Alexander Mellmann

Summary

Country: Germany

Publications

  1. pmc Characterization of clonal relatedness among the natural population of Staphylococcus aureus strains by using spa sequence typing and the BURP (based upon repeat patterns) algorithm
    Alexander Mellmann
    Institute for Hygiene, University Hospital Munster, Robert Koch Str 41, 48149 Munster, Germany
    J Clin Microbiol 46:2805-8. 2008
  2. pmc Sequencing and staphylococci identification
    Alexander Mellmann
    University Hospital Munster, Munster, Germany
    Emerg Infect Dis 12:333-6. 2006
  3. pmc Phylogenetic analysis of enterohemorrhagic Escherichia coli O157, Germany, 1987-2008
    Christian Jenke
    Institute for Hygiene, Munster, Germany
    Emerg Infect Dis 16:610-6. 2010
  4. pmc Identification of intermediate in evolutionary model of enterohemorrhagic Escherichia coli O157
    Christian Jenke
    Institute for Hygiene and National Consulting Laboratory on Hemolytic Uremic Syndrome, Munster, Germany
    Emerg Infect Dis 18:582-8. 2012
  5. pmc Heteropathogenic virulence and phylogeny reveal phased pathogenic metamorphosis in Escherichia coli O2:H6
    Martina Bielaszewska
    Institute of Hygiene, University of Munster, Munster, Germany
    EMBO Mol Med 6:347-57. 2014
  6. pmc Based Upon Repeat Pattern (BURP): an algorithm to characterize the long-term evolution of Staphylococcus aureus populations based on spa polymorphisms
    Alexander Mellmann
    Institute for Hygiene, University Hospital Munster, Munster, Germany
    BMC Microbiol 7:98. 2007
  7. pmc Recycling of Shiga toxin 2 genes in sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM
    Alexander Mellmann
    Institute of Hygiene, University of Munster, Robert Koch Str 41, 48149 Munster, Germany
    Appl Environ Microbiol 74:67-72. 2008
  8. pmc Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria
    A Mellmann
    Institute for Hygiene, University Hospital Muenster, Muenster D 48149, Germany
    J Clin Microbiol 46:1946-54. 2008
  9. ncbi request reprint Enterohemorrhagic Escherichia coli in human infection: in vivo evolution of a bacterial pathogen
    Alexander Mellmann
    Institute for Hygiene, National Consulting Laboratory on Hemolytic Uremic Syndrome and IZKF Munster, Munster, Germany
    Clin Infect Dis 41:785-92. 2005
  10. pmc Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli
    Alexander Mellmann
    Institute for Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, Munster, Germany
    Emerg Infect Dis 14:1287-90. 2008

Detail Information

Publications64

  1. pmc Characterization of clonal relatedness among the natural population of Staphylococcus aureus strains by using spa sequence typing and the BURP (based upon repeat patterns) algorithm
    Alexander Mellmann
    Institute for Hygiene, University Hospital Munster, Robert Koch Str 41, 48149 Munster, Germany
    J Clin Microbiol 46:2805-8. 2008
    ..BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%)...
  2. pmc Sequencing and staphylococci identification
    Alexander Mellmann
    University Hospital Munster, Munster, Germany
    Emerg Infect Dis 12:333-6. 2006
    ..This database correctly identified 55 clinical staphylococcal isolates; all were correctly identified at the species level. At the subspecies level, rpoB misidentified only 2 isolates...
  3. pmc Phylogenetic analysis of enterohemorrhagic Escherichia coli O157, Germany, 1987-2008
    Christian Jenke
    Institute for Hygiene, Munster, Germany
    Emerg Infect Dis 16:610-6. 2010
    ..Specific MLVA profiles with an evolutionary persistence were identified, particularly within sorbitol-fermenting EHEC O157:H-.These pathogens belonged to the same MLVA cluster. Our findings indicate successful persistence of this clone...
  4. pmc Identification of intermediate in evolutionary model of enterohemorrhagic Escherichia coli O157
    Christian Jenke
    Institute for Hygiene and National Consulting Laboratory on Hemolytic Uremic Syndrome, Munster, Germany
    Emerg Infect Dis 18:582-8. 2012
    ..The animal origin of this intermediate emphasizes the value of nonhuman reservoirs in the clarification of the evolution of human pathogens...
