication but is relevant for virulence in chickens
    Dorothee Helferich
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    J Gen Virol 88:732-42. 2007
    ..As all immunized animals were protected against subsequent challenge infection with virulent ILTV, the UL47 deletion mutant might be suitable as a live-virus vaccine...
  12. doi request reprint Identification of envelope protein pORF81 of koi herpesvirus
    Daniela Rosenkranz
    Institute of Molecular Biology, Federal Research Institute for Animal Health, 17493 Greifswald Insel Riems, Germany
    J Gen Virol 89:896-900. 2008
    ..The antiserum was also suitable for the detection of pORF81 in sections of gills, kidneys, hepatopancreas and skin of KHV-infected carp by immunohistochemistry...
  13. pmc Characterization of pseudorabies virus (PrV) cleavage-encapsidation proteins and functional complementation of PrV pUL32 by the homologous protein of herpes simplex virus type 1
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 83:3930-43. 2009
    ..However, trans-complementation of UL32-negative HSV-1 by the PrV protein was not observed...
  14. pmc Random transposon-mediated mutagenesis of the essential large tegument protein pUL36 of pseudorabies virus
    Britta S Möhl
    Institutes of Molecular, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    J Virol 84:8153-62. 2010
    ..In summary, these studies extend and refine previous analyses of PrV pUL36 and demonstrate the different sensitivities of different regions of the protein to functional loss by insertion...
  15. doi request reprint Phenotypic similarities and differences between UL37-deleted pseudorabies virus and herpes simplex virus type 1
    Tobias Leege
    Institute of Molecular Biology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Gen Virol 90:1560-8. 2009
    ..Trans-complementation of respective deletion mutants with the heterologous pUL37 did not ensue. These data demonstrate that the conserved pUL37 in HSV-1 and PrV have similar but distinct functions...
  16. pmc Functional analysis of the pseudorabies virus UL51 protein
    Barbara G Klupp
    Institute of Molecular Biology, Friedrich Loeffler Institut, Boddenblick 5a, Greifswald Insel Riems D 17493, Germany
    J Virol 79:3831-40. 2005
    ..In conclusion, both conserved tegument proteins, either singly or in combination, are involved in virion morphogenesis in the cytoplasm but are not essential for viral replication in vitro and in vivo...
  17. ncbi request reprint Identification of transcripts and protein products of the UL31, UL37, UL46, UL47, UL48, UL49 and US4 gene homologues of avian infectious laryngotracheitis virus
    Dorothee Helferich
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    J Gen Virol 88:719-31. 2007
    ..Taken together, these results indicate that the functions of all of the investigated ILTV proteins are related to those of their homologues in other alphaherpesviruses...
  18. ncbi request reprint The UL4 gene of pseudorabies virus encodes a minor infected-cell protein that is dispensable for virus replication
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    J Gen Virol 87:2517-25. 2006
    ..Thus, UL4 encodes a non-structural protein of PrV that enhances virion formation but is not essential for PrV replication in vitro or in vivo...
  19. doi request reprint Analysis of pseudorabies and herpes simplex virus recombinants simultaneously lacking the pUL17 and pUL25 components of the C-capsid specific component
    Jana Kuhn
    Institute of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    Virus Res 153:20-8. 2010
    ..Thus, the protein complexes of PrV and HSV-1 either possess distinct functions, or require interactions with other viral proteins which are impaired in a heterologous context...
  20. pmc Highly pathogenic H5N1 influenza viruses carry virulence determinants beyond the polybasic hemagglutinin cleavage site
    Jessica Bogs
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    PLoS ONE 5:e11826. 2010
    ..Moreover, besides the polybasic cleavage site, the additional virulence determinants of H5N1 HPAIV are located within the HA itself and in other viral proteins...
  21. pmc Mutagenesis of the active-site cysteine in the ubiquitin-specific protease contained in large tegument protein pUL36 of pseudorabies virus impairs viral replication in vitro and neuroinvasion in vivo
    Sindy Böttcher
    Friedrich Loeffler Institut, Institute of Molecular Biology, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 82:6009-16. 2008
    ..Thus, the DUB activity is important for PrV replication in vitro and for neuroinvasion in mice...
