S O Mueller

Summary

Affiliation: Merck KGaA
Country: Germany

Publications

  1. ncbi request reprint Effects of cell culture conditions on primary rat hepatocytes-cell morphology and differential gene expression
    Gregor Tuschl
    Institute of Toxicology, Molecular Toxicology, Merck KGaA, 64271 Darmstadt, Germany
    Toxicology 218:205-15. 2006
  2. ncbi request reprint Overview of in vitro tools to assess the estrogenic and antiestrogenic activity of phytoestrogens
    Stefan O Mueller
    Merck KGaA, Molecular Toxicology, Frankfurter Strasse 250, 64293, Darmstadt, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 777:155-65. 2002
  3. ncbi request reprint Molecular determinants of the stereoselectivity of agonist activity of estrogen receptors (ER) alpha and beta
    Stefan O Mueller
    Laboratories of Reproductive and Developmental Toxicology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:12255-62. 2003
  4. ncbi request reprint Activation of estrogen receptor alpha and ERbeta by 4-methylbenzylidene-camphor in human and rat cells: comparison with phyto- and xenoestrogens
    Stefan O Mueller
    Institute of Toxicology, Merck KGaA, 64271 Darmstadt, Germany
    Toxicol Lett 142:89-101. 2003
  5. ncbi request reprint Endogenous estrogen receptor beta is transcriptionally active in primary ovarian cells from estrogen receptor knockout mice
    Stefan O Mueller
    Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Steroids 69:681-6. 2004
  6. ncbi request reprint Phytoestrogens and their human metabolites show distinct agonistic and antagonistic properties on estrogen receptor alpha (ERalpha) and ERbeta in human cells
    Stefan O Mueller
    Molecular Toxicology, Institute of Toxicology, Merck KGaA, Darmstadt, Germany
    Toxicol Sci 80:14-25. 2004
  7. ncbi request reprint Estrogen receptors and endocrine diseases: lessons from estrogen receptor knockout mice
    S O Mueller
    Merck KGaA, Institute of Toxicology, Darmstadt, Germany
    Curr Opin Pharmacol 1:613-9. 2001
  8. ncbi request reprint Alternatives in pharmaceutical toxicology: global and focussed approaches--two case studies
    Stefan O Mueller
    Molecular Toxicology, Institute of Toxicology, Merck KGaA, Darmstadt, Germany
    ALTEX 24:117-24. 2007
  9. doi request reprint Species-specific activation of nuclear receptors correlates with the response of liver drug metabolizing enzymes to EMD 392949 in vitro
    Stefan O Mueller
    Merck KGaA, Merck Serono, Non Clinical Development, Toxicology, Darmstadt, Germany
    Toxicol Lett 193:120-3. 2010
  10. ncbi request reprint Xenoestrogens: mechanisms of action and detection methods
    Stefan O Mueller
    Molecular Toxicology, Institute of Toxicology, Merck KGaA, PO Box, 64271 Darmstadt, Germany
    Anal Bioanal Chem 378:582-7. 2004