  5. pmc Heteropathogenic virulence and phylogeny reveal phased pathogenic metamorphosis in Escherichia coli O2:H6
    Martina Bielaszewska
    Institute of Hygiene, University of Munster, Munster, Germany
    EMBO Mol Med 6:347-57. 2014
    ..coli undergoes phased metamorphosis into another. By understanding the evolutionary mechanisms of bacterial pathogens, rational strategies for counteracting their detrimental effects on humans can be developed. ..
  6. pmc Based Upon Repeat Pattern (BURP): an algorithm to characterize the long-term evolution of Staphylococcus aureus populations based on spa polymorphisms
    Alexander Mellmann
    Institute for Hygiene, University Hospital Munster, Munster, Germany
    BMC Microbiol 7:98. 2007
    ..BURP was subsequently evaluated with a strain collection reflecting the breadth of diversity of S. aureus (JCM 2002; 40:4544)...
  7. pmc Recycling of Shiga toxin 2 genes in sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM
    Alexander Mellmann
    Institute of Hygiene, University of Munster, Robert Koch Str 41, 48149 Munster, Germany
    Appl Environ Microbiol 74:67-72. 2008
    ..The ability to recycle stx(2), a critical virulence trait, makes SF E. coli O157:NM strains ephemeral EHEC that can exist as stx-negative variants during certain phases of their life cycle...
  8. pmc Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria
    A Mellmann
    Institute for Hygiene, University Hospital Muenster, Muenster D 48149, Germany
    J Clin Microbiol 46:1946-54. 2008
    ..Thus, MALDI-TOF MS-based species identification of nonfermenting bacteria provided accurate and reproducible results within 10 min without any substantial costs for consumables...
  9. ncbi request reprint Enterohemorrhagic Escherichia coli in human infection: in vivo evolution of a bacterial pathogen
    Alexander Mellmann
    Institute for Hygiene, National Consulting Laboratory on Hemolytic Uremic Syndrome and IZKF Munster, Munster, Germany
    Clin Infect Dis 41:785-92. 2005
    ..To investigate genetic changes in EHEC during the course of human infection, we analyzed consecutive stool samples and shed isolates from patients with HUS, focusing on the genes encoding Shiga toxin (stx) and intimin (eae)...
  10. pmc Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli
    Alexander Mellmann
    Institute for Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, Munster, Germany
    Emerg Infect Dis 14:1287-90. 2008
    ..1% of these strains clustered in 5 STs. From all STs and serotypes identified, we established a reference panel of EHEC associated with HUS (HUSEC collection)...
  11. pmc Phylogeny and disease association of Shiga toxin-producing Escherichia coli O91
    Alexander Mellmann
    Institute of Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, Munster, Germany
    Emerg Infect Dis 15:1474-7. 2009
    ..We identified 10 specific sequence types (ST) and 4 distinct clonal groups. ST442 was significantly associated with hemolytic uremic syndrome...
  12. ncbi request reprint [Evidence-based infection control methods using spa genotyping for MRSA spread in hospitals]
    A Mellmann
    Institut fur Hygiene, Universitatsklinikum Munster
    Dtsch Med Wochenschr 130:1364-8. 2005
    ..In this study we evaluated protein A ( spa) gene repeat sequence analysis for MRSA typing in a hospital...
  13. pmc High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria
    A Mellmann
    Institut fur Hygiene, Universitatsklinikum Munster, Robert Koch Str 41, D 48149 Munster, Germany
    J Clin Microbiol 47:3732-4. 2009
    ..75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample)...
  14. ncbi request reprint Shiga toxin-negative attaching and effacing Escherichia coli: distinct clinical associations with bacterial phylogeny and virulence traits and inferred in-host pathogen evolution
    Martina Bielaszewska
    Institute for Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University of Munster, Wernigerode, Germany
    Clin Infect Dis 47:208-17. 2008
    ..coli and cause diarrhea worldwide. However, it is unknown whether there are bacterial lineage-specific human disease phenotypes. We compared stx-/EAF- AEEC recovered from patients (mostly children) with bloody and nonbloody diarrhea...