  22. pmc Translocation of incoming pseudorabies virus capsids to the cell nucleus is delayed in the absence of tegument protein pUL37
    Mirjam Krautwald
    Institute of Molecular Biology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 83:3389-96. 2009
    ..In contrast, phenotypically complemented pUL37-containing virions of PrV-DeltaUL37/UL35GFP exhibited wild type-like entry kinetics. Thus, the presence of pUL37 is required for rapid nuclear translocation of incoming nucleocapsids...
  23. pmc Newcastle disease virus expressing H5 hemagglutinin gene protects chickens against Newcastle disease and avian influenza
    Jutta Veits
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Boddenblick 5a, D 17493 Greifswald Insel Riems, Germany
    Proc Natl Acad Sci U S A 103:8197-202. 2006
    ..Therefore, recombinant NDVH5m is suitable as a bivalent vaccine against NDV and AIV and may be used as marker vaccine for the control of avian influenza...
  24. doi request reprint Protection of cats against lethal influenza H5N1 challenge infection
    Thomas W Vahlenkamp
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, 17493 Greifswald Insel Riems, Germany
    J Gen Virol 89:968-74. 2008
    ....
  25. pmc Nuclear envelope breakdown can substitute for primary envelopment-mediated nuclear egress of herpesviruses
    Barbara G Klupp
    Friedrich Loeffler Institut, Institute of Molecular Biology, Greifswald Insel Riems, Germany
    J Virol 85:8285-92. 2011
    ..However, nuclear egress exhibits a strong bias for nucleocapsids, thereby also functioning as a quality control checkpoint which is abolished by herpesvirus-induced nuclear envelope breakdown...
  26. ncbi request reprint Vaccination with Newcastle disease virus vectored vaccine protects chickens against highly pathogenic H7 avian influenza virus
    Diana Schröer
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Südufer 10, D 17493 Greifswald Insel Riems, Germany
    Avian Dis 53:190-7. 2009
    ..Furthermore, the immunized birds developed antibodies against the AIV nucleoprotein after challenge infection. Thus, NDVH7m could be used as a marker vaccine against subtype H7 avian influenza...
  27. pmc Identification of functional domains within the essential large tegument protein pUL36 of pseudorabies virus
    Sindy Böttcher
    Institute of Molecular Biology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 81:13403-11. 2007
    ..Thus, we identify several domains within the PrV UL36 protein, which, though not essential, are nevertheless important for virus replication...
  28. pmc Effects of simultaneous deletion of pUL11 and glycoprotein M on virion maturation of herpes simplex virus type 1
    Tobias Leege
    Institutes of Molecular Biology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    J Virol 83:896-907. 2009
    ..The defects of HSV-1DeltaUL11 and HSV-1DeltaUL11/gM could be partially corrected in trans by pUL11 of PrV. Thus, our data indicate that PrV and HSV-1 pUL11 and gM exhibit similar functions in cytoplasmic steps of virion assembly...
  29. pmc Reversion of PB2-627E to -627K during replication of an H5N1 Clade 2.2 virus in mammalian hosts depends on the origin of the nucleoprotein
    Jessica Bogs
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 85:10691-8. 2011
    ..However, the prompt reversion to PB2-627K in MDCK cells and mice suggests that the clade 2.2 H5N1 HPAIV may have had a history of intermediate mammalian hosts...
  30. pmc Ultrastructural analysis of virion formation and anterograde intraaxonal transport of the alphaherpesvirus pseudorabies virus in primary neurons
    Christina Maresch
    Institutes of Molecular Biology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 84:5528-39. 2010
    ..Our data thus support intraaxonal anterograde transport of enveloped PrV virions within vesicles following the "married model."..
  31. doi request reprint Protection of chickens against H5N1 highly pathogenic avian influenza virus infection by live vaccination with infectious laryngotracheitis virus recombinants expressing H5 hemagglutinin and N1 neuraminidase
    Sophia P Pavlova
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    Vaccine 27:773-85. 2009
    ..Furthermore, chickens vaccinated with ILTV vectors can be easily differentiated from influenza virus-infected animals by the absence of serum antibodies against the AIV nucleoprotein...
  32. doi request reprint Oral immunization of wild boar and domestic pigs with attenuated live vaccine protects against Pseudorabies virus infection
    Christina Maresch
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, 17493 Greifswald Insel Riems, Germany
    Vet Microbiol 161:20-5. 2012
    ..In case increasing PrV infection rates in wild boar may enhance the risk for spillover into domestic pig populations, oral immunization of wild boar against PrV in endemic areas might be a feasible control strategy...
