R Melcher

Summary

Country: Germany

Publications

  1. doi request reprint LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability
    Ralph Melcher
    Department of Medicine II, Division of Gastroenterology, University of Wurzburg, Oberdurrbacher Strasse 6, 97080 Wurzburg, Germany
    Carcinogenesis 32:636-42. 2011
  2. ncbi request reprint SKY and genetic fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1
    Ralph Melcher
    Institute of Human Genetics, University of Wuerzburg, Gastrolabor Bau 4, Joseph Schneider Str 2, 97074 Wuerzburg, Germany
    Cancer Genet Cytogenet 158:84-7. 2005
  3. ncbi request reprint SNP-Array genotyping and spectral karyotyping reveal uniparental disomy as early mutational event in MSS- and MSI-colorectal cancer cell lines
    R Melcher
    Department of Medicine II, Division of Gastroenterology, University of Wurzburg, Wurzburg, Germany
    Cytogenet Genome Res 118:214-21. 2007
  4. ncbi request reprint Cytokine-activated degradation of inhibitory kappaB protein alpha is inhibited by the short-chain fatty acid butyrate
    H Luhrs
    Department of Medicine, University of Wurzburg, Germany
    Int J Colorectal Dis 16:195-201. 2001
  5. pmc Expression of the cathelicidin LL-37 is modulated by short chain fatty acids in colonocytes: relevance of signalling pathways
    J Schauber
    Department of Medicine, Division of Gastroenterology, University of Wurzburg, Germany
    Gut 52:735-41. 2003
  6. doi request reprint Improved metabolic control after 12-week dietary intervention with low glycaemic isomalt in patients with type 2 diabetes mellitus
    I Holub
    University of Wurzburg, Department of Medicine II, Division of Gastroenterology, 97080 Wurzburg, Germany
    Horm Metab Res 41:886-92. 2009
  7. ncbi request reprint Butyrate and aspirin in combination have an enhanced effect on apoptosis in human colorectal cancer cells
    T Menzel
    Division of Gastroenterology, Department of Medicine, University of Wurzburg, Germany
    Eur J Cancer Prev 11:271-81. 2002
  8. ncbi request reprint Butyrate inhibits interleukin-1-mediated nuclear factor-kappa B activation in human epithelial cells
    H Luhrs
    Department of Medicine, University of Wurzburg, Germany
    Dig Dis Sci 46:1968-73. 2001
  9. ncbi request reprint Spectral karyotype analysis of colon cancer cell lines of the tumor suppressor and mutator pathway
    R Melcher
    Department of Medicine, University of Wurzburg, Germany
    Cytogenet Genome Res 98:22-8. 2002
  10. ncbi request reprint Effects of isomalt consumption on gastrointestinal and metabolic parameters in healthy volunteers
    A Gostner
    Department of Medicine, University of Wurzburg, Josef Schneider Strasse 2, D 97080 Wurzburg, Germany
    Br J Nutr 94:575-81. 2005