  15. ncbi request reprint Structural and functional differences between disease-associated genes of enterohaemorrhagic Escherichia coli O111
    Wenlan Zhang
    Institute for Hygiene, and the National Consulting Laboratory on Haemolytic Uraemic Syndrome, University Hospital Munster, Robert Koch Strasse 41, D 48149 Munster, Germany
    Int J Med Microbiol 297:17-26. 2007
    ..The divergence within EHEC O111 makes it possible to subtype these emerging pathogens in the laboratory thereby providing a basis for further investigations into their ecological niches and survival capabilities...
  16. pmc Shiga toxin-mediated hemolytic uremic syndrome: time to change the diagnostic paradigm?
    Martina Bielaszewska
    Institute of Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University of Munster, Munster, Germany
    PLoS ONE 2:e1024. 2007
    ..However, the frequency of stx-negative/eae-positive E. coli in stools of HUS patients and the clinical significance of such strains are unknown...
  17. doi request reprint Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study
    Martina Bielaszewska
    Institute for Hygiene and the National Consulting Laboratory for Haemolytic Uraemic Syndrome, University of Munster, Munster, Germany
    Lancet Infect Dis 11:671-6. 2011
    ..We analysed virulence profiles and relevant phenotypes of outbreak isolates recovered in our laboratory...
  18. ncbi request reprint Phylogeny and phenotypes of clinical and environmental Shiga toxin-producing Escherichia coli O174
    Wenlan Zhang
    Institute of Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University of Munster, 48149, Munster, Germany
    Environ Microbiol 16:963-76. 2014
    ..Our study provides a basis for O174 subtyping, unravels considerable genotypic and phenotypic heterogeneity and sheds light to potential environmental and animal reservoirs...
  19. ncbi request reprint Prevalence, virulence profiles, and clinical significance of Shiga toxin-negative variants of enterohemorrhagic Escherichia coli O157 infection in humans
    Alexander W Friedrich
    National Consulting Laboratory on Hemolytic Uremic Syndrome, Institute for Hygiene, University of Munster, Munster, Germany
    Clin Infect Dis 45:39-45. 2007
    ..coli O157-infected patients. It is unknown if loss of the stx gene during the early stage of an enterohemorrhagic E. coli infection prevents HUS, or if absence of the stx gene from E. coli O157 reduces or ablates virulence...
  20. pmc Shiga toxin gene loss and transfer in vitro and in vivo during enterohemorrhagic Escherichia coli O26 infection in humans
    Martina Bielaszewska
    Institute for Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University of Munster, Germany
    Appl Environ Microbiol 73:3144-50. 2007
    ..The suggested occurrence of this process in the human intestine has diagnostic, clinical, epidemiological, and evolutionary implications...
  21. pmc Shiga toxin, cytolethal distending toxin, and hemolysin repertoires in clinical Escherichia coli O91 isolates
    Martina Bielaszewska
    Institut fur Hygiene, Universitat Munster, Munster, Germany
    J Clin Microbiol 47:2061-6. 2009
    ....
  22. pmc Chromosomal dynamism in progeny of outbreak-related sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM
    Martina Bielaszewska
    Institut fur Hygiene, Universitat Munster, Robert Koch Str 41, 48149 Munster, Germany
    Appl Environ Microbiol 72:1900-9. 2006
    ..The variability of PFGE patterns for the progeny of a single colony must be considered when interpreting PFGE patterns in SF EHEC O157-associated outbreaks...
  23. pmc Automated DNA sequence-based early warning system for the detection of methicillin-resistant Staphylococcus aureus outbreaks
    Alexander Mellmann
    Institute for Hygiene, University Hospital Munster, Munster, Germany
    PLoS Med 3:e33. 2006
    ..Prompt identification of an MRSA epidemic is crucial for the control of an outbreak. In this study we evaluated various early warning algorithms for the detection of an MRSA cluster...
  24. pmc Distribution and phylogeny of immunoglobulin-binding protein G in Shiga toxin-producing Escherichia coli and its association with adherence phenotypes
    Viktor Merkel
    Institute for Hygiene and Interdisciplinary Center of Clinical Research IZKF, University of Munster, Robert Koch Str 41, 48149 Munster, Germany
    Infect Immun 78:3625-36. 2010
    ..We propose two possible evolutionary scenarios for eibG in STEC: a clonal development of eibG in strains with the same phylogenetic background or horizontal transfer of eibG between phylogenetically unrelated STEC strains...