  33. doi request reprint Level of protection of chickens against highly pathogenic H5 avian influenza virus with Newcastle disease virus based live attenuated vector vaccine depends on homology of H5 sequence between vaccine and challenge virus
    Angela Römer-Oberdörfer
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Südufer 10, D 17493 Greifswald Insel Riems, Germany
    Vaccine 26:2307-13. 2008
    ..Our data thus show that application of a vector-based vaccine in the control of influenza may require adaptation of the vaccine to currently circulating viruses...
  34. ncbi request reprint Identification and functional analysis of membrane proteins gD, gE, gI, and pUS9 of Infectious laryngotracheitis virus
    Sophia Pavlova
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, 17493 Greifswald Insel Riems, Germany
    Avian Dis 57:416-26. 2013
    ..Since homologous gene-deletion mutants of other alphaherpesviruses are in use as live vaccines, the generated ILTV recombinants might be also suitable for this application...
  35. pmc The capsid-associated UL25 protein of the alphaherpesvirus pseudorabies virus is nonessential for cleavage and encapsidation of genomic DNA but is required for nuclear egress of capsids
    Barbara G Klupp
    Friedrich Loeffler Institut, Institute of Molecular Biology, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    J Virol 80:6235-46. 2006
    ..Thus, the PrV UL25 protein may represent the hitherto missing trigger that allows primary envelopment preferably of DNA-filled C capsids...
  36. doi request reprint Amino acids adjacent to the haemagglutinin cleavage site are relevant for virulence of avian influenza viruses of subtype H5
    Sandra Gohrbandt
    Friedrich Loeffler Institut, Institute of Molecular Biology, Greifswald Insel Riems, Germany
    J Gen Virol 92:51-9. 2011
    ..These data suggest that evolution of H5 HPAIVs from low-pathogenic precursors, besides acquisition of a polybasic cleavage site, involves adaptation of neighbouring regions...
  37. ncbi request reprint Pathogenicity and immunogenicity of different recombinant Newcastle disease virus clone 30 variants after in ovo vaccination
    Kristina Ramp
    Institute of Molecular Biology, Südufer 10, D 17493 Greifswald Insel Riems, Germany
    Avian Dis 56:208-17. 2012
    ..However, alteration of specific amino acids in F and HN proteins of rNDV did not lead to further attenuation as indicated by their pathogenicity for chicken after in ovo inoculation...
  38. pmc Partial functional complementation of a pseudorabies virus UL25 deletion mutant by herpes simplex virus type 1 pUL25 indicates overlapping functions of alphaherpesvirus pUL25 proteins
    Jana Kuhn
    Friedrich Loeffler Institut, Institute of Molecular Biology, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 82:5725-34. 2008
    ..Thus, our data demonstrate overlapping, although not identical functions of these two conserved herpesvirus proteins, and point to a conserved functional role in herpes virion formation...
  39. pmc Ultrastructural analysis of virion formation and intraaxonal transport of herpes simplex virus type 1 in primary rat neurons
    Alexandra Negatsch
    Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 84:13031-5. 2010
    ..In general, the number of HSV-1 particles in the axons was significantly less than that observed after PrV infection...
  40. pmc Vesicle formation from the nuclear membrane is induced by coexpression of two conserved herpesvirus proteins
    Barbara G Klupp
    Institute of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    Proc Natl Acad Sci U S A 104:7241-6. 2007
    ..This argues for the contribution of cellular factors in this process either recruited from their natural cytoplasmic location or not yet identified as components of the nuclear compartment...
  41. pmc Identification, subviral localization, and functional characterization of the pseudorabies virus UL17 protein
    Barbara G Klupp
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Molecular Biology, Boddenblick 5a, D 17493 Greifswald Insel Riems, Germany
    J Virol 79:13442-53. 2005
    ..In summary, our data indicate that the PrV UL17 protein is an internal nucleocapsid protein necessary for DNA cleavage and packaging but suggest that the protein is not a prominent part of the tegument...
  42. pmc Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification
    Jürgen Stech
    Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    Nucleic Acids Res 36:e139. 2008
    ..In case the PCR amplicon ends are homologous to the plasmid annealing sites only, this method is suitable for cloning of any insert with conserved termini...
  43. pmc Glycoproteins required for entry are not necessary for egress of pseudorabies virus
    Barbara Klupp
    Institute of Molecular Biology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 82:6299-309. 2008
    ..Thus, our data strongly suggest that different fusion mechanisms are active during virus entry and egress...