Collaborators

Detail Information

Publications21

  1. doi request reprint LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability
    Ralph Melcher
    Department of Medicine II, Division of Gastroenterology, University of Wurzburg, Oberdurrbacher Strasse 6, 97080 Wurzburg, Germany
    Carcinogenesis 32:636-42. 2011
    ..The aim of our study was to analyse tissue from MSI and MSS CRC for the existence of (frequently) deleted chromosomal regions and tumor suppressor genes located therein...
  2. ncbi request reprint SKY and genetic fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1
    Ralph Melcher
    Institute of Human Genetics, University of Wuerzburg, Gastrolabor Bau 4, Joseph Schneider Str 2, 97074 Wuerzburg, Germany
    Cancer Genet Cytogenet 158:84-7. 2005
    ..Our findings highlight the importance of molecular and cytogenetic characterization of established cell lines to avoid drawing misleading conclusions from the original findings...
  3. ncbi request reprint SNP-Array genotyping and spectral karyotyping reveal uniparental disomy as early mutational event in MSS- and MSI-colorectal cancer cell lines
    R Melcher
    Department of Medicine II, Division of Gastroenterology, University of Wurzburg, Wurzburg, Germany
    Cytogenet Genome Res 118:214-21. 2007
    ..Further studies are warranted to identify relevant genes in the respective regions and to further verify the results presented here...
  4. ncbi request reprint Cytokine-activated degradation of inhibitory kappaB protein alpha is inhibited by the short-chain fatty acid butyrate
    H Luhrs
    Department of Medicine, University of Wurzburg, Germany
    Int J Colorectal Dis 16:195-201. 2001
    ..The anti-inflammatory effects of butyrate may at least in part be mediated by an inhibition of IkappaBalpha mediated activation of NF-kappaB...
  5. pmc Expression of the cathelicidin LL-37 is modulated by short chain fatty acids in colonocytes: relevance of signalling pathways
    J Schauber
    Department of Medicine, Division of Gastroenterology, University of Wurzburg, Germany
    Gut 52:735-41. 2003
    ..In particular, SCFA modulate mucosal immune functions. The antimicrobial cathelicidin LL-37 is expressed by colon epithelial cells. In the present study the effect of SCFA on LL-37 expression was investigated...
  6. doi request reprint Improved metabolic control after 12-week dietary intervention with low glycaemic isomalt in patients with type 2 diabetes mellitus
    I Holub
    University of Wurzburg, Department of Medicine II, Division of Gastroenterology, 97080 Wurzburg, Germany
    Horm Metab Res 41:886-92. 2009
    ..The present study is in agreement with findings of previous reported studies in human subjects demonstrating beneficial effects of low glycaemic diets on glucose metabolism in patients with diabetes mellitus type 2...
  7. ncbi request reprint Butyrate and aspirin in combination have an enhanced effect on apoptosis in human colorectal cancer cells
    T Menzel
    Division of Gastroenterology, Department of Medicine, University of Wurzburg, Germany
    Eur J Cancer Prev 11:271-81. 2002
    ..The combination resulted in a stronger decrease in the expression of PCNA and cdk2. Our data suggest that the anticarcinogenic effect of aspirin might effectively be augmented by combination with the short-chain fatty acid butyrate...
  8. ncbi request reprint Butyrate inhibits interleukin-1-mediated nuclear factor-kappa B activation in human epithelial cells
    H Luhrs
    Department of Medicine, University of Wurzburg, Germany
    Dig Dis Sci 46:1968-73. 2001
    ..These data indicate that inhibition of IL-1beta-induced NF-kappaB activation by butyrate does not require an intact IkappaB alpha protein...
  9. ncbi request reprint Spectral karyotype analysis of colon cancer cell lines of the tumor suppressor and mutator pathway
    R Melcher
    Department of Medicine, University of Wurzburg, Germany
    Cytogenet Genome Res 98:22-8. 2002
    ..To examine the chromosomal differences in microsatellite-stable (MSS) and -unstable (MSI) tumors in detail, we analyzed MSS (Caco-2, Colo-205, SW948) and MSI (HCT-15, HCT-116, LoVo) cell lines by spectral karyotyping (SKY)...
  10. ncbi request reprint Effects of isomalt consumption on gastrointestinal and metabolic parameters in healthy volunteers
    A Gostner
    Department of Medicine, University of Wurzburg, Josef Schneider Strasse 2, D 97080 Wurzburg, Germany
    Br J Nutr 94:575-81. 2005
    ..This suggests that isomalt is well tolerated and that consumption of isomalt does not impair metabolic function or induce hypercalciuria. In addition, the study data indicate that isomalt could be useful in improving bowel function...
  11. ncbi request reprint [Special gastrointestinal problems of elderly patients]