  25. pmc Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species
    Karsten Becker
    Institute of Medical Microbiology, University of Munster, D 48149 Munster, Germany
    J Clin Microbiol 42:4988-95. 2004
    ..In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants...
  26. pmc Chromosomal instability in enterohaemorrhagic Escherichia coli O157:H7: impact on adherence, tellurite resistance and colony phenotype
    Martina Bielaszewska
    Institute of Hygiene and the National Consulting Laboratory on Haemolytic Uraemic Syndrome, University of Munster, Robert Koch Str 41, 48149 Munster, Germany
    Mol Microbiol 79:1024-44. 2011
    ..Spontaneous deletions affecting Tel(R) OIs contribute to EHEC O157 genome plasticity and might impair virulence and/or fitness...
  27. pmc Phylogeny, clinical associations, and diagnostic utility of the pilin subunit gene (sfpA) of sorbitol-fermenting, enterohemorrhagic Escherichia coli O157:H-
    Alexander W Friedrich
    Institut fur Hygiene, Universitatsklinikum Munster, Munster, Germany
    J Clin Microbiol 42:4697-701. 2004
    ..None of the other stool samples was positive by the sfpA PCR; 59 of these stool samples contained EHEC O157:H7. The sfpA gene can be recommended as a target for screening for SF E. coli O157:H-...
  28. pmc Detection and characterization of the fimbrial sfp cluster in enterohemorrhagic Escherichia coli O165:H25/NM isolates from humans and cattle
    Martina Bielaszewska
    Institut fur Hygiene, Universitat Munster, Robert Koch Str 41, 48149 Munster, Germany
    Appl Environ Microbiol 75:64-71. 2009
    ..The reasons for the convergence of pathogenic EHEC strains on a suite of virulence loci remain unknown...
  29. doi request reprint Enterohemorrhagic Escherichia coli O26:H11/H-: a new virulent clone emerges in Europe
    Martina Bielaszewska
    Institute for Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University of Munster, Wernigerode, Germany
    Clin Infect Dis 56:1373-81. 2013
    ..We determined phylogeny, clonal structure, and plasmid gene profiles of the isolates and portray geographic and temporal distribution of the different subgroups...
  30. pmc Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) as causes of human infection and colonization in Germany
    Robin Köck
    Institute of Hygiene, University Hospital Munster, Munster, Germany
    PLoS ONE 8:e55040. 2013
    ..Our findings indicate that LA-MRSA are a major cause for human infection and stress the need for close surveillance. Although LA-MRSA CC398 predominates, the occurrence of putative LA-MRSA from other clonal lineages should be monitored...
  31. pmc Evaluation of multiple-locus variable number of tandem repeats analysis for typing livestock-associated methicillin-resistant Staphylococcus aureus
    Karin M Brandt
    Institute of Hygiene, University Hospital Munster, Munster, Germany
    PLoS ONE 8:e54425. 2013
    ..We therefore compared MLVA and S. aureus protein A (spa) typing for characterizing porcine MRSA from distinct Dutch and German farms...
  32. pmc Population dynamics among methicillin-resistant Staphylococcus aureus isolates in Germany during a 6-year period
    Frieder Schaumburg
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    J Clin Microbiol 50:3186-92. 2012
    ..The ongoing epidemiological shift and changes of MRSA sources create a need for revision of guidelines for MRSA infection control and treatment...
  33. pmc Subtypes of the plasmid-encoded serine protease EspP in Shiga toxin-producing Escherichia coli: distribution, secretion, and proteolytic activity
    Jens Brockmeyer
    Institut fur Hygiene, Universitat Munster, Robert Koch Str 41, 48149 Munster, Germany
    Appl Environ Microbiol 73:6351-9. 2007
    ..The presence of proteolytically active EspP in EHEC serogroups O157, O26, O111, and O145, which are bona fide human pathogens, suggests that EspP might play a role as an EHEC virulence factor...