  44. doi request reprint In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus
    Sophia P Pavlova
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    J Gen Virol 91:847-57. 2010
    ....
  45. pmc The UL48 tegument protein of pseudorabies virus is critical for intracytoplasmic assembly of infectious virions
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D 17498 Insel Riems, Germany
    J Virol 76:6729-42. 2002
    ..However, the UL48 gene of PrV is not absolutely essential, and concomitant deletion of the adjacent tegument protein gene UL49 also did not abolish virus replication in cell culture...
  46. ncbi request reprint Coexpression of avian influenza virus H5 and N1 by recombinant Newcastle disease virus and the impact on immune response in chickens
    Kristina Ramp
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Südufer 10, D 17493 Greifswald Insel Riems, Germany
    Avian Dis 55:413-21. 2011
    ..Furthermore, expression of AIV N1, in addition to AIV H5 by NDV, did not increase protection against HPAIV H5N1...
  47. ncbi request reprint Efficacy of Newcastle disease virus recombinant expressing avian influenza virus H6 hemagglutinin against Newcastle disease and low pathogenic avian influenza in chickens and turkeys
    Diana Schröer
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Südufer 10, D 17493 Greifswald Insel Riems, Germany
    Avian Dis 55:201-11. 2011
    ..Therefore, the vector virus has to be improved to overcome these limitations...
  48. doi request reprint Live vaccination with an H5-hemagglutinin-expressing infectious laryngotracheitis virus recombinant protects chickens against different highly pathogenic avian influenza viruses of the H5 subtype
    Sophia P Pavlova
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    Vaccine 27:5085-90. 2009
    ..Vaccination with HA-expressing ILTV also allowed differentiation of immunized from AIV-infected animals by serological tests for antibodies against influenza virus nucleoprotein...
  49. pmc The UL7 gene of pseudorabies virus encodes a nonessential structural protein which is involved in virion formation and egress
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    J Virol 79:11291-9. 2005
    ..However, neuroinvasion and transneuronal spread of PrV were not abolished in the absence of UL7. Thus, UL7 encodes a virion protein of PrV, which plays a role during virion maturation and egress both in vitro and in vivo...
  50. pmc Highly pathogenic avian influenza virus (H5N1) in frozen duck carcasses, Germany, 2007
    Timm C Harder
    Friedrich Loeffler Institut, Isle of Riems, Germany
    Emerg Infect Dis 15:272-9. 2009
    ..Measures that will prevent such undetected introduction of HPAIV (H5N1) into the food chain are urgently required...
  51. ncbi request reprint Pseudorabies virus particles lacking tegument proteins pUL11 or pUL16 incorporate less full-length pUL36 than wild-type virus, but specifically accumulate a pUL36 N-terminal fragment
    Kathrin Michael
    Institute of Molecular Biology, Friedrich Loeffler Institut, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    J Gen Virol 87:3503-7. 2006
    ..Virions lacking pUL11 or pUL16 incorporated less full-length pUL36 than wild-type particles, but contained increased amounts of an N-terminal fragment of pUL36 that is present only in traces in wild-type virus and the other mutants...
  52. pmc Avian influenza virus hemagglutinins H2, H4, H8, and H14 support a highly pathogenic phenotype
    Jutta Veits
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, 17493 Greifswald Insel Riems, Germany
    Proc Natl Acad Sci U S A 109:2579-84. 2012
    ..Therefore, the restriction of natural HPAIV to serotypes H5 and H7 is likely a result of their unique predisposition for acquisition of a polybasic HA cleavage site...
  53. pmc Entry of pseudorabies virus: an immunogold-labeling study
    Harald Granzow
    Friedrich Loeffler Institut, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    J Virol 79:3200-5. 2005
    ..Thus, the three capsid-associated tegument proteins are prime candidates for viral proteins that interact with cellular motor proteins for transport of nucleocapsids to the nucleus...
  54. pmc Influence of pseudorabies virus proteins on neuroinvasion and neurovirulence in mice
    Robert Klopfleisch
    Institute of Molecular Biology, Friedrich Loeffler Institut, Boddenblick 5a, D 17493 Greifswald Insel Riems, Germany
    J Virol 80:5571-6. 2006
    ..Furthermore, by immunohistochemical detection of infected neurons, the kinetics of viral spread in the murine central nervous system was investigated...