    H Luhrs
    Oberarzt der Medizinischen Klinik und Poliklinik II, Universitatsklinikum Wurzburg
    MMW Fortschr Med 149:29-32. 2007
    ..The possibility of tumours should always be considered in the differential diagnosis of elderly patients. The diagnosis and treatment of elderly patients for gastrointestinal diseases are no different from that of other age groups...
  12. ncbi request reprint [The role of the diet in the prevention of gastrointestinal tumors]
    T Kudlich
    Medizinische Klinik und Poliklinik II, Universitatsklinikum Wurzburg
    MMW Fortschr Med 149:36-8. 2007
    ..An additional protective effect is associated with a balanced diet, physical activity, preservation of normal weight, avoidance of smoking, and moderation in the amount of alcohol consumed...
  13. ncbi request reprint [Effect of nutrition factors on the pathogenesis of colorectal carcinoma]
    W Scheppach
    Schwerpunkt Gastroenterologie, Medizinische Universitätsklinik Würzburg
    Zentralbl Chir 125:5-7. 2000
    ..g., dietary fiber) are degraded in the colon to short-chain fatty acids which exhibit protective effects in experimental models of carcinogenesis. The putative protection from vitamins and trace elements merits further attention...
  14. ncbi request reprint Butyrate inhibits leukocyte adhesion to endothelial cells via modulation of VCAM-1
    Thomas Menzel
    Division of Gastroenterology, Department of Medicine, University of Wurzburg, Wurzburg, Germany
    Inflamm Bowel Dis 10:122-8. 2004
    ..We investigated whether the anti-inflammatory properties of butyrate are mediated via the modulation of VCAM and ICAM on human endothelial cells...
  15. ncbi request reprint Butyrate-enhanced TNFalpha-induced apoptosis is associated with inhibition of NF-kappaB
    Hardi Lührs
    Department of Medicine, University of Wurzburg, Germany
    Anticancer Res 22:1561-8. 2002
    ..In this context, the inhibition of NF-kappaB activation and subsequent modulation of (NF-kappaB)-dependent genes was assessed..
  16. ncbi request reprint Plasmid vectors with a 5'-hybrid intron facilitate high-level glycoprotein expression in CHO-cells
    Ralph Melcher
    Department of Medicine, University of Wuerzburg, Gastrolabor Bau 4, Joseph Schneider Str 2, 97080 Wuerzburg, Germany
    Biochim Biophys Acta 1575:49-53. 2002
    ..Using conventional tissue culturing of CHO cells stably transfected with this vector, we were able to isolate glycosylated lysozyme with a yield of 4.5 mg per liter of spent medium...
  17. ncbi request reprint Modulation of HMG-N2 binding to chromatin by butyrate-induced acetylation in human colon adenocarcinoma cells
    Hardi Lührs
    Department of Medicine, University of Wurzburg, Wurzburg, Germany
    Int J Cancer 97:567-73. 2002
    ..Modulation of nonhistone chromatin proteins, like the ubiquitous HMG-N2 proteins, may be partly responsible for the wide range of butyrate-associated effects...
  18. pmc Novel findings on the metabolic effects of the low glycaemic carbohydrate isomaltulose (Palatinose)
    Ines Holub
    Department of Medicine II, Division of Gastroenterology, University of Wurzburg, Wurzburg, Germany
    Br J Nutr 103:1730-7. 2010
    ..Regular iso consumption is well tolerated also in subjects with increased risk for vascular diseases...
  19. ncbi request reprint Establishment of a colonic adenoma cell line (GEKI-2): spectral karyotype analysis and functional characterization
    Thomas Menzel
    Division of Gastroenterology, Department of Medicine, University of Wurzburg, Josef Schneider Strasse 2, 97080, Wurzburg, Germany
    Int J Colorectal Dis 19:12-7. 2004
    ..Thus, an in vitro model consisting of cells stemming from an colon adenoma would be desirable. However, establishing such a cell line has proven difficult...
  20. ncbi request reprint Butyrate inhibits NF-kappaB activation in lamina propria macrophages of patients with ulcerative colitis
    H Luhrs
    Dept of Medicine, Institute of Pathology, University of Wurzburg, Germany
    Scand J Gastroenterol 37:458-66. 2002
    ..Clinical trials suggest anti-inflammatory effects of locally administered butyrate in UC. The potential effects of butyrate on NF-kappaB activation in lamina propria macrophages of UC patients were investigated...
  21. ncbi request reprint Human rectal mucosal gene expression after consumption of digestible and non-digestible carbohydrates
    Jürgen Schauber
    Department of Medicine II, Division of Gastroenterology, University of Wurzburg, Wurzburg, Germany
    Mol Nutr Food Res 50:1006-12. 2006
    ..Dietary intervention with the low digestible isomalt compared with the digestible sucrose did not affect gene expression in the lining rectal mucosa...