  34. pmc Characterization of Escherichia coli strains isolated from patients with diarrhea in Sao Paulo, Brazil: identification of intermediate virulence factor profiles by multiplex PCR
    Ariane Liebchen
    Institut für Infektiologie, Zentrum fur Molekularbiologie der Entzundung, Von Esmarch Str 56, D 48149 Munster, Germany
    J Clin Microbiol 49:2274-8. 2011
    ..Interestingly, most strains were enteroaggregative E. coli (EAEC) strains, followed by atypical EPEC (ATEC) strains. Classical enteropathogenic E. coli (EPEC) strains were not detected...
  35. pmc Shiga toxin 2e-producing Escherichia coli isolates from humans and pigs differ in their virulence profiles and interactions with intestinal epithelial cells
    Anne Katharina Sonntag
    Institute of Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University Hospital Munster, Robert Koch Str 41, Munster, Germany
    Appl Environ Microbiol 71:8855-63. 2005
    ..Our data demonstrate that E. coli isolates producing Shiga toxin 2e have imported specific virulence and fitness determinants which allow them to adapt to the specific hosts in which they cause various forms of disease...
  36. ncbi request reprint Enterohaemorrhagic Escherichia coli O26:H11/H-: a human pathogen in emergence
    Martina Bielaszewska
    Institut fur Hygiene, und Nationales Konsiliarlaboratorium für Hämolytisch Urämisches Syndrom, Universitat Munster, Robert Koch Str 41, 48149 Munster, Germany
    Berl Munch Tierarztl Wochenschr 120:279-87. 2007
    ....
  37. pmc Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection
    Francisco Toval
    Institute for Hygiene, University of Munster, Munster, Germany
    J Clin Microbiol 52:407-18. 2014
    ..coli (ExPEC) and IPEC virulence determinants in some isolates demonstrates the marked genome plasticity of E. coli and calls for a reevaluation of the strict pathotype classification of EAEC. ..
  38. pmc Effects of antibiotics on Shiga toxin 2 production and bacteriophage induction by epidemic Escherichia coli O104:H4 strain
    Martina Bielaszewska
    Institute of Hygiene and the National Consulting Laboratory for Hemolytic Uremic Syndrome, University of Munster, Munster, Germany
    Antimicrob Agents Chemother 56:3277-82. 2012
    ..We await further analysis of the epidemic to determine if usage of these agents was associated with an altered risk of developing HUS...
  39. pmc Evolution of enterohemorrhagic escherichia coli O26 based on single-nucleotide polymorphisms
    Stefan Bletz
    Institute of Hygiene, University of Munster, Germany
    Genome Biol Evol 5:1807-16. 2013
    ..In addition, the SNP-CC analysis may help to assess a risk in infected individuals for the progression to HUS and to implement more specific infection control measures...
  40. pmc Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools
    Wenlan Zhang
    Institute for Hygiene and the National Consulting Laboratory for Hemolytic Uremic Syndrome, University of Munster, Munster, Germany
    J Clin Microbiol 50:1752-4. 2012
    ..It is a rapid, sensitive, and specific tool for detecting EHEC O104:H4 in human stools...
  41. ncbi request reprint Distribution of capsular and surface polysaccharide serotypes of Staphylococcus aureus
    Christof von Eiff
    Institute of Medical Microbiology, University Hospital of Munster, 48149 Munster, Germany
    Diagn Microbiol Infect Dis 58:297-302. 2007
    ....
  42. ncbi request reprint Evidence for cost reduction based on pre-admission MRSA screening in general surgery
    Ricarda Diller
    Department of General Surgery, University Hospital of Munster, Waldeyerstrasse 1, 48149 Munster, Germany
    Int J Hyg Environ Health 211:205-12. 2008
    ..In conclusion, pre-admission screening for MRSA is an effective method to reduce the hospital burden of MRSA-colonized patients and the savings due to consistent decolonization before elective admission outweigh the costs of screening...
  43. pmc Sequence-based typing of flaB is a more stable screening tool than typing of flaA for monitoring of Campylobacter populations
    Alexander Mellmann
    Institut fur Hygiene, Universitatsklinikum Munster, Munster, Germany
    J Clin Microbiol 42:4840-2. 2004
    ..886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination...
  44. pmc The epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in Germany
    Robin Köck
    Institut fur Hygiene, Universitatsklinikum Munster, Germany
    Dtsch Arztebl Int 108:761-7. 2011
    ..Moreover, animal reservoirs are increasingly considered to represent an important source of human MRSA acquisition. In this review article the authors describe the current epidemiological situation of MRSA in Germany...