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Effects of cell culture conditions on primary rat hepatocytes-cell morphology and differential gene expression
    Gregor Tuschl
    Institute of Toxicology, Molecular Toxicology, Merck KGaA, 64271 Darmstadt, Germany
    Toxicology 218:205-15. 2006
    ..In conclusion, culturing primary rat hepatocytes in a sandwich between two layers of gelled collagen and in a serum-free medium formulation, appears to be most suitable for long-term in vitro hepatotoxicity screening...
  2. ncbi request reprint Overview of in vitro tools to assess the estrogenic and antiestrogenic activity of phytoestrogens
    Stefan O Mueller
    Merck KGaA, Molecular Toxicology, Frankfurter Strasse 250, 64293, Darmstadt, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 777:155-65. 2002
    ....
  3. ncbi request reprint Molecular determinants of the stereoselectivity of agonist activity of estrogen receptors (ER) alpha and beta
    Stefan O Mueller
    Laboratories of Reproductive and Developmental Toxicology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:12255-62. 2003
    ..These data demonstrate that a single residue in the ligand-binding domain determines the stereoselectivity of ERalpha and ERbeta for indenestrol ligands and that IA-R shows cell type selectivity through ERbeta...
  4. ncbi request reprint Activation of estrogen receptor alpha and ERbeta by 4-methylbenzylidene-camphor in human and rat cells: comparison with phyto- and xenoestrogens
    Stefan O Mueller
    Institute of Toxicology, Merck KGaA, 64271 Darmstadt, Germany
    Toxicol Lett 142:89-101. 2003
    ....
  5. ncbi request reprint Endogenous estrogen receptor beta is transcriptionally active in primary ovarian cells from estrogen receptor knockout mice
    Stefan O Mueller
    Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Steroids 69:681-6. 2004
    ..In conclusion, this study demonstrates that endogenously expressed ERbeta is capable of regulating gene transcription independent of ERalpha...
  6. ncbi request reprint Phytoestrogens and their human metabolites show distinct agonistic and antagonistic properties on estrogen receptor alpha (ERalpha) and ERbeta in human cells
    Stefan O Mueller
    Molecular Toxicology, Institute of Toxicology, Merck KGaA, Darmstadt, Germany
    Toxicol Sci 80:14-25. 2004
    ..Due to their abundance and (anti)-estrogenic potencies, the soy-derived isoflavones, coumestrol, resveratrol, and zearalenone would appear to have the potential for effectively functioning as endocrine disruptors...
  7. ncbi request reprint Estrogen receptors and endocrine diseases: lessons from estrogen receptor knockout mice
    S O Mueller
    Merck KGaA, Institute of Toxicology, Darmstadt, Germany
    Curr Opin Pharmacol 1:613-9. 2001
    ..Recent studies with estrogen receptor knockout mice have helped to unravel the role of the estrogen receptors in brain degeneration, osteoporosis, cardiovascular diseases and obesity...
  8. ncbi request reprint Alternatives in pharmaceutical toxicology: global and focussed approaches--two case studies
    Stefan O Mueller
    Molecular Toxicology, Institute of Toxicology, Merck KGaA, Darmstadt, Germany
    ALTEX 24:117-24. 2007
    ..These examples demonstrate that an intelligent testing strategy, using alternative methods, can enable a meaningful safety assessment for humans by adding a ''tailor-made'' range of technologies to ''classic'' toxicological methods...
  9. doi request reprint Species-specific activation of nuclear receptors correlates with the response of liver drug metabolizing enzymes to EMD 392949 in vitro
    Stefan O Mueller
    Merck KGaA, Merck Serono, Non Clinical Development, Toxicology, Darmstadt, Germany
    Toxicol Lett 193:120-3. 2010
    ..These findings support the tight correlation of species-specific nuclear receptor activation with P450 induction and foster the use of nuclear receptor activation as a complementary screen to identify cytochrome P450 inducers...
  10. ncbi request reprint Xenoestrogens: mechanisms of action and detection methods
    Stefan O Mueller
    Molecular Toxicology, Institute of Toxicology, Merck KGaA, PO Box, 64271 Darmstadt, Germany
    Anal Bioanal Chem 378:582-7. 2004
    ..How screening assays and mechanistic studies can aid in human risk assessment for potential endocrine-active compounds is discussed also...
  11. ncbi request reprint Immortalized testis cell lines from estrogen receptor (ER) alpha knock-out and wild-type mice expressing functional ERalpha or ERbeta
    S O Mueller
    Laboratory of Reproductive and Developmental Toxicology Receptor Biology Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA
    J Androl 22:652-64. 2001
    ..These cell lines with and without functional ERalpha or ERbeta enable the analyses of ER subtype-specific responses and their function in testicular cell signaling, morphogenesis, and neoplasia...
  12. doi request reprint Gene expression profiling in Ishikawa cells: a fingerprint for estrogen active compounds
    Kathleen Boehme
    Merck KGaA, Merck Serono, NCD Toxicology, Early and Explanatory Toxicology, 64271 Darmstadt, Germany
    Toxicol Appl Pharmacol 236:85-96. 2009
    ..The characteristic expression fingerprints and the identified subset of putative marker genes can be used for screening chemicals with an unknown mode of action and for predicting their potential to exert endocrine disrupting effects...
  13. ncbi request reprint Estrogen receptor alpha and beta subtype expression and transactivation capacity are differentially affected by receptor-, hsp90- and immunophilin-ligands in human breast cancer cells