  55. pmc Physical interaction between envelope glycoproteins E and M of pseudorabies virus and the major tegument protein UL49
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498 Insel Riems, Germany
    J Virol 76:8208-17. 2002
    ..In summary, we describe specific interactions between herpesvirus envelope and tegument proteins which may play a role in secondary envelopment during herpesvirus virion maturation...
  56. pmc Efficient incorporation of tegument proteins pUL46, pUL49, and pUS3 into pseudorabies virus particles depends on the presence of pUL21
    Kathrin Michael
    Institute of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    J Virol 81:1048-51. 2007
    ..By the reconstitution of wild-type pUL21 expression to PrV-Ba and the transfer of mutated PrV-Ba pUL21 into wild-type PrV, we demonstrate that this phenotype is due to the mutated pUL21...
  57. pmc Acquisition of a polybasic hemagglutinin cleavage site by a low-pathogenic avian influenza virus is not sufficient for immediate transformation into a highly pathogenic strain
    Olga Stech
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    J Virol 83:5864-8. 2009
    ....
  58. ncbi request reprint Identification and characterization of the pseudorabies virus UL43 protein
    Barbara G Klupp
    Friedrich Loeffler Institut, Institute of Molecular Biology, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    Virology 334:224-33. 2005
    ..Double mutant viruses lacking, in addition to UL43, the multiply membrane spanning glycoproteins K or M did not show a phenotype beyond that observed in the gK and gM single deletion mutants...
  59. doi request reprint Generation and characterization of koi herpesvirus recombinants lacking viral enzymes of nucleotide metabolism
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, 17493 Greifswald Insel Riems, Germany
    Arch Virol 156:1059-63. 2011
    ..Experimental infection of carp indicated attenuation of TK- or dUTPase-deleted KHV, and PCR analysis of tissue samples permitted differentiation of mutant from wild-type virus...
  60. ncbi request reprint Molecular biology of avian infectious laryngotracheitis virus
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    Vet Res 38:261-79. 2007
    ..Since they include immunogenic envelope glycoproteins, these results can contribute to the improvement of virus diagnostics, and to the development of marker vaccines...
  61. doi request reprint H9 avian influenza reassortant with engineered polybasic cleavage site displays a highly pathogenic phenotype in chicken
    Sandra Gohrbandt
    Friedrich Loeffler Institute, Institute of Molecular Biology, Greifswald Insel Riems, Germany
    J Gen Virol 92:1843-53. 2011
    ..23. These results suggest that an HPAIV with a subtype other than H5 or H7 would only emerge under conditions where the HA gene could acquire a polybasic cleavage site and the other viral genes carry additional virulence determinants...
  62. ncbi request reprint Enhancement of pathogenicity of Newcastle disease virus by alteration of specific amino acid residues in the surface glycoproteins F and HN
    Angela Römer-Oberdörfer
    Institute of Molecular Biology, Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    Avian Dis 50:259-63. 2006
    ..5. The HN mutation visibly altered conformation of the protein, resulting in the formation of disulfide-linked HN dimers that may indicate that this HN conformation is beneficial for the virulent phenotype...
  63. ncbi request reprint Molecular analysis of highly pathogenic avian influenza virus of subtype H5N1 isolated from wild birds and mammals in northern Germany
    Siegfried Weber
    Institute of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    J Gen Virol 88:554-8. 2007
    ..In addition, an H5N1 isolate originating from a single outbreak in poultry in Germany was found to be related closely to the H5N1 viruses circulating at that time in the wild-bird population...
  64. pmc Schmallenberg virus as possible ancestor of Shamonda virus
    Katja V Goller
    Friedrich Loeffl er Institut, Greifswald Insel Riems, Germany
    Emerg Infect Dis 18:1644-6. 2012
    ..Results of full-genome and serologic investigations indicate that SBV belongs to the species Sathuperi virus and is a possible ancestor of the reassortant Shamonda virus...
  65. pmc Structural determinants for nuclear envelope localization and function of pseudorabies virus pUL34
    Franziska Schuster
    Institutes of Molecular Biology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    J Virol 86:2079-88. 2012
    ..The replacement of the N-terminal 100 aa (pUL34-LapNT100) had no effect on nuclear envelope localization but abrogated pUL31 binding and function...
  66. pmc Identification of a 709-amino-acid internal nonessential region within the essential conserved tegument protein (p)UL36 of pseudorabies virus
    Sindy Böttcher
    Institutes of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    J Virol 80:9910-5. 2006
    ..Thus, our data indicate that the extreme C terminus of (p)UL36 fulfills an essential role in PrV replication, while a large internal portion of the C-terminal half of the protein is dispensable for replication in cell culture...
  67. pmc Avian influenza virus monitoring in wintering waterbirds in Iran, 2003-2007
    Sasan R Fereidouni
    Friedrich Loeffler Institut, Insel Riems, Germany
    Virol J 7:43. 2010
    ..Samples originated from 6 different provinces representative for the 15 most important wintering sites of migratory waterbirds in Iran...
  68. pmc Intergenotypic replacement of lyssavirus matrix proteins demonstrates the role of lyssavirus M proteins in intracellular virus accumulation
    Stefan Finke
    Friedrich Loeffler Institut, Südufer 10, D 17493 Greifswald Insel Riems, Germany
    J Virol 84:1816-27. 2010
    ..Although the biological function of intracellular assembly and accumulation of viruslike structures in the endoplasmic reticulum remain unclear, the observed differences could contribute to diverse host tropism or pathogenicity...
  69. pmc Composition of pseudorabies virus particles lacking tegument protein US3, UL47, or UL49 or envelope glycoprotein E
    Kathrin Michael
    Institute of Molecular Biology, Friedrich Loeffler Institut, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    J Virol 80:1332-9. 2006
    ..By two-dimensional gel electrophoresis multiple isoforms of host cell-derived heat shock protein 70 and annexins A1 and A2 were also identified as structural components of PrV virions...
  70. ncbi request reprint Recognition of cis-acting elements of infectious haematopoietic necrosis virus and viral hemorrhagic septicemia virus by homologous and heterologous helper proteins
    Bernd Hoffmann
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D 17498 Insel Riems, Germany
    Virus Res 93:79-89. 2003
    ....
  71. doi request reprint Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs
    Elke Lange
    Institute of Infectology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    J Gen Virol 90:2119-23. 2009
    ..i., coinciding with clinical signs. Contact chickens remained uninfected, as judged by the absence of virus excretion, clinical signs and seroconversion...
  72. pmc Highly pathogenic avian influenza virus (H5N1) in experimentally infected adult mute swans
    Donata Kalthoff
    Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    Emerg Infect Dis 14:1267-70. 2008
    ..Adult mute swans are highly susceptible, excrete virus, and can be clinically protected by preexposure immunity...
  73. doi request reprint Systemic influenza virus H5N1 infection in cats after gastrointestinal exposure
    Thomas W Vahlenkamp
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    Influenza Other Respir Viruses 4:379-86. 2010
    ..Highly pathogenic avian influenza virus (HPAIV) H5N1 infections in felids have been reported in several countries. Feeding on infected birds has been suggested as potential source of infection...
  74. doi request reprint Deletion or green fluorescent protein tagging of the pUL35 capsid component of pseudorabies virus impairs virus replication in cell culture and neuroinvasion in mice
    Mirjam Krautwald
    Institute of Molecular Biology, Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
    J Gen Virol 89:1346-51. 2008
    ..Thus, fusion of pUL35 with GFP resulted in a non-functional protein, which has to be considered for the use of corresponding mutants in tracing studies...
  75. pmc Limited susceptibility of chickens, turkeys, and mice to pandemic (H1N1) 2009 virus
    Donata Kalthoff
    Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    Emerg Infect Dis 16:703-5. 2010
    ..We demonstrated that chickens were refractory to infection. However, oculo-oronasally inoculated turkeys and intranasally inoculated mice seroconverted without clinical signs of infection...
  76. doi request reprint Gene expression profiling of Pseudorabies virus (PrV) infected bovine cells by combination of transcript analysis and quantitative proteomic techniques
    Martin Skiba
    Institute of Molecular Biology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    Vet Microbiol 143:14-20. 2010
    ..Application of isotope labelling to cell cultures infected with wild-type PrV-Ka and a US3 protein kinase negative mutant allowed to monitor pUS3-dependent changes in the expression levels of viral proteins pUL29, pUL39 and pUL42...
  77. ncbi request reprint Determination of the complete genomic sequence and analysis of the gene products of the virus of Spring Viremia of Carp, a fish rhabdovirus
    Bernd Hoffmann
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D 17498 Insel Riems, Germany
    Virus Res 84:89-100. 2002
    ..Furthermore, monospecific antisera against the distinct viral proteins were generated. Comparison of genome and protein confirm the assignment of SVCV to the genus Vesiculovirus...
  78. doi request reprint Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing
    Axel Karger
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol Methods 164:116-21. 2010
    ..Spectrum-based phylogenetic analysis showed that clustering was mainly driven by taxonomy and allows the species determination of unknown samples...
  79. ncbi request reprint Budding events in herpesvirus morphogenesis
    Thomas C Mettenleiter
    Friedrich Loeffler Institut, Institute of Molecular Biology, Boddenblick 5a, D 17493 Greifswald Insel Riems, Germany
    Virus Res 106:167-80. 2004
    ..This review summarizes recent advances in our understanding of the two herpesvirus budding events...
  80. doi request reprint Protective efficacy of several vaccines against highly pathogenic H5N1 avian influenza virus under experimental conditions
    Jutta Veits
    Institute of Molecular Biology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Südufer 10, Greifswald Insel Riems, Germany
    Vaccine 26:1688-96. 2008
    ....
  81. pmc Ducks as sentinels for avian influenza in wild birds
    Anja Globig
    Friedrich Loeffler Institute, Greifswald Insel Riems, Germany
    Emerg Infect Dis 15:1633-6. 2009
    ..Infections of sentinel birds with different AIV subtypes confirmed the value of such surveillance for AIV monitoring...
  82. ncbi request reprint [Conventional and alternative housing systems for poultry--point of view of infectious disease medicine]
    Franz Josef Conraths
    Friedrich Loeffler Institut, Institut fur Epidemiologie, Wusterhausen
    Berl Munch Tierarztl Wochenschr 118:186-204. 2005
    ..Since all compounds that had been used for prophylactic or therapeutic purposes in the past have been banned, there is a need to develop new drugs which are safe for animals and humans...
  83. ncbi request reprint The nonessential UL49.5 gene of infectious laryngotracheitis virus encodes an O-glycosylated protein which forms a complex with the non-glycosylated UL10 gene product
    Walter Fuchs
    Friedrich Loeffler Institut, Institute of Molecular Biology, 17493 Greifswald Insel Riems, Germany
    Virus Res 112:108-14. 2005
    ..The resulting virus mutants were isolated and propagated in non-complementing chicken cells, which demonstrated that the UL10 and UL49.5 genes are not essential for in vitro replication of ILTV...
  84. ncbi request reprint The "Friedrich-Loeffler-Institut": past, present and future of research in infectious diseases of animals
    Franz J Conraths
    Friedrich Loeffler Institut, Institut fur Epidemiologie, Bundesforschungsinstitut für Tiergesundheit, Wusterhausen, Germany
    Berl Munch Tierarztl Wochenschr 118:354-64. 2005
    ....
  85. doi request reprint Efficacy of three inactivated vaccines against bluetongue virus serotype 8 in sheep
    Michael Eschbaumer
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    Vaccine 27:4169-75. 2009
    ..Only one vaccinated animal was very weakly positive in the real-time RT-PCR at day 10 after challenge infection, and one seronegative sheep in one of the vaccine groups was not protected...
  86. ncbi request reprint [Recombinant viruses of poultry as vector vaccines against fowl plague]
    Walter Fuchs
    Institut fur Molekularbiologie, Friedrich Loeffler Institut, Bundesforschungsinstitut für Tiergesundheit, Greifswald Insel Riems
    Berl Munch Tierarztl Wochenschr 119:160-6. 2006
    ..An H5-expressing FPV recombinant is already in use in Central America and Southeast Asia but without accompanying marker diagnostics. Advantages and disadvantages of the different viral vectors are discussed...
  87. doi request reprint Simultaneous one-tube full-length amplification of the NA, NP, M, and NS genes of influenza A viruses for reverse genetics
    Anne Kreibich
    Friedrich Loeffler Institut, Bundesforschungsinstitut für Tiergesundheit, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol Methods 159:308-10. 2009
    ..By avoiding separate assays for several viral genes, this parallel PCR steps up rapid universal cloning of influenza A virus genes further...
  88. pmc Herpesvirus assembly and egress
    Thomas C Mettenleiter
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498 Insel Riems, Germany
    J Virol 76:1537-47. 2002
  89. pmc Complete, annotated sequence of the pseudorabies virus genome
    Barbara G Klupp
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17493 Greifswald Insel Riems, Germany
    J Virol 78:424-40. 2004
    ..Many of these features are conserved in the alphaherpesvirus subfamily and have important implications for gene array analyses...
  90. pmc Pseudorabies virus UL36 tegument protein physically interacts with the UL37 protein
    Barbara G Klupp
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498 Insel Riems, Germany
    J Virol 76:3065-71. 2002
    ..Zhou, D. Chen, J. Jakana, F. J. Rixon, and W. Chiu, J. Virol. 73:3210-3218, 1999), with the UL37 protein is an important early step in tegumentation during virion morphogenesis in the cytoplasm...
  91. pmc In vitro and in vivo relevance of infectious laryngotracheitis virus gJ proteins that are expressed from spliced and nonspliced mRNAs
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institut, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    J Virol 79:705-16. 2005
    ..Thus, gJ deletion mutants of ILTV might be usable as attenuated live-virus vaccines. Furthermore, the gJ gene might constitute a reliable marker for serological discrimination between vaccinated and field virus-infected chickens...
  92. ncbi request reprint Characterization of a recombinant Newcastle disease virus expressing the green fluorescent protein
    Ines Engel-Herbert
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498, Insel Riems, Germany
    J Virol Methods 108:19-28. 2003
    ..Taken together, these data demonstrate the use of GFP-expressing recombinant NDV for analysis of NDV dissemination and pathogenesis and indicate the potential usefulness of NDV as a vaccine vector...
  93. pmc Essential function of the pseudorabies virus UL36 gene product is independent of its interaction with the UL37 protein
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institut, Boddenblick 5a, D 17493 Greifswald Insel Riems, Germany
    J Virol 78:11879-89. 2004
    ..Furthermore, UL36 possesses an essential function during virus replication which is independent of its ability to bind the UL37 protein...
  94. pmc The pseudorabies virus US3 protein is a component of primary and of mature virions
    Harald Granzow
    Institutes of Infectology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17493 Greifswald Insel Riems, Germany
    J Virol 78:1314-23. 2004
    ....
  95. pmc The interacting UL31 and UL34 gene products of pseudorabies virus are involved in egress from the host-cell nucleus and represent components of primary enveloped but not mature virions
    Walter Fuchs
    Institutes of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498 Insel Riems, Germany
    J Virol 76:364-78. 2002
    ....
  96. pmc Identification and characterization of the pseudorabies virus tegument proteins UL46 and UL47: role for UL47 in virion morphogenesis in the cytoplasm
    Martina Kopp
    Institutes of Molecular Biology Infectology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498 Insel Riems, Germany
    J Virol 76:8820-33. 2002
    ..In summary, we identified the PrV UL46 and UL47 proteins and show that the UL47 protein plays an important role in virion assembly in the cytoplasm...
  97. pmc Experimental infection and natural contact exposure of dogs with avian influenza virus (H5N1)
    Matthias Giese
    Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    Emerg Infect Dis 14:308-10. 2008
    ..Infected dogs showed increased body temperatures, viral RNA in pharyngeal swabs, and seroconversion but not fatal disease...
  98. pmc Egress of alphaherpesviruses
    Thomas C Mettenleiter
    J Virol 80:1610-1; author reply 1611-2. 2006
  99. pmc Characterization of White bream virus reveals a novel genetic cluster of nidoviruses
    Heike Schütze
    The Queen s University of Belfast, School of Biomedical Sciences, 97 Lisburn Road, Belfast BT9 7BL, United Kingdom
    J Virol 80:11598-609. 2006
    ..The study adds to the amazing diversity of nidoviruses and appeals for a more extensive characterization of nonmammalian nidoviruses to better understand the evolution of these largest known RNA viruses...
  100. pmc Quantitative whole-cell proteome analysis of pseudorabies virus-infected cells
    Martin Skiba
    Institute of Molecular Biology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 82:9689-99. 2008
    ..As a common pattern for all these proteins, a mass shift in favor of the more acidic isoforms was observed, suggesting the involvement of viral or cellular kinases...
  101. pmc Complex formation between the UL16 and UL21 tegument proteins of pseudorabies virus
    Barbara G Klupp
    Institute of Molecular Biology, Friedrich Loeffler Institut, Boddenblick 5a, D 17493 Greifswald Insel Riems, Germany
    J Virol 79:1510-22. 2005
    ..In summary, our results uncover a novel interaction between conserved herpesvirus tegument proteins that increases the complexity of the intricate network of protein-protein interactions involved in herpesvirus morphogenesis...