  45. ncbi request reprint Epidemiology and pathogenesis of enterohaemorrhagic Escherichia coil
    Helge Karch
    Institut fur Hygiene, und Nationales Konsiliarlaboratorium für Hämolytisch Urämisches Syndrom, Universitat Munster, Munster, Germany
    Berl Munch Tierarztl Wochenschr 122:417-24. 2009
    ....
  46. pmc Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology
    Alexander Mellmann
    Institute of Hygiene, University Munster, Munster, Germany
    PLoS ONE 6:e22751. 2011
    ..In conclusion, rapid next-generation technologies facilitated prospective whole genome characterization in the early stages of an outbreak...
  47. doi request reprint High burden of extended-spectrum β-lactamase-producing Enterobacteriaceae in Gabon
    Frieder Schaumburg
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    J Antimicrob Chemother 68:2140-3. 2013
    ..Our objective was to assess the burden of rectal ESBL-E colonization and associated risk factors in Gabon, Central Africa..
  48. ncbi request reprint Intrahost genome alterations in enterohemorrhagic Escherichia coli
    Alexander Mellmann
    Institute of Hygiene, Interdisciplinary Center for Clinical Research, and the National Consulting Laboratory on Hemolytic Uremic Syndrome, University of Munster, Munster, Germany
    Gastroenterology 136:1925-38. 2009
    ..The intensive study of human enteric factors that induce or modulate pathogen chromosome instability could provide important information about host-microbial interactions...
  49. doi request reprint Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection
    Francisco Toval
    Institute for Hygiene, University of Munster, Munster, Germany
    J Clin Microbiol 52:407-18. 2014
    ..coli (ExPEC) and IPEC virulence determinants in some isolates demonstrates the marked genome plasticity of E. coli and calls for a reevaluation of the strict pathotype classification of EAEC. ..
  50. pmc Comparison of multilocus variable-number tandem-repeat analysis and multilocus sequence typing for differentiation of hemolytic-uremic syndrome-associated Escherichia coli (HUSEC) collection strains
    Christian Jenke
    Institute of Hygiene and National Consulting Laboratory for Hemolytic Uremic Syndrome, University Hospital Munster, Robert Koch Strasse 41, 48149 Munster, Germany
    J Clin Microbiol 49:3644-6. 2011
    ..Although MLVA--like MLST--was highly discriminatory (index of diversity, 0.988 versus 0.984), a low level of concordance demonstrated the limited ability of MLVA to reflect long-term evolutionary events...
  51. ncbi request reprint Cross-border comparison of the admission prevalence and clonal structure of meticillin-resistant Staphylococcus aureus
    R Kock
    Institute of Hygiene, University Hospital Munster, Munster, Germany
    J Hosp Infect 71:320-6. 2009
    ..In patients lacking all risk factors assessed, spa types t011 and t034 were predominant (P<0.001)...
  52. pmc High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study
    M Aires-de-Sousa
    Department of Periodontology, University Hospital Munster, Waldeyerstrasse 30, D 48149 Munster, Germany
    J Clin Microbiol 44:619-21. 2006
    ..aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature...
  53. pmc Enterohemorrhagic Escherichia coli hemolysin employs outer membrane vesicles to target mitochondria and cause endothelial and epithelial apoptosis
    Martina Bielaszewska
    Institute of Hygiene, University of Munster, Munster, Germany
    PLoS Pathog 9:e1003797. 2013
    ..The OMV-mediated intracellular delivery represents a newly recognized mechanism for a bacterial toxin to enter host cells in order to target mitochondria. ..
  54. ncbi request reprint Evaluation of RIDOM, MicroSeq, and Genbank services in the molecular identification of Nocardia species
    Alexander Mellmann
    Institut fur Hygiene, Universitatsklinikum Munster, 48149 Munster, Germany
    Int J Med Microbiol 293:359-70. 2003
    ..The RIDOM service (http://www.ridom-rdna.de/) is in the process of making available a comprehensive and high-quality database for bacterial identification purposes and provides excellent results for the majority of Nocardia isolates...