    Angélique Gougelet
    UMR CNRS 8612, 5, rue J B Clement, 92296 Chatenay Malabry, France
    J Steroid Biochem Mol Biol 94:71-81. 2005
    ....
  14. ncbi request reprint Oestrogen receptors pathways to oestrogen responsive elements: the transactivation function-1 acts as the keystone of oestrogen receptor (ER)beta-mediated transcriptional repression of ERalpha
    Angélique Gougelet
    UMR CNRS 8612, 5 rue J B Clement, 92296 Chatenay Malabry, France
    J Steroid Biochem Mol Biol 104:110-22. 2007
    ..Finally, the ratio ERalpha/ERbeta constitutes one decisive parameters to orientate the transcriptional mechanism of a target gene in the presence of agonist as well as of antagonist ligands...
  15. ncbi request reprint Characterization of the species-specificity of peroxisome proliferators in rat and human hepatocytes
    Manuel Ammerschlaeger
    Molecular Toxicology, Institute of Toxicology, Merck KGaA, 64271 Darmstadt, Germany
    Toxicol Sci 78:229-40. 2004
    ..Nevertheless, our results showed that human hepatocytes limit the extent of peroxisome proliferation regardless of PPARalpha expression...
  16. ncbi request reprint Human reporter gene assays: transcriptional activity of the androgen receptor is modulated by the cellular environment and promoter context
    Stephanie Simon
    Institute of Toxicology, Molecular Toxicology, Merck KGaA, 64271 Darmstadt, Germany
    Toxicology 220:90-103. 2006
    ..Furthermore, this study highlights the need to investigate the (anti-) androgenic activity of compounds in dependence of the cellular and promoter context...
  17. ncbi request reprint Mammary gland development in adult mice requires epithelial and stromal estrogen receptor alpha
    Stefan O Mueller
    Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Endocrinology 143:2357-65. 2002
    ..Immunostaining for ER alpha and PR in the mammary outgrowths supported the view that both stromal and epithelial ER alpha, in cooperation with epithelial PR, govern mammary gland development in adult mice...
  18. doi request reprint Species-specific toxicity of diclofenac and troglitazone in primary human and rat hepatocytes
    Birthe Lauer
    Merck KGaA, Merck Serono, Non Clinical Development, Early and Explanatory Toxicology, Darmstadt, Germany
    Chem Biol Interact 179:17-24. 2009
    ....
  19. ncbi request reprint Primary hepatocytes as a model to analyze species-specific toxicity and drug metabolism
    Gregor Tuschl
    Merck KGaA, Merck Serono, Non Clinical Development, Early and Explanatory Toxicology, Frankfurter Str 250, 64293 Darmstadt, Germany
    Expert Opin Drug Metab Toxicol 4:855-70. 2008
    ..Among the most frequent adverse effects are drug-induced liver injury generated by species-specific susceptibilities (e.g., in xenobiotic metabolism and/or the occurrence of idiosyncratic drug hepatotoxicity)...
  20. doi request reprint Serum-free collagen sandwich cultures of adult rat hepatocytes maintain liver-like properties long term: a valuable model for in vitro toxicity and drug-drug interaction studies
    Gregor Tuschl
    Merck KGaA, Merck Serono, Non Clinical Development, Early and Explanatory Toxicology, Frankfurter Strasse 250, Darmstadt, Germany
    Chem Biol Interact 181:124-37. 2009
    ..Taken together, the serum-free collagen sandwich culture of primary rat hepatocytes maintained liver-like features over 10 days and is therefore a suitable model for long-term toxicity and drug-drug interaction studies...
  21. doi request reprint Replacement of in vivo acute oral toxicity studies by in vitro cytotoxicity methods: opportunities, limits and regulatory status
    Ute Ukelis
    Global Regulatory Affairs, Merck Serono, Merck KGaA, 64271 Darmstadt, Germany
    Regul Toxicol Pharmacol 51:108-18. 2008
    ..We have then analyzed opportunities and drawbacks for future implementation of in vitro test methods, with particular focus on industrial use...
  22. doi request reprint Species differences in the response of liver drug-metabolizing enzymes to (S)-4-O-tolylsulfanyl-2-(4-trifluormethyl-phenoxy)-butyric acid (EMD 392949) in vivo and in vitro
    Lysiane Richert
    Laboratoire de Biologie Cellulaire, l Institut Fédératif de Recherches 133, Facultéde Médecine et de Pharmacie, Besancon, France
    Drug Metab Dispos 36:702-14. 2008
    ..This study also clearly highlighted major differences between primates and rodents in the regulation of major inducible P450s, with evidence of CYP3A and CYP2C inducibility by PPARalpha agonists in monkeys and humans...
  23. doi request reprint Activation of P53 in HepG2 cells as surrogate to detect mutagens and promutagens in vitro
    Kathleen Boehme
    Merck KGaA, Merck Serono, Toxicology, 64293 Darmstadt, Germany
    Toxicol Lett 198:272-81. 2010
    ..In summary, our results indicate that P53 activation in HepG2 cells combined with a MAS can be used for the identification of new (pro)genotoxicants...
  24. ncbi request reprint Increased cell proliferation is associated with genomic instability: elevated micronuclei frequencies in estradiol-treated human ovarian cancer cells
    Helga Stopper
    Department of Toxicology, University of Wurzburg, Versbacher Strasse 9, D 97078 Wurzburg, Germany
    Mutagenesis 18:243-7. 2003
    ..We hypothesize that hormone-specific forcing of responsive cells through the cell cycle leads to an override of checkpoints operating under homeostatic control of the cell cycle, resulting in genomic instability...