  55. doi request reprint Microbiologic contamination and time efficiency of use of automatic MDCT injectors with prefilled syringes: Results of a clinical investigation
    Boris Buerke
    Department of Clinical Radiology, University of Muenster, Germany
    AJR Am J Roentgenol 194:299-303. 2010
    ..The purpose of this study was to evaluate the microbiologic contamination and time efficiency associated with routine clinical use of single-use prefilled disposable syringes for contrast administration...
  56. doi request reprint Microbiologic contamination of automatic injectors at MDCT: experimental and clinical investigations
    Boris Buerke
    Department of Clinical Radiology, University of Muenster, Albert Schweitzer Strasse 33, 48149 Muenster, Germany
    AJR Am J Roentgenol 191:W283-7. 2008
    ..The purpose of this study was to evaluate in an experimental setting and in clinical routine the risk of microbiologic contamination of the syringes of injectors used to administer contrast agent and saline solution for MDCT...
  57. pmc Variation of the polymorphic region X of the protein A gene during persistent airway infection of cystic fibrosis patients reflects two independent mechanisms of genetic change in Staphylococcus aureus
    Barbara C Kahl
    Institute of Medical Microbiology, University of Muenster, Domagkstr 10, 48149 Muenster, Germany
    J Clin Microbiol 43:502-5. 2005
    ..Independent mutational events were observed eight times in 142 isolates: four deletions, two duplications of repeats, and two point mutations...
  58. ncbi request reprint Urease genes in non-O157 Shiga toxin-producing Escherichia coli: mostly silent but valuable markers for pathogenicity
    A W Friedrich
    Institut fur Hygiene, Universitatsklinikum Munster, Robert Koch Strasse 41, 48149 Munster, Germany
    Clin Microbiol Infect 12:483-6. 2006
    ..The silencing of urease expression could not be linked to a stop codon in ureD. The frequent occurrence of ure genes in eae-positive STEC isolates makes them valuable markers for virulence...
  59. ncbi request reprint Bacterial whole-genome sequencing revisited: portable, scalable, and standardized analysis for typing and detection of virulence and antibiotic resistance genes
    Shana R Leopold
    Institute of Hygiene, University of Munster, Munster, Germany
    J Clin Microbiol 52:2365-70. 2014
    ..WGS coupled with allelic profiling provided a high-resolution method that can be implemented as regular screening for effective infection control. ..
  60. ncbi request reprint [EUREGIO-projekt MRSA-net Twente/Münsterland. Creation of a regional network to combat MRSA]
    I Daniels-Haardt
    Landesinstitut für den Offentlichen Gesundheitsdienst NRW, Munster, Germany
    Gesundheitswesen 68:674-8. 2006
    ....
  61. ncbi request reprint Staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens
    Konrad Trulzsch
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Munich, Germany
    Int J Syst Evol Microbiol 57:1543-8. 2007
    ..Due to differences in the results of physiological and chemotaxonomic investigations and DNA-DNA hybridization data, strain A6664 was not included in the description of the novel species...
  62. pmc Acinetobacter septicus sp. nov. association with a nosocomial outbreak of bacteremia in a neonatal intensive care unit
    Abdullah Kilic
    Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018 Ankara, Turkey
    J Clin Microbiol 46:902-8. 2008
    ..We propose the designation of Acinetobacter septicus sp. nov. for these isolates, with isolate AK001 as the type strain...
  63. ncbi request reprint Staphylococcal chromosomal cassette mec type I, spa type, and expression of Pls are determinants of reduced cellular invasiveness of methicillin-resistant Staphylococcus aureus isolates
    Cornelia Werbick
    Institute of Medical Microbiology, University Hospital of Munster, Germany
    J Infect Dis 195:1678-85. 2007
    ..In contrast, the spa type-deduced multilocus sequence typing clonal complex (MLST-CC) was not able to predict the invasiveness of MRSA strains equally well. The underlying mechanism remains to be elucidated...
  64. ncbi request reprint Ridom TraceEdit: a DNA trace editor and viewer
    Jorg Rothganger
    Ridom GmbH, 97082 Wurzburg, Germany
    Bioinformatics 22:493-4. 2006
    ..TraceEdit displays the chromatogram files from Applied Biosystems automated sequencers and files in the Staden SCF format. Incorrect base calls can be edited and